UniProtKB - Q148W0 (AT8B1_MOUSE)
Phospholipid-transporting ATPase IC
Atp8b1
Functioni
Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of phospholipids, in particular phosphatidylcholines (PC), from the outer to the inner leaflet of the plasma membrane (By similarity).
May participate in the establishment of the canalicular membrane integrity by ensuring asymmetric distribution of phospholipids in the canicular membrane (PubMed:21820390).
Thus may have a role in the regulation of bile acids transport into the canaliculus, uptake of bile acids from intestinal contents into intestinal mucosa or both and protect hepatocytes from bile salts (PubMed:14976163, PubMed:21820390, PubMed:20126555).
Involved in the microvillus formation in polarized epithelial cells; the function seems to be independent from its flippase activity (By similarity).
Participates in correct apical membrane localization of CDC42, CFTR and SLC10A2 (PubMed:26416959).
Enables CDC42 clustering at the apical membrane during enterocyte polarization through the interaction between CDC42 polybasic region and negatively charged membrane lipids provided by ATP8B1 (PubMed:26416959).
Together with TMEM30A is involved in uptake of the synthetic drug alkylphospholipid perifosine (By similarity).
Required for the preservation of cochlear hair cells in the inner ear (PubMed:19478059).
According PubMed:20852622 is proposed to act as cardiolipin transporter during inflammatory injury; the function is questioned by PubMed:21475228 (PubMed:20852622, PubMed:21475228).
By similarity7 PublicationsCatalytic activityi
- a 1,2-diacyl-sn-glycero-3-phosphocholine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphocholine(in) + ADP + H+ + phosphateBy similarityThis reaction proceeds in the forwardBy similarity direction.
- a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) + ADP + H+ + phosphateBy similarityThis reaction proceeds in the forwardBy similarity direction.
- ATP + H(2)O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2).By similarity EC:7.6.2.1
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 454 | 4-aspartylphosphate intermediateBy similarity | 1 | |
Metal bindingi | 893 | MagnesiumBy similarity | 1 | |
Metal bindingi | 897 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- aminophospholipid flippase activity Source: BHF-UCL
- ATPase-coupled intramembrane lipid transporter activity Source: GO_Central
- ATP binding Source: UniProtKB-KW
- ATP hydrolysis activity Source: InterPro
- cardiolipin binding Source: UniProtKB
- lipid transporter activity Source: MGI
- magnesium ion binding Source: InterPro
- phosphatidylcholine flippase activity Source: MGI
- phosphatidylcholine floppase activity Source: RHEA
- phosphatidylserine flippase activity Source: UniProtKB
- phosphatidylserine floppase activity Source: RHEA
GO - Biological processi
- aminophospholipid transport Source: BHF-UCL
- apical protein localization Source: UniProtKB
- bile acid and bile salt transport Source: UniProtKB
- bile acid metabolic process Source: MGI
- Golgi organization Source: GO_Central
- inner ear receptor cell development Source: UniProtKB
- negative regulation of transcription, DNA-templated Source: MGI
- organic anion transport Source: UniProtKB
- phospholipid translocation Source: MGI
- regulation of chloride transport Source: UniProtKB
- regulation of microvillus assembly Source: MGI
- regulation of plasma membrane organization Source: UniProtKB
- sensory perception of sound Source: UniProtKB
- vestibulocochlear nerve formation Source: UniProtKB
- xenobiotic transmembrane transport Source: MGI
Keywordsi
Molecular function | Translocase |
Biological process | Hearing, Lipid transport, Transport |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 7.6.2.1, 3474 |
Reactomei | R-MMU-936837, Ion transport by P-type ATPases |
Names & Taxonomyi
