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UniProtKB - Q13972 (RGRF1_HUMAN)

Protein

Ras-specific guanine nucleotide-releasing factor 1

Gene

RASGRF1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Promotes the exchange of Ras-bound GDP by GTP.1 Publication

GO - Molecular functioni

  • glutamate receptor binding Source: Ensembl
  • guanyl-nucleotide exchange factor activity Source: ProtInc
  • Ras guanyl-nucleotide exchange factor activity Source: BHF-UCL
  • Rho guanyl-nucleotide exchange factor activity Source: InterPro
View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionGuanine-nucleotide releasing factor

Enzyme and pathway databases

ReactomeiR-HSA-442982 Ras activation upon Ca2+ influx through NMDA receptor
R-HSA-5673001 RAF/MAP kinase cascade
SIGNORiQ13972

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-specific guanine nucleotide-releasing factor 1
Short name:
Ras-GRF1
Alternative name(s):
Guanine nucleotide-releasing protein
Short name:
GNRP
Ras-specific nucleotide exchange factor CDC25
Gene namesi
Name:RASGRF1
Synonyms:CDC25, GNRP, GRF1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

EuPathDBiHostDB:ENSG00000058335.15
HGNCiHGNC:9875 RASGRF1
MIMi606600 gene
neXtProtiNX_Q13972

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

DisGeNETi5923
OpenTargetsiENSG00000058335
PharmGKBiPA34238

Chemistry databases

ChEMBLiCHEMBL5468

Polymorphism and mutation databases

DMDMi13124259

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000688801 – 1273Ras-specific guanine nucleotide-releasing factor 1Add BLAST1273

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei577Phosphoserine; by PLK2By similarity1
Modified residuei626Phosphoserine; by PLK2By similarity1
Modified residuei758PhosphoserineBy similarity1
Modified residuei779Phosphoserine; by PLK2By similarity1
Modified residuei800Phosphoserine; by PLK2By similarity1

Post-translational modificationi

Phosphorylated by PLK2, leading to ubiquitination and degradation by the proteasome.By similarity
Ubiquitinated and degraded following phosphorylation by PLK2.By similarity
Phosphorylated by SRC and LCK. Phosphorylation by LCK increases its capacity to stimulate the GDP/GTP exchange on Ras, whereas its phosphorylation by SRC seems not to have an effect on stimulation activity.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ13972
PeptideAtlasiQ13972
PRIDEiQ13972
ProteomicsDBi59779
59780 [Q13972-2]

PTM databases

iPTMnetiQ13972
PhosphoSitePlusiQ13972

Expressioni

Gene expression databases

BgeeiENSG00000058335 Expressed in 112 organ(s), highest expression level in C1 segment of cervical spinal cord
CleanExiHS_RASGRF1
GenevisibleiQ13972 HS

Organism-specific databases

HPAiCAB010413
HPA041965

Interactioni

Subunit structurei

Homooligomer and heterooligomer with RASGRF2. Interacts with USP8, thereby regulating its stability (By similarity).By similarity

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridi111858, 11 interactors
IntActiQ13972, 7 interactors
MINTiQ13972
STRINGi9606.ENSP00000405963

Structurei

3D structure databases

ProteinModelPortaliQ13972
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 129PH 1PROSITE-ProRule annotationAdd BLAST108
Domaini204 – 229IQPROSITE-ProRule annotationAdd BLAST26
Domaini240 – 426DHPROSITE-ProRule annotationAdd BLAST187
Domaini467 – 584PH 2PROSITE-ProRule annotationAdd BLAST118
Domaini644 – 761N-terminal Ras-GEFPROSITE-ProRule annotationAdd BLAST118
Domaini1038 – 1270Ras-GEFPROSITE-ProRule annotationAdd BLAST233

Domaini

The DH (DBL-homology) domain mediates interaction with RASGRF2.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IQ6Q Eukaryota
ENOG410XPWA LUCA
GeneTreeiENSGT00910000143985
HOGENOMiHOG000046000
HOVERGENiHBG005208
InParanoidiQ13972
KOiK04349
OMAiHASYRNL
OrthoDBiEOG091G09V6
PhylomeDBiQ13972
TreeFamiTF317296

