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Protein

Protein NPAT

Gene

NPAT

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for progression through the G1 and S phases of the cell cycle and for S phase entry. Activates transcription of the histone H2A, histone H2B, histone H3 and histone H4 genes in conjunction with MIZF. Also positively regulates the ATM, MIZF and PRKDC promoters. Transcriptional activation may be accomplished at least in part by the recruitment of the NuA4 histone acetyltransferase (HAT) complex to target gene promoters.14 Publications

GO - Molecular functioni

  • protein C-terminus binding Source: UniProtKB
  • protein N-terminus binding Source: UniProtKB
  • transcription coactivator activity Source: UniProtKB
  • transcription corepressor activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionActivator
Biological processCell cycle, Transcription, Transcription regulation

Enzyme and pathway databases

SIGNORiQ14207

Names & Taxonomyi

Protein namesi
Recommended name:
Protein NPAT
Alternative name(s):
Nuclear protein of the ataxia telangiectasia mutated locus
Short name:
Nuclear protein of the ATM locus
p220
Gene namesi
Name:NPAT
Synonyms:CAND3, E14
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000149308.16
HGNCiHGNC:7896 NPAT
MIMi601448 gene
neXtProtiNX_Q14207

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi7V → A: Impairs activation of histone gene transcription; when associated with A-10; A-11; A-15 and A-18. 1 Publication1
Mutagenesisi10L → A: Impairs activation of histone gene transcription; when associated with A-7; A-11; A-15 and A-18. 1 Publication1
Mutagenesisi11V → A: Impairs activation of histone gene transcription; when associated with A-7; A-10; A-15 and A-18. 1 Publication1
Mutagenesisi15L → A: Impairs activation of histone gene transcription; when associated with A-7; A-10; A-11 and A-18. 1 Publication1
Mutagenesisi18E → A: Impairs activation of histone gene transcription; when associated with A-7; A-10; A-11 and A-15. 1 Publication1
Mutagenesisi27F → A: Impairs activation of histone gene transcription; when associated with A-30. 1 Publication1
Mutagenesisi30E → A: Impairs activation of histone gene transcription; when associated with A-27. 1 Publication1
Mutagenesisi331 – 333LFD → AAA: Impairs activation of histone gene transcription. Impairs interaction with BZW1, RUVBL1, RUVBL2 and TRRAP. 1 Publication3
Mutagenesisi775S → A: Impairs activation of histone gene transcription; when associated with A-779; A-1100; A-1270 and A-1350. 4 Publications1
Mutagenesisi779S → A: Impairs activation of histone gene transcription; when associated with A-775; A-1100; A-1270 and A-1350. 4 Publications1
Mutagenesisi1100S → A: Impairs activation of histone gene transcription; when associated with A-775; A-779; A-1270 and A-1350. 4 Publications1
Mutagenesisi1270T → A: Impairs activation of histone gene transcription; when associated with A-775; A-779; A-1100 and A-1350. 4 Publications1
Mutagenesisi1350T → A: Impairs activation of histone gene transcription; when associated with A-775; A-779; A-1100 and A-1270. 4 Publications1

Organism-specific databases

DisGeNETi4863
OpenTargetsiENSG00000149308
PharmGKBiPA31697

Polymorphism and mutation databases

BioMutaiNPAT
DMDMi296439285

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003181631 – 1427Protein NPATAdd BLAST1427

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei207PhosphoserineCombined sources1
Modified residuei554PhosphoserineCombined sources1
Modified residuei599PhosphoserineBy similarity1
Modified residuei775Phosphoserine; by CDK21 Publication1
Modified residuei779Phosphoserine; by CDK21 Publication1
Modified residuei1100Phosphoserine; by CDK21 Publication1
Cross-linki1116Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki1149Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei1151PhosphoserineCombined sources1
Modified residuei1200PhosphoserineCombined sources1
Modified residuei1228N6-acetyllysineCombined sources1
Modified residuei1254PhosphoserineBy similarity1
Modified residuei1270Phosphothreonine; by CDK21 Publication1
Cross-linki1280Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei1350Phosphothreonine; by CDK2Combined sources1 Publication1

