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UniProtKB - Q14865 (ARI5B_HUMAN)

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Protein

AT-rich interactive domain-containing protein 5B

Gene

ARID5B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription coactivator that binds to the 5'-AATA[CT]-3' core sequence and plays a key role in adipogenesis and liver development. Acts by forming a complex with phosphorylated PHF2, which mediates demethylation at Lys-336, leading to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. Required for adipogenesis: regulates triglyceride metabolism in adipocytes by regulating expression of adipogenic genes. Overexpression leads to induction of smooth muscle marker genes, suggesting that it may also act as a regulator of smooth muscle cell differentiation and proliferation. Represses the cytomegalovirus enhancer.1 Publication

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-3214842 HDMs demethylate histones
SignaLinkiQ14865

Names & Taxonomyi

Protein namesi
Recommended name:
AT-rich interactive domain-containing protein 5B
Short name:
ARID domain-containing protein 5B
Alternative name(s):
MRF1-like protein
Modulator recognition factor 2
Short name:
MRF-2
Gene namesi
Name:ARID5B
Synonyms:DESRT, MRF2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

EuPathDBiHostDB:ENSG00000150347.14
HGNCiHGNC:17362 ARID5B
MIMi608538 gene
neXtProtiNX_Q14865

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Involvement in diseasei

Defects in ARID5B may be a cause of susceptibility to coronary atherosclerosis in the Japanese population.
Leukemia, acute lymphoblastic (ALL)7 Publications
Disease susceptibility is associated with variations affecting the gene represented in this entry.
Disease descriptionA subtype of acute leukemia, a cancer of the white blood cells. ALL is a malignant disease of bone marrow and the most common malignancy diagnosed in children. The malignant cells are lymphoid precursor cells (lymphoblasts) that are arrested in an early stage of development. The lymphoblasts replace the normal marrow elements, resulting in a marked decrease in the production of normal blood cells. Consequently, anemia, thrombocytopenia, and neutropenia occur to varying degrees. The lymphoblasts also proliferate in organs other than the marrow, particularly the liver, spleen, and lymphnodes.
See also OMIM:613065

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi336K → A or R: Abolishes methylation and FSK-dependent DNA-binding of the PHF2-ARID5B complex to promoters. 1 Publication1

Organism-specific databases

DisGeNETi84159
MIMi613065 phenotype
OpenTargetsiENSG00000150347
PharmGKBiPA134943193

Polymorphism and mutation databases

BioMutaiARID5B
DMDMi209572763

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002005811 – 1188AT-rich interactive domain-containing protein 5BAdd BLAST1188

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki130Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei264PhosphoserineCombined sources1
Modified residuei336N6,N6-dimethyllysine1 Publication1
Cross-linki445Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki494Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki496Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki767Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki774Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki803Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki810Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki893Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki916Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki920Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki935Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki988Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki1000Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki1013Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei1032PhosphoserineCombined sources1
Cross-linki1055Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki1070Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei1133PhosphoserineCombined sources1

Post-translational modificationi

Methylation at Lys-336 prevents DNA-binding. Demethylation by PHF2 promotes recruitment of the PHF2-ARID5B complex to promoters.1 Publication

Keywords - PTMi

Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ14865
MaxQBiQ14865
PaxDbiQ14865
PeptideAtlasiQ14865
PRIDEiQ14865
ProteomicsDBi60211
60212 [Q14865-2]
60213 [Q14865-3]

PTM databases

iPTMnetiQ14865
PhosphoSitePlusiQ14865

Expressioni

Tissue specificityi

Widely expressed, including in liver (at protein level).1 Publication

Gene expression databases

BgeeiENSG00000150347
CleanExiHS_ARID5B
GenevisibleiQ14865 HS

Organism-specific databases

HPAiHPA015037

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
HDAC1Q135475EBI-1210388,EBI-301834

Protein-protein interaction databases

BioGridi123918, 21 interactors
IntActiQ14865, 15 interactors
STRINGi9606.ENSP00000279873

Structurei

Secondary structure

11188
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi319 – 333Combined sources15
Turni334 – 336Combined sources3
Helixi339 – 341Combined sources3
Beta strandi345 – 349Combined sources5
Helixi352 – 361Combined sources10
Helixi364 – 371Combined sources8
Helixi374 – 381Combined sources8
Turni388 – 393Combined sources6
Helixi394 – 401Combined sources8
Turni402 – 405Combined sources4
Helixi406 – 412Combined sources7
Beta strandi414 – 417Combined sources4

3D structure databases

ProteinModelPortaliQ14865
SMRiQ14865
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ14865

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini318 – 410ARIDPROSITE-ProRule annotationAdd BLAST93

Domaini

The ARID domain mediates the interaction with DNA.

