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Protein

Double C2-like domain-containing protein beta

Gene

DOC2B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Calcium sensor which positively regulates SNARE-dependent fusion of vesicles with membranes. Binds phospholipids in a calcium-dependent manner and may act at the priming stage of fusion by modifying membrane curvature to stimulate fusion. Involved in calcium-triggered exocytosis in chromaffin cells and calcium-dependent spontaneous release of neurotransmitter in absence of action potentials in neuronal cells. Involved both in glucose-stimulated insulin secretion in pancreatic cells and insulin-dependent GLUT4 transport to the plasma membrane in adipocytes (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

LigandCalcium, Calcium/phospholipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Double C2-like domain-containing protein beta
Short name:
Doc2-beta
Gene namesi
Name:DOC2BImported
Synonyms:DOC2BLImported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000272636.3
HGNCiHGNC:2986 DOC2B
MIMi604568 gene
neXtProtiNX_Q14184

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi8447
OpenTargetsiENSG00000272636
PharmGKBiPA27452

Polymorphism and mutation databases

DMDMi51701386

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000799681 – 412Double C2-like domain-containing protein betaAdd BLAST412

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei411PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ14184
PeptideAtlasiQ14184
PRIDEiQ14184
ProteomicsDBi59902

PTM databases

iPTMnetiQ14184
PhosphoSitePlusiQ14184

Expressioni

Tissue specificityi

Widely expressed with highest levels in brain and kidney. Expressed in pancreatic islet cells (at protein level).2 Publications

Gene expression databases

BgeeiENSG00000272636 Expressed in 152 organ(s), highest expression level in cerebellar vermis
CleanExiHS_DOC2B

Organism-specific databases

HPAiHPA043168

Interactioni

Subunit structurei

Interacts with the SNARE (soluble N-ethylmaleimide-sensitive factor attached protein receptor) complex composed of SNAP25, STX1A and VAMP2; the interaction is calcium-dependent and competitive with SYT1. Interacts with STX4; the interaction is calcium-dependent, increased by insulin and glucose, and mediates vesicle fusion with plasma membrane in pancreatic cells and adipocytes. Interacts with STXBP3; the interaction is direct, occurs at the cell membrane and regulates glucose-stimulated insulin secretion. May interact with UNC13A; the interaction mediates targeting to the plasma membrane (By similarity). Interacts with cytoplasmic dynein light chain DYNLT1.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi114025, 2 interactors
IntActiQ14184, 1 interactor
MINTiQ14184
STRINGi9606.ENSP00000343665

Structurei

3D structure databases

ProteinModelPortaliQ14184
SMRiQ14184
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini128 – 232C2 1PROSITE-ProRule annotationAdd BLAST105
Domaini268 – 371C2 2PROSITE-ProRule annotationAdd BLAST104

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 90Mediates interaction with DYNLT11 PublicationAdd BLAST90
Regioni1 – 36Negatively regulates targeting to plasma membraneBy similarityAdd BLAST36
Regioni257 – 375Mediates interaction with STXBP3By similarityAdd BLAST119

Domaini

C2 domain 1 is involved in binding calcium and phospholipids. C2 domain 2 may also play a role in the calcium-dependent targeting to membranes (By similarity).By similarity

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1013 Eukaryota
ENOG410XQXA LUCA
GeneTreeiENSGT00810000125376
HOGENOMiHOG000232127
HOVERGENiHBG051388
InParanoidiQ14184
KOiK19917
OMAiKANHTKT
OrthoDBiEOG091G0FPP
PhylomeDBiQ14184
TreeFamiTF351844

