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Protein

Four and a half LIM domains protein 2

Gene

FHL2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth. Inhibits the transcriptional activity of FOXO1 and its apoptotic function by enhancing the interaction of FOXO1 with SIRT1 and FOXO1 deacetylation. Negatively regulates the calcineurin/NFAT signaling pathway in cardiomyocytes (PubMed:28717008).4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri7 – 31C4-typeSequence analysisAdd BLAST25

GO - Molecular functioni

  • androgen receptor binding Source: UniProtKB
  • identical protein binding Source: IntAct
  • metal ion binding Source: UniProtKB-KW
  • transcription coactivator activity Source: UniProtKB
  • transcription factor binding Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-1989781 PPARA activates gene expression
SIGNORiQ14192

Names & Taxonomyi

Protein namesi
Recommended name:
Four and a half LIM domains protein 2
Short name:
FHL-2
Alternative name(s):
LIM domain protein DRAL
Skeletal muscle LIM-protein 3
Short name:
SLIM-3
Gene namesi
Name:FHL2
Synonyms:DRAL, SLIM3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000115641.18
HGNCiHGNC:3703 FHL2
MIMi602633 gene
neXtProtiNX_Q14192

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi2274
MalaCardsiFHL2
OpenTargetsiENSG00000115641
Orphaneti154 Familial isolated dilated cardiomyopathy
PharmGKBiPA164

Polymorphism and mutation databases

BioMutaiFHL2
DMDMi116241364

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000757371 – 279Four and a half LIM domains protein 2Add BLAST279

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki78Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki167Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki220Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei238PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ14192
PaxDbiQ14192
PeptideAtlasiQ14192
PRIDEiQ14192
ProteomicsDBi59914

2D gel databases

UCD-2DPAGEiQ14192

PTM databases

iPTMnetiQ14192
PhosphoSitePlusiQ14192
SwissPalmiQ14192

Expressioni

Tissue specificityi

Expressed in skeletal muscle and heart.1 Publication

Gene expression databases

BgeeiENSG00000115641 Expressed in 215 organ(s), highest expression level in myocardium
CleanExiHS_FHL2
ExpressionAtlasiQ14192 baseline and differential
GenevisibleiQ14192 HS

Organism-specific databases

HPAiCAB008368
HPA005922
HPA006028

Interactioni

Subunit structurei

Interacts with ZNF638 and TTN/titin. Interacts with E4F1. Interacts with GRB7. Interacts with SIRT1 and FOXO1. Interacts with CEFIP (PubMed:28717008). Interacts with calcineurin (By similarity).By similarity6 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi108565, 154 interactors
CORUMiQ14192
DIPiDIP-5980N
IntActiQ14192, 171 interactors
MINTiQ14192
STRINGi9606.ENSP00000322909

Structurei

Secondary structure

1279
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ14192
SMRiQ14192
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ14192

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 92LIM zinc-binding 1PROSITE-ProRule annotationAdd BLAST53
Domaini101 – 153LIM zinc-binding 2PROSITE-ProRule annotationAdd BLAST53
Domaini162 – 212LIM zinc-binding 3PROSITE-ProRule annotationAdd BLAST51
Domaini221 – 275LIM zinc-binding 4PROSITE-ProRule annotationAdd BLAST55

Domaini

The third LIM zinc-binding mediates interaction with E4F1.

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri7 – 31C4-typeSequence analysisAdd BLAST25

Keywords - Domaini

LIM domain, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410KCU4 Eukaryota
ENOG410XRFY LUCA
GeneTreeiENSGT00760000118910
HOGENOMiHOG000231075
HOVERGENiHBG074526
InParanoidiQ14192
KOiK14380
PhylomeDBiQ14192
TreeFamiTF321684

Family and domain databases

InterProiView protein in InterPro
IPR037987 FHL2/3/5
IPR001781 Znf_LIM
PANTHERiPTHR24205 PTHR24205, 1 hit
PfamiView protein in Pfam
PF00412 LIM, 4 hits
SMARTiView protein in SMART
SM00132 LIM, 4 hits
PROSITEiView protein in PROSITE
PS00478 LIM_DOMAIN_1, 4 hits
PS50023 LIM_DOMAIN_2, 4 hits

