UniProtKB - Q14152 (EIF3A_HUMAN)
Eukaryotic translation initiation factor 3 subunit A
EIF3A
Functioni
GO - Molecular functioni
- mRNA binding Source: GO_Central
- receptor tyrosine kinase binding Source: Ensembl
- RNA binding Source: UniProtKB
- structural molecule activity Source: UniProtKB
- translation initiation factor activity Source: UniProtKB-UniRule
GO - Biological processi
- formation of cytoplasmic translation initiation complex Source: UniProtKB
- IRES-dependent viral translational initiation Source: UniProtKB
- negative regulation of ERK1 and ERK2 cascade Source: Ensembl
- translational initiation Source: Reactome
- translation reinitiation Source: GO_Central
- viral translational termination-reinitiation Source: UniProtKB
Keywordsi
Molecular function | Initiation factor, RNA-binding |
Biological process | Host-virus interaction, Protein biosynthesis |
Enzyme and pathway databases
PathwayCommonsi | Q14152 |
Reactomei | R-HSA-156827, L13a-mediated translational silencing of Ceruloplasmin expression R-HSA-72649, Translation initiation complex formation R-HSA-72689, Formation of a pool of free 40S subunits R-HSA-72695, Formation of the ternary complex, and subsequently, the 43S complex R-HSA-72702, Ribosomal scanning and start codon recognition R-HSA-72706, GTP hydrolysis and joining of the 60S ribosomal subunit |
SIGNORi | Q14152 |
Protein family/group databases
MoonProti | Q14152 |
Names & Taxonomyi
Protein namesi | Recommended name: Eukaryotic translation initiation factor 3 subunit AUniRule annotationShort name: eIF3aUniRule annotation Alternative name(s): Eukaryotic translation initiation factor 3 subunit 10UniRule annotation eIF-3-thetaUniRule annotation eIF3 p167 eIF3 p180 eIF3 p185 |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000107581.12 |
HGNCi | HGNC:3271, EIF3A |
MIMi | 602039, gene |
neXtProti | NX_Q14152 |
Subcellular locationi
Other locations
- Cytoplasm UniRule annotation1 Publication
Cytoskeleton
- microtubule Source: Ensembl
Cytosol
- cytosol Source: HPA
Nucleus
- nucleolus Source: HPA
- nucleoplasm Source: HPA
Other locations
- cytoplasm Source: UniProtKB
- eukaryotic 43S preinitiation complex Source: UniProtKB-UniRule
- eukaryotic 48S preinitiation complex Source: UniProtKB-UniRule
- eukaryotic translation initiation factor 3 complex Source: UniProtKB
- eukaryotic translation initiation factor 3 complex, eIF3e Source: GO_Central
- eukaryotic translation initiation factor 3 complex, eIF3m Source: GO_Central
- membrane Source: UniProtKB
- multi-eIF complex Source: GO_Central
- postsynaptic density Source: Ensembl
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Organism-specific databases
DisGeNETi | 8661 |
OpenTargetsi | ENSG00000107581 |
PharmGKBi | PA27699 |
Miscellaneous databases
Pharosi | Q14152, Tbio |
Polymorphism and mutation databases
BioMutai | EIF3A |
DMDMi | 6685537 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedUniRule annotation2 Publications | |||
ChainiPRO_0000123537 | 2 – 1382 | Eukaryotic translation initiation factor 3 subunit AAdd BLAST | 1381 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 68 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 492 | PhosphoserineCombined sources | 1 | |
Modified residuei | 584 | PhosphoserineCombined sources | 1 | |
Modified residuei | 881 | PhosphoserineUniRule annotationCombined sources1 Publication | 1 | |
Modified residuei | 882 | PhosphoserineCombined sources | 1 | |
Modified residuei | 895 | PhosphoserineCombined sources | 1 | |
Modified residuei | 949 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1028 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1188 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1198 | PhosphoserineUniRule annotationCombined sources1 Publication | 1 | |
Modified residuei | 1262 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1336 | PhosphoserineUniRule annotationCombined sources1 Publication | 1 | |
Modified residuei | 1364 | PhosphoserineUniRule annotation1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q14152 |
jPOSTi | Q14152 |
MassIVEi | Q14152 |
MaxQBi | Q14152 |
PaxDbi | Q14152 |
PeptideAtlasi | Q14152 |
PRIDEi | Q14152 |
ProteomicsDBi | 26155 59854 [Q14152-1] |
2D gel databases
UCD-2DPAGEi | Q14152 |
PTM databases
iPTMneti | Q14152 |
MetOSitei | Q14152 |
PhosphoSitePlusi | Q14152 |
SwissPalmi | Q14152 |
Expressioni
Gene expression databases
Bgeei | ENSG00000107581, Expressed in substantia nigra and 250 other tissues |
Genevisiblei | Q14152, HS |
Organism-specific databases
HPAi | ENSG00000107581, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with EIF4G1 (By similarity).