Family and domain databases

CDDicd00155 RasGEF, 1 hit
cd06224 REM, 1 hit
cd00160 RhoGEF, 1 hit
Gene3Di1.10.840.10, 1 hit
1.20.900.10, 1 hit
2.30.29.30, 2 hits
InterProiView protein in InterPro
IPR035899 DBL_dom_sf
IPR000219 DH-domain
IPR001331 GDS_CDC24_CS
IPR000048 IQ_motif_EF-hand-BS
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR008937 Ras-like_GEF
IPR000651 Ras-like_Gua-exchang_fac_N
IPR019804 Ras_G-nucl-exch_fac_CS
IPR023578 Ras_GEF_dom_sf
IPR001895 RASGEF_cat_dom
IPR036964 RASGEF_cat_dom_sf
IPR030745 RasGRF1
PANTHERiPTHR23113 PTHR23113, 1 hit
PTHR23113:SF193 PTHR23113:SF193, 1 hit
PfamiView protein in Pfam
PF00169 PH, 2 hits
PF00617 RasGEF, 1 hit
PF00618 RasGEF_N, 1 hit
PF00621 RhoGEF, 1 hit
SMARTiView protein in SMART
SM00233 PH, 2 hits
SM00147 RasGEF, 1 hit
SM00229 RasGEFN, 2 hits
SM00325 RhoGEF, 1 hit
SUPFAMiSSF48065 SSF48065, 1 hit
SSF48366 SSF48366, 1 hit
PROSITEiView protein in PROSITE
PS00741 DH_1, 1 hit
PS50010 DH_2, 1 hit
PS50096 IQ, 1 hit
PS50003 PH_DOMAIN, 2 hits
PS00720 RASGEF, 1 hit
PS50009 RASGEF_CAT, 1 hit
PS50212 RASGEF_NTER, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q13972-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MQKGIRLNDG HVASLGLLAR KDGTRKGYLS KRSSDNTKWQ TKWFALLQNL 
        60         70         80         90        100
LFYFESDSSS RPSGLYLLEG CVCDRAPSPK PALSAKEPLE KQHYFTVNFS 
       110        120        130        140        150
HENQKALELR TEDAKDCDEW VAAIAHASYR TLATEHEALM QKYLHLLQIV 
       160        170        180        190        200
ETEKTVAKQL RQQIEDGEIE IERLKAEITS LLKDNERIQS TQTVAPNDED 
       210        220        230        240        250
SDIKKIKKVQ SFLRGWLCRR KWKTIIQDYI RSPHADSMRK RNQVVFSMLE 
       260        270        280        290        300
AEAEYVQQLH ILVNNFLRPL RMAASSKKPP ITHDDVSSIF LNSETIMFLH 
       310        320        330        340        350
QIFYQGLKAR ISSWPTLVLA DLFDILLPML NIYQEFVRNH QYSLQILAHC 
       360        370        380        390        400
KQNRDFDKLL KHYEAKPDCE ERTLETFLTY PMFQIPRYIL TLHELLAHTP 
       410        420        430        440        450
HEHVERNSLD YAKSKLEELS RIMHDEVSET ENIRKNLAIE RMIIEGCEIL 
       460        470        480        490        500
LDTSQTFVRQ GSLIQVPMSE KGKITRGRLG SLSLKKEGER QCFLFSKHLI 
       510        520        530        540        550
ICTRGSGGKL HLTKNGVISL IDCTLLEEPE STEEEAKGSG QDIDHLDFKI 
       560        570        580        590        600
GVEPKDSPPF TVILVASSRQ EKAAWTSDIS QCVDNIRCNG LMMNAFEENS 
       610        620        630        640        650
KVTVPQMIKR TREGTREAEM SRSDASLYCD DVDIRFSKTM NSCKVLQIRY 
       660        670        680        690        700
ASVERLLERL TDLRFLSIDF LNTFLHSYRV FTTAIVVLDK LITIYKKPIS 
       710        720        730        740        750
AIPARWLRSL ELLFASGQNN KLLYGEPPKS PRATRKFSSP PPLSITKTSS 
       760        770        780        790        800
PSRRRKLSLN IPIITGGKAL DLAALSCNSN GYTSMYSAMS PFSKATLDTS 
       810        820        830        840        850
KLYVSSSFTN KIPDEGDTTP EKPEDPSALS KQSSEVSMRE ESDIDQNQSD 
       860        870        880        890        900
DGDTETSPTK SPTTPKSVKN KNSSEFPLFS YNNGVVMTSC RELDNNRSAL 
       910        920        930        940        950
SAASAFAIAT AGANEGTPNK EKYRRMSLAS AGFPPDQRNG DKEFVIRRAA 
       960        970        980        990       1000
TNRVLNVLRH WVSKHSQDFE TNDELKCKVI GFLEEVMHDP ELLTQERKAA 
      1010       1020       1030       1040       1050
ANIIRTLTQE DPGDNQITLE EITQMAEGVK AEPFENHSAL EIAEQLTLLD 
      1060       1070       1080       1090       1100
HLVFKKIPYE EFFGQGWMKL EKNERTPYIM KTTKHFNDIS NLIASEIIRN 
      1110       1120       1130       1140       1150
EDINARVSAI EKWVAVADIC RCLHNYNAVL EITSSMNRSA IFRLKKTWLK 
      1160       1170       1180       1190       1200
VSKQTKALID KLQKLVSSEG RFKNLREALK NCDPPCVPYL GMYLTDLAFI 
      1210       1220       1230       1240       1250
EEGTPNYTED GLVNFSKMRM ISHIIREIRQ FQQTAYKIEH QAKVTQYLLD 
      1260       1270 
QSFVMDEESL YESSLRIEPK LPT
Length:1,273
Mass (Da):145,234
Last modified:April 16, 2014 - v2
Checksum:iB629AEAFDC7E500A
GO
Isoform 2 (identifier: Q13972-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-784: Missing.