Sequence similaritiesi

Belongs to the ARID5B family.Curated

Phylogenomic databases

eggNOGiENOG410IQ7B Eukaryota
ENOG4110NWK LUCA
GeneTreeiENSGT00900000140901
HOVERGENiHBG102783
InParanoidiQ14865
OMAiPKACRVS
OrthoDBiEOG091G0E6N
PhylomeDBiQ14865
TreeFamiTF324725

Family and domain databases

Gene3Di1.10.150.60, 1 hit
InterProiView protein in InterPro
IPR030408 ARID5B
IPR001606 ARID_dom
IPR036431 ARID_dom_sf
PANTHERiPTHR13964:SF37 PTHR13964:SF37, 1 hit
PfamiView protein in Pfam
PF01388 ARID, 1 hit
SMARTiView protein in SMART
SM00501 BRIGHT, 1 hit
SUPFAMiSSF46774 SSF46774, 1 hit
PROSITEiView protein in PROSITE
PS51011 ARID, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q14865-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEPNSLQWVG SPCGLHGPYI FYKAFQFHLE GKPRILSLGD FFFVRCTPKD 
        60         70         80         90        100
PICIAELQLL WEERTSRQLL SSSKLYFLPE DTPQGRNSDH GEDEVIAVSE 
       110        120        130        140        150
KVIVKLEDLV KWVHSDFSKW RCGFHAGPVK TEALGRNGQK EALLKYRQST 
       160        170        180        190        200
LNSGLNFKDV LKEKADLGED EEETNVIVLS YPQYCRYRSM LKRIQDKPSS 
       210        220        230        240        250
ILTDQFALAL GGIAVVSRNP QILYCRDTFD HPTLIENESI CDEFAPNLKG 
       260        270        280        290        300
RPRKKKPCPQ RRDSFSGVKD SNNNSDGKAV AKVKCEARSA LTKPKNNHNC 
       310        320        330        340        350
KKVSNEEKPK VAIGEECRAD EQAFLVALYK YMKERKTPIE RIPYLGFKQI 
       360        370        380        390        400
NLWTMFQAAQ KLGGYETITA RRQWKHIYDE LGGNPGSTSA ATCTRRHYER 
       410        420        430        440        450
LILPYERFIK GEEDKPLPPI KPRKQENSSQ ENENKTKVSG TKRIKHEIPK 
       460        470        480        490        500
SKKEKENAPK PQDAAEVSSE QEKEQETLIS QKSIPEPLPA ADMKKKIEGY 
       510        520        530        540        550
QEFSAKPLAS RVDPEKDNET DQGSNSEKVA EEAGEKGPTP PLPSAPLAPE 
       560        570        580        590        600
KDSALVPGAS KQPLTSPSAL VDSKQESKLC CFTESPESEP QEASFPSFPT 
       610        620        630        640        650
TQPPLANQNE TEDDKLPAMA DYIANCTVKV DQLGSDDIHN ALKQTPKVLV 
       660        670        680        690        700
VQSFDMFKDK DLTGPMNENH GLNYTPLLYS RGNPGIMSPL AKKKLLSQVS 
       710        720        730        740        750
GASLSSSYPY GSPPPLISKK KLIARDDLCS SLSQTHHGQS TDHMAVSRPS 
       760        770        780        790        800
VIQHVQSFRS KPSEERKTIN DIFKHEKLSR SDPHRCSFSK HHLNPLADSY 
       810        820        830        840        850
VLKQEIQEGK DKLLEKRALP HSHMPSFLAD FYSSPHLHSL YRHTEHHLHN 
       860        870        880        890        900
EQTSKYPSRD MYRESENSSF PSHRHQEKLH VNYLTSLHLQ DKKSAAAEAP 
       910        920        930        940        950
TDDQPTDLSL PKNPHKPTGK VLGLAHSTTG PQESKGISQF QVLGSQSRDC 
       960        970        980        990       1000
HPKACRVSPM TMSGPKKYPE SLSRSGKPHH VRLENFRKME GMVHPILHRK 
      1010       1020       1030       1040       1050
MSPQNIGAAR PIKRSLEDLD LVIAGKKARA VSPLDPSKEV SGKEKASEQE 
      1060       1070       1080       1090       1100
SEGSKAAHGG HSGGGSEGHK LPLSSPIFPG LYSGSLCNSG LNSRLPAGYS 
      1110       1120       1130       1140       1150
HSLQYLKNQT VLSPLMQPLA FHSLVMQRGI FTSPTNSQQL YRHLAAATPV 
      1160       1170       1180 
GSSYGDLLHN SIYPLAAINP QAAFPSSQLS SVHPSTKL
Length:1,188
Mass (Da):132,375
Last modified:October 14, 2008 - v3
Checksum:i23B7C525B0055330
GO
Isoform 2 (identifier: Q14865-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-243: Missing.
     244-244: F → M

