Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

LIM and SH3 domain protein 1

Gene

LASP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays an important role in the regulation of dynamic actin-based, cytoskeletal activities. Agonist-dependent changes in LASP1 phosphorylation may also serve to regulate actin-associated ion transport activities, not only in the parietal cell but also in certain other F-actin-rich secretory epithelial cell types (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActin-binding
Biological processIon transport, Transport
LigandMetal-binding, Zinc

Enzyme and pathway databases

SIGNORiQ14847

Names & Taxonomyi

Protein namesi
Recommended name:
LIM and SH3 domain protein 1
Short name:
LASP-1
Alternative name(s):
Metastatic lymph node gene 50 protein
Short name:
MLN 50
Gene namesi
Name:LASP1
Synonyms:MLN50
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000002834.17
HGNCiHGNC:6513 LASP1
MIMi602920 gene
neXtProtiNX_Q14847

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Organism-specific databases

DisGeNETi3927
OpenTargetsiENSG00000002834
PharmGKBiPA30298

Polymorphism and mutation databases

DMDMi3122342

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000757611 – 261LIM and SH3 domain protein 1Add BLAST261

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine1 Publication1
Modified residuei42N6-acetyllysineCombined sources1
Modified residuei68PhosphothreonineCombined sources1
Modified residuei75N6-methyllysineCombined sources1
Modified residuei99PhosphoserineCombined sources1
Modified residuei104PhosphothreonineCombined sources1
Modified residuei112N6-succinyllysineBy similarity1
Modified residuei118PhosphoserineCombined sources1
Modified residuei134PhosphoserineCombined sources1
Modified residuei146PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiQ14847
MaxQBiQ14847
PaxDbiQ14847
PeptideAtlasiQ14847
PRIDEiQ14847
ProteomicsDBi60206
60207 [Q14847-2]

2D gel databases

OGPiQ14847
SWISS-2DPAGEiQ14847

PTM databases

iPTMnetiQ14847
PhosphoSitePlusiQ14847
SwissPalmiQ14847

Expressioni

Gene expression databases

BgeeiENSG00000002834 Expressed in 238 organ(s), highest expression level in upper lobe of lung
CleanExiHS_LASP1
ExpressionAtlasiQ14847 baseline and differential
GenevisibleiQ14847 HS

Organism-specific databases

HPAiCAB022049
HPA012072

Interactioni

Subunit structurei

Interacts with F-actin (By similarity). Interacts with ANKRD54 (By similarity). Interacts with KBTBD10.By similarity1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi110120, 90 interactors
IntActiQ14847, 124 interactors
MINTiQ14847
STRINGi9606.ENSP00000325240

Structurei

Secondary structure

1261
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ14847
SMRiQ14847
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ14847

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 56LIM zinc-bindingPROSITE-ProRule annotationAdd BLAST52
Repeati61 – 95Nebulin 1Add BLAST35
Repeati97 – 131Nebulin 2Add BLAST35
Domaini202 – 261SH3PROSITE-ProRule annotationAdd BLAST60

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi201 – 204Poly-Gly4

Keywords - Domaini

LIM domain, Repeat, SH3 domain

Phylogenomic databases

eggNOGiKOG1702 Eukaryota
ENOG4111GQ8 LUCA
GeneTreeiENSGT00530000062924
HOGENOMiHOG000006616
HOVERGENiHBG054636
InParanoidiQ14847
OMAiYGYKEPA
OrthoDBiEOG091G0U2O
PhylomeDBiQ14847
TreeFamiTF319104

