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UniProtKB - Q14863 (PO6F1_HUMAN)
Protein
POU domain, class 6, transcription factor 1
Gene
POU6F1
Organism
Homo sapiens (Human)
Status
Functioni
Transcription factor that binds preferentially to a variant of the octamer motif (5'-ATGATAAT-3').
By similarityRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 234 – 293 | HomeoboxPROSITE-ProRule annotationAdd BLAST | 60 |
GO - Molecular functioni
- DNA-binding transcription factor activity Source: ProtInc
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: NTNU_SB
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: NTNU_SB
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- sequence-specific DNA binding Source: NTNU_SB
- sequence-specific double-stranded DNA binding Source: ARUK-UCL
GO - Biological processi
- brain development Source: ProtInc
- heart development Source: ProtInc
- muscle organ development Source: ProtInc
- negative regulation of transcription by RNA polymerase II Source: NTNU_SB
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | DNA-binding |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | Q14863 |
SignaLinki | Q14863 |
Names & Taxonomyi
Protein namesi | Recommended name: POU domain, class 6, transcription factor 1Alternative name(s): Brain-specific homeobox/POU domain protein 5 Short name: Brain-5 Short name: Brn-5 mPOU homeobox protein |
Gene namesi | Name:POU6F1 Synonyms:BRN5, MPOU, TCFB1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:9224, POU6F1 |
MIMi | 618043, gene |
neXtProti | NX_Q14863 |
Pathology & Biotechi
Organism-specific databases
DisGeNETi | 5463 |
PharmGKBi | PA33548 |
Miscellaneous databases
Pharosi | Q14863, Tbio |
Genetic variation databases
BioMutai | POU6F1 |
DMDMi | 2495306 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000100759 | 1 – 301 | POU domain, class 6, transcription factor 1Add BLAST | 301 |
Proteomic databases
MassIVEi | Q14863 |
PaxDbi | Q14863 |
PeptideAtlasi | Q14863 |
PRIDEi | Q14863 |
PTM databases
iPTMneti | Q14863 |
PhosphoSitePlusi | Q14863 |
Expressioni
Tissue specificityi
In the embryo, expressed exclusively in the developing brain, whereas in the adult its expression is restricted to brain, heart, skeletal muscle and lung. In the brain, the highest expression levels are found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum.
Gene expression databases
Bgeei | ENSG00000184271, Expressed in forebrain and 237 other tissues |
Genevisiblei | Q14863, HS |
Interactioni
Binary interactionsi
Q14863
With | #Exp. | IntAct |
---|---|---|
ACOT7 - isoform 4 [O00154-4] | 3 | EBI-18138148,EBI-12007918 |
NFYC - isoform 1 [Q13952-2] | 3 | EBI-18138148,EBI-11956831 |
Protein-protein interaction databases
BioGRIDi | 111459, 3 interactors |
IntActi | Q14863, 3 interactors |
STRINGi | 9606.ENSP00000373895 |
Miscellaneous databases
RNActi | Q14863, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q14863 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 139 – 213 | POU-specificPROSITE-ProRule annotationAdd BLAST | 75 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 62 – 93 | DisorderedSequence analysisAdd BLAST | 32 |
Sequence similaritiesi
Keywords - Domaini
HomeoboxPhylogenomic databases
eggNOGi | KOG3802, Eukaryota |
HOGENOMi | CLU_013065_6_0_1 |
InParanoidi | Q14863 |
OMAi | SEMQPIQ |
OrthoDBi | 474850at2759 |
PhylomeDBi | Q14863 |
TreeFami | TF350705 |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
Gene3Di | 1.10.260.40, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR001356, Homeobox_dom IPR010982, Lambda_DNA-bd_dom_sf IPR013847, POU IPR000327, POU_dom |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit PF00157, Pou, 1 hit |
PRINTSi | PR00028, POUDOMAIN |
SMARTi | View protein in SMART SM00389, HOX, 1 hit SM00352, POU, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit SSF47413, SSF47413, 1 hit |
PROSITEi | View protein in PROSITE PS50071, HOMEOBOX_2, 1 hit PS00035, POU_1, 1 hit PS00465, POU_2, 1 hit PS51179, POU_3, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
Q14863-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPGISSQILT NAQGQVIGTL PWVVNSASVA APAPAQSLQV QAVTPQLLLN
60 70 80 90 100
AQGQVIATLA SSPLPPPVAV RKPSTPESPA KSEVQPIQPT PTVPQPAVVI
110 120 130 140 150
ASPAPAAKPS ASAPIPITCS ETPTVSQLVS KPHTPSLDED GINLEEIREF
