UniProtKB - Q14344 (GNA13_HUMAN)
Guanine nucleotide-binding protein subunit alpha-13
GNA13
Functioni
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 62 | MagnesiumBy similarity | 1 | |
Binding sitei | 173 | GTPBy similarity | 1 | |
Metal bindingi | 203 | MagnesiumBy similarity | 1 | |
Binding sitei | 349 | GTP; via amide nitrogenBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 58 – 63 | GTPBy similarity | 6 | |
Nucleotide bindingi | 197 – 200 | GTPBy similarity | 4 | |
Nucleotide bindingi | 291 – 294 | GTPBy similarity | 4 |
GO - Molecular functioni
- D5 dopamine receptor binding Source: GO_Central
- G-protein beta/gamma-subunit complex binding Source: GO_Central
- G protein-coupled receptor binding Source: GO_Central
- GTPase activity Source: GO_Central
- GTP binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- activation of phospholipase D activity Source: GO_Central
- adenylate cyclase-activating G protein-coupled receptor signaling pathway Source: GO_Central
- adenylate cyclase-modulating G protein-coupled receptor signaling pathway Source: UniProtKB
- branching involved in blood vessel morphogenesis Source: Ensembl
- cell differentiation Source: Ensembl
- G protein-coupled receptor signaling pathway Source: Reactome
- in utero embryonic development Source: Ensembl
- platelet activation Source: Reactome
- positive regulation of apoptotic process Source: Reactome
- positive regulation of cytosolic calcium ion concentration Source: Ensembl
- regulation of cell migration Source: Ensembl
- regulation of cell shape Source: Ensembl
- regulation of small GTPase mediated signal transduction Source: Reactome
- Rho protein signal transduction Source: GO_Central
- signal transduction Source: ProtInc
Keywordsi
Molecular function | Transducer |
Ligand | GTP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | Q14344 |
Reactomei | R-HSA-193648, NRAGE signals death through JNK R-HSA-194840, Rho GTPase cycle R-HSA-416482, G alpha (12/13) signalling events R-HSA-428930, Thromboxane signalling through TP receptor R-HSA-456926, Thrombin signalling through proteinase activated receptors (PARs) |
SignaLinki | Q14344 |
SIGNORi | Q14344 |
Names & Taxonomyi
Protein namesi | Recommended name: Guanine nucleotide-binding protein subunit alpha-13Short name: G alpha-13 Short name: G-protein subunit alpha-13 |
Gene namesi | Name:GNA13 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:4381, GNA13 |
MIMi | 604406, gene |
neXtProti | NX_Q14344 |
VEuPathDBi | HostDB:ENSG00000120063.9 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Lipid-anchor 1 Publication
Nucleus
- Nucleus 1 Publication
Other locations
- Melanosome 1 Publication
- Cytoplasm 2 Publications
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065). Detected in the cytoplasm of Leydig cells and in the seminiferous epithelium, including differentiating cells from the spermatogonia to mature spermatozoa stages (PubMed:18703424). In round spermatids, also present in the nuclei (PubMed:18703424).2 Publications
Cytosol
- cytosol Source: HPA
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Nucleus
- nucleus Source: UniProtKB
Plasma Membrane
- brush border membrane Source: GO_Central
- heterotrimeric G-protein complex Source: GO_Central
- plasma membrane Source: Reactome
Other locations
- cytoplasm Source: UniProtKB
- focal adhesion Source: UniProtKB
- melanosome Source: UniProtKB-SubCell
- membrane Source: UniProtKB
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Membrane, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 14 | C → S: Fails to localize to plasma membranes and failed to activate Rho-dependent serum response factor-mediated transcription and actin stress fiber formation. 1 Publication | 1 | |
Mutagenesisi | 18 | C → S: Fails to localize to plasma membranes and failed to activate Rho-dependent serum response factor-mediated transcription and actin stress fiber formation. 1 Publication | 1 | |
Mutagenesisi | 203 | T → A: Abolishes phosphorylation by PKA; disrupts heterotrimer stability. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 10672 |
OpenTargetsi | ENSG00000120063 |
PharmGKBi | PA28766 |
Miscellaneous databases
Pharosi | Q14344, Tbio |
Genetic variation databases
BioMutai | GNA13 |
DMDMi | 38258936 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000203773 | 1 – 377 | Guanine nucleotide-binding protein subunit alpha-13Add BLAST | 377 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 14 | S-palmitoyl cysteine1 Publication | 1 | |
Lipidationi | 18 | S-palmitoyl cysteine1 Publication | 1 | |
Modified residuei | 203 | Phosphothreonine; by PKA1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Lipoprotein, Palmitate, PhosphoproteinProteomic databases
EPDi | Q14344 |
jPOSTi | Q14344 |
MassIVEi | Q14344 |
MaxQBi | Q14344 |
PaxDbi | Q14344 |
PeptideAtlasi | Q14344 |
PRIDEi | Q14344 |
ProteomicsDBi | 25652 59969 [Q14344-1] |
PTM databases
iPTMneti | Q14344 |
MetOSitei | Q14344 |
PhosphoSitePlusi | Q14344 |
SwissPalmi | Q14344 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000120063, Expressed in blood and 232 other tissues |
ExpressionAtlasi | Q14344, baseline and differential |
Genevisiblei | Q14344, HS |
Organism-specific databases
HPAi | ENSG00000120063, Tissue enhanced (bone) |
Interactioni
Subunit structurei
G proteins are composed of 3 units; alpha, beta and gamma (PubMed:12399457). The alpha chain contains the guanine nucleotide binding site (By similarity).