Family and domain databases

CDDicd11934 SH3_Lasp1_C, 1 hit
InterProiView protein in InterPro
IPR035630 Lasp1_SH3
IPR000900 Nebulin_repeat
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
IPR001781 Znf_LIM
PfamiView protein in Pfam
PF00412 LIM, 1 hit
PF00880 Nebulin, 2 hits
PF14604 SH3_9, 1 hit
PRINTSiPR00452 SH3DOMAIN
SMARTiView protein in SMART
SM00132 LIM, 1 hit
SM00227 NEBU, 2 hits
SM00326 SH3, 1 hit
SUPFAMiSSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS00478 LIM_DOMAIN_1, 1 hit
PS50023 LIM_DOMAIN_2, 1 hit
PS51216 NEBULIN, 2 hits
PS50002 SH3, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q14847-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MNPNCARCGK IVYPTEKVNC LDKFWHKACF HCETCKMTLN MKNYKGYEKK
60 70 80 90 100
PYCNAHYPKQ SFTMVADTPE NLRLKQQSEL QSQVRYKEEF EKNKGKGFSV
110 120 130 140 150
VADTPELQRI KKTQDQISNI KYHEEFEKSR MGPSGGEGME PERRDSQDGS
160 170 180 190 200
SYRRPLEQQQ PHHIPTSAPV YQQPQQQPVA QSYGGYKEPA APVSIQRSAP
210 220 230 240 250
GGGGKRYRAV YDYSAADEDE VSFQDGDTIV NVQQIDDGWM YGTVERTGDT
260
GMLPANYVEA I
Length:261
Mass (Da):29,717
Last modified:January 1, 1998 - v2
Checksum:i3B89B988605B3639
GO
Isoform 2 (identifier: Q14847-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     201-261: GGGGKRYRAV...MLPANYVEAI → ICLQHIPRHR...LTYPHIPGLG

Note: No experimental confirmation available.
Show »
Length:323
Mass (Da):36,014
Checksum:iB6A28D1D97AB2C69
GO
Isoform 3 (identifier: Q14847-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-83: MNPNCARCGK...LKQQSELQSQ → MLPLRDLQDDTEHEELQGLREEALLQR

Note: No experimental confirmation available.
Show »
Length:205
Mass (Da):23,181
Checksum:i4CD6D2622F74CCF4
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F6S2S5F6S2S5_HUMAN
LIM and SH3 domain protein 1
LASP1
91Annotation score:
K7ESD6K7ESD6_HUMAN
LIM and SH3 domain protein 1
LASP1
71Annotation score:
C9J9W2C9J9W2_HUMAN
LIM and SH3 domain protein 1
LASP1
166Annotation score:
J3KSN1J3KSN1_HUMAN
LIM and SH3 domain protein 1
LASP1
105Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti79E → R in AAH12460 (PubMed:15489334).Curated1
Sequence conflicti210V → A in AAH12460 (PubMed:15489334).Curated1
Sequence conflicti220E → A in AAH12460 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0546111 – 83MNPNC…ELQSQ → MLPLRDLQDDTEHEELQGLR EEALLQR in isoform 3. 1 PublicationAdd BLAST83
Alternative sequenceiVSP_016554201 – 261GGGGK…YVEAI → ICLQHIPRHRIRPGRDPSIL QCLCFLKPATACDSYPSSSF FCQLKPSSATSAGSLLWQAS PLIDFLVFSLDGTGMGLSGG GRGPWGRAGMGDLLACGPHL PLCSLPSHPPAQLLTYPHIP GLG in isoform 2. 1 PublicationAdd BLAST61

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82456 mRNA Translation: CAA57833.1
AK294704 mRNA Translation: BAG57861.1
AC006441 Genomic DNA No translation available.
AC110749 Genomic DNA No translation available.
BC007560 mRNA Translation: AAH07560.1
BC012460 mRNA Translation: AAH12460.1
CCDSiCCDS11331.1 [Q14847-1]
CCDS62164.1 [Q14847-3]
PIRiS68234
RefSeqiNP_001258537.1, NM_001271608.1 [Q14847-3]
NP_006139.1, NM_006148.3 [Q14847-1]
UniGeneiHs.741156

