UniProtKB - Q14511 (CASL_HUMAN)
Enhancer of filamentation 1
NEDD9
Functioni
GO - Molecular functioni
- protein tyrosine kinase binding Source: UniProtKB
GO - Biological processi
- actin filament bundle assembly Source: UniProtKB
- actin filament reorganization Source: GO_Central
- activation of GTPase activity Source: Ensembl
- cell adhesion Source: ProtInc
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB-KW
- cell migration Source: GO_Central
- cytoskeleton organization Source: UniProtKB
- integrin-mediated signaling pathway Source: UniProtKB
- positive regulation of cell migration Source: UniProtKB
- positive regulation of protein tyrosine kinase activity Source: UniProtKB
- positive regulation of substrate adhesion-dependent cell spreading Source: UniProtKB
- regulation of growth Source: UniProtKB-KW
- signal transduction Source: ProtInc
- transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central
Keywordsi
Biological process | Cell adhesion, Cell cycle, Cell division, Growth regulation, Mitosis |
Enzyme and pathway databases
PathwayCommonsi | Q14511 |
SignaLinki | Q14511 |
SIGNORi | Q14511 |
Names & Taxonomyi
Protein namesi | Recommended name: Enhancer of filamentation 1Short name: hEF1 Alternative name(s): CRK-associated substrate-related protein Short name: CAS-L Short name: CasL Cas scaffolding protein family member 2 Neural precursor cell expressed developmentally down-regulated protein 9 Short name: NEDD-9 Renal carcinoma antigen NY-REN-12 p105 Cleaved into the following chain: |
Gene namesi | Name:NEDD9 Synonyms:CASL, CASS2 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:7733, NEDD9 |
MIMi | 602265, gene |
neXtProti | NX_Q14511 |
VEuPathDBi | HostDB:ENSG00000111859.16 |
Subcellular locationi
Nucleus
Cytoplasm and Cytosol
Golgi apparatus
Other locations
Note: Localizes to both the cell nucleus and the cell periphery and is differently localized in fibroblasts and epithelial cells. In fibroblasts is predominantly nuclear and in some cells is present in the Golgi apparatus. In epithelial cells localized predominantly in the cell periphery with particular concentration in lamellipodia but is also found in the nucleus. Isoforms p105 and p115 are predominantly cytoplasmic and associate with focal adhesions while p55 associates with mitotic spindle.
Cytoskeleton
Cytoskeleton
- spindle Source: ProtInc
- spindle pole Source: Ensembl
Cytosol
- cytosol Source: HPA
Golgi apparatus
- Golgi apparatus Source: UniProtKB-SubCell
Nucleus
- nucleoplasm Source: HPA
- nucleus Source: ProtInc
Plasma Membrane
- plasma membrane Source: HPA
Other locations
- cell cortex Source: UniProtKB-SubCell
- cytoplasm Source: GO_Central
- focal adhesion Source: UniProtKB-SubCell
- lamellipodium Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell junction, Cell projection, Cytoplasm, Cytoskeleton, Golgi apparatus, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 4739 |
OpenTargetsi | ENSG00000111859 |
PharmGKBi | PA31538 |
Miscellaneous databases
Pharosi | Q14511, Tbio |
Genetic variation databases
BioMutai | NEDD9 |
DMDMi | 8134360 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000089328 | 1 – 834 | Enhancer of filamentation 1Add BLAST | 834 | |
ChainiPRO_0000296242 | 1 – ? | Enhancer of filamentation 1 p55 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 296 | Phosphoserine1 Publication | 1 | |
Modified residuei | 369 | Phosphoserine1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | Q14511 |
MassIVEi | Q14511 |
MaxQBi | Q14511 |
PaxDbi | Q14511 |
PeptideAtlasi | Q14511 |
PRIDEi | Q14511 |
ProteomicsDBi | 34087 60015 [Q14511-1] 60016 [Q14511-2] |
PTM databases
GlyGeni | Q14511, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q14511 |
PhosphoSitePlusi | Q14511 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSG00000111859, Expressed in metanephric glomerulus and 223 other tissues |
ExpressionAtlasi | Q14511, baseline and differential |
Genevisiblei | Q14511, HS |
Organism-specific databases
HPAi | ENSG00000111859, Tissue enhanced (adipose) |
Interactioni
Subunit structurei
Homodimer. Can heterodimerize with HLH proteins ID2, E12, E47 and also with p130cas. Forms complexes in vivo with related adhesion focal tyrosine kinase (RAFTK), adapter protein CRKL and LYN kinase.
