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Protein

Translocating chain-associated membrane protein 2

Gene

TRAM2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Necessary for collagen type I synthesis. May couple the activity of the ER Ca2+ pump SERCA2B with the activity of the translocon. This coupling may increase the local Ca2+ concentration at the site of collagen synthesis, and a high Ca2+ concentration may be necessary for the function of molecular chaperones involved in collagen folding. Required for proper insertion of the first transmembrane helix N-terminus of TM4SF20 into the ER lumen, may act as a ceramide sensor for regulated alternative translocation (RAT) (PubMed:27499293).2 Publications

GO - Biological processi

  • collagen biosynthetic process Source: UniProtKB
  • protein insertion into ER membrane Source: UniProtKB
  • SRP-dependent cotranslational protein targeting to membrane, translocation Source: InterPro

Keywordsi

Biological processProtein transport, Translocation, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Translocating chain-associated membrane protein 2
Gene namesi
Name:TRAM2
Synonyms:KIAA0057
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000065308.4
HGNCiHGNC:16855 TRAM2
MIMi608485 gene
neXtProtiNX_Q15035

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 22CytoplasmicCuratedAdd BLAST22
Transmembranei23 – 43HelicalSequence analysisAdd BLAST21
Topological domaini44 – 75ExtracellularCuratedAdd BLAST32
Transmembranei76 – 96HelicalSequence analysisAdd BLAST21
Topological domaini97 – 119CytoplasmicCuratedAdd BLAST23
Transmembranei120 – 140HelicalSequence analysisAdd BLAST21
Topological domaini141 – 159ExtracellularCuratedAdd BLAST19
Transmembranei160 – 180HelicalSequence analysisAdd BLAST21
Topological domaini181 – 191CytoplasmicCuratedAdd BLAST11
Transmembranei192 – 209HelicalSequence analysisAdd BLAST18
Topological domaini210 – 214ExtracellularCurated5
Transmembranei215 – 235HelicalSequence analysisAdd BLAST21
Topological domaini236 – 250CytoplasmicCuratedAdd BLAST15
Transmembranei251 – 271HelicalSequence analysisAdd BLAST21
Topological domaini272 – 287ExtracellularCuratedAdd BLAST16
Transmembranei288 – 308HelicalSequence analysisAdd BLAST21
Topological domaini309 – 370CytoplasmicCuratedAdd BLAST62

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi9697
OpenTargetsiENSG00000065308
PharmGKBiPA128394555

Polymorphism and mutation databases

BioMutaiTRAM2
DMDMi18202502

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001855321 – 370Translocating chain-associated membrane protein 2Add BLAST370

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi55N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ15035
MaxQBiQ15035
PaxDbiQ15035
PeptideAtlasiQ15035
PRIDEiQ15035
ProteomicsDBi60384

PTM databases

iPTMnetiQ15035
PhosphoSitePlusiQ15035
SwissPalmiQ15035

Expressioni

Gene expression databases

BgeeiENSG00000065308 Expressed in 204 organ(s), highest expression level in oocyte
CleanExiHS_TRAM2
ExpressionAtlasiQ15035 baseline and differential
GenevisibleiQ15035 HS

Organism-specific databases

HPAiHPA035092
HPA057925

Interactioni

Subunit structurei

Interacts with SERCA2B and COL1A1.1 Publication

Protein-protein interaction databases

BioGridi115049, 2 interactors
STRINGi9606.ENSP00000182527

Structurei

3D structure databases

ProteinModelPortaliQ15035
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini112 – 321TLCPROSITE-ProRule annotationAdd BLAST210

Sequence similaritiesi

Belongs to the TRAM family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1608 Eukaryota
ENOG410XSB3 LUCA
GeneTreeiENSGT00510000046470
HOGENOMiHOG000007494
HOVERGENiHBG054402
InParanoidiQ15035
OMAiCLYVVVT
OrthoDBiEOG091G077N
PhylomeDBiQ15035
TreeFamiTF314319

Family and domain databases

InterProiView protein in InterPro
IPR006634 TLC-dom
IPR013599 TRAM1
IPR016447 Translocation_assoc_membrane
PANTHERiPTHR12371 PTHR12371, 1 hit
PfamiView protein in Pfam
PF08390 TRAM1, 1 hit
PF03798 TRAM_LAG1_CLN8, 1 hit
PIRSFiPIRSF005449 Translocation_assoc_membrane, 1 hit
SMARTiView protein in SMART
SM00724 TLC, 1 hit
PROSITEiView protein in PROSITE
PS50922 TLC, 1 hit

Sequencei

Sequence statusi: Complete.

