UniProtKB - Q15025 (TNIP1_HUMAN)
TNFAIP3-interacting protein 1
TNIP1
Functioni
Inhibits NF-kappa-B activation and TNF-induced NF-kappa-B-dependent gene expression by regulating A20/TNFAIP3-mediated deubiquitination of IKBKG; proposed to link A20/TNFAIP3 to ubiquitinated IKBKG. Involved in regulation of EGF-induced ERK1/ERK2 signaling pathway; blocks MAPK3/MAPK1 nuclear translocation and MAPK1-dependent transcription. Increases cell surface CD4(T4) antigen expression. Involved in the anti-inflammatory response of macrophages and positively regulates TLR-induced activation of CEBPB. Involved in the prevention of autoimmunity; this function implicates binding to polyubiquitin. Involved in leukocyte integrin activation during inflammation; this function is mediated by association with SELPLG and dependent on phosphorylation by SRC-family kinases. Interacts with HIV-1 matrix protein and is packaged into virions and overexpression can inhibit viral replication. May regulate matrix nuclear localization, both nuclear import of PIC (Preintegration complex) and export of GAG polyprotein and viral genomic RNA during virion production. In case of infection, promotes association of IKBKG with Shigella flexneri E3 ubiquitin-protein ligase ipah9.8 p which in turn promotes polyubiquitination of IKBKG leading to its proteasome-dependent degradation and thus is perturbing NF-kappa-B activation during bacterial infection.
5 PublicationsGO - Molecular functioni
- identical protein binding Source: IntAct
- mitogen-activated protein kinase binding Source: UniProtKB
GO - Biological processi
- cellular response to lipopolysaccharide Source: GO_Central
- defense response Source: ProtInc
- glycoprotein biosynthetic process Source: UniProtKB
- inflammatory response Source: UniProtKB-KW
- leukocyte cell-cell adhesion Source: UniProtKB
- MyD88-dependent toll-like receptor signaling pathway Source: UniProtKB
- negative regulation of ERK1 and ERK2 cascade Source: UniProtKB
- negative regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
- negative regulation of viral genome replication Source: UniProtKB
- positive regulation of inflammatory response Source: UniProtKB
- positive regulation of protein deubiquitination Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: UniProtKB
- regulation of transcription by RNA polymerase II Source: GO_Central
- translation Source: ProtInc
Keywordsi
Biological process | Inflammatory response |
Enzyme and pathway databases
PathwayCommonsi | Q15025 |
Reactomei | R-HSA-5689896, Ovarian tumor domain proteases |
SignaLinki | Q15025 |
Names & Taxonomyi
Protein namesi | Recommended name: TNFAIP3-interacting protein 1Alternative name(s): A20-binding inhibitor of NF-kappa-B activation 1 Short name: ABIN-1 HIV-1 Nef-interacting protein Nef-associated factor 1 Short name: Naf1 Nip40-1 Virion-associated nuclear shuttling protein Short name: VAN Short name: hVAN |
Gene namesi | Name:TNIP1 Synonyms:KIAA0113, NAF1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:16903, TNIP1 |
MIMi | 607714, gene |
neXtProti | NX_Q15025 |
VEuPathDBi | HostDB:ENSG00000145901 |
Subcellular locationi
Cytosol
- cytosol Source: HPA
Nucleus
- nucleoplasm Source: HPA
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 472 | D → N: Abolishes binding to polyubiquitin ('K-63'-linked and linear). 1 Publication | 1 | |
Mutagenesisi | 552 | Y → F: Abolishes interaction with PI3K p85 regulatory subunit and abolishes interaction between SELPLG and PI3K p85 regulatory subunit. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 10318 |
MalaCardsi | TNIP1 |
OpenTargetsi | ENSG00000145901 |
Orphaneti | 536, Systemic lupus erythematosus |
PharmGKBi | PA128394573 |
Miscellaneous databases
Pharosi | Q15025, Tbio |
Genetic variation databases
BioMutai | TNIP1 |
DMDMi | 20138952 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000096691 | 1 – 636 | TNFAIP3-interacting protein 1Add BLAST | 636 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 77 | PhosphoserineCombined sources | 1 | |
Modified residuei | 284 | PhosphoserineCombined sources | 1 | |
Modified residuei | 403 | PhosphoserineCombined sources | 1 | |
Modified residuei | 438 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 442 | PhosphoserineCombined sources | 1 | |
Modified residuei | 552 | Phosphotyrosine1 Publication | 1 | |
Modified residuei | 571 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 599 | Asymmetric dimethylarginine; alternateCombined sources | 1 | |
Modified residuei | 599 | Omega-N-methylarginine; alternateCombined sources | 1 | |
Modified residuei | 627 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Methylation, PhosphoproteinProteomic databases
EPDi | Q15025 |
jPOSTi | Q15025 |
MassIVEi | Q15025 |
MaxQBi | Q15025 |
PaxDbi | Q15025 |
PeptideAtlasi | Q15025 |
PRIDEi | Q15025 |
ProteomicsDBi | 17465 18155 60376 [Q15025-1] 60377 [Q15025-2] |
PTM databases
iPTMneti | Q15025 |
PhosphoSitePlusi | Q15025 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000145901, Expressed in amniotic fluid and 247 other tissues |
ExpressionAtlasi | Q15025, baseline and differential |
Genevisiblei | Q15025, HS |
Organism-specific databases
HPAi | ENSG00000145901, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with TNFAIP3 and IKBKG (polyubiquitinated); facilitates TNFAIP3-mediated de-ubiquitination of NEMO/IKBKG.