Interacts with MICAL and TXNL4/DIM1.
Interacts with BCAR3 (via Ras-GEF domain) (By similarity).
Interacts with SH2D3C isoform 1 and isoform 2 (By similarity).
By similarity2 PublicationsBinary interactionsi
Hide detailsQ14511
Isoform 2 [Q14511-2]
GO - Molecular functioni
- protein tyrosine kinase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 110816, 74 interactors |
CORUMi | Q14511 |
ELMi | Q14511 |
IntActi | Q14511, 50 interactors |
MINTi | Q14511 |
STRINGi | 9606.ENSP00000368759 |
Miscellaneous databases
RNActi | Q14511, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | Q14511 |
SMRi | Q14511 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 3 – 65 | SH3PROSITE-ProRule annotationAdd BLAST | 63 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 102 – 229 | Interacts strongly with spindle-regulatory protein D1M1Add BLAST | 128 | |
Regioni | 710 – 760 | Divergent helix-loop-helix motifAdd BLAST | 51 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 360 – 363 | Caspase cleavage related site | 4 |
Domaini
Sequence similaritiesi
Keywords - Domaini
SH3 domainPhylogenomic databases
eggNOGi | ENOG502QQHE, Eukaryota |
GeneTreei | ENSGT00950000183008 |
HOGENOMi | CLU_017000_1_0_1 |
InParanoidi | Q14511 |
OMAi | SQMQLLH |
PhylomeDBi | Q14511 |
TreeFami | TF328782 |
Family and domain databases
CDDi | cd12002, SH3_NEDD9, 1 hit |
Gene3Di | 1.20.120.830, 1 hit |
InterProi | View protein in InterPro IPR021901, CAS_C IPR037362, CAS_fam IPR035746, NEDD9_SH3 IPR014928, Serine_rich_dom IPR038319, Serine_rich_sf IPR036028, SH3-like_dom_sf IPR001452, SH3_domain |
PANTHERi | PTHR10654, PTHR10654, 1 hit |
Pfami | View protein in Pfam PF12026, CAS_C, 1 hit PF08824, Serine_rich, 1 hit PF14604, SH3_9, 1 hit |
SMARTi | View protein in SMART SM00326, SH3, 1 hit |
SUPFAMi | SSF50044, SSF50044, 1 hit |
PROSITEi | View protein in PROSITE PS50002, SH3, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MKYKNLMARA LYDNVPECAE ELAFRKGDIL TVIEQNTGGL EGWWLCSLHG
60 70 80 90 100
RQGIVPGNRV KLLIGPMQET ASSHEQPASG LMQQTFGQQK LYQVPNPQAA
110 120 130 140 150
PRDTIYQVPP SYQNQGIYQV PTGHGTQEQE VYQVPPSVQR SIGGTSGPHV
160 170 180 190 200
GKKVITPVRT GHGYVYEYPS RYQKDVYDIP PSHTTQGVYD IPPSSAKGPV
210 220 230 240 250
FSVPVGEIKP QGVYDIPPTK GVYAIPPSAC RDEAGLREKD YDFPPPMRQA
260 270 280 290 300
GRPDLRPEGV YDIPPTCTKP AGKDLHVKYN CDIPGAAEPV ARRHQSLSPN
310 320 330 340 350
HPPPQLGQSV GSQNDAYDVP RGVQFLEPPA ETSEKANPQE RDGVYDVPLH
360 370 380 390 400
NPPDAKGSRD LVDGINRLSF SSTGSTRSNM STSSTSSKES SLSASPAQDK
410 420 430 440 450
RLFLDPDTAI ERLQRLQQAL EMGVSSLMAL VTTDWRCYGY MERHINEIRT
460 470 480 490 500
AVDKVELFLK EYLHFVKGAV ANAACLPELI LHNKMKRELQ RVEDSHQILS
510 520 530 540 550
QTSHDLNECS WSLNILAINK PQNKCDDLDR FVMVAKTVPD DAKQLTTTIN
560 570 580 590 600
TNAEALFRPG PGSLHLKNGP ESIMNSTEYP HGGSQGQLLH PGDHKAQAHN
610 620 630 640 650
KALPPGLSKE QAPDCSSSDG SERSWMDDYD YVHLQGKEEF ERQQKELLEK
660 670 680 690 700
ENIMKQNKMQ LEHHQLSQFQ LLEQEITKPV ENDISKWKPS QSLPTTNSGV
710 720 730 740 750
SAQDRQLLCF YYDQCETHFI SLLNAIDALF SCVSSAQPPR IFVAHSKFVI
760 770 780 790 800
LSAHKLVFIG DTLTRQVTAQ DIRNKVMNSS NQLCEQLKTI VMATKMAALH
810 820 830
