UniProtKB - Q14435 (GALT3_HUMAN)
Polypeptide N-acetylgalactosaminyltransferase 3
GALNT3
Functioni
Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has activity toward HIV envelope glycoprotein gp120, EA2, Muc2 and Muc5. Probably glycosylates fibronectin in vivo. Glycosylates FGF23. Plays a central role in phosphate homeostasis.
2 PublicationsMiscellaneous
Catalytic activityi
- L-seryl-[protein] + UDP-N-acetyl-α-D-galactosamine = 3-O-[N-acetyl-α-D-galactosaminyl]-L-seryl-[protein] + H+ + UDP1 PublicationEC:2.4.1.411 Publication
- L-threonyl-[protein] + UDP-N-acetyl-α-D-galactosamine = 3-O-[N-acetyl-α-D-galactosaminyl]-L-threonyl-[protein] + H+ + UDP1 PublicationEC:2.4.1.411 Publication
Cofactori
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 225 | SubstrateBy similarity | 1 | |
Binding sitei | 254 | SubstrateBy similarity | 1 | |
Metal bindingi | 277 | ManganeseBy similarity | 1 | |
Metal bindingi | 279 | ManganeseBy similarity | 1 | |
Binding sitei | 387 | SubstrateBy similarity | 1 | |
Metal bindingi | 415 | ManganeseBy similarity | 1 | |
Binding sitei | 418 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- calcium ion binding Source: BHF-UCL
- carbohydrate binding Source: UniProtKB-KW
- manganese ion binding Source: BHF-UCL
- polypeptide N-acetylgalactosaminyltransferase activity Source: BHF-UCL
GO - Biological processi
- carbohydrate metabolic process Source: ProtInc
- fibroblast growth factor receptor signaling pathway Source: Reactome
- O-glycan processing Source: Reactome
- protein O-linked glycosylation Source: GO_Central
- protein O-linked glycosylation via serine Source: BHF-UCL
- protein O-linked glycosylation via threonine Source: UniProtKB
- spermatogenesis Source: Ensembl
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Ligand | Lectin, Manganese, Metal-binding |
Enzyme and pathway databases
BRENDAi | 2.4.1.41, 2681 |
PathwayCommonsi | Q14435 |
Reactomei | R-HSA-190372, FGFR3c ligand binding and activation R-HSA-5083625, Defective GALNT3 causes HFTC R-HSA-913709, O-linked glycosylation of mucins |
SignaLinki | Q14435 |
UniPathwayi | UPA00378 |
Protein family/group databases
CAZyi | CBM13, Carbohydrate-Binding Module Family 13 GT27, Glycosyltransferase Family 27 |
Names & Taxonomyi
Protein namesi | Recommended name: Polypeptide N-acetylgalactosaminyltransferase 3 (EC:2.4.1.41)Alternative name(s): Polypeptide GalNAc transferase 3 Short name: GalNAc-T3 Short name: pp-GaNTase 3 Protein-UDP acetylgalactosaminyltransferase 3 UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3 |
Gene namesi | Name:GALNT3 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:4125, GALNT3 |
MIMi | 601756, gene |
neXtProti | NX_Q14435 |
VEuPathDBi | HostDB:ENSG00000115339 |
Subcellular locationi
Golgi apparatus
- Golgi stack membrane 1 Publication; Single-pass type II membrane protein 1 Publication
Note: Resides preferentially in the trans and medial parts of the Golgi stack.
