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Protein

Squamous cell carcinoma antigen recognized by T-cells 3

Gene

SART3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

U6 snRNP-binding protein that functions as a recycling factor of the splicing machinery. Promotes the initial reassembly of U4 and U6 snRNPs following their ejection from the spliceosome during its maturation (PubMed:12032085). Also binds U6atac snRNPs and may function as a recycling factor for U4atac/U6atac spliceosomal snRNP, an initial step in the assembly of U12-type spliceosomal complex. The U12-type spliceosomal complex plays a role in the splicing of introns with non-canonical splice sites (PubMed:14749385). May also function as a substrate-targeting factor for deubiquitinases like USP4 and USP15. Recruits USP4 to ubiquitinated PRPF3 within the U4/U5/U6 tri-snRNP complex, promoting PRPF3 deubiquitination and thereby regulating the spliceosome U4/U5/U6 tri-snRNP spliceosomal complex disassembly (PubMed:20595234). May also recruit the deubiquitinase USP15 to histone H2B and mediate histone deubiquitination, thereby regulating gene expression and/or DNA repair (PubMed:24526689). May play a role in hematopoiesis probably through transcription regulation of specific genes including MYC (By similarity).By similarity4 Publications
Regulates Tat transactivation activity through direct interaction. May be a cellular factor for HIV-1 gene expression and viral replication.1 Publication

GO - Molecular functioni

  • histone binding Source: UniProtKB
  • RNA binding Source: UniProtKB
  • U4 snRNA binding Source: UniProtKB
  • U6atac snRNA binding Source: UniProtKB
  • U6 snRNA binding Source: UniProtKB
  • ubiquitin-specific protease binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processmRNA processing, mRNA splicing

Names & Taxonomyi

Protein namesi
Recommended name:
Squamous cell carcinoma antigen recognized by T-cells 3Curated
Short name:
SART-3Imported
Alternative name(s):
Tat-interacting protein of 110 kDa1 Publication
Short name:
Tip1101 Publication
p110 nuclear RNA-binding protein1 Publication
Gene namesi
Name:SART3Imported
Synonyms:KIAA0156Imported, TIP1101 Publication
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000075856.11
HGNCiHGNC:16860 SART3
MIMi611684 gene
neXtProtiNX_Q15020

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi9733
OpenTargetsiENSG00000075856
PharmGKBiPA34948

Polymorphism and mutation databases

BioMutaiSART3
DMDMi74762140

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00002233132 – 963Squamous cell carcinoma antigen recognized by T-cells 3Add BLAST962

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1 Publication1
Modified residuei10PhosphoserineCombined sources1
Modified residuei16PhosphoserineCombined sources1
Modified residuei215PhosphoserineCombined sources1
Modified residuei650PhosphoserineCombined sources1
Modified residuei657PhosphothreonineCombined sources1
Modified residuei769PhosphoserineCombined sources1
Modified residuei795PhosphoserineCombined sources1
Modified residuei852PhosphoserineCombined sources1
Modified residuei906Omega-N-methylarginineCombined sources1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiQ15020
MaxQBiQ15020
PaxDbiQ15020
PeptideAtlasiQ15020
PRIDEiQ15020
ProteomicsDBi60370
60371 [Q15020-2]
60372 [Q15020-3]

PTM databases

iPTMnetiQ15020
PhosphoSitePlusiQ15020

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiENSG00000075856 Expressed in 232 organ(s), highest expression level in endothelial cell
CleanExiHS_SART3
ExpressionAtlasiQ15020 baseline and differential
GenevisibleiQ15020 HS

Organism-specific databases

HPAiHPA044322

Interactioni

Subunit structurei

Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs (PubMed:17643375). Interacts with AGO1 and AGO2 (PubMed:17932509). Interacts with PRPF3 and USP4; the interaction with PRPF3 is direct and recruits USP4 to its substrate PRPF3 (PubMed:15314151, PubMed:20595234). Interacts with USP15; the interaction is direct (PubMed:24526689). Interacts with HIV-1 Tat (PubMed:11959860).6 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi115082, 194 interactors
IntActiQ15020, 48 interactors
MINTiQ15020
STRINGi9606.ENSP00000228284

Structurei

Secondary structure

1963
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ15020
SMRiQ15020
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ15020

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati126 – 158HAT 1Add BLAST33
Repeati164 – 195HAT 2Add BLAST32
Repeati201 – 237HAT 3Add BLAST37
Repeati242 – 275HAT 4Add BLAST34
Repeati324 – 356HAT 5Add BLAST33
Repeati359 – 391HAT 6Add BLAST33
Repeati394 – 430HAT 7Add BLAST37
Repeati487 – 520HAT 8Add BLAST34
Domaini704 – 782RRM 1PROSITE-ProRule annotationAdd BLAST79
Domaini801 – 878RRM 2PROSITE-ProRule annotationAdd BLAST78

