UniProtKB - Q14865 (ARI5B_HUMAN)
AT-rich interactive domain-containing protein 5B
ARID5B
Functioni
Transcription coactivator that binds to the 5'-AATA[CT]-3' core sequence and plays a key role in adipogenesis and liver development. Acts by forming a complex with phosphorylated PHF2, which mediates demethylation at Lys-336, leading to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. Required for adipogenesis: regulates triglyceride metabolism in adipocytes by regulating expression of adipogenic genes. Overexpression leads to induction of smooth muscle marker genes, suggesting that it may also act as a regulator of smooth muscle cell differentiation and proliferation. Represses the cytomegalovirus enhancer.
1 PublicationGO - Molecular functioni
- DNA binding Source: GDB
- transcription cis-regulatory region binding Source: UniProtKB
- transcription coactivator activity Source: UniProtKB
GO - Biological processi
- adipose tissue development Source: UniProtKB
- adrenal gland development Source: Ensembl
- cell development Source: Ensembl
- cellular response to leukemia inhibitory factor Source: Ensembl
- face morphogenesis Source: Ensembl
- fat cell differentiation Source: Ensembl
- fat pad development Source: Ensembl
- female gonad development Source: Ensembl
- fibroblast migration Source: Ensembl
- kidney development Source: Ensembl
- liver development Source: UniProtKB
- male gonad development Source: Ensembl
- multicellular organism growth Source: Ensembl
- muscle organ morphogenesis Source: Ensembl
- negative regulation of transcription, DNA-templated Source: GDB
- negative regulation of transcription by RNA polymerase II Source: NTNU_SB
- nitrogen compound metabolic process Source: Ensembl
- platelet-derived growth factor receptor signaling pathway Source: Ensembl
- positive regulation of DNA-binding transcription factor activity Source: UniProtKB
- post-embryonic development Source: Ensembl
- regulation of transcription by RNA polymerase II Source: GO_Central
- roof of mouth development Source: Ensembl
- skeletal system morphogenesis Source: Ensembl
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | Q14865 |
Reactomei | R-HSA-3214842, HDMs demethylate histones |
SignaLinki | Q14865 |
SIGNORi | Q14865 |
Names & Taxonomyi
Protein namesi | Recommended name: AT-rich interactive domain-containing protein 5BShort name: ARID domain-containing protein 5B Alternative name(s): MRF1-like protein Modulator recognition factor 2 Short name: MRF-2 |
Gene namesi | Name:ARID5B Synonyms:DESRT, MRF2 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:17362, ARID5B |
MIMi | 608538, gene |
neXtProti | NX_Q14865 |
VEuPathDBi | HostDB:ENSG00000150347 |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation
Nucleus
- nucleoplasm Source: Reactome
- nucleus Source: GO_Central
Keywords - Cellular componenti
NucleusPathology & Biotechi
Involvement in diseasei
Leukemia, acute lymphoblastic (ALL)7 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 336 | K → A or R: Abolishes methylation and FSK-dependent DNA-binding of the PHF2-ARID5B complex to promoters. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 84159 |
MIMi | 613065, phenotype |
OpenTargetsi | ENSG00000150347 |
PharmGKBi | PA134943193 |
Miscellaneous databases
Pharosi | Q14865, Tbio |
Genetic variation databases
BioMutai | ARID5B |
DMDMi | 209572763 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000200581 | 1 – 1188 | AT-rich interactive domain-containing protein 5BAdd BLAST | 1188 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 130 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 264 | PhosphoserineCombined sources | 1 | |
Modified residuei | 336 | N6,N6-dimethyllysine1 Publication | 1 | |
