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UniProtKB - Q14653 (IRF3_HUMAN)

Entry version 207 (25 May 2022)
Sequence version 1 (01 Nov 1997)
Previous versions | rss
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Protein

Interferon regulatory factor 3

Gene

IRF3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses (PubMed:22394562, PubMed:25636800, PubMed:27302953).

Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters (PubMed:11846977, PubMed:16846591, PubMed:16979567, PubMed:20049431, PubMed:32972995).

Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction (PubMed:16846591, PubMed:16979567, PubMed:20049431).

Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases (PubMed:22394562, PubMed:25636800, PubMed:27302953).

This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes (PubMed:16154084, PubMed:27302953, PubMed:33440148).

Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages (PubMed:16846591).

In response to Sendai virus infection, is recruited by TOMM70:HSP90AA1 to mitochondrion and forms an apoptosis complex TOMM70:HSP90AA1:IRF3:BAX inducing apoptosis (PubMed:25609812).

Key transcription factor regulating the IFN response during SARS-CoV-2 infection (PubMed:33440148).

4 Publications8 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

In the absence of viral infection, maintained as a monomer in an autoinhibited state (PubMed:16846591, PubMed:16979567, PubMed:20049431). Phosphorylation by TBK1 and IKBKE disrupts this autoinhibition leading to the liberation of the DNA-binding and dimerization activities and its nuclear localization where it can activate type I IFN and ISG genes (PubMed:25636800). Phosphorylation and activation follow the following steps: innate adapter protein MAVS, STING1 or TICAM1 are first activated by viral RNA, cytosolic DNA and bacterial lipopolysaccharide (LPS), respectively, leading to activation of the kinases TBK1 and IKBKE (PubMed:25636800). These kinases then phosphorylate the adapter proteins on their pLxIS motif, leading to recruitment of IRF3, thereby licensing IRF3 for phosphorylation by TBK1 (PubMed:25636800). Phosphorylated IRF3 dissociates from the adapter proteins, dimerizes, and then enters the nucleus to induce IFNs (PubMed:25636800, PubMed:27302953).3 Publications3 Publications
(Microbial infection) Activated upon coronavirus SARS-CoV-2 infection.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section specifies the position and type of each DNA-binding domain present within the protein.<p><a href='/help/dna_bind' target='_top'>More...</a></p>DNA bindingi5 – 111IRF tryptophan pentad repeatPROSITE-ProRule annotationAdd BLAST107

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionActivator, DNA-binding
Biological processAntiviral defense, Host-virus interaction, Immunity, Innate immunity, Transcription, Transcription regulation

Enzyme and pathway databases

Pathway Commons web resource for biological pathway data

More...PathwayCommonsi		Q14653

Reactome - a knowledgebase of biological pathways and processes

More...Reactomei		R-HSA-1169408, ISG15 antiviral mechanism
R-HSA-1606341, IRF3 mediated activation of type 1 IFN
R-HSA-168928, DDX58/IFIH1-mediated induction of interferon-alpha/beta
R-HSA-3134973, LRR FLII-interacting protein 1 (LRRFIP1) activates type I IFN production
R-HSA-3134975, Regulation of innate immune responses to cytosolic DNA
R-HSA-3270619, IRF3-mediated induction of type I IFN
R-HSA-877300, Interferon gamma signaling
R-HSA-9013973, TICAM1-dependent activation of IRF3/IRF7
R-HSA-909733, Interferon alpha/beta signaling
R-HSA-918233, TRAF3-dependent IRF activation pathway
R-HSA-933541, TRAF6 mediated IRF7 activation
R-HSA-936440, Negative regulators of DDX58/IFIH1 signaling
R-HSA-936964, Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon

SignaLink: a signaling pathway resource with multi-layered regulatory networks

More...SignaLinki		Q14653

SIGNOR Signaling Network Open Resource

More...SIGNORi		Q14653

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Interferon regulatory factor 32 Publications
Short name:
IRF-31 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:IRF31 PublicationImported
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 19

Organism-specific databases

Human Gene Nomenclature Database

More...HGNCi		HGNC:6118, IRF3

Online Mendelian Inheritance in Man (OMIM)

More...MIMi		603734, gene

neXtProt; the human protein knowledge platform

More...neXtProti		NX_Q14653

Eukaryotic Pathogen, Vector and Host Database Resources

More...VEuPathDBi		HostDB:ENSG00000126456

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section provides information on the disease(s) associated with genetic variations in a given protein. The information is extracted from the scientific literature and diseases that are also described in the <a href="http://www.ncbi.nlm.nih.gov/sites/entrez?db=omim">OMIM</a> database are represented with a <a href="http://www.uniprot.org/diseases">controlled vocabulary</a> in the following way:<p><a href='/help/involvement_in_disease' target='_top'>More...</a></p>Involvement in diseasei