Family and domain databases

Gene3Di2.60.40.150, 2 hits
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR014638 Doc2
IPR030534 Doc2b
IPR001565 Synaptotagmin
PANTHERiPTHR10024:SF204 PTHR10024:SF204, 2 hits
PfamiView protein in Pfam
PF00168 C2, 2 hits
PIRSFiPIRSF036931 Doc2, 1 hit
PRINTSiPR00360 C2DOMAIN
PR00399 SYNAPTOTAGMN
SMARTiView protein in SMART
SM00239 C2, 2 hits
PROSITEiView protein in PROSITE
PS50004 C2, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q14184-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTLRRRGEKA TISIQEHMAI DVCPGPIRPI KQISDYFPRF PRGLPPDAGP
60 70 80 90 100
RAAAPPDAPA RPAVAGAGRR SPSDGAREDD EDVDQLFGAY GSSPGPSPGP
110 120 130 140 150
SPARPPAKPP EDEPDADGYE SDDCTALGTL DFSLLYDQEN NALHCTITKA
160 170 180 190 200
KGLKPMDHNG LADPYVKLHL LPGASKANKL RTKTLRNTLN PTWNETLTYY
210 220 230 240 250
GITDEDMIRK TLRISVCDED KFRHNEFIGE TRVPLKKLKP NHTKTFSICL
260 270 280 290 300
EKQLPVDKTE DKSLEERGRI LISLKYSSQK QGLLVGIVRC AHLAAMDANG
310 320 330 340 350
YSDPYVKTYL RPDVDKKSKH KTAVKKKTLN PEFNEEFCYE IKHGDLAKKS
360 370 380 390 400
LEVTVWDYDI GKSNDFIGGV VLGIHAKGER LKHWFDCLKN KDKRIERWHT
410
LTSELPGAVL SD
Length:412
Mass (Da):45,922
Last modified:March 28, 2018 - v2
Checksum:i8D8D2BB3FE77F3E5
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0G2JKH4A0A0G2JKH4_HUMAN
Double C2-like domain-containing pr...
DOC2B
412Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti247S → N in BAA11107 (PubMed:8554557).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_065743209R → L in a patient with amyotrophic lateral sclerosis. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D70830 mRNA Translation: BAA11107.1
AC108004 Genomic DNA No translation available.
AC240565 Genomic DNA No translation available.
KF511233 Genomic DNA No translation available.
CH471108 Genomic DNA Translation: EAW90677.1
CCDSiCCDS73934.1
RefSeqiNP_003576.2, NM_003585.4
UniGeneiHs.551705

Genome annotation databases

EnsembliENST00000613549; ENSP00000482950; ENSG00000272636
GeneIDi8447
KEGGihsa:8447

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D70830 mRNA Translation: BAA11107.1
AC108004 Genomic DNA No translation available.
AC240565 Genomic DNA No translation available.
KF511233 Genomic DNA No translation available.
CH471108 Genomic DNA Translation: EAW90677.1
CCDSiCCDS73934.1
RefSeqiNP_003576.2, NM_003585.4
UniGeneiHs.551705

3D structure databases

ProteinModelPortaliQ14184
SMRiQ14184
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114025, 2 interactors
IntActiQ14184, 1 interactor
MINTiQ14184
STRINGi9606.ENSP00000343665

PTM databases

iPTMnetiQ14184
PhosphoSitePlusiQ14184

Polymorphism and mutation databases

DMDMi51701386

Proteomic databases

PaxDbiQ14184
PeptideAtlasiQ14184
PRIDEiQ14184
ProteomicsDBi59902

Protocols and materials databases

DNASUi8447
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000613549; ENSP00000482950; ENSG00000272636
GeneIDi8447
KEGGihsa:8447

Organism-specific databases

CTDi8447
DisGeNETi8447
EuPathDBiHostDB:ENSG00000272636.3
GeneCardsiDOC2B
HGNCiHGNC:2986 DOC2B
HPAiHPA043168
MIMi604568 gene
neXtProtiNX_Q14184
OpenTargetsiENSG00000272636
PharmGKBiPA27452
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1013 Eukaryota
ENOG410XQXA LUCA
GeneTreeiENSGT00810000125376
HOGENOMiHOG000232127
HOVERGENiHBG051388
InParanoidiQ14184
KOiK19917
OMAiKANHTKT
OrthoDBiEOG091G0FPP
PhylomeDBiQ14184
TreeFamiTF351844

Miscellaneous databases

GeneWikiiDOC2B
GenomeRNAii8447
PROiPR:Q14184
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000272636 Expressed in 152 organ(s), highest expression level in cerebellar vermis
CleanExiHS_DOC2B

Family and domain databases

Gene3Di2.60.40.150, 2 hits
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR014638 Doc2
IPR030534 Doc2b
IPR001565 Synaptotagmin
PANTHERiPTHR10024:SF204 PTHR10024:SF204, 2 hits
PfamiView protein in Pfam
PF00168 C2, 2 hits
PIRSFiPIRSF036931 Doc2, 1 hit
PRINTSiPR00360 C2DOMAIN
PR00399 SYNAPTOTAGMN
SMARTiView protein in SMART
SM00239 C2, 2 hits
PROSITEiView protein in PROSITE
PS50004 C2, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiDOC2B_HUMAN
AccessioniPrimary (citable) accession number: Q14184
Secondary accession number(s): A0A087WZX8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: March 28, 2018
Last modified: November 7, 2018
This is version 147 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  2. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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