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Protein

Importin subunit beta-1

Gene

KPNB1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with IPO7 mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Imports SNAI1 and PRKCI into the nucleus.5 Publications

GO - Molecular functioni

  • enzyme binding Source: UniProtKB
  • Hsp90 protein binding Source: Ensembl
  • nuclear localization sequence binding Source: ProtInc
  • protein domain specific binding Source: UniProtKB
  • protein transporter activity Source: GO_Central
  • Ran GTPase binding Source: InterPro
  • RNA binding Source: UniProtKB
  • zinc ion binding Source: ProtInc

GO - Biological processi

Keywordsi

Biological processHost-virus interaction, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-1169408 ISG15 antiviral mechanism
R-HSA-1655829 Regulation of cholesterol biosynthesis by SREBP (SREBF)
R-HSA-168271 Transport of Ribonucleoproteins into the Host Nucleus
R-HSA-168276 NS1 Mediated Effects on Host Pathways
R-HSA-180746 Nuclear import of Rev protein
R-HSA-211227 Activation of DNA fragmentation factor
R-HSA-6798695 Neutrophil degranulation
SignaLinkiQ14974
SIGNORiQ14974

Protein family/group databases

TCDBi1.I.1.1.3 the eukaryotic nuclear pore complex (e-npc) family

Names & Taxonomyi

Protein namesi
Recommended name:
Importin subunit beta-1
Alternative name(s):
Importin-90
Karyopherin subunit beta-1
Nuclear factor p97
Pore targeting complex 97 kDa subunit
Short name:
PTAC97
Gene namesi
Name:KPNB1
Synonyms:NTF97
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000108424.9
HGNCiHGNC:6400 KPNB1
MIMi602738 gene
neXtProtiNX_Q14974

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi178I → A: Largely reduced binding to FxFG repeats and reduced nuclear import. 1 Publication1
Mutagenesisi178I → F or D: Loss of binding to FxFG repeats and reduced nuclear import. 1 Publication1

Organism-specific databases

DisGeNETi3837
OpenTargetsiENSG00000108424
PharmGKBiPA30191

Chemistry databases

ChEMBLiCHEMBL3885569

Polymorphism and mutation databases

DMDMi20981701

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001207451 – 876Importin subunit beta-1Add BLAST876

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1 Publication1
Modified residuei12PhosphoserineCombined sources1
Modified residuei211N6-acetyllysineCombined sources1
Modified residuei835N6-acetyllysineCombined sources1
Modified residuei867N6-acetyllysineCombined sources1

Post-translational modificationi

Mono-ADP-ribosylated by PARP16.

Keywords - PTMi

Acetylation, ADP-ribosylation, Phosphoprotein

Proteomic databases

EPDiQ14974
MaxQBiQ14974
PaxDbiQ14974
PeptideAtlasiQ14974
PRIDEiQ14974
ProteomicsDBi60268
TopDownProteomicsiQ14974-1 [Q14974-1]

PTM databases

iPTMnetiQ14974
PhosphoSitePlusiQ14974
SwissPalmiQ14974

Expressioni

Gene expression databases

BgeeiENSG00000108424 Expressed in 238 organ(s), highest expression level in testis
CleanExiHS_KPNB1
ExpressionAtlasiQ14974 baseline and differential
GenevisibleiQ14974 HS

Organism-specific databases

HPAiCAB034449
HPA029878
HPA050302

Interactioni

Subunit structurei

Forms a complex with an importin alpha subunit (PubMed:8617227, PubMed:8692944). Forms a heterodimer with IPO7. Interacts with IPO7, SNUPN, RPL23A and XPO1. The KPNB1/IPO7 heterodimer interacts with H1 histone. Interacts with H2A, H2B, H3 and H4 histones (By similarity). Component of an import snRNP complex composed of KPNB1, SNUPN, SMN1 and ZNF259. Component of a nuclear export receptor complex composed of KPNB1, Ran, SNUPN and XPO1. Interacts with SRY. Interacts with PRKCI/atypical protein kinase C iota. Interacts with KPNA7. Interacts with SNAI1 (via zinc fingers) and SNAI2 (via zinc fingers). Interacts with SLC35G1 and STIM1. Interacts with DCAF8. Interacts with RAN (By similarity). Interacts with NUMA1 (via C-terminus); this interaction is inhibited by RanGTP (PubMed:11229403).By similarity18 Publications
(Microbial infection) Interacts with HIV-1 Rev and Tat.2 Publications
(Microbial infection) Interacts with HTLV-1 Rex.1 Publication
(Microbial infection) Interacts with human respiratory syncytial virus (HRSV) protein M.2 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi110035, 267 interactors
ComplexPortaliCPX-1027 Importin complex, KPNA2 variant
CPX-1032 Importin complex, Snurportin variant
CPX-1055 Importin complex, KPNA1 variant
CPX-1057 Importin complex, KPNA3 variant
CPX-1060 Importin complex, KPNA4 variant
CPX-1063 Importin complex, KPNA5 variant
CPX-1064 Importin complex, KPNA6 variant
CPX-1066 Importin complex, KPNA7 variant
CORUMiQ14974
DIPiDIP-6204N
IntActiQ14974, 178 interactors
MINTiQ14974
STRINGi9606.ENSP00000290158

