UniProtKB - Q14296 (FASTK_HUMAN)
Fas-activated serine/threonine kinase
FASTK
Functioni
Phosphorylates the splicing regulator TIA1, thereby promoting the inclusion of FAS exon 6, which leads to an mRNA encoding a pro-apoptotic form of the receptor.
2 PublicationsRequired for the biogenesis of some mitochondrial-encoded mRNAs, specifically stabilizes ND6 (NADH dehydrogenase complex subunit 6) mRNA, and regulates its levels.
1 PublicationCatalytic activityi
- ATP + L-seryl-[Fas-activated protein] = ADP + H+ + O-phospho-L-seryl-[Fas-activated protein]1 PublicationEC:2.7.11.81 Publication
- ATP + L-threonyl-[Fas-activated protein] = ADP + H+ + O-phospho-L-threonyl-[Fas-activated protein]1 PublicationEC:2.7.11.81 Publication
- EC:2.7.11.11 Publication
- EC:2.7.11.11 Publication
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- Fas-activated serine/threonine kinase activity Source: UniProtKB-EC
- protein serine/threonine kinase activity Source: UniProtKB
- RNA binding Source: GO_Central
GO - Biological processi
- apoptotic signaling pathway Source: ProtInc
- mitochondrial RNA processing Source: GO_Central
- protein phosphorylation Source: UniProtKB
- regulation of mitochondrial mRNA stability Source: GO_Central
- regulation of RNA splicing Source: UniProtKB
Keywordsi
Molecular function | Kinase, RNA-binding, Serine/threonine-protein kinase, Transferase |
Biological process | Apoptosis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.8, 2681 |
PathwayCommonsi | Q14296 |
SignaLinki | Q14296 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:FASTK |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:24676, FASTK |
MIMi | 606965, gene |
neXtProti | NX_Q14296 |
VEuPathDBi | HostDB:ENSG00000164896 |
Subcellular locationi
Mitochondrion
- Mitochondrion matrix 1 Publication
Note: Colocalizes with mitochondrial RNA granules.1 Publication
Mitochondrion
- mitochondrial matrix Source: GO_Central
Other locations
- ribonucleoprotein granule Source: GO_Central
Keywords - Cellular componenti
MitochondrionPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 35 | M → G: Abolishes isoform 4 expression. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 10922 |
OpenTargetsi | ENSG00000164896 |
PharmGKBi | PA142671775 |
Miscellaneous databases
Pharosi | Q14296, Tbio |
Genetic variation databases
BioMutai | FASTK |
DMDMi | 13124202 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000087196 | 1 – 549 | Fas-activated serine/threonine kinaseAdd BLAST | 549 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | Q14296 |
MassIVEi | Q14296 |
MaxQBi | Q14296 |
PaxDbi | Q14296 |
PeptideAtlasi | Q14296 |
PRIDEi | Q14296 |
ProteomicsDBi | 28667 59957 [Q14296-1] 59958 [Q14296-2] |
PTM databases
GlyGeni | Q14296, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q14296 |
PhosphoSitePlusi | Q14296 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000164896, Expressed in apex of heart and 237 other tissues |
ExpressionAtlasi | Q14296, baseline and differential |
Genevisiblei | Q14296, HS |
Organism-specific databases
HPAi | ENSG00000164896, Low tissue specificity |
Interactioni
Subunit structurei
Binary interactionsi
Q14296
Protein-protein interaction databases
BioGRIDi | 116126, 14 interactors |
IntActi | Q14296, 13 interactors |
STRINGi | 9606.ENSP00000297532 |
Miscellaneous databases
RNActi | Q14296, protein |
Structurei
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 477 – 535 | RAPPROSITE-ProRule annotationAdd BLAST | 59 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 30 | DisorderedSequence analysisAdd BLAST | 30 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG502QXK7, Eukaryota |
