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Protein

Guanine nucleotide-binding protein subunit alpha-13

Gene

GNA13

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems (PubMed:15240885, PubMed:16787920, PubMed:16705036, PubMed:27084452). Activates effector molecule RhoA by binding and activating RhoGEFs (ARHGEF1/p115RhoGEF, ARHGEF11/PDZ-RhoGEF and ARHGEF12/LARG) (PubMed:15240885, PubMed:12515866). GNA13-dependent Rho signaling subsequently regulates transcription factor AP-1 (activating protein-1) (By similarity). Promotes tumor cell invasion and metastasis by activating RhoA/ROCK signaling pathway (PubMed:16787920, PubMed:16705036, PubMed:27084452). Inhibits CDH1-mediated cell adhesion in process independent from Rho activation (PubMed:11976333).By similarity6 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi62MagnesiumBy similarity1
Binding sitei173GTPBy similarity1
Metal bindingi203MagnesiumBy similarity1
Metal bindingi222MagnesiumBy similarity1
Binding sitei349GTP; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi58 – 63GTPBy similarity6
Nucleotide bindingi197 – 200GTPBy similarity4
Nucleotide bindingi291 – 294GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransducer
LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-193648 NRAGE signals death through JNK
R-HSA-194840 Rho GTPase cycle
R-HSA-416482 G alpha (12/13) signalling events
R-HSA-428930 Thromboxane signalling through TP receptor
R-HSA-456926 Thrombin signalling through proteinase activated receptors (PARs)
SignaLinkiQ14344
SIGNORiQ14344

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein subunit alpha-13
Short name:
G alpha-13
Short name:
G-protein subunit alpha-13
Gene namesi
Name:GNA13
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000120063.9
HGNCiHGNC:4381 GNA13
MIMi604406 gene
neXtProtiNX_Q14344

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi14C → S: Fails to localize to plasma membranes and failed to activate Rho-dependent serum response factor-mediated transcription and actin stress fiber formation. 1 Publication1
Mutagenesisi18C → S: Fails to localize to plasma membranes and failed to activate Rho-dependent serum response factor-mediated transcription and actin stress fiber formation. 1 Publication1
Mutagenesisi203T → A: Abolishes phosphorylation by PKA; disrupts heterotrimer stability. 1 Publication1

Organism-specific databases

DisGeNETi10672
OpenTargetsiENSG00000120063
PharmGKBiPA28766

Polymorphism and mutation databases

DMDMi38258936

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002037731 – 377Guanine nucleotide-binding protein subunit alpha-13Add BLAST377

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi14S-palmitoyl cysteine1 Publication1
Lipidationi18S-palmitoyl cysteine1 Publication1
Modified residuei203Phosphothreonine; by PKA1 Publication1

Post-translational modificationi

Palmitoylation is critical for proper membrane localization and signaling.1 Publication
Phosphorylation on Thr-203 by PKA destabilizes the heterotrimer of alpha, beta and gamma, and inhibits Rho activation.1 Publication

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

EPDiQ14344
MaxQBiQ14344
PaxDbiQ14344
PeptideAtlasiQ14344
PRIDEiQ14344
ProteomicsDBi59969

PTM databases

iPTMnetiQ14344
PhosphoSitePlusiQ14344
SwissPalmiQ14344

Expressioni

Tissue specificityi

Expressed in testis, including in Leydig cells and in the seminiferous epithelium, in differentiating cells from the spermatogonia to mature spermatozoa stages and round spermatids (at protein level). Expressed in 99.2% of spermatozoa from healthy individuals, but only in 28.6% of macrocephalic spermatozoa from infertile patients (at protein level).1 Publication

Gene expression databases

BgeeiENSG00000120063 Expressed in 219 organ(s), highest expression level in blood
CleanExiHS_GNA13
ExpressionAtlasiQ14344 baseline and differential
GenevisibleiQ14344 HS