Structurei

Secondary structure

1876
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ14974
SMRiQ14974
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ14974

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati2 – 31HEAT 12 PublicationsAdd BLAST30
Domaini21 – 101Importin N-terminalPROSITE-ProRule annotationAdd BLAST81
Repeati33 – 65HEAT 22 PublicationsAdd BLAST33
Repeati85 – 123HEAT 32 PublicationsAdd BLAST39
Repeati129 – 160HEAT 42 PublicationsAdd BLAST32
Repeati170 – 202HEAT 52 PublicationsAdd BLAST33
Repeati212 – 247HEAT 62 PublicationsAdd BLAST36
Repeati253 – 302HEAT 72 PublicationsAdd BLAST50
Repeati314 – 360HEAT 82 PublicationsAdd BLAST47
Repeati364 – 394HEAT 92 PublicationsAdd BLAST31
Repeati402 – 438HEAT 102 PublicationsAdd BLAST37
Repeati449 – 485HEAT 111 PublicationAdd BLAST37
Repeati500 – 537HEAT 121 PublicationAdd BLAST38
Repeati544 – 592HEAT 131 PublicationAdd BLAST49
Repeati600 – 639HEAT 141 PublicationAdd BLAST40
Repeati644 – 681HEAT 151 PublicationAdd BLAST38
Repeati686 – 724HEAT 161 PublicationAdd BLAST39
Repeati732 – 776HEAT 171 PublicationAdd BLAST45
Repeati786 – 829HEAT 181 PublicationAdd BLAST44
Repeati831 – 873HEAT 191 PublicationAdd BLAST43

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni329 – 342IAB-bindingAdd BLAST14
Regioni334 – 419Ran-GTP bindingAdd BLAST86

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi337 – 341Poly-Asp5

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1241 Eukaryota
COG5215 LUCA
GeneTreeiENSGT00550000074898
HOGENOMiHOG000204108
HOVERGENiHBG002369
InParanoidiQ14974
KOiK14293
OMAiKNHLTSK
OrthoDBiEOG091G01XQ
PhylomeDBiQ14974
TreeFamiTF105655

Family and domain databases

Gene3Di1.25.10.10, 1 hit
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR000225 Armadillo
IPR021133 HEAT_type_2
IPR001494 Importin-beta_N
PfamiView protein in Pfam
PF03810 IBN_N, 1 hit
SMARTiView protein in SMART
SM00185 ARM, 3 hits
SM00913 IBN_N, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS50077 HEAT_REPEAT, 1 hit
PS50166 IMPORTIN_B_NT, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q14974-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MELITILEKT VSPDRLELEA AQKFLERAAV ENLPTFLVEL SRVLANPGNS
60 70 80 90 100
QVARVAAGLQ IKNSLTSKDP DIKAQYQQRW LAIDANARRE VKNYVLQTLG
110 120 130 140 150
TETYRPSSAS QCVAGIACAE IPVNQWPELI PQLVANVTNP NSTEHMKEST
160 170 180 190 200
LEAIGYICQD IDPEQLQDKS NEILTAIIQG MRKEEPSNNV KLAATNALLN
210 220 230 240 250
SLEFTKANFD KESERHFIMQ VVCEATQCPD TRVRVAALQN LVKIMSLYYQ
260 270 280 290 300
YMETYMGPAL FAITIEAMKS DIDEVALQGI EFWSNVCDEE MDLAIEASEA
310 320 330 340 350
AEQGRPPEHT SKFYAKGALQ YLVPILTQTL TKQDENDDDD DWNPCKAAGV
360 370 380 390 400
CLMLLATCCE DDIVPHVLPF IKEHIKNPDW RYRDAAVMAF GCILEGPEPS
410 420 430 440 450
QLKPLVIQAM PTLIELMKDP SVVVRDTAAW TVGRICELLP EAAINDVYLA
460 470 480 490 500
PLLQCLIEGL SAEPRVASNV CWAFSSLAEA AYEAADVADD QEEPATYCLS
510 520 530 540 550
SSFELIVQKL LETTDRPDGH QNNLRSSAYE SLMEIVKNSA KDCYPAVQKT
560 570 580 590 600
TLVIMERLQQ VLQMESHIQS TSDRIQFNDL QSLLCATLQN VLRKVQHQDA
610 620 630 640 650
LQISDVVMAS LLRMFQSTAG SGGVQEDALM AVSTLVEVLG GEFLKYMEAF
660 670 680 690 700
KPFLGIGLKN YAEYQVCLAA VGLVGDLCRA LQSNIIPFCD EVMQLLLENL
710 720 730 740 750
GNENVHRSVK PQILSVFGDI ALAIGGEFKK YLEVVLNTLQ QASQAQVDKS
760 770 780 790 800
DYDMVDYLNE LRESCLEAYT GIVQGLKGDQ ENVHPDVMLV QPRVEFILSF
810 820 830 840 850
IDHIAGDEDH TDGVVACAAG LIGDLCTAFG KDVLKLVEAR PMIHELLTEG
860 870
RRSKTNKAKT LATWATKELR KLKNQA
Length:876
Mass (Da):97,170
Last modified:May 15, 2002 - v2
Checksum:iF3BB8B73E7E51639
GO
Isoform 2 (identifier: Q14974-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-145: Missing.

