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Protein

Heterogeneous nuclear ribonucleoprotein D0

Gene

HNRNPD

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs. Also binds to double- and single-stranded DNA sequences in a specific manner and functions a transcription factor. Each of the RNA-binding domains specifically can bind solely to a single-stranded non-monotonous 5'-UUAG-3' sequence and also weaker to the single-stranded 5'-TTAGGG-3' telomeric DNA repeat. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. Binding of RRM1 to DNA inhibits the formation of DNA quadruplex structure which may play a role in telomere elongation. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. May play a role in the regulation of the rhythmic expression of circadian clock core genes. Directly binds to the 3'UTR of CRY1 mRNA and induces CRY1 rhythmic translation. May also be involved in the regulation of PER2 translation.3 Publications

GO - Molecular functioni

  • AT DNA binding Source: Ensembl
  • chromatin binding Source: Ensembl
  • histone deacetylase binding Source: Ensembl
  • mRNA 3'-UTR AU-rich region binding Source: Ensembl
  • RNA binding Source: UniProtKB
  • telomeric DNA binding Source: UniProtKB
  • transcription factor binding Source: Ensembl

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Ribonucleoprotein, RNA-binding
Biological processBiological rhythms, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-450408 AUF1 (hnRNP D0) binds and destabilizes mRNA
R-HSA-72163 mRNA Splicing - Major Pathway
R-HSA-72203 Processing of Capped Intron-Containing Pre-mRNA
SIGNORiQ14103

Protein family/group databases

MoonDBiQ14103 Predicted

Names & Taxonomyi

Protein namesi
Recommended name:
Heterogeneous nuclear ribonucleoprotein D0
Short name:
hnRNP D0
Alternative name(s):
AU-rich element RNA-binding protein 1
Gene namesi
Name:HNRNPD
Synonyms:AUF1, HNRPD
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

EuPathDBiHostDB:ENSG00000138668.18
HGNCiHGNC:5036 HNRNPD
MIMi601324 gene
neXtProtiNX_Q14103

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi3184
OpenTargetsiENSG00000138668
PharmGKBiPA29361

Polymorphism and mutation databases

BioMutaiHNRNPD
DMDMi13124489

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000818492 – 355Heterogeneous nuclear ribonucleoprotein D0Add BLAST354

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei71PhosphoserineCombined sources1
Cross-linki72Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei80PhosphoserineCombined sources1
Modified residuei82PhosphoserineCombined sources1
Modified residuei83PhosphoserineCombined sources1
Modified residuei91PhosphothreonineCombined sources1
Modified residuei119N6-methyllysine1 Publication1
Modified residuei127PhosphothreonineCombined sources1
Cross-linki129Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei165N6-acetyllysineCombined sources1
Modified residuei190PhosphoserineCombined sources1
Modified residuei193PhosphothreonineCombined sources1
Cross-linki197Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei243N6-acetyllysineBy similarity1
Modified residuei251N6-acetyllysineCombined sources1
Modified residuei271PhosphoserineCombined sources1
Modified residuei272Omega-N-methylarginineCombined sources1
Modified residuei278Omega-N-methylarginineCombined sources1
Modified residuei280Omega-N-methylarginineCombined sources1
Modified residuei282Omega-N-methylarginineBy similarity1
Modified residuei345Asymmetric dimethylarginine; alternateBy similarity1
Modified residuei345Dimethylated arginine; alternateCombined sources1
Modified residuei345Omega-N-methylarginine; alternateCombined sources1
Isoform 4 (identifier: Q14103-4)
Modified residuei263Omega-N-methylarginineCombined sources1
Modified residuei273N6-acetyllysineCombined sources1
Isoform 3 (identifier: Q14103-3)
Modified residuei282Omega-N-methylarginineCombined sources1
Modified residuei292N6-acetyllysineCombined sources1

Post-translational modificationi

Arg-345 is dimethylated, probably to asymmetric dimethylarginine.
Methylated by PRMT1, in an insulin-dependent manner. The PRMT1-mediated methylation regulates tyrosine phosphorylation (By similarity).By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ14103
MaxQBiQ14103
PaxDbiQ14103
PeptideAtlasiQ14103
PRIDEiQ14103
ProteomicsDBi59811
59812 [Q14103-2]
59813 [Q14103-3]
59814 [Q14103-4]
TopDownProteomicsiQ14103-1 [Q14103-1]
Q14103-2 [Q14103-2]
Q14103-3 [Q14103-3]