Post-translational modificationi

Phosphorylated at Ser-775, Ser-779, Ser-1100, Thr-1270 and Thr-1350 by CCNE1/CDK2 at G1-S transition and until prophase, which promotes association with histone gene clusters and stimulates activation of histone transcription. Also phosphorylated by CCNA1/CDK2 in vitro.3 Publications

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ14207
MaxQBiQ14207
PaxDbiQ14207
PeptideAtlasiQ14207
PRIDEiQ14207
ProteomicsDBi59930

PTM databases

iPTMnetiQ14207
PhosphoSitePlusiQ14207

Expressioni

Tissue specificityi

Ubiquitously expressed.2 Publications

Developmental stagei

Expressed throughout the cell cycle. Expression peaks at the G1/S phase boundary and declines during S phase.2 Publications

Inductioni

By expression of E2F1, E2F2, E2F3 and E2F4. Expression is reduced in response to radiation-induced DNA damage.2 Publications

Gene expression databases

BgeeiENSG00000149308 Expressed in 216 organ(s), highest expression level in secondary oocyte
CleanExiHS_NPAT
ExpressionAtlasiQ14207 baseline and differential
GenevisibleiQ14207 HS

Organism-specific databases

HPAiHPA045908
HPA066370

Interactioni

Subunit structurei

Interacts with the cylin/CDK complexes CCNE1/CDK2 and CCNA1/CDK2. Interacts with BZW1, CASP8AP2, CREBBP, MIZF and YY1. Interacts with the RUVBL1, RUVBL2 and TRRAP subunits of the NuA4 complex. May also interact with GAPDH, NME1, NME2 and STIP1.7 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi110924, 32 interactors
DIPiDIP-59961N
ELMiQ14207
IntActiQ14207, 10 interactors
STRINGi9606.ENSP00000278612

Structurei

3D structure databases

ProteinModelPortaliQ14207
SMRiQ14207
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 35LisHPROSITE-ProRule annotationAdd BLAST33

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 318Interaction with MIZF1 PublicationAdd BLAST318
Regioni5 – 25Required for activation of histone gene transcription and interaction with MIZF1 PublicationAdd BLAST21
Regioni121 – 145Required for activation of histone gene transcription and interaction with MIZF1 PublicationAdd BLAST25
Regioni262 – 338Mediates transcriptional activationAdd BLAST77
Regioni629 – 653Required for acceleration of G1 phaseAdd BLAST25
Regioni828 – 853Required for acceleration of G1 phaseAdd BLAST26
Regioni1039 – 1054Required for acceleration of G1 phaseAdd BLAST16
Regioni1228 – 1252Required for acceleration of G1 phaseAdd BLAST25
Regioni1325 – 1349Required for acceleration of G1 phaseAdd BLAST25

Domaini

The LisH domain is required for the activation of histone gene transcription.1 Publication

Sequence similaritiesi

Belongs to the NPAT family.Curated

Phylogenomic databases

eggNOGiENOG410IHIA Eukaryota
ENOG41112YA LUCA
GeneTreeiENSGT00390000012388
HOVERGENiHBG108198
InParanoidiQ14207
OMAiKQMVIEN
OrthoDBiEOG091G00OU
PhylomeDBiQ14207
TreeFamiTF332825

Family and domain databases

InterProiView protein in InterPro
IPR006594 LisH
IPR031442 NPAT_C
PfamiView protein in Pfam
PF15712 NPAT_C, 1 hit
SMARTiView protein in SMART
SM00667 LisH, 1 hit
PROSITEiView protein in PROSITE
PS50896 LISH, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.iShow all