160 170 180 190 200
AKNFKIRRLS LGLTQTQVGQ ALTATEGPAY SQSAICRFEK LDITPKSAQK
210 220 230 240 250
LKPVLEKWLN EAELRNQEGQ QNLMEFVGGE PSKKRKRRTS FTPQAIEALN
260 270 280 290 300
AYFEKNPLPT GQEITEIAKE LNYDREVVRV WFCNRRQTLK NTSKLNVFQI
P
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A1C7CYV8 | A0A1C7CYV8_HUMAN | POU domain protein | POU6F1 | 611 | Annotation score: | ||
A0A1C7CYY7 | A0A1C7CYY7_HUMAN | POU domain protein | POU6F1 | 577 | Annotation score: | ||
A0A1B0GU61 | A0A1B0GU61_HUMAN | POU domain, class 6, transcription ... | POU6F1 | 248 | Annotation score: | ||
A0A1B0GVK9 | A0A1B0GVK9_HUMAN | POU domain, class 6, transcription ... | POU6F1 | 180 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 210 | N → M in CAA79978 (PubMed:7908264).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z21966 mRNA Translation: CAA79977.1 Z21967 mRNA Translation: CAA79978.1 L14482 mRNA No translation available. CH471111 Genomic DNA Translation: EAW58180.1 BC051326 mRNA Translation: AAH51326.2 BC074765 mRNA Translation: AAH74765.3 |
PIRi | A54687 |
RefSeqi | NP_001317351.1, NM_001330422.1 NP_002693.3, NM_002702.3 XP_016875013.1, XM_017019524.1 XP_016875014.1, XM_017019525.1 XP_016875015.1, XM_017019526.1 XP_016875016.1, XM_017019527.1 XP_016875017.1, XM_017019528.1 XP_016875018.1, XM_017019529.1 XP_016875019.1, XM_017019530.1 |
Genome annotation databases
GeneIDi | 5463 |
KEGGi | hsa:5463 |
UCSCi | uc001rxy.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z21966 mRNA Translation: CAA79977.1 Z21967 mRNA Translation: CAA79978.1 L14482 mRNA No translation available. CH471111 Genomic DNA Translation: EAW58180.1 BC051326 mRNA Translation: AAH51326.2 BC074765 mRNA Translation: AAH74765.3 |
PIRi | A54687 |
RefSeqi | NP_001317351.1, NM_001330422.1 NP_002693.3, NM_002702.3 XP_016875013.1, XM_017019524.1 XP_016875014.1, XM_017019525.1 XP_016875015.1, XM_017019526.1 XP_016875016.1, XM_017019527.1 XP_016875017.1, XM_017019528.1 XP_016875018.1, XM_017019529.1 XP_016875019.1, XM_017019530.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3D1N | X-ray | 2.51 | I/J/K/L/M/N/O/P | 142-292 | [»] | |
SMRi | Q14863 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 111459, 3 interactors |
IntActi | Q14863, 3 interactors |
STRINGi | 9606.ENSP00000373895 |
PTM databases
iPTMneti | Q14863 |
PhosphoSitePlusi | Q14863 |
Genetic variation databases
BioMutai | POU6F1 |
DMDMi | 2495306 |
Proteomic databases
MassIVEi | Q14863 |
PaxDbi | Q14863 |
PeptideAtlasi | Q14863 |
PRIDEi | Q14863 |
Protocols and materials databases
DNASUi | 5463 |
Genome annotation databases
GeneIDi | 5463 |
KEGGi | hsa:5463 |
UCSCi | uc001rxy.4, human |
Organism-specific databases
CTDi | 5463 |
DisGeNETi | 5463 |
GeneCardsi | POU6F1 |
HGNCi | HGNC:9224, POU6F1 |
MIMi | 618043, gene |
neXtProti | NX_Q14863 |
PharmGKBi | PA33548 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3802, Eukaryota |
HOGENOMi | CLU_013065_6_0_1 |
InParanoidi | Q14863 |
OMAi | SEMQPIQ |
OrthoDBi | 474850at2759 |
PhylomeDBi | Q14863 |
TreeFami | TF350705 |
Enzyme and pathway databases
PathwayCommonsi | Q14863 |
SignaLinki | Q14863 |
Miscellaneous databases
BioGRID-ORCSi | 5463, 4 hits in 1061 CRISPR screens |
ChiTaRSi | POU6F1, human |
GenomeRNAii | 5463 |
Pharosi | Q14863, Tbio |
PROi | PR:Q14863 |
RNActi | Q14863, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000184271, Expressed in forebrain and 237 other tissues |
Genevisiblei | Q14863, HS |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
Gene3Di | 1.10.260.40, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR001356, Homeobox_dom IPR010982, Lambda_DNA-bd_dom_sf IPR013847, POU IPR000327, POU_dom |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit PF00157, Pou, 1 hit |
PRINTSi | PR00028, POUDOMAIN |
SMARTi | View protein in SMART SM00389, HOX, 1 hit SM00352, POU, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit SSF47413, SSF47413, 1 hit |
PROSITEi | View protein in PROSITE PS50071, HOMEOBOX_2, 1 hit PS00035, POU_1, 1 hit PS00465, POU_2, 1 hit PS51179, POU_3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PO6F1_HUMAN | |
Accessioni | Q14863Primary (citable) accession number: Q14863 Secondary accession number(s): Q15944, Q6DK47, Q7Z7P6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 188 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families