Encephalopathy, acute, infection-induced, 7, herpes-specific (IIAE7)1 Publication
Disease susceptibility is associated with variants affecting the gene represented in this entry.
Disease descriptionA rare complication of human herpesvirus 1 (HHV-1) infection, occurring in only a small minority of HHV-1 infected individuals. It is characterized by hemorrhagic necrosis of parts of the temporal and frontal lobes. Onset is over several days and involves fever, headache, seizures, stupor, and often coma, frequently with a fatal outcome.
Related information in OMIM
Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_075805285R → Q in IIAE7; loss of viral infection-induced phosphorylation at S-386; loss of viral infection-induced homodimerization; loss of viral infection-induced transcription factor activity; unable to activate interferon transcription in response to viral infection; decreased IFNB induction upon Sendai virus infection. 2 PublicationsCorresponds to variant dbSNP:rs750526659EnsemblClinVar.1

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi77 – 78KR → NG: Abolishes nuclear localization. 1 Publication2
Mutagenesisi86 – 87RK → LQ: No effect on subcellular localization. 1 Publication2
Mutagenesisi116D → A: Does not affect cleavage by CASP3. 1 Publication1
Mutagenesisi121 – 125DTSPD → ATSPA: Abolished cleavage by CASP3. 1 Publication5
Mutagenesisi139 – 140IL → MM: Abolishes nuclear export. 1 Publication2
Mutagenesisi193K → R: Highly diminished ISGylation; when associated with R-360 and R-366. 1 Publication1
Mutagenesisi285R → S: Abolished interaction with STING1, MAVS or TICAM1. 1 Publication1
Mutagenesisi288H → S: Decreased interaction with TICAM1. 1 Publication1
Mutagenesisi290H → S: Decreased interaction with TICAM1. 1 Publication1
Mutagenesisi313K → S: Abolished interaction with STING1, MAVS or TICAM1. 1 Publication1
Mutagenesisi360K → R: Highly diminished ISGylation; when associated with R-193 and R-366. 1 Publication1
Mutagenesisi366K → R: Highly diminished ISGylation; when associated with R-193 and R-360. 1 Publication1
Mutagenesisi385 – 386SS → AA: Complete loss of viral infection induced phosphorylation. 1 Publication2
Mutagenesisi385S → A, D or E: Complete loss of viral infection induced phosphorylation. 3 Publications1
Mutagenesisi386S → A, D or E: Complete loss of viral infection induced phosphorylation. 4 Publications1
Mutagenesisi386S → E: Phosphomimetic mutant; interacts with CREBBP; when associated with E-396. 1 Publication1
Mutagenesisi396 – 405SNSHPLSLTS → ANAHPLALAA: Complete loss of viral infection induced phosphorylation. 1 Publication10
Mutagenesisi396 – 405SNSHPLSLTS → DNDHPLDLDD: Acts as a constitutively activated IRF3. 1 Publication10
Mutagenesisi396 – 398SNS → ANA: Complete loss of viral infection induced phosphorylation. 3
Mutagenesisi396S → E: Phosphomimetic mutant; interacts with CREBBP; when associated with E-386. 1 Publication1
Mutagenesisi402 – 405SLTS → ALAA: Complete loss of viral infection induced phosphorylation. 4

Keywords - Diseasei

Disease variant

Organism-specific databases

DisGeNET

More...DisGeNETi		3661

MalaCards human disease database

More...MalaCardsi		IRF3
MIMi616532, phenotype

Open Targets

More...OpenTargetsi		ENSG00000126456

The Pharmacogenetics and Pharmacogenomics Knowledge Base

More...PharmGKBi		PA29917

Miscellaneous databases

Pharos NIH Druggable Genome Knowledgebase

More...Pharosi		Q14653, Tbio

Chemistry databases

ChEMBL database of bioactive drug-like small molecules

More...ChEMBLi		CHEMBL4523293

Genetic variation databases

BioMuta curated single-nucleotide variation and disease association database

More...BioMutai		IRF3

Domain mapping of disease mutations (DMDM)

More...DMDMi		2497442

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001545531 – 427Interferon regulatory factor 3Add BLAST427

