UniProtKB - Q14802 (FXYD3_HUMAN)
FXYD domain-containing ion transport regulator 3
FXYD3
Functioni
Associates with and regulates the activity of the sodium/potassium-transporting ATPase (NKA) which transports Na+ out of the cell and K+ into the cell (PubMed:17077088).
Reduces glutathionylation of the NKA beta-1 subunit ATP1B1, thus reversing glutathionylation-mediated inhibition of ATP1B1 (PubMed:21454534).
Induces a hyperpolarization-activated chloride current when expressed in Xenopus oocytes (PubMed:7836447).
3 PublicationsDecreases the apparent K+ and Na+ affinity of the sodium/potassium-transporting ATPase over a large range of membrane potentials.
1 PublicationDecreases the apparent K+ affinity of the sodium/potassium-transporting ATPase only at slightly negative and positive membrane potentials and increases the apparent Na+ affinity over a large range of membrane potentials.
1 PublicationMiscellaneous
GO - Molecular functioni
- chloride channel activity Source: ProtInc
- sodium channel regulator activity Source: GO_Central
GO - Biological processi
- chloride transport Source: ProtInc
- potassium ion transport Source: UniProtKB-KW
- regulation of sodium ion transmembrane transporter activity Source: GO_Central
- sodium ion transport Source: UniProtKB-KW
Keywordsi
Biological process | Ion transport, Potassium transport, Sodium transport, Sodium/potassium transport, Transport |
Ligand | Potassium, Sodium |
Enzyme and pathway databases
PathwayCommonsi | Q14802 |
Reactomei | R-HSA-5578775, Ion homeostasis R-HSA-936837, Ion transport by P-type ATPases R-HSA-9679191, Potential therapeutics for SARS |
SignaLinki | Q14802 |
Protein family/group databases
TCDBi | 1.A.27.1.5, the phospholemman (plm) family |
Names & Taxonomyi
Protein namesi | Recommended name: FXYD domain-containing ion transport regulator 3Alternative name(s): Chloride conductance inducer protein Mat-8 Mammary tumor 8 kDa protein Phospholemman-like Sodium/potassium-transporting ATPase subunit FXYD3Curated |
Gene namesi | Name:FXYD3 Synonyms:MAT8, PLML |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:4027, FXYD3 |
MIMi | 604996, gene |
neXtProti | NX_Q14802 |
VEuPathDBi | HostDB:ENSG00000089356 |
Subcellular locationi
Plasma membrane
- Cell membrane Curated; Single-pass type I membrane protein Sequence analysis
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Plasma Membrane
- integral component of plasma membrane Source: ProtInc
- plasma membrane Source: Reactome
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 21 – 38 | ExtracellularSequence analysisAdd BLAST | 18 | |
Transmembranei | 39 – 59 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 60 – 87 | CytoplasmicSequence analysisAdd BLAST | 28 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 44 | C → S: Abolishes glutathionylation but does not affect interaction with ATP1B1; when associated with S-49; S-61 and S-63. 1 Publication | 1 | |
Mutagenesisi | 49 | C → S: Abolishes glutathionylation but does not affect interaction with ATP1B1; when associated with S-43; S-61 and S-63. 1 Publication | 1 | |
Mutagenesisi | 61 | C → S: Abolishes glutathionylation but does not affect interaction with ATP1B1; when associated with S-43; S-49 and S-63. 1 Publication | 1 | |
Mutagenesisi | 63 | C → S: Abolishes glutathionylation but does not affect interaction with ATP1B1; when associated with S-43; S-49 and S-61. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 5349 |
OpenTargetsi | ENSG00000089356 |
PharmGKBi | PA28443 |
Miscellaneous databases
Pharosi | Q14802, Tbio |
Genetic variation databases
BioMutai | FXYD3 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 20 | 1 PublicationAdd BLAST | 20 | |
ChainiPRO_0000010362 | 21 – 87 | FXYD domain-containing ion transport regulator 3Add BLAST | 67 |
Post-translational modificationi
Keywords - PTMi
GlutathionylationProteomic databases
jPOSTi | Q14802 |
MassIVEi | Q14802 |
MaxQBi | Q14802 |
PaxDbi | Q14802 |
PeptideAtlasi | Q14802 |
PRIDEi | Q14802 |
ProteomicsDBi | 25564 30673 60187 [Q14802-1] 60188 [Q14802-2] |
PTM databases
iPTMneti | Q14802 |
PhosphoSitePlusi | Q14802 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000089356, Expressed in mucosa of transverse colon and 193 other tissues |
ExpressionAtlasi | Q14802, baseline and differential |
Genevisiblei | Q14802, HS |
Organism-specific databases
HPAi | ENSG00000089356, Tissue enhanced (intestine) |
Interactioni
Subunit structurei
Regulatory subunit of the sodium/potassium-transporting ATPase which is composed of a catalytic alpha subunit, a non-catalytic beta subunit and an additional regulatory subunit (PubMed:17077088).
