TRAM2

Functionⁱ

Necessary for collagen type I synthesis. May couple the activity of the ER Ca²⁺ pump SERCA2B with the activity of the translocon. This coupling may increase the local Ca²⁺ concentration at the site of collagen synthesis, and a high Ca²⁺ concentration may be necessary for the function of molecular chaperones involved in collagen folding. Required for proper insertion of the first transmembrane helix N-terminus of TM4SF20 into the ER lumen, may act as a ceramide sensor for regulated alternative translocation (RAT) (PubMed:27499293).2 Publications

GO - Biological processⁱ

collagen biosynthetic process
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 14749390
protein insertion into ER membrane
Source: UniProtKB
Inferred from direct assayⁱ
- 27499293
SRP-dependent cotranslational protein targeting to membrane, translocation Source: InterPro

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Protein transport, Translocation, Transport

Biological process

Protein transport, Translocation, Transport

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Translocating chain-associated membrane protein 2
Gene namesⁱ	Name:TRAM2 Synonyms:KIAA0057
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 6

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000065308.4
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:16855 TRAM2
MIMⁱ	608485 gene
neXtProtⁱ	NX_Q15035

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 22	CytoplasmicCuratedAdd BLAST	22
Transmembraneⁱ	23 – 43	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	44 – 75	ExtracellularCuratedAdd BLAST	32
Transmembraneⁱ	76 – 96	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	97 – 119	CytoplasmicCuratedAdd BLAST	23
Transmembraneⁱ	120 – 140	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	141 – 159	ExtracellularCuratedAdd BLAST	19
Transmembraneⁱ	160 – 180	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	181 – 191	CytoplasmicCuratedAdd BLAST	11
Transmembraneⁱ	192 – 209	HelicalSequence analysisAdd BLAST	18
Topological domainⁱ	210 – 214	ExtracellularCurated	5
Transmembraneⁱ	215 – 235	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	236 – 250	CytoplasmicCuratedAdd BLAST	15
Transmembraneⁱ	251 – 271	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	272 – 287	ExtracellularCuratedAdd BLAST	16
Transmembraneⁱ	288 – 308	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	309 – 370	CytoplasmicCuratedAdd BLAST	62

Keywords - Cellular componentⁱ

Membrane

Pathology & Biotechⁱ

Organism-specific databases

DisGeNET More...DisGeNETⁱ	9697
Open Targets More...OpenTargetsⁱ	ENSG00000065308
PharmGKBⁱ	PA128394555

Polymorphism and mutation databases

BioMutaⁱ	TRAM2
DMDMⁱ	18202502

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000185532	1 – 370	Translocating chain-associated membrane protein 2Add BLAST		370

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Glycosylationⁱ	55	N-linked (GlcNAc...) asparagineSequence analysis		1

Keywords - PTMⁱ

Glycoprotein

Proteomic databases

EPDⁱ	Q15035
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q15035
PaxDbⁱ	Q15035
PeptideAtlas More...PeptideAtlasⁱ	Q15035
PRIDEⁱ	Q15035
ProteomicsDBⁱ	60384

PTM databases

iPTMnetⁱ	Q15035
PhosphoSitePlusⁱ	Q15035
SwissPalmⁱ	Q15035

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSG00000065308 Expressed in 204 organ(s), highest expression level in oocyte
CleanExⁱ	HS_TRAM2
ExpressionAtlasⁱ	Q15035 baseline and differential
Genevisibleⁱ	Q15035 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA035092 HPA057925

HPAⁱ

HPA035092
HPA057925

Interactionⁱ

Subunit structureⁱ

Interacts with SERCA2B and COL1A1.1 Publication

Protein-protein interaction databases

BioGridⁱ	115049, 2 interactors
STRINGⁱ	9606.ENSP00000182527

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q15035
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	112 – 321	TLCPROSITE-ProRule annotationAdd BLAST		210