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3L and EIF3K. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3.
Also interacts with KRT7 and PIWIL2.
By similarity8 PublicationsBinary interactionsi
Hide detailsQ14152
GO - Molecular functioni
- receptor tyrosine kinase binding Source: Ensembl
Protein-protein interaction databases
BioGRIDi | 114210, 174 interactors |
CORUMi | Q14152 |
DIPi | DIP-31114N |
IntActi | Q14152, 94 interactors |
MINTi | Q14152 |
STRINGi | 9606.ENSP00000358140 |
Miscellaneous databases
RNActi | Q14152, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q14152 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 315 – 498 | PCIPROSITE-ProRule annotationAdd BLAST | 184 | |
Repeati | 925 – 934 | 1 | 10 | |
Repeati | 935 – 942 | 2; truncated | 8 | |
Repeati | 943 – 952 | 3 | 10 | |
Repeati | 953 – 962 | 4 | 10 | |
Repeati | 963 – 972 | 5 | 10 | |
Repeati | 973 – 982 | 6 | 10 | |
Repeati | 983 – 992 | 7 | 10 | |
Repeati | 993 – 1002 | 8 | 10 | |
Repeati | 1003 – 1012 | 9 | 10 | |
Repeati | 1013 – 1022 | 10 | 10 | |
Repeati | 1023 – 1032 | 11 | 10 | |
Repeati | 1033 – 1042 | 12 | 10 | |
Repeati | 1043 – 1052 | 13 | 10 | |
Repeati | 1054 – 1063 | 14 | 10 | |
Repeati | 1064 – 1073 | 15 | 10 | |
Repeati | 1074 – 1083 | 16 | 10 | |
Repeati | 1084 – 1093 | 17 | 10 | |
Repeati | 1094 – 1103 | 18 | 10 | |
Repeati | 1104 – 1113 | 19 | 10 | |
Repeati | 1114 – 1123 | 20 | 10 | |
Repeati | 1124 – 1133 | 21 | 10 | |
Repeati | 1134 – 1143 | 22 | 10 | |
Repeati | 1144 – 1152 | 23; truncated | 9 | |
Repeati | 1153 – 1162 | 24 | 10 | |
Repeati | 1163 – 1172 | 25; approximate | 10 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 664 – 835 | Interaction with EIF3BAdd BLAST | 172 | |
Regioni | 925 – 1172 | 25 X 10 AA approximate tandem repeats of [DE]-[DE]-[DE]-R-[SEVGFPILV]-[HPSN]-[RSW]-[RL]-[DRGTIHN]-[EPMANLGDT]Add BLAST | 248 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 82 – 120 | UniRule annotationAdd BLAST | 39 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 576 – 875 | Glu-richAdd BLAST | 300 | |
Compositional biasi | 925 – 1294 | Asp-richAdd BLAST | 370 |
Sequence similaritiesi
Keywords - Domaini
Coiled coil, RepeatPhylogenomic databases
eggNOGi | KOG2072, Eukaryota |
GeneTreei | ENSGT00730000111063 |
HOGENOMi | CLU_002096_1_1_1 |
InParanoidi | Q14152 |
OMAi | LEVMYQT |
OrthoDBi | 967904at2759 |
PhylomeDBi | Q14152 |
TreeFami | TF101522 |
Family and domain databases
HAMAPi | MF_03000, eIF3a, 1 hit |
InterProi | View protein in InterPro IPR027512, EIF3A IPR000717, PCI_dom |
PANTHERi | PTHR14005, PTHR14005, 1 hit |
Pfami | View protein in Pfam PF01399, PCI, 1 hit |
SMARTi | View protein in SMART SM00088, PINT, 1 hit |
PROSITEi | View protein in PROSITE PS50250, PCI, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MPAYFQRPEN ALKRANEFLE VGKKQPALDV LYDVMKSKKH RTWQKIHEPI
60 70 80 90 100
MLKYLELCVD LRKSHLAKEG LYQYKNICQQ VNIKSLEDVV RAYLKMAEEK
110 120 130 140 150
TEAAKEESQQ MVLDIEDLDN IQTPESVLLS AVSGEDTQDR TDRLLLTPWV
160 170 180 190 200
KFLWESYRQC LDLLRNNSRV ERLYHDIAQQ AFKFCLQYTR KAEFRKLCDN
210 220 230 240 250
LRMHLSQIQR HHNQSTAINL NNPESQSMHL ETRLVQLDSA ISMELWQEAF
260 270 280 290 300
KAVEDIHGLF SLSKKPPKPQ LMANYYNKVS TVFWKSGNAL FHASTLHRLY
310 320 330 340 350
HLSREMRKNL TQDEMQRMST RVLLATLSIP ITPERTDIAR LLDMDGIIVE
360 370 380 390 400
KQRRLATLLG LQAPPTRIGL INDMVRFNVL QYVVPEVKDL YNWLEVEFNP
410 420 430 440 450