Cross-linki | 445 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 494 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 496 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 767 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 774 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 803 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 810 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 893 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 916 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 920 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 935 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 988 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 1000 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 1013 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 1032 | PhosphoserineCombined sources | 1 | |
Cross-linki | 1055 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 1070 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 1133 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q14865 |
jPOSTi | Q14865 |
MassIVEi | Q14865 |
MaxQBi | Q14865 |
PaxDbi | Q14865 |
PeptideAtlasi | Q14865 |
PRIDEi | Q14865 |
ProteomicsDBi | 60211 [Q14865-1] 60212 [Q14865-2] 60213 [Q14865-3] |
PTM databases
iPTMneti | Q14865 |
PhosphoSitePlusi | Q14865 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000150347, Expressed in uterine cervix and 258 other tissues |
ExpressionAtlasi | Q14865, baseline and differential |
Genevisiblei | Q14865, HS |
Organism-specific databases
HPAi | ENSG00000150347, Tissue enhanced (skeletal) |
Interactioni
Binary interactionsi
Q14865
With | #Exp. | IntAct |
---|---|---|
HDAC1 [Q13547] | 6 | EBI-1210388,EBI-301834 |
Protein-protein interaction databases
BioGRIDi | 123918, 50 interactors |
IntActi | Q14865, 25 interactors |
MINTi | Q14865 |
STRINGi | 9606.ENSP00000279873 |
Miscellaneous databases
RNActi | Q14865, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | Q14865 |
SMRi | Q14865 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q14865 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 318 – 410 | ARIDPROSITE-ProRule annotationAdd BLAST | 93 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 251 – 277 | DisorderedSequence analysisAdd BLAST | 27 | |
Regioni | 412 – 611 | DisorderedSequence analysisAdd BLAST | 200 | |
Regioni | 846 – 874 | DisorderedSequence analysisAdd BLAST | 29 | |
Regioni | 891 – 918 | DisorderedSequence analysisAdd BLAST | 28 | |
Regioni | 956 – 978 | DisorderedSequence analysisAdd BLAST | 23 | |
Regioni | 1028 – 1070 | DisorderedSequence analysisAdd BLAST | 43 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 412 – 479 | Basic and acidic residuesSequence analysisAdd BLAST | 68 | |
Compositional biasi | 509 – 534 | Basic and acidic residuesSequence analysisAdd BLAST | 26 | |
Compositional biasi | 559 – 609 | Polar residuesSequence analysisAdd BLAST | 51 | |
Compositional biasi | 1038 – 1055 | Basic and acidic residuesSequence analysisAdd BLAST | 18 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2744, Eukaryota |
GeneTreei | ENSGT00940000161078 |
HOGENOMi | CLU_007985_0_0_1 |
InParanoidi | Q14865 |
OMAi | SCPQRRD |
OrthoDBi | 368297at2759 |
PhylomeDBi | Q14865 |
TreeFami | TF324725 |
Family and domain databases
Gene3Di | 1.10.150.60, 1 hit |
InterProi | View protein in InterPro IPR030408, ARID5B IPR001606, ARID_dom IPR036431, ARID_dom_sf |
PANTHERi | PTHR13964:SF37, PTHR13964:SF37, 1 hit |
Pfami | View protein in Pfam PF01388, ARID, 1 hit |
SMARTi | View protein in SMART SM00501, BRIGHT, 1 hit |
SUPFAMi | SSF46774, SSF46774, 1 hit |
PROSITEi | View protein in PROSITE PS51011, ARID, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEPNSLQWVG SPCGLHGPYI FYKAFQFHLE GKPRILSLGD FFFVRCTPKD
60 70 80 90 100
PICIAELQLL WEERTSRQLL SSSKLYFLPE DTPQGRNSDH GEDEVIAVSE
