UniProtKB - Q149S1 (TEKT4_MOUSE)
Protein
Tektin-4
Gene
Tekt4
Organism
Mus musculus (Mouse)
Status
Functioni
May be a structural component of the sperm flagellum. Contributes to normal sperm motility.1 Publication
GO - Biological processi
- cilium assembly Source: GO_Central
- cilium movement involved in cell motility Source: MGI
- regulation of brood size Source: UniProtKB
Keywordsi
Biological process | Cilium biogenesis/degradation |
Names & Taxonomyi
Protein namesi | Recommended name: Tektin-4Alternative name(s): Testicular microtubules-related protein 4 |
Gene namesi | Name:Tekt4 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1919090, Tekt4 |
Subcellular locationi
Other locations
- flagellum 2 Publications
Note: Found in the abaxial (convex) surface of outer dense fibers in sperm flagella.1 Publication
Cytoskeleton
- microtubule cytoskeleton Source: GO_Central
Other locations
- sperm flagellum Source: MGI
- sperm midpiece Source: MGI
- sperm principal piece Source: MGI
Keywords - Cellular componenti
Cell projection, Cilium, FlagellumPathology & Biotechi
Disruption phenotypei
In a 129S5/SvEvBrd genetic background, males show progressive reduction in fertility with almost complete loss of fertility after 5 months of breeding. Testis weight and histology appear normal. Spermatozoa have significantly reduced forward motility. The sperm flagellum shows defective bending in the midpiece region which impairs waveform propagation and forward propulsion. Sperm ATP levels deplete significantly over time, probably as a result of excess energy consumption from inefficient flagellar beating. The ultrastructure of the flagellum has some subtle abnormalities with an enlarged space between the mitochondrial sheath and the outer dense fibers. In a mixed C57BL/6J;129S5/SvEvBrd genetic background, male fertility is not significantly affected.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000261163 | 1 – 447 | Tektin-4Add BLAST | 447 |
Proteomic databases
PaxDbi | Q149S1 |
PRIDEi | Q149S1 |
2D gel databases
REPRODUCTION-2DPAGEi | Q149S1 |
PTM databases
iPTMneti | Q149S1 |
PhosphoSitePlusi | Q149S1 |
Expressioni
Tissue specificityi
Detected in testis, where it is weakly expressed in round spermatids, and strongly expressed in the flagellum of step 16 elongated spermatids (at protein level) (PubMed:17244819). In the sperm flagellum, localizes to the principal piece and midpiece (at protein level) (PubMed:17244819, PubMed:16596631). Specifically expressed in testis; not detected in other tissues tested (PubMed:17244819).2 Publications
Developmental stagei
Detected in testis from postnatal day 16 onwards, reaching maximal levels by postnatal day 18.1 Publication
Gene expression databases
ExpressionAtlasi | Q149S1, baseline and differential |
Interactioni
Protein-protein interaction databases
BioGRIDi | 214969, 1 interactor |
STRINGi | 10090.ENSMUSP00000025002 |
Miscellaneous databases
RNActi | Q149S1, protein |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 322 – 348 | Sequence analysisAdd BLAST | 27 | |
Coiled coili | 375 – 423 | Sequence analysisAdd BLAST | 49 |
Sequence similaritiesi
Belongs to the tektin family.Curated
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG2685, Eukaryota |
GeneTreei | ENSGT00950000182894 |
InParanoidi | Q149S1 |
OMAi | WAKFTQD |
OrthoDBi | 581072at2759 |
PhylomeDBi | Q149S1 |
TreeFami | TF320754 |
Family and domain databases
InterProi | View protein in InterPro IPR000435, Tektin |
PANTHERi | PTHR19960, PTHR19960, 1 hit |
