UniProtKB - Q15035 (TRAM2_HUMAN)
Protein
Translocating chain-associated membrane protein 2
Gene
TRAM2
Organism
Homo sapiens (Human)
Status
Functioni
Necessary for collagen type I synthesis. May couple the activity of the ER Ca2+ pump SERCA2B with the activity of the translocon. This coupling may increase the local Ca2+ concentration at the site of collagen synthesis, and a high Ca2+ concentration may be necessary for the function of molecular chaperones involved in collagen folding. Required for proper insertion of the first transmembrane helix N-terminus of TM4SF20 into the ER lumen, may act as a ceramide sensor for regulated alternative translocation (RAT) (PubMed:27499293).2 Publications
GO - Biological processi
- collagen biosynthetic process Source: UniProtKB
- protein insertion into ER membrane Source: UniProtKB
- SRP-dependent cotranslational protein targeting to membrane, translocation Source: InterPro
Keywordsi
Biological process | Protein transport, Translocation, Transport |
Enzyme and pathway databases
PathwayCommonsi | Q15035 |
Protein family/group databases
TCDBi | 9.B.311.2.4, the 6-7 tms tram-lag (tram-lag) family |
Names & Taxonomyi
Protein namesi | Recommended name: Translocating chain-associated membrane protein 2 |
Gene namesi | Name:TRAM2 Synonyms:KIAA0057 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:16855, TRAM2 |
MIMi | 608485, gene |
neXtProti | NX_Q15035 |
VEuPathDBi | HostDB:ENSG00000065308.4 |
Subcellular locationi
Other locations
- Membrane Curated; Multi-pass membrane protein Sequence analysis
Endoplasmic reticulum
- endoplasmic reticulum Source: GO_Central
- integral component of endoplasmic reticulum membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 22 | CytoplasmicCuratedAdd BLAST | 22 | |
Transmembranei | 23 – 43 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 44 – 75 | ExtracellularCuratedAdd BLAST | 32 | |
Transmembranei | 76 – 96 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 97 – 119 | CytoplasmicCuratedAdd BLAST | 23 | |
Transmembranei | 120 – 140 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 141 – 159 | ExtracellularCuratedAdd BLAST | 19 | |
Transmembranei | 160 – 180 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 181 – 191 | CytoplasmicCuratedAdd BLAST | 11 | |
Transmembranei | 192 – 209 | HelicalSequence analysisAdd BLAST | 18 | |
Topological domaini | 210 – 214 | ExtracellularCurated | 5 | |
Transmembranei | 215 – 235 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 236 – 250 | CytoplasmicCuratedAdd BLAST | 15 | |
Transmembranei | 251 – 271 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 272 – 287 | ExtracellularCuratedAdd BLAST | 16 | |
Transmembranei | 288 – 308 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 309 – 370 | CytoplasmicCuratedAdd BLAST | 62 |
Keywords - Cellular componenti
MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 9697 |
OpenTargetsi | ENSG00000065308 |
PharmGKBi | PA128394555 |
Miscellaneous databases
Pharosi | Q15035, Tbio |
Genetic variation databases
BioMutai | TRAM2 |
DMDMi | 18202502 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000185532 | 1 – 370 | Translocating chain-associated membrane protein 2Add BLAST | 370 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 55 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
EPDi | Q15035 |
jPOSTi | Q15035 |
MassIVEi | Q15035 |
MaxQBi | Q15035 |
PaxDbi | Q15035 |
PeptideAtlasi | Q15035 |
PRIDEi | Q15035 |
ProteomicsDBi | 60384 |
PTM databases
GlyGeni | Q15035, 1 site |
iPTMneti | Q15035 |
PhosphoSitePlusi | Q15035 |
SwissPalmi | Q15035 |
Expressioni
Gene expression databases
Bgeei | ENSG00000065308, Expressed in oocyte and 214 other tissues |
ExpressionAtlasi | Q15035, baseline and differential |
Genevisiblei | Q15035, HS |
Organism-specific databases
HPAi | ENSG00000065308, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with SERCA2B and COL1A1.
1 PublicationProtein-protein interaction databases
BioGRIDi | 115049, 6 interactors |
IntActi | Q15035, 1 interactor |
STRINGi | 9606.ENSP00000182527 |
Miscellaneous databases
RNActi | Q15035, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 112 – 321 | TLCPROSITE-ProRule annotationAdd BLAST | 210 |
Sequence similaritiesi
Belongs to the TRAM family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1608, Eukaryota |
GeneTreei | ENSGT00510000046470 |
HOGENOMi | CLU_062830_0_0_1 |
InParanoidi | Q15035 |
OMAi | CRLCMLL |
OrthoDBi | 831082at2759 |
PhylomeDBi | Q15035 |
TreeFami | TF314319 |
Family and domain databases
InterProi | View protein in InterPro IPR006634, TLC-dom IPR013599, TRAM1 IPR016447, Translocation_assoc_membrane |
PANTHERi | PTHR12371, PTHR12371, 1 hit |
Pfami | View protein in Pfam PF08390, TRAM1, 1 hit PF03798, TRAM_LAG1_CLN8, 1 hit |
PIRSFi | PIRSF005449, Translocation_assoc_membrane, 1 hit |
SMARTi | View protein in SMART SM00724, TLC, 1 hit |
PROSITEi | View protein in PROSITE PS50922, TLC, 1 hit |
i Sequence
Sequence statusi: Complete.