Interacts with catalytic alpha subunit ATP1A1 (PubMed:21454534).
Also interacts with non-catalytic beta subunit ATP1B1 (By similarity).
Interacts with the ATP1A1-ATP1B1, ATP1A2-ATP1B1 and ATP1A3-ATP1B1 NKA isozymes (PubMed:17077088).
By similarity2 PublicationsBinary interactionsi
Isoform 3 [Q14802-3]
Protein-protein interaction databases
BioGRIDi | 111364, 93 interactors |
IntActi | Q14802, 62 interactors |
MINTi | Q14802 |
STRINGi | 9606.ENSP00000473929 |
Miscellaneous databases
RNActi | Q14802, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 66 – 87 | DisorderedSequence analysisAdd BLAST | 22 |
Sequence similaritiesi
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502S9Z9, Eukaryota |
GeneTreei | ENSGT00940000153062 |
HOGENOMi | CLU_1781598_0_0_1 |
InParanoidi | Q14802 |
OMAi | IVLMSEW |
OrthoDBi | 1606794at2759 |
PhylomeDBi | Q14802 |
TreeFami | TF333443 |
Family and domain databases
InterProi | View protein in InterPro IPR000272, Ion-transport_regulator_FXYD |
Pfami | View protein in Pfam PF02038, ATP1G1_PLM_MAT8, 1 hit |
PROSITEi | View protein in PROSITE PS01310, FXYD, 1 hit |
s (5+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 5 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 5 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MQKVTLGLLV FLAGFPVLDA NDLEDKNSPF YYDWHSLQVG GLICAGVLCA
60 70 80
MGIIIVMSAK CKCKFGQKSG HHPGETPPLI TPGSAQS
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketS4R445 | S4R445_HUMAN | FXYD domain-containing ion transpor... | FXYD3 | 116 | Annotation score: | ||
S4R3M2 | S4R3M2_HUMAN | FXYD domain-containing ion transpor... | FXYD3 | 70 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 36 – 37 | Missing in AAA73922 (Ref. 2) Curated | 2 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_049109 | 40 | G → S. Corresponds to variant dbSNP:rs35578165Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_045716 | 1 | M → MGRGYSGALQARGGLEEPLE RGLRGPSFTQSPLHGAAAAY LSAQRDASLPVPGQRSDM in isoform 3. 1 Publication | 1 | |
Alternative sequenceiVSP_047286 | 33 – 87 | DWHSL…GSAQS → GESPCPLSPPHNPTYCLVPR VPIQGWGLT in isoform 5. CuratedAdd BLAST | 55 | |
Alternative sequenceiVSP_034598 | 58 | S → SEWRSSGEQAGRGWGSPPLT TQLSPTG in isoform 2 and isoform 4. 1 Publication | 1 | |
Alternative sequenceiVSP_047287 | 71 – 87 | Missing in isoform 4. CuratedAdd BLAST | 17 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X93036 mRNA Translation: CAA63604.1 U28249 mRNA Translation: AAA73922.1 BT006712 mRNA Translation: AAP35358.1 CR456945 mRNA Translation: CAG33226.1 CR542197 mRNA Translation: CAG46994.1 AC020907 Genomic DNA No translation available. BC005238 mRNA Translation: AAH05238.1 BC090044 mRNA Translation: AAH90044.1 BU157560 mRNA No translation available. |
CCDSi | CCDS12442.1 [Q14802-1] CCDS12443.1 [Q14802-2] CCDS46048.1 [Q14802-3] CCDS46049.1 [Q14802-4] CCDS46050.1 [Q14802-5] |
PIRi | A55571 |
RefSeqi | NP_001129479.1, NM_001136007.1 [Q14802-3] NP_001129480.1, NM_001136008.1 [Q14802-4] NP_001129481.1, NM_001136009.1 [Q14802-5] NP_001129482.1, NM_001136010.1 [Q14802-5] NP_001129483.1, NM_001136011.1 [Q14802-1] NP_001129484.1, NM_001136012.1 [Q14802-2] NP_005962.1, NM_005971.3 [Q14802-1] NP_068710.1, NM_021910.2 [Q14802-2] XP_005259053.1, XM_005258996.4 [Q14802-2] XP_016882366.1, XM_017026877.1 [Q14802-1] XP_016882367.1, XM_017026878.1 [Q14802-1] |