Q14207-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLLPSDVARL VLGYLQQENL ISTCQTFILE SSDLKEYAEH CTDEGFIPAC
60 70 80 90 100
LLSLFGKNLT TILNEYVAMK TKETSNNVPA IMSSLWKKLD HTLSQIRSMQ
110 120 130 140 150
SSPRFAGSQR ARTRTGIAEI KRQRKLASQT APASAELLTL PYLSGQFTTP
160 170 180 190 200
PSTGTQVTRP SGQISDPSRS YFVVVNHSQS QDTVTTGEAL NVIPGAQEKK
210 220 230 240 250
AHASLMSPGR RKSESQRKST TLSGPHSTIR NFQDPNAFAV EKQMVIENAR
260 270 280 290 300
EKILSNKSLQ EKLAENINKF LTSDNNIAQV PKQTDNNPTE PETSIDEFLG
310 320 330 340 350
LPSEIHMSEE AIQDILEQTE SDPAFQALFD LFDYGKTKNN KNISQSISSQ
360 370 380 390 400
PMESNPSIVL ADETNLAVKG SFETEESDGQ SGQPAFCTSY QNDDPLNALK
410 420 430 440 450
NSNNHDVLRQ EDQENFSQIS TSIQKKAFKT AVPTEQKCDI DITFESVPNL
460 470 480 490 500
NDFNQRGNSN AECNPHCAEL YTNQMSTETE MAIGIEKNSL SSNVPSESQL
510 520 530 540 550
QPDQPDIPIT SFVSLGCEAN NENLILSGKS SQLLSQDTSL TGKPSKKSQF
560 570 580 590 600
CENSNDTVKL KINFHGSKSS DSSEVHKSKI EINVLEPVMS QLSNCQDNSC
610 620 630 640 650
LQSEILPVSV ESSHLNVSGQ VEIHLGDSLS STKQPSNDSA SVELNHTENE
660 670 680 690 700
AQASKSENSQ EPSSSVKEEN TIFLSLGGNA NCEKVALTPP EGTPVENSHS
710 720 730 740 750
LPPESVCSSV GDSHPESQNT DDKPSSNNSA EIDASNIVSL KVIISDDPFV
760 770 780 790 800
SSDTELTSAV SSINGENLPT IILSSPTKSP TKNAELVKCL SSEETVGAVV
810 820 830 840 850
YAEVGDSASM EQSLLTFKSE DSAVNNTQNE DGIAFSANVT PCVSKDGGYI
860 870 880 890 900
QLMPATSTAF GNSNNILIAT CVTDPTALGT SVSQSNVVVL PGNSAPMTAQ
910 920 930 940 950
PLPPQLQTPP RSNSVFAVNQ AVSPNFSQGS AIIIASPVQP VLQGMVGMIP
960 970 980 990 1000
VSVVGQNGNN FSTPPRQVLH MPLTAPVCNR SIPQFPVPPK SQKAQGLRNK
1010 1020 1030 1040 1050
PCIGKQVNNL VDSSGHSVGC HAQKTEVSDK SIATDLGKKS EETTVPFPEE
1060 1070 1080 1090 1100
SIVPAAKPCH RRVLCFDSTT APVANTQGPN HKMVSQNKER NAVSFPNLDS
1110 1120 1130 1140 1150
PNVSSTLKPP SNNAIKREKE KPPLPKILSK SESAISRHTT IRETQSEKKV
1160 1170 1180 1190 1200
SPTEIVLESF HKATANKENE LCSDVERQKN PENSKLSIGQ QNGGLRSEKS
1210 1220 1230 1240 1250
IASLQEMTKK QGTSSNNKNV LSVGTAVKDL KQEQTKSASS LITTEMLQDI
1260 1270 1280 1290 1300
QRHSSVSRLA DSSDLPVPRT PGSGAGEKHK EEPIDIIKAP SSRRFSEDSS
1310 1320 1330 1340 1350
TSKVMVPPVT PDLPACSPAS ETGSENSVNM AAHTLMILSR AAISRTTSAT
1360 1370 1380 1390 1400
PLKDNTQQFR ASSRSTTKKR KIEELDERER NSRPSSKNLT NSSIPMKKKK
1410 1420
IKKKKLPSSF PAGMDVDKFL LSLHYDE
Length:1,427
Mass (Da):154,290
Last modified:May 18, 2010 - v3
Checksum:iD5C5E630A56227F9
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H0YET3H0YET3_HUMAN
Protein NPAT
NPAT
299Annotation score:
E9PKJ1E9PKJ1_HUMAN
Protein NPAT
NPAT
60Annotation score:

Sequence cautioni

The sequence AAB02735 differs from that shown. Chimeric cDNA. A chimeric cDNA originating from chromosomes 11 and 4.Curated
The sequence AAH40356 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAH50561 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti22S → Y in AAB02735 (PubMed:9060412).Curated1
Sequence conflicti101S → N in BAF84376 (PubMed:14702039).Curated1
Sequence conflicti329F → L in BAF84376 (PubMed:14702039).Curated1
Sequence conflicti471Y → N in CAA65824 (PubMed:8923007).Curated1
Sequence conflicti785E → G in BAF82709 (PubMed:14702039).Curated1
Sequence conflicti1145Q → R in BAF82709 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_038696295I → L1 PublicationCorresponds to variant dbSNP:rs1131748Ensembl.1
Natural variantiVAR_038697399L → M1 PublicationCorresponds to variant dbSNP:rs1051521Ensembl.1
Natural variantiVAR_038698447V → M. Corresponds to variant dbSNP:rs35504388Ensembl.1
Natural variantiVAR_038699483I → L. Corresponds to variant dbSNP:rs968207Ensembl.1
Natural variantiVAR_038700540L → F1 PublicationCorresponds to variant dbSNP:rs4144901Ensembl.1
Natural variantiVAR_038701575V → I5 PublicationsCorresponds to variant dbSNP:rs2070661Ensembl.1
Natural variantiVAR_038702608V → A. Corresponds to variant dbSNP:rs35095430Ensembl.1
Natural variantiVAR_038703621V → I1 PublicationCorresponds to variant dbSNP:rs1051522Ensembl.1
Natural variantiVAR_038704967Q → E1 PublicationCorresponds to variant dbSNP:rs1131750Ensembl.1
Natural variantiVAR_038705973L → V1 PublicationCorresponds to variant dbSNP:rs1131751Ensembl.1
Natural variantiVAR_038706987V → A1 PublicationCorresponds to variant dbSNP:rs1051524Ensembl.1
Natural variantiVAR_038707999N → K1 PublicationCorresponds to variant dbSNP:rs34052882Ensembl.1
Natural variantiVAR_0387081191Q → R1 PublicationCorresponds to variant dbSNP:rs1051525Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83243 mRNA Translation: BAA11861.1
X97186 mRNA Translation: CAA65824.1
D89854 Genomic DNA Translation: BAA21367.1
AK290020 mRNA Translation: BAF82709.1
AK291687 mRNA Translation: BAF84376.1
BC040356 mRNA Translation: AAH40356.1 Sequence problems.
BC050561 mRNA Translation: AAH50561.1 Sequence problems.
U58852 mRNA Translation: AAB02735.1 Sequence problems.
CCDSiCCDS41710.1
RefSeqiNP_002510.2, NM_002519.2
UniGeneiHs.171061

Genome annotation databases

EnsembliENST00000278612; ENSP00000278612; ENSG00000149308
GeneIDi4863
KEGGihsa:4863
UCSCiuc001pjz.6 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiNPAT_HUMAN
AccessioniPrimary (citable) accession number: Q14207
Secondary accession number(s): A8K1V5
, A8K6M2, Q13632, Q14967, Q16580, Q86W55, Q8IWE9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: May 18, 2010
Last modified: September 12, 2018
This is version 117 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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