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Golgi apparatus
- Golgi apparatus Source: HPA
- Golgi cisterna membrane Source: UniProtKB-SubCell
- Golgi membrane Source: Reactome
Other locations
- integral component of membrane Source: ProtInc
- membrane Source: ProtInc
- perinuclear region of cytoplasm Source: BHF-UCL
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 19 | CytoplasmicSequence analysisAdd BLAST | 19 | |
Transmembranei | 20 – 37 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 18 | |
Topological domaini | 38 – 633 | LumenalSequence analysisAdd BLAST | 596 |
Keywords - Cellular componenti
Golgi apparatus, MembranePathology & Biotechi
Involvement in diseasei
Tumoral calcinosis, hyperphosphatemic, familial, 1 (HFTC1)2 Publications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_080832 | 162 – 633 | Missing in HFTC1. 1 PublicationAdd BLAST | 472 |
Keywords - Diseasei
Disease variantOrganism-specific databases
DisGeNETi | 2591 |
GeneReviewsi | GALNT3 |
MalaCardsi | GALNT3 |
MIMi | 211900, phenotype |
OpenTargetsi | ENSG00000115339 |
Orphaneti | 306661, Familial hyperphosphatemic tumoral calcinosis/Hyperphosphatemic hyperostosis syndrome |
PharmGKBi | PA28538 |
Miscellaneous databases
Pharosi | Q14435, Tbio |
Chemistry databases
ChEMBLi | CHEMBL4523291 |
Genetic variation databases
BioMutai | GALNT3 |
DMDMi | 209572629 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000059106 | 1 – 633 | Polypeptide N-acetylgalactosaminyltransferase 3Add BLAST | 633 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 132 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 173 ↔ 410 | PROSITE-ProRule annotation | ||
Glycosylationi | 297 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 401 ↔ 482 | PROSITE-ProRule annotation | ||
Glycosylationi | 484 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 517 ↔ 535 | PROSITE-ProRule annotation | ||
Disulfide bondi | 561 ↔ 574 | PROSITE-ProRule annotation | ||
Disulfide bondi | 605 ↔ 618 | PROSITE-ProRule annotation |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
EPDi | Q14435 |
jPOSTi | Q14435 |
MassIVEi | Q14435 |
MaxQBi | Q14435 |
PaxDbi | Q14435 |
PeptideAtlasi | Q14435 |
PRIDEi | Q14435 |
ProteomicsDBi | 59988 [Q14435-1] 59989 [Q14435-2] |
PTM databases
GlyConnecti | 1620, 3 N-Linked glycans (1 site) |
GlyGeni | Q14435, 3 sites, 3 N-linked glycans (1 site) |
iPTMneti | Q14435 |
PhosphoSitePlusi | Q14435 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000115339, Expressed in colonic mucosa and 189 other tissues |
ExpressionAtlasi | Q14435, baseline and differential |
Genevisiblei | Q14435, HS |
Organism-specific databases
HPAi | ENSG00000115339, Tissue enhanced (stomach) |
Interactioni
Binary interactionsi
Isoform 2 [Q14435-2]
With | #Exp. | IntAct |
---|---|---|
APPBP2 [Q92624] | 3 | EBI-10232904,EBI-743771 |
Protein-protein interaction databases
BioGRIDi | 108863, 25 interactors |
IntActi | Q14435, 6 interactors |
STRINGi | 9606.ENSP00000376465 |
Chemistry databases
BindingDBi | Q14435 |
Miscellaneous databases
RNActi | Q14435, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 504 – 630 | Ricin B-type lectinPROSITE-ProRule annotationAdd BLAST | 127 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 184 – 293 | Catalytic subdomain AAdd BLAST | 110 | |
Regioni | 356 – 418 | Catalytic subdomain BAdd BLAST | 63 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3736, Eukaryota |
GeneTreei | ENSGT00940000156609 |
InParanoidi | Q14435 |
OMAi | NGEHPNL |
OrthoDBi | 606683at2759 |
PhylomeDBi | Q14435 |
TreeFami | TF313267 |
Family and domain databases
CDDi | cd02510, pp-GalNAc-T, 1 hit cd00161, RICIN, 1 hit |
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR045885, GalNAc-T IPR001173, Glyco_trans_2-like IPR029044, Nucleotide-diphossugar_trans IPR035992, Ricin_B-like_lectins IPR000772, Ricin_B_lectin |
