UniProtKB - Q15059 (BRD3_HUMAN)
Bromodomain-containing protein 3
BRD3
Functioni
Chromatin reader that recognizes and binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling and interaction with transcription factors (PubMed:18406326, PubMed:27105114).
Regulates transcription by promoting the binding of the transcription factor GATA1 to its targets (By similarity).
By similarity2 PublicationsGO - Molecular functioni
- chromatin binding Source: UniProtKB
- lysine-acetylated histone binding Source: UniProtKB
- nucleosomal histone binding Source: GO_Central
GO - Biological processi
- chromatin remodeling Source: GO_Central
- regulation of transcription, DNA-templated Source: GO_Central
- regulation of transcription by RNA polymerase II Source: UniProtKB
Keywordsi
Molecular function | Chromatin regulator |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | Q15059 |
SignaLinki | Q15059 |
SIGNORi | Q15059 |
Names & Taxonomyi
Protein namesi | Recommended name: Bromodomain-containing protein 3Alternative name(s): RING3-like protein |
Gene namesi | Name:BRD3 Synonyms:KIAA0043, RING3L |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:1104, BRD3 |
MIMi | 601541, gene |
neXtProti | NX_Q15059 |
VEuPathDBi | HostDB:ENSG00000169925 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Note: Detected on chromatin.By similarity
Keywords - Cellular componenti
NucleusPathology & Biotechi
Involvement in diseasei
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 647 – 648 | Breakpoint for translocation to form BDR3-NUTM1 fusion protein1 Publication | 2 |
Organism-specific databases
DisGeNETi | 8019 |
OpenTargetsi | ENSG00000169925 |
PharmGKBi | PA25415 |
Miscellaneous databases
Pharosi | Q15059, Tchem |
Chemistry databases
ChEMBLi | CHEMBL1795186 |
GuidetoPHARMACOLOGYi | 2725 |
Genetic variation databases
BioMutai | BRD3 |
DMDMi | 12643726 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000211181 | 2 – 726 | Bromodomain-containing protein 3Add BLAST | 725 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources | 1 | |
Modified residuei | 263 | PhosphoserineCombined sources | 1 | |
Modified residuei | 281 | PhosphoserineCombined sources | 1 | |
Cross-linki | 414 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 563 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q15059 |
jPOSTi | Q15059 |
MassIVEi | Q15059 |
MaxQBi | Q15059 |
PaxDbi | Q15059 |
PeptideAtlasi | Q15059 |
PRIDEi | Q15059 |
ProteomicsDBi | 60414 [Q15059-1] 60415 [Q15059-2] |
PTM databases
GlyGeni | Q15059, 5 sites, 1 O-linked glycan (5 sites) |
iPTMneti | Q15059 |
PhosphoSitePlusi | Q15059 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000169925, Expressed in forebrain and 259 other tissues |
ExpressionAtlasi | Q15059, baseline and differential |
Genevisiblei | Q15059, HS |
Organism-specific databases
HPAi | ENSG00000169925, Low tissue specificity |
Interactioni
Subunit structurei
Interacts (via bromo domain 1) with GATA1 acetylated at 'Lys-312' and 'Lys-315'.
Interacts (via bromo domain 1) with GATA2 acetylated on lysine residues (By similarity).
Interacts (via bromo domains) with acetylated lysine residues on the N-terminus of histone H2A, H2B, H3 and H4 (in vitro).