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 351Mediates interaction with PRPF31 PublicationAdd BLAST350
Regioni487 – 520Required for interaction with USP41 PublicationAdd BLAST34
Regioni537 – 953Necessary and sufficient for U6 snRNA binding1 PublicationAdd BLAST417
Regioni600 – 670Required for nuclear localization1 PublicationAdd BLAST71

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili21 – 46Sequence analysisAdd BLAST26
Coiled coili82 – 110Sequence analysisAdd BLAST29
Coiled coili559 – 619Sequence analysisAdd BLAST61

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi601 – 617Nuclear localization signalSequence analysisAdd BLAST17

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi89 – 92Poly-Glu4
Compositional biasi612 – 616Poly-Lys5

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG0128 Eukaryota
ENOG410XP88 LUCA
GeneTreeiENSGT00900000141107
HOGENOMiHOG000063708
HOVERGENiHBG053888
InParanoidiQ15020
KOiK22611
OMAiSQAVMKM
OrthoDBiEOG091G041L
PhylomeDBiQ15020
TreeFamiTF317554

Family and domain databases

CDDicd12391 RRM1_SART3, 1 hit
cd12392 RRM2_SART3, 1 hit
Gene3Di1.25.40.10, 2 hits
3.30.70.330, 2 hits
InterProiView protein in InterPro
IPR003107 HAT
IPR008669 LSM_interact
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
IPR034217 SART3_RRM1
IPR034218 SART3_RRM2
IPR011990 TPR-like_helical_dom_sf
PfamiView protein in Pfam
PF05391 Lsm_interact, 1 hit
PF00076 RRM_1, 2 hits
SMARTiView protein in SMART
SM00386 HAT, 7 hits
SM00360 RRM, 2 hits
SUPFAMiSSF48452 SSF48452, 1 hit
SSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 2 hits

Sequences (4+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 5 potential isoforms that are computationally mapped.iShow all

Isoform 1 (identifier: Q15020-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MATAAETSAS EPEAESKAGP KADGEEDEVK AARTRRKVLS RAVAAATYKT
60 70 80 90 100
MGPAWDQQEE GVSESDGDEY AMASSAESSP GEYEWEYDEE EEKNQLEIER
110 120 130 140 150
LEEQLSINVY DYNCHVDLIR LLRLEGELTK VRMARQKMSE IFPLTEELWL
160 170 180 190 200
EWLHDEISMA QDGLDREHVY DLFEKAVKDY ICPNIWLEYG QYSVGGIGQK
210 220 230 240 250
GGLEKVRSVF ERALSSVGLH MTKGLALWEA YREFESAIVE AARLEKVHSL
260 270 280 290 300
FRRQLAIPLY DMEATFAEYE EWSEDPIPES VIQNYNKALQ QLEKYKPYEE
310 320 330 340 350
ALLQAEAPRL AEYQAYIDFE MKIGDPARIQ LIFERALVEN CLVPDLWIRY
360 370 380 390 400
SQYLDRQLKV KDLVLSVHNR AIRNCPWTVA LWSRYLLAME RHGVDHQVIS
410 420 430 440 450
VTFEKALNAG FIQATDYVEI WQAYLDYLRR RVDFKQDSSK ELEELRAAFT
460 470 480 490 500
RALEYLKQEV EERFNESGDP SCVIMQNWAR IEARLCNNMQ KARELWDSIM
510 520 530 540 550
TRGNAKYANM WLEYYNLERA HGDTQHCRKA LHRAVQCTSD YPEHVCEVLL
560 570 580 590 600
TMERTEGSLE DWDIAVQKTE TRLARVNEQR MKAAEKEAAL VQQEEEKAEQ
610 620 630 640 650
RKRARAEKKA LKKKKKIRGP EKRGADEDDE KEWGDDEEEQ PSKRRRVENS
660 670 680 690 700
IPAAGETQNV EVAAGPAGKC AAVDVEPPSK QKEKAASLKR DMPKVLHDSS
710 720 730 740 750
KDSITVFVSN LPYSMQEPDT KLRPLFEACG EVVQIRPIFS NRGDFRGYCY
760 770 780 790 800
VEFKEEKSAL QALEMDRKSV EGRPMFVSPC VDKSKNPDFK VFRYSTSLEK
810 820 830 840 850
HKLFISGLPF SCTKEELEEI CKAHGTVKDL RLVTNRAGKP KGLAYVEYEN
860 870 880 890 900
ESQASQAVMK MDGMTIKENI IKVAISNPPQ RKVPEKPETR KAPGGPMLLP
910 920 930 940 950
QTYGARGKGR TQLSLLPRAL QRPSAAAPQA ENGPAAAPAV AAPAATEAPK
960
MSNADFAKLF LRK
Length:963
Mass (Da):109,935
Last modified:November 1, 1996 - v1
Checksum:i06B26CEB8B19102A
GO
Isoform 2 (identifier: Q15020-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     351-364: SQYLDRQLKVKDLV → RSTTESKGFGFICT
     365-963: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. Inactive in U4/U6 snRNP recycling.1 Publication
Show »
Length:364
Mass (Da):41,829
Checksum:iD86B2562A7FA2E4E
GO
Isoform 3 (identifier: Q15020-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     105-129: LSINVYDYNCHVDLIRLLRLEGELT → VGPGVGSGHLPVFQVLGSPCPGPPP
     130-963: Missing.