Pfami | View protein in Pfam PF00535, Glycos_transf_2, 1 hit PF00652, Ricin_B_lectin, 1 hit |
SMARTi | View protein in SMART SM00458, RICIN, 1 hit |
SUPFAMi | SSF50370, SSF50370, 1 hit SSF53448, SSF53448, 1 hit |
PROSITEi | View protein in PROSITE PS50231, RICIN_B_LECTIN, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAHLKRLVKL HIKRHYHKKF WKLGAVIFFF IIVLVLMQRE VSVQYSKEES
60 70 80 90 100
RMERNMKNKN KMLDLMLEAV NNIKDAMPKM QIGAPVRQNI DAGERPCLQG
110 120 130 140 150
YYTAAELKPV LDRPPQDSNA PGASGKAFKT TNLSVEEQKE KERGEAKHCF
160 170 180 190 200
NAFASDRISL HRDLGPDTRP PECIEQKFKR CPPLPTTSVI IVFHNEAWST
210 220 230 240 250
LLRTVHSVLY SSPAILLKEI ILVDDASVDE YLHDKLDEYV KQFSIVKIVR
260 270 280 290 300
QRERKGLITA RLLGATVATA ETLTFLDAHC ECFYGWLEPL LARIAENYTA
310 320 330 340 350
VVSPDIASID LNTFEFNKPS PYGSNHNRGN FDWSLSFGWE SLPDHEKQRR
360 370 380 390 400
KDETYPIKTP TFAGGLFSIS KEYFEYIGSY DEEMEIWGGE NIEMSFRVWQ
410 420 430 440 450
CGGQLEIMPC SVVGHVFRSK SPHSFPKGTQ VIARNQVRLA EVWMDEYKEI
460 470 480 490 500
FYRRNTDAAK IVKQKAFGDL SKRFEIKHRL QCKNFTWYLN NIYPEVYVPD
510 520 530 540 550
LNPVISGYIK SVGQPLCLDV GENNQGGKPL IMYTCHGLGG NQYFEYSAQH
560 570 580 590 600
EIRHNIQKEL CLHAAQGLVQ LKACTYKGHK TVVTGEQIWE IQKDQLLYNP
610 620 630
FLKMCLSANG EHPSLVSCNP SDPLQKWILS QND
Computationally mapped potential isoform sequencesi
There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC9J2C3 | C9J2C3_HUMAN | Polypeptide N-acetylgalactosaminylt... | GALNT3 | 464 | Annotation score: | ||
E7EUL0 | E7EUL0_HUMAN | Polypeptide N-acetylgalactosaminylt... | GALNT3 | 371 | Annotation score: | ||
C9J388 | C9J388_HUMAN | Polypeptide N-acetylgalactosaminylt... | GALNT3 | 90 | Annotation score: | ||
C9JXX2 | C9JXX2_HUMAN | Polypeptide N-acetylgalactosaminylt... | GALNT3 | 57 | Annotation score: | ||
H7BZS4 | H7BZS4_HUMAN | Polypeptide N-acetylgalactosaminylt... | GALNT3 | 89 | Annotation score: | ||
C9JW45 | C9JW45_HUMAN | Polypeptide N-acetylgalactosaminylt... | GALNT3 | 25 | Annotation score: | ||
C9JY57 | C9JY57_HUMAN | Polypeptide N-acetylgalactosaminylt... | GALNT3 | 11 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 481 | Q → R in CAA63371 (PubMed:8663203).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_080832 | 162 – 633 | Missing in HFTC1. 1 PublicationAdd BLAST | 472 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_011202 | 173 – 192 | CIEQK…SVIIV → YVEEYLLFILYHQALQGREG in isoform 2. 1 PublicationAdd BLAST | 20 | |
Alternative sequenceiVSP_011203 | 193 – 633 | Missing in isoform 2. 1 PublicationAdd BLAST | 441 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X92689 mRNA Translation: CAA63371.1 AC009495 Genomic DNA Translation: AAY14678.1 CH471058 Genomic DNA Translation: EAX11324.1 BC056246 mRNA Translation: AAH56246.1 Different initiation. BC113565 mRNA Translation: AAI13566.1 BC113567 mRNA Translation: AAI13568.1 |
CCDSi | CCDS2226.1 [Q14435-1] |
RefSeqi | NP_004473.2, NM_004482.3 [Q14435-1] XP_005246506.1, XM_005246449.1 [Q14435-1] XP_011509231.1, XM_011510929.1 [Q14435-1] XP_016859259.1, XM_017003770.1 [Q14435-1] |
Genome annotation databases
Ensembli | ENST00000392701.8; ENSP00000376465.3; ENSG00000115339.14 |
GeneIDi | 2591 |
KEGGi | hsa:2591 |
MANE-Selecti | ENST00000392701.8; ENSP00000376465.3; NM_004482.4; NP_004473.2 |
UCSCi | uc010fph.2, human [Q14435-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
Functional Glycomics Gateway - GTase Polypeptide N-acetylgalactosaminyltransferase 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X92689 mRNA Translation: CAA63371.1 AC009495 Genomic DNA Translation: AAY14678.1 CH471058 Genomic DNA Translation: EAX11324.1 BC056246 mRNA Translation: AAH56246.1 Different initiation. BC113565 mRNA Translation: AAI13566.1 BC113567 mRNA Translation: AAI13568.1 |
