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Protein

Clathrin interactor 1

Gene

CLINT1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). May have a role in transport via clathrin-coated vesicles from the trans-Golgi network to endosomes. Stimulates clathrin assembly.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei29Phosphatidylinositol lipid headgroupBy similarity1
Binding sitei67Phosphatidylinositol lipid headgroupBy similarity1

GO - Molecular functioni

  • cadherin binding Source: BHF-UCL
  • clathrin binding Source: BHF-UCL
  • lipid binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Biological processEndocytosis
LigandLipid-binding

Enzyme and pathway databases

ReactomeiR-HSA-432722 Golgi Associated Vesicle Biogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Clathrin interactor 1
Alternative name(s):
Clathrin-interacting protein localized in the trans-Golgi region
Short name:
Clint
Enthoprotin
Epsin-4
Epsin-related protein
Short name:
EpsinR
Gene namesi
Name:CLINT1
Synonyms:ENTH, EPN4, EPNR, KIAA0171
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000113282.13
HGNCiHGNC:23186 CLINT1
MIMi607265 gene
neXtProtiNX_Q14677

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi29R → L: Reduces lipid binding. Abolishes lipid binding; when associated with G-34. 1
Mutagenesisi34D → G: Abolishes lipid binding; when associated with L-29. 1 Publication1
Mutagenesisi41G → S: Normal binding to VTI1B. 1 Publication1
Mutagenesisi46E → W: Normal binding to VTI1B. 1 Publication1
Mutagenesisi52F → D: Abolished binding to VTI1B. 1 Publication1
Mutagenesisi53 – 54MY → DD: Abolished binding to VTI1B. 1 Publication2
Mutagenesisi95E → W: Normal binding to VTI1B. 1 Publication1
Mutagenesisi96R → S: Abolished binding to VTI1B. 1 Publication1
Mutagenesisi146R → E: Abolished binding to VTI1B. Rescued binding to VTI1B R-23 mutant. 1 Publication1
Mutagenesisi153K → D: Normal binding to VTI1B. 1 Publication1
Mutagenesisi159Y → S: Normal binding to VTI1B. 1 Publication1
Mutagenesisi349D → R: Decreases AP-1 and AP-2 binding. 1 Publication1
Mutagenesisi371D → R: Slightly decreases AP-1 binding. 1 Publication1
Mutagenesisi422D → R: Strongly decreases clathrin binding. 1 Publication1
Mutagenesisi423 – 426LFDL → AFAA: Strongly reduces clathrin binding. 1 Publication4

Organism-specific databases

DisGeNETi9685
OpenTargetsiENSG00000113282
PharmGKBiPA145149115

Polymorphism and mutation databases

BioMutaiCLINT1
DMDMi41016993

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000745211 – 625Clathrin interactor 1Add BLAST625

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei163PhosphoserineBy similarity1
Modified residuei166PhosphoserineCombined sources1
Modified residuei173PhosphoserineCombined sources1
Modified residuei205PhosphoserineBy similarity1
Modified residuei210PhosphoserineCombined sources1
Modified residuei227PhosphoserineCombined sources1
Modified residuei245PhosphoserineCombined sources1
Modified residuei299PhosphoserineCombined sources1
Modified residuei308PhosphothreonineCombined sources1
Modified residuei312PhosphoserineCombined sources1
Modified residuei624PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ14677
MaxQBiQ14677
PaxDbiQ14677
PeptideAtlasiQ14677
PRIDEiQ14677
ProteomicsDBi60109
60110 [Q14677-2]
60111 [Q14677-3]

PTM databases

iPTMnetiQ14677
PhosphoSitePlusiQ14677

Expressioni

Tissue specificityi

Ubiquitously expressed at low to intermediate levels.2 Publications

Gene expression databases

BgeeiENSG00000113282 Expressed in 233 organ(s), highest expression level in oviduct epithelium
CleanExiHS_CLINT1
ExpressionAtlasiQ14677 baseline and differential
GenevisibleiQ14677 HS