Interacts with UBXD5 (PubMed:16202387).
Interacts with HAX1 (PubMed:15339924).
Interacts (in GTP-bound form) with PPP5C (via TPR repeats); activates PPP5C phosphatase activity and translocates PPP5C to the cell membrane.
Interacts with RGS22 (PubMed:18703424).
Interacts (in GTP-bound form) with ARHGEF1 (PubMed:10747909).
Interacts (in GTP-bound form) with ARHGEF11 (via RGS domain) (PubMed:10026210).
Interacts (in GTP-bound form) with ARHGEF12 (via RGS domain) (PubMed:11094164).
Interacts with CTNND1 (PubMed:15240885).
Interacts with GASL2L2 (PubMed:23994616).
By similarity9 PublicationsBinary interactionsi
Hide detailsGO - Molecular functioni
- D5 dopamine receptor binding Source: GO_Central
- G protein-coupled receptor binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 115914, 82 interactors |
CORUMi | Q14344 |
IntActi | Q14344, 27 interactors |
MINTi | Q14344 |
STRINGi | 9606.ENSP00000400717 |
Miscellaneous databases
RNActi | Q14344, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 47 – 377 | G-alphaPROSITE-ProRule annotationAdd BLAST | 331 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 50 – 63 | G1 motifPROSITE-ProRule annotationAdd BLAST | 14 | |
Regioni | 195 – 203 | G2 motifPROSITE-ProRule annotation | 9 | |
Regioni | 218 – 227 | G3 motifPROSITE-ProRule annotation | 10 | |
Regioni | 287 – 294 | G4 motifPROSITE-ProRule annotation | 8 | |
Regioni | 347 – 352 | G5 motifPROSITE-ProRule annotation | 6 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0082, Eukaryota |
GeneTreei | ENSGT00940000157054 |
HOGENOMi | CLU_014184_3_1_1 |
InParanoidi | Q14344 |
OMAi | PWGDPAN |
PhylomeDBi | Q14344 |
TreeFami | TF300673 |
Family and domain databases
CDDi | cd00066, G-alpha, 1 hit |
Gene3Di | 1.10.400.10, 1 hit |
InterProi | View protein in InterPro IPR000469, Gprotein_alpha_12/13 IPR001019, Gprotein_alpha_su IPR011025, GproteinA_insert IPR027417, P-loop_NTPase |
PANTHERi | PTHR10218, PTHR10218, 1 hit |
Pfami | View protein in Pfam PF00503, G-alpha, 1 hit |
PRINTSi | PR00318, GPROTEINA PR00440, GPROTEINA12 |
SMARTi | View protein in SMART SM00275, G_alpha, 1 hit |
SUPFAMi | SSF47895, SSF47895, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51882, G_ALPHA, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MADFLPSRSV LSVCFPGCLL TSGEAEQQRK SKEIDKCLSR EKTYVKRLVK
60 70 80 90 100
ILLLGAGESG KSTFLKQMRI IHGQDFDQRA REEFRPTIYS NVIKGMRVLV
110 120 130 140 150
DAREKLHIPW GDNSNQQHGD KMMSFDTRAP MAAQGMVETR VFLQYLPAIR
160 170 180 190 200
ALWADSGIQN AYDRRREFQL GESVKYFLDN LDKLGEPDYI PSQQDILLAR
210 220 230 240 250
RPTKGIHEYD FEIKNVPFKM VDVGGQRSER KRWFECFDSV TSILFLVSSS
260 270 280 290 300
EFDQVLMEDR LTNRLTESLN IFETIVNNRV FSNVSIILFL NKTDLLEEKV
310 320 330 340 350
QIVSIKDYFL EFEGDPHCLR DVQKFLVECF RNKRRDQQQK PLYHHFTTAI
360 370
NTENIRLVFR DVKDTILHDN LKQLMLQ
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 211 | F → L in BAH13696 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_017160 | 221 | V → L1 PublicationCorresponds to variant dbSNP:rs1062597Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_055140 | 1 – 95 | Missing in isoform 2. 1 PublicationAdd BLAST | 95 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L22075 mRNA Translation: AAA74235.1 AF493902 mRNA Translation: AAM12616.1 AK302400 mRNA Translation: BAH13696.1 AK313672 mRNA Translation: BAG36424.1 AC037487 Genomic DNA No translation available. CH471099 Genomic DNA Translation: EAW88993.1 BC036756 mRNA Translation: AAH36756.1 |
CCDSi | CCDS11661.1 [Q14344-1] CCDS62302.1 [Q14344-2] |
PIRi | I57490 |
RefSeqi | NP_001269354.1, NM_001282425.1 [Q14344-2] NP_006563.2, NM_006572.5 [Q14344-1] |
Genome annotation databases
Ensembli | ENST00000439174; ENSP00000400717; ENSG00000120063 [Q14344-1] ENST00000541118; ENSP00000439647; ENSG00000120063 [Q14344-2] |
