UniProtKB - Q14847 (LASP1_HUMAN)
LIM and SH3 domain protein 1
LASP1
Functioni
Plays an important role in the regulation of dynamic actin-based, cytoskeletal activities. Agonist-dependent changes in LASP1 phosphorylation may also serve to regulate actin-associated ion transport activities, not only in the parietal cell but also in certain other F-actin-rich secretory epithelial cell types (By similarity).
By similarityGO - Molecular functioni
- actin filament binding Source: GO_Central
- cadherin binding Source: BHF-UCL
- ion transmembrane transporter activity Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- ion transport Source: UniProtKB
Keywordsi
Molecular function | Actin-binding |
Biological process | Ion transport, Transport |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q14847 |
SignaLinki | Q14847 |
SIGNORi | Q14847 |
Names & Taxonomyi
Protein namesi | Recommended name: LIM and SH3 domain protein 1Short name: LASP-1 Alternative name(s): Metastatic lymph node gene 50 protein Short name: MLN 50 |
Gene namesi | Name:LASP1 Synonyms:MLN50 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:6513, LASP1 |
MIMi | 602920, gene |
neXtProti | NX_Q14847 |
VEuPathDBi | HostDB:ENSG00000002834 |
Subcellular locationi
Cytoskeleton
- cytoskeleton By similarity
Other locations
- cell cortex By similarity
Note: Associated with the F-actin rich cortical cytoskeleton.By similarity
Cytoskeleton
- cortical actin cytoskeleton Source: UniProtKB
Other locations
- cytoplasm Source: BHF-UCL
- focal adhesion Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, CytoskeletonPathology & Biotechi
Organism-specific databases
DisGeNETi | 3927 |
OpenTargetsi | ENSG00000002834 |
PharmGKBi | PA30298 |
Miscellaneous databases
Pharosi | Q14847, Tbio |
Genetic variation databases
BioMutai | LASP1 |
DMDMi | 3122342 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000075761 | 1 – 261 | LIM and SH3 domain protein 1Add BLAST | 261 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionine1 Publication | 1 | |
Modified residuei | 42 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 68 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 75 | N6-methyllysineCombined sources | 1 | |
Modified residuei | 99 | PhosphoserineCombined sources | 1 | |
Modified residuei | 104 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 112 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 118 | PhosphoserineCombined sources | 1 | |
Modified residuei | 134 | PhosphoserineCombined sources | 1 | |
Modified residuei | 146 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, Methylation, PhosphoproteinProteomic databases
CPTACi | CPTAC-86 CPTAC-87 CPTAC-928 |
EPDi | Q14847 |
jPOSTi | Q14847 |
MassIVEi | Q14847 |
MaxQBi | Q14847 |
PaxDbi | Q14847 |
PeptideAtlasi | Q14847 |
PRIDEi | Q14847 |
ProteomicsDBi | 4149 60206 [Q14847-1] 60207 [Q14847-2] |
2D gel databases
OGPi | Q14847 |
SWISS-2DPAGEi | Q14847 |
PTM databases
GlyGeni | Q14847, 4 sites, 2 O-linked glycans (4 sites) |
iPTMneti | Q14847 |
MetOSitei | Q14847 |
PhosphoSitePlusi | Q14847 |
SwissPalmi | Q14847 |
Expressioni
Gene expression databases
Bgeei | ENSG00000002834, Expressed in upper lobe of lung and 251 other tissues |
ExpressionAtlasi | Q14847, baseline and differential |
Genevisiblei | Q14847, HS |
Organism-specific databases
HPAi | ENSG00000002834, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with F-actin (By similarity).
Interacts with ANKRD54 (By similarity).
Interacts with KBTBD10.