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei3Phosphothreonine1 Publication1
Modified residuei14Phosphoserine1 Publication1
Modified residuei75Phosphothreonine1 Publication1
Modified residuei97Phosphoserine1 Publication1
Modified residuei123PhosphoserineBy similarity1
Modified residuei180Phosphothreonine1 Publication1
Modified residuei188Phosphoserine1 Publication1
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM / Processing</a> section describes <strong>covalent linkages</strong> of various types formed <strong>between two proteins (interchain cross-links)</strong> or <strong>between two parts of the same protein (intrachain cross-links)</strong>, except the disulfide bonds that are annotated in the <a href="http://www.uniprot.org/manual/disulfid">'Disulfide bond'</a> subsection.<p><a href='/help/crosslnk' target='_top'>More...</a></p>Cross-linki193Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ISG15)1 Publication
Modified residuei237Phosphothreonine1 Publication1
Modified residuei244Phosphothreonine1 Publication1
Modified residuei253Phosphothreonine1 Publication1
<p>This subsection of the PTM / Processing":/help/ptm_processing_section section describes the positions of cysteine residues participating in disulfide bonds.<p><a href='/help/disulfid' target='_top'>More...</a></p>Disulfide bondi267 ↔ 2892 Publications
Cross-linki360Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ISG15)1 Publication
Cross-linki366Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ISG15)1 Publication
Modified residuei385Phosphoserine1 Publication1
Modified residuei386Phosphoserine; by TBK13 Publications1
Modified residuei396Phosphoserine; by IKKE and TBK13 Publications1
Modified residuei398Phosphoserine1 Publication1
Modified residuei404Phosphothreonine1 Publication1
Modified residuei427Phosphoserine1 Publication1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Constitutively phosphorylated on many Ser/Thr residues (PubMed:22394562, PubMed:23478265, PubMed:23746807). Activated following phosphorylation by TBK1 and IKBKE (PubMed:23478265, PubMed:23746807, PubMed:25636800). Innate adapter protein MAVS, STING1 or TICAM1 are first activated by viral RNA, cytosolic DNA, and bacterial lipopolysaccharide (LPS), respectively, leading to activation of the kinases TBK1 and IKBKE (PubMed:25636800). These kinases then phosphorylate the adapter proteins on the pLxIS motif, leading to recruitment of IRF3, thereby licensing IRF3 for phosphorylation by TBK1 (PubMed:25636800). Phosphorylated IRF3 dissociates from the adapter proteins, dimerizes, and then enters the nucleus to induce IFNs (PubMed:25636800).4 Publications
Ubiquitinated; ubiquitination involves RBCK1 leading to proteasomal degradation (PubMed:18711448). Polyubiquitinated; ubiquitination involves TRIM21 leading to proteasomal degradation (PubMed:18641315). Ubiquitinated by UBE3C, leading to its degradation (PubMed:21167755).3 Publications
ISGylated by HERC5 resulting in sustained IRF3 activation and in the inhibition of IRF3 ubiquitination by disrupting PIN1 binding. The phosphorylation state of IRF3 does not alter ISGylation.3 Publications
Proteolytically cleaved by apoptotic caspases during apoptosis, leading to its inactivation (PubMed:30878284). Cleavage by CASP3 during virus-induced apoptosis inactivates it, preventing cytokine overproduction (PubMed:30878284).1 Publication
(Microbial infection) ISGylated. ISGylation is cleaved and removed by SARS-COV-2 nsp3 which attenuates type I interferon responses.1 Publication
(Microbial infection) Phosphorylation and subsequent activation of IRF3 is inhibited by vaccinia virus protein E3.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections ('Function', 'PTM / Processing', 'Pathology and Biotech') according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei121 – 122Cleavage; by CASP31 Publication2
Sitei125 – 126Cleavage; by CASP31 Publication2

Keywords - PTMi

Disulfide bond, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

Encyclopedia of Proteome Dynamics

More...EPDi		Q14653

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		Q14653

MassIVE - Mass Spectrometry Interactive Virtual Environment

More...MassIVEi		Q14653

MaxQB - The MaxQuant DataBase

More...MaxQBi		Q14653

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		Q14653

PeptideAtlas

More...PeptideAtlasi		Q14653

PRoteomics IDEntifications database

More...PRIDEi		Q14653

ProteomicsDB: a multi-organism proteome resource

More...ProteomicsDBi		60091 [Q14653-1]
60092 [Q14653-2]
62791

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		Q14653

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...PhosphoSitePlusi		Q14653

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Expressed constitutively in a variety of tissues.

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...Bgeei		ENSG00000126456, Expressed in granulocyte and 222 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...ExpressionAtlasi		Q14653, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...Genevisiblei		Q14653, HS

Organism-specific databases

Human Protein Atlas

More...HPAi		ENSG00000126456, Low tissue specificity

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Monomer (PubMed:16846591, PubMed:16979567, PubMed:20049431). Homodimer; phosphorylation-induced (PubMed:22394562, PubMed:25636800, PubMed:26347139).

Interacts (when phosphorylated) with CREBBP (PubMed:16154084, PubMed:27302953).