YPSTTALQEM VHQVTDLSRN AQLFKRSLLE MATF
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A087WUD2 | A0A087WUD2_HUMAN | Enhancer of filamentation 1 | NEDD9 | 685 | Annotation score: | ||
D6RGD7 | D6RGD7_HUMAN | Enhancer of filamentation 1 | NEDD9 | 29 | Annotation score: | ||
D6RBQ2 | D6RBQ2_HUMAN | Enhancer of filamentation 1 | NEDD9 | 32 | Annotation score: | ||
D6REP7 | D6REP7_HUMAN | Enhancer of filamentation 1 | NEDD9 | 22 | Annotation score: | ||
D6RDV1 | D6RDV1_HUMAN | Enhancer of filamentation 1 | NEDD9 | 43 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 139 | Q → R in BAF85371 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_054082 | 178 | D → N. Corresponds to variant dbSNP:rs11546959Ensembl. | 1 | |
Natural variantiVAR_054083 | 304 | P → L. Corresponds to variant dbSNP:rs34184473Ensembl. | 1 | |
Natural variantiVAR_021857 | 577 | T → M. Corresponds to variant dbSNP:rs3734401Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_044579 | 1 – 4 | MKYK → MWTR in isoform 3. 1 Publication | 4 | |
Alternative sequenceiVSP_042835 | 155 – 174 | ITPVR…SRYQK → FQRDGQVSYFLVRASKQTSL in isoform 2. 1 PublicationAdd BLAST | 20 | |
Alternative sequenceiVSP_042836 | 175 – 834 | Missing in isoform 2. 1 PublicationAdd BLAST | 660 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L43821 mRNA Translation: AAA98770.1 U64317 mRNA Translation: AAB53696.1 AK292682 mRNA Translation: BAF85371.1 AL022098 Genomic DNA No translation available. AL136139 Genomic DNA No translation available. AL139807 Genomic DNA No translation available. AL512382 Genomic DNA No translation available. CH471087 Genomic DNA Translation: EAW55299.1 CH471087 Genomic DNA Translation: EAW55301.1 BC020686 mRNA Translation: AAH20686.1 BC040207 mRNA Translation: AAH40207.1 |
CCDSi | CCDS34340.1 [Q14511-2] CCDS4520.1 [Q14511-1] CCDS47373.1 [Q14511-3] |
RefSeqi | NP_001135865.1, NM_001142393.1 [Q14511-3] NP_001257962.1, NM_001271033.1 NP_006394.1, NM_006403.3 [Q14511-1] NP_892011.2, NM_182966.3 [Q14511-2] |
Genome annotation databases
Ensembli | ENST00000379433; ENSP00000368745; ENSG00000111859 [Q14511-2] ENST00000379446; ENSP00000368759; ENSG00000111859 [Q14511-1] ENST00000504387; ENSP00000422871; ENSG00000111859 [Q14511-3] |
GeneIDi | 4739 |
KEGGi | hsa:4739 |
UCSCi | uc003mzv.2, human [Q14511-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L43821 mRNA Translation: AAA98770.1 U64317 mRNA Translation: AAB53696.1 AK292682 mRNA Translation: BAF85371.1 AL022098 Genomic DNA No translation available. AL136139 Genomic DNA No translation available. AL139807 Genomic DNA No translation available. AL512382 Genomic DNA No translation available. CH471087 Genomic DNA Translation: EAW55299.1 CH471087 Genomic DNA Translation: EAW55301.1 BC020686 mRNA Translation: AAH20686.1 BC040207 mRNA Translation: AAH40207.1 |
CCDSi | CCDS34340.1 [Q14511-2] CCDS4520.1 [Q14511-1] CCDS47373.1 [Q14511-3] |
RefSeqi | NP_001135865.1, NM_001142393.1 [Q14511-3] NP_001257962.1, NM_001271033.1 NP_006394.1, NM_006403.3 [Q14511-1] NP_892011.2, NM_182966.3 [Q14511-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2L81 | NMR | - | A | 399-563 | [»] | |