By similarity2 PublicationsBinary interactionsi
Q15059
With | #Exp. | IntAct |
---|---|---|
MYC [P01106] | 3 | EBI-1383460,EBI-447544 |
Isoform 2 [Q15059-2]
With | #Exp. | IntAct |
---|---|---|
KPNA3 [O00505] | 3 | EBI-13468085,EBI-358297 |
GO - Molecular functioni
- lysine-acetylated histone binding Source: UniProtKB
- nucleosomal histone binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 113715, 230 interactors |
DIPi | DIP-39755N |
IntActi | Q15059, 23 interactors |
MINTi | Q15059 |
STRINGi | 9606.ENSP00000305918 |
Chemistry databases
BindingDBi | Q15059 |
Miscellaneous databases
RNActi | Q15059, protein |
Structurei
Secondary structure
3D structure databases
SASBDBi | Q15059 |
SMRi | Q15059 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q15059 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 51 – 123 | Bromo 1PROSITE-ProRule annotationAdd BLAST | 73 | |
Domaini | 326 – 398 | Bromo 2PROSITE-ProRule annotationAdd BLAST | 73 | |
Domaini | 562 – 644 | NETPROSITE-ProRule annotationAdd BLAST | 83 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 35 | DisorderedSequence analysisAdd BLAST | 35 | |
Regioni | 78 – 80 | Acetylated histone H3 binding1 Publication | 3 | |
Regioni | 149 – 169 | DisorderedSequence analysisAdd BLAST | 21 | |
Regioni | 237 – 305 | DisorderedSequence analysisAdd BLAST | 69 | |
Regioni | 421 – 462 | DisorderedSequence analysisAdd BLAST | 42 | |
Regioni | 477 – 575 | DisorderedSequence analysisAdd BLAST | 99 | |
Regioni | 637 – 726 | DisorderedSequence analysisAdd BLAST | 90 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 453 – 524 | Sequence analysisAdd BLAST | 72 | |
Coiled coili | 645 – 684 | Sequence analysisAdd BLAST | 40 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 14 – 29 | Pro residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 247 – 266 | Polar residuesSequence analysisAdd BLAST | 20 | |
Compositional biasi | 273 – 305 | Basic and acidic residuesSequence analysisAdd BLAST | 33 | |
Compositional biasi | 498 – 524 | Basic and acidic residuesSequence analysisAdd BLAST | 27 | |
Compositional biasi | 535 – 551 | Polar residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 652 – 673 | Basic and acidic residuesSequence analysisAdd BLAST | 22 | |
Compositional biasi | 697 – 726 | Polar residuesSequence analysisAdd BLAST | 30 |
Domaini
Keywords - Domaini
Bromodomain, Coiled coil, RepeatPhylogenomic databases
eggNOGi | KOG1474, Eukaryota |
GeneTreei | ENSGT00940000153385 |
HOGENOMi | CLU_001499_0_4_1 |
InParanoidi | Q15059 |
OMAi | CYAFNPD |
PhylomeDBi | Q15059 |
TreeFami | TF317345 |
Family and domain databases
CDDi | cd05497, Bromo_Brdt_I_like, 1 hit cd05498, Bromo_Brdt_II_like, 1 hit |
Gene3Di | 1.20.1270.220, 1 hit 1.20.920.10, 2 hits |
InterProi | View protein in InterPro IPR043508, Bromo_Brdt_I IPR043509, Bromo_Brdt_II IPR001487, Bromodomain IPR036427, Bromodomain-like_sf IPR018359, Bromodomain_CS IPR027353, NET_dom IPR038336, NET_sf |
Pfami | View protein in Pfam PF17035, BET, 1 hit PF00439, Bromodomain, 2 hits |
PRINTSi | PR00503, BROMODOMAIN |
SMARTi | View protein in SMART SM00297, BROMO, 2 hits |
SUPFAMi | SSF47370, SSF47370, 2 hits |
PROSITEi | View protein in PROSITE PS00633, BROMODOMAIN_1, 2 hits PS50014, BROMODOMAIN_2, 2 hits PS51525, NET, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSTATTVAPA GIPATPGPVN PPPPEVSNPS KPGRKTNQLQ YMQNVVVKTL
60 70 80 90 100
WKHQFAWPFY QPVDAIKLNL PDYHKIIKNP MDMGTIKKRL ENNYYWSASE
110 120 130 140 150
CMQDFNTMFT NCYIYNKPTD DIVLMAQALE KIFLQKVAQM PQEEVELLPP
160 170 180 190 200
APKGKGRKPA AGAQSAGTQQ VAAVSSVSPA TPFQSVPPTV SQTPVIAATP
210 220 230 240 250
VPTITANVTS VPVPPAAAPP PPATPIVPVV PPTPPVVKKK GVKRKADTTT
260 270 280 290 300
PTTSAITASR SESPPPLSDP KQAKVVARRE SGGRPIKPPK KDLEDGEVPQ
310 320 330 340 350
HAGKKGKLSE HLRYCDSILR EMLSKKHAAY AWPFYKPVDA EALELHDYHD
360 370 380 390 400
