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Protein

General transcription factor IIH subunit 3

Gene

GTF2H3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri268 – 285C4-typeAdd BLAST18

GO - Molecular functioni

  • damaged DNA binding Source: ProtInc
  • DNA-binding transcription factor activity Source: ProtInc
  • metal ion binding Source: UniProtKB-KW
  • protein N-terminus binding Source: UniProtKB
  • translation factor activity, RNA binding Source: ProtInc

GO - Biological processi

Keywordsi

Biological processDNA damage, DNA repair, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-112382 Formation of RNA Pol II elongation complex
R-HSA-113418 Formation of the Early Elongation Complex
R-HSA-167152 Formation of HIV elongation complex in the absence of HIV Tat
R-HSA-167158 Formation of the HIV-1 Early Elongation Complex
R-HSA-167160 RNA Pol II CTD phosphorylation and interaction with CE during HIV infection
R-HSA-167161 HIV Transcription Initiation
R-HSA-167162 RNA Polymerase II HIV Promoter Escape
R-HSA-167172 Transcription of the HIV genome
R-HSA-167200 Formation of HIV-1 elongation complex containing HIV-1 Tat
R-HSA-167246 Tat-mediated elongation of the HIV-1 transcript
R-HSA-427413 NoRC negatively regulates rRNA expression
R-HSA-5696395 Formation of Incision Complex in GG-NER
R-HSA-5696400 Dual Incision in GG-NER
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6781823 Formation of TC-NER Pre-Incision Complex
R-HSA-6781827 Transcription-Coupled Nucleotide Excision Repair (TC-NER)
R-HSA-6782135 Dual incision in TC-NER
R-HSA-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-HSA-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-HSA-72086 mRNA Capping
R-HSA-73762 RNA Polymerase I Transcription Initiation
R-HSA-73772 RNA Polymerase I Promoter Escape
R-HSA-73776 RNA Polymerase II Promoter Escape
R-HSA-73777 RNA Polymerase I Chain Elongation
R-HSA-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-HSA-73863 RNA Polymerase I Transcription Termination
R-HSA-75953 RNA Polymerase II Transcription Initiation
R-HSA-75955 RNA Polymerase II Transcription Elongation
R-HSA-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-HSA-77075 RNA Pol II CTD phosphorylation and interaction with CE

Names & Taxonomyi

Protein namesi
Recommended name:
General transcription factor IIH subunit 3
Alternative name(s):
Basic transcription factor 2 34 kDa subunit
Short name:
BTF2 p341 Publication
General transcription factor IIH polypeptide 3
TFIIH basal transcription factor complex p34 subunit1 Publication
Gene namesi
Name:GTF2H3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000111358.13
HGNCiHGNC:4657 GTF2H3
MIMi601750 gene
neXtProtiNX_Q13889

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi2967
OpenTargetsiENSG00000111358
PharmGKBiPA29043

Polymorphism and mutation databases

DMDMi50403772

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001192511 – 308General transcription factor IIH subunit 3Add BLAST308

Proteomic databases

EPDiQ13889
MaxQBiQ13889
PaxDbiQ13889
PeptideAtlasiQ13889
PRIDEiQ13889
ProteomicsDBi59717

PTM databases

iPTMnetiQ13889
PhosphoSitePlusiQ13889

Expressioni

Gene expression databases

BgeeiENSG00000111358 Expressed in 234 organ(s), highest expression level in epithelium of mammary gland
CleanExiHS_GTF2H3
ExpressionAtlasiQ13889 baseline and differential
GenevisibleiQ13889 HS

Organism-specific databases

HPAiHPA004844
HPA053562

Interactioni

Subunit structurei

Part of a TFIID-containing RNA polymerase II pre-initiation complex that is composed of TBP and at least GTF2A1, GTF2A2, GTF2E1, GTF2E2, GTF2F1, GTF2H2, GTF2H3, GTF2H4, GTF2H5, GTF2B, TCEA1, ERCC2, ERCC3, TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed:27193682). Component of the 7-subunit TFIIH core complex composed of XPB/ERCC3, XPD/ERCC2, GTF2H1, GTF2H2, GTF2H3, GTF2H4 and GTF2H5, which is active in NER. The core complex associates with the 3-subunit CDK-activating kinase (CAK) module composed of CCNH/cyclin H, CDK7 and MNAT1 to form the 10-subunit holoenzyme (holo-TFIIH) active in transcription (PubMed:9852112). Interacts with RARA; the interaction requires prior phosphorylation of RARA on 'Ser-369' which then enhances interaction of RARA with CDK7 (By similarity).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
GTF2H2C_2Q6P1K83EBI-6380459,EBI-8469755

GO - Molecular functioni

Protein-protein interaction databases

BioGridi109222, 20 interactors
CORUMiQ13889
DIPiDIP-787N
IntActiQ13889, 7 interactors
STRINGi9606.ENSP00000445162

Structurei

Secondary structure

1308
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ13889
SMRiQ13889
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TFB4 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri268 – 285C4-typeAdd BLAST18

