UniProtKB - Q13972 (RGRF1_HUMAN)
Protein
Ras-specific guanine nucleotide-releasing factor 1
Gene
RASGRF1
Organism
Homo sapiens (Human)
Status
Functioni
Promotes the exchange of Ras-bound GDP by GTP.1 Publication
GO - Molecular functioni
- glutamate receptor binding Source: Ensembl
- guanyl-nucleotide exchange factor activity Source: ProtInc
- Ras guanyl-nucleotide exchange factor activity Source: BHF-UCL
GO - Biological processi
- activation of GTPase activity Source: HGNC-UCL
- cell population proliferation Source: Ensembl
- long-term memory Source: UniProtKB
- MAPK cascade Source: Reactome
- neuron projection development Source: HGNC-UCL
- positive regulation of GTPase activity Source: HGNC-UCL
- positive regulation of Ras protein signal transduction Source: UniProtKB
- regulation of neuronal synaptic plasticity Source: Ensembl
- regulation of NMDA receptor activity Source: Ensembl
- regulation of Rac protein signal transduction Source: HGNC-UCL
- regulation of Ras protein signal transduction Source: HGNC-UCL
- regulation of Rho protein signal transduction Source: InterPro
- regulation of synaptic plasticity Source: UniProtKB
- response to endoplasmic reticulum stress Source: BHF-UCL
- signal transduction Source: ProtInc
- small GTPase mediated signal transduction Source: InterPro
Keywordsi
Molecular function | Guanine-nucleotide releasing factor |
Enzyme and pathway databases
PathwayCommonsi | Q13972 |
Reactomei | R-HSA-442982, Ras activation upon Ca2+ influx through NMDA receptor R-HSA-5673001, RAF/MAP kinase cascade |
SIGNORi | Q13972 |
Names & Taxonomyi
Protein namesi | Recommended name: Ras-specific guanine nucleotide-releasing factor 1Short name: Ras-GRF1 Alternative name(s): Guanine nucleotide-releasing protein Short name: GNRP Ras-specific nucleotide exchange factor CDC25 |
Gene namesi | Name:RASGRF1 Synonyms:CDC25, GNRP, GRF1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000058335.15 |
HGNCi | HGNC:9875, RASGRF1 |
MIMi | 606600, gene |
neXtProti | NX_Q13972 |
Subcellular locationi
Cytosol
- cytosol Source: HGNC-UCL
Plasma Membrane
- plasma membrane Source: Reactome
Other locations
- growth cone Source: HGNC-UCL
- neuron projection Source: UniProtKB
Pathology & Biotechi
Organism-specific databases
DisGeNETi | 5923 |
OpenTargetsi | ENSG00000058335 |
PharmGKBi | PA34238 |
Miscellaneous databases
Pharosi | Q13972, Tbio |
Chemistry databases
ChEMBLi | CHEMBL5468 |
Polymorphism and mutation databases
BioMutai | RASGRF1 |
DMDMi | 13124259 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000068880 | 1 – 1273 | Ras-specific guanine nucleotide-releasing factor 1Add BLAST | 1273 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 577 | Phosphoserine; by PLK2By similarity | 1 | |
Modified residuei | 626 | Phosphoserine; by PLK2By similarity | 1 | |
Modified residuei | 758 | PhosphoserineBy similarity | 1 | |
Modified residuei | 779 | Phosphoserine; by PLK2By similarity | 1 | |
Modified residuei | 800 | Phosphoserine; by PLK2By similarity | 1 |
Post-translational modificationi
Phosphorylated by PLK2, leading to ubiquitination and degradation by the proteasome.By similarity
Ubiquitinated and degraded following phosphorylation by PLK2.By similarity
Phosphorylated by SRC and LCK. Phosphorylation by LCK increases its capacity to stimulate the GDP/GTP exchange on Ras, whereas its phosphorylation by SRC seems not to have an effect on stimulation activity.1 Publication
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | Q13972 |
MassIVEi | Q13972 |
PaxDbi | Q13972 |
PeptideAtlasi | Q13972 |
PRIDEi | Q13972 |
ProteomicsDBi | 28264 59779 [Q13972-1] 59780 [Q13972-2] |
PTM databases
GlyConnecti | 2067, 1 N-Linked glycan (1 site) |
GlyGeni | Q13972, 1 site, 1 N-linked glycan (1 site) |
iPTMneti | Q13972 |
PhosphoSitePlusi | Q13972 |
Expressioni
Gene expression databases
Bgeei | ENSG00000058335, Expressed in C1 segment of cervical spinal cord and 132 other tissues |
Genevisiblei | Q13972, HS |
Organism-specific databases
HPAi | ENSG00000058335, Group enriched (brain, lung) |
Interactioni
Subunit structurei
Homooligomer and heterooligomer with RASGRF2.
