UniProtKB - Q15027 (ACAP1_HUMAN)
Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1
ACAP1
Functioni
GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface. Required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.
5 PublicationsMiscellaneous
Activity regulationi
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 420 – 443 | C4-typePROSITE-ProRule annotationAdd BLAST | 24 |
GO - Molecular functioni
- GTPase activator activity Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- protein transport Source: UniProtKB-KW
Keywordsi
Molecular function | GTPase activation |
Biological process | Protein transport, Transport |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q15027 |
SignaLinki | Q15027 |
SIGNORi | Q15027 |
Names & Taxonomyi
Protein namesi | Recommended name: Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1Alternative name(s): Centaurin-beta-1 Short name: Cnt-b1 |
Gene namesi | Name:ACAP1 Synonyms:CENTB1, KIAA0050 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:16467, ACAP1 |
MIMi | 607763, gene |
neXtProti | NX_Q15027 |
VEuPathDBi | HostDB:ENSG00000072818 |
Subcellular locationi
Endosome
- Recycling endosome membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication
Endosome
- recycling endosome membrane Source: UniProtKB-SubCell
Other locations
- membrane Source: UniProtKB
Keywords - Cellular componenti
Endosome, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 14 | S → A: No effect on interaction with ITGB1. 1 Publication | 1 | |
Mutagenesisi | 29 | S → A: No effect on interaction with ITGB1. 1 Publication | 1 | |
Mutagenesisi | 274 | K → N: Loss of binding to PIP2 and PIP3. Loss of association with endosomal tubules when coexpressed with PIP5K1C. 1 Publication | 1 | |
Mutagenesisi | 277 | S → A: No effect on interaction with ITGB1. 1 Publication | 1 | |
Mutagenesisi | 280 | F → A: Reduced membrane binding and ability to induce liposome tubulation. 1 Publication | 1 | |
Mutagenesisi | 280 | F → E: Almost abolishes membrane binding. 1 Publication | 1 | |
Mutagenesisi | 280 | F → W: Preserves membrane binding and ability to tubulate liposomes. 1 Publication | 1 | |
Mutagenesisi | 289 | T → A: No effect on interaction with ITGB1. 1 Publication | 1 | |
Mutagenesisi | 358 | S → A: No effect on interaction with ITGB1. 1 Publication | 1 | |
Mutagenesisi | 389 | T → A: No effect on interaction with ITGB1. 1 Publication | 1 | |
Mutagenesisi | 448 | R → Q: Loss of GAP activity. No effect on GULP1 binding or association with endosomal tubules when coexpressed with PIP5K1C. 2 Publications | 1 | |
Mutagenesisi | 461 | T → A: No effect on interaction with ITGB1. 1 Publication | 1 | |
Mutagenesisi | 554 | S → A: Loss of phosphorylation by PKB, interaction with ITGB1 and ITGB1-dependent cell migration. 2 Publications | 1 | |
Mutagenesisi | 554 | S → D: Enhances interaction with ITGB1. 2 Publications | 1 | |
Mutagenesisi | 568 | S → A: No effect on interaction with ITGB1. 1 Publication | 1 | |
Mutagenesisi | 711 | T → A: No effect on interaction with ITGB1. 1 Publication | 1 | |
Mutagenesisi | 712 | Y → F: No effect on interaction with ITGB1. 1 Publication | 1 | |
Mutagenesisi | 724 | S → A: Loss of phosphorylation at S-554, interaction with ITGB1 and ITGB1-dependent cell migration. 1 Publication | 1 | |
Mutagenesisi | 724 | S → D: Enhances interaction with ITGB1. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 9744 |
OpenTargetsi | ENSG00000072818 |
PharmGKBi | PA26406 |
Miscellaneous databases
Pharosi | Q15027, Tbio |
Genetic variation databases
BioMutai | ACAP1 |
DMDMi | 3183210 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000074209 | 1 – 740 | Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1Add BLAST | 740 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 485 | 3'-nitrotyrosineCombined sources | 1 | |
Modified residuei | 554 | Phosphoserine; by PKB1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Nitration, PhosphoproteinProteomic databases
EPDi | Q15027 |
jPOSTi | Q15027 |
MassIVEi | Q15027 |
MaxQBi | Q15027 |
PaxDbi | Q15027 |
PeptideAtlasi | Q15027 |
PRIDEi | Q15027 |
ProteomicsDBi | 60378 |
PTM databases
GlyGeni | Q15027, 2 sites, 2 O-linked glycans (2 sites) |
iPTMneti | Q15027 |
PhosphoSitePlusi | Q15027 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000072818, Expressed in testis and 221 other tissues |
ExpressionAtlasi | Q15027, baseline and differential |
Genevisiblei | Q15027, HS |
Organism-specific databases
HPAi | ENSG00000072818, Tissue enhanced (bone marrow, intestine, lymphoid tissue) |
Interactioni
Subunit structurei
Banana-shaped homodimer laterally assembling into tetramers, the tetramers further pack helically onto the membrane.