Interacts with MAVS (via phosphorylated pLxIS motif) (PubMed:16153868, PubMed:25636800, PubMed:27302953).

Interacts with TICAM1 (via phosphorylated pLxIS motif) (PubMed:12471095, PubMed:14739303, PubMed:25636800, PubMed:27302953).

Interacts with STING1 (via phosphorylated pLxIS motif) (PubMed:22394562, PubMed:28331227, PubMed:25636800, PubMed:27302953).

Interacts with IKBKE and TBK1 (PubMed:16281057, PubMed:23478265, PubMed:25636800).

Interacts with TICAM2 (PubMed:14517278).

Interacts with RBCK1 (PubMed:18711448).

Interacts with HERC5 (PubMed:20308324).

Interacts with DDX3X (phosphorylated at 'Ser-102'); the interaction allows the phosphorylation and activation of IRF3 by IKBKE (PubMed:23478265, PubMed:27980081).

Interacts with TRIM21 and ULK1, in the presence of TRIM21; this interaction leads to IRF3 degradation by autophagy (PubMed:18641315, PubMed:26347139).

Interacts with RIOK3; RIOK3 probably mediates the interaction of TBK1 with IRF3 (PubMed:19557502).

Interacts with ILRUN; the interaction inhibits IRF3 binding to its DNA consensus sequence (PubMed:29802199).

Interacts with LYAR; this interaction impairs IRF3 DNA-binding activity (PubMed:31413131).

Interacts with TRAF3 (PubMed:27980081).

Interacts with ZDHHC11; ZDHHC11 recruits IRF3 to STING1 upon DNA virus infection and thereby promotes IRF3 activation (PubMed:28331227).

Interacts with HSP90AA1; the interaction mediates IRF3 association with TOMM70 (PubMed:20628368, PubMed:25609812).

Interacts with BCL2; the interaction decreases upon Sendai virus infection (PubMed:25609812).

Interacts with BAX; the interaction is direct, increases upon Sendai virus infection and mediates the formation of the apoptosis complex TOMM70:HSP90AA1:IRF3:BAX (PubMed:25609812).

3 Publications21 Publications

(Microbial infection) Interacts with rotavirus A NSP1 (via pLxIS motif); this interaction leads to the proteasome-dependent degradation of IRF3.

2 Publications

(Microbial infection) Interacts with herpes virus 8/HHV-8 protein VIRF1 (PubMed:11314014).

1 Publication

(Microbial infection) Interacts with Seneca Valley virus protease 3C; this interaction is involved in the suppression of IRF3 expression and phosphorylation by the virus.

1 Publication

(Microbial infection) Interacts with herpes virus 2/HHV-2 protein ICP27; this interaction inhibits IRF3 phosphorylation and nuclear translocation.

1 Publication

(Microbial infection) Interacts with human cytomegalovirus protein UL44; this interaction prevents IRF3 binding to its promoters.

1 Publication

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Hide details

GO - Molecular functioni

Complete GO annotation on QuickGO ...

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		109869, 123 interactors

CORUM comprehensive resource of mammalian protein complexes

More...CORUMi		Q14653

Database of interacting proteins

More...DIPi		DIP-41448N

The Eukaryotic Linear Motif resource for Functional Sites in Proteins

More...ELMi		Q14653

Protein interaction database and analysis system

More...IntActi		Q14653, 71 interactors

Molecular INTeraction database

More...MINTi		Q14653

STRING: functional protein association networks

More...STRINGi		9606.ENSP00000471896

Chemistry databases

BindingDB database of measured binding affinities

More...BindingDBi		Q14653

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...RNActi		Q14653, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1427
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

AlphaFold Protein Structure Database

More...AlphaFoldDBi		Q14653

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		Q14653

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...EvolutionaryTracei		Q14653

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni91 – 136DisorderedSequence analysisAdd BLAST46
Regioni141 – 427Mediates interaction with ZDHHC111 PublicationAdd BLAST287
Regioni200 – 360Interaction with HERC51 PublicationAdd BLAST161

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.<p><a href='/help/motif' target='_top'>More...</a></p>Motifi139 – 149Nuclear export signalAdd BLAST11

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular type of amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi91 – 106Basic and acidic residuesSequence analysisAdd BLAST16
Compositional biasi115 – 136Polar residuesSequence analysisAdd BLAST22

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the IRF family.PROSITE-ProRule annotation

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		ENOG502QTRR, Eukaryota

Ensembl GeneTree

More...GeneTreei		ENSGT00940000160569

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_031544_2_0_1

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		Q14653

Identification of Orthologs from Complete Genome Data

More...OMAi		CHTYWAM

Database of Orthologous Groups

More...OrthoDBi		648909at2759

Database for complete collections of gene phylogenies

More...PhylomeDBi		Q14653

TreeFam database of animal gene trees

More...TreeFami		TF328512

Family and domain databases

Conserved Domains Database

More...CDDi		cd00103, IRF, 1 hit

Database of protein disorder

More...DisProti		DP01614

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		1.10.10.10, 1 hit
2.60.200.10, 1 hit