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q14192-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MTERFDCHHC NESLFGKKYI LREESPYCVV CFETLFANTC EECGKPIGCD
60 70 80 90 100
CKDLSYKDRH WHEACFHCSQ CRNSLVDKPF AAKEDQLLCT DCYSNEYSSK
110 120 130 140 150
CQECKKTIMP GTRKMEYKGS SWHETCFICH RCQQPIGTKS FIPKDNQNFC
160 170 180 190 200
VPCYEKQHAM QCVQCKKPIT TGGVTYREQP WHKECFVCTA CRKQLSGQRF
210 220 230 240 250
TARDDFAYCL NCFCDLYAKK CAGCTNPISG LGGTKYISFE ERQWHNDCFN
260 270
CKKCSLSLVG RGFLTERDDI LCPDCGKDI
Length:279
Mass (Da):32,193
Last modified:October 17, 2006 - v3
Checksum:iE94E3A7F6601F9F3
GO
Isoform 2 (identifier: Q14192-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     53-151: DLSYKDRHWH...IPKDNQNFCV → VPARWSTRAA...SQLAMTLPTA
     152-279: Missing.

Show »
Length:151
Mass (Da):15,890
Checksum:i72F31D2DB830DB20
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
J3KNW4J3KNW4_HUMAN
Four and a half LIM domains protein...
FHL2
389Annotation score:
A0A0A0MSG2A0A0A0MSG2_HUMAN
Four and a half LIM domains protein...
FHL2
395Annotation score:
U3KQT4U3KQT4_HUMAN
Four and a half LIM domains protein...
FHL2
65Annotation score:
F8WDA8F8WDA8_HUMAN
Four and a half LIM domains protein...
FHL2
54Annotation score:
C9J3S8C9J3S8_HUMAN
Four and a half LIM domains protein...
FHL2
19Annotation score:
E9PKF4E9PKF4_HUMAN
Four and a half LIM domains protein...
FHL2
144Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_067455167K → M3 Publications1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05699953 – 151DLSYK…QNFCV → VPARWSTRAAAGMRPASSAT AASSQLEPRVSSPKTIRISV CPAMRNNMPCSAFSAKSPSP REGSLTGSSPGTRSASCAPP AGSSCLGSASQLAMTLPTA in isoform 2. 1 PublicationAdd BLAST99
Alternative sequenceiVSP_057000152 – 279Missing in isoform 2. 1 PublicationAdd BLAST128

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42176 mRNA Translation: AAA85333.1
U29332 mRNA Translation: AAC52073.1
AB038794 Genomic DNA Translation: BAA92253.1
AB158503 mRNA Translation: BAD69710.1
DQ307067 mRNA Translation: ABC25549.1
AC012360 Genomic DNA No translation available.
AC068273 Genomic DNA No translation available.
AC108058 Genomic DNA No translation available.
BC014397 mRNA Translation: AAH14397.1
U60117 mRNA Translation: AAC50794.1
CCDSiCCDS2070.1 [Q14192-1]
PIRiJC6565
RefSeqiNP_001034581.1, NM_001039492.2 [Q14192-1]
NP_001305823.1, NM_001318894.1 [Q14192-1]
NP_001305824.1, NM_001318895.1 [Q14192-1]
NP_001305825.1, NM_001318896.1 [Q14192-1]
NP_001305826.1, NM_001318897.1
NP_001441.4, NM_001450.3 [Q14192-1]
NP_963849.1, NM_201555.1 [Q14192-1]
NP_963851.2, NM_201557.3 [Q14192-1]
XP_011509100.1, XM_011510798.2 [Q14192-1]
UniGeneiHs.443687

Genome annotation databases

EnsembliENST00000322142; ENSP00000322909; ENSG00000115641 [Q14192-1]
ENST00000358129; ENSP00000350846; ENSG00000115641 [Q14192-2]
ENST00000393352; ENSP00000377020; ENSG00000115641 [Q14192-1]
ENST00000393353; ENSP00000377021; ENSG00000115641 [Q14192-1]
ENST00000408995; ENSP00000386633; ENSG00000115641 [Q14192-1]
ENST00000409807; ENSP00000386665; ENSG00000115641 [Q14192-1]
GeneIDi2274
KEGGihsa:2274
UCSCiuc061mpc.1 human [Q14192-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42176 mRNA Translation: AAA85333.1
U29332 mRNA Translation: AAC52073.1
AB038794 Genomic DNA Translation: BAA92253.1
AB158503 mRNA Translation: BAD69710.1
DQ307067 mRNA Translation: ABC25549.1
AC012360 Genomic DNA No translation available.
AC068273 Genomic DNA No translation available.
AC108058 Genomic DNA No translation available.
BC014397 mRNA Translation: AAH14397.1
U60117 mRNA Translation: AAC50794.1
CCDSiCCDS2070.1 [Q14192-1]
PIRiJC6565
RefSeqiNP_001034581.1, NM_001039492.2 [Q14192-1]
NP_001305823.1, NM_001318894.1 [Q14192-1]
NP_001305824.1, NM_001318895.1 [Q14192-1]
NP_001305825.1, NM_001318896.1 [Q14192-1]
NP_001305826.1, NM_001318897.1
NP_001441.4, NM_001450.3 [Q14192-1]
NP_963849.1, NM_201555.1 [Q14192-1]
NP_963851.2, NM_201557.3 [Q14192-1]
XP_011509100.1, XM_011510798.2 [Q14192-1]
UniGeneiHs.443687