Organism-specific databases

HPAiHPA043280
HPA056947

Interactioni

Subunit structurei

Binds clathrin heavy chain, GGA2, AP-2 and AP1G1 (PubMed:12213833, PubMed:12429846, PubMed:12538641). Interacts with VTI1B (PubMed:18033301).4 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi115038, 104 interactors
DIPiDIP-45604N
ELMiQ14677
IntActiQ14677, 79 interactors
MINTiQ14677
STRINGi9606.ENSP00000429824

Structurei

Secondary structure

1625
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ14677
SMRiQ14677
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ14677

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini16 – 149ENTHPROSITE-ProRule annotationAdd BLAST134

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni52 – 54Interaction with VTI1BCombined sources1 Publication3
Regioni94 – 96Interaction with VTI1BCombined sources1 Publication3
Regioni142 – 153Interaction with VTI1BCombined sources1 PublicationAdd BLAST12

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi256 – 278Thr-richPROSITE-ProRule annotationAdd BLAST23
Compositional biasi311 – 485Ser-richPROSITE-ProRule annotationAdd BLAST175
Compositional biasi549 – 605Met-richPROSITE-ProRule annotationAdd BLAST57

Sequence similaritiesi

Belongs to the epsin family.Curated

Phylogenomic databases

eggNOGiKOG2056 Eukaryota
ENOG410XSM0 LUCA
GeneTreeiENSGT00550000074611
HOGENOMiHOG000082415
HOVERGENiHBG048921
InParanoidiQ14677
OMAiNMNIGMS
OrthoDBiEOG091G0FWU
PhylomeDBiQ14677
TreeFamiTF313361

Family and domain databases

CDDicd03571 ENTH_epsin, 1 hit
Gene3Di1.25.40.90, 1 hit
InterProiView protein in InterPro
IPR030544 CLINT1
IPR013809 ENTH
IPR008942 ENTH_VHS
IPR039416 Epsin_ENTH
PANTHERiPTHR12276:SF67 PTHR12276:SF67, 1 hit
PfamiView protein in Pfam
PF01417 ENTH, 1 hit
SMARTiView protein in SMART
SM00273 ENTH, 1 hit
SUPFAMiSSF48464 SSF48464, 1 hit
PROSITEiView protein in PROSITE
PS50942 ENTH, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q14677-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MLNMWKVREL VDKATNVVMN YSEIESKVRE ATNDDPWGPS GQLMGEIAKA
60 70 80 90 100
TFMYEQFPEL MNMLWSRMLK DNKKNWRRVY KSLLLLAYLI RNGSERVVTS
110 120 130 140 150
AREHIYDLRS LENYHFVDEH GKDQGINIRQ KVKELVEFAQ DDDRLREERK
160 170 180 190 200
KAKKNKDKYV GVSSDSVGGF RYSERYDPEP KSKWDEEWDK NKSAFPFSDK
210 220 230 240 250
LGELSDKIGS TIDDTISKFR RKDREDSPER CSDSDEEKKA RRGRSPKGEF
260 270 280 290 300
KDEEETVTTK HIHITQATET TTTRHKRTAN PSKTIDLGAA AHYTGDKASP
310 320 330 340 350
DQNASTHTPQ SSVKTSVPSS KSSGDLVDLF DGTSQSTGGS ADLFGGFADF
360 370 380 390 400
GSAAASGSFP SQVTATSGNG DFGDWSAFNQ APSGPVASSG EFFGSASQPA
410 420 430 440 450
VELVSGSQSA LGPPPAASNS SDLFDLMGSS QATMTSSQSM NFSMMSTNTV
460 470 480 490 500
GLGLPMSRSQ NTDMVQKSVS KTLPSTWSDP SVNISLDNLL PGMQPSKPQQ
510 520 530 540 550
PSLNTMIQQQ NMQQPMNVMT QSFGAVNLSS PSNMLPVRPQ TNALIGGPMP
560 570 580 590 600
MSMPNVMTGT MGMAPLGNTP MMNQSMMGMN MNIGMSAAGM GLTGTMGMGM
610 620
PNIAMTSGTV QPKQDAFANF ANFSK
Length:625
Mass (Da):68,259
Last modified:November 1, 1996 - v1
Checksum:i6C8C6689861E9F0D
GO
Isoform 2 (identifier: Q14677-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-18: Missing.
     460-460: Q → QPLQNVSTVLQKPNPLYNQ