Protein namesi | Recommended name: Phospholipid-transporting ATPase ICCurated (EC:7.6.2.1By similarity)Alternative name(s): ATPase class I type 8B member 1 P4-ATPase flippase complex alpha subunit ATP8B1 |
Gene namesi | Name:Atp8b1Imported |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1859665, Atp8b1 |
VEuPathDBi | HostDB:ENSMUSG00000039529 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
- Apical cell membrane 1 Publication
Golgi apparatus
- Golgi apparatus By similarity
Endoplasmic reticulum
- Endoplasmic reticulum By similarity
Other locations
- stereocilium 1 Publication
Note: Exit from the endoplasmic reticulum requires the presence of TMEM30A or TMEM30B. Localizes to apical membranes in epithelial cells.By similarity
Cytosol
- cytosol Source: MGI
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
Golgi apparatus
- Golgi apparatus Source: MGI
- trans-Golgi network Source: GO_Central
Nucleus
- nuclear body Source: MGI
- nucleoplasm Source: MGI
Plasma Membrane
- apical plasma membrane Source: MGI
- brush border membrane Source: MGI
- integral component of plasma membrane Source: MGI
- plasma membrane Source: BHF-UCL
Other locations
- membrane Source: MGI
- phospholipid-translocating ATPase complex Source: UniProtKB
- stereocilium Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 121 | CytoplasmicSequence analysisAdd BLAST | 121 | |
Transmembranei | 122 – 142 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 143 – 144 | Exoplasmic loopSequence analysis | 2 | |
Transmembranei | 145 – 165 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 166 – 339 | CytoplasmicSequence analysisAdd BLAST | 174 | |
Transmembranei | 340 – 360 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 361 – 385 | Exoplasmic loopSequence analysisAdd BLAST | 25 | |
Transmembranei | 386 – 406 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 407 – 952 | CytoplasmicSequence analysisAdd BLAST | 546 | |
Transmembranei | 953 – 973 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 974 – 982 | Exoplasmic loopSequence analysis | 9 | |
Transmembranei | 983 – 1003 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1004 – 1032 | CytoplasmicSequence analysisAdd BLAST | 29 | |
Transmembranei | 1033 – 1053 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1054 – 1071 | Exoplasmic loopSequence analysisAdd BLAST | 18 | |
Transmembranei | 1072 – 1092 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1093 – 1094 | CytoplasmicSequence analysis | 2 | |
Transmembranei | 1095 – 1115 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1116 – 1142 | Exoplasmic loopSequence analysisAdd BLAST | 27 | |
Transmembranei | 1143 – 1163 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1164 – 1251 | CytoplasmicSequence analysisAdd BLAST | 88 |
Keywords - Cellular componenti
Cell membrane, Cell projection, Endoplasmic reticulum, Golgi apparatus, MembranePathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 308 | G → V: Markly decreased expression, hearing loss associated with degeneration of cochlear hair cells and spiral ganglion cells. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000370862 | 1 – 1251 | Phospholipid-transporting ATPase ICAdd BLAST | 1251 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1223 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | Q148W0 |
PaxDbi | Q148W0 |
PeptideAtlasi | Q148W0 |
PRIDEi | Q148W0 |
ProteomicsDBi | 265134 |
PTM databases
iPTMneti | Q148W0 |
PhosphoSitePlusi | Q148W0 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000039529, Expressed in intestine and 190 other tissues |
ExpressionAtlasi | Q148W0, baseline and differential |
Genevisiblei | Q148W0, MM |
Interactioni
Subunit structurei
Component of a P4-ATPase flippase complex which consists of a catalytic alpha subunit ATP8B1 and an accessory beta subunit TMEM30A (PubMed:30018401). The flippase ATP8B1:TMEM30A complex can form an intermediate phosphoenzyme in vitro.
Also interacts with beta subunit TMEM30B.