Q15035-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAFRRRTKSY PLFSQEFVIH NHADIGFCLV LCVLIGLMFE VTAKTAFLFI
60 70 80 90 100
LPQYNISVPT ADSETVHYHY GPKDLVTILF YIFITIILHA VVQEYILDKI
110 120 130 140 150
SKRLHLSKVK HSKFNESGQL VVFHFTSVIW CFYVVVTEGY LTNPRSLWED
160 170 180 190 200
YPHVHLPFQV KFFYLCQLAY WLHALPELYF QKVRKEEIPR QLQYICLYLV
210 220 230 240 250
HIAGAYLLNL SRLGLILLLL QYSTEFLFHT ARLFYFADEN NEKLFSAWAA
260 270 280 290 300
VFGVTRLFIL TLAVLAIGFG LARMENQAFD PEKGNFNTLF CRLCVLLLVC
310 320 330 340 350
AAQAWLMWRF IHSQLRHWRE YWNEQSAKRR VPATPRLPAR LIKRESGYHE
360 370
NGVVKAENGT SPRTKKLKSP
Length:370
Mass (Da):43,328
Last modified:November 1, 1996 - v1
Checksum:i9B5183F1A3D45366
GO

Sequence cautioni

The sequence BAA06540 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D31762 mRNA Translation: BAA06540.2 Different initiation.
AK291751 mRNA Translation: BAF84440.1
AL049611 Genomic DNA No translation available.
CH471081 Genomic DNA Translation: EAX04373.1
BC028121 mRNA Translation: AAH28121.1
CCDSiCCDS34477.1
RefSeqiNP_036420.1, NM_012288.3
UniGeneiHs.520182

Genome annotation databases

EnsembliENST00000182527; ENSP00000182527; ENSG00000065308
GeneIDi9697
KEGGihsa:9697
UCSCiuc003paq.4 human

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D31762 mRNA Translation: BAA06540.2 Different initiation.
AK291751 mRNA Translation: BAF84440.1
AL049611 Genomic DNA No translation available.
CH471081 Genomic DNA Translation: EAX04373.1
BC028121 mRNA Translation: AAH28121.1
CCDSiCCDS34477.1
RefSeqiNP_036420.1, NM_012288.3
UniGeneiHs.520182

3D structure databases

ProteinModelPortaliQ15035
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115049, 2 interactors
STRINGi9606.ENSP00000182527

PTM databases

iPTMnetiQ15035
PhosphoSitePlusiQ15035
SwissPalmiQ15035

Polymorphism and mutation databases

BioMutaiTRAM2
DMDMi18202502

Proteomic databases

EPDiQ15035
MaxQBiQ15035
PaxDbiQ15035
PeptideAtlasiQ15035
PRIDEiQ15035
ProteomicsDBi60384

Protocols and materials databases

DNASUi9697
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000182527; ENSP00000182527; ENSG00000065308
GeneIDi9697
KEGGihsa:9697
UCSCiuc003paq.4 human

Organism-specific databases

CTDi9697
DisGeNETi9697
EuPathDBiHostDB:ENSG00000065308.4
GeneCardsiTRAM2
HGNCiHGNC:16855 TRAM2
HPAiHPA035092
HPA057925
MIMi608485 gene
neXtProtiNX_Q15035
OpenTargetsiENSG00000065308
PharmGKBiPA128394555
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1608 Eukaryota
ENOG410XSB3 LUCA
GeneTreeiENSGT00510000046470
HOGENOMiHOG000007494
HOVERGENiHBG054402
InParanoidiQ15035
OMAiCLYVVVT
OrthoDBiEOG091G077N
PhylomeDBiQ15035
TreeFamiTF314319

Miscellaneous databases

ChiTaRSiTRAM2 human
GeneWikiiTRAM2
GenomeRNAii9697
PROiPR:Q15035
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000065308 Expressed in 204 organ(s), highest expression level in oocyte
CleanExiHS_TRAM2
ExpressionAtlasiQ15035 baseline and differential
GenevisibleiQ15035 HS

Family and domain databases

InterProiView protein in InterPro
IPR006634 TLC-dom
IPR013599 TRAM1
IPR016447 Translocation_assoc_membrane
PANTHERiPTHR12371 PTHR12371, 1 hit
PfamiView protein in Pfam
PF08390 TRAM1, 1 hit
PF03798 TRAM_LAG1_CLN8, 1 hit
PIRSFiPIRSF005449 Translocation_assoc_membrane, 1 hit
SMARTiView protein in SMART
SM00724 TLC, 1 hit
PROSITEiView protein in PROSITE
PS50922 TLC, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTRAM2_HUMAN
AccessioniPrimary (citable) accession number: Q15035
Secondary accession number(s): A8K6T6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: November 1, 1996
Last modified: November 7, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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