By similarity1 PublicationBinary interactionsi
Q14847
Isoform 2 [Q14847-2]
GO - Molecular functioni
- actin filament binding Source: GO_Central
- cadherin binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 110120, 205 interactors |
IntActi | Q14847, 165 interactors |
MINTi | Q14847 |
STRINGi | 9606.ENSP00000325240 |
Miscellaneous databases
RNActi | Q14847, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q14847 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q14847 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 5 – 56 | LIM zinc-bindingPROSITE-ProRule annotationAdd BLAST | 52 | |
Repeati | 61 – 95 | Nebulin 1Add BLAST | 35 | |
Repeati | 97 – 131 | Nebulin 2Add BLAST | 35 | |
Domaini | 202 – 261 | SH3PROSITE-ProRule annotationAdd BLAST | 60 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 111 – 186 | DisorderedSequence analysisAdd BLAST | 76 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 116 – 151 | Basic and acidic residuesSequence analysisAdd BLAST | 36 | |
Compositional biasi | 152 – 182 | Polar residuesSequence analysisAdd BLAST | 31 |
Keywords - Domaini
LIM domain, Repeat, SH3 domainPhylogenomic databases
eggNOGi | KOG1702, Eukaryota |
GeneTreei | ENSGT00940000154775 |
HOGENOMi | CLU_026811_0_1_1 |
InParanoidi | Q14847 |
OMAi | QESANYR |
PhylomeDBi | Q14847 |
TreeFami | TF319104 |
Family and domain databases
CDDi | cd11934, SH3_Lasp1_C, 1 hit |
InterProi | View protein in InterPro IPR035630, Lasp1_SH3 IPR000900, Nebulin_repeat IPR036028, SH3-like_dom_sf IPR001452, SH3_domain IPR001781, Znf_LIM |
Pfami | View protein in Pfam PF00412, LIM, 1 hit PF00880, Nebulin, 2 hits PF14604, SH3_9, 1 hit |
PRINTSi | PR00452, SH3DOMAIN |
SMARTi | View protein in SMART SM00132, LIM, 1 hit SM00227, NEBU, 2 hits SM00326, SH3, 1 hit |
SUPFAMi | SSF50044, SSF50044, 1 hit |
PROSITEi | View protein in PROSITE PS00478, LIM_DOMAIN_1, 1 hit PS50023, LIM_DOMAIN_2, 1 hit PS51216, NEBULIN, 2 hits PS50002, SH3, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MNPNCARCGK IVYPTEKVNC LDKFWHKACF HCETCKMTLN MKNYKGYEKK
60 70 80 90 100
PYCNAHYPKQ SFTMVADTPE NLRLKQQSEL QSQVRYKEEF EKNKGKGFSV
110 120 130 140 150
VADTPELQRI KKTQDQISNI KYHEEFEKSR MGPSGGEGME PERRDSQDGS
160 170 180 190 200
SYRRPLEQQQ PHHIPTSAPV YQQPQQQPVA QSYGGYKEPA APVSIQRSAP
210 220 230 240 250
GGGGKRYRAV YDYSAADEDE VSFQDGDTIV NVQQIDDGWM YGTVERTGDT
260
GMLPANYVEA I
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC9J9W2 | C9J9W2_HUMAN | LIM and SH3 domain protein 1 | LASP1 | 166 | Annotation score: | ||
J3KSN1 | J3KSN1_HUMAN | LIM and SH3 domain protein 1 | LASP1 | 105 | Annotation score: | ||
F6S2S5 | F6S2S5_HUMAN | LIM and SH3 domain protein 1 | LASP1 | 91 | Annotation score: | ||
K7ESD6 | K7ESD6_HUMAN | LIM and SH3 domain protein 1 | LASP1 | 71 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 79 | E → R in AAH12460 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 210 | V → A in AAH12460 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 220 | E → A in AAH12460 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_054611 | 1 – 83 | MNPNC…ELQSQ → MLPLRDLQDDTEHEELQGLR EEALLQR in isoform 3. 1 PublicationAdd BLAST | 83 | |
Alternative sequenceiVSP_016554 | 201 – 261 | GGGGK…YVEAI → ICLQHIPRHRIRPGRDPSIL QCLCFLKPATACDSYPSSSF FCQLKPSSATSAGSLLWQAS PLIDFLVFSLDGTGMGLSGG GRGPWGRAGMGDLLACGPHL PLCSLPSHPPAQLLTYPHIP GLG in isoform 2. 1 PublicationAdd BLAST | 61 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X82456 mRNA Translation: CAA57833.1 AK294704 mRNA Translation: BAG57861.1 AC006441 Genomic DNA No translation available. AC110749 Genomic DNA No translation available. BC007560 mRNA Translation: AAH07560.1 BC012460 mRNA Translation: AAH12460.1 |
CCDSi | CCDS11331.1 [Q14847-1] CCDS62164.1 [Q14847-3] |
PIRi | S68234 |
RefSeqi | NP_001258537.1, NM_001271608.1 [Q14847-3] NP_006139.1, NM_006148.3 [Q14847-1] |
Genome annotation databases
Ensembli | ENST00000318008; ENSP00000325240; ENSG00000002834 ENST00000433206; ENSP00000401048; ENSG00000002834 [Q14847-3] ENST00000435347; ENSP00000392853; ENSG00000002834 |
