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Protein

Bromodomain-containing protein 3

Gene

BRD3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Chromatin reader that recognizes and binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling and interaction with transcription factors (PubMed:18406326, PubMed:27105114). Regulates transcription by promoting the binding of the transcription factor GATA1 to its targets (By similarity).By similarity2 Publications

GO - Molecular functioni

  • chromatin binding Source: UniProtKB
  • lysine-acetylated histone binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

SIGNORiQ15059

Names & Taxonomyi

Protein namesi
Recommended name:
Bromodomain-containing protein 3
Alternative name(s):
RING3-like protein
Gene namesi
Name:BRD3
Synonyms:KIAA0043, RING3L
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

EuPathDBiHostDB:ENSG00000169925.16
HGNCiHGNC:1104 BRD3
MIMi601541 gene
neXtProtiNX_Q15059

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Involvement in diseasei

A chromosomal aberration involving BRD3 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;9)(q14;q34) with NUT which produces a BRD3-NUT fusion protein.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei647 – 648Breakpoint for translocation to form BDR3-NUT fusion protein1 Publication2

Organism-specific databases

DisGeNETi8019
OpenTargetsiENSG00000169925
PharmGKBiPA25415

Chemistry databases

ChEMBLiCHEMBL1795186
GuidetoPHARMACOLOGYi2725

Polymorphism and mutation databases

BioMutaiBRD3
DMDMi12643726

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002111812 – 726Bromodomain-containing protein 3Add BLAST725

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei263PhosphoserineCombined sources1
Modified residuei281PhosphoserineCombined sources1
Cross-linki414Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei563PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ15059
MaxQBiQ15059
PaxDbiQ15059
PeptideAtlasiQ15059
PRIDEiQ15059
ProteomicsDBi60414
60415 [Q15059-2]

PTM databases

iPTMnetiQ15059
PhosphoSitePlusiQ15059

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiENSG00000169925 Expressed in 248 organ(s), highest expression level in forebrain
CleanExiHS_BRD3
ExpressionAtlasiQ15059 baseline and differential
GenevisibleiQ15059 HS

Organism-specific databases

HPAiHPA051830

Interactioni

Subunit structurei

Interacts (via bromo domain 1) with GATA1 acetylated at 'Lys-312' and 'Lys-315'. Interacts (via bromo domain 1) with GATA2 acetylated on lysine residues (By similarity). Interacts (via bromo domains) with acetylated lysine residues on the N-terminus of histone H2A, H2B, H3 and H4 (in vitro).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MYCP011063EBI-1383460,EBI-447544

GO - Molecular functioni

Protein-protein interaction databases

BioGridi113715, 33 interactors
DIPiDIP-39755N
IntActiQ15059, 11 interactors
MINTiQ15059
STRINGi9606.ENSP00000305918

Chemistry databases

BindingDBiQ15059

Structurei

Secondary structure

1726
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ15059
SMRiQ15059
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ15059

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini51 – 123Bromo 1PROSITE-ProRule annotationAdd BLAST73
Domaini326 – 398Bromo 2PROSITE-ProRule annotationAdd BLAST73
Domaini562 – 644NETPROSITE-ProRule annotationAdd BLAST83

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni78 – 80Acetylated histone H3 binding1 Publication3

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili453 – 524Sequence analysisAdd BLAST72
Coiled coili645 – 684Sequence analysisAdd BLAST40

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi487 – 555Lys-richAdd BLAST69
Compositional biasi676 – 725Ser-richAdd BLAST50

Domaini

The Bromo domains specifically recognize and bind acetylated histones.1 Publication

Keywords - Domaini

Bromodomain, Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG1474 Eukaryota
COG5076 LUCA
GeneTreeiENSGT00930000150866
HOGENOMiHOG000231200
HOVERGENiHBG004896
InParanoidiQ15059
KOiK11721
OMAiEGLPMTY
OrthoDBiEOG091G01QC
PhylomeDBiQ15059
TreeFamiTF317345

