UniProtKB - Q14596 (NBR1_HUMAN)
Next to BRCA1 gene 1 protein
NBR1
Functioni
Acts probably as a receptor for selective autophagosomal degradation of ubiquitinated targets.
1 PublicationCaution
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 217 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 220 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 231 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 234 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 240 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 243 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 250 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 254 | Zinc 2PROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 212 – 264 | ZZ-typePROSITE-ProRule annotationAdd BLAST | 53 |
GO - Molecular functioni
- mitogen-activated protein kinase binding Source: BHF-UCL
- ubiquitin binding Source: UniProtKB
- zinc ion binding Source: InterPro
GO - Biological processi
- macroautophagy Source: UniProtKB
- negative regulation of osteoblast differentiation Source: BHF-UCL
- regulation of bone mineralization Source: BHF-UCL
- regulation of stress-activated MAPK cascade Source: BHF-UCL
Keywordsi
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q14596 |
Reactomei | R-HSA-9664873, Pexophagy |
SignaLinki | Q14596 |
SIGNORi | Q14596 |
Names & Taxonomyi
Protein namesi | Recommended name: Next to BRCA1 gene 1 proteinAlternative name(s): Cell migration-inducing gene 19 protein Membrane component chromosome 17 surface marker 2 Neighbor of BRCA1 gene 1 protein Protein 1A1-3B |
Gene namesi | Name:NBR1 Synonyms:1A13B, KIAA0049, M17S2 ORF Names:MIG19 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:6746, NBR1 |
MIMi | 166945, gene |
neXtProti | NX_Q14596 |
VEuPathDBi | HostDB:ENSG00000188554 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Lysosome
- Lysosome 1 Publication
Other locations
- autophagosome 1 Publication
- M line By similarity
Note: In cardiac muscles localizes to the sarcomeric M line (By similarity). Is targeted to lysosomes for degradation (PubMed:19250911).By similarity1 Publication
Cytosol
- cytosol Source: UniProtKB
Endosome
- late endosome Source: UniProtKB
Lysosome
- lysosome Source: UniProtKB-SubCell
Mitochondrion
- mitochondrial intermembrane space Source: Ensembl
Nucleus
- nuclear body Source: HPA
- nucleoplasm Source: HPA
Peroxisome
- peroxisomal membrane Source: Reactome
Other locations
- autophagosome Source: UniProtKB-SubCell
- intracellular membrane-bounded organelle Source: HPA
- M band Source: UniProtKB-SubCell
- membrane Source: UniProtKB
- phagophore assembly site Source: GO_Central
- receptor complex Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Cytoplasmic vesicle, LysosomePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 12 | K → A: No effect on interaction with SQSTM1. 1 Publication | 1 | |
Mutagenesisi | 50 | D → R: Loss of interaction with SQSTM1. 2 Publications | 1 | |
Mutagenesisi | 732 | Y → A: Loss of interaction ATG8 family proteins. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 4077 |
OpenTargetsi | ENSG00000188554 |
PharmGKBi | PA30510 |
Miscellaneous databases
Pharosi | Q14596, Tbio |
Genetic variation databases
BioMutai | NBR1 |
DMDMi | 296439290 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000096746 | 1 – 966 | Next to BRCA1 gene 1 proteinAdd BLAST | 966 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 116 | PhosphoserineCombined sources | 1 | |
Modified residuei | 590 | PhosphoserineBy similarity | 1 | |
Modified residuei | 596 | PhosphoserineBy similarity | 1 | |
Modified residuei | 625 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q14596 |
jPOSTi | Q14596 |
MassIVEi | Q14596 |
MaxQBi | Q14596 |
PaxDbi | Q14596 |
PeptideAtlasi | Q14596 |
PRIDEi | Q14596 |
ProteomicsDBi | 60070 [Q14596-1] 60071 [Q14596-2] |
PTM databases
GlyConnecti | 363, 2 O-Linked glycans |
GlyGeni | Q14596, 1 site, 4 O-linked glycans (1 site) |
iPTMneti | Q14596 |
PhosphoSitePlusi | Q14596 |
Expressioni
Gene expression databases
Bgeei | ENSG00000188554, Expressed in testis and 252 other tissues |
ExpressionAtlasi | Q14596, baseline and differential |
Genevisiblei | Q14596, HS |
Organism-specific databases
HPAi | ENSG00000188554, Low tissue specificity |
Interactioni
Subunit structurei
Homooligomer and heterooligomer.