Show »
Length:945
Mass (Da):104,602
Checksum:iEAD2A25EDB4EF57D
GO
Isoform 3 (identifier: Q14865-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     284-318: KCEARSALTKPKNNHNCKKVSNEEKPKVAIGEECR → RSFTPRYSFRCIFLFLLFLFISLCLCLGGSFQFSI
     319-1188: Missing.

Note: No experimental confirmation available.
Show »
Length:318
Mass (Da):36,282
Checksum:iAFE2D2C2665FE375
GO

Sequence cautioni

The sequence AAH15120 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAH36831 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAH66345 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAI07801 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence BAB15012 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti401L → S in AAH15120 (PubMed:15489334).Curated1
Sequence conflicti451S → N in AAH15120 (PubMed:15489334).Curated1
Sequence conflicti827F → L in CAE46013 (PubMed:17974005).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0093551 – 243Missing in isoform 2. 2 PublicationsAdd BLAST243
Alternative sequenceiVSP_009356244F → M in isoform 2. 2 Publications1
Alternative sequenceiVSP_041560284 – 318KCEAR…GEECR → RSFTPRYSFRCIFLFLLFLF ISLCLCLGGSFQFSI in isoform 3. CuratedAdd BLAST35
Alternative sequenceiVSP_041561319 – 1188Missing in isoform 3. CuratedAdd BLAST870

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC067742 Genomic DNA No translation available.
AL671972 Genomic DNA No translation available.
AK024803 mRNA Translation: BAB15012.1 Sequence problems.
AK296921 mRNA Translation: BAG59475.1
BC015120 mRNA Translation: AAH15120.1 Sequence problems.
BC036831 mRNA Translation: AAH36831.1 Different initiation.
BC066345 mRNA Translation: AAH66345.1 Sequence problems.
BC107800 mRNA Translation: AAI07801.1 Sequence problems.
M73837 mRNA Translation: AAA59870.1
BX537690 mRNA Translation: CAD97814.1
BX641020 mRNA Translation: CAE46013.1
CCDSiCCDS31208.1 [Q14865-1]
CCDS58082.1 [Q14865-2]
PIRiS27963
RefSeqiNP_001231567.1, NM_001244638.1 [Q14865-2]
NP_115575.1, NM_032199.2 [Q14865-1]
UniGeneiHs.535297

Genome annotation databases

EnsembliENST00000279873; ENSP00000279873; ENSG00000150347 [Q14865-1]
ENST00000309334; ENSP00000308862; ENSG00000150347 [Q14865-2]
GeneIDi84159
KEGGihsa:84159
UCSCiuc001jlt.3 human [Q14865-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiARI5B_HUMAN
AccessioniPrimary (citable) accession number: Q14865
Secondary accession number(s): B4DLB3
, Q05DG6, Q32Q59, Q5VST4, Q6NZ42, Q7Z3M4, Q8N421, Q9H786
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: October 14, 2008
Last modified: July 18, 2018
This is version 168 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

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