Interacts with polyubiquitin.
Interacts with MAPK1, SELPLG and PIK3CD.
Interacts with IRAK1 (polyubiquitinated).
Interacts with MYD88; the interaction is indicative for participation in an activated TLR-signaling complex.
Interacts with HIV-1 matrix protein.
Interacts with Shigella flexneri ipah9.8; the interaction promotes polyubiquitination of IKBKG.
4 PublicationsBinary interactionsi
Q15025
GO - Molecular functioni
- identical protein binding Source: IntAct
- mitogen-activated protein kinase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 115602, 209 interactors |
CORUMi | Q15025 |
DIPi | DIP-27577N |
IntActi | Q15025, 165 interactors |
MINTi | Q15025 |
STRINGi | 9606.ENSP00000317891 |
Miscellaneous databases
RNActi | Q15025, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q15025 |
SMRi | Q15025 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 61 – 151 | DisorderedSequence analysisAdd BLAST | 91 | |
Regioni | 94 – 412 | Interaction with NefAdd BLAST | 319 | |
Regioni | 252 – 283 | DisorderedSequence analysisAdd BLAST | 32 | |
Regioni | 351 – 367 | Interaction with Shigella flexneri ipah9.8Add BLAST | 17 | |
Regioni | 431 – 588 | Required for inhibitory activity of TNF-induced NF-kappa-B activationBy similarityAdd BLAST | 158 | |
Regioni | 452 – 510 | Ubiquitin-binding domain (UBD)Add BLAST | 59 | |
Regioni | 603 – 636 | DisorderedSequence analysisAdd BLAST | 34 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 20 – 73 | Sequence analysisAdd BLAST | 54 | |
Coiled coili | 196 – 258 | Sequence analysisAdd BLAST | 63 | |
Coiled coili | 294 – 535 | Sequence analysisAdd BLAST | 242 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 524 – 530 | Nuclear localization signalSequence analysis | 7 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 114 – 142 | Polar residuesSequence analysisAdd BLAST | 29 | |
Compositional biasi | 604 – 618 | Polar residuesSequence analysisAdd BLAST | 15 |
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | ENOG502QPYT, Eukaryota |
GeneTreei | ENSGT00510000046908 |
HOGENOMi | CLU_033970_0_0_1 |
InParanoidi | Q15025 |
OMAi | HPMYHGY |
OrthoDBi | 701614at2759 |
PhylomeDBi | Q15025 |
TreeFami | TF351138 |
Family and domain databases
InterProi | View protein in InterPro IPR033372, TNIP1 |
PANTHERi | PTHR31882:SF3, PTHR31882:SF3, 1 hit |
s (8+)i Sequence
Sequence statusi: Complete.
