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Protein

Src substrate cortactin

Gene

CTTN

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Contributes to the organization of the actin cytoskeleton and cell shape (PubMed:21296879). Plays a role in the formation of lamellipodia and in cell migration. Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones (By similarity). Through its interaction with CTTNBP2, involved in the regulation of neuronal spine density (By similarity). Plays a role in the invasiveness of cancer cells, and the formation of metastases (PubMed:16636290). Plays a role in focal adhesion assembly and turnover (By similarity). In complex with ABL1 and MYLK regulates cortical actin-based cytoskeletal rearrangement critical to sphingosine 1-phosphate (S1P)-mediated endothelial cell (EC) barrier enhancement (PubMed:20861316). Plays a role in intracellular protein transport and endocytosis, and in modulating the levels of potassium channels present at the cell membrane (PubMed:17959782). Plays a role in receptor-mediated endocytosis via clathrin-coated pits (By similarity). Required for stabilization of KCNH1 channels at the cell membrane (PubMed:23144454).By similarity4 Publications

GO - Molecular functioni

  • cadherin binding Source: BHF-UCL
  • profilin binding Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processEndocytosis

Enzyme and pathway databases

ReactomeiR-HSA-5627123 RHO GTPases activate PAKs
R-HSA-8856828 Clathrin-mediated endocytosis
SIGNORiQ14247

Protein family/group databases

MoonDBiQ14247 Predicted

Names & Taxonomyi

Protein namesi
Recommended name:
Src substrate cortactin
Alternative name(s):
Amplaxin
Oncogene EMS1
Gene namesi
Name:CTTN
Synonyms:EMS1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000085733.15
HGNCiHGNC:3338 CTTN
MIMi164765 gene
neXtProtiNX_Q14247

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Coated pit, Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi2017
OpenTargetsiENSG00000085733
PharmGKBiPA27775

Polymorphism and mutation databases

BioMutaiCTTN
DMDMi215273892

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000721891 – 550Src substrate cortactinAdd BLAST550

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei87N6-acetyllysineCombined sources1
Modified residuei113PhosphoserineBy similarity1
Modified residuei119Omega-N-methylarginineBy similarity1
Modified residuei124N6-acetyllysineBy similarity1
Modified residuei144N6-acetyllysine; alternateBy similarity1
Cross-linki144Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateCombined sources
Cross-linki144Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Modified residuei150PhosphoserineCombined sources1
Modified residuei161N6-acetyllysineBy similarity1
Modified residuei181N6-acetyllysine; alternateBy similarity1
Cross-linki181Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateCombined sources
Cross-linki181Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Modified residuei198N6-acetyllysineCombined sources1
Cross-linki218Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)Combined sources
Modified residuei235N6-acetyllysineCombined sources1
Modified residuei261PhosphoserineCombined sources1
Modified residuei272N6-acetyllysineCombined sources1
Modified residuei295N6-acetyllysine; alternateBy similarity1
Cross-linki295Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Modified residuei304N6-acetyllysineCombined sources1
Modified residuei309N6-acetyllysineCombined sources1
Modified residuei346N6-acetyllysineBy similarity1
Modified residuei399PhosphothreonineCombined sources1
Modified residuei401PhosphothreonineCombined sources1
Modified residuei405PhosphoserineCombined sources1
Modified residuei411PhosphothreonineCombined sources1
Modified residuei417PhosphoserineCombined sources1
Modified residuei418PhosphoserineCombined sources1
Modified residuei421Phosphotyrosine; by SRCCombined sources1
Modified residuei426PhosphoserineCombined sources1
Modified residuei438PhosphoserineCombined sources1
Modified residuei446PhosphotyrosineCombined sources1
Modified residuei447PhosphoserineCombined sources1
Modified residuei453PhosphotyrosineCombined sources1
Modified residuei470Phosphotyrosine; by FAK1By similarity1
Modified residuei486Phosphotyrosine; by SRC2 Publications1
Modified residuei489Phosphotyrosine; by SRCBy similarity1