GeneIDi | 3927 |
KEGGi | hsa:3927 |
MANE-Selecti | ENST00000318008.11; ENSP00000325240.6; NM_006148.4; NP_006139.1 |
UCSCi | uc002hra.3, human [Q14847-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X82456 mRNA Translation: CAA57833.1 AK294704 mRNA Translation: BAG57861.1 AC006441 Genomic DNA No translation available. AC110749 Genomic DNA No translation available. BC007560 mRNA Translation: AAH07560.1 BC012460 mRNA Translation: AAH12460.1 |
CCDSi | CCDS11331.1 [Q14847-1] CCDS62164.1 [Q14847-3] |
PIRi | S68234 |
RefSeqi | NP_001258537.1, NM_001271608.1 [Q14847-3] NP_006139.1, NM_006148.3 [Q14847-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3I35 | X-ray | 1.40 | A | 202-261 | [»] | |
SMRi | Q14847 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 110120, 205 interactors |
IntActi | Q14847, 165 interactors |
MINTi | Q14847 |
STRINGi | 9606.ENSP00000325240 |
PTM databases
GlyGeni | Q14847, 4 sites, 2 O-linked glycans (4 sites) |
iPTMneti | Q14847 |
MetOSitei | Q14847 |
PhosphoSitePlusi | Q14847 |
SwissPalmi | Q14847 |
Genetic variation databases
BioMutai | LASP1 |
DMDMi | 3122342 |
2D gel databases
OGPi | Q14847 |
SWISS-2DPAGEi | Q14847 |
Proteomic databases
CPTACi | CPTAC-86 CPTAC-87 CPTAC-928 |
EPDi | Q14847 |
jPOSTi | Q14847 |
MassIVEi | Q14847 |
MaxQBi | Q14847 |
PaxDbi | Q14847 |
PeptideAtlasi | Q14847 |
PRIDEi | Q14847 |
ProteomicsDBi | 4149 60206 [Q14847-1] 60207 [Q14847-2] |
Protocols and materials databases
ABCDi | Q14847, 6 sequenced antibodies |
Antibodypediai | 1894, 403 antibodies from 38 providers |
DNASUi | 3927 |
Genome annotation databases
Ensembli | ENST00000318008; ENSP00000325240; ENSG00000002834 ENST00000433206; ENSP00000401048; ENSG00000002834 [Q14847-3] ENST00000435347; ENSP00000392853; ENSG00000002834 |
GeneIDi | 3927 |
KEGGi | hsa:3927 |
MANE-Selecti | ENST00000318008.11; ENSP00000325240.6; NM_006148.4; NP_006139.1 |
UCSCi | uc002hra.3, human [Q14847-1] |
Organism-specific databases
CTDi | 3927 |
DisGeNETi | 3927 |
GeneCardsi | LASP1 |
HGNCi | HGNC:6513, LASP1 |
HPAi | ENSG00000002834, Low tissue specificity |
MIMi | 602920, gene |
neXtProti | NX_Q14847 |
OpenTargetsi | ENSG00000002834 |
PharmGKBi | PA30298 |
VEuPathDBi | HostDB:ENSG00000002834 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1702, Eukaryota |
GeneTreei | ENSGT00940000154775 |
HOGENOMi | CLU_026811_0_1_1 |
InParanoidi | Q14847 |
OMAi | QESANYR |
PhylomeDBi | Q14847 |
TreeFami | TF319104 |
Enzyme and pathway databases
PathwayCommonsi | Q14847 |
SignaLinki | Q14847 |
SIGNORi | Q14847 |
Miscellaneous databases
BioGRID-ORCSi | 3927, 6 hits in 1041 CRISPR screens |
ChiTaRSi | LASP1, human |
EvolutionaryTracei | Q14847 |
GeneWikii | LASP1 |
GenomeRNAii | 3927 |
Pharosi | Q14847, Tbio |
PROi | PR:Q14847 |
RNActi | Q14847, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000002834, Expressed in upper lobe of lung and 251 other tissues |
ExpressionAtlasi | Q14847, baseline and differential |
Genevisiblei | Q14847, HS |
Family and domain databases
CDDi | cd11934, SH3_Lasp1_C, 1 hit |
InterProi | View protein in InterPro IPR035630, Lasp1_SH3 IPR000900, Nebulin_repeat IPR036028, SH3-like_dom_sf IPR001452, SH3_domain IPR001781, Znf_LIM |
Pfami | View protein in Pfam PF00412, LIM, 1 hit PF00880, Nebulin, 2 hits PF14604, SH3_9, 1 hit |
PRINTSi | PR00452, SH3DOMAIN |
SMARTi | View protein in SMART SM00132, LIM, 1 hit SM00227, NEBU, 2 hits SM00326, SH3, 1 hit |
SUPFAMi | SSF50044, SSF50044, 1 hit |
PROSITEi | View protein in PROSITE PS00478, LIM_DOMAIN_1, 1 hit PS50023, LIM_DOMAIN_2, 1 hit PS51216, NEBULIN, 2 hits PS50002, SH3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | LASP1_HUMAN | |
Accessioni | Q14847Primary (citable) accession number: Q14847 Secondary accession number(s): B4DGQ0, Q96ED2, Q96IG0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | January 1, 1998 | |
Last modified: | February 23, 2022 | |
This is version 203 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 17
Human chromosome 17: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references