UniProtKB - Q13882 (PTK6_HUMAN)
Protein-tyrosine kinase 6
PTK6
Functioni
Miscellaneous
Catalytic activityi
- ATP + L-tyrosyl-[protein] = ADP + H+ + O-phospho-L-tyrosyl-[protein]PROSITE-ProRule annotation3 PublicationsEC:2.7.10.2PROSITE-ProRule annotation3 Publications
Activity regulationi
Kineticsi
- KM=83 µM for ATP1 Publication
- Vmax=37 nmol/min/mg enzyme1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 219 | ATPPROSITE-ProRule annotation2 Publications | 1 | |
Active sitei | 312 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 197 – 205 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- identical protein binding Source: IntAct
- non-membrane spanning protein tyrosine kinase activity Source: UniProtKB
- protein tyrosine kinase activity Source: Reactome
- signaling receptor binding Source: GO_Central
GO - Biological processi
- cell differentiation Source: GO_Central
- cell migration Source: UniProtKB
- cellular response to retinoic acid Source: BHF-UCL
- ERBB2 signaling pathway Source: Reactome
- innate immune response Source: GO_Central
- intestinal epithelial cell differentiation Source: Ensembl
- negative regulation of growth Source: Ensembl
- negative regulation of protein tyrosine kinase activity Source: UniProtKB
- negative regulation of signal transduction Source: Reactome
- peptidyl-tyrosine autophosphorylation Source: GO_Central
- positive regulation of cell cycle Source: Reactome
- positive regulation of epidermal growth factor receptor signaling pathway Source: Reactome
- positive regulation of neuron projection development Source: BHF-UCL
- positive regulation of tyrosine phosphorylation of STAT protein Source: Reactome
- protein autophosphorylation Source: UniProtKB
- protein phosphorylation Source: ProtInc
- regulation of cell population proliferation Source: GO_Central
- transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central
- tyrosine phosphorylation of STAT protein Source: UniProtKB
Keywordsi
Molecular function | Kinase, Transferase, Tyrosine-protein kinase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.10.2, 2681 |
PathwayCommonsi | Q13882 |
Reactomei | R-HSA-187577, SCF(Skp2)-mediated degradation of p27/p21 R-HSA-69231, Cyclin D associated events in G1 R-HSA-8847993, ERBB2 Activates PTK6 Signaling R-HSA-8849468, PTK6 Regulates Proteins Involved in RNA Processing R-HSA-8849469, PTK6 Regulates RTKs and Their Effectors AKT1 and DOK1 R-HSA-8849470, PTK6 Regulates Cell Cycle R-HSA-8849471, PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases R-HSA-8849472, PTK6 Down-Regulation R-HSA-8849473, PTK6 Expression R-HSA-8849474, PTK6 Activates STAT3 R-HSA-8857538, PTK6 promotes HIF1A stabilization |
SignaLinki | Q13882 |
SIGNORi | Q13882 |
Names & Taxonomyi
Protein namesi | Recommended name: Protein-tyrosine kinase 6 (EC:2.7.10.23 Publications)Alternative name(s): Breast tumor kinase Tyrosine-protein kinase BRK |
Gene namesi | Name:PTK6 Synonyms:BRK |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000101213.6 |
HGNCi | HGNC:9617, PTK6 |
MIMi | 602004, gene |
neXtProti | NX_Q13882 |
Subcellular locationi
Cytosol
- cytosol Source: HPA
Nucleus
- nuclear body Source: HPA
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
Plasma Membrane
- extrinsic component of cytoplasmic side of plasma membrane Source: GO_Central
- plasma membrane Source: HPA
Other locations
Keywords - Cellular componenti
Cell projection, Cytoplasm, Membrane, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 44 | W → A: Strong decrease in STAP2 phosphorylation. Markedly decreased interaction between SH3 domain the linker region. 2 Publications | 1 | |