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X4KNMR-A101-159[»]
1X4LNMR-A221-279[»]
2D8ZNMR-A162-218[»]
2MIUNMR-A1-98[»]
ProteinModelPortaliQ14192
SMRiQ14192
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108565, 154 interactors
CORUMiQ14192
DIPiDIP-5980N
IntActiQ14192, 171 interactors
MINTiQ14192
STRINGi9606.ENSP00000322909

PTM databases

iPTMnetiQ14192
PhosphoSitePlusiQ14192
SwissPalmiQ14192

Polymorphism and mutation databases

BioMutaiFHL2
DMDMi116241364

2D gel databases

UCD-2DPAGEiQ14192

Proteomic databases

EPDiQ14192
PaxDbiQ14192
PeptideAtlasiQ14192
PRIDEiQ14192
ProteomicsDBi59914

Protocols and materials databases

DNASUi2274
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000322142; ENSP00000322909; ENSG00000115641 [Q14192-1]
ENST00000358129; ENSP00000350846; ENSG00000115641 [Q14192-2]
ENST00000393352; ENSP00000377020; ENSG00000115641 [Q14192-1]
ENST00000393353; ENSP00000377021; ENSG00000115641 [Q14192-1]
ENST00000408995; ENSP00000386633; ENSG00000115641 [Q14192-1]
ENST00000409807; ENSP00000386665; ENSG00000115641 [Q14192-1]
GeneIDi2274
KEGGihsa:2274
UCSCiuc061mpc.1 human [Q14192-1]

Organism-specific databases

CTDi2274
DisGeNETi2274
EuPathDBiHostDB:ENSG00000115641.18
GeneCardsiFHL2
HGNCiHGNC:3703 FHL2
HPAiCAB008368
HPA005922
HPA006028
MalaCardsiFHL2
MIMi602633 gene
neXtProtiNX_Q14192
OpenTargetsiENSG00000115641
Orphaneti154 Familial isolated dilated cardiomyopathy
PharmGKBiPA164
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410KCU4 Eukaryota
ENOG410XRFY LUCA
GeneTreeiENSGT00760000118910
HOGENOMiHOG000231075
HOVERGENiHBG074526
InParanoidiQ14192
KOiK14380
PhylomeDBiQ14192
TreeFamiTF321684

Enzyme and pathway databases

ReactomeiR-HSA-1989781 PPARA activates gene expression
SIGNORiQ14192

Miscellaneous databases

ChiTaRSiFHL2 human
EvolutionaryTraceiQ14192
GeneWikiiFHL2
GenomeRNAii2274
PROiPR:Q14192
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000115641 Expressed in 215 organ(s), highest expression level in myocardium
CleanExiHS_FHL2
ExpressionAtlasiQ14192 baseline and differential
GenevisibleiQ14192 HS

Family and domain databases

InterProiView protein in InterPro
IPR037987 FHL2/3/5
IPR001781 Znf_LIM
PANTHERiPTHR24205 PTHR24205, 1 hit
PfamiView protein in Pfam
PF00412 LIM, 4 hits
SMARTiView protein in SMART
SM00132 LIM, 4 hits
PROSITEiView protein in PROSITE
PS00478 LIM_DOMAIN_1, 4 hits
PS50023 LIM_DOMAIN_2, 4 hits
ProtoNetiSearch...

Entry informationi

Entry nameiFHL2_HUMAN
AccessioniPrimary (citable) accession number: Q14192
Secondary accession number(s): Q13229
, Q13644, Q2I5I4, Q5TM15, Q9P294
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: October 17, 2006
Last modified: November 7, 2018
This is version 183 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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