GeneTreei | ENSGT01030000234607 |
HOGENOMi | CLU_046861_0_0_1 |
InParanoidi | Q14296 |
OMAi | KVRTHHY |
PhylomeDBi | Q14296 |
TreeFami | TF331796 |
Family and domain databases
InterProi | View protein in InterPro IPR013579, FAST_2 IPR010622, FAST_Leu-rich IPR013584, RAP IPR015715, Ser/Thr_kinase_FAST |
PANTHERi | PTHR21228:SF4, PTHR21228:SF4, 1 hit |
Pfami | View protein in Pfam PF06743, FAST_1, 1 hit PF08368, FAST_2, 1 hit PF08373, RAP, 1 hit |
SMARTi | View protein in SMART SM00952, RAP, 1 hit |
PROSITEi | View protein in PROSITE PS51286, RAP, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing and alternative initiation. AlignAdd to basketThis entry has 4 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MRRPRGEPGP RAPRPTEGAT CAGPGESWSP SPNSMLRVLL SAQTSPARLS
60 70 80 90 100
GLLLIPPVQP CCLGPSKWGD RPVGGGPSAG PVQGLQRLLE QAKSPGELLR
110 120 130 140 150
WLGQNPSKVR AHHYSVALRR LGQLLGSRPR PPPVEQVTLQ DLSQLIIRNC
160 170 180 190 200
PSFDIHTIHV CLHLAVLLGF PSDGPLVCAL EQERRLRLPP KPPPPLQPLL
210 220 230 240 250
RGGQGLEAAL SCPRFLRYPR QHLISSLAEA RPEELTPHVM VLLAQHLARH
260 270 280 290 300
RLREPQLLEA IAHFLVVQET QLSSKVVQKL VLPFGRLNYL PLEQQFMPCL
310 320 330 340 350
ERILAREAGV APLATVNILM SLCQLRCLPF RALHFVFSPG FINYISGTPH
360 370 380 390 400
ALIVRRYLSL LDTAVELELP GYRGPRLPRR QQVPIFPQPL ITDRARCKYS
410 420 430 440 450
HKDIVAEGLR QLLGEEKYRQ DLTVPPGYCT DFLLCASSSG AVLPVRTQDP
460 470 480 490 500
FLPYPPRSCP QGQAASSATT RDPAQRVVLV LRERWHFCRD GRVLLGSRAL
510 520 530 540
RERHLGLMGY QLLPLPFEEL ESQRGLPQLK SYLRQKLQAL GLRWGPEGG
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketG3V1R6 | G3V1R6_HUMAN | Fas-activated serine/threonine kina... | FASTK | 465 | Annotation score: |
Sequence cautioni
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_042200 | 424 | V → L in a lung adenocarcinoma sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs758691941Ensembl. | 1 | |
Natural variantiVAR_021970 | 436 | A → V. Corresponds to variant dbSNP:rs2288648Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_058246 | 1 – 34 | Missing in isoform 4. 1 PublicationAdd BLAST | 34 | |
Alternative sequenceiVSP_042746 | 28 | W → C in isoform 2. 1 Publication | 1 | |
Alternative sequenceiVSP_042747 | 29 – 169 | Missing in isoform 2. 1 PublicationAdd BLAST | 141 | |
Alternative sequenceiVSP_044811 | 202 – 228 | Missing in isoform 3. 1 PublicationAdd BLAST | 27 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X86779 mRNA Translation: CAA60448.1 AK292232 mRNA Translation: BAF84921.1 AB209783 mRNA Translation: BAD93020.1 Different initiation. AC010973 Genomic DNA No translation available. CH471173 Genomic DNA Translation: EAW54039.1 CH471173 Genomic DNA Translation: EAW54043.1 BC011770 mRNA Translation: AAH11770.1 BC039026 mRNA Translation: AAH39026.1 |
CCDSi | CCDS59088.1 [Q14296-3] CCDS5918.1 [Q14296-1] CCDS5919.1 [Q14296-2] |
PIRi | I37386 |
RefSeqi | NP_001245390.1, NM_001258461.1 [Q14296-3] NP_006703.1, NM_006712.4 [Q14296-1] NP_148936.2, NM_033015.3 [Q14296-2] XP_005249989.1, XM_005249932.1 [Q14296-4] XP_005249990.1, XM_005249933.3 [Q14296-4] |
Genome annotation databases
Ensembli | ENST00000297532; ENSP00000297532; ENSG00000164896 ENST00000353841; ENSP00000324817; ENSG00000164896 [Q14296-2] ENST00000482571; ENSP00000418516; ENSG00000164896 [Q14296-3] |
GeneIDi | 10922 |
KEGGi | hsa:10922 |
MANE-Selecti | ENST00000297532.11; ENSP00000297532.6; NM_006712.5; NP_006703.1 |
UCSCi | uc003wix.3, human [Q14296-1] |
Keywords - Coding sequence diversityi