Interacts with TRIM55 (By similarity).
Interacts with SQSTM1, titin/TTN, RNF29, USP8, SQSTM1, MAP1LC3A, MAP1LC3B, MAP1LC3C, GABARAP, GABARAPL1 and GABARAPL2. Binds to ubiquitin and ubiquitinated proteins.
By similarity4 PublicationsBinary interactionsi
Q14596
Isoform 2 [Q14596-2]
With | #Exp. | IntAct |
---|---|---|
AXIN1 [A0A0S2Z4M1] | 3 | EBI-11081753,EBI-16429430 |
METAP1 [P53582] | 3 | EBI-11081753,EBI-1051435 |
SYT4 [Q9H2B2] | 3 | EBI-11081753,EBI-751132 |
GO - Molecular functioni
- mitogen-activated protein kinase binding Source: BHF-UCL
- ubiquitin binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 110253, 146 interactors |
CORUMi | Q14596 |
IntActi | Q14596, 45 interactors |
MINTi | Q14596 |
STRINGi | 9606.ENSP00000411250 |
Miscellaneous databases
RNActi | Q14596, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q14596 |
BMRBi | Q14596 |
SMRi | Q14596 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q14596 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 4 – 85 | PB1PROSITE-ProRule annotationAdd BLAST | 82 | |
Domaini | 913 – 957 | UBAPROSITE-ProRule annotationAdd BLAST | 45 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 542 – 636 | ATG8 family protein-bindingAdd BLAST | 95 | |
Regioni | 699 – 728 | DisorderedSequence analysisAdd BLAST | 30 | |
Regioni | 727 – 738 | ATG8 family protein-bindingAdd BLAST | 12 | |
Regioni | 750 – 792 | DisorderedSequence analysisAdd BLAST | 43 | |
Regioni | 848 – 879 | DisorderedSequence analysisAdd BLAST | 32 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 699 – 719 | Acidic residuesSequence analysisAdd BLAST | 21 |
Domaini
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 212 – 264 | ZZ-typePROSITE-ProRule annotationAdd BLAST | 53 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG4351, Eukaryota KOG4582, Eukaryota |
GeneTreei | ENSGT00390000016335 |
HOGENOMi | CLU_014175_0_0_1 |
InParanoidi | Q14596 |
OMAi | AMMADGI |
PhylomeDBi | Q14596 |
TreeFami | TF328428 |
Family and domain databases
CDDi | cd14947, NBR1_like, 1 hit cd06396, PB1_NBR1, 1 hit |
Gene3Di | 2.60.40.10, 1 hit 3.30.60.90, 1 hit |
InterProi | View protein in InterPro IPR013783, Ig-like_fold IPR032350, N_BRCA1_central IPR033513, NBR1 IPR000270, PB1_dom IPR034852, PB1_NBR1 IPR015940, UBA IPR009060, UBA-like_sf IPR000433, Znf_ZZ IPR043145, Znf_ZZ_sf |
PANTHERi | PTHR20930:SF2, PTHR20930:SF2, 1 hit |
Pfami | View protein in Pfam PF16158, N_BRCA1_IG, 1 hit PF00564, PB1, 1 hit PF00569, ZZ, 1 hit |
SMARTi | View protein in SMART SM00666, PB1, 1 hit SM00291, ZnF_ZZ, 1 hit |
SUPFAMi | SSF46934, SSF46934, 1 hit |