Show »
Length:489
Mass (Da):55,489
Checksum:iDDF1EDAD9793F3EF
GO
Isoform 3 (identifier: Q13972-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     610-622: Missing.
     705-707: Missing.

Show »
Length:1,257
Mass (Da):143,218
Checksum:i097E066E032DFFDB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1053V → A in M91815 (PubMed:1379731).Curated1
Sequence conflicti1111E → G in M91815 (PubMed:1379731).Curated1
Sequence conflicti1134S → C in M91815 (PubMed:1379731).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0120321 – 784Missing in isoform 2. 1 PublicationAdd BLAST784
Alternative sequenceiVSP_054072610 – 622Missing in isoform 3. 1 PublicationAdd BLAST13
Alternative sequenceiVSP_054073705 – 707Missing in isoform 3. 1 Publication3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L26584 mRNA Translation: AAA58417.1
S62035 mRNA Translation: AAB26881.1
AC011944 Genomic DNA No translation available.
AC104231 Genomic DNA No translation available.
CH471136 Genomic DNA Translation: EAW99141.1
AK226101 mRNA No translation available.
M91815 mRNA No translation available.
CCDSiCCDS10309.1 [Q13972-1]
CCDS42065.1 [Q13972-2]
CCDS45320.1 [Q13972-3]
PIRiA38985
RefSeqiNP_002882.3, NM_002891.4 [Q13972-1]
NP_722522.1, NM_153815.2 [Q13972-2]
UniGeneiHs.459035

Genome annotation databases

EnsembliENST00000394745; ENSP00000378228; ENSG00000058335 [Q13972-2]
ENST00000419573; ENSP00000405963; ENSG00000058335 [Q13972-1]
ENST00000558480; ENSP00000452781; ENSG00000058335 [Q13972-3]
GeneIDi5923
KEGGihsa:5923
UCSCiuc002beo.4 human [Q13972-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L26584 mRNA Translation: AAA58417.1
S62035 mRNA Translation: AAB26881.1
AC011944 Genomic DNA No translation available.
AC104231 Genomic DNA No translation available.
CH471136 Genomic DNA Translation: EAW99141.1
AK226101 mRNA No translation available.
M91815 mRNA No translation available.
CCDSiCCDS10309.1 [Q13972-1]
CCDS42065.1 [Q13972-2]
CCDS45320.1 [Q13972-3]
PIRiA38985
RefSeqiNP_002882.3, NM_002891.4 [Q13972-1]
NP_722522.1, NM_153815.2 [Q13972-2]
UniGeneiHs.459035

3D structure databases

ProteinModelPortaliQ13972
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111858, 11 interactors
IntActiQ13972, 7 interactors
MINTiQ13972
STRINGi9606.ENSP00000405963

Chemistry databases

ChEMBLiCHEMBL5468

PTM databases

iPTMnetiQ13972
PhosphoSitePlusiQ13972

Polymorphism and mutation databases

DMDMi13124259

Proteomic databases

PaxDbiQ13972
PeptideAtlasiQ13972
PRIDEiQ13972
ProteomicsDBi59779
59780 [Q13972-2]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000394745; ENSP00000378228; ENSG00000058335 [Q13972-2]
ENST00000419573; ENSP00000405963; ENSG00000058335 [Q13972-1]
ENST00000558480; ENSP00000452781; ENSG00000058335 [Q13972-3]
GeneIDi5923
KEGGihsa:5923
UCSCiuc002beo.4 human [Q13972-1]

Organism-specific databases

CTDi5923
DisGeNETi5923
EuPathDBiHostDB:ENSG00000058335.15
GeneCardsiRASGRF1
H-InvDBiHIX0023098
HGNCiHGNC:9875 RASGRF1
HPAiCAB010413
HPA041965
MIMi606600 gene
neXtProtiNX_Q13972
OpenTargetsiENSG00000058335
PharmGKBiPA34238
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IQ6Q Eukaryota
ENOG410XPWA LUCA
GeneTreeiENSGT00910000143985
HOGENOMiHOG000046000
HOVERGENiHBG005208
InParanoidiQ13972
KOiK04349
OMAiHASYRNL
OrthoDBiEOG091G09V6
PhylomeDBiQ13972
TreeFamiTF317296

Enzyme and pathway databases

ReactomeiR-HSA-442982 Ras activation upon Ca2+ influx through NMDA receptor
R-HSA-5673001 RAF/MAP kinase cascade
SIGNORiQ13972

Miscellaneous databases

ChiTaRSiRASGRF1 human
GenomeRNAii5923
PROiPR:Q13972
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000058335 Expressed in 112 organ(s), highest expression level in C1 segment of cervical spinal cord
CleanExiHS_RASGRF1
GenevisibleiQ13972 HS

Family and domain databases

CDDicd00155 RasGEF, 1 hit
cd06224 REM, 1 hit
cd00160 RhoGEF, 1 hit
Gene3Di1.10.840.10, 1 hit
1.20.900.10, 1 hit
2.30.29.30, 2 hits
InterProiView protein in InterPro
IPR035899 DBL_dom_sf
IPR000219 DH-domain
IPR001331 GDS_CDC24_CS
IPR000048 IQ_motif_EF-hand-BS
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR008937 Ras-like_GEF
IPR000651 Ras-like_Gua-exchang_fac_N
IPR019804 Ras_G-nucl-exch_fac_CS
IPR023578 Ras_GEF_dom_sf
IPR001895 RASGEF_cat_dom
IPR036964 RASGEF_cat_dom_sf
IPR030745 RasGRF1
PANTHERiPTHR23113 PTHR23113, 1 hit
PTHR23113:SF193 PTHR23113:SF193, 1 hit
PfamiView protein in Pfam
PF00169 PH, 2 hits
PF00617 RasGEF, 1 hit
PF00618 RasGEF_N, 1 hit
PF00621 RhoGEF, 1 hit
SMARTiView protein in SMART
SM00233 PH, 2 hits
SM00147 RasGEF, 1 hit
SM00229 RasGEFN, 2 hits
SM00325 RhoGEF, 1 hit
SUPFAMiSSF48065 SSF48065, 1 hit
SSF48366 SSF48366, 1 hit
PROSITEiView protein in PROSITE
PS00741 DH_1, 1 hit
PS50010 DH_2, 1 hit
PS50096 IQ, 1 hit
PS50003 PH_DOMAIN, 2 hits
PS00720 RASGEF, 1 hit
PS50009 RASGEF_CAT, 1 hit
PS50212 RASGEF_NTER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRGRF1_HUMAN
AccessioniPrimary (citable) accession number: Q13972
Secondary accession number(s): F8VPA5
, H0YKF2, J3KQP9, Q16027
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: April 16, 2014
Last modified: November 7, 2018
This is version 181 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  2. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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