LKLCERVTKV LNWVREQPEK EPELQQYVPQ LQNNTILRLL QQVSQIYQSI
460 470 480 490 500
EFSRLTSLVP FVDAFQLERA IVDAARHCDL QVRIDHTSRT LSFGSDLNYA
510 520 530 540 550
TREDAPIGPH LQSMPSEQIR NQLTAMSSVL AKALEVIKPA HILQEKEEQH
560 570 580 590 600
QLAVTAYLKN SRKEHQRILA RRQTIEERKE RLESLNIQRE KEELEQREAE
610 620 630 640 650
LQKVRKAEEE RLRQEAKERE KERILQEHEQ IKKKTVRERL EQIKKTELGA
660 670 680 690 700
KAFKDIDIED LEELDPDFIM AKQVEQLEKE KKELQERLKN QEKKIDYFER
710 720 730 740 750
AKRLEEIPLI KSAYEEQRIK DMDLWEQQEE ERITTMQLER EKALEHKNRM
760 770 780 790 800
SRMLEDRDLF VMRLKAARQS VYEEKLKQFE ERLAEERHNR LEERKRQRKE
810 820 830 840 850
ERRITYYREK EEEEQRRAEE QMLKEREERE RAERAKREEE LREYQERVKK
860 870 880 890 900
LEEVERKKRQ RELEIEERER RREEERRLGD SSLSRKDSRW GDRDSEGTWR
910 920 930 940 950
KGPEADSEWR RGPPEKEWRR GEGRDEDRSH RRDEERPRRL GDDEDREPSL
960 970 980 990 1000
RPDDDRVPRR GMDDDRGPRR GPEEDRFSRR GADDDRPSWR NTDDDRPPRR
1010 1020 1030 1040 1050
IADEDRGNWR HADDDRPPRR GLDEDRGSWR TADEDRGPRR GMDDDRGPRR
1060 1070 1080 1090 1100
GGADDERSSW RNADDDRGPR RGLDDDRGPR RGMDDDRGPR RGMDDDRGPR
1110 1120 1130 1140 1150
RGMDDDRGPR RGLDDDRGPW RNADDDRIPR RGAEDDRGPW RNMDDDRLSR
1160 1170 1180 1190 1200
RADDDRFPRR GDDSRPGPWR PLVKPGGWRE KEKAREESWG PPRESRPSEE
1210 1220 1230 1240 1250
REWDREKERD RDNQDREEND KDPERERDRE RDVDREDRFR RPRDEGGWRR
1260 1270 1280 1290 1300
GPAEESSSWR DSSRRDDRDR DDRRRERDDR RDLRERRDLR DDRDRRGPPL
1310 1320 1330 1340 1350
RSEREEVSSW RRADDRKDDR VEERDPPRRV PPPALSRDRE RDRDREREGE
1360 1370 1380
KEKASWRAEK DRESLRRTKN ETDEDGWTTV RR
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 520 | R → G in BAG63833 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 983 | D → G in BAG63833 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 1272 | D → G in BAG63833 (PubMed:14702039).Curated | 1 |
Mass spectrometryi
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_024438 | 386 | E → K1 PublicationCorresponds to variant dbSNP:rs967185Ensembl. | 1 | |
Natural variantiVAR_048921 | 694 | K → N. Corresponds to variant dbSNP:rs431898Ensembl. | 1 | |
Natural variantiVAR_048922 | 993 | D → E. Corresponds to variant dbSNP:rs532138EnsemblClinVar. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_055471 | 1 – 34 | Missing in isoform 2. 1 PublicationAdd BLAST | 34 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U58046 mRNA Translation: AAB41584.1 U58047 Genomic DNA Translation: AAB41586.1 U78311 mRNA Translation: AAB80695.1 D50929 mRNA Translation: BAA09488.2 Different initiation. AK302575 mRNA Translation: BAG63833.1 AL355598 Genomic DNA No translation available. CH471066 Genomic DNA Translation: EAW49408.1 |
CCDSi | CCDS7608.1 [Q14152-1] |
RefSeqi | NP_003741.1, NM_003750.2 [Q14152-1] |
Genome annotation databases
Ensembli | ENST00000369144; ENSP00000358140; ENSG00000107581 [Q14152-1] ENST00000541549; ENSP00000438178; ENSG00000107581 [Q14152-1] |
GeneIDi | 8661 |
KEGGi | hsa:8661 |
UCSCi | uc001ldu.4, human [Q14152-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U58046 mRNA Translation: AAB41584.1 U58047 Genomic DNA Translation: AAB41586.1 U78311 mRNA Translation: AAB80695.1 D50929 mRNA Translation: BAA09488.2 Different initiation. AK302575 mRNA Translation: BAG63833.1 AL355598 Genomic DNA No translation available. CH471066 Genomic DNA Translation: EAW49408.1 |
CCDSi | CCDS7608.1 [Q14152-1] |
RefSeqi | NP_003741.1, NM_003750.2 [Q14152-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3J8B | electron microscopy | - | A | 1-494 | [»] | |