110 120 130 140 150
KVIVKLEDLV KWVHSDFSKW RCGFHAGPVK TEALGRNGQK EALLKYRQST
160 170 180 190 200
LNSGLNFKDV LKEKADLGED EEETNVIVLS YPQYCRYRSM LKRIQDKPSS
210 220 230 240 250
ILTDQFALAL GGIAVVSRNP QILYCRDTFD HPTLIENESI CDEFAPNLKG
260 270 280 290 300
RPRKKKPCPQ RRDSFSGVKD SNNNSDGKAV AKVKCEARSA LTKPKNNHNC
310 320 330 340 350
KKVSNEEKPK VAIGEECRAD EQAFLVALYK YMKERKTPIE RIPYLGFKQI
360 370 380 390 400
NLWTMFQAAQ KLGGYETITA RRQWKHIYDE LGGNPGSTSA ATCTRRHYER
410 420 430 440 450
LILPYERFIK GEEDKPLPPI KPRKQENSSQ ENENKTKVSG TKRIKHEIPK
460 470 480 490 500
SKKEKENAPK PQDAAEVSSE QEKEQETLIS QKSIPEPLPA ADMKKKIEGY
510 520 530 540 550
QEFSAKPLAS RVDPEKDNET DQGSNSEKVA EEAGEKGPTP PLPSAPLAPE
560 570 580 590 600
KDSALVPGAS KQPLTSPSAL VDSKQESKLC CFTESPESEP QEASFPSFPT
610 620 630 640 650
TQPPLANQNE TEDDKLPAMA DYIANCTVKV DQLGSDDIHN ALKQTPKVLV
660 670 680 690 700
VQSFDMFKDK DLTGPMNENH GLNYTPLLYS RGNPGIMSPL AKKKLLSQVS
710 720 730 740 750
GASLSSSYPY GSPPPLISKK KLIARDDLCS SLSQTHHGQS TDHMAVSRPS
760 770 780 790 800
VIQHVQSFRS KPSEERKTIN DIFKHEKLSR SDPHRCSFSK HHLNPLADSY
810 820 830 840 850
VLKQEIQEGK DKLLEKRALP HSHMPSFLAD FYSSPHLHSL YRHTEHHLHN
860 870 880 890 900
EQTSKYPSRD MYRESENSSF PSHRHQEKLH VNYLTSLHLQ DKKSAAAEAP
910 920 930 940 950
TDDQPTDLSL PKNPHKPTGK VLGLAHSTTG PQESKGISQF QVLGSQSRDC
960 970 980 990 1000
HPKACRVSPM TMSGPKKYPE SLSRSGKPHH VRLENFRKME GMVHPILHRK
1010 1020 1030 1040 1050
MSPQNIGAAR PIKRSLEDLD LVIAGKKARA VSPLDPSKEV SGKEKASEQE
1060 1070 1080 1090 1100
SEGSKAAHGG HSGGGSEGHK LPLSSPIFPG LYSGSLCNSG LNSRLPAGYS
1110 1120 1130 1140 1150
HSLQYLKNQT VLSPLMQPLA FHSLVMQRGI FTSPTNSQQL YRHLAAATPV
1160 1170 1180
GSSYGDLLHN SIYPLAAINP QAAFPSSQLS SVHPSTKL
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A7P0TAD2 | A0A7P0TAD2_HUMAN | AT-rich interactive domain-containi... | ARID5B | 1,180 | Annotation score: | ||
A0A2R8Y5F2 | A0A2R8Y5F2_HUMAN | AT-rich interactive domain-containi... | ARID5B | 254 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 401 | L → S in AAH15120 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 451 | S → N in AAH15120 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 827 | F → L in CAE46013 (PubMed:17974005).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_009355 | 1 – 243 | Missing in isoform 2. 2 PublicationsAdd BLAST | 243 | |
Alternative sequenceiVSP_009356 | 244 | F → M in isoform 2. 2 Publications | 1 | |
Alternative sequenceiVSP_041560 | 284 – 318 | KCEAR…GEECR → RSFTPRYSFRCIFLFLLFLF ISLCLCLGGSFQFSI in isoform 3. CuratedAdd BLAST | 35 | |
Alternative sequenceiVSP_041561 | 319 – 1188 | Missing in isoform 3. CuratedAdd BLAST | 870 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC067742 Genomic DNA No translation available. AL671972 Genomic DNA No translation available. AK024803 mRNA Translation: BAB15012.1 Sequence problems. AK296921 mRNA Translation: BAG59475.1 BC015120 mRNA Translation: AAH15120.1 Sequence problems. BC036831 mRNA Translation: AAH36831.1 Different initiation. BC066345 mRNA Translation: AAH66345.1 Sequence problems. BC107800 mRNA Translation: AAI07801.1 Sequence problems. M73837 mRNA Translation: AAA59870.1 BX537690 mRNA Translation: CAD97814.1 BX641020 mRNA Translation: CAE46013.1 |
CCDSi | CCDS31208.1 [Q14865-1] CCDS58082.1 [Q14865-2] |
PIRi | S27963 |
RefSeqi | NP_001231567.1, NM_001244638.1 [Q14865-2] NP_115575.1, NM_032199.2 [Q14865-1] |
Genome annotation databases
Ensembli | ENST00000279873; ENSP00000279873; ENSG00000150347 ENST00000309334; ENSP00000308862; ENSG00000150347 [Q14865-2] |
GeneIDi | 84159 |
KEGGi | hsa:84159 |
MANE-Selecti | ENST00000279873.12; ENSP00000279873.7; NM_032199.3; NP_115575.1 |