Sequence similaritiesⁱ

Belongs to the TRAM family.Curated

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	KOG1608 Eukaryota ENOG410XSB3 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00510000046470
HOGENOMⁱ	HOG000007494
HOVERGENⁱ	HBG054402
InParanoidⁱ	Q15035
OMAⁱ	CLYVVVT
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G077N
PhylomeDBⁱ	Q15035
TreeFamⁱ	TF314319

Family and domain databases

InterProⁱ	View protein in InterPro IPR006634 TLC-dom IPR013599 TRAM1 IPR016447 Translocation_assoc_membrane
PANTHERⁱ	PTHR12371 PTHR12371, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF08390 TRAM1, 1 hit PF03798 TRAM_LAG1_CLN8, 1 hit
PIRSFⁱ	PIRSF005449 Translocation_assoc_membrane, 1 hit
SMARTⁱ	View protein in SMART SM00724 TLC, 1 hit
PROSITEⁱ	View protein in PROSITE PS50922 TLC, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q15035-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MAFRRRTKSY PLFSQEFVIH NHADIGFCLV LCVLIGLMFE VTAKTAFLFI 
        60         70         80         90        100
LPQYNISVPT ADSETVHYHY GPKDLVTILF YIFITIILHA VVQEYILDKI 
       110        120        130        140        150
SKRLHLSKVK HSKFNESGQL VVFHFTSVIW CFYVVVTEGY LTNPRSLWED 
       160        170        180        190        200
YPHVHLPFQV KFFYLCQLAY WLHALPELYF QKVRKEEIPR QLQYICLYLV 
       210        220        230        240        250
HIAGAYLLNL SRLGLILLLL QYSTEFLFHT ARLFYFADEN NEKLFSAWAA 
       260        270        280        290        300
VFGVTRLFIL TLAVLAIGFG LARMENQAFD PEKGNFNTLF CRLCVLLLVC 
       310        320        330        340        350
AAQAWLMWRF IHSQLRHWRE YWNEQSAKRR VPATPRLPAR LIKRESGYHE 
       360        370
NGVVKAENGT SPRTKKLKSP

Length:370

Mass (Da):43,328

Last modified:November 1, 1996 - v1

Checksum:ⁱ9B5183F1A3D45366

GO

Sequence cautionⁱ

The sequence BAA06540 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	D31762 mRNA Translation: BAA06540.2 Different initiation. AK291751 mRNA Translation: BAF84440.1 AL049611 Genomic DNA No translation available. CH471081 Genomic DNA Translation: EAX04373.1 BC028121 mRNA Translation: AAH28121.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS34477.1
NCBI Reference Sequences More...RefSeqⁱ	NP_036420.1, NM_012288.3
UniGeneⁱ	Hs.520182

Genome annotation databases

Ensemblⁱ	ENST00000182527; ENSP00000182527; ENSG00000065308
GeneIDⁱ	9697
KEGGⁱ	hsa:9697
UCSC genome browser More...UCSCⁱ	uc003paq.4 human

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q15035	Translocating chain-associated membrane protein		HUMAN		370	UniRef100_Q15035
Translocating chain-associated membrane protein		MACMU		370
Translocating chain-associated membrane protein		CERAT		370
Translocating chain-associated membrane protein		PAPAN		370
Translocating chain-associated membrane protein		CHLSB		370
+5

Protein	Similar proteins		Species	Score	Length	Source
Q15035	Translocating chain-associated membrane protein		HUMAN		370	UniRef90_Q15035
Translocating chain-associated membrane protein		MACMU		370
Translocating chain-associated membrane protein		CERAT		370
Translocating chain-associated membrane protein		PAPAN		370
Translocating chain-associated membrane protein		CHLSB		370
+20

Protein	Similar proteins		Species	Score	Length	Source
Q15035	Translocating chain-associated membrane protein 2		MOUSE		370	UniRef50_Q15035
Translocating chain-associated membrane protein		HUMAN		370
Translocating chain-associated membrane protein		MACMU		370
Translocating chain-associated membrane protein		CERAT		370
Translocating chain-associated membrane protein		PAPAN		370
+188