Interacts with GTP-bound ARF6.
Interacts with third cytoplasmic loop of SLC2A4/GLUT4.
Interacts with CLTC.
Interacts with GULP1.
Forms a complex with GDP-bound ARF6 and GULP1.
Interacts with ITGB1; required for ITGB1 recycling.
6 PublicationsBinary interactionsi
Q15027
Protein-protein interaction databases
BioGRIDi | 115092, 14 interactors |
CORUMi | Q15027 |
IntActi | Q15027, 17 interactors |
MINTi | Q15027 |
STRINGi | 9606.ENSP00000158762 |
Miscellaneous databases
RNActi | Q15027, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q15027 |
SMRi | Q15027 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q15027 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 226 | BARAdd BLAST | 226 | |
Domaini | 265 – 360 | PHPROSITE-ProRule annotationAdd BLAST | 96 | |
Domaini | 405 – 527 | Arf-GAPPROSITE-ProRule annotationAdd BLAST | 123 | |
Repeati | 606 – 635 | ANK 1Add BLAST | 30 | |
Repeati | 639 – 668 | ANK 2Add BLAST | 30 | |
Repeati | 672 – 702 | ANK 3Add BLAST | 31 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 382 | Required for formation of endosomal tubules when overexpressed with PIP5K1CAdd BLAST | 382 | |
Regioni | 405 – 740 | Required for interaction with GULP1Add BLAST | 336 | |
Regioni | 525 – 581 | DisorderedSequence analysisAdd BLAST | 57 | |
Regioni | 525 – 566 | Prevents interaction with ITGB1 when S-554 is not phosphorylatedAdd BLAST | 42 |
Domaini
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 420 – 443 | C4-typePROSITE-ProRule annotationAdd BLAST | 24 |
Keywords - Domaini
ANK repeat, Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG0521, Eukaryota |
GeneTreei | ENSGT00940000160289 |
HOGENOMi | CLU_012513_0_0_1 |
InParanoidi | Q15027 |
OMAi | QATYFQQ |
OrthoDBi | 751525at2759 |
PhylomeDBi | Q15027 |
TreeFami | TF318315 |
Family and domain databases
Gene3Di | 1.10.220.150, 1 hit 1.20.1270.60, 1 hit 1.25.40.20, 1 hit 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR045258, ACAP1/2/3-like IPR027267, AH/BAR_dom_sf IPR002110, Ankyrin_rpt IPR036770, Ankyrin_rpt-contain_sf IPR037278, ARFGAP/RecO IPR001164, ArfGAP_dom IPR038508, ArfGAP_dom_sf IPR011993, PH-like_dom_sf IPR001849, PH_domain |
PANTHERi | PTHR23180, PTHR23180, 1 hit |
Pfami | View protein in Pfam PF12796, Ank_2, 1 hit PF01412, ArfGap, 1 hit PF00169, PH, 1 hit |
PRINTSi | PR00405, REVINTRACTNG |
SMARTi | View protein in SMART SM00248, ANK, 3 hits SM00105, ArfGap, 1 hit SM00233, PH, 1 hit |
SUPFAMi | SSF103657, SSF103657, 1 hit SSF48403, SSF48403, 1 hit SSF57863, SSF57863, 1 hit |
PROSITEi | View protein in PROSITE PS50297, ANK_REP_REGION, 1 hit PS50088, ANK_REPEAT, 2 hits PS50115, ARFGAP, 1 hit PS50003, PH_DOMAIN, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MTVKLDFEEC LKDSPRFRAS IELVEAEVSE LETRLEKLLK LGTGLLESGR
60 70 80 90 100
HYLAASRAFV VGICDLARLG PPEPMMAECL EKFTVSLNHK LDSHAELLDA
110 120 130 140 150
TQHTLQQQIQ TLVKEGLRGF REARRDFWRG AESLEAALTH NAEVPRRRAQ
160 170 180 190 200
EAEEAGAALR TARAGYRGRA LDYALQINVI EDKRKFDIME FVLRLVEAQA
210 220 230 240 250
THFQQGHEEL SRLSQYRKEL GAQLHQLVLN SAREKRDMEQ RHVLLKQKEL