UCSCi | uc001jlt.3, human [Q14865-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC067742 Genomic DNA No translation available. AL671972 Genomic DNA No translation available. AK024803 mRNA Translation: BAB15012.1 Sequence problems. AK296921 mRNA Translation: BAG59475.1 BC015120 mRNA Translation: AAH15120.1 Sequence problems. BC036831 mRNA Translation: AAH36831.1 Different initiation. BC066345 mRNA Translation: AAH66345.1 Sequence problems. BC107800 mRNA Translation: AAI07801.1 Sequence problems. M73837 mRNA Translation: AAA59870.1 BX537690 mRNA Translation: CAD97814.1 BX641020 mRNA Translation: CAE46013.1 |
CCDSi | CCDS31208.1 [Q14865-1] CCDS58082.1 [Q14865-2] |
PIRi | S27963 |
RefSeqi | NP_001231567.1, NM_001244638.1 [Q14865-2] NP_115575.1, NM_032199.2 [Q14865-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1IG6 | NMR | - | A | 318-424 | [»] | |
2OEH | NMR | - | A | 318-424 | [»] | |
BMRBi | Q14865 | |||||
SMRi | Q14865 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 123918, 50 interactors |
IntActi | Q14865, 25 interactors |
MINTi | Q14865 |
STRINGi | 9606.ENSP00000279873 |
PTM databases
iPTMneti | Q14865 |
PhosphoSitePlusi | Q14865 |
Genetic variation databases
BioMutai | ARID5B |
DMDMi | 209572763 |
Proteomic databases
EPDi | Q14865 |
jPOSTi | Q14865 |
MassIVEi | Q14865 |
MaxQBi | Q14865 |
PaxDbi | Q14865 |
PeptideAtlasi | Q14865 |
PRIDEi | Q14865 |
ProteomicsDBi | 60211 [Q14865-1] 60212 [Q14865-2] 60213 [Q14865-3] |
Protocols and materials databases
Antibodypediai | 2974, 123 antibodies from 24 providers |
DNASUi | 84159 |
Genome annotation databases
Ensembli | ENST00000279873; ENSP00000279873; ENSG00000150347 ENST00000309334; ENSP00000308862; ENSG00000150347 [Q14865-2] |
GeneIDi | 84159 |
KEGGi | hsa:84159 |
MANE-Selecti | ENST00000279873.12; ENSP00000279873.7; NM_032199.3; NP_115575.1 |
UCSCi | uc001jlt.3, human [Q14865-1] |
Organism-specific databases
CTDi | 84159 |
DisGeNETi | 84159 |
GeneCardsi | ARID5B |
HGNCi | HGNC:17362, ARID5B |
HPAi | ENSG00000150347, Tissue enhanced (skeletal) |
MIMi | 608538, gene 613065, phenotype |
neXtProti | NX_Q14865 |
OpenTargetsi | ENSG00000150347 |
PharmGKBi | PA134943193 |
VEuPathDBi | HostDB:ENSG00000150347 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2744, Eukaryota |
GeneTreei | ENSGT00940000161078 |
HOGENOMi | CLU_007985_0_0_1 |
InParanoidi | Q14865 |
OMAi | SCPQRRD |
OrthoDBi | 368297at2759 |
PhylomeDBi | Q14865 |
TreeFami | TF324725 |
Enzyme and pathway databases
PathwayCommonsi | Q14865 |
Reactomei | R-HSA-3214842, HDMs demethylate histones |
SignaLinki | Q14865 |
SIGNORi | Q14865 |
Miscellaneous databases
BioGRID-ORCSi | 84159, 32 hits in 1065 CRISPR screens |
ChiTaRSi | ARID5B, human |
EvolutionaryTracei | Q14865 |
GeneWikii | ARID5B |
GenomeRNAii | 84159 |
Pharosi | Q14865, Tbio |
PROi | PR:Q14865 |
RNActi | Q14865, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000150347, Expressed in uterine cervix and 258 other tissues |
ExpressionAtlasi | Q14865, baseline and differential |
Genevisiblei | Q14865, HS |
Family and domain databases
Gene3Di | 1.10.150.60, 1 hit |
InterProi | View protein in InterPro IPR030408, ARID5B IPR001606, ARID_dom IPR036431, ARID_dom_sf |
PANTHERi | PTHR13964:SF37, PTHR13964:SF37, 1 hit |
Pfami | View protein in Pfam PF01388, ARID, 1 hit |
SMARTi | View protein in SMART SM00501, BRIGHT, 1 hit |
SUPFAMi | SSF46774, SSF46774, 1 hit |
PROSITEi | View protein in PROSITE PS51011, ARID, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ARI5B_HUMAN | |
Accessioni | Q14865Primary (citable) accession number: Q14865 Secondary accession number(s): B4DLB3 Q9H786 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 2, 2004 |
Last sequence update: | October 14, 2008 | |
Last modified: | February 23, 2022 | |
This is version 190 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 10
Human chromosome 10: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families