UniProtKB - Q14242 (SELPL_HUMAN)
P-selectin glycoprotein ligand 1
SELPLG
Functioni
A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.
2 Publications(Microbial infection) Acts as a receptor for enterovirus 71.
1 PublicationGO - Molecular functioni
- signaling receptor binding Source: ProtInc
- virus receptor activity Source: UniProtKB-KW
GO - Biological processi
- cell adhesion Source: ProtInc
- cellular response to interleukin-6 Source: MGI
- hemopoiesis Source: GO_Central
- leukocyte adhesive activation Source: UniProtKB
- leukocyte migration Source: CAFA
- leukocyte tethering or rolling Source: CAFA
Keywordsi
Molecular function | Host cell receptor for virus entry, Receptor |
Biological process | Cell adhesion, Host-virus interaction |
Ligand | Sialic acid |
Enzyme and pathway databases
PathwayCommonsi | Q14242 |
Reactomei | R-HSA-202733, Cell surface interactions at the vascular wall |
SignaLinki | Q14242 |
SIGNORi | Q14242 |
Names & Taxonomyi
Protein namesi | Recommended name: P-selectin glycoprotein ligand 1Short name: PSGL-1 Alternative name(s): Selectin P ligand CD_antigen: CD162 |
Gene namesi | Name:SELPLG |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:10722, SELPLG |
MIMi | 600738, gene |
neXtProti | NX_Q14242 |
VEuPathDBi | HostDB:ENSG00000110876 |
Subcellular locationi
Other locations
Plasma Membrane
- integral component of plasma membrane Source: ProtInc
- plasma membrane Source: UniProtKB
- plasma membrane raft Source: CAFA
Other locations
- integral component of membrane Source: ProtInc
- membrane Source: ProtInc
- uropod Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 18 – 320 | ExtracellularSequence analysisAdd BLAST | 303 | |
Transmembranei | 321 – 341 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 342 – 412 | CytoplasmicSequence analysisAdd BLAST | 71 |
Keywords - Cellular componenti
MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 44 | T → A: No effect on L-selectin binding nor neutrophil rolling. 1 Publication | 1 | |
Mutagenesisi | 46 – 52 | YEYLDYD → FEFLDFE: No sulfation. Almost complete loss of P-selectin binding. No effect on E-selectin binding. 1 Publication | 7 | |
Mutagenesisi | 46 – 51 | YEYLDY → FEFLDF: No sulfation. Almost complete loss of P-selectin binding. No effect on E-selectin binding. | 6 | |
Mutagenesisi | 46 | Y → F: Binding L-selectin reduced by 20%, neutrophil recruitment reduced by 30%, and lymphocyte rolling reduced by 32%; when associated with F-48. Binding L-selectin reduced by 86%, neutrophil recruitment reduced by 75%, and lymphocyte rolling reduced by 69%; when associated with F-51. Binding L-selectin reduced by 89%, and neutrophil recruitment reduced by 90%; when associated with F-48 and F-51. Binding of L-selectin reduced by 91%; when associated with F-48; F-51 and A-57. 1 Publication | 1 | |
Mutagenesisi | 48 | Y → F: Binding L-selectin reduced by 20%, neutrophil recruitment reduced by 30%, and lymphocyte rolling reduced by 32%; when associated with F-46. Binding L-lectin reduced by 31%, neutrophil recruitment reduced by 52%, and lymphocyte rolling reduced by 52%; when associated with F-51. Binding L-selectin reduced by 89%, and neutrophil recruitment reduced by 90%; when associated with F-46 and F-51. Binding of L-selectin reduced by 91%; when associated with F-46; F-51 and A-57. 2 Publications | 1 | |
Mutagenesisi | 51 | Y → F: Binding L-selectin reduced by 86%, neutrophil recruitment reduced by 75% and, lymphocyte rolling reduced by 69%; when associated with F-46. Binding L-selectin reduced by 31%, neutrophil recruitment reduced by 52%, and lymphocyte rolling reduced by 52%; when associated with F-48; Binding L-selectin reduced by 89%, and neutrophil recruitment reduced by 90%; when associated with F-46 and F-48. Binding of L-selectin reduced by 91%; when associated with F-46; F-48 and A-57. 2 Publications | 1 | |