Interacts with USP8, thereby regulating its stability (By similarity).
By similarityGO - Molecular functioni
- glutamate receptor binding Source: Ensembl
- guanyl-nucleotide exchange factor activity Source: ProtInc
- Ras guanyl-nucleotide exchange factor activity Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 111858, 11 interactors |
IntActi | Q13972, 8 interactors |
MINTi | Q13972 |
STRINGi | 9606.ENSP00000405963 |
Miscellaneous databases
RNActi | Q13972, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 22 – 129 | PH 1PROSITE-ProRule annotationAdd BLAST | 108 | |
Domaini | 204 – 229 | IQPROSITE-ProRule annotationAdd BLAST | 26 | |
Domaini | 240 – 426 | DHPROSITE-ProRule annotationAdd BLAST | 187 | |
Domaini | 467 – 584 | PH 2PROSITE-ProRule annotationAdd BLAST | 118 | |
Domaini | 644 – 761 | N-terminal Ras-GEFPROSITE-ProRule annotationAdd BLAST | 118 | |
Domaini | 1038 – 1270 | Ras-GEFPROSITE-ProRule annotationAdd BLAST | 233 |
Domaini
The DH (DBL-homology) domain mediates interaction with RASGRF2.
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG3417, Eukaryota |
GeneTreei | ENSGT00940000157599 |
HOGENOMi | CLU_003405_0_1_1 |
InParanoidi | Q13972 |
OMAi | HHYFTVN |
PhylomeDBi | Q13972 |
TreeFami | TF317296 |
Family and domain databases
CDDi | cd00155, RasGEF, 1 hit cd06224, REM, 1 hit cd00160, RhoGEF, 1 hit |
Gene3Di | 1.10.840.10, 1 hit 1.20.900.10, 1 hit 2.30.29.30, 2 hits |
InterProi | View protein in InterPro IPR035899, DBL_dom_sf IPR000219, DH-domain IPR001331, GDS_CDC24_CS IPR000048, IQ_motif_EF-hand-BS IPR011993, PH-like_dom_sf IPR001849, PH_domain IPR008937, Ras-like_GEF IPR000651, Ras-like_Gua-exchang_fac_N IPR019804, Ras_G-nucl-exch_fac_CS IPR023578, Ras_GEF_dom_sf IPR001895, RASGEF_cat_dom IPR036964, RASGEF_cat_dom_sf IPR030745, RasGRF1 |
PANTHERi | PTHR23113, PTHR23113, 1 hit PTHR23113:SF193, PTHR23113:SF193, 1 hit |
Pfami | View protein in Pfam PF00169, PH, 2 hits PF00617, RasGEF, 1 hit PF00618, RasGEF_N, 1 hit PF00621, RhoGEF, 1 hit |
SMARTi | View protein in SMART SM00233, PH, 2 hits SM00147, RasGEF, 1 hit SM00229, RasGEFN, 2 hits SM00325, RhoGEF, 1 hit |
SUPFAMi | SSF48065, SSF48065, 1 hit SSF48366, SSF48366, 1 hit |
PROSITEi | View protein in PROSITE PS00741, DH_1, 1 hit PS50010, DH_2, 1 hit PS50096, IQ, 1 hit PS50003, PH_DOMAIN, 2 hits PS00720, RASGEF, 1 hit PS50009, RASGEF_CAT, 1 hit PS50212, RASGEF_NTER, 1 hit |
s (3)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q13972-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MQKGIRLNDG HVASLGLLAR KDGTRKGYLS KRSSDNTKWQ TKWFALLQNL