PROSITEi | View protein in PROSITE PS51745, PB1, 1 hit PS50030, UBA, 1 hit PS01357, ZF_ZZ_1, 1 hit PS50135, ZF_ZZ_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEPQVTLNVT FKNEIQSFLV SDPENTTWAD IEAMVKVSFD LNTIQIKYLD
60 70 80 90 100
EENEEVSINS QGEYEEALKM AVKQGNQLQM QVHEGHHVVD EAPPPVVGAK
110 120 130 140 150
RLAARAGKKP LAHYSSLVRV LGSDMKTPED PAVQSFPLVP CDTDQPQDKP
160 170 180 190 200
PDWFTSYLET FREQVVNETV EKLEQKLHEK LVLQNPSLGS CPSEVSMPTS
210 220 230 240 250
EETLFLPENQ FSWHIACNNC QRRIVGVRYQ CSLCPSYNIC EDCEAGPYGH
260 270 280 290 300
DTNHVLLKLR RPVVGSSEPF CHSKYSTPRL PAALEQVRLQ KQVDKNFLKA
310 320 330 340 350
EKQRLRAEKK QRKAEVKELK KQLKLHRKIH LWNSIHGLQS PKSPLGRPES
360 370 380 390 400
LLQSNTLMLP LQPCTSVMPM LSAAFVDENL PDGTHLQPGT KFIKHWRMKN
410 420 430 440 450
TGNVKWSADT KLKFMWGNLT LASTEKKDVL VPCLKAGHVG VVSVEFIAPA
460 470 480 490 500
LEGTYTSHWR LSHKGQQFGP RVWCSIIVDP FPSEESPDNI EKGMISSSKT
510 520 530 540 550
DDLTCQQEET FLLAKEERQL GEVTEQTEGT AACIPQKAKN VASERELYIP
560 570 580 590 600
SVDLLTAQDL LSFELLDINI VQELERVPHN TPVDVTPCMS PLPHDSPLIE
610 620 630 640 650
KPGLGQIEEE NEGAGFKALP DSMVSVKRKA ENIASVEEAE EDLSGTQFVC
660 670 680 690 700
ETVIRSLTLD AAPDHNPPCR QKSLQMTFAL PEGPLGNEKE EIIHIAEEEA
710 720 730 740 750
VMEEEEDEED EEEEDELKDE VQSQSSASSE DYIIILPECF DTSRPLGDSM
760 770 780 790 800
YSSALSQPGL ERGAEGKPGV EAGQEPAEAG ERLPGGENQP QEHSISDILT
810 820 830 840 850
TSQTLETVPL IPEVVELPPS LPRSSPCVHH HGSPGVDLPV TIPEVSSVPD
860 870 880 890 900
QIRGEPRGSS GLVNSRQKSY DHSRHHHGSS IAGGLVKGAL SVAASAYKAL
910 920 930 940 950
FAGPPVTAQP IISEDQTAAL MAHLFEMGFC DRQLNLRLLK KHNYNILQVV
960
TELLQLNNND WYSQRY
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketB7Z5R6 | B7Z5R6_HUMAN | Next to BRCA1 gene 1 protein | NBR1 | 837 | Annotation score: | ||
A0A0C4DGP6 | A0A0C4DGP6_HUMAN | Next to BRCA1 gene 1 protein | NBR1 | 42 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 746 – 770 | LGDSM…GKPGV → WGILCTALRSHSQAWSEVLK ASLGF in CAA54274 (PubMed:8069304).CuratedAdd BLAST | 25 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_016106 | 923 | H → R3 PublicationsCorresponds to variant dbSNP:rs8482Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_004314 | 910 – 966 | PIISE…YSQRY → GLWGLLSFLHLAKKCFFLKA PSEAFSWF in isoform 2. 1 PublicationAdd BLAST | 57 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X76952 mRNA Translation: CAA54274.1 D30756 mRNA Translation: BAA06417.2 Different initiation. AY450308 mRNA Translation: AAS15047.1 AC060780 Genomic DNA No translation available. AC109326 Genomic DNA No translation available. CH471152 Genomic DNA Translation: EAW60946.1 BC009808 mRNA Translation: AAH09808.1 AF227189 mRNA Translation: AAF74119.1 U25764 Genomic DNA Translation: AAA93228.1 |