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	D31762 mRNA Translation: BAA06540.2 Different initiation. AK291751 mRNA Translation: BAF84440.1 AL049611 Genomic DNA No translation available. CH471081 Genomic DNA Translation: EAX04373.1 BC028121 mRNA Translation: AAH28121.1
CCDSⁱ	CCDS34477.1
RefSeqⁱ	NP_036420.1, NM_012288.3
UniGeneⁱ	Hs.520182

3D structure databases

ProteinModelPortalⁱ	Q15035
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	115049, 2 interactors
STRINGⁱ	9606.ENSP00000182527

PTM databases

iPTMnetⁱ	Q15035
PhosphoSitePlusⁱ	Q15035
SwissPalmⁱ	Q15035

Polymorphism and mutation databases

BioMutaⁱ	TRAM2
DMDMⁱ	18202502

Proteomic databases

EPDⁱ	Q15035
MaxQBⁱ	Q15035
PaxDbⁱ	Q15035
PeptideAtlasⁱ	Q15035
PRIDEⁱ	Q15035
ProteomicsDBⁱ	60384

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	9697
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000182527; ENSP00000182527; ENSG00000065308
GeneIDⁱ	9697
KEGGⁱ	hsa:9697
UCSCⁱ	uc003paq.4 human

Organism-specific databases

CTDⁱ	9697
DisGeNETⁱ	9697
EuPathDBⁱ	HostDB:ENSG00000065308.4
GeneCardsⁱ	TRAM2
HGNCⁱ	HGNC:16855 TRAM2
HPAⁱ	HPA035092 HPA057925
MIMⁱ	608485 gene
neXtProtⁱ	NX_Q15035
OpenTargetsⁱ	ENSG00000065308
PharmGKBⁱ	PA128394555
HUGEⁱ	Search...
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG1608 Eukaryota ENOG410XSB3 LUCA
GeneTreeⁱ	ENSGT00510000046470
HOGENOMⁱ	HOG000007494
HOVERGENⁱ	HBG054402
InParanoidⁱ	Q15035
OMAⁱ	CLYVVVT
OrthoDBⁱ	EOG091G077N
PhylomeDBⁱ	Q15035
TreeFamⁱ	TF314319

Miscellaneous databases

ChiTaRSⁱ	TRAM2 human
GeneWikiⁱ	TRAM2
GenomeRNAiⁱ	9697
Protein Ontology More...PROⁱ	PR:Q15035
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000065308 Expressed in 204 organ(s), highest expression level in oocyte
CleanExⁱ	HS_TRAM2
ExpressionAtlasⁱ	Q15035 baseline and differential
Genevisibleⁱ	Q15035 HS

Family and domain databases

InterProⁱ	View protein in InterPro IPR006634 TLC-dom IPR013599 TRAM1 IPR016447 Translocation_assoc_membrane
PANTHERⁱ	PTHR12371 PTHR12371, 1 hit
Pfamⁱ	View protein in Pfam PF08390 TRAM1, 1 hit PF03798 TRAM_LAG1_CLN8, 1 hit
PIRSFⁱ	PIRSF005449 Translocation_assoc_membrane, 1 hit
SMARTⁱ	View protein in SMART SM00724 TLC, 1 hit
PROSITEⁱ	View protein in PROSITE PS50922 TLC, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	TRAM2_HUMAN
Accessionⁱ	Q15035Primary (citable) accession number: Q15035 Secondary accession number(s): A8K6T6
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	August 29, 2001
	Last sequence update:	November 1, 1996
	Last modified:	November 7, 2018
	This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
Human chromosome 6
Human chromosome 6: entries, gene names and cross-references to MIM

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UniProtKB - Q15035 (TRAM2_HUMAN)

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Translocating chain-associated membrane protein 2

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Other locations

Endoplasmic reticulum

Other locations

Topology

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Sequence cautionⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