Note: No experimental confirmation available.
Show »
Length:731
Mass (Da):81,179
Checksum:i03F6C311DF096541
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
J3KTM9J3KTM9_HUMAN
Importin subunit beta-1
KPNB1
690Annotation score:
J3QR48J3QR48_HUMAN
Importin subunit beta-1
KPNB1
148Annotation score:
J3QKQ5J3QKQ5_HUMAN
Importin subunit beta-1
KPNB1
248Annotation score:
J3QRG4J3QRG4_HUMAN
Importin subunit beta-1
KPNB1
60Annotation score:
J3KS06J3KS06_HUMAN
Importin subunit beta-1
KPNB1
22Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti97Q → H in AAA82869 (PubMed:7615630).Curated1
Sequence conflicti200N → NA AA sequence (PubMed:8617227).Curated1
Sequence conflicti863T → R in AAA82869 (PubMed:7615630).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0546121 – 145Missing in isoform 2. 1 PublicationAdd BLAST145

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L39793 Genomic DNA Translation: AAA82869.1
L38951 mRNA Translation: AAC41763.1
BT009797 mRNA Translation: AAP88799.1
AK316421 mRNA Translation: BAH14792.1
AC015674 Genomic DNA No translation available.
AC025682 Genomic DNA No translation available.
CH471109 Genomic DNA Translation: EAW94807.1
CH471109 Genomic DNA Translation: EAW94808.1
CH471109 Genomic DNA Translation: EAW94810.1
BC003572 mRNA Translation: AAH03572.1
BC024045 mRNA Translation: AAH24045.1
BC036703 mRNA Translation: AAH36703.1
CCDSiCCDS11513.1 [Q14974-1]
CCDS62228.1 [Q14974-2]
PIRiI52907
RefSeqiNP_001263382.1, NM_001276453.1 [Q14974-2]
NP_002256.2, NM_002265.5 [Q14974-1]
UniGeneiHs.532793

Genome annotation databases

EnsembliENST00000290158; ENSP00000290158; ENSG00000108424 [Q14974-1]
ENST00000535458; ENSP00000438253; ENSG00000108424 [Q14974-2]
ENST00000540627; ENSP00000438964; ENSG00000108424 [Q14974-2]
GeneIDi3837
KEGGihsa:3837
UCSCiuc002ilt.3 human [Q14974-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L39793 Genomic DNA Translation: AAA82869.1
L38951 mRNA Translation: AAC41763.1
BT009797 mRNA Translation: AAP88799.1
AK316421 mRNA Translation: BAH14792.1
AC015674 Genomic DNA No translation available.
AC025682 Genomic DNA No translation available.
CH471109 Genomic DNA Translation: EAW94807.1
CH471109 Genomic DNA Translation: EAW94808.1
CH471109 Genomic DNA Translation: EAW94810.1
BC003572 mRNA Translation: AAH03572.1
BC024045 mRNA Translation: AAH24045.1
BC036703 mRNA Translation: AAH36703.1
CCDSiCCDS11513.1 [Q14974-1]
CCDS62228.1 [Q14974-2]
PIRiI52907
RefSeqiNP_001263382.1, NM_001276453.1 [Q14974-2]
NP_002256.2, NM_002265.5 [Q14974-1]
UniGeneiHs.532793