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X93036 mRNA Translation: CAA63604.1 U28249 mRNA Translation: AAA73922.1 BT006712 mRNA Translation: AAP35358.1 CR456945 mRNA Translation: CAG33226.1 CR542197 mRNA Translation: CAG46994.1 AC020907 Genomic DNA No translation available. BC005238 mRNA Translation: AAH05238.1 BC090044 mRNA Translation: AAH90044.1 BU157560 mRNA No translation available. |
CCDSi | CCDS12442.1 [Q14802-1] CCDS12443.1 [Q14802-2] CCDS46048.1 [Q14802-3] CCDS46049.1 [Q14802-4] CCDS46050.1 [Q14802-5] |
PIRi | A55571 |
RefSeqi | NP_001129479.1, NM_001136007.1 [Q14802-3] NP_001129480.1, NM_001136008.1 [Q14802-4] NP_001129481.1, NM_001136009.1 [Q14802-5] NP_001129482.1, NM_001136010.1 [Q14802-5] NP_001129483.1, NM_001136011.1 [Q14802-1] NP_001129484.1, NM_001136012.1 [Q14802-2] NP_005962.1, NM_005971.3 [Q14802-1] NP_068710.1, NM_021910.2 [Q14802-2] XP_005259053.1, XM_005258996.4 [Q14802-2] XP_016882366.1, XM_017026877.1 [Q14802-1] XP_016882367.1, XM_017026878.1 [Q14802-1] |
3D structure databases
SMRi | Q14802 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 111364, 93 interactors |
IntActi | Q14802, 62 interactors |
MINTi | Q14802 |
STRINGi | 9606.ENSP00000473929 |
Protein family/group databases
TCDBi | 1.A.27.1.5, the phospholemman (plm) family |
PTM databases
iPTMneti | Q14802 |
PhosphoSitePlusi | Q14802 |
Genetic variation databases
BioMutai | FXYD3 |
Proteomic databases
jPOSTi | Q14802 |
MassIVEi | Q14802 |
MaxQBi | Q14802 |
PaxDbi | Q14802 |
PeptideAtlasi | Q14802 |
PRIDEi | Q14802 |
ProteomicsDBi | 25564 30673 60187 [Q14802-1] 60188 [Q14802-2] |
Protocols and materials databases
Antibodypediai | 2266, 156 antibodies from 18 providers |
DNASUi | 5349 |
Genome annotation databases
Organism-specific databases
CTDi | 5349 |
DisGeNETi | 5349 |
GeneCardsi | FXYD3 |
HGNCi | HGNC:4027, FXYD3 |
HPAi | ENSG00000089356, Tissue enhanced (intestine) |
MIMi | 604996, gene |
neXtProti | NX_Q14802 |
OpenTargetsi | ENSG00000089356 |
PharmGKBi | PA28443 |
VEuPathDBi | HostDB:ENSG00000089356 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502S9Z9, Eukaryota |
GeneTreei | ENSGT00940000153062 |
HOGENOMi | CLU_1781598_0_0_1 |
InParanoidi | Q14802 |
OMAi | IVLMSEW |
OrthoDBi | 1606794at2759 |
PhylomeDBi | Q14802 |
TreeFami | TF333443 |
Enzyme and pathway databases
PathwayCommonsi | Q14802 |
Reactomei | R-HSA-5578775, Ion homeostasis R-HSA-936837, Ion transport by P-type ATPases R-HSA-9679191, Potential therapeutics for SARS |
SignaLinki | Q14802 |
Miscellaneous databases
BioGRID-ORCSi | 5349, 3 hits in 1027 CRISPR screens |
ChiTaRSi | FXYD3, human |
GeneWikii | FXYD3 |
GenomeRNAii | 5349 |
Pharosi | Q14802, Tbio |
PROi | PR:Q14802 |
RNActi | Q14802, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000089356, Expressed in mucosa of transverse colon and 193 other tissues |
ExpressionAtlasi | Q14802, baseline and differential |
Genevisiblei | Q14802, HS |
Family and domain databases
InterProi | View protein in InterPro IPR000272, Ion-transport_regulator_FXYD |
Pfami | View protein in Pfam PF02038, ATP1G1_PLM_MAT8, 1 hit |
PROSITEi | View protein in PROSITE PS01310, FXYD, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | FXYD3_HUMAN | |
Accessioni | Q14802Primary (citable) accession number: Q14802 Secondary accession number(s): A6NDE0 Q6IB59 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 182 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families