3J8C | electron microscopy | - | A | 1-494 | [»] | |
6ZON | electron microscopy | 3.00 | A | 1-601 | [»] | |
6ZP4 | electron microscopy | 2.90 | A | 1-1382 | [»] | |
SMRi | Q14152 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 114210, 174 interactors |
CORUMi | Q14152 |
DIPi | DIP-31114N |
IntActi | Q14152, 94 interactors |
MINTi | Q14152 |
STRINGi | 9606.ENSP00000358140 |
Protein family/group databases
MoonProti | Q14152 |
PTM databases
iPTMneti | Q14152 |
MetOSitei | Q14152 |
PhosphoSitePlusi | Q14152 |
SwissPalmi | Q14152 |
Polymorphism and mutation databases
BioMutai | EIF3A |
DMDMi | 6685537 |
2D gel databases
UCD-2DPAGEi | Q14152 |
Proteomic databases
EPDi | Q14152 |
jPOSTi | Q14152 |
MassIVEi | Q14152 |
MaxQBi | Q14152 |
PaxDbi | Q14152 |
PeptideAtlasi | Q14152 |
PRIDEi | Q14152 |
ProteomicsDBi | 26155 59854 [Q14152-1] |
Protocols and materials databases
Antibodypediai | 32084, 270 antibodies |
Genome annotation databases
Ensembli | ENST00000369144; ENSP00000358140; ENSG00000107581 [Q14152-1] ENST00000541549; ENSP00000438178; ENSG00000107581 [Q14152-1] |
GeneIDi | 8661 |
KEGGi | hsa:8661 |
UCSCi | uc001ldu.4, human [Q14152-1] |
Organism-specific databases
CTDi | 8661 |
DisGeNETi | 8661 |
EuPathDBi | HostDB:ENSG00000107581.12 |
GeneCardsi | EIF3A |
HGNCi | HGNC:3271, EIF3A |
HPAi | ENSG00000107581, Low tissue specificity |
MIMi | 602039, gene |
neXtProti | NX_Q14152 |
OpenTargetsi | ENSG00000107581 |
PharmGKBi | PA27699 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2072, Eukaryota |
GeneTreei | ENSGT00730000111063 |
HOGENOMi | CLU_002096_1_1_1 |
InParanoidi | Q14152 |
OMAi | LEVMYQT |
OrthoDBi | 967904at2759 |
PhylomeDBi | Q14152 |
TreeFami | TF101522 |
Enzyme and pathway databases
PathwayCommonsi | Q14152 |
Reactomei | R-HSA-156827, L13a-mediated translational silencing of Ceruloplasmin expression R-HSA-72649, Translation initiation complex formation R-HSA-72689, Formation of a pool of free 40S subunits R-HSA-72695, Formation of the ternary complex, and subsequently, the 43S complex R-HSA-72702, Ribosomal scanning and start codon recognition R-HSA-72706, GTP hydrolysis and joining of the 60S ribosomal subunit |
SIGNORi | Q14152 |
Miscellaneous databases
BioGRID-ORCSi | 8661, 760 hits in 848 CRISPR screens |
ChiTaRSi | EIF3A, human |
GeneWikii | EIF3A |
GenomeRNAii | 8661 |
Pharosi | Q14152, Tbio |
PROi | PR:Q14152 |
RNActi | Q14152, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000107581, Expressed in substantia nigra and 250 other tissues |
Genevisiblei | Q14152, HS |
Family and domain databases
HAMAPi | MF_03000, eIF3a, 1 hit |
InterProi | View protein in InterPro IPR027512, EIF3A IPR000717, PCI_dom |
PANTHERi | PTHR14005, PTHR14005, 1 hit |
Pfami | View protein in Pfam PF01399, PCI, 1 hit |
SMARTi | View protein in SMART SM00088, PINT, 1 hit |
PROSITEi | View protein in PROSITE PS50250, PCI, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | EIF3A_HUMAN | |
Accessioni | Q14152Primary (citable) accession number: Q14152 Secondary accession number(s): B1AMV5 Q15778 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | November 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 202 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 10
Human chromosome 10: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations - Translation initiation factors
List of translation initiation factor entries