Mutagenesisi | 57 | T → A: No E- nor P-selectin binding, and very little neutrophil rolling. Binding of L-selectin reduced by 91%; when associated with F-46; F-48 and F-51. 2 Publications | 1 | |
Mutagenesisi | 320 | C → A or S: No dimer formation. No effect on P-selectin binding. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 6404 |
OpenTargetsi | ENSG00000110876 |
PharmGKBi | PA35644 |
Miscellaneous databases
Pharosi | Q14242, Tbio |
Chemistry databases
ChEMBLi | CHEMBL4183 |
DrugBanki | DB16698, Neihulizumab |
Genetic variation databases
BioMutai | SELPLG |
DMDMi | 2498904 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 17 | Sequence analysisAdd BLAST | 17 | |
PropeptideiPRO_0000022302 | 18 – 41 | Add BLAST | 24 | |
ChainiPRO_0000022303 | 42 – 412 | P-selectin glycoprotein ligand 1Add BLAST | 371 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 42 | Pyrrolidone carboxylic acid1 Publication | 1 | |
Modified residuei | 46 | Sulfotyrosine1 Publication | 1 | |
Modified residuei | 48 | Sulfotyrosine1 Publication | 1 | |
Modified residuei | 51 | Sulfotyrosine1 Publication | 1 | |
Glycosylationi | 57 | O-linked (GalNAc...) threonine1 Publication | 1 | |
Glycosylationi | 65 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 111 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 302 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 320 | Interchain1 Publication | ||
Modified residuei | 406 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 409 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Phosphoprotein, Pyrrolidone carboxylic acid, SulfationProteomic databases
EPDi | Q14242 |
jPOSTi | Q14242 |
MassIVEi | Q14242 |
MaxQBi | Q14242 |
PaxDbi | Q14242 |
PeptideAtlasi | Q14242 |
PRIDEi | Q14242 |
ProteomicsDBi | 59939 [Q14242-1] |
PTM databases
GlyConnecti | 520, 6 O-Linked glycans |
GlyGeni | Q14242, 5 sites, 9 O-linked glycans (1 site) |
iPTMneti | Q14242 |
PhosphoSitePlusi | Q14242 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000110876, Expressed in blood and 222 other tissues |
ExpressionAtlasi | Q14242, baseline and differential |
Genevisiblei | Q14242, HS |
Organism-specific databases
HPAi | ENSG00000110876, Tissue enhanced (lymphoid) |
Interactioni
Subunit structurei
Homodimer; disulfide-linked. Interaction with P-, E- and L-selectins, through their lectin/EGF domains, is required for promoting recruitment and rolling of leukocytes. These interactions require sialyl Lewis X glycan modification but there is a differing dependence for tyrosine sulfations. Sulfation on Tyr-51 of PSGL1 is most important for high affinity L-selectin/SELL binding while P-selectin/SELP requires sulfation on Tyr-48. E-selectin/SELE binds with much lower affinity and requires the sLe(x) epitope, but apparently not tyrosine sulfation. Dimerization appears not to be required for P-selectin/SELP binding.
Interacts with SNX20.
Interacts with MSN and SYK; mediates the activation of SYK by SELPLG.
Interacts with HAVCR1 (PubMed:24703780).
12 Publications(Microbial infection) Interacts with enterovirus 71 capsid proteins.
1 Publication(Microbial infection) Interacts with Staphylococcus aureus proteins SSL5 and SSL11; these interactions prevent SELPLG-mediated neutrophil rolling.
2 PublicationsBinary interactionsi
GO - Molecular functioni
- signaling receptor binding Source: ProtInc
Protein-protein interaction databases
BioGRIDi | 112304, 7 interactors |
DIPi | DIP-37668N |
IntActi | Q14242, 7 interactors |
STRINGi | 9606.ENSP00000228463 |
Chemistry databases
BindingDBi | Q14242 |
Miscellaneous databases
RNActi | Q14242, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q14242 |
SMRi | Q14242 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q14242 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 122 – 131 | 1 | 10 | |
Repeati | 132 – 141 | 2 | 10 | |
Repeati | 142 – 151 | 3 | 10 | |
Repeati | 162 – 171 | 4 | 10 | |