Note: No experimental confirmation available.
Show »
Length:129
Mass (Da):13,773
Checksum:iB41FC49C1C5E4C33
GO
Isoform 4 (identifier: Q15020-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     401-436: Missing.

Note: No experimental confirmation available.
Show »
Length:927
Mass (Da):105,584
Checksum:iA4D08EF2F14CFC93
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F8VV04F8VV04_HUMAN
Squamous cell carcinoma antigen rec...
SART3
771Annotation score:
H0YHU8H0YHU8_HUMAN
Squamous cell carcinoma antigen rec...
SART3
144Annotation score:
F8W667F8W667_HUMAN
Squamous cell carcinoma antigen rec...
SART3
305Annotation score:
F8VVK9F8VVK9_HUMAN
Squamous cell carcinoma antigen rec...
SART3
111Annotation score:
F8VZM2F8VZM2_HUMAN
Squamous cell carcinoma antigen rec...
SART3
156Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03880223D → E1 PublicationCorresponds to variant dbSNP:rs2072579Ensembl.1
Natural variantiVAR_038683591V → M Found in a patient with disseminated superficial actinic porokeratosis; unknown pathological significance. 1 PublicationCorresponds to variant dbSNP:rs118203954EnsemblClinVar.1
Natural variantiVAR_038684621E → D. Corresponds to variant dbSNP:rs2287546Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_017248105 – 129LSINV…EGELT → VGPGVGSGHLPVFQVLGSPC PGPPP in isoform 3. 1 PublicationAdd BLAST25
Alternative sequenceiVSP_017249130 – 963Missing in isoform 3. 1 PublicationAdd BLAST834
Alternative sequenceiVSP_017250351 – 364SQYLD…VKDLV → RSTTESKGFGFICT in isoform 2. 1 PublicationAdd BLAST14
Alternative sequenceiVSP_017251365 – 963Missing in isoform 2. 1 PublicationAdd BLAST599
Alternative sequenceiVSP_057284401 – 436Missing in isoform 4. 1 PublicationAdd BLAST36

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB020880 mRNA Translation: BAA78384.1
AF387506 mRNA Translation: AAK69347.1
D63879 mRNA Translation: BAA09929.1
AK290209 mRNA Translation: BAF82898.1
AC008119 Genomic DNA No translation available.
AC010206 Genomic DNA No translation available.
KF455716 Genomic DNA No translation available.
KF511170 Genomic DNA No translation available.
BC032601 mRNA Translation: AAH32601.1
BC041638 mRNA Translation: AAH41638.1
BC093784 mRNA Translation: AAH93784.1
BC103706 mRNA Translation: AAI03707.1
BC111983 mRNA Translation: AAI11984.1
BC143253 mRNA Translation: AAI43254.1
CCDSiCCDS9117.1 [Q15020-1]
RefSeqiNP_055521.1, NM_014706.3 [Q15020-1]
UniGeneiHs.584842

Genome annotation databases

EnsembliENST00000228284; ENSP00000228284; ENSG00000075856 [Q15020-1]
ENST00000431469; ENSP00000414453; ENSG00000075856 [Q15020-4]
ENST00000546611; ENSP00000448554; ENSG00000075856 [Q15020-3]
ENST00000546728; ENSP00000449743; ENSG00000075856 [Q15020-2]
GeneIDi9733
KEGGihsa:9733
UCSCiuc001tmz.2 human [Q15020-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiSART3_HUMAN
AccessioniPrimary (citable) accession number: Q15020
Secondary accession number(s): A8K2E4
, B7ZKM0, Q2M2H0, Q58F06, Q8IUS1, Q96J95
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: November 1, 1996
Last modified: September 12, 2018
This is version 170 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health

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