Mutagenesisi | 66 | Y → A: Decrease in STAP2 phosphorylation. 1 Publication | 1 | |
Mutagenesisi | 105 | R → L: Decrease in STAP2 phosphorylation. 1 Publication | 1 | |
Mutagenesisi | 184 | W → A: Abrogates interaction between PTK6-domain kinase and PTK6-linker. Abrogates autophosphorylation and phosphorylation of KHDRBS1. 1 Publication | 1 | |
Mutagenesisi | 219 | K → M: Abolishes kinase activity and cell transformation, and phosphorylation of STAP2. 3 Publications | 1 | |
Mutagenesisi | 219 | K → R: Abolishes kinase activity. 1 Publication | 1 | |
Mutagenesisi | 342 | Y → A: 3-fold lower specific kinase activity. Decreased, but still significant, autophosphorylation. Decreased, but still significant, autophosphorylation; when associated with A-447. 1 Publication | 1 | |
Mutagenesisi | 447 | Y → F: Decrease in transforming potential and increase in the kinase activity level. Decreased, but still significant, autophosphorylation; when associated with A-342. 3 Publications | 1 |
Organism-specific databases
DisGeNETi | 5753 |
OpenTargetsi | ENSG00000101213 |
PharmGKBi | PA33960 |
Miscellaneous databases
Pharosi | Q13882, Tchem |
Chemistry databases
ChEMBLi | CHEMBL4601 |
DrugBanki | DB12010, Fostamatinib DB05294, Vandetanib DB15035, Zanubrutinib |
DrugCentrali | Q13882 |
GuidetoPHARMACOLOGYi | 2182 |
Polymorphism and mutation databases
BioMutai | PTK6 |
DMDMi | 8928302 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000088133 | 1 – 451 | Protein-tyrosine kinase 6Add BLAST | 451 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 13 | Phosphotyrosine; by autocatalysis1 Publication | 1 | |
Modified residuei | 61 | Phosphotyrosine; by autocatalysis1 Publication | 1 | |
Modified residuei | 66 | Phosphotyrosine; by autocatalysis1 Publication | 1 | |
Modified residuei | 114 | Phosphotyrosine; by autocatalysisCombined sources1 Publication | 1 | |
Modified residuei | 342 | Phosphotyrosine; by autocatalysis1 Publication | 1 | |
Modified residuei | 351 | Phosphotyrosine; by autocatalysis1 Publication | 1 | |
Modified residuei | 447 | Phosphotyrosine1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q13882 |
jPOSTi | Q13882 |
MassIVEi | Q13882 |
MaxQBi | Q13882 |
PaxDbi | Q13882 |
PeptideAtlasi | Q13882 |
PRIDEi | Q13882 |
ProteomicsDBi | 59709 [Q13882-1] 59710 [Q13882-2] |
PTM databases
iPTMneti | Q13882 |
PhosphoSitePlusi | Q13882 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000101213, Expressed in lower esophagus mucosa and 146 other tissues |
Genevisiblei | Q13882, HS |
Organism-specific databases
HPAi | ENSG00000101213, Tissue enhanced (esophagus, intestine, skin) |
Interactioni
Subunit structurei
Interacts with GAP-A.p65 (By similarity).
Interacts (via SH3 and SH2 domains) with KHDRBS1.
Interacts (via SH3 and SH2 domains) with phosphorylated IRS4.
Interacts with ADAM15.
Interacts (via SH3 domain) with SFPQ.
Interacts with EGFR and ERBB2.
Interacts with STAP2.
Interacts with PNX.
Interacts with SFPQ.
Interacts with PTK/ATK.
Interacts with CTNNB1.
By similarity10 PublicationsBinary interactionsi
Hide detailsQ13882
GO - Molecular functioni
- identical protein binding Source: IntAct
- signaling receptor binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 111720, 50 interactors |
DIPi | DIP-39785N |
IntActi | Q13882, 73 interactors |
MINTi | Q13882 |
STRINGi | 9606.ENSP00000442460 |
Chemistry databases
BindingDBi | Q13882 |