IIKHPMDLST VKRKMDGREY PDAQGFAADV RLMFSNCYKY NPPDHEVVAM
410 420 430 440 450
ARKLQDVFEM RFAKMPDEPV EAPALPAPAA PMVSKGAESS RSSEESSSDS
460 470 480 490 500
GSSDSEEERA TRLAELQEQL KAVHEQLAAL SQAPVNKPKK KKEKKEKEKK
510 520 530 540 550
KKDKEKEKEK HKVKAEEEKK AKVAPPAKQA QQKKAPAKKA NSTTTAGRQL
560 570 580 590 600
KKGGKQASAS YDSEEEEEGL PMSYDEKRQL SLDINRLPGE KLGRVVHIIQ
610 620 630 640 650
SREPSLRDSN PDEIEIDFET LKPTTLRELE RYVKSCLQKK QRKPFSASGK
660 670 680 690 700
KQAAKSKEEL AQEKKKELEK RLQDVSGQLS SSKKPARKEK PGSAPSGGPS
710 720
RLSSSSSSES GSSSSSGSSS DSSDSE
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA9J571 | A9J571_HUMAN | Bromodomain-containing protein 3 | BRD3 | 74 | Annotation score: | ||
A9J575 | A9J575_HUMAN | Bromodomain-containing protein 3 | BRD3 | 20 | Annotation score: |
Sequence cautioni
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_041913 | 36 | T → N in a renal clear cell carcinoma sample; somatic mutation. 1 Publication | 1 | |
Natural variantiVAR_041914 | 161 | A → T in a gastric adenocarcinoma sample; somatic mutation. 1 Publication | 1 | |
Natural variantiVAR_041915 | 172 | A → V1 PublicationCorresponds to variant dbSNP:rs34609592Ensembl. | 1 | |
Natural variantiVAR_041916 | 435 | K → Q1 PublicationCorresponds to variant dbSNP:rs36093130Ensembl. | 1 | |
Natural variantiVAR_041917 | 441 | R → H1 PublicationCorresponds to variant dbSNP:rs56017928Ensembl. | 1 | |
Natural variantiVAR_041918 | 447 | S → P1 PublicationCorresponds to variant dbSNP:rs55754444Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_010247 | 549 – 556 | QLKKGGKQ → DHFLTCGV in isoform 2. 1 Publication | 8 | |
Alternative sequenceiVSP_010248 | 557 – 726 | Missing in isoform 2. 1 PublicationAdd BLAST | 170 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D26362 mRNA Translation: BAA05393.2 Different initiation. AY513270 mRNA Translation: AAS82952.1 AL445931 Genomic DNA No translation available. CH471090 Genomic DNA Translation: EAW88113.1 CH471090 Genomic DNA Translation: EAW88114.1 BC032124 mRNA Translation: AAH32124.1 Z81330 Genomic DNA No translation available. |
CCDSi | CCDS6980.1 [Q15059-1] |
RefSeqi | NP_031397.1, NM_007371.3 [Q15059-1] XP_006717354.1, XM_006717291.2 [Q15059-1] XP_011517354.1, XM_011519052.2 [Q15059-1] |
Genome annotation databases
Ensembli | ENST00000303407; ENSP00000305918; ENSG00000169925 ENST00000371834; ENSP00000360900; ENSG00000169925 [Q15059-2] |
GeneIDi | 8019 |
KEGGi | hsa:8019 |
MANE-Selecti | ENST00000303407.12; ENSP00000305918.6; NM_007371.4; NP_031397.1 |
UCSCi | uc004cew.4, human [Q15059-1] |
Keywords - Coding sequence diversityi
Alternative splicing, Chromosomal rearrangementSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D26362 mRNA Translation: BAA05393.2 Different initiation. AY513270 mRNA Translation: AAS82952.1 AL445931 Genomic DNA No translation available. CH471090 Genomic DNA Translation: EAW88113.1 CH471090 Genomic DNA Translation: EAW88114.1 BC032124 mRNA Translation: AAH32124.1 Z81330 Genomic DNA No translation available. |
CCDSi | CCDS6980.1 [Q15059-1] |
RefSeqi | NP_031397.1, NM_007371.3 [Q15059-1] XP_006717354.1, XM_006717291.2 [Q15059-1] XP_011517354.1, XM_011519052.2 [Q15059-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2E7N | NMR | - | A | 306-415 | [»] | |
2NXB | X-ray | 1.40 | A/B | 24-144 | [»] | |
2OO1 | X-ray | 1.70 | A/B/C/D | 307-416 | [»] | |
2YW5 | NMR | - | A | 25-155 | [»] | |
3S91 | X-ray | 2.06 | A | 24-144 | [»] | |
3S92 | X-ray | 1.36 | A | 306-416 | [»] | |
5A7C | X-ray | 1.90 | A/B/C/D | 306-416 | [»] | |
5HFR | X-ray | 1.70 | A/B/C/D | 306-416 | [»] | |
5HJC | X-ray | 2.60 | A | 307-416 | [»] | |
6BGG | NMR | - | B | 557-644 | [»] | |
6BGH | NMR | - | A | 557-643 | [»] | |
6I41 | X-ray | 1.90 | B | 245-253 | [»] | |
6I5P | X-ray | 1.81 | B/D/F/H | 245-253 | [»] | |
6I68 | X-ray | 1.85 | B/D/F/H | 245-253 | [»] | |
6I7A | X-ray | 2.20 | B/D/F/H | 245-253 | [»] | |