Family and domain databases

Gene3Di1.20.1270.220, 1 hit
1.20.920.10, 2 hits
InterProiView protein in InterPro
IPR001487 Bromodomain
IPR036427 Bromodomain-like_sf
IPR018359 Bromodomain_CS
IPR027353 NET_dom
IPR038336 NET_sf
PfamiView protein in Pfam
PF17035 BET, 1 hit
PF00439 Bromodomain, 2 hits
PRINTSiPR00503 BROMODOMAIN
SMARTiView protein in SMART
SM00297 BROMO, 2 hits
SUPFAMiSSF47370 SSF47370, 2 hits
PROSITEiView protein in PROSITE
PS00633 BROMODOMAIN_1, 2 hits
PS50014 BROMODOMAIN_2, 2 hits
PS51525 NET, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q15059-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSTATTVAPA GIPATPGPVN PPPPEVSNPS KPGRKTNQLQ YMQNVVVKTL
60 70 80 90 100
WKHQFAWPFY QPVDAIKLNL PDYHKIIKNP MDMGTIKKRL ENNYYWSASE
110 120 130 140 150
CMQDFNTMFT NCYIYNKPTD DIVLMAQALE KIFLQKVAQM PQEEVELLPP
160 170 180 190 200
APKGKGRKPA AGAQSAGTQQ VAAVSSVSPA TPFQSVPPTV SQTPVIAATP
210 220 230 240 250
VPTITANVTS VPVPPAAAPP PPATPIVPVV PPTPPVVKKK GVKRKADTTT
260 270 280 290 300
PTTSAITASR SESPPPLSDP KQAKVVARRE SGGRPIKPPK KDLEDGEVPQ
310 320 330 340 350
HAGKKGKLSE HLRYCDSILR EMLSKKHAAY AWPFYKPVDA EALELHDYHD
360 370 380 390 400
IIKHPMDLST VKRKMDGREY PDAQGFAADV RLMFSNCYKY NPPDHEVVAM
410 420 430 440 450
ARKLQDVFEM RFAKMPDEPV EAPALPAPAA PMVSKGAESS RSSEESSSDS
460 470 480 490 500
GSSDSEEERA TRLAELQEQL KAVHEQLAAL SQAPVNKPKK KKEKKEKEKK
510 520 530 540 550
KKDKEKEKEK HKVKAEEEKK AKVAPPAKQA QQKKAPAKKA NSTTTAGRQL
560 570 580 590 600
KKGGKQASAS YDSEEEEEGL PMSYDEKRQL SLDINRLPGE KLGRVVHIIQ
610 620 630 640 650
SREPSLRDSN PDEIEIDFET LKPTTLRELE RYVKSCLQKK QRKPFSASGK
660 670 680 690 700
KQAAKSKEEL AQEKKKELEK RLQDVSGQLS SSKKPARKEK PGSAPSGGPS
710 720
RLSSSSSSES GSSSSSGSSS DSSDSE
Length:726
Mass (Da):79,542
Last modified:November 1, 1996 - v1
Checksum:i64F526FC3C1033AA
GO
Isoform 2 (identifier: Q15059-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     549-556: QLKKGGKQ → DHFLTCGV
     557-726: Missing.

Note: No experimental confirmation available.
Show »
Length:556
Mass (Da):60,942
Checksum:i8352F5DF1801A793
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A9J571A9J571_HUMAN
Bromodomain-containing protein 3
BRD3
74Annotation score:
A9J575A9J575_HUMAN
Bromodomain-containing protein 3
BRD3
20Annotation score:

Sequence cautioni

The sequence BAA05393 differs from that shown. Reason: Erroneous initiation.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04191336T → N in a renal clear cell carcinoma sample; somatic mutation. 1 Publication1
Natural variantiVAR_041914161A → T in a gastric adenocarcinoma sample; somatic mutation. 1 Publication1
Natural variantiVAR_041915172A → V1 PublicationCorresponds to variant dbSNP:rs34609592Ensembl.1
Natural variantiVAR_041916435K → Q1 PublicationCorresponds to variant dbSNP:rs36093130Ensembl.1
Natural variantiVAR_041917441R → H1 PublicationCorresponds to variant dbSNP:rs56017928Ensembl.1
Natural variantiVAR_041918447S → P1 PublicationCorresponds to variant dbSNP:rs55754444Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_010247549 – 556QLKKGGKQ → DHFLTCGV in isoform 2. 1 Publication8
Alternative sequenceiVSP_010248557 – 726Missing in isoform 2. 1 PublicationAdd BLAST170

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26362 mRNA Translation: BAA05393.2 Different initiation.
AY513270 mRNA Translation: AAS82952.1
AL445931 Genomic DNA No translation available.
CH471090 Genomic DNA Translation: EAW88113.1
CH471090 Genomic DNA Translation: EAW88114.1
BC032124 mRNA Translation: AAH32124.1
Z81330 Genomic DNA No translation available.
CCDSiCCDS6980.1 [Q15059-1]
RefSeqiNP_031397.1, NM_007371.3 [Q15059-1]
XP_006717354.1, XM_006717291.2 [Q15059-1]
XP_011517354.1, XM_011519052.2 [Q15059-1]
UniGeneiHs.522472
Hs.654869

Genome annotation databases

EnsembliENST00000303407; ENSP00000305918; ENSG00000169925 [Q15059-1]
ENST00000371834; ENSP00000360900; ENSG00000169925 [Q15059-2]
GeneIDi8019
KEGGihsa:8019
UCSCiuc004cew.4 human [Q15059-1]