260 270 280 290 300
GGEEPEPSLR EGPGGLVMEG HLFKRASNAF KTWSRRWFTI QSNQLVYQKK
310 320 330 340 350
YKDPVTVVVD DLRLCTVKLC PDSERRFCFE VVSTSKSCLL QADSERLLQL
360 370 380 390 400
WVSAVQSSIA SAFSQARLDD SPRGPGQGSG HLAIGSAATL GSGGMARGRE
410 420 430 440 450
PGGVGHVVAQ VQSVDGNAQC CDCREPAPEW ASINLGVTLC IQCSGIHRSL
460 470 480 490 500
GVHFSKVRSL TLDSWEPELV KLMCELGNVI INQIYEARVE AMAVKKPGPS
510 520 530 540 550
CSRQEKEAWI HAKYVEKKFL TKLPEIRGRR GGRGRPRGQP PVPPKPSIRP
560 570 580 590 600
RPGSLRSKPE PPSEDLGSLH PGALLFRASG HPPSLPTMAD ALAHGADVNW
610 620 630 640 650
VNGGQDNATP LIQATAANSL LACEFLLQNG ANVNQADSAG RGPLHHATIL
660 670 680 690 700
GHTGLACLFL KRGADLGARD SEGRDPLTIA METANADIVT LLRLAKMREA
710 720 730 740
EAAQGQAGDE TYLDIFRDFS LMASDDPEKL SRRSHDLHTL
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketI3L0K9 | I3L0K9_HUMAN | Arf-GAP with coiled-coil, ANK repea... | ACAP1 | 148 | Annotation score: | ||
I3L268 | I3L268_HUMAN | Arf-GAP with coiled-coil, ANK repea... | ACAP1 | 101 | Annotation score: |
Sequence cautioni
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_048328 | 68 | R → C. Corresponds to variant dbSNP:rs35933585Ensembl. | 1 | |
Natural variantiVAR_036178 | 114 | K → R in a breast cancer sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs759855054Ensembl. | 1 | |
Natural variantiVAR_036179 | 129 | R → Q in a colorectal cancer sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs754740225Ensembl. | 1 | |
Natural variantiVAR_048329 | 533 | R → W. Corresponds to variant dbSNP:rs35019942Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D30758 mRNA Translation: BAA06418.2 Different initiation. BT009788 mRNA Translation: AAP88790.1 BC018543 mRNA Translation: AAH18543.1 |
CCDSi | CCDS11101.1 |
RefSeqi | NP_055531.1, NM_014716.3 |
Genome annotation databases
Ensembli | ENST00000158762.8; ENSP00000158762.3; ENSG00000072818.12 ENST00000672212.1; ENSP00000499859.1; ENSG00000288169.1 |
GeneIDi | 9744 |
KEGGi | hsa:9744 |
MANE-Selecti | ENST00000158762.8; ENSP00000158762.3; NM_014716.4; NP_055531.1 |
UCSCi | uc002ggd.3, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D30758 mRNA Translation: BAA06418.2 Different initiation. BT009788 mRNA Translation: AAP88790.1 BC018543 mRNA Translation: AAH18543.1 |
CCDSi | CCDS11101.1 |
RefSeqi | NP_055531.1, NM_014716.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3JUE | X-ray | 2.30 | A/B | 378-740 | [»] | |
3T9K | X-ray | 2.30 | A/B | 378-740 | [»] | |
4CKG | electron microscopy | 12.00 | A/B/C/D | 1-377 | [»] | |
4CKH | electron microscopy | 14.00 | A/B/C/D | 1-377 | [»] | |
4F1P | X-ray | 2.30 | A/B | 378-740 | [»] | |
4NSW | X-ray | 2.20 | A/B | 1-377 | [»] | |
5H3D | electron microscopy | 14.00 | A/B/C/D | 1-377 | [»] | |
AlphaFoldDBi | Q15027 | |||||
SMRi | Q15027 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 115092, 14 interactors |
CORUMi | Q15027 |
IntActi | Q15027, 17 interactors |
MINTi | Q15027 |
STRINGi | 9606.ENSP00000158762 |
PTM databases
GlyGeni | Q15027, 2 sites, 2 O-linked glycans (2 sites) |
iPTMneti | Q15027 |
PhosphoSitePlusi | Q15027 |