This entry describes 8 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 8 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEGRGPYRIY DPGGSVPSGE ASAAFERLVK ENSRLKEKMQ GIKMLGELLE
60 70 80 90 100
ESQMEATRLR QKAEELVKDN ELLPPPSPSL GSFDPLAELT GKDSNVTASP
110 120 130 140 150
TAPACPSDKP APVQKPPSSG TSSEFEVVTP EEQNSPESSS HANAMALGPL
160 170 180 190 200
PREDGNLMLH LQRLETTLSV CAEEPDHGQL FTHLGRMALE FNRLASKVHK
210 220 230 240 250
NEQRTSILQT LCEQLRKENE ALKAKLDKGL EQRDQAAERL REENLELKKL
260 270 280 290 300
LMSNGNKEGA SGRPGSPKME GTGKKAVAGQ QQASVTAGKV PEVVALGAAE
310 320 330 340 350
KKVKMLEQQR SELLEVNKQW DQHFRSMKQQ YEQKITELRQ KLADLQKQVT
360 370 380 390 400
DLEAEREQKQ RDFDRKLLLA KSKIEMEETD KEQLTAEAKE LRQKVKYLQD
410 420 430 440 450
QLSPLTRQRE YQEKEIQRLN KALEEALSIQ TPPSSPPTAF GSPEGAGALL
460 470 480 490 500
RKQELVTQNE LLKQQVKIFE EDFQRERSDR ERMNEEKEEL KKQVEKLQAQ
510 520 530 540 550
VTLSNAQLKA FKDEEKAREA LRQQKRKAKA SGERYHVEPH PEHLCGAYPY
560 570 580 590 600
AYPPMPAMVP HHGFEDWSQI RYPPPPMAME HPPPLPNSRL FHLPEYTWRL
610 620 630
PCGGVRNPNQ SSQVMDPPTA RPTEPESPKN DREGPQ
The sequence of this isoform differs from the canonical sequence as follows:
530-556: ASGERYHVEPHPEHLCGAYPYAYPPMP → SLQKMTVRGLSETRLCHLAPPSSCRAS
557-636: Missing.
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0A0MRZ4 | A0A0A0MRZ4_HUMAN | TNFAIP3-interacting protein 1 | TNIP1 | 619 | Annotation score: | ||
E7EW15 | E7EW15_HUMAN | TNFAIP3-interacting protein 1 | TNIP1 | 257 | Annotation score: | ||
E7EMV7 | E7EMV7_HUMAN | TNFAIP3-interacting protein 1 | TNIP1 | 492 | Annotation score: | ||
H0YC17 | H0YC17_HUMAN | TNFAIP3-interacting protein 1 | TNIP1 | 146 | Annotation score: | ||
E7EW68 | E7EW68_HUMAN | TNFAIP3-interacting protein 1 | TNIP1 | 143 | Annotation score: | ||
E7EWG2 | E7EWG2_HUMAN | TNFAIP3-interacting protein 1 | TNIP1 | 133 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 148 | G → D in AAH12133 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 178 | G → S in BAH13185 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 299 | A → P in AAG42154 (PubMed:11090181).Curated | 1 | |
Sequence conflicti | 299 | A → P in BAF34946 (PubMed:17016622).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_051453 | 103 | P → S. Corresponds to variant dbSNP:rs2303018Ensembl. | 1 | |
Natural variantiVAR_051454 | 146 | A → V. Corresponds to variant dbSNP:rs2233289EnsemblClinVar. | 1 | |
Natural variantiVAR_051455 | 151 | P → A. Corresponds to variant dbSNP:rs2233290Ensembl. | 1 | |
Natural variantiVAR_051456 | 233 | R → Q. Corresponds to variant dbSNP:rs2233292Ensembl. | 1 | |
Natural variantiVAR_051457 | 260 | A → V. Corresponds to variant dbSNP:rs2233295EnsemblClinVar. | 1 | |
Natural variantiVAR_067965 | 263 | R → W in patients with gastrointestinal diffuse large cell lymphoma. 1 PublicationCorresponds to variant dbSNP:rs117663772Ensembl. | 1 | |
Natural variantiVAR_067966 | 286 | T → M in patients with gastrointestinal diffuse large cell lymphoma; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs185683917Ensembl. | 1 | |
Natural variantiVAR_067967 | 374 | I → T in patients with gastrointestinal diffuse large cell lymphoma. 1 PublicationCorresponds to variant dbSNP:rs748495842Ensembl. | 1 | |
Natural variantiVAR_067968 | 476 | E → K in patients with gastrointestinal diffuse large cell lymphoma; somatic mutation; loss of inhibitory activity on CARD11- and TNF-induced NF-kappa-B activation. 1 Publication | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_045296 | 1 – 53 | Missing in isoform 3. 3 PublicationsAdd BLAST | 53 | |