Q15035-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAFRRRTKSY PLFSQEFVIH NHADIGFCLV LCVLIGLMFE VTAKTAFLFI
60 70 80 90 100
LPQYNISVPT ADSETVHYHY GPKDLVTILF YIFITIILHA VVQEYILDKI
110 120 130 140 150
SKRLHLSKVK HSKFNESGQL VVFHFTSVIW CFYVVVTEGY LTNPRSLWED
160 170 180 190 200
YPHVHLPFQV KFFYLCQLAY WLHALPELYF QKVRKEEIPR QLQYICLYLV
210 220 230 240 250
HIAGAYLLNL SRLGLILLLL QYSTEFLFHT ARLFYFADEN NEKLFSAWAA
260 270 280 290 300
VFGVTRLFIL TLAVLAIGFG LARMENQAFD PEKGNFNTLF CRLCVLLLVC
310 320 330 340 350
AAQAWLMWRF IHSQLRHWRE YWNEQSAKRR VPATPRLPAR LIKRESGYHE
360 370
NGVVKAENGT SPRTKKLKSP
Sequence cautioni
The sequence BAA06540 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D31762 mRNA Translation: BAA06540.2 Different initiation. AK291751 mRNA Translation: BAF84440.1 AL049611 Genomic DNA No translation available. CH471081 Genomic DNA Translation: EAX04373.1 BC028121 mRNA Translation: AAH28121.1 |
CCDSi | CCDS34477.1 |
RefSeqi | NP_036420.1, NM_012288.3 |
Genome annotation databases
Ensembli | ENST00000182527; ENSP00000182527; ENSG00000065308 |
GeneIDi | 9697 |
KEGGi | hsa:9697 |
UCSCi | uc003paq.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D31762 mRNA Translation: BAA06540.2 Different initiation. AK291751 mRNA Translation: BAF84440.1 AL049611 Genomic DNA No translation available. CH471081 Genomic DNA Translation: EAX04373.1 BC028121 mRNA Translation: AAH28121.1 |
CCDSi | CCDS34477.1 |
RefSeqi | NP_036420.1, NM_012288.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 115049, 6 interactors |
IntActi | Q15035, 1 interactor |
STRINGi | 9606.ENSP00000182527 |
Protein family/group databases
TCDBi | 9.B.311.2.4, the 6-7 tms tram-lag (tram-lag) family |
PTM databases
GlyGeni | Q15035, 1 site |
iPTMneti | Q15035 |
PhosphoSitePlusi | Q15035 |
SwissPalmi | Q15035 |
Genetic variation databases
BioMutai | TRAM2 |
DMDMi | 18202502 |
Proteomic databases
EPDi | Q15035 |
jPOSTi | Q15035 |
MassIVEi | Q15035 |
MaxQBi | Q15035 |
PaxDbi | Q15035 |
PeptideAtlasi | Q15035 |
PRIDEi | Q15035 |
ProteomicsDBi | 60384 |
Protocols and materials databases
Antibodypediai | 30914, 163 antibodies |
DNASUi | 9697 |
Genome annotation databases
Ensembli | ENST00000182527; ENSP00000182527; ENSG00000065308 |
GeneIDi | 9697 |
KEGGi | hsa:9697 |
UCSCi | uc003paq.4, human |
Organism-specific databases
CTDi | 9697 |
DisGeNETi | 9697 |
GeneCardsi | TRAM2 |
HGNCi | HGNC:16855, TRAM2 |
HPAi | ENSG00000065308, Low tissue specificity |
MIMi | 608485, gene |
neXtProti | NX_Q15035 |
OpenTargetsi | ENSG00000065308 |
PharmGKBi | PA128394555 |
VEuPathDBi | HostDB:ENSG00000065308.4 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1608, Eukaryota |
GeneTreei | ENSGT00510000046470 |
HOGENOMi | CLU_062830_0_0_1 |
InParanoidi | Q15035 |
OMAi | CRLCMLL |
OrthoDBi | 831082at2759 |
PhylomeDBi | Q15035 |
TreeFami | TF314319 |
Enzyme and pathway databases
PathwayCommonsi | Q15035 |
Miscellaneous databases
BioGRID-ORCSi | 9697, 17 hits in 873 CRISPR screens |
ChiTaRSi | TRAM2, human |
GeneWikii | TRAM2 |
GenomeRNAii | 9697 |
Pharosi | Q15035, Tbio |
PROi | PR:Q15035 |
RNActi | Q15035, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000065308, Expressed in oocyte and 214 other tissues |
ExpressionAtlasi | Q15035, baseline and differential |
Genevisiblei | Q15035, HS |
Family and domain databases
InterProi | View protein in InterPro IPR006634, TLC-dom IPR013599, TRAM1 IPR016447, Translocation_assoc_membrane |
PANTHERi | PTHR12371, PTHR12371, 1 hit |
Pfami | View protein in Pfam PF08390, TRAM1, 1 hit PF03798, TRAM_LAG1_CLN8, 1 hit |
PIRSFi | PIRSF005449, Translocation_assoc_membrane, 1 hit |
SMARTi | View protein in SMART SM00724, TLC, 1 hit |
PROSITEi | View protein in PROSITE PS50922, TLC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TRAM2_HUMAN | |
Accessioni | Q15035Primary (citable) accession number: Q15035 Secondary accession number(s): A8K6T6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 29, 2001 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 10, 2021 | |
This is version 152 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 6
Human chromosome 6: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families