Repeati | 182 – 191 | 5 | 10 | |
Repeati | 192 – 201 | 6 | 10 | |
Repeati | 202 – 211 | 7 | 10 | |
Repeati | 212 – 221 | 8 | 10 | |
Repeati | 222 – 231 | 9 | 10 | |
Repeati | 232 – 241 | 10 | 10 | |
Repeati | 242 – 251 | 11 | 10 | |
Repeati | 252 – 261 | 12 | 10 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 56 – 95 | DisorderedSequence analysisAdd BLAST | 40 | |
Regioni | 122 – 261 | 12 X 10 AA tandem repeatsAdd BLAST | 140 | |
Regioni | 125 – 146 | DisorderedSequence analysisAdd BLAST | 22 | |
Regioni | 166 – 252 | DisorderedSequence analysisAdd BLAST | 87 | |
Regioni | 374 – 412 | DisorderedSequence analysisAdd BLAST | 39 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 62 – 87 | Polar residuesSequence analysisAdd BLAST | 26 | |
Compositional biasi | 392 – 406 | Basic and acidic residuesSequence analysisAdd BLAST | 15 |
Keywords - Domaini
Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502S8ZU, Eukaryota |
GeneTreei | ENSGT00440000039754 |
HOGENOMi | CLU_061579_0_0_1 |
InParanoidi | Q14242 |
OMAi | HTYPVRN |
OrthoDBi | 1055530at2759 |
PhylomeDBi | Q14242 |
TreeFami | TF337792 |
Family and domain databases
IDEALi | IID00352 |
InterProi | View protein in InterPro IPR026195, PSGL-1 |
PANTHERi | PTHR17384, PTHR17384, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MPLQLLLLLI LLGPGNSLQL WDTWADEAEK ALGPLLARDR RQATEYEYLD
60 70 80 90 100
YDFLPETEPP EMLRNSTDTT PLTGPGTPES TTVEPAARRS TGLDAGGAVT
110 120 130 140 150
ELTTELANMG NLSTDSAAME IQTTQPAATE AQTTQPVPTE AQTTPLAATE
160 170 180 190 200
AQTTRLTATE AQTTPLAATE AQTTPPAATE AQTTQPTGLE AQTTAPAAME
210 220 230 240 250
AQTTAPAAME AQTTPPAAME AQTTQTTAME AQTTAPEATE AQTTQPTATE
260 270 280 290 300
AQTTPLAAME ALSTEPSATE ALSMEPTTKR GLFIPFSVSS VTHKGIPMAA
310 320 330 340 350
SNLSVNYPVG APDHISVKQC LLAILILALV ATIFFVCTVV LAVRLSRKGH
360 370 380 390 400
MYPVRNYSPT EMVCISSLLP DGGEGPSATA NGGLSKAKSP GLTPEPREDR
410
EGDDLTLHSF LP
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0C4DFY0 | A0A0C4DFY0_HUMAN | P-selectin glycoprotein ligand 1 | SELPLG | 402 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 23 – 39 | Missing in BAC05283 (PubMed:14702039).CuratedAdd BLAST | 17 | |
Sequence conflicti | 50 | D → E in BAC05283 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 219 | M → T in BAC05283 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 222 | Q → R in BAH12863 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 396 | P → A in BAC05283 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_019156 | 62 | M → I2 PublicationsCorresponds to variant dbSNP:rs2228315Ensembl. | 1 | |
Natural variantiVAR_005611 | 132 – 141 | Missing in short form; not an alternative splicing. 3 PublicationsCorresponds to variant dbSNP:rs63748999Ensembl. | 10 | |
Natural variantiVAR_019157 | 246 | P → S1 PublicationCorresponds to variant dbSNP:rs8179142Ensembl. | 1 | |
Natural variantiVAR_076761 | 249 | T → M1 PublicationCorresponds to variant dbSNP:rs756234416Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_044827 | 1 | M → MAVGASGLEGDKMAGAM in isoform 2. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U25956 Genomic DNA Translation: AAA74577.1 U02297 mRNA Translation: AAC50061.1 AK098315 mRNA Translation: BAC05283.1 AK290395 mRNA Translation: BAF83084.1 AK298738 mRNA Translation: BAG60885.1 AK298742 mRNA Translation: BAH12863.1 AY331789 Genomic DNA Translation: AAP81163.1 AC007569 Genomic DNA No translation available. AC008119 Genomic DNA No translation available. BC029782 mRNA Translation: AAH29782.1 |
CCDSi | CCDS31895.2 [Q14242-1] CCDS55881.1 [Q14242-2] |
PIRi | A57468 |
RefSeqi | NP_001193538.1, NM_001206609.1 [Q14242-2] NP_002997.2, NM_003006.4 [Q14242-1] |
Genome annotation databases
Ensembli | ENST00000228463.6; ENSP00000228463.6; ENSG00000110876.10 [Q14242-2] ENST00000550948.2; ENSP00000447752.1; ENSG00000110876.10 |
GeneIDi | 6404 |
KEGGi | hsa:6404 |
MANE-Selecti | ENST00000550948.2; ENSP00000447752.1; NM_003006.4; NP_002997.2 |
UCSCi | uc001tni.4, human [Q14242-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
Wikipedia P-selectin glycoprotein ligand 1 entry |
SeattleSNPs |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U25956 Genomic DNA Translation: AAA74577.1 U02297 mRNA Translation: AAC50061.1 AK098315 mRNA Translation: BAC05283.1 AK290395 mRNA Translation: BAF83084.1 AK298738 mRNA Translation: BAG60885.1 AK298742 mRNA Translation: BAH12863.1 AY331789 Genomic DNA Translation: AAP81163.1 AC007569 Genomic DNA No translation available. AC008119 Genomic DNA No translation available. BC029782 mRNA Translation: AAH29782.1 |
CCDSi | CCDS31895.2 [Q14242-1] CCDS55881.1 [Q14242-2] |
PIRi | A57468 |
RefSeqi | NP_001193538.1, NM_001206609.1 [Q14242-2] NP_002997.2, NM_003006.4 [Q14242-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1G1S | X-ray | 1.90 | C/D | 42-60 | [»] | |
AlphaFoldDBi | Q14242 | |||||
SMRi | Q14242 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 112304, 7 interactors |
DIPi | DIP-37668N |
IntActi | Q14242, 7 interactors |
STRINGi | 9606.ENSP00000228463 |
Chemistry databases
BindingDBi | Q14242 |
ChEMBLi | CHEMBL4183 |
DrugBanki | DB16698, Neihulizumab |
PTM databases
GlyConnecti | 520, 6 O-Linked glycans |
GlyGeni | Q14242, 5 sites, 9 O-linked glycans (1 site) |
iPTMneti | Q14242 |
PhosphoSitePlusi | Q14242 |
Genetic variation databases
BioMutai | SELPLG |
DMDMi | 2498904 |
Proteomic databases
EPDi | Q14242 |
jPOSTi | Q14242 |
MassIVEi | Q14242 |
MaxQBi | Q14242 |
PaxDbi | Q14242 |
PeptideAtlasi | Q14242 |
PRIDEi | Q14242 |
ProteomicsDBi | 59939 [Q14242-1] |
Protocols and materials databases
ABCDi | Q14242, 1 sequenced antibody |
Antibodypediai | 3720, 1131 antibodies from 40 providers |
DNASUi | 6404 |
Genome annotation databases
Ensembli | ENST00000228463.6; ENSP00000228463.6; ENSG00000110876.10 [Q14242-2] ENST00000550948.2; ENSP00000447752.1; ENSG00000110876.10 |
GeneIDi | 6404 |
KEGGi | hsa:6404 |
MANE-Selecti | ENST00000550948.2; ENSP00000447752.1; NM_003006.4; NP_002997.2 |
UCSCi | uc001tni.4, human [Q14242-1] |
Organism-specific databases
CTDi | 6404 |
DisGeNETi | 6404 |
GeneCardsi | SELPLG |
HGNCi | HGNC:10722, SELPLG |
HPAi | ENSG00000110876, Tissue enhanced (lymphoid) |
MIMi | 600738, gene |
neXtProti | NX_Q14242 |
OpenTargetsi | ENSG00000110876 |
PharmGKBi | PA35644 |
VEuPathDBi | HostDB:ENSG00000110876 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502S8ZU, Eukaryota |
GeneTreei | ENSGT00440000039754 |
HOGENOMi | CLU_061579_0_0_1 |
InParanoidi | Q14242 |
OMAi | HTYPVRN |
OrthoDBi | 1055530at2759 |
PhylomeDBi | Q14242 |
TreeFami | TF337792 |
Enzyme and pathway databases
PathwayCommonsi | Q14242 |
Reactomei | R-HSA-202733, Cell surface interactions at the vascular wall |
SignaLinki | Q14242 |
SIGNORi | Q14242 |
Miscellaneous databases
BioGRID-ORCSi | 6404, 15 hits in 1082 CRISPR screens |
ChiTaRSi | SELPLG, human |
EvolutionaryTracei | Q14242 |
GeneWikii | P-selectin_glycoprotein_ligand-1 |
GenomeRNAii | 6404 |
Pharosi | Q14242, Tbio |
PROi | PR:Q14242 |
RNActi | Q14242, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000110876, Expressed in blood and 222 other tissues |
ExpressionAtlasi | Q14242, baseline and differential |
Genevisiblei | Q14242, HS |
Family and domain databases
IDEALi | IID00352 |
InterProi | View protein in InterPro IPR026195, PSGL-1 |
PANTHERi | PTHR17384, PTHR17384, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SELPL_HUMAN | |
Accessioni | Q14242Primary (citable) accession number: Q14242 Secondary accession number(s): A8K2Y0 Q8N7J7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 189 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human cell differentiation molecules
CD nomenclature of surface proteins of human leucocytes and list of entries - Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references