By similarity1 PublicationProtein-protein interaction databases
BioGRIDi | 207714, 1 interactor |
STRINGi | 10090.ENSMUSP00000025482 |
Miscellaneous databases
RNActi | Q148W0, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 52 | DisorderedSequence analysisAdd BLAST | 52 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 13 – 38 | Acidic residuesSequence analysisAdd BLAST | 26 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0206, Eukaryota |
GeneTreei | ENSGT00940000158002 |
HOGENOMi | CLU_000846_3_2_1 |
InParanoidi | Q148W0 |
OMAi | VQEPFFP |
OrthoDBi | 587717at2759 |
PhylomeDBi | Q148W0 |
TreeFami | TF300654 |
Family and domain databases
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR030346, ATP8B1 IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR006539, P-type_ATPase_IV IPR032631, P-type_ATPase_N IPR001757, P_typ_ATPase IPR032630, P_typ_ATPase_c IPR044492, P_typ_ATPase_HD_dom |
PANTHERi | PTHR24092:SF48, PTHR24092:SF48, 1 hit |
Pfami | View protein in Pfam PF16212, PhoLip_ATPase_C, 1 hit PF16209, PhoLip_ATPase_N, 1 hit |
SFLDi | SFLDF00027, p-type_atpase, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81660, SSF81660, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01652, ATPase-Plipid, 1 hit TIGR01494, ATPase_P-type, 1 hit |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MSTERDSETT FDEESQPNDE VVPYSDDETE DELEDQGSTV EPEQNRVNRE
60 70 80 90 100
AEKKRETFRK DCTWQVKAND RKFHEQPHFM NTKFFCIKES KYASNAIKTY
110 120 130 140 150
KYNGFTFLPM NLFEQFKRAA NFYFLILLIL QAIPQISTLA WYTTLVPLLL
160 170 180 190 200
VLGITAIKDL VDDVARHKMD KEINNRTCEV IKDGRFKIIK WKDIQVGDVI
210 220 230 240 250
RLKKNDFIPA DILLLSSSEP NSLCYVETAE LDGETNLKFK MALEITDQYL
260 270 280 290 300
QIEDNLATFD GFIECEEPNN RLDKFTGTLF WKNQSFPLDA DKILLRGCVI
310 320 330 340 350
RNTDVCHGLV IFAGADTKIM KNSGKTRFKR TKIDYLMNYM VYTIFIVLIL
360 370 380 390 400
VSAGLAIGHA YWEAQVGNYS WYLYDGENAT PSYRGFLNFW GYIIVLNTMV
410 420 430 440 450
PISLYVSVEV IRLGQSHFIN WDLQMYYAEK DTPAKARTTT LNEQLGQIHY
460 470 480 490 500
IFSDKTGTLT QNIMTFKKCC INGTIYGDHR DASQHSHSKI ELVDFSWNTF
510 520 530 540 550
ADGKLAFYDH YLIEQIQSGK EPEVRQFFFL LSICHTVMVD RIDGQINYQA
560 570 580 590 600
ASPDEGALVN AARNFGFAFL ARTQNTITVS ELGSERTYNV LAILDFNSDR
610 620 630 640 650
KRMSIIVRTP EGSIRLYCKG ADTVIYERLH RMNPTKQETQ DALDIFASET
660 670 680 690 700
LRTLCLCYKE IEEKEFTEWN NKFMAASVAS SNRDEALDKV YEEIEKDLIL
710 720 730 740 750
LGATAIEDKL QDGVPETISK LAKADIKIWV LTGDKKETAE NIGFACELLT
760 770 780 790 800
EDTTICYGED INSLLHTRME NQRNRGGVSA KFAPPVYEPF FPPGENRALI
810 820 830 840 850
ITGSWLNEIL LEKKTKRSKI LKLKFPRTEE ERRMRSQSRR RLEEKKEQRQ
860 870 880 890 900
KNFVDLACEC SAVICCRVTP KQKAMVVDLV KRYKKAITLA IGDGANDVNM
910 920 930 940 950
IKTAHIGVGI SGQEGMQAVM SSDYSFAQFR YLQRLLLVHG RWSYIRMCKF
960 970 980 990 1000
LRYFFYKNFA FTLVHFWYSF FNGYSAQTAY EDWFITLYNV LYSSLPVLLM
1010 1020 1030 1040 1050
GLLDQDVSDK LSLRFPGLYV VGQRDLLFNY KRFFVSLLHG VLTSMVLFFI
1060 1070 1080 1090 1100
PLGAYLQTVG QDGEAPSDYQ SFAVTVASAL VITVNFQIGL DTSYWTFVNA
1110 1120 1130 1140 1150
FSIFGSIALY FGIMFDFHSA GIHVLFPSAF QFTGTASNAL RQPYIWLTII
1160 1170 1180 1190 1200
LTVAVCLLPV VAIRFLSMTI WPSESDKIQK HRKRLKAEEQ WKRRQSVFRR
1210 1220 1230 1240 1250
GVSSRRSAYA FSHQRGYADL ISSGRSIRKK RSPLDAIIAD GTAEYRRTVE
S
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A494BB33 | A0A494BB33_MOUSE | Phospholipid-transporting ATPase IC | Atp8b1 | 245 | Annotation score: | ||
A0A494B9Z8 | A0A494B9Z8_MOUSE | Phospholipid-transporting ATPase IC | Atp8b1 | 61 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 104 | G → A in AAR90342 (PubMed:14976163).Curated | 1 | |
Sequence conflicti | 108 | L → I in AAR90342 (PubMed:14976163).Curated | 1 | |