Intrinsically Disordered proteins with Extensive Annotations and Literature

More...IDEALi		IID00079

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR019817, Interferon_reg_fac_CS
IPR001346, Interferon_reg_fact_DNA-bd_dom
IPR019471, Interferon_reg_factor-3
IPR017855, SMAD-like_dom_sf
IPR008984, SMAD_FHA_dom_sf
IPR036388, WH-like_DNA-bd_sf
IPR036390, WH_DNA-bd_sf

The PANTHER Classification System

More...PANTHERi		PTHR11949, PTHR11949, 1 hit

Pfam protein domain database

More...Pfami		View protein in Pfam
PF00605, IRF, 1 hit
PF10401, IRF-3, 1 hit

Protein Motif fingerprint database; a protein domain database

More...PRINTSi		PR00267, INTFRNREGFCT

Simple Modular Architecture Research Tool; a protein domain database

More...SMARTi		View protein in SMART
SM00348, IRF, 1 hit
SM01243, IRF-3, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF46785, SSF46785, 1 hit
SSF49879, SSF49879, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS00601, IRF_1, 1 hit
PS51507, IRF_2, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (5+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 5 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 5 described isoforms and 13 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q14653-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <p><strong>What is the canonical sequence?</strong><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGTPKPRILP WLVSQLDLGQ LEGVAWVNKS RTRFRIPWKH GLRQDAQQED 
        60         70         80         90        100
FGIFQAWAEA TGAYVPGRDK PDLPTWKRNF RSALNRKEGL RLAEDRSKDP 
       110        120        130        140        150
HDPHKIYEFV NSGVGDFSQP DTSPDTNGGG STSDTQEDIL DELLGNMVLA 
       160        170        180        190        200
PLPDPGPPSL AVAPEPCPQP LRSPSLDNPT PFPNLGPSEN PLKRLLVPGE 
       210        220        230        240        250
EWEFEVTAFY RGRQVFQQTI SCPEGLRLVG SEVGDRTLPG WPVTLPDPGM 
       260        270        280        290        300
SLTDRGVMSY VRHVLSCLGG GLALWRAGQW LWAQRLGHCH TYWAVSEELL 
       310        320        330        340        350
PNSGHGPDGE VPKDKEGGVF DLGPFIVDLI TFTEGSGRSP RYALWFCVGE 
       360        370        380        390        400
SWPQDQPWTK RLVMVKVVPT CLRALVEMAR VGGASSLENT VDLHISNSHP 
       410        420 
LSLTSDQYKA YLQDLVEGMD FQGPGES
Length:427
Mass (Da):47,219
Last modified:November 1, 1997 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iF536676FA78B0110
GO
Isoform 2 (identifier: Q14653-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     201-327: Missing.

Show »
Length:300
Mass (Da):33,170
Checksum:i1195BFBBA65C2DCE
GO
Isoform 3 (identifier: Q14653-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-146: Missing.
     201-327: Missing.

Show »
Length:154
Mass (Da):16,730
Checksum:i7A327E1716F74231
GO
Isoform 4 (identifier: Q14653-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     328-427: DLITFTEGSG...GMDFQGPGES → GSWAPRSDYL...NPPVPHHLNQ

Show »
Length:452
Mass (Da):49,134
Checksum:i96B059A029751B64
GO
Isoform 5 (identifier: Q14653-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     56-104: AWAEATGAYV...DRSKDPHDPH → ELGTFPSQTP...SQIRDPQAWL
     105-427: Missing.

Show »
Length:104
Mass (Da):12,330
Checksum:i7DA489EFD316732D
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 13 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
M0R205M0R205_HUMAN
Interferon regulatory factor 3
IRF3
287Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
M0QZB7M0QZB7_HUMAN
Interferon regulatory factor 3
IRF3
408Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
M0R3C2M0R3C2_HUMAN
Interferon regulatory factor 3
IRF3
264Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
M0QYX3M0QYX3_HUMAN
Interferon regulatory factor 3
IRF3
190Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
M0R3E9M0R3E9_HUMAN
Interferon regulatory factor 3
IRF3
153Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
M0QZB8M0QZB8_HUMAN
Interferon regulatory factor 3
IRF3
329Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
M0QYT9M0QYT9_HUMAN
Interferon regulatory factor 3
IRF3
281Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
M0QXC8M0QXC8_HUMAN
Interferon regulatory factor 3
IRF3
119Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
M0R0R8M0R0R8_HUMAN
Interferon regulatory factor 3
IRF3
84Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
M0R0X9M0R0X9_HUMAN
Interferon regulatory factor 3
IRF3
105Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
There are more potential isoformsShow all