Organism-specific databases

HPAiHPA010087

Interactioni

Subunit structurei

G proteins are composed of 3 units; alpha, beta and gamma (PubMed:12399457). The alpha chain contains the guanine nucleotide binding site (By similarity). Interacts with UBXD5 (PubMed:16202387). Interacts with HAX1 (PubMed:15339924). Interacts (in GTP-bound form) with PPP5C (via TPR repeats); activates PPP5C phosphatase activity and translocates PPP5C to the cell membrane. Interacts with RGS22 (PubMed:18703424). Interacts (in GTP-bound form) with ARHGEF1 (PubMed:10747909). Interacts (in GTP-bound form) with ARHGEF11 (via RGS domain) (PubMed:10026210). Interacts (in GTP-bound form) with ARHGEF12 (via RGS domain) (PubMed:11094164). Interacts with CTNND1 (PubMed:15240885).By similarity8 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi115914, 71 interactors
CORUMiQ14344
IntActiQ14344, 11 interactors
MINTiQ14344
STRINGi9606.ENSP00000400717

Structurei

3D structure databases

ProteinModelPortaliQ14344
SMRiQ14344
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-alpha family. G(12) subfamily.Curated

Phylogenomic databases

eggNOGiKOG0082 Eukaryota
ENOG410XNVQ LUCA
GeneTreeiENSGT00760000118851
HOGENOMiHOG000038729
HOVERGENiHBG063184
InParanoidiQ14344
KOiK04639
OMAiCFPGCVL
OrthoDBiEOG091G0NV2
PhylomeDBiQ14344
TreeFamiTF300673

Family and domain databases

CDDicd00066 G-alpha, 1 hit
Gene3Di1.10.400.10, 1 hit
InterProiView protein in InterPro
IPR000469 Gprotein_alpha_12/13
IPR001019 Gprotein_alpha_su
IPR011025 GproteinA_insert
IPR027417 P-loop_NTPase
PANTHERiPTHR10218 PTHR10218, 1 hit
PfamiView protein in Pfam
PF00503 G-alpha, 1 hit
PRINTSiPR00318 GPROTEINA
PR00440 GPROTEINA12
SMARTiView protein in SMART
SM00275 G_alpha, 1 hit
SUPFAMiSSF47895 SSF47895, 1 hit
SSF52540 SSF52540, 2 hits

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q14344-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MADFLPSRSV LSVCFPGCLL TSGEAEQQRK SKEIDKCLSR EKTYVKRLVK
60 70 80 90 100
ILLLGAGESG KSTFLKQMRI IHGQDFDQRA REEFRPTIYS NVIKGMRVLV
110 120 130 140 150
DAREKLHIPW GDNSNQQHGD KMMSFDTRAP MAAQGMVETR VFLQYLPAIR
160 170 180 190 200
ALWADSGIQN AYDRRREFQL GESVKYFLDN LDKLGEPDYI PSQQDILLAR
210 220 230 240 250
RPTKGIHEYD FEIKNVPFKM VDVGGQRSER KRWFECFDSV TSILFLVSSS
260 270 280 290 300
EFDQVLMEDR LTNRLTESLN IFETIVNNRV FSNVSIILFL NKTDLLEEKV
310 320 330 340 350
QIVSIKDYFL EFEGDPHCLR DVQKFLVECF RNKRRDQQQK PLYHHFTTAI
360 370
NTENIRLVFR DVKDTILHDN LKQLMLQ
Length:377
Mass (Da):44,050
Last modified:October 31, 2003 - v2
Checksum:i929B7B6473C54F2E
GO
Isoform 2 (identifier: Q14344-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-95: Missing.

Show »
Length:282
Mass (Da):33,287
Checksum:iE32B6298504794BB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti211F → L in BAH13696 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_017160221V → L1 PublicationCorresponds to variant dbSNP:rs1062597Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0551401 – 95Missing in isoform 2. 1 PublicationAdd BLAST95

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22075 mRNA Translation: AAA74235.1
AF493902 mRNA Translation: AAM12616.1
AK302400 mRNA Translation: BAH13696.1
AK313672 mRNA Translation: BAG36424.1
AC037487 Genomic DNA No translation available.
CH471099 Genomic DNA Translation: EAW88993.1
BC036756 mRNA Translation: AAH36756.1
CCDSiCCDS11661.1 [Q14344-1]
CCDS62302.1 [Q14344-2]
PIRiI57490
RefSeqiNP_001269354.1, NM_001282425.1 [Q14344-2]
NP_006563.2, NM_006572.5 [Q14344-1]
UniGeneiHs.515018