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1F59X-ray2.80A/B1-442[»]
1IBRX-ray2.30B/D1-462[»]
1M5NX-ray2.90S1-485[»]
1O6OX-ray2.80A/B/C1-442[»]
1O6PX-ray2.80A/B1-442[»]
1QGKX-ray2.50A1-876[»]
1QGRX-ray2.30A1-876[»]
2P8QX-ray2.35A1-876[»]
2Q5DX-ray3.20A/B1-876[»]
2QNAX-ray2.84A127-875[»]
3LWWX-ray3.15A/C1-876[»]
3W5KX-ray2.60A1-876[»]
ProteinModelPortaliQ14974
SMRiQ14974
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110035, 267 interactors
ComplexPortaliCPX-1027 Importin complex, KPNA2 variant
CPX-1032 Importin complex, Snurportin variant
CPX-1055 Importin complex, KPNA1 variant
CPX-1057 Importin complex, KPNA3 variant
CPX-1060 Importin complex, KPNA4 variant
CPX-1063 Importin complex, KPNA5 variant
CPX-1064 Importin complex, KPNA6 variant
CPX-1066 Importin complex, KPNA7 variant
CORUMiQ14974
DIPiDIP-6204N
IntActiQ14974, 178 interactors
MINTiQ14974
STRINGi9606.ENSP00000290158

Chemistry databases

ChEMBLiCHEMBL3885569

Protein family/group databases

TCDBi1.I.1.1.3 the eukaryotic nuclear pore complex (e-npc) family

PTM databases

iPTMnetiQ14974
PhosphoSitePlusiQ14974
SwissPalmiQ14974

Polymorphism and mutation databases

DMDMi20981701

Proteomic databases

EPDiQ14974
MaxQBiQ14974
PaxDbiQ14974
PeptideAtlasiQ14974
PRIDEiQ14974
ProteomicsDBi60268
TopDownProteomicsiQ14974-1 [Q14974-1]

Protocols and materials databases

DNASUi3837
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000290158; ENSP00000290158; ENSG00000108424 [Q14974-1]
ENST00000535458; ENSP00000438253; ENSG00000108424 [Q14974-2]
ENST00000540627; ENSP00000438964; ENSG00000108424 [Q14974-2]
GeneIDi3837
KEGGihsa:3837
UCSCiuc002ilt.3 human [Q14974-1]

Organism-specific databases

CTDi3837
DisGeNETi3837
EuPathDBiHostDB:ENSG00000108424.9
GeneCardsiKPNB1
HGNCiHGNC:6400 KPNB1
HPAiCAB034449
HPA029878
HPA050302
MIMi602738 gene
neXtProtiNX_Q14974
OpenTargetsiENSG00000108424
PharmGKBiPA30191
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1241 Eukaryota
COG5215 LUCA
GeneTreeiENSGT00550000074898
HOGENOMiHOG000204108
HOVERGENiHBG002369
InParanoidiQ14974
KOiK14293
OMAiKNHLTSK
OrthoDBiEOG091G01XQ
PhylomeDBiQ14974
TreeFamiTF105655

Enzyme and pathway databases

ReactomeiR-HSA-1169408 ISG15 antiviral mechanism
R-HSA-1655829 Regulation of cholesterol biosynthesis by SREBP (SREBF)
R-HSA-168271 Transport of Ribonucleoproteins into the Host Nucleus
R-HSA-168276 NS1 Mediated Effects on Host Pathways
R-HSA-180746 Nuclear import of Rev protein
R-HSA-211227 Activation of DNA fragmentation factor
R-HSA-6798695 Neutrophil degranulation
SignaLinkiQ14974
SIGNORiQ14974

Miscellaneous databases

ChiTaRSiKPNB1 human
EvolutionaryTraceiQ14974
GeneWikiiKPNB1
GenomeRNAii3837
PROiPR:Q14974
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000108424 Expressed in 238 organ(s), highest expression level in testis
CleanExiHS_KPNB1
ExpressionAtlasiQ14974 baseline and differential
GenevisibleiQ14974 HS

Family and domain databases

Gene3Di1.25.10.10, 1 hit
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR000225 Armadillo
IPR021133 HEAT_type_2
IPR001494 Importin-beta_N
PfamiView protein in Pfam
PF03810 IBN_N, 1 hit
SMARTiView protein in SMART
SM00185 ARM, 3 hits
SM00913 IBN_N, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS50077 HEAT_REPEAT, 1 hit
PS50166 IMPORTIN_B_NT, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiIMB1_HUMAN
AccessioniPrimary (citable) accession number: Q14974
Secondary accession number(s): B7ZAV6
, D3DTT3, Q14637, Q53XN2, Q96J27
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 15, 2002
Last modified: October 10, 2018
This is version 205 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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