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti196L → F in BAG37040 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_08406949Missing Decreased IFNB induction upon Sendai virus infection. 1 Publication1
Natural variantiVAR_01190196R → Q. Corresponds to variant dbSNP:rs968457EnsemblClinVar.1
Natural variantiVAR_049643107Y → F. Corresponds to variant dbSNP:rs34745118Ensembl.1
Natural variantiVAR_084070145 – 200Missing Decreased IFNB induction upon Sendai virus infection. 1 PublicationAdd BLAST56
Natural variantiVAR_084071146N → K Decreased IFNB induction upon Sendai virus infection. 1 Publication1
Natural variantiVAR_084072227R → Q No effect on IFNB induction upon Sendai virus infection. 1 Publication1
Natural variantiVAR_075805285R → Q in IIAE7; loss of viral infection-induced phosphorylation at S-386; loss of viral infection-induced homodimerization; loss of viral infection-induced transcription factor activity; unable to activate interferon transcription in response to viral infection; decreased IFNB induction upon Sendai virus infection. 2 PublicationsCorresponds to variant dbSNP:rs750526659EnsemblClinVar.1
Natural variantiVAR_011902377E → K. Corresponds to variant dbSNP:rs1049486Ensembl.1
Natural variantiVAR_084073401L → V No effect on IFNB induction upon Sendai virus infection. 1 Publication1
Natural variantiVAR_011903427S → T2 PublicationsCorresponds to variant dbSNP:rs7251Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_0469111 – 146Missing in isoform 3. 1 PublicationAdd BLAST146
Alternative sequenceiVSP_04769056 – 104AWAEA…PHDPH → ELGTFPSQTPLRTPMVEAVL LIPRKTFWMSYWVTWCWPHS QIRDPQAWL in isoform 5. 1 PublicationAdd BLAST49
Alternative sequenceiVSP_047691105 – 427Missing in isoform 5. 1 PublicationAdd BLAST323
Alternative sequenceiVSP_043319201 – 327Missing in isoform 2 and isoform 3. 1 PublicationAdd BLAST127
Alternative sequenceiVSP_046912328 – 427DLITF…GPGES → GSWAPRSDYLHGRKRTLTTL CPLVLCGGVMAPGPAVDQEA RDGQGCAHVPQGLGRNGPGR GCLLPGEYCGPAHFQQPPTL PHLRPVQGLPAGLGGGHGFP GPWGELSPRSSWCASNPPVP HHLNQ in isoform 4. 1 PublicationAdd BLAST100

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
Z56281 mRNA Translation: CAA91227.1
AB102884 mRNA Translation: BAD89413.1
AB102886 mRNA Translation: BAD89415.1
AB102887 mRNA Translation: BAD89416.1
AK292027 mRNA Translation: BAF84716.1
AK314421 mRNA Translation: BAG37040.1
AC011495 Genomic DNA No translation available.
CH471177 Genomic DNA Translation: EAW52510.1
BC000660 mRNA Translation: AAH00660.1
BC071721 mRNA Translation: AAH71721.1
U86636 Genomic DNA Translation: AAC68818.1

The Consensus CDS (CCDS) project

More...CCDSi		CCDS12775.1 [Q14653-1]
CCDS56099.1 [Q14653-2]
CCDS59407.1 [Q14653-3]
CCDS59409.1 [Q14653-4]

NCBI Reference Sequences

More...RefSeqi		NP_001184051.1, NM_001197122.1 [Q14653-4]
NP_001184052.1, NM_001197123.1
NP_001184053.1, NM_001197124.1 [Q14653-2]
NP_001184054.1, NM_001197125.1
NP_001184055.1, NM_001197126.1
NP_001184056.1, NM_001197127.1 [Q14653-3]
NP_001184057.1, NM_001197128.1 [Q14653-3]
NP_001562.1, NM_001571.5 [Q14653-1]
XP_006723260.1, XM_006723197.1 [Q14653-4]
XP_006723261.1, XM_006723198.1 [Q14653-4]
XP_016882255.1, XM_017026766.1 [Q14653-1]
XP_016882256.1, XM_017026767.1 [Q14653-1]

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...Ensembli		ENST00000309877.11; ENSP00000310127.6; ENSG00000126456.16
ENST00000377139.8; ENSP00000366344.3; ENSG00000126456.16
ENST00000442265.2; ENSP00000400378.2; ENSG00000126456.16 [Q14653-5]
ENST00000596765.5; ENSP00000470512.1; ENSG00000126456.16 [Q14653-3]
ENST00000597198.5; ENSP00000469113.1; ENSG00000126456.16
ENST00000599223.5; ENSP00000471358.1; ENSG00000126456.16 [Q14653-2]
ENST00000600022.5; ENSP00000472700.1; ENSG00000126456.16 [Q14653-3]
ENST00000601291.5; ENSP00000471896.1; ENSG00000126456.16 [Q14653-4]