GeneIDi | 10672 |
KEGGi | hsa:10672 |
UCSCi | uc002jfc.4, human [Q14344-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L22075 mRNA Translation: AAA74235.1 AF493902 mRNA Translation: AAM12616.1 AK302400 mRNA Translation: BAH13696.1 AK313672 mRNA Translation: BAG36424.1 AC037487 Genomic DNA No translation available. CH471099 Genomic DNA Translation: EAW88993.1 BC036756 mRNA Translation: AAH36756.1 |
CCDSi | CCDS11661.1 [Q14344-1] CCDS62302.1 [Q14344-2] |
PIRi | I57490 |
RefSeqi | NP_001269354.1, NM_001282425.1 [Q14344-2] NP_006563.2, NM_006572.5 [Q14344-1] |
3D structure databases
SMRi | Q14344 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 115914, 82 interactors |
CORUMi | Q14344 |
IntActi | Q14344, 27 interactors |
MINTi | Q14344 |
STRINGi | 9606.ENSP00000400717 |
PTM databases
iPTMneti | Q14344 |
MetOSitei | Q14344 |
PhosphoSitePlusi | Q14344 |
SwissPalmi | Q14344 |
Genetic variation databases
BioMutai | GNA13 |
DMDMi | 38258936 |
Proteomic databases
EPDi | Q14344 |
jPOSTi | Q14344 |
MassIVEi | Q14344 |
MaxQBi | Q14344 |
PaxDbi | Q14344 |
PeptideAtlasi | Q14344 |
PRIDEi | Q14344 |
ProteomicsDBi | 25652 59969 [Q14344-1] |
Protocols and materials databases
Antibodypediai | 31626, 259 antibodies |
DNASUi | 10672 |
Genome annotation databases
Ensembli | ENST00000439174; ENSP00000400717; ENSG00000120063 [Q14344-1] ENST00000541118; ENSP00000439647; ENSG00000120063 [Q14344-2] |
GeneIDi | 10672 |
KEGGi | hsa:10672 |
UCSCi | uc002jfc.4, human [Q14344-1] |
Organism-specific databases
CTDi | 10672 |
DisGeNETi | 10672 |
GeneCardsi | GNA13 |
HGNCi | HGNC:4381, GNA13 |
HPAi | ENSG00000120063, Tissue enhanced (bone) |
MIMi | 604406, gene |
neXtProti | NX_Q14344 |
OpenTargetsi | ENSG00000120063 |
PharmGKBi | PA28766 |
VEuPathDBi | HostDB:ENSG00000120063.9 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0082, Eukaryota |
GeneTreei | ENSGT00940000157054 |
HOGENOMi | CLU_014184_3_1_1 |
InParanoidi | Q14344 |
OMAi | PWGDPAN |
PhylomeDBi | Q14344 |
TreeFami | TF300673 |
Enzyme and pathway databases
PathwayCommonsi | Q14344 |
Reactomei | R-HSA-193648, NRAGE signals death through JNK R-HSA-194840, Rho GTPase cycle R-HSA-416482, G alpha (12/13) signalling events R-HSA-428930, Thromboxane signalling through TP receptor R-HSA-456926, Thrombin signalling through proteinase activated receptors (PARs) |
SignaLinki | Q14344 |
SIGNORi | Q14344 |
Miscellaneous databases
BioGRID-ORCSi | 10672, 24 hits in 875 CRISPR screens |
ChiTaRSi | GNA13, human |
GeneWikii | GNA13 |
GenomeRNAii | 10672 |
Pharosi | Q14344, Tbio |
PROi | PR:Q14344 |
RNActi | Q14344, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000120063, Expressed in blood and 232 other tissues |
ExpressionAtlasi | Q14344, baseline and differential |
Genevisiblei | Q14344, HS |
Family and domain databases
CDDi | cd00066, G-alpha, 1 hit |
Gene3Di | 1.10.400.10, 1 hit |
InterProi | View protein in InterPro IPR000469, Gprotein_alpha_12/13 IPR001019, Gprotein_alpha_su IPR011025, GproteinA_insert IPR027417, P-loop_NTPase |
PANTHERi | PTHR10218, PTHR10218, 1 hit |
Pfami | View protein in Pfam PF00503, G-alpha, 1 hit |
PRINTSi | PR00318, GPROTEINA PR00440, GPROTEINA12 |
SMARTi | View protein in SMART SM00275, G_alpha, 1 hit |
SUPFAMi | SSF47895, SSF47895, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51882, G_ALPHA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GNA13_HUMAN | |
Accessioni | Q14344Primary (citable) accession number: Q14344 Secondary accession number(s): B2R977 Q8TD70 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | October 31, 2003 | |
Last modified: | February 10, 2021 | |
This is version 194 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 17
Human chromosome 17: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families