CCDSi | CCDS45694.1 [Q14596-1] CCDS77037.1 [Q14596-2] |
RefSeqi | NP_001278500.1, NM_001291571.1 [Q14596-2] NP_001278501.1, NM_001291572.1 NP_005890.2, NM_005899.4 [Q14596-1] NP_114068.1, NM_031862.3 [Q14596-1] XP_011523115.1, XM_011524813.1 [Q14596-1] XP_016880131.1, XM_017024642.1 [Q14596-1] |
Genome annotation databases
Ensembli | ENST00000341165.10; ENSP00000343479.5; ENSG00000188554.15 ENST00000589872.1; ENSP00000467816.1; ENSG00000188554.15 [Q14596-2] ENST00000590996.6; ENSP00000466667.1; ENSG00000188554.15 |
GeneIDi | 4077 |
KEGGi | hsa:4077 |
MANE-Selecti | ENST00000590996.6; ENSP00000466667.1; NM_005899.5; NP_005890.2 |
UCSCi | uc010czd.4, human [Q14596-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X76952 mRNA Translation: CAA54274.1 D30756 mRNA Translation: BAA06417.2 Different initiation. AY450308 mRNA Translation: AAS15047.1 AC060780 Genomic DNA No translation available. AC109326 Genomic DNA No translation available. CH471152 Genomic DNA Translation: EAW60946.1 BC009808 mRNA Translation: AAH09808.1 AF227189 mRNA Translation: AAF74119.1 U25764 Genomic DNA Translation: AAA93228.1 |
CCDSi | CCDS45694.1 [Q14596-1] CCDS77037.1 [Q14596-2] |
RefSeqi | NP_001278500.1, NM_001291571.1 [Q14596-2] NP_001278501.1, NM_001291572.1 NP_005890.2, NM_005899.4 [Q14596-1] NP_114068.1, NM_031862.3 [Q14596-1] XP_011523115.1, XM_011524813.1 [Q14596-1] XP_016880131.1, XM_017024642.1 [Q14596-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1WJ6 | NMR | - | A | 1-85 | [»] | |
2BKF | X-ray | 1.56 | A | 1-85 | [»] | |
2CP8 | NMR | - | A | 916-956 | [»] | |
2G4S | X-ray | 2.15 | A | 1-85 | [»] | |
2L8J | NMR | - | B | 726-738 | [»] | |
2MGW | NMR | - | A | 913-959 | [»] | |
2MJ5 | NMR | - | B | 913-959 | [»] | |
4OLE | X-ray | 2.52 | A/B/C/D | 365-485 | [»] | |
AlphaFoldDBi | Q14596 | |||||
BMRBi | Q14596 | |||||
SMRi | Q14596 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 110253, 146 interactors |
CORUMi | Q14596 |
IntActi | Q14596, 45 interactors |
MINTi | Q14596 |
STRINGi | 9606.ENSP00000411250 |
PTM databases
GlyConnecti | 363, 2 O-Linked glycans |
GlyGeni | Q14596, 1 site, 4 O-linked glycans (1 site) |
iPTMneti | Q14596 |
PhosphoSitePlusi | Q14596 |
Genetic variation databases
BioMutai | NBR1 |
DMDMi | 296439290 |
Proteomic databases
EPDi | Q14596 |
jPOSTi | Q14596 |
MassIVEi | Q14596 |
MaxQBi | Q14596 |
PaxDbi | Q14596 |
PeptideAtlasi | Q14596 |
PRIDEi | Q14596 |
ProteomicsDBi | 60070 [Q14596-1] 60071 [Q14596-2] |
Protocols and materials databases
Antibodypediai | 8116, 298 antibodies from 32 providers |
DNASUi | 4077 |
Genome annotation databases