Database of genes from NCBI RefSeq genomes

More...GeneIDi		3661

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		hsa:3661

Matched Annotation from NCBI and EMBL-EBI (MANE) - Phase one

More...MANE-Selecti		ENST00000377139.8; ENSP00000366344.3; NM_001571.6; NP_001562.1

UCSC genome browser

More...UCSCi		uc002pot.3, human [Q14653-1]

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z56281 mRNA Translation: CAA91227.1
AB102884 mRNA Translation: BAD89413.1
AB102886 mRNA Translation: BAD89415.1
AB102887 mRNA Translation: BAD89416.1
AK292027 mRNA Translation: BAF84716.1
AK314421 mRNA Translation: BAG37040.1
AC011495 Genomic DNA No translation available.
CH471177 Genomic DNA Translation: EAW52510.1
BC000660 mRNA Translation: AAH00660.1
BC071721 mRNA Translation: AAH71721.1
U86636 Genomic DNA Translation: AAC68818.1
CCDSiCCDS12775.1 [Q14653-1]
CCDS56099.1 [Q14653-2]
CCDS59407.1 [Q14653-3]
CCDS59409.1 [Q14653-4]
RefSeqiNP_001184051.1, NM_001197122.1 [Q14653-4]
NP_001184052.1, NM_001197123.1
NP_001184053.1, NM_001197124.1 [Q14653-2]
NP_001184054.1, NM_001197125.1
NP_001184055.1, NM_001197126.1
NP_001184056.1, NM_001197127.1 [Q14653-3]
NP_001184057.1, NM_001197128.1 [Q14653-3]
NP_001562.1, NM_001571.5 [Q14653-1]
XP_006723260.1, XM_006723197.1 [Q14653-4]
XP_006723261.1, XM_006723198.1 [Q14653-4]
XP_016882255.1, XM_017026766.1 [Q14653-1]
XP_016882256.1, XM_017026767.1 [Q14653-1]

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1J2FX-ray2.30A/B175-427[»]
1QWTX-ray2.10A/B173-427[»]
1T2KX-ray3.00A/B1-112[»]
1ZOQX-ray2.37A/B196-386[»]
2O61X-ray2.80A9-111[»]
2O6GX-ray3.10E/F/G/H1-123[»]
2PI0X-ray2.31A/B/C/D1-113[»]
3A77X-ray1.80A/B/C/D189-427[»]
3QU6X-ray2.30A/B/C1-113[»]
5JEJX-ray2.00A/B189-427[»]
5JEKX-ray2.40A/B189-427[»]
5JELX-ray1.60A189-427[»]
5JEMX-ray2.50A/B/E/G189-398[»]
5JEOX-ray1.72A189-427[»]
5JERX-ray2.91A/C/E/G189-427[»]
6SIVX-ray1.75A137-148[»]
6SJAX-ray1.50A137-148[»]
7JFLX-ray1.68A/B189-398[»]
AlphaFoldDBiQ14653
SMRiQ14653
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi109869, 123 interactors
CORUMiQ14653
DIPiDIP-41448N
ELMiQ14653
IntActiQ14653, 71 interactors
MINTiQ14653
STRINGi9606.ENSP00000471896

Chemistry databases

BindingDBiQ14653
ChEMBLiCHEMBL4523293

PTM databases

iPTMnetiQ14653
PhosphoSitePlusiQ14653

Genetic variation databases

BioMutaiIRF3
DMDMi2497442

Proteomic databases

EPDiQ14653
jPOSTiQ14653
MassIVEiQ14653
MaxQBiQ14653
PaxDbiQ14653
PeptideAtlasiQ14653
PRIDEiQ14653
ProteomicsDBi60091 [Q14653-1]
60092 [Q14653-2]
62791

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...Antibodypediai		1283, 1371 antibodies from 46 providers