Post-translational modificationi

Phosphorylated by PKN2 at both serine and threonine residues in a GTP-bound Rac1-dependent manner in hyaluronan-induced astrocytes and hence down-regulated CTTN ability to associates with filamentous actin (By similarity). Phosphorylated on tyrosine residues in response to CHRM1 activation (By similarity). Phosphorylated by PTK2/FAK1 in response to cell adhesion (By similarity). Phosphorylated by FER. Tyrosine phosphorylation in transformed cells may contribute to cellular growth regulation and transformation. Phosphorylated in response to FGR activation. Phosphorylation by SRC promotes MYLK binding.By similarity3 Publications

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ14247
MaxQBiQ14247
PeptideAtlasiQ14247
PRIDEiQ14247
ProteomicsDBi59947
59948 [Q14247-2]
59949 [Q14247-3]

2D gel databases

OGPiQ14247

PTM databases

iPTMnetiQ14247
PhosphoSitePlusiQ14247
SwissPalmiQ14247

Expressioni

Gene expression databases

BgeeiENSG00000085733
CleanExiHS_CTTN
ExpressionAtlasiQ14247 baseline and differential
GenevisibleiQ14247 HS

Organism-specific databases

HPAiCAB011235
HPA057242

Interactioni

Subunit structurei

Interacts with SHANK2 and SHANK3 (via its SH3 domain). Also interacts with FGD1. Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. Interacts with ABL2 (By similarity). Interacts with KCNA2 (via non-phosphorylated C-terminus) (PubMed:12151401). Interacts with PLXDC2 and SRCIN1. Interacts with SAMSN1 (via SH3 domain). Interacts (via SH3 domain) with ASAP1 (via Pro-rich region). Interacts (via SH3 domain) with DNM2 (By similarity). Interacts with ACTN1 (By similarity). Interacts with FER (PubMed:9722593). Forms a complex made of ABL1 and MYLK. Interacts with CTTNBP2NL; this interaction may target CTTN to stress fibers. Interacts with CTTNBP2; this interaction may target CTTN at the cell cortex or dendritic spines. Interacts with KCNH1 (PubMed:23144454).By similarity9 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • cadherin binding Source: BHF-UCL
  • profilin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi108332, 127 interactors
CORUMiQ14247
DIPiDIP-33190N
IntActiQ14247, 71 interactors
MINTiQ14247

Structurei

Secondary structure

1550
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi497 – 499Combined sources3
Beta strandi506 – 510Combined sources5
Beta strandi518 – 523Combined sources6
Beta strandi526 – 534Combined sources9
Beta strandi537 – 542Combined sources6
Helixi543 – 545Combined sources3
Beta strandi546 – 548Combined sources3

3D structure databases

ProteinModelPortaliQ14247
SMRiQ14247
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ14247

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati80 – 116Cortactin 1Add BLAST37
Repeati117 – 153Cortactin 2Add BLAST37
Repeati154 – 190Cortactin 3Add BLAST37
Repeati191 – 227Cortactin 4Add BLAST37
Repeati228 – 264Cortactin 5Add BLAST37
Repeati265 – 301Cortactin 6Add BLAST37
Repeati302 – 324Cortactin 7; truncatedAdd BLAST23
Domaini492 – 550SH3PROSITE-ProRule annotationAdd BLAST59

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili348 – 401Sequence analysisAdd BLAST54

Domaini

The SH3 motif may mediate binding to the cytoskeleton.Curated

Keywords - Domaini

Coiled coil, Repeat, SH3 domain

Phylogenomic databases

GeneTreeiENSGT00530000062953
HOGENOMiHOG000006523
HOVERGENiHBG005994
InParanoidiQ14247
KOiK06106
OMAiWDHIEKV
OrthoDBiEOG091G0CPX
PhylomeDBiQ14247
TreeFamiTF318935