Show »
Length:625
Mass (Da):68,166
Checksum:i5081B36E2F4F85F2
GO
Isoform 3 (identifier: Q14677-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     459-459: S → SQPLQNVSTVLQKPNPLYN

Note: No experimental confirmation available.
Show »
Length:643
Mass (Da):70,295
Checksum:iFD6381C2E1957E9C
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H0YCL3H0YCL3_HUMAN
Clathrin interactor 1
CLINT1
55Annotation score:
H0YD52H0YD52_HUMAN
Clathrin interactor 1
CLINT1
133Annotation score:
H0YDU9H0YDU9_HUMAN
Clathrin interactor 1
CLINT1
66Annotation score:

Sequence cautioni

The sequence BAA11488 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti476T → A in BAC03971 (PubMed:14702039).Curated1

Polymorphismi

Genetic variations in CLINT1 may contribute to susceptibility to schizophrenia (SCZD1) and psychotic disorders in some populations.1 Publication

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0091601 – 18Missing in isoform 2. 2 PublicationsAdd BLAST18
Alternative sequenceiVSP_043302459S → SQPLQNVSTVLQKPNPLYN in isoform 3. 1 Publication1
Alternative sequenceiVSP_009161460Q → QPLQNVSTVLQKPNPLYNQ in isoform 2. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BK000414 Genomic DNA Translation: DAA00062.1
AF434813 mRNA Translation: AAL30768.1
D79993 mRNA Translation: BAA11488.2 Different initiation.
AK092765 mRNA Translation: BAC03971.1
AK300257 mRNA Translation: BAH13244.1
AC011394 Genomic DNA No translation available.
AC026407 Genomic DNA No translation available.
CH471062 Genomic DNA Translation: EAW61585.1
CH471062 Genomic DNA Translation: EAW61588.1
BC004467 mRNA Translation: AAH04467.1
BC013091 mRNA Translation: AAH13091.1
CCDSiCCDS47330.1 [Q14677-1]
CCDS56388.1 [Q14677-2]
CCDS56389.1 [Q14677-3]
RefSeqiNP_001182484.1, NM_001195555.1 [Q14677-3]
NP_001182485.1, NM_001195556.1 [Q14677-2]
NP_055481.1, NM_014666.3 [Q14677-1]
UniGeneiHs.644000

Genome annotation databases

EnsembliENST00000411809; ENSP00000388340; ENSG00000113282 [Q14677-1]
ENST00000523094; ENSP00000429345; ENSG00000113282 [Q14677-2]
ENST00000523908; ENSP00000429824; ENSG00000113282 [Q14677-3]
ENST00000530742; ENSP00000433419; ENSG00000113282 [Q14677-2]
GeneIDi9685
KEGGihsa:9685
UCSCiuc003lxi.3 human [Q14677-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BK000414 Genomic DNA Translation: DAA00062.1
AF434813 mRNA Translation: AAL30768.1
D79993 mRNA Translation: BAA11488.2 Different initiation.
AK092765 mRNA Translation: BAC03971.1
AK300257 mRNA Translation: BAH13244.1
AC011394 Genomic DNA No translation available.
AC026407 Genomic DNA No translation available.
CH471062 Genomic DNA Translation: EAW61585.1
CH471062 Genomic DNA Translation: EAW61588.1
BC004467 mRNA Translation: AAH04467.1
BC013091 mRNA Translation: AAH13091.1
CCDSiCCDS47330.1 [Q14677-1]
CCDS56388.1 [Q14677-2]
CCDS56389.1 [Q14677-3]
RefSeqiNP_001182484.1, NM_001195555.1 [Q14677-3]
NP_001182485.1, NM_001195556.1 [Q14677-2]
NP_055481.1, NM_014666.3 [Q14677-1]
UniGeneiHs.644000