Genome annotation databases

EnsembliENST00000439174; ENSP00000400717; ENSG00000120063 [Q14344-1]
ENST00000541118; ENSP00000439647; ENSG00000120063 [Q14344-2]
GeneIDi10672
KEGGihsa:10672
UCSCiuc002jfc.4 human [Q14344-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22075 mRNA Translation: AAA74235.1
AF493902 mRNA Translation: AAM12616.1
AK302400 mRNA Translation: BAH13696.1
AK313672 mRNA Translation: BAG36424.1
AC037487 Genomic DNA No translation available.
CH471099 Genomic DNA Translation: EAW88993.1
BC036756 mRNA Translation: AAH36756.1
CCDSiCCDS11661.1 [Q14344-1]
CCDS62302.1 [Q14344-2]
PIRiI57490
RefSeqiNP_001269354.1, NM_001282425.1 [Q14344-2]
NP_006563.2, NM_006572.5 [Q14344-1]
UniGeneiHs.515018

3D structure databases

ProteinModelPortaliQ14344
SMRiQ14344
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115914, 71 interactors
CORUMiQ14344
IntActiQ14344, 11 interactors
MINTiQ14344
STRINGi9606.ENSP00000400717

PTM databases

iPTMnetiQ14344
PhosphoSitePlusiQ14344
SwissPalmiQ14344

Polymorphism and mutation databases

DMDMi38258936

Proteomic databases

EPDiQ14344
MaxQBiQ14344
PaxDbiQ14344
PeptideAtlasiQ14344
PRIDEiQ14344
ProteomicsDBi59969

Protocols and materials databases

DNASUi10672
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000439174; ENSP00000400717; ENSG00000120063 [Q14344-1]
ENST00000541118; ENSP00000439647; ENSG00000120063 [Q14344-2]
GeneIDi10672
KEGGihsa:10672
UCSCiuc002jfc.4 human [Q14344-1]

Organism-specific databases

CTDi10672
DisGeNETi10672
EuPathDBiHostDB:ENSG00000120063.9
GeneCardsiGNA13
HGNCiHGNC:4381 GNA13
HPAiHPA010087
MIMi604406 gene
neXtProtiNX_Q14344
OpenTargetsiENSG00000120063
PharmGKBiPA28766
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0082 Eukaryota
ENOG410XNVQ LUCA
GeneTreeiENSGT00760000118851
HOGENOMiHOG000038729
HOVERGENiHBG063184
InParanoidiQ14344
KOiK04639
OMAiCFPGCVL
OrthoDBiEOG091G0NV2
PhylomeDBiQ14344
TreeFamiTF300673

Enzyme and pathway databases

ReactomeiR-HSA-193648 NRAGE signals death through JNK
R-HSA-194840 Rho GTPase cycle
R-HSA-416482 G alpha (12/13) signalling events
R-HSA-428930 Thromboxane signalling through TP receptor
R-HSA-456926 Thrombin signalling through proteinase activated receptors (PARs)
SignaLinkiQ14344
SIGNORiQ14344

Miscellaneous databases

ChiTaRSiGNA13 human
GeneWikiiGNA13
GenomeRNAii10672
PROiPR:Q14344
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000120063 Expressed in 219 organ(s), highest expression level in blood
CleanExiHS_GNA13
ExpressionAtlasiQ14344 baseline and differential
GenevisibleiQ14344 HS

Family and domain databases

CDDicd00066 G-alpha, 1 hit
Gene3Di1.10.400.10, 1 hit
InterProiView protein in InterPro
IPR000469 Gprotein_alpha_12/13
IPR001019 Gprotein_alpha_su
IPR011025 GproteinA_insert
IPR027417 P-loop_NTPase
PANTHERiPTHR10218 PTHR10218, 1 hit
PfamiView protein in Pfam
PF00503 G-alpha, 1 hit
PRINTSiPR00318 GPROTEINA
PR00440 GPROTEINA12
SMARTiView protein in SMART
SM00275 G_alpha, 1 hit
SUPFAMiSSF47895 SSF47895, 1 hit
SSF52540 SSF52540, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiGNA13_HUMAN
AccessioniPrimary (citable) accession number: Q14344
Secondary accession number(s): B2R977
, B7Z7R0, F5H1G8, Q8TD70
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: October 31, 2003
Last modified: October 10, 2018
This is version 175 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  2. SIMILARITY comments
    Index of protein domains and families
  3. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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