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2487 Eukaryota
COG5242 LUCA
GeneTreeiENSGT00390000013143
HOGENOMiHOG000175900
HOVERGENiHBG054271
InParanoidiQ13889
KOiK03143
OMAiFGNAHLM
OrthoDBiEOG091G0GIC
PhylomeDBiQ13889
TreeFamiTF314336

Family and domain databases

Gene3Di3.40.50.410, 1 hit
InterProiView protein in InterPro
IPR004600 TFIIH_Tfb4/GTF2H3
IPR036465 vWFA_dom_sf
PANTHERiPTHR12831 PTHR12831, 1 hit
PfamiView protein in Pfam
PF03850 Tfb4, 1 hit
TIGRFAMsiTIGR00627 tfb4, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 8 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q13889-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MVSDEDELNL LVIVVDANPI WWGKQALKES QFTLSKCIDA VMVLGNSHLF
60 70 80 90 100
MNRSNKLAVI ASHIQESRFL YPGKNGRLGD FFGDPGNPPE FNPSGSKDGK
110 120 130 140 150
YELLTSANEV IVEEIKDLMT KSDIKGQHTE TLLAGSLAKA LCYIHRMNKE
160 170 180 190 200
VKDNQEMKSR ILVIKAAEDS ALQYMNFMNV IFAAQKQNIL IDACVLDSDS
210 220 230 240 250
GLLQQACDIT GGLYLKVPQM PSLLQYLLWV FLPDQDQRSQ LILPPPVHVD
260 270 280 290 300
YRAACFCHRN LIEIGYVCSV CLSIFCNFSP ICTTCETAFK ISLPPVLKAK

KKKLKVSA
Length:308
Mass (Da):34,378
Last modified:July 19, 2004 - v2
Checksum:iE739E2176CD6BAA5
GO
Isoform 2 (identifier: Q13889-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-41: Missing.

Note: No experimental confirmation available.
Show »
Length:267
Mass (Da):29,777
Checksum:i9415C0930FAADD34
GO

Computationally mapped potential isoform sequencesi

There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F5GXG6F5GXG6_HUMAN
General transcription factor IIH su...
GTF2H3
177Annotation score:
F5GWD3F5GWD3_HUMAN
General transcription factor IIH su...
GTF2H3
258Annotation score:
F5H4K7F5H4K7_HUMAN
General transcription factor IIH su...
GTF2H3
210Annotation score:
A0A087WYD5A0A087WYD5_HUMAN
General transcription factor IIH su...
GTF2H3
162Annotation score:
F5GX35F5GX35_HUMAN
General transcription factor IIH su...
GTF2H3
200Annotation score:
H0YFF7H0YFF7_HUMAN
General transcription factor IIH su...
GTF2H3
194Annotation score:
F5H6X0F5H6X0_HUMAN
General transcription factor IIH su...
GTF2H3
148Annotation score:
F5H3G1F5H3G1_HUMAN
General transcription factor IIH su...
GTF2H3
67Annotation score:

Sequence cautioni

The sequence CAA82909 differs from that shown. Reason: Frameshift at position 302.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0551531 – 41Missing in isoform 2. 1 PublicationAdd BLAST41

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK298128 mRNA Translation: BAG60408.1
AK313200 mRNA Translation: BAG36016.1
AF548661 Genomic DNA Translation: AAN40702.1
AC117503 Genomic DNA No translation available.
CH471054 Genomic DNA Translation: EAW98426.1
BC039726 mRNA Translation: AAH39726.1
BC047868 mRNA Translation: AAH47868.2
BC065250 mRNA Translation: AAH65250.1
Z30093 mRNA Translation: CAA82909.1 Frameshift.
CCDSiCCDS61275.1 [Q13889-2]
CCDS9252.1 [Q13889-1]
PIRiS44455
RefSeqiNP_001258795.1, NM_001271866.1
NP_001258796.1, NM_001271867.1 [Q13889-2]
NP_001258797.1, NM_001271868.1
NP_001507.2, NM_001516.4 [Q13889-1]
UniGeneiHs.355348

Genome annotation databases

EnsembliENST00000228955; ENSP00000228955; ENSG00000111358 [Q13889-2]
ENST00000543341; ENSP00000445162; ENSG00000111358 [Q13889-1]
GeneIDi2967
KEGGihsa:2967
UCSCiuc001ufo.3 human [Q13889-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK298128 mRNA Translation: BAG60408.1
AK313200 mRNA Translation: BAG36016.1
AF548661 Genomic DNA Translation: AAN40702.1
AC117503 Genomic DNA No translation available.
CH471054 Genomic DNA Translation: EAW98426.1
BC039726 mRNA Translation: AAH39726.1
BC047868 mRNA Translation: AAH47868.2
BC065250 mRNA Translation: AAH65250.1
Z30093 mRNA Translation: CAA82909.1 Frameshift.
CCDSiCCDS61275.1 [Q13889-2]
CCDS9252.1 [Q13889-1]
PIRiS44455
RefSeqiNP_001258795.1, NM_001271866.1
NP_001258796.1, NM_001271867.1 [Q13889-2]
NP_001258797.1, NM_001271868.1
NP_001507.2, NM_001516.4 [Q13889-1]
UniGeneiHs.355348