Miscellaneous databases
RNActi | Q13882, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | Q13882 |
SMRi | Q13882 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q13882 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 8 – 72 | SH3PROSITE-ProRule annotationAdd BLAST | 65 | |
Domaini | 78 – 170 | SH2PROSITE-ProRule annotationAdd BLAST | 93 | |
Domaini | 191 – 445 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 255 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 171 – 190 | LinkerAdd BLAST | 20 |
Domaini
Sequence similaritiesi
Keywords - Domaini
SH2 domain, SH3 domainPhylogenomic databases
eggNOGi | KOG0197, Eukaryota |
GeneTreei | ENSGT00940000161218 |
HOGENOMi | CLU_000288_7_2_1 |
InParanoidi | Q13882 |
OMAi | EDIYLSH |
OrthoDBi | 539311at2759 |
PhylomeDBi | Q13882 |
TreeFami | TF351634 |
Family and domain databases
CDDi | cd10358, SH2_PTK6_Brk, 1 hit |
Gene3Di | 3.30.505.10, 1 hit |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR035846, PTK6_SH2 IPR001245, Ser-Thr/Tyr_kinase_cat_dom IPR000980, SH2 IPR036860, SH2_dom_sf IPR036028, SH3-like_dom_sf IPR001452, SH3_domain IPR008266, Tyr_kinase_AS IPR020635, Tyr_kinase_cat_dom |
Pfami | View protein in Pfam PF07714, PK_Tyr_Ser-Thr, 1 hit PF00017, SH2, 1 hit PF00018, SH3_1, 1 hit |
PRINTSi | PR00401, SH2DOMAIN PR00452, SH3DOMAIN PR00109, TYRKINASE |
SMARTi | View protein in SMART SM00252, SH2, 1 hit SM00326, SH3, 1 hit SM00219, TyrKc, 1 hit |
SUPFAMi | SSF50044, SSF50044, 1 hit SSF55550, SSF55550, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00109, PROTEIN_KINASE_TYR, 1 hit PS50001, SH2, 1 hit PS50002, SH3, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MVSRDQAHLG PKYVGLWDFK SRTDEELSFR AGDVFHVARK EEQWWWATLL
60 70 80 90 100
DEAGGAVAQG YVPHNYLAER ETVESEPWFF GCISRSEAVR RLQAEGNATG
110 120 130 140 150
AFLIRVSEKP SADYVLSVRD TQAVRHYKIW RRAGGRLHLN EAVSFLSLPE
160 170 180 190 200
LVNYHRAQSL SHGLRLAAPC RKHEPEPLPH WDDWERPREE FTLCRKLGSG
210 220 230 240 250
YFGEVFEGLW KDRVQVAIKV ISRDNLLHQQ MLQSEIQAMK KLRHKHILAL
260 270 280 290 300
YAVVSVGDPV YIITELMAKG SLLELLRDSD EKVLPVSELL DIAWQVAEGM
310 320 330 340 350
CYLESQNYIH RDLAARNILV GENTLCKVGD FGLARLIKED VYLSHDHNIP
360 370 380 390 400
YKWTAPEALS RGHYSTKSDV WSFGILLHEM FSRGQVPYPG MSNHEAFLRV
410 420 430 440 450
DAGYRMPCPL ECPPSVHKLM LTCWCRDPEQ RPCFKALRER LSSFTSYENP
T
Sequence cautioni
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_041760 | 16 | L → F in a renal papillary sample; somatic mutation. 1 Publication | 1 | |
Natural variantiVAR_041761 | 436 | A → T1 PublicationCorresponds to variant dbSNP:rs56145017Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_042066 | 78 – 134 | WFFGC…WRRAG → AGHAGCAALQDLAACRGPAA PERGGVLPQPARACELPQGP EPVPRPAAGRALPEARA in isoform 2. 2 PublicationsAdd BLAST | 57 | |
Alternative sequenceiVSP_042067 | 135 – 451 | Missing in isoform 2. 2 PublicationsAdd BLAST | 317 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X78549 mRNA Translation: CAA55295.1 U61412 , U61406, U61407, U61408, U61409, U61410, U61411 Genomic DNA Translation: AAC34935.1 AK315232 mRNA Translation: BAG37660.1 AK301364 mRNA Translation: BAG62908.1 Sequence problems. AL121829 Genomic DNA No translation available. BC035843 mRNA Translation: AAH35843.1 |
CCDSi | CCDS13524.1 [Q13882-1] CCDS74750.1 [Q13882-2] |
PIRi | S49016 |
RefSeqi | NP_001243287.1, NM_001256358.1 [Q13882-2] NP_005966.1, NM_005975.3 [Q13882-1] |
Genome annotation databases
Ensembli | ENST00000217185; ENSP00000217185; ENSG00000101213 [Q13882-2] ENST00000542869; ENSP00000442460; ENSG00000101213 [Q13882-1] |