Alternative sequenceiVSP_055208 | 530 – 593 | Missing in isoform 4 and isoform 6. 1 PublicationAdd BLAST | 64 | |
Alternative sequenceiVSP_055209 | 530 – 556 | ASGER…YPPMP → SLQKMTVRGLSETRLCHLAP PSSCRAS in isoform 5. 1 PublicationAdd BLAST | 27 | |
Alternative sequenceiVSP_055210 | 530 – 552 | ASGER…YPYAY → SQLISDCQETRSHLHGVARA SAG in isoform 8. 1 PublicationAdd BLAST | 23 | |
Alternative sequenceiVSP_055211 | 530 – 545 | ASGER…PEHLC → GTHRGCPRRLPERKVK in isoform 7. 1 PublicationAdd BLAST | 16 | |
Alternative sequenceiVSP_055212 | 546 – 636 | Missing in isoform 7. 1 PublicationAdd BLAST | 91 | |
Alternative sequenceiVSP_055213 | 553 – 636 | Missing in isoform 8. 1 PublicationAdd BLAST | 84 | |
Alternative sequenceiVSP_055214 | 557 – 636 | Missing in isoform 5. 1 PublicationAdd BLAST | 80 | |
Alternative sequenceiVSP_003913 | 627 – 636 | SPKNDREGPQ → PADLRLPRN in isoform 2 and isoform 6. 3 Publications | 10 |
Sequence databases
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
7EAL | X-ray | 2.50 | B/C/E/F | 405-513 | [»] | |
7EAO | X-ray | 2.90 | B/C | 415-513 | [»] | |
7EB9 | X-ray | 3.20 | B/C | 415-513 | [»] | |
AlphaFoldDBi | Q15025 | |||||
SMRi | Q15025 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 115602, 209 interactors |
CORUMi | Q15025 |
DIPi | DIP-27577N |
IntActi | Q15025, 165 interactors |
MINTi | Q15025 |
STRINGi | 9606.ENSP00000317891 |
PTM databases
iPTMneti | Q15025 |
PhosphoSitePlusi | Q15025 |
Genetic variation databases
BioMutai | TNIP1 |
DMDMi | 20138952 |
Proteomic databases
EPDi | Q15025 |
jPOSTi | Q15025 |
MassIVEi | Q15025 |
MaxQBi | Q15025 |
PaxDbi | Q15025 |
PeptideAtlasi | Q15025 |
PRIDEi | Q15025 |
ProteomicsDBi | 17465 18155 60376 [Q15025-1] 60377 [Q15025-2] |
Protocols and materials databases
Antibodypediai | 28121, 304 antibodies from 28 providers |
DNASUi | 10318 |
Genome annotation databases
Organism-specific databases
CTDi | 10318 |
DisGeNETi | 10318 |
GeneCardsi | TNIP1 |
HGNCi | HGNC:16903, TNIP1 |
HPAi | ENSG00000145901, Low tissue specificity |
MalaCardsi | TNIP1 |
MIMi | 607714, gene |
neXtProti | NX_Q15025 |
OpenTargetsi | ENSG00000145901 |
Orphaneti | 536, Systemic lupus erythematosus |
PharmGKBi | PA128394573 |
VEuPathDBi | HostDB:ENSG00000145901 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QPYT, Eukaryota |
GeneTreei | ENSGT00510000046908 |
HOGENOMi | CLU_033970_0_0_1 |
InParanoidi | Q15025 |
OMAi | HPMYHGY |
OrthoDBi | 701614at2759 |
PhylomeDBi | Q15025 |
TreeFami | TF351138 |
Enzyme and pathway databases
PathwayCommonsi | Q15025 |
Reactomei | R-HSA-5689896, Ovarian tumor domain proteases |
SignaLinki | Q15025 |
Miscellaneous databases
BioGRID-ORCSi | 10318, 17 hits in 1078 CRISPR screens |
ChiTaRSi | TNIP1, human |
GeneWikii | TNIP1 |
GenomeRNAii | 10318 |
Pharosi | Q15025, Tbio |
PROi | PR:Q15025 |
RNActi | Q15025, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000145901, Expressed in amniotic fluid and 247 other tissues |
ExpressionAtlasi | Q15025, baseline and differential |
Genevisiblei | Q15025, HS |
Family and domain databases
InterProi | View protein in InterPro IPR033372, TNIP1 |
PANTHERi | PTHR31882:SF3, PTHR31882:SF3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TNIP1_HUMAN | |
Accessioni | Q15025Primary (citable) accession number: Q15025 Secondary accession number(s): A4F1W8 Q9H1J3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 23, 2002 |
Last sequence update: | August 1, 1999 | |
Last modified: | May 25, 2022 | |
This is version 195 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 5
Human chromosome 5: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references