Ensembli | ENST00000341165.10; ENSP00000343479.5; ENSG00000188554.15 ENST00000589872.1; ENSP00000467816.1; ENSG00000188554.15 [Q14596-2] ENST00000590996.6; ENSP00000466667.1; ENSG00000188554.15 |
GeneIDi | 4077 |
KEGGi | hsa:4077 |
MANE-Selecti | ENST00000590996.6; ENSP00000466667.1; NM_005899.5; NP_005890.2 |
UCSCi | uc010czd.4, human [Q14596-1] |
Organism-specific databases
CTDi | 4077 |
DisGeNETi | 4077 |
GeneCardsi | NBR1 |
HGNCi | HGNC:6746, NBR1 |
HPAi | ENSG00000188554, Low tissue specificity |
MIMi | 166945, gene |
neXtProti | NX_Q14596 |
OpenTargetsi | ENSG00000188554 |
PharmGKBi | PA30510 |
VEuPathDBi | HostDB:ENSG00000188554 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4351, Eukaryota KOG4582, Eukaryota |
GeneTreei | ENSGT00390000016335 |
HOGENOMi | CLU_014175_0_0_1 |
InParanoidi | Q14596 |
OMAi | AMMADGI |
PhylomeDBi | Q14596 |
TreeFami | TF328428 |
Enzyme and pathway databases
PathwayCommonsi | Q14596 |
Reactomei | R-HSA-9664873, Pexophagy |
SignaLinki | Q14596 |
SIGNORi | Q14596 |
Miscellaneous databases
BioGRID-ORCSi | 4077, 23 hits in 1079 CRISPR screens |
ChiTaRSi | NBR1, human |
EvolutionaryTracei | Q14596 |
GeneWikii | NBR1 |
GenomeRNAii | 4077 |
Pharosi | Q14596, Tbio |
PROi | PR:Q14596 |
RNActi | Q14596, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000188554, Expressed in testis and 252 other tissues |
ExpressionAtlasi | Q14596, baseline and differential |
Genevisiblei | Q14596, HS |
Family and domain databases
CDDi | cd14947, NBR1_like, 1 hit cd06396, PB1_NBR1, 1 hit |
Gene3Di | 2.60.40.10, 1 hit 3.30.60.90, 1 hit |
InterProi | View protein in InterPro IPR013783, Ig-like_fold IPR032350, N_BRCA1_central IPR033513, NBR1 IPR000270, PB1_dom IPR034852, PB1_NBR1 IPR015940, UBA IPR009060, UBA-like_sf IPR000433, Znf_ZZ IPR043145, Znf_ZZ_sf |
PANTHERi | PTHR20930:SF2, PTHR20930:SF2, 1 hit |
Pfami | View protein in Pfam PF16158, N_BRCA1_IG, 1 hit PF00564, PB1, 1 hit PF00569, ZZ, 1 hit |
SMARTi | View protein in SMART SM00666, PB1, 1 hit SM00291, ZnF_ZZ, 1 hit |
SUPFAMi | SSF46934, SSF46934, 1 hit |
PROSITEi | View protein in PROSITE PS51745, PB1, 1 hit PS50030, UBA, 1 hit PS01357, ZF_ZZ_1, 1 hit PS50135, ZF_ZZ_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NBR1_HUMAN | |
Accessioni | Q14596Primary (citable) accession number: Q14596 Secondary accession number(s): Q13173 Q9NRF7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | May 18, 2010 | |
Last modified: | May 25, 2022 | |
This is version 209 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 17
Human chromosome 17: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references