6QJU | X-ray | 1.20 | A/B | 24-144 | [»] | |
6U4A | X-ray | 1.88 | A/B | 25-147 | [»] | |
6ULP | X-ray | 2.80 | A/B | 307-419 | [»] | |
7JMY | NMR | - | A | 554-640 | [»] | |
7JQ8 | NMR | - | A | 554-640 | [»] | |
7JYN | NMR | - | A | 554-640 | [»] | |
7JYZ | NMR | - | B | 554-640 | [»] | |
7L9L | X-ray | 1.55 | A | 306-416 | [»] | |
7LAY | X-ray | 1.45 | A/B | 24-144 | [»] | |
7LAZ | X-ray | 2.30 | A/B | 24-144 | [»] | |
7LB4 | X-ray | 2.00 | A/B | 306-416 | [»] | |
7LBT | X-ray | 2.70 | A/B/C/D | 306-416 | [»] | |
SASBDBi | Q15059 | |||||
SMRi | Q15059 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 113715, 230 interactors |
DIPi | DIP-39755N |
IntActi | Q15059, 23 interactors |
MINTi | Q15059 |
STRINGi | 9606.ENSP00000305918 |
Chemistry databases
BindingDBi | Q15059 |
ChEMBLi | CHEMBL1795186 |
GuidetoPHARMACOLOGYi | 2725 |
PTM databases
GlyGeni | Q15059, 5 sites, 1 O-linked glycan (5 sites) |
iPTMneti | Q15059 |
PhosphoSitePlusi | Q15059 |
Genetic variation databases
BioMutai | BRD3 |
DMDMi | 12643726 |
Proteomic databases
EPDi | Q15059 |
jPOSTi | Q15059 |
MassIVEi | Q15059 |
MaxQBi | Q15059 |
PaxDbi | Q15059 |
PeptideAtlasi | Q15059 |
PRIDEi | Q15059 |
ProteomicsDBi | 60414 [Q15059-1] 60415 [Q15059-2] |
Protocols and materials databases
ABCDi | Q15059, 1 sequenced antibody |
Antibodypediai | 31966, 387 antibodies from 34 providers |
DNASUi | 8019 |
Genome annotation databases
Ensembli | ENST00000303407; ENSP00000305918; ENSG00000169925 ENST00000371834; ENSP00000360900; ENSG00000169925 [Q15059-2] |
GeneIDi | 8019 |
KEGGi | hsa:8019 |
MANE-Selecti | ENST00000303407.12; ENSP00000305918.6; NM_007371.4; NP_031397.1 |
UCSCi | uc004cew.4, human [Q15059-1] |
Organism-specific databases
CTDi | 8019 |
DisGeNETi | 8019 |
GeneCardsi | BRD3 |
HGNCi | HGNC:1104, BRD3 |
HPAi | ENSG00000169925, Low tissue specificity |
MIMi | 601541, gene |
neXtProti | NX_Q15059 |
OpenTargetsi | ENSG00000169925 |
PharmGKBi | PA25415 |
VEuPathDBi | HostDB:ENSG00000169925 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1474, Eukaryota |
GeneTreei | ENSGT00940000153385 |
HOGENOMi | CLU_001499_0_4_1 |
InParanoidi | Q15059 |
OMAi | CYAFNPD |
PhylomeDBi | Q15059 |
TreeFami | TF317345 |
Enzyme and pathway databases
PathwayCommonsi | Q15059 |
SignaLinki | Q15059 |
SIGNORi | Q15059 |
Miscellaneous databases
BioGRID-ORCSi | 8019, 6 hits in 1053 CRISPR screens |
ChiTaRSi | BRD3, human |
EvolutionaryTracei | Q15059 |
GeneWikii | BRD3 |
GenomeRNAii | 8019 |
Pharosi | Q15059, Tchem |
PROi | PR:Q15059 |
RNActi | Q15059, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000169925, Expressed in forebrain and 259 other tissues |
ExpressionAtlasi | Q15059, baseline and differential |
Genevisiblei | Q15059, HS |
Family and domain databases
CDDi | cd05497, Bromo_Brdt_I_like, 1 hit cd05498, Bromo_Brdt_II_like, 1 hit |
Gene3Di | 1.20.1270.220, 1 hit 1.20.920.10, 2 hits |
InterProi | View protein in InterPro IPR043508, Bromo_Brdt_I IPR043509, Bromo_Brdt_II IPR001487, Bromodomain IPR036427, Bromodomain-like_sf IPR018359, Bromodomain_CS IPR027353, NET_dom IPR038336, NET_sf |
Pfami | View protein in Pfam PF17035, BET, 1 hit PF00439, Bromodomain, 2 hits |
PRINTSi | PR00503, BROMODOMAIN |
SMARTi | View protein in SMART SM00297, BROMO, 2 hits |
SUPFAMi | SSF47370, SSF47370, 2 hits |
PROSITEi | View protein in PROSITE PS00633, BROMODOMAIN_1, 2 hits PS50014, BROMODOMAIN_2, 2 hits PS51525, NET, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | BRD3_HUMAN | |
Accessioni | Q15059Primary (citable) accession number: Q15059 Secondary accession number(s): B1APD9 Q92645 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 11, 2001 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 194 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 9
Human chromosome 9: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references