Sequence conflicti | 155 | T → M in BAE34046 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 174 | N → S in AAR90342 (PubMed:14976163).Curated | 1 | |
Sequence conflicti | 176 | R → M in AAR90342 (PubMed:14976163).Curated | 1 | |
Sequence conflicti | 531 | L → P in BAE34046 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 575 | N → Y in BAE34046 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 667 | T → A in AAI17947 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 671 | N → K in AAI17947 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 893 | D → E in BAE34046 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 1226 | S → C in BAE34046 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY506548 mRNA Translation: AAR90342.1 AK157316 mRNA Translation: BAE34046.1 BC117946 mRNA Translation: AAI17947.1 |
CCDSi | CCDS29304.1 |
RefSeqi | NP_001001488.2, NM_001001488.3 |
Genome annotation databases
Ensembli | ENSMUST00000025482; ENSMUSP00000025482; ENSMUSG00000039529 |
GeneIDi | 54670 |
KEGGi | mmu:54670 |
UCSCi | uc008fem.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY506548 mRNA Translation: AAR90342.1 AK157316 mRNA Translation: BAE34046.1 BC117946 mRNA Translation: AAI17947.1 |
CCDSi | CCDS29304.1 |
RefSeqi | NP_001001488.2, NM_001001488.3 |
3D structure databases
AlphaFoldDBi | Q148W0 |
SMRi | Q148W0 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 207714, 1 interactor |
STRINGi | 10090.ENSMUSP00000025482 |
PTM databases
iPTMneti | Q148W0 |
PhosphoSitePlusi | Q148W0 |
Proteomic databases
MaxQBi | Q148W0 |
PaxDbi | Q148W0 |
PeptideAtlasi | Q148W0 |
PRIDEi | Q148W0 |
ProteomicsDBi | 265134 |
Protocols and materials databases
Antibodypediai | 9722, 48 antibodies from 12 providers |
DNASUi | 54670 |
Genome annotation databases
Ensembli | ENSMUST00000025482; ENSMUSP00000025482; ENSMUSG00000039529 |
GeneIDi | 54670 |
KEGGi | mmu:54670 |
UCSCi | uc008fem.1, mouse |
Organism-specific databases
CTDi | 5205 |
MGIi | MGI:1859665, Atp8b1 |
VEuPathDBi | HostDB:ENSMUSG00000039529 |
Phylogenomic databases
eggNOGi | KOG0206, Eukaryota |
GeneTreei | ENSGT00940000158002 |
HOGENOMi | CLU_000846_3_2_1 |
InParanoidi | Q148W0 |
OMAi | VQEPFFP |
OrthoDBi | 587717at2759 |
PhylomeDBi | Q148W0 |
TreeFami | TF300654 |
Enzyme and pathway databases
BRENDAi | 7.6.2.1, 3474 |
Reactomei | R-MMU-936837, Ion transport by P-type ATPases |
Miscellaneous databases
BioGRID-ORCSi | 54670, 3 hits in 74 CRISPR screens |
PROi | PR:Q148W0 |
RNActi | Q148W0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000039529, Expressed in intestine and 190 other tissues |
ExpressionAtlasi | Q148W0, baseline and differential |
Genevisiblei | Q148W0, MM |
Family and domain databases
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR030346, ATP8B1 IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR006539, P-type_ATPase_IV IPR032631, P-type_ATPase_N IPR001757, P_typ_ATPase IPR032630, P_typ_ATPase_c IPR044492, P_typ_ATPase_HD_dom |
PANTHERi | PTHR24092:SF48, PTHR24092:SF48, 1 hit |
Pfami | View protein in Pfam PF16212, PhoLip_ATPase_C, 1 hit PF16209, PhoLip_ATPase_N, 1 hit |
SFLDi | SFLDF00027, p-type_atpase, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81660, SSF81660, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01652, ATPase-Plipid, 1 hit TIGR01494, ATPase_P-type, 1 hit |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | AT8B1_MOUSE | |
Accessioni | Q148W0Primary (citable) accession number: Q148W0 Secondary accession number(s): Q3U010, Q6R964 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 5, 2009 |
Last sequence update: | May 5, 2009 | |
Last modified: | May 25, 2022 | |
This is version 130 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families