Keywords - Coding sequence diversityi

Alternative splicing, Chromosomal rearrangement, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26362 mRNA Translation: BAA05393.2 Different initiation.
AY513270 mRNA Translation: AAS82952.1
AL445931 Genomic DNA No translation available.
CH471090 Genomic DNA Translation: EAW88113.1
CH471090 Genomic DNA Translation: EAW88114.1
BC032124 mRNA Translation: AAH32124.1
Z81330 Genomic DNA No translation available.
CCDSiCCDS6980.1 [Q15059-1]
RefSeqiNP_031397.1, NM_007371.3 [Q15059-1]
XP_006717354.1, XM_006717291.2 [Q15059-1]
XP_011517354.1, XM_011519052.2 [Q15059-1]
UniGeneiHs.522472
Hs.654869

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2E7NNMR-A306-415[»]
2NXBX-ray1.40A/B24-144[»]
2OO1X-ray1.70A/B/C/D307-416[»]
2YW5NMR-A25-155[»]
3S91X-ray2.06A24-144[»]
3S92X-ray1.36A306-416[»]
5A7CX-ray1.90A/B/C/D306-416[»]
5HFRX-ray1.70A/B/C/D306-416[»]
5HJCX-ray2.60A307-416[»]
6BGGNMR-B557-644[»]
6BGHNMR-A557-643[»]
ProteinModelPortaliQ15059
SMRiQ15059
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113715, 33 interactors
DIPiDIP-39755N
IntActiQ15059, 11 interactors
MINTiQ15059
STRINGi9606.ENSP00000305918

Chemistry databases

BindingDBiQ15059
ChEMBLiCHEMBL1795186
GuidetoPHARMACOLOGYi2725

PTM databases

iPTMnetiQ15059
PhosphoSitePlusiQ15059

Polymorphism and mutation databases

BioMutaiBRD3
DMDMi12643726

Proteomic databases

EPDiQ15059
MaxQBiQ15059
PaxDbiQ15059
PeptideAtlasiQ15059
PRIDEiQ15059
ProteomicsDBi60414
60415 [Q15059-2]

Protocols and materials databases

DNASUi8019
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000303407; ENSP00000305918; ENSG00000169925 [Q15059-1]
ENST00000371834; ENSP00000360900; ENSG00000169925 [Q15059-2]
GeneIDi8019
KEGGihsa:8019
UCSCiuc004cew.4 human [Q15059-1]

Organism-specific databases

CTDi8019
DisGeNETi8019
EuPathDBiHostDB:ENSG00000169925.16
GeneCardsiBRD3
HGNCiHGNC:1104 BRD3
HPAiHPA051830
MIMi601541 gene
neXtProtiNX_Q15059
OpenTargetsiENSG00000169925
PharmGKBiPA25415
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1474 Eukaryota
COG5076 LUCA
GeneTreeiENSGT00930000150866
HOGENOMiHOG000231200
HOVERGENiHBG004896
InParanoidiQ15059
KOiK11721
OMAiEGLPMTY
OrthoDBiEOG091G01QC
PhylomeDBiQ15059
TreeFamiTF317345

Enzyme and pathway databases

SIGNORiQ15059

Miscellaneous databases

ChiTaRSiBRD3 human
EvolutionaryTraceiQ15059
GeneWikiiBRD3
GenomeRNAii8019
PROiPR:Q15059
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000169925 Expressed in 248 organ(s), highest expression level in forebrain
CleanExiHS_BRD3
ExpressionAtlasiQ15059 baseline and differential
GenevisibleiQ15059 HS

Family and domain databases

Gene3Di1.20.1270.220, 1 hit
1.20.920.10, 2 hits
InterProiView protein in InterPro
IPR001487 Bromodomain
IPR036427 Bromodomain-like_sf
IPR018359 Bromodomain_CS
IPR027353 NET_dom
IPR038336 NET_sf
PfamiView protein in Pfam
PF17035 BET, 1 hit
PF00439 Bromodomain, 2 hits
PRINTSiPR00503 BROMODOMAIN
SMARTiView protein in SMART
SM00297 BROMO, 2 hits
SUPFAMiSSF47370 SSF47370, 2 hits
PROSITEiView protein in PROSITE
PS00633 BROMODOMAIN_1, 2 hits
PS50014 BROMODOMAIN_2, 2 hits
PS51525 NET, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiBRD3_HUMAN
AccessioniPrimary (citable) accession number: Q15059
Secondary accession number(s): B1APD9
, Q4G5Y3, Q5T1R7, Q8N5M3, Q92645
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: November 1, 1996
Last modified: November 7, 2018
This is version 172 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  2. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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