5X3S | X-ray | 2.90 | C/D | 799-809 | [»] | |
BMRBi | Q14511 | |||||
SMRi | Q14511 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 110816, 74 interactors |
CORUMi | Q14511 |
ELMi | Q14511 |
IntActi | Q14511, 50 interactors |
MINTi | Q14511 |
STRINGi | 9606.ENSP00000368759 |
PTM databases
GlyGeni | Q14511, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q14511 |
PhosphoSitePlusi | Q14511 |
Genetic variation databases
BioMutai | NEDD9 |
DMDMi | 8134360 |
Proteomic databases
jPOSTi | Q14511 |
MassIVEi | Q14511 |
MaxQBi | Q14511 |
PaxDbi | Q14511 |
PeptideAtlasi | Q14511 |
PRIDEi | Q14511 |
ProteomicsDBi | 34087 60015 [Q14511-1] 60016 [Q14511-2] |
Protocols and materials databases
Antibodypediai | 10092, 344 antibodies |
DNASUi | 4739 |
Genome annotation databases
Ensembli | ENST00000379433; ENSP00000368745; ENSG00000111859 [Q14511-2] ENST00000379446; ENSP00000368759; ENSG00000111859 [Q14511-1] ENST00000504387; ENSP00000422871; ENSG00000111859 [Q14511-3] |
GeneIDi | 4739 |
KEGGi | hsa:4739 |
UCSCi | uc003mzv.2, human [Q14511-1] |
Organism-specific databases
CTDi | 4739 |
DisGeNETi | 4739 |
GeneCardsi | NEDD9 |
HGNCi | HGNC:7733, NEDD9 |
HPAi | ENSG00000111859, Tissue enhanced (adipose) |
MIMi | 602265, gene |
neXtProti | NX_Q14511 |
OpenTargetsi | ENSG00000111859 |
PharmGKBi | PA31538 |
VEuPathDBi | HostDB:ENSG00000111859.16 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QQHE, Eukaryota |
GeneTreei | ENSGT00950000183008 |
HOGENOMi | CLU_017000_1_0_1 |
InParanoidi | Q14511 |
OMAi | SQMQLLH |
PhylomeDBi | Q14511 |
TreeFami | TF328782 |
Enzyme and pathway databases
PathwayCommonsi | Q14511 |
SignaLinki | Q14511 |
SIGNORi | Q14511 |
Miscellaneous databases
BioGRID-ORCSi | 4739, 10 hits in 989 CRISPR screens |
ChiTaRSi | NEDD9, human |
GeneWikii | NEDD9 |
GenomeRNAii | 4739 |
Pharosi | Q14511, Tbio |
PROi | PR:Q14511 |
RNActi | Q14511, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000111859, Expressed in metanephric glomerulus and 223 other tissues |
ExpressionAtlasi | Q14511, baseline and differential |
Genevisiblei | Q14511, HS |
Family and domain databases
CDDi | cd12002, SH3_NEDD9, 1 hit |
Gene3Di | 1.20.120.830, 1 hit |
InterProi | View protein in InterPro IPR021901, CAS_C IPR037362, CAS_fam IPR035746, NEDD9_SH3 IPR014928, Serine_rich_dom IPR038319, Serine_rich_sf IPR036028, SH3-like_dom_sf IPR001452, SH3_domain |
PANTHERi | PTHR10654, PTHR10654, 1 hit |
Pfami | View protein in Pfam PF12026, CAS_C, 1 hit PF08824, Serine_rich, 1 hit PF14604, SH3_9, 1 hit |
SMARTi | View protein in SMART SM00326, SH3, 1 hit |
SUPFAMi | SSF50044, SSF50044, 1 hit |
PROSITEi | View protein in PROSITE PS50002, SH3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CASL_HUMAN | |
Accessioni | Q14511Primary (citable) accession number: Q14511 Secondary accession number(s): A8K9G7 Q5XKI0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | November 1, 1996 | |
Last modified: | April 7, 2021 | |
This is version 213 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 6
Human chromosome 6: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families