Genetic variation databases
BioMutai | ACAP1 |
DMDMi | 3183210 |
Proteomic databases
EPDi | Q15027 |
jPOSTi | Q15027 |
MassIVEi | Q15027 |
MaxQBi | Q15027 |
PaxDbi | Q15027 |
PeptideAtlasi | Q15027 |
PRIDEi | Q15027 |
ProteomicsDBi | 60378 |
Protocols and materials databases
Antibodypediai | 24069, 246 antibodies from 34 providers |
DNASUi | 9744 |
Genome annotation databases
Ensembli | ENST00000158762.8; ENSP00000158762.3; ENSG00000072818.12 ENST00000672212.1; ENSP00000499859.1; ENSG00000288169.1 |
GeneIDi | 9744 |
KEGGi | hsa:9744 |
MANE-Selecti | ENST00000158762.8; ENSP00000158762.3; NM_014716.4; NP_055531.1 |
UCSCi | uc002ggd.3, human |
Organism-specific databases
CTDi | 9744 |
DisGeNETi | 9744 |
GeneCardsi | ACAP1 |
HGNCi | HGNC:16467, ACAP1 |
HPAi | ENSG00000072818, Tissue enhanced (bone marrow, intestine, lymphoid tissue) |
MIMi | 607763, gene |
neXtProti | NX_Q15027 |
OpenTargetsi | ENSG00000072818 |
PharmGKBi | PA26406 |
VEuPathDBi | HostDB:ENSG00000072818 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0521, Eukaryota |
GeneTreei | ENSGT00940000160289 |
HOGENOMi | CLU_012513_0_0_1 |
InParanoidi | Q15027 |
OMAi | QATYFQQ |
OrthoDBi | 751525at2759 |
PhylomeDBi | Q15027 |
TreeFami | TF318315 |
Enzyme and pathway databases
PathwayCommonsi | Q15027 |
SignaLinki | Q15027 |
SIGNORi | Q15027 |
Miscellaneous databases
BioGRID-ORCSi | 9744, 9 hits in 1075 CRISPR screens |
ChiTaRSi | ACAP1, human |
EvolutionaryTracei | Q15027 |
GeneWikii | CENTB1 |
GenomeRNAii | 9744 |
Pharosi | Q15027, Tbio |
PROi | PR:Q15027 |
RNActi | Q15027, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000072818, Expressed in testis and 221 other tissues |
ExpressionAtlasi | Q15027, baseline and differential |
Genevisiblei | Q15027, HS |
Family and domain databases
Gene3Di | 1.10.220.150, 1 hit 1.20.1270.60, 1 hit 1.25.40.20, 1 hit 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR045258, ACAP1/2/3-like IPR027267, AH/BAR_dom_sf IPR002110, Ankyrin_rpt IPR036770, Ankyrin_rpt-contain_sf IPR037278, ARFGAP/RecO IPR001164, ArfGAP_dom IPR038508, ArfGAP_dom_sf IPR011993, PH-like_dom_sf IPR001849, PH_domain |
PANTHERi | PTHR23180, PTHR23180, 1 hit |
Pfami | View protein in Pfam PF12796, Ank_2, 1 hit PF01412, ArfGap, 1 hit PF00169, PH, 1 hit |
PRINTSi | PR00405, REVINTRACTNG |
SMARTi | View protein in SMART SM00248, ANK, 3 hits SM00105, ArfGap, 1 hit SM00233, PH, 1 hit |
SUPFAMi | SSF103657, SSF103657, 1 hit SSF48403, SSF48403, 1 hit SSF57863, SSF57863, 1 hit |
PROSITEi | View protein in PROSITE PS50297, ANK_REP_REGION, 1 hit PS50088, ANK_REPEAT, 2 hits PS50115, ARFGAP, 1 hit PS50003, PH_DOMAIN, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ACAP1_HUMAN | |
Accessioni | Q15027Primary (citable) accession number: Q15027 Secondary accession number(s): Q53XN9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | November 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 197 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 17
Human chromosome 17: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references