2D gel databases

SWISS-2DPAGEiQ14103

PTM databases

iPTMnetiQ14103
PhosphoSitePlusiQ14103
SwissPalmiQ14103

Miscellaneous databases

PMAP-CutDBiQ14103

Expressioni

Gene expression databases

BgeeiENSG00000138668
ExpressionAtlasiQ14103 baseline and differential
GenevisibleiQ14103 HS

Organism-specific databases

HPAiHPA004911

Interactioni

Subunit structurei

Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Part of a complex associated with the FOS mCRD domain and consisting of PABPC1, PAIP1, CSDE1/UNR and SYNCRIP. Interacts with IGF2BP2. Interacts with GTPBP1. Interacts with EIF4G1; the interaction requires RNA. Interacts with EIF3B and RPS3.5 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi109425, 279 interactors
ComplexPortaliCPX-1076 mCRD-poly(A)-bridging complex
CORUMiQ14103
DIPiDIP-31163N
IntActiQ14103, 148 interactors
MINTiQ14103
STRINGi9606.ENSP00000313199

Structurei

Secondary structure

1355
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi97 – 102Combined sources6
Helixi110 – 117Combined sources8
Helixi118 – 120Combined sources3
Beta strandi123 – 130Combined sources8
Turni132 – 134Combined sources3
Beta strandi137 – 147Combined sources11
Helixi149 – 155Combined sources7
Beta strandi160 – 162Combined sources3
Beta strandi165 – 171Combined sources7
Helixi172 – 174Combined sources3
Beta strandi184 – 187Combined sources4
Helixi195 – 205Combined sources11
Beta strandi209 – 211Combined sources3
Turni217 – 219Combined sources3
Beta strandi220 – 222Combined sources3
Beta strandi226 – 229Combined sources4
Beta strandi231 – 233Combined sources3
Helixi234 – 240Combined sources7
Beta strandi244 – 247Combined sources4
Beta strandi250 – 253Combined sources4
Beta strandi254 – 256Combined sources3
Beta strandi349 – 351Combined sources3

3D structure databases

ProteinModelPortaliQ14103
SMRiQ14103
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ14103

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini97 – 179RRM 1PROSITE-ProRule annotationAdd BLAST83
Domaini182 – 261RRM 2PROSITE-ProRule annotationAdd BLAST80

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi11 – 45Ala-richAdd BLAST35
Compositional biasi270 – 347Gly-richAdd BLAST78
Compositional biasi294 – 332Tyr-richAdd BLAST39

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0118 Eukaryota
COG0724 LUCA
GeneTreeiENSGT00900000140835
HOVERGENiHBG002295
InParanoidiQ14103
KOiK13044
OMAiKKYHNIG
OrthoDBiEOG091G1CPI
PhylomeDBiQ14103
TreeFamiTF314808

Family and domain databases

Gene3Di3.30.70.330, 2 hits
InterProiView protein in InterPro
IPR012956 CARG-binding_factor_N
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PfamiView protein in Pfam
PF08143 CBFNT, 1 hit
PF00076 RRM_1, 2 hits
SMARTiView protein in SMART
SM00360 RRM, 2 hits
SUPFAMiSSF54928 SSF54928, 2 hits
PROSITEiView protein in PROSITE
PS50102 RRM, 2 hits

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q14103-1) [UniParc]FASTAAdd to basket
Also known as: p45, Dx9

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSEEQFGGDG AAAAATAAVG GSAGEQEGAM VAATQGAAAA AGSGAGTGGG
60 70 80 90 100
TASGGTEGGS AESEGAKIDA SKNEEDEGHS NSSPRHSEAA TAQREEWKMF
110 120 130 140 150
IGGLSWDTTK KDLKDYFSKF GEVVDCTLKL DPITGRSRGF GFVLFKESES
160 170 180 190 200
VDKVMDQKEH KLNGKVIDPK RAKAMKTKEP VKKIFVGGLS PDTPEEKIRE
210 220 230 240 250
YFGGFGEVES IELPMDNKTN KRRGFCFITF KEEEPVKKIM EKKYHNVGLS
260 270 280 290 300
KCEIKVAMSK EQYQQQQQWG SRGGFAGRAR GRGGGPSQNW NQGYSNYWNQ
310 320 330 340 350
GYGNYGYNSQ GYGGYGGYDY TGYNNYYGYG DYSNQQSGYG KVSRRGGHQN

SYKPY
Length:355
Mass (Da):38,434
Last modified:November 1, 1996 - v1
Checksum:iD0B6EA177BEF789E
GO
Isoform 2 (identifier: Q14103-2) [UniParc]FASTAAdd to basket
Also known as: p42, Dx4

The sequence of this isoform differs from the canonical sequence as follows:
     79-97: Missing.