60 70 80 90 100
LFYFESDSSS RPSGLYLLEG CVCDRAPSPK PALSAKEPLE KQHYFTVNFS
110 120 130 140 150
HENQKALELR TEDAKDCDEW VAAIAHASYR TLATEHEALM QKYLHLLQIV
160 170 180 190 200
ETEKTVAKQL RQQIEDGEIE IERLKAEITS LLKDNERIQS TQTVAPNDED
210 220 230 240 250
SDIKKIKKVQ SFLRGWLCRR KWKTIIQDYI RSPHADSMRK RNQVVFSMLE
260 270 280 290 300
AEAEYVQQLH ILVNNFLRPL RMAASSKKPP ITHDDVSSIF LNSETIMFLH
310 320 330 340 350
QIFYQGLKAR ISSWPTLVLA DLFDILLPML NIYQEFVRNH QYSLQILAHC
360 370 380 390 400
KQNRDFDKLL KHYEAKPDCE ERTLETFLTY PMFQIPRYIL TLHELLAHTP
410 420 430 440 450
HEHVERNSLD YAKSKLEELS RIMHDEVSET ENIRKNLAIE RMIIEGCEIL
460 470 480 490 500
LDTSQTFVRQ GSLIQVPMSE KGKITRGRLG SLSLKKEGER QCFLFSKHLI
510 520 530 540 550
ICTRGSGGKL HLTKNGVISL IDCTLLEEPE STEEEAKGSG QDIDHLDFKI
560 570 580 590 600
GVEPKDSPPF TVILVASSRQ EKAAWTSDIS QCVDNIRCNG LMMNAFEENS
610 620 630 640 650
KVTVPQMIKR TREGTREAEM SRSDASLYCD DVDIRFSKTM NSCKVLQIRY
660 670 680 690 700
ASVERLLERL TDLRFLSIDF LNTFLHSYRV FTTAIVVLDK LITIYKKPIS
710 720 730 740 750
AIPARWLRSL ELLFASGQNN KLLYGEPPKS PRATRKFSSP PPLSITKTSS
760 770 780 790 800
PSRRRKLSLN IPIITGGKAL DLAALSCNSN GYTSMYSAMS PFSKATLDTS
810 820 830 840 850
KLYVSSSFTN KIPDEGDTTP EKPEDPSALS KQSSEVSMRE ESDIDQNQSD
860 870 880 890 900
DGDTETSPTK SPTTPKSVKN KNSSEFPLFS YNNGVVMTSC RELDNNRSAL
910 920 930 940 950
SAASAFAIAT AGANEGTPNK EKYRRMSLAS AGFPPDQRNG DKEFVIRRAA
960 970 980 990 1000
TNRVLNVLRH WVSKHSQDFE TNDELKCKVI GFLEEVMHDP ELLTQERKAA
1010 1020 1030 1040 1050
ANIIRTLTQE DPGDNQITLE EITQMAEGVK AEPFENHSAL EIAEQLTLLD
1060 1070 1080 1090 1100
HLVFKKIPYE EFFGQGWMKL EKNERTPYIM KTTKHFNDIS NLIASEIIRN
1110 1120 1130 1140 1150
EDINARVSAI EKWVAVADIC RCLHNYNAVL EITSSMNRSA IFRLKKTWLK
1160 1170 1180 1190 1200
VSKQTKALID KLQKLVSSEG RFKNLREALK NCDPPCVPYL GMYLTDLAFI
1210 1220 1230 1240 1250
EEGTPNYTED GLVNFSKMRM ISHIIREIRQ FQQTAYKIEH QAKVTQYLLD
1260 1270
QSFVMDEESL YESSLRIEPK LPT
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 1053 | V → A in M91815 (PubMed:1379731).Curated | 1 | |
Sequence conflicti | 1111 | E → G in M91815 (PubMed:1379731).Curated | 1 | |
Sequence conflicti | 1134 | S → C in M91815 (PubMed:1379731).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_012032 | 1 – 784 | Missing in isoform 2. 1 PublicationAdd BLAST | 784 | |
Alternative sequenceiVSP_054072 | 610 – 622 | Missing in isoform 3. 1 PublicationAdd BLAST | 13 | |