Family and domain databases

CDDicd11959 SH3_Cortactin, 1 hit
InterProiView protein in InterPro
IPR015503 Cortactin
IPR035716 Cortactin_SH3
IPR003134 Hs1_Cortactin
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PANTHERiPTHR10829:SF15 PTHR10829:SF15, 3 hits
PfamiView protein in Pfam
PF02218 HS1_rep, 7 hits
PF14604 SH3_9, 1 hit
PRINTSiPR00452 SH3DOMAIN
SMARTiView protein in SMART
SM00326 SH3, 1 hit
SUPFAMiSSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS51090 CORTACTIN, 7 hits
PS50002 SH3, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q14247-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MWKASAGHAV SIAQDDAGAD DWETDPDFVN DVSEKEQRWG AKTVQGSGHQ
60 70 80 90 100
EHINIHKLRE NVFQEHQTLK EKELETGPKA SHGYGGKFGV EQDRMDKSAV
110 120 130 140 150
GHEYQSKLSK HCSQVDSVRG FGGKFGVQMD RVDQSAVGFE YQGKTEKHAS
160 170 180 190 200
QKDYSSGFGG KYGVQADRVD KSAVGFDYQG KTEKHESQRD YSKGFGGKYG
210 220 230 240 250
IDKDKVDKSA VGFEYQGKTE KHESQKDYVK GFGGKFGVQT DRQDKCALGW
260 270 280 290 300
DHQEKLQLHE SQKDYKTGFG GKFGVQSERQ DSAAVGFDYK EKLAKHESQQ
310 320 330 340 350
DYSKGFGGKY GVQKDRMDKN ASTFEDVTQV SSAYQKTVPV EAVTSKTSNI
360 370 380 390 400
RANFENLAKE KEQEDRRKAE AERAQRMAKE RQEQEEARRK LEEQARAKTQ
410 420 430 440 450
TPPVSPAPQP TEERLPSSPV YEDAASFKAE LSYRGPVSGT EPEPVYSMEA
460 470 480 490 500
ADYREASSQQ GLAYATEAVY ESAEAPGHYP AEDSTYDEYE NDLGITAVAL
510 520 530 540 550
YDYQAAGDDE ISFDPDDIIT NIEMIDDGWW RGVCKGRYGL FPANYVELRQ
Length:550
Mass (Da):61,586
Last modified:November 25, 2008 - v2
Checksum:i7799326C2B4383BB
GO
Isoform 2 (identifier: Q14247-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     264-300: Missing.
     538-550: YGLFPANYVELRQ → FRELAFSCVR...CLGTWVPECP

Note: No experimental confirmation available.
Show »
Length:634
Mass (Da):70,959
Checksum:i9F36B5AED4992281
GO
Isoform 3 (identifier: Q14247-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     264-300: Missing.

Show »
Length:513
Mass (Da):57,467
Checksum:i333C09D488528C40
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti495I → Y in AAA58455 (PubMed:1532244).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_043120264 – 300Missing in isoform 2 and isoform 3. 2 PublicationsAdd BLAST37
Alternative sequenceiVSP_043121538 – 550YGLFP…VELRQ → FRELAFSCVRVALVPIKCSR DLPGQARGLRSALWRVGRKD CPRRGASSRVSLLGRRGLGL MEVNPELSHPEHRSCHVRWE ICLCHTVTARRIRKLISFLR SREAGPVPSCSQVGGVSFQK VTWKCLGTWVPECP in isoform 2. 1 PublicationAdd BLAST13

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M98343 mRNA Translation: AAA58455.1
AK291097 mRNA Translation: BAF83786.1
AP000487 Genomic DNA No translation available.
CH471076 Genomic DNA Translation: EAW74766.1
CH471076 Genomic DNA Translation: EAW74768.1
BC008799 mRNA Translation: AAH08799.1
BC033889 mRNA Translation: AAH33889.1
CCDSiCCDS41680.1 [Q14247-1]
CCDS53676.1 [Q14247-2]
CCDS8197.1 [Q14247-3]
PIRiA48063
RefSeqiNP_001171669.1, NM_001184740.1 [Q14247-2]
NP_005222.2, NM_005231.3 [Q14247-1]
NP_612632.1, NM_138565.2 [Q14247-3]
UniGeneiHs.596164

Genome annotation databases

EnsembliENST00000301843; ENSP00000301843; ENSG00000085733 [Q14247-1]
ENST00000346329; ENSP00000317189; ENSG00000085733 [Q14247-3]
ENST00000376561; ENSP00000365745; ENSG00000085733 [Q14247-2]
GeneIDi2017
KEGGihsa:2017
UCSCiuc001opu.4 human [Q14247-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSRC8_HUMAN
AccessioniPrimary (citable) accession number: Q14247
Secondary accession number(s): Q8N707, Q96H99
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 25, 2008
Last modified: June 20, 2018
This is version 190 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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