Alternative initiation, Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X86779 mRNA Translation: CAA60448.1 AK292232 mRNA Translation: BAF84921.1 AB209783 mRNA Translation: BAD93020.1 Different initiation. AC010973 Genomic DNA No translation available. CH471173 Genomic DNA Translation: EAW54039.1 CH471173 Genomic DNA Translation: EAW54043.1 BC011770 mRNA Translation: AAH11770.1 BC039026 mRNA Translation: AAH39026.1 |
CCDSi | CCDS59088.1 [Q14296-3] CCDS5918.1 [Q14296-1] CCDS5919.1 [Q14296-2] |
PIRi | I37386 |
RefSeqi | NP_001245390.1, NM_001258461.1 [Q14296-3] NP_006703.1, NM_006712.4 [Q14296-1] NP_148936.2, NM_033015.3 [Q14296-2] XP_005249989.1, XM_005249932.1 [Q14296-4] XP_005249990.1, XM_005249933.3 [Q14296-4] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 116126, 14 interactors |
IntActi | Q14296, 13 interactors |
STRINGi | 9606.ENSP00000297532 |
PTM databases
GlyGeni | Q14296, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q14296 |
PhosphoSitePlusi | Q14296 |
Genetic variation databases
BioMutai | FASTK |
DMDMi | 13124202 |
Proteomic databases
jPOSTi | Q14296 |
MassIVEi | Q14296 |
MaxQBi | Q14296 |
PaxDbi | Q14296 |
PeptideAtlasi | Q14296 |
PRIDEi | Q14296 |
ProteomicsDBi | 28667 59957 [Q14296-1] 59958 [Q14296-2] |
Protocols and materials databases
Antibodypediai | 32964, 288 antibodies from 25 providers |
DNASUi | 10922 |
Genome annotation databases
Ensembli | ENST00000297532; ENSP00000297532; ENSG00000164896 ENST00000353841; ENSP00000324817; ENSG00000164896 [Q14296-2] ENST00000482571; ENSP00000418516; ENSG00000164896 [Q14296-3] |
GeneIDi | 10922 |
KEGGi | hsa:10922 |
MANE-Selecti | ENST00000297532.11; ENSP00000297532.6; NM_006712.5; NP_006703.1 |
UCSCi | uc003wix.3, human [Q14296-1] |
Organism-specific databases
CTDi | 10922 |
DisGeNETi | 10922 |
GeneCardsi | FASTK |
HGNCi | HGNC:24676, FASTK |
HPAi | ENSG00000164896, Low tissue specificity |
MIMi | 606965, gene |
neXtProti | NX_Q14296 |
OpenTargetsi | ENSG00000164896 |
PharmGKBi | PA142671775 |
VEuPathDBi | HostDB:ENSG00000164896 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QXK7, Eukaryota |
GeneTreei | ENSGT01030000234607 |
HOGENOMi | CLU_046861_0_0_1 |
InParanoidi | Q14296 |
OMAi | KVRTHHY |
PhylomeDBi | Q14296 |
TreeFami | TF331796 |
Enzyme and pathway databases
BRENDAi | 2.7.11.8, 2681 |
PathwayCommonsi | Q14296 |
SignaLinki | Q14296 |
Miscellaneous databases
BioGRID-ORCSi | 10922, 15 hits in 1075 CRISPR screens |
ChiTaRSi | FASTK, human |
GeneWikii | FASTK |
GenomeRNAii | 10922 |
Pharosi | Q14296, Tbio |
PROi | PR:Q14296 |
RNActi | Q14296, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000164896, Expressed in apex of heart and 237 other tissues |
ExpressionAtlasi | Q14296, baseline and differential |
Genevisiblei | Q14296, HS |
Family and domain databases
InterProi | View protein in InterPro IPR013579, FAST_2 IPR010622, FAST_Leu-rich IPR013584, RAP IPR015715, Ser/Thr_kinase_FAST |
PANTHERi | PTHR21228:SF4, PTHR21228:SF4, 1 hit |
Pfami | View protein in Pfam PF06743, FAST_1, 1 hit PF08368, FAST_2, 1 hit PF08373, RAP, 1 hit |
SMARTi | View protein in SMART SM00952, RAP, 1 hit |
PROSITEi | View protein in PROSITE PS51286, RAP, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | FASTK_HUMAN | |
Accessioni | Q14296Primary (citable) accession number: Q14296 Secondary accession number(s): A8K867 Q8IVA0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 21, 2001 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 177 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human and mouse protein kinases
Human and mouse protein kinases: classification and index - Human chromosome 7
Human chromosome 7: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families