Alternative sequenceiVSP_054073 | 705 – 707 | Missing in isoform 3. 1 Publication | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L26584 mRNA Translation: AAA58417.1 S62035 mRNA Translation: AAB26881.1 AC011944 Genomic DNA No translation available. AC104231 Genomic DNA No translation available. CH471136 Genomic DNA Translation: EAW99141.1 AK226101 mRNA No translation available. M91815 mRNA No translation available. |
CCDSi | CCDS10309.1 [Q13972-1] CCDS42065.1 [Q13972-2] CCDS45320.1 [Q13972-3] |
PIRi | A38985 |
RefSeqi | NP_002882.3, NM_002891.4 [Q13972-1] NP_722522.1, NM_153815.2 [Q13972-2] |
Genome annotation databases
Ensembli | ENST00000394745; ENSP00000378228; ENSG00000058335 [Q13972-2] ENST00000419573; ENSP00000405963; ENSG00000058335 [Q13972-1] ENST00000558480; ENSP00000452781; ENSG00000058335 [Q13972-3] |
GeneIDi | 5923 |
KEGGi | hsa:5923 |
UCSCi | uc002beo.4, human [Q13972-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L26584 mRNA Translation: AAA58417.1 S62035 mRNA Translation: AAB26881.1 AC011944 Genomic DNA No translation available. AC104231 Genomic DNA No translation available. CH471136 Genomic DNA Translation: EAW99141.1 AK226101 mRNA No translation available. M91815 mRNA No translation available. |
CCDSi | CCDS10309.1 [Q13972-1] CCDS42065.1 [Q13972-2] CCDS45320.1 [Q13972-3] |
PIRi | A38985 |
RefSeqi | NP_002882.3, NM_002891.4 [Q13972-1] NP_722522.1, NM_153815.2 [Q13972-2] |
3D structure databases
SMRi | Q13972 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 111858, 11 interactors |
IntActi | Q13972, 8 interactors |
MINTi | Q13972 |
STRINGi | 9606.ENSP00000405963 |
Chemistry databases
ChEMBLi | CHEMBL5468 |
PTM databases
GlyConnecti | 2067, 1 N-Linked glycan (1 site) |
GlyGeni | Q13972, 1 site, 1 N-linked glycan (1 site) |
iPTMneti | Q13972 |
PhosphoSitePlusi | Q13972 |
Polymorphism and mutation databases
BioMutai | RASGRF1 |
DMDMi | 13124259 |
Proteomic databases
jPOSTi | Q13972 |
MassIVEi | Q13972 |
PaxDbi | Q13972 |
PeptideAtlasi | Q13972 |
PRIDEi | Q13972 |
ProteomicsDBi | 28264 59779 [Q13972-1] 59780 [Q13972-2] |
Protocols and materials databases
Antibodypediai | 4170, 318 antibodies |
Genome annotation databases
Ensembli | ENST00000394745; ENSP00000378228; ENSG00000058335 [Q13972-2] ENST00000419573; ENSP00000405963; ENSG00000058335 [Q13972-1] ENST00000558480; ENSP00000452781; ENSG00000058335 [Q13972-3] |
GeneIDi | 5923 |
KEGGi | hsa:5923 |
UCSCi | uc002beo.4, human [Q13972-1] |
Organism-specific databases
CTDi | 5923 |
DisGeNETi | 5923 |
EuPathDBi | HostDB:ENSG00000058335.15 |
GeneCardsi | RASGRF1 |
HGNCi | HGNC:9875, RASGRF1 |