GeneIDi | 5753 |
KEGGi | hsa:5753 |
UCSCi | uc002yfg.5, human [Q13882-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X78549 mRNA Translation: CAA55295.1 U61412 , U61406, U61407, U61408, U61409, U61410, U61411 Genomic DNA Translation: AAC34935.1 AK315232 mRNA Translation: BAG37660.1 AK301364 mRNA Translation: BAG62908.1 Sequence problems. AL121829 Genomic DNA No translation available. BC035843 mRNA Translation: AAH35843.1 |
CCDSi | CCDS13524.1 [Q13882-1] CCDS74750.1 [Q13882-2] |
PIRi | S49016 |
RefSeqi | NP_001243287.1, NM_001256358.1 [Q13882-2] NP_005966.1, NM_005975.3 [Q13882-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1RJA | NMR | - | A | 75-174 | [»] | |
2KGT | NMR | - | A | 1-72 | [»] | |
5D7V | X-ray | 2.33 | A/B/C/D | 185-446 | [»] | |
5DA3 | X-ray | 1.70 | A | 185-446 | [»] | |
5H2U | X-ray | 2.24 | A/B/C/D | 185-446 | [»] | |
6CZ2 | X-ray | 2.50 | A | 182-443 | [»] | |
6CZ3 | X-ray | 1.80 | A | 182-443 | [»] | |
6CZ4 | X-ray | 1.50 | A | 182-443 | [»] | |
BMRBi | Q13882 | |||||
SMRi | Q13882 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 111720, 50 interactors |
DIPi | DIP-39785N |
IntActi | Q13882, 73 interactors |
MINTi | Q13882 |
STRINGi | 9606.ENSP00000442460 |
Chemistry databases
BindingDBi | Q13882 |
ChEMBLi | CHEMBL4601 |
DrugBanki | DB12010, Fostamatinib DB05294, Vandetanib DB15035, Zanubrutinib |
DrugCentrali | Q13882 |
GuidetoPHARMACOLOGYi | 2182 |
PTM databases
iPTMneti | Q13882 |
PhosphoSitePlusi | Q13882 |
Polymorphism and mutation databases
BioMutai | PTK6 |
DMDMi | 8928302 |
Proteomic databases
EPDi | Q13882 |
jPOSTi | Q13882 |
MassIVEi | Q13882 |
MaxQBi | Q13882 |
PaxDbi | Q13882 |
PeptideAtlasi | Q13882 |
PRIDEi | Q13882 |
ProteomicsDBi | 59709 [Q13882-1] 59710 [Q13882-2] |
Protocols and materials databases
Antibodypediai | 29774, 511 antibodies |
DNASUi | 5753 |
Genome annotation databases
Ensembli | ENST00000217185; ENSP00000217185; ENSG00000101213 [Q13882-2] ENST00000542869; ENSP00000442460; ENSG00000101213 [Q13882-1] |
GeneIDi | 5753 |
KEGGi | hsa:5753 |
UCSCi | uc002yfg.5, human [Q13882-1] |
Organism-specific databases
CTDi | 5753 |
DisGeNETi | 5753 |
EuPathDBi | HostDB:ENSG00000101213.6 |
GeneCardsi | PTK6 |
HGNCi | HGNC:9617, PTK6 |
HPAi | ENSG00000101213, Tissue enhanced (esophagus, intestine, skin) |
MIMi | 602004, gene |
neXtProti | NX_Q13882 |
OpenTargetsi | ENSG00000101213 |
PharmGKBi | PA33960 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0197, Eukaryota |
GeneTreei | ENSGT00940000161218 |
HOGENOMi | CLU_000288_7_2_1 |
InParanoidi | Q13882 |
OMAi | EDIYLSH |
OrthoDBi | 539311at2759 |
PhylomeDBi | Q13882 |
TreeFami | TF351634 |
Enzyme and pathway databases
BRENDAi | 2.7.10.2, 2681 |
PathwayCommonsi | Q13882 |
Reactomei | R-HSA-187577, SCF(Skp2)-mediated degradation of p27/p21 R-HSA-69231, Cyclin D associated events in G1 R-HSA-8847993, ERBB2 Activates PTK6 Signaling R-HSA-8849468, PTK6 Regulates Proteins Involved in RNA Processing R-HSA-8849469, PTK6 Regulates RTKs and Their Effectors AKT1 and DOK1 R-HSA-8849470, PTK6 Regulates Cell Cycle R-HSA-8849471, PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases R-HSA-8849472, PTK6 Down-Regulation R-HSA-8849473, PTK6 Expression R-HSA-8849474, PTK6 Activates STAT3 R-HSA-8857538, PTK6 promotes HIF1A stabilization |
SignaLinki | Q13882 |
SIGNORi | Q13882 |
Miscellaneous databases
BioGRID-ORCSi | 5753, 7 hits in 873 CRISPR screens |
EvolutionaryTracei | Q13882 |
GeneWikii | PTK6 |
GenomeRNAii | 5753 |
Pharosi | Q13882