Genome annotation databases

EnsembliENST00000318008; ENSP00000325240; ENSG00000002834 [Q14847-1]
ENST00000433206; ENSP00000401048; ENSG00000002834 [Q14847-3]
ENST00000435347; ENSP00000392853; ENSG00000002834 [Q14847-1]
GeneIDi3927
KEGGihsa:3927
UCSCiuc002hra.3 human [Q14847-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82456 mRNA Translation: CAA57833.1
AK294704 mRNA Translation: BAG57861.1
AC006441 Genomic DNA No translation available.
AC110749 Genomic DNA No translation available.
BC007560 mRNA Translation: AAH07560.1
BC012460 mRNA Translation: AAH12460.1
CCDSiCCDS11331.1 [Q14847-1]
CCDS62164.1 [Q14847-3]
PIRiS68234
RefSeqiNP_001258537.1, NM_001271608.1 [Q14847-3]
NP_006139.1, NM_006148.3 [Q14847-1]
UniGeneiHs.741156

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3I35X-ray1.40A202-261[»]
ProteinModelPortaliQ14847
SMRiQ14847
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110120, 90 interactors
IntActiQ14847, 124 interactors
MINTiQ14847
STRINGi9606.ENSP00000325240

PTM databases

iPTMnetiQ14847
PhosphoSitePlusiQ14847
SwissPalmiQ14847

Polymorphism and mutation databases

DMDMi3122342

2D gel databases

OGPiQ14847
SWISS-2DPAGEiQ14847

Proteomic databases

EPDiQ14847
MaxQBiQ14847
PaxDbiQ14847
PeptideAtlasiQ14847
PRIDEiQ14847
ProteomicsDBi60206
60207 [Q14847-2]

Protocols and materials databases

DNASUi3927
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000318008; ENSP00000325240; ENSG00000002834 [Q14847-1]
ENST00000433206; ENSP00000401048; ENSG00000002834 [Q14847-3]
ENST00000435347; ENSP00000392853; ENSG00000002834 [Q14847-1]
GeneIDi3927
KEGGihsa:3927
UCSCiuc002hra.3 human [Q14847-1]

Organism-specific databases

CTDi3927
DisGeNETi3927
EuPathDBiHostDB:ENSG00000002834.17
GeneCardsiLASP1
HGNCiHGNC:6513 LASP1
HPAiCAB022049
HPA012072
MIMi602920 gene
neXtProtiNX_Q14847
OpenTargetsiENSG00000002834
PharmGKBiPA30298
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1702 Eukaryota
ENOG4111GQ8 LUCA
GeneTreeiENSGT00530000062924
HOGENOMiHOG000006616
HOVERGENiHBG054636
InParanoidiQ14847
OMAiYGYKEPA
OrthoDBiEOG091G0U2O
PhylomeDBiQ14847
TreeFamiTF319104

Enzyme and pathway databases

SIGNORiQ14847

Miscellaneous databases

ChiTaRSiLASP1 human
EvolutionaryTraceiQ14847
GeneWikiiLASP1
GenomeRNAii3927
PROiPR:Q14847
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000002834 Expressed in 238 organ(s), highest expression level in upper lobe of lung
CleanExiHS_LASP1
ExpressionAtlasiQ14847 baseline and differential
GenevisibleiQ14847 HS

Family and domain databases

CDDicd11934 SH3_Lasp1_C, 1 hit
InterProiView protein in InterPro
IPR035630 Lasp1_SH3
IPR000900 Nebulin_repeat
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
IPR001781 Znf_LIM
PfamiView protein in Pfam
PF00412 LIM, 1 hit
PF00880 Nebulin, 2 hits
PF14604 SH3_9, 1 hit
PRINTSiPR00452 SH3DOMAIN
SMARTiView protein in SMART
SM00132 LIM, 1 hit
SM00227 NEBU, 2 hits
SM00326 SH3, 1 hit
SUPFAMiSSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS00478 LIM_DOMAIN_1, 1 hit
PS50023 LIM_DOMAIN_2, 1 hit
PS51216 NEBULIN, 2 hits
PS50002 SH3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiLASP1_HUMAN
AccessioniPrimary (citable) accession number: Q14847
Secondary accession number(s): B4DGQ0, Q96ED2, Q96IG0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: September 12, 2018
This is version 182 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  2. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again