PRINTSi | PR00511, TEKTIN |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q149S1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAQTGVLLTK EPAPQSIDVC ELPRKEYEVA CNTGAYTSSG LATAGFRTAK
60 70 80 90 100
YLRDEWFQNS YARYHQAFAD RDYSERQRHE SGQLVAETGA LAQRTQLDST
110 120 130 140 150
RKVGERLEDM HCWKSELQRE IDELSSETDL MMAQKLRLQR ALDATSVPYS
160 170 180 190 200
IATDNLQCRE RRQHPDLVRD YVEVELLKET ELIRNIQELL KRTIGQAVDQ
210 220 230 240 250
IRLNREHKES CEMNWSDKVE VYNIDDTCSR YTNESTQVQF YPHSSKFEES
260 270 280 290 300
ASTPETWAKF NHDNLLRAER ERLASVNLRK LIDCILRDTA EDLRLQCDAV
310 320 330 340 350
NSAFSSRCQE LDDSLQKLQY HLRKTLTEIT DQEHQIAALK QAIKDKEAPL
360 370 380 390 400
RVAQTRLYQR SHRPNVELCR DNAQFRLLSE VEELNMSLRA LKEKLQDAEQ
410 420 430 440
ALRNLEDSRM SLEKDIAVKT NSLFIDRQKC MTHRNRYPSV LQLAGYQ
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A3Q4EGQ7 | A0A3Q4EGQ7_MOUSE | Tektin | Tekt4 | 383 | Annotation score: | ||
A0A3Q4EH78 | A0A3Q4EH78_MOUSE | Tektin | Tekt4 | 442 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 262 | H → Q in AAI17511 (PubMed:15489334). | 1 | |
Sequence conflicti | 262 | H → Q in AAI17527 (PubMed:15489334). | 1 | |
Sequence conflicti | 434 | R → S in BAB24268 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY485267 mRNA Translation: AAS55789.1 AK005842 mRNA Translation: BAB24268.1 AC131323 Genomic DNA No translation available. BC117510 mRNA Translation: AAI17511.1 BC117526 mRNA Translation: AAI17527.1 |
CCDSi | CCDS28519.1 |
RefSeqi | NP_082227.1, NM_027951.1 |
Genome annotation databases
Ensembli | ENSMUST00000025002; ENSMUSP00000025002; ENSMUSG00000024175 |
GeneIDi | 71840 |
KEGGi | mmu:71840 |
UCSCi | uc008baw.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY485267 mRNA Translation: AAS55789.1 AK005842 mRNA Translation: BAB24268.1 AC131323 Genomic DNA No translation available. BC117510 mRNA Translation: AAI17511.1 BC117526 mRNA Translation: AAI17527.1 |
CCDSi | CCDS28519.1 |
RefSeqi | NP_082227.1, NM_027951.1 |
3D structure databases
SMRi | Q149S1 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 214969, 1 interactor |
STRINGi | 10090.ENSMUSP00000025002 |
PTM databases
iPTMneti | Q149S1 |
PhosphoSitePlusi | Q149S1 |
2D gel databases
REPRODUCTION-2DPAGEi | Q149S1 |
Proteomic databases
PaxDbi | Q149S1 |
PRIDEi | Q149S1 |
Genome annotation databases
Ensembli | ENSMUST00000025002; ENSMUSP00000025002; ENSMUSG00000024175 |
GeneIDi | 71840 |
KEGGi | mmu:71840 |
UCSCi | uc008baw.1, mouse |
Organism-specific databases
CTDi | 150483 |
MGIi | MGI:1919090, Tekt4 |
Phylogenomic databases
eggNOGi | KOG2685, Eukaryota |
GeneTreei | ENSGT00950000182894 |
InParanoidi | Q149S1 |
OMAi | WAKFTQD |
OrthoDBi | 581072at2759 |
PhylomeDBi | Q149S1 |
TreeFami | TF320754 |
Miscellaneous databases
BioGRID-ORCSi | 71840, 0 hits in 17 CRISPR screens |
PROi | PR:Q149S1 |
RNActi | Q149S1, protein |
SOURCEi | Search... |
Gene expression databases
ExpressionAtlasi | Q149S1, baseline and differential |
Family and domain databases
InterProi | View protein in InterPro IPR000435, Tektin |
PANTHERi | PTHR19960, PTHR19960, 1 hit |
PRINTSi | PR00511, TEKTIN |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TEKT4_MOUSE | |
Accessioni | Q149S1Primary (citable) accession number: Q149S1 Secondary accession number(s): A0A0R4J051, Q3ZTL6, Q9DAH3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 28, 2006 |
Last sequence update: | October 10, 2018 | |
Last modified: | December 2, 2020 | |
This is version 89 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families