The DNASU plasmid repository

More...DNASUi		3661

Genome annotation databases

EnsembliENST00000309877.11; ENSP00000310127.6; ENSG00000126456.16
ENST00000377139.8; ENSP00000366344.3; ENSG00000126456.16
ENST00000442265.2; ENSP00000400378.2; ENSG00000126456.16 [Q14653-5]
ENST00000596765.5; ENSP00000470512.1; ENSG00000126456.16 [Q14653-3]
ENST00000597198.5; ENSP00000469113.1; ENSG00000126456.16
ENST00000599223.5; ENSP00000471358.1; ENSG00000126456.16 [Q14653-2]
ENST00000600022.5; ENSP00000472700.1; ENSG00000126456.16 [Q14653-3]
ENST00000601291.5; ENSP00000471896.1; ENSG00000126456.16 [Q14653-4]
GeneIDi3661
KEGGihsa:3661
MANE-SelectiENST00000377139.8; ENSP00000366344.3; NM_001571.6; NP_001562.1
UCSCiuc002pot.3, human [Q14653-1]

Organism-specific databases

Comparative Toxicogenomics Database

More...CTDi		3661
DisGeNETi3661

GeneCards: human genes, protein and diseases

More...GeneCardsi		IRF3
HGNCiHGNC:6118, IRF3
HPAiENSG00000126456, Low tissue specificity
MalaCardsiIRF3
MIMi603734, gene
616532, phenotype
neXtProtiNX_Q14653
OpenTargetsiENSG00000126456
PharmGKBiPA29917
VEuPathDBiHostDB:ENSG00000126456

GenAtlas: human gene database

More...GenAtlasi		Search...

Phylogenomic databases

eggNOGiENOG502QTRR, Eukaryota
GeneTreeiENSGT00940000160569
HOGENOMiCLU_031544_2_0_1
InParanoidiQ14653
OMAiCHTYWAM
OrthoDBi648909at2759
PhylomeDBiQ14653
TreeFamiTF328512

Enzyme and pathway databases

PathwayCommonsiQ14653
ReactomeiR-HSA-1169408, ISG15 antiviral mechanism
R-HSA-1606341, IRF3 mediated activation of type 1 IFN
R-HSA-168928, DDX58/IFIH1-mediated induction of interferon-alpha/beta
R-HSA-3134973, LRR FLII-interacting protein 1 (LRRFIP1) activates type I IFN production
R-HSA-3134975, Regulation of innate immune responses to cytosolic DNA
R-HSA-3270619, IRF3-mediated induction of type I IFN
R-HSA-877300, Interferon gamma signaling
R-HSA-9013973, TICAM1-dependent activation of IRF3/IRF7
R-HSA-909733, Interferon alpha/beta signaling
R-HSA-918233, TRAF3-dependent IRF activation pathway
R-HSA-933541, TRAF6 mediated IRF7 activation
R-HSA-936440, Negative regulators of DDX58/IFIH1 signaling
R-HSA-936964, Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon
SignaLinkiQ14653
SIGNORiQ14653

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...BioGRID-ORCSi		3661, 14 hits in 1110 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...ChiTaRSi		IRF3, human
EvolutionaryTraceiQ14653

The Gene Wiki collection of pages on human genes and proteins

More...GeneWikii		IRF3

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...GenomeRNAii		3661
PharosiQ14653, Tbio

Protein Ontology

More...PROi		PR:Q14653
RNActiQ14653, protein

The Stanford Online Universal Resource for Clones and ESTs

More...SOURCEi		Search...

Gene expression databases

BgeeiENSG00000126456, Expressed in granulocyte and 222 other tissues
ExpressionAtlasiQ14653, baseline and differential
GenevisibleiQ14653, HS

Family and domain databases

CDDicd00103, IRF, 1 hit
DisProtiDP01614
Gene3Di1.10.10.10, 1 hit
2.60.200.10, 1 hit
IDEALiIID00079
InterProiView protein in InterPro
IPR019817, Interferon_reg_fac_CS
IPR001346, Interferon_reg_fact_DNA-bd_dom
IPR019471, Interferon_reg_factor-3
IPR017855, SMAD-like_dom_sf
IPR008984, SMAD_FHA_dom_sf
IPR036388, WH-like_DNA-bd_sf
IPR036390, WH_DNA-bd_sf
PANTHERiPTHR11949, PTHR11949, 1 hit
PfamiView protein in Pfam
PF00605, IRF, 1 hit
PF10401, IRF-3, 1 hit
PRINTSiPR00267, INTFRNREGFCT
SMARTiView protein in SMART
SM00348, IRF, 1 hit
SM01243, IRF-3, 1 hit
SUPFAMiSSF46785, SSF46785, 1 hit
SSF49879, SSF49879, 1 hit
PROSITEiView protein in PROSITE
PS00601, IRF_1, 1 hit
PS51507, IRF_2, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiIRF3_HUMAN
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q14653
Secondary accession number(s): A8K7L2
, B2RAZ3, Q5FBY1, Q5FBY2, Q5FBY4, Q7Z5G6
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 25, 2022
This is version 207 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with genetic variants
    List of human entries with genetic variants
  3. Human variants curated from literature reports
    Index of human variants curated from literature reports
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
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