CCDSi | CCDS2226.1 [Q14435-1] |
RefSeqi | NP_004473.2, NM_004482.3 [Q14435-1] XP_005246506.1, XM_005246449.1 [Q14435-1] XP_011509231.1, XM_011510929.1 [Q14435-1] XP_016859259.1, XM_017003770.1 [Q14435-1] |
3D structure databases
AlphaFoldDBi | Q14435 |
SMRi | Q14435 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 108863, 25 interactors |
IntActi | Q14435, 6 interactors |
STRINGi | 9606.ENSP00000376465 |
Chemistry databases
BindingDBi | Q14435 |
ChEMBLi | CHEMBL4523291 |
Protein family/group databases
CAZyi | CBM13, Carbohydrate-Binding Module Family 13 GT27, Glycosyltransferase Family 27 |
PTM databases
GlyConnecti | 1620, 3 N-Linked glycans (1 site) |
GlyGeni | Q14435, 3 sites, 3 N-linked glycans (1 site) |
iPTMneti | Q14435 |
PhosphoSitePlusi | Q14435 |
Genetic variation databases
BioMutai | GALNT3 |
DMDMi | 209572629 |
Proteomic databases
EPDi | Q14435 |
jPOSTi | Q14435 |
MassIVEi | Q14435 |
MaxQBi | Q14435 |
PaxDbi | Q14435 |
PeptideAtlasi | Q14435 |
PRIDEi | Q14435 |
ProteomicsDBi | 59988 [Q14435-1] 59989 [Q14435-2] |
Protocols and materials databases
Antibodypediai | 2358, 171 antibodies from 25 providers |
DNASUi | 2591 |
Genome annotation databases
Ensembli | ENST00000392701.8; ENSP00000376465.3; ENSG00000115339.14 |
GeneIDi | 2591 |
KEGGi | hsa:2591 |
MANE-Selecti | ENST00000392701.8; ENSP00000376465.3; NM_004482.4; NP_004473.2 |
UCSCi | uc010fph.2, human [Q14435-1] |
Organism-specific databases
CTDi | 2591 |
DisGeNETi | 2591 |
GeneCardsi | GALNT3 |
GeneReviewsi | GALNT3 |
HGNCi | HGNC:4125, GALNT3 |
HPAi | ENSG00000115339, Tissue enhanced (stomach) |
MalaCardsi | GALNT3 |
MIMi | 211900, phenotype 601756, gene |
neXtProti | NX_Q14435 |
OpenTargetsi | ENSG00000115339 |
Orphaneti | 306661, Familial hyperphosphatemic tumoral calcinosis/Hyperphosphatemic hyperostosis syndrome |
PharmGKBi | PA28538 |
VEuPathDBi | HostDB:ENSG00000115339 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3736, Eukaryota |
GeneTreei | ENSGT00940000156609 |
InParanoidi | Q14435 |
OMAi | NGEHPNL |
OrthoDBi | 606683at2759 |
PhylomeDBi | Q14435 |
TreeFami | TF313267 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
BRENDAi | 2.4.1.41, 2681 |
PathwayCommonsi | Q14435 |
Reactomei | R-HSA-190372, FGFR3c ligand binding and activation R-HSA-5083625, Defective GALNT3 causes HFTC R-HSA-913709, O-linked glycosylation of mucins |
SignaLinki | Q14435 |
Miscellaneous databases
BioGRID-ORCSi | 2591, 11 hits in 1067 CRISPR screens |
ChiTaRSi | GALNT3, human |
GeneWikii | GALNT3 |
GenomeRNAii | 2591 |
Pharosi | Q14435, Tbio |
PROi | PR:Q14435 |
RNActi | Q14435, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000115339, Expressed in colonic mucosa and 189 other tissues |
ExpressionAtlasi | Q14435, baseline and differential |
Genevisiblei | Q14435, HS |
Family and domain databases
CDDi | cd02510, pp-GalNAc-T, 1 hit cd00161, RICIN, 1 hit |
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR045885, GalNAc-T IPR001173, Glyco_trans_2-like IPR029044, Nucleotide-diphossugar_trans IPR035992, Ricin_B-like_lectins IPR000772, Ricin_B_lectin |
Pfami | View protein in Pfam PF00535, Glycos_transf_2, 1 hit PF00652, Ricin_B_lectin, 1 hit |
SMARTi | View protein in SMART SM00458, RICIN, 1 hit |
SUPFAMi | SSF50370, SSF50370, 1 hit SSF53448, SSF53448, 1 hit |
PROSITEi | View protein in PROSITE PS50231, RICIN_B_LECTIN, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GALT3_HUMAN | |
Accessioni | Q14435Primary (citable) accession number: Q14435 Secondary accession number(s): Q53TG9, Q7Z476 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 16, 2004 |
Last sequence update: | October 14, 2008 | |
Last modified: | May 25, 2022 | |
This is version 186 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families