Show »
Length:336
Mass (Da):36,272
Checksum:iFEE18D61B7714B51
GO
Isoform 3 (identifier: Q14103-3) [UniParc]FASTAAdd to basket
Also known as: p40, Dx7

The sequence of this isoform differs from the canonical sequence as follows:
     285-334: GPSQNWNQGYSNYWNQGYGNYGYNSQGYGGYGGYDYTGYNNYYGYGDYSN → D

Show »
Length:306
Mass (Da):32,835
Checksum:iABCDD6ACF812F647
GO
Isoform 4 (identifier: Q14103-4) [UniParc]FASTAAdd to basket
Also known as: p37

The sequence of this isoform differs from the canonical sequence as follows:
     79-97: Missing.
     285-334: GPSQNWNQGYSNYWNQGYGNYGYNSQGYGGYGGYDYTGYNNYYGYGDYSN → D

Show »
Length:287
Mass (Da):30,672
Checksum:i98DF6E78EAF3BBC1
GO

Sequence cautioni

The sequence AAA35781 differs from that shown. Contaminating sequence. Sequence of unknown origin in the N-terminal part.Curated
The sequence AAA35781 differs from that shown. Reason: Frameshift at positions 45, 59 and 355.Curated
The sequence CAA27544 differs from that shown. Several sequence conflicts.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti150S → R AA sequence (PubMed:8321232).Curated1
Sequence conflicti225F → L in AAA35781 (PubMed:1433497).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00583479 – 97Missing in isoform 2 and isoform 4. 2 PublicationsAdd BLAST19
Alternative sequenceiVSP_005835285 – 334GPSQN…GDYSN → D in isoform 3 and isoform 4. 4 PublicationsAdd BLAST50

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D55671 mRNA Translation: BAA09522.1
D55672 mRNA Translation: BAA09523.1
D55673 mRNA Translation: BAA09524.1
D55674 mRNA Translation: BAA09525.1
AF026126 Genomic DNA Translation: AAC23474.1
AF026126 Genomic DNA Translation: AAC23475.1
AF026126 Genomic DNA Translation: AAC23476.1
AK292707 mRNA Translation: BAF85396.1
AC124016 Genomic DNA Translation: AAY40913.1
CH471057 Genomic DNA Translation: EAX05874.1
BC002401 mRNA Translation: AAH02401.1
BC023977 mRNA Translation: AAH23977.1
BC026015 mRNA Translation: AAH26015.1
X03910 mRNA Translation: CAA27544.1 Sequence problems.
AF039575 mRNA Translation: AAB96683.1
M94630 mRNA Translation: AAA35781.1 Sequence problems.
CCDSiCCDS3590.1 [Q14103-3]
CCDS3591.1 [Q14103-2]
CCDS3592.1 [Q14103-1]
PIRiA24016
A44192
B48138
RefSeqiNP_001003810.1, NM_001003810.1 [Q14103-4]
NP_002129.2, NM_002138.3 [Q14103-3]
NP_112737.1, NM_031369.2 [Q14103-2]
NP_112738.1, NM_031370.2 [Q14103-1]
UniGeneiHs.480073

Genome annotation databases

EnsembliENST00000313899; ENSP00000313199; ENSG00000138668 [Q14103-1]
ENST00000352301; ENSP00000305860; ENSG00000138668 [Q14103-2]
ENST00000353341; ENSP00000313327; ENSG00000138668 [Q14103-3]
GeneIDi3184
KEGGihsa:3184
UCSCiuc003hmm.2 human [Q14103-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiHNRPD_HUMAN
AccessioniPrimary (citable) accession number: Q14103
Secondary accession number(s): A8K9J2
, P07029, Q01858, Q14100, Q14101, Q14102, Q4W5A1, Q9UCE8, Q9UCE9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1996
Last modified: June 20, 2018
This is version 210 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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