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XGWX-ray1.90A1-165[»]
2QY7X-ray2.00A/B/C20-166[»]
2V8SX-ray2.22E20-166[»]
ProteinModelPortaliQ14677
SMRiQ14677
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115038, 104 interactors
DIPiDIP-45604N
ELMiQ14677
IntActiQ14677, 79 interactors
MINTiQ14677
STRINGi9606.ENSP00000429824

PTM databases

iPTMnetiQ14677
PhosphoSitePlusiQ14677

Polymorphism and mutation databases

BioMutaiCLINT1
DMDMi41016993

Proteomic databases

EPDiQ14677
MaxQBiQ14677
PaxDbiQ14677
PeptideAtlasiQ14677
PRIDEiQ14677
ProteomicsDBi60109
60110 [Q14677-2]
60111 [Q14677-3]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000411809; ENSP00000388340; ENSG00000113282 [Q14677-1]
ENST00000523094; ENSP00000429345; ENSG00000113282 [Q14677-2]
ENST00000523908; ENSP00000429824; ENSG00000113282 [Q14677-3]
ENST00000530742; ENSP00000433419; ENSG00000113282 [Q14677-2]
GeneIDi9685
KEGGihsa:9685
UCSCiuc003lxi.3 human [Q14677-1]

Organism-specific databases

CTDi9685
DisGeNETi9685
EuPathDBiHostDB:ENSG00000113282.13
GeneCardsiCLINT1
HGNCiHGNC:23186 CLINT1
HPAiHPA043280
HPA056947
MIMi607265 gene
neXtProtiNX_Q14677
OpenTargetsiENSG00000113282
PharmGKBiPA145149115
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2056 Eukaryota
ENOG410XSM0 LUCA
GeneTreeiENSGT00550000074611
HOGENOMiHOG000082415
HOVERGENiHBG048921
InParanoidiQ14677
OMAiNMNIGMS
OrthoDBiEOG091G0FWU
PhylomeDBiQ14677
TreeFamiTF313361

Enzyme and pathway databases

ReactomeiR-HSA-432722 Golgi Associated Vesicle Biogenesis

Miscellaneous databases

ChiTaRSiCLINT1 human
EvolutionaryTraceiQ14677
GeneWikiiCLINT1
GenomeRNAii9685
PROiPR:Q14677
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000113282 Expressed in 233 organ(s), highest expression level in oviduct epithelium
CleanExiHS_CLINT1
ExpressionAtlasiQ14677 baseline and differential
GenevisibleiQ14677 HS

Family and domain databases

CDDicd03571 ENTH_epsin, 1 hit
Gene3Di1.25.40.90, 1 hit
InterProiView protein in InterPro
IPR030544 CLINT1
IPR013809 ENTH
IPR008942 ENTH_VHS
IPR039416 Epsin_ENTH
PANTHERiPTHR12276:SF67 PTHR12276:SF67, 1 hit
PfamiView protein in Pfam
PF01417 ENTH, 1 hit
SMARTiView protein in SMART
SM00273 ENTH, 1 hit
SUPFAMiSSF48464 SSF48464, 1 hit
PROSITEiView protein in PROSITE
PS50942 ENTH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiEPN4_HUMAN
AccessioniPrimary (citable) accession number: Q14677
Secondary accession number(s): B7Z6F8
, D3DQJ6, Q8NAF1, Q96E05
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: November 1, 1996
Last modified: November 7, 2018
This is version 181 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
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