HPAi | ENSG00000058335, Group enriched (brain, lung) |
MIMi | 606600, gene |
neXtProti | NX_Q13972 |
OpenTargetsi | ENSG00000058335 |
PharmGKBi | PA34238 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3417, Eukaryota |
GeneTreei | ENSGT00940000157599 |
HOGENOMi | CLU_003405_0_1_1 |
InParanoidi | Q13972 |
OMAi | HHYFTVN |
PhylomeDBi | Q13972 |
TreeFami | TF317296 |
Enzyme and pathway databases
PathwayCommonsi | Q13972 |
Reactomei | R-HSA-442982, Ras activation upon Ca2+ influx through NMDA receptor R-HSA-5673001, RAF/MAP kinase cascade |
SIGNORi | Q13972 |
Miscellaneous databases
BioGRID-ORCSi | 5923, 4 hits in 845 CRISPR screens |
ChiTaRSi | RASGRF1, human |
GenomeRNAii | 5923 |
Pharosi | Q13972, Tbio |
PROi | PR:Q13972 |
RNActi | Q13972, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000058335, Expressed in C1 segment of cervical spinal cord and 132 other tissues |
Genevisiblei | Q13972, HS |
Family and domain databases
CDDi | cd00155, RasGEF, 1 hit cd06224, REM, 1 hit cd00160, RhoGEF, 1 hit |
Gene3Di | 1.10.840.10, 1 hit 1.20.900.10, 1 hit 2.30.29.30, 2 hits |
InterProi | View protein in InterPro IPR035899, DBL_dom_sf IPR000219, DH-domain IPR001331, GDS_CDC24_CS IPR000048, IQ_motif_EF-hand-BS IPR011993, PH-like_dom_sf IPR001849, PH_domain IPR008937, Ras-like_GEF IPR000651, Ras-like_Gua-exchang_fac_N IPR019804, Ras_G-nucl-exch_fac_CS IPR023578, Ras_GEF_dom_sf IPR001895, RASGEF_cat_dom IPR036964, RASGEF_cat_dom_sf IPR030745, RasGRF1 |
PANTHERi | PTHR23113, PTHR23113, 1 hit PTHR23113:SF193, PTHR23113:SF193, 1 hit |
Pfami | View protein in Pfam PF00169, PH, 2 hits PF00617, RasGEF, 1 hit PF00618, RasGEF_N, 1 hit PF00621, RhoGEF, 1 hit |
SMARTi | View protein in SMART SM00233, PH, 2 hits SM00147, RasGEF, 1 hit SM00229, RasGEFN, 2 hits SM00325, RhoGEF, 1 hit |
SUPFAMi | SSF48065, SSF48065, 1 hit SSF48366, SSF48366, 1 hit |
PROSITEi | View protein in PROSITE PS00741, DH_1, 1 hit PS50010, DH_2, 1 hit PS50096, IQ, 1 hit PS50003, PH_DOMAIN, 2 hits PS00720, RASGEF, 1 hit PS50009, RASGEF_CAT, 1 hit PS50212, RASGEF_NTER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RGRF1_HUMAN | |
Accessioni | Q13972Primary (citable) accession number: Q13972 Secondary accession number(s): F8VPA5 Q16027 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 21, 2001 |
Last sequence update: | April 16, 2014 | |
Last modified: | December 2, 2020 | |
This is version 196 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - Human chromosome 15
Human chromosome 15: entries, gene names and cross-references to MIM