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5IVWelectron microscopy10.0031-308[»]
5IY6electron microscopy7.2031-308[»]
5IY7electron microscopy8.6031-308[»]
5IY8electron microscopy7.9031-308[»]
5IY9electron microscopy6.3031-308[»]
5O85X-ray3.40A/C1-308[»]
5OF4electron microscopy4.40F1-308[»]
ProteinModelPortaliQ13889
SMRiQ13889
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109222, 20 interactors
CORUMiQ13889
DIPiDIP-787N
IntActiQ13889, 7 interactors
STRINGi9606.ENSP00000445162

PTM databases

iPTMnetiQ13889
PhosphoSitePlusiQ13889

Polymorphism and mutation databases

DMDMi50403772

Proteomic databases

EPDiQ13889
MaxQBiQ13889
PaxDbiQ13889
PeptideAtlasiQ13889
PRIDEiQ13889
ProteomicsDBi59717

Protocols and materials databases

DNASUi2967
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000228955; ENSP00000228955; ENSG00000111358 [Q13889-2]
ENST00000543341; ENSP00000445162; ENSG00000111358 [Q13889-1]
GeneIDi2967
KEGGihsa:2967
UCSCiuc001ufo.3 human [Q13889-1]

Organism-specific databases

CTDi2967
DisGeNETi2967
EuPathDBiHostDB:ENSG00000111358.13
GeneCardsiGTF2H3
H-InvDBiHIX0026378
HGNCiHGNC:4657 GTF2H3
HPAiHPA004844
HPA053562
MIMi601750 gene
neXtProtiNX_Q13889
OpenTargetsiENSG00000111358
PharmGKBiPA29043
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2487 Eukaryota
COG5242 LUCA
GeneTreeiENSGT00390000013143
HOGENOMiHOG000175900
HOVERGENiHBG054271
InParanoidiQ13889
KOiK03143
OMAiFGNAHLM
OrthoDBiEOG091G0GIC
PhylomeDBiQ13889
TreeFamiTF314336

Enzyme and pathway databases

ReactomeiR-HSA-112382 Formation of RNA Pol II elongation complex
R-HSA-113418 Formation of the Early Elongation Complex
R-HSA-167152 Formation of HIV elongation complex in the absence of HIV Tat
R-HSA-167158 Formation of the HIV-1 Early Elongation Complex
R-HSA-167160 RNA Pol II CTD phosphorylation and interaction with CE during HIV infection
R-HSA-167161 HIV Transcription Initiation
R-HSA-167162 RNA Polymerase II HIV Promoter Escape
R-HSA-167172 Transcription of the HIV genome
R-HSA-167200 Formation of HIV-1 elongation complex containing HIV-1 Tat
R-HSA-167246 Tat-mediated elongation of the HIV-1 transcript
R-HSA-427413 NoRC negatively regulates rRNA expression
R-HSA-5696395 Formation of Incision Complex in GG-NER
R-HSA-5696400 Dual Incision in GG-NER
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6781823 Formation of TC-NER Pre-Incision Complex
R-HSA-6781827 Transcription-Coupled Nucleotide Excision Repair (TC-NER)
R-HSA-6782135 Dual incision in TC-NER
R-HSA-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-HSA-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-HSA-72086 mRNA Capping
R-HSA-73762 RNA Polymerase I Transcription Initiation
R-HSA-73772 RNA Polymerase I Promoter Escape
R-HSA-73776 RNA Polymerase II Promoter Escape
R-HSA-73777 RNA Polymerase I Chain Elongation
R-HSA-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-HSA-73863 RNA Polymerase I Transcription Termination
R-HSA-75953 RNA Polymerase II Transcription Initiation
R-HSA-75955 RNA Polymerase II Transcription Elongation
R-HSA-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-HSA-77075 RNA Pol II CTD phosphorylation and interaction with CE

Miscellaneous databases

ChiTaRSiGTF2H3 human
GenomeRNAii2967
PROiPR:Q13889
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000111358 Expressed in 234 organ(s), highest expression level in epithelium of mammary gland
CleanExiHS_GTF2H3
ExpressionAtlasiQ13889 baseline and differential
GenevisibleiQ13889 HS

Family and domain databases

Gene3Di3.40.50.410, 1 hit
InterProiView protein in InterPro
IPR004600 TFIIH_Tfb4/GTF2H3
IPR036465 vWFA_dom_sf
PANTHERiPTHR12831 PTHR12831, 1 hit
PfamiView protein in Pfam
PF03850 Tfb4, 1 hit
TIGRFAMsiTIGR00627 tfb4, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTF2H3_HUMAN
AccessioniPrimary (citable) accession number: Q13889
Secondary accession number(s): B2R819
, B4DNZ6, Q7L0G0, Q96AT7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: July 19, 2004
Last modified: September 12, 2018
This is version 164 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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