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Protein

Transcription factor E2F2

Gene

E2F2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F2 binds specifically to RB1 in a cell-cycle dependent manner.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi107 – 196Sequence analysisAdd BLAST90

GO - Molecular functioni

  • core promoter binding Source: UniProtKB
  • DNA binding Source: ProtInc
  • DNA binding transcription factor activity Source: ProtInc
  • protein dimerization activity Source: InterPro
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: NTNU_SB
  • sequence-specific DNA binding Source: NTNU_SB
  • transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: NTNU_SB
  • transcription factor binding Source: UniProtKB

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • intrinsic apoptotic signaling pathway by p53 class mediator Source: Ensembl
  • lens fiber cell apoptotic process Source: Ensembl
  • negative regulation of sprouting angiogenesis Source: BHF-UCL
  • positive regulation of transcription by RNA polymerase II Source: NTNU_SB
  • regulation of cell cycle Source: Ensembl
  • transcription initiation from RNA polymerase II promoter Source: ProtInc

Keywordsi

Molecular functionActivator, DNA-binding
Biological processCell cycle, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-2559580 Oxidative Stress Induced Senescence
R-HSA-2559585 Oncogene Induced Senescence
R-HSA-68689 CDC6 association with the ORC:origin complex
R-HSA-69231 Cyclin D associated events in G1
SignaLinkiQ14209
SIGNORiQ14209

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor E2F2
Short name:
E2F-2
Gene namesi
Name:E2F2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000007968.6
HGNCiHGNC:3114 E2F2
MIMi600426 gene
neXtProtiNX_Q14209

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi1870
OpenTargetsiENSG00000007968
PharmGKBiPA27572

Polymorphism and mutation databases

BioMutaiE2F2
DMDMi2494228

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002194641 – 437Transcription factor E2F2Add BLAST437

Post-translational modificationi

Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ14209
PaxDbiQ14209
PeptideAtlasiQ14209
PRIDEiQ14209
ProteomicsDBi59931

PTM databases

iPTMnetiQ14209
PhosphoSitePlusiQ14209

Expressioni

Tissue specificityi

Highest level of expression is found in placenta, low levels are found in lung. Found as well in many immortalized cell lines derived from tumor samples.

Gene expression databases

BgeeiENSG00000007968
CleanExiHS_E2F2
GenevisibleiQ14209 HS

Organism-specific databases

HPAiCAB016313

Interactioni

Subunit structurei

Component of the DRTF1/E2F transcription factor complex. Forms heterodimers with DP family members. The E2F2 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active. Viral oncoproteins, notably E1A, T-antigen and HPV E7, are capable of sequestering RB1, thus releasing the active complex. Binds EAPP.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
RB1P064003EBI-718476,EBI-491274

GO - Molecular functioni

  • protein dimerization activity Source: InterPro
  • transcription factor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi108202, 25 interactors
ComplexPortaliCPX-175 RB1-E2F2-TFDP1 transcription repressor complex
CPX-1972 E2F2-DP1 transcription factor complex
DIPiDIP-258N
ELMiQ14209
IntActiQ14209, 12 interactors
MINTiQ14209
STRINGi9606.ENSP00000355249

Structurei

Secondary structure

1437
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi422 – 424Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1N4MX-ray2.20C/D/E410-427[»]
ProteinModelPortaliQ14209
SMRiQ14209
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ14209

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni65 – 105Cyclin A/CDK2 bindingSequence analysisAdd BLAST41
Regioni155 – 176Leucine-zipperAdd BLAST22
Regioni197 – 289DimerizationSequence analysisAdd BLAST93
Regioni359 – 437TransactivationSequence analysisAdd BLAST79
Regioni410 – 427Retinoblastoma protein bindingSequence analysisAdd BLAST18

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi160 – 196DEF boxAdd BLAST37

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi360 – 363Poly-Pro4

Sequence similaritiesi

Belongs to the E2F/DP family.Curated

Phylogenomic databases

eggNOGiKOG2577 Eukaryota
ENOG410XNYI LUCA
GeneTreeiENSGT00550000074403
HOGENOMiHOG000232045
HOVERGENiHBG002227
InParanoidiQ14209
KOiK09389
OMAiNWATEVL
OrthoDBiEOG091G087U
PhylomeDBiQ14209
TreeFamiTF105566

Family and domain databases

CDDicd14660 E2F_DD, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR015633 E2F
IPR037241 E2F-DP_heterodim
IPR032198 E2F_CC-MB
IPR003316 E2F_WHTH_DNA-bd_dom
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR12081 PTHR12081, 1 hit
PfamiView protein in Pfam
PF16421 E2F_CC-MB, 1 hit
PF02319 E2F_TDP, 1 hit
SMARTiView protein in SMART
SM01372 E2F_TDP, 1 hit
SUPFAMiSSF144074 SSF144074, 1 hit
SSF46785 SSF46785, 1 hit

Sequencei

Sequence statusi: Complete.

Q14209-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLQGPRALAS AAGQTPKVVP AMSPTELWPS GLSSPQLCPA TATYYTPLYP
60 70 80 90 100
QTAPPAAAPG TCLDATPHGP EGQVVRCLPA GRLPAKRKLD LEGIGRPVVP
110 120 130 140 150
EFPTPKGKCI RVDGLPSPKT PKSPGEKTRY DTSLGLLTKK FIYLLSESED
160 170 180 190 200
GVLDLNWAAE VLDVQKRRIY DITNVLEGIQ LIRKKAKNNI QWVGRGMFED
210 220 230 240 250
PTRPGKQQQL GQELKELMNT EQALDQLIQS CSLSFKHLTE DKANKRLAYV
260 270 280 290 300
TYQDIRAVGN FKEQTVIAVK APPQTRLEVP DRTEDNLQIY LKSTQGPIEV
310 320 330 340 350
YLCPEEVQEP DSPSEEPLPS TSTLCPSPDS AQPSSSTDPS IMEPTASSVP
360 370 380 390 400
APAPTPQQAP PPPSLVPLEA TDSLLELPHP LLQQTEDQFL SPTLACSSPL
410 420 430
ISFSPSLDQD DYLWGLEAGE GISDLFDSYD LGDLLIN
Length:437
Mass (Da):47,506
Last modified:November 1, 1996 - v1
Checksum:i60541F4235507005
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_018990205G → R1 PublicationCorresponds to variant dbSNP:rs2229297Ensembl.1
Natural variantiVAR_018991226Q → H2 PublicationsCorresponds to variant dbSNP:rs2075995Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22846 mRNA Translation: AAA16890.1
AK313939 mRNA Translation: BAG36658.1
AF518877 Genomic DNA Translation: AAM54044.1
AL021154 Genomic DNA No translation available.
BC053676 mRNA Translation: AAH53676.1
CCDSiCCDS236.1
PIRiA54595
RefSeqiNP_004082.1, NM_004091.3
UniGeneiHs.194333

Genome annotation databases

EnsembliENST00000361729; ENSP00000355249; ENSG00000007968
ENST00000634683; ENSP00000489612; ENSG00000282899
GeneIDi1870
KEGGihsa:1870
UCSCiuc001bhe.3 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiE2F2_HUMAN
AccessioniPrimary (citable) accession number: Q14209
Secondary accession number(s): B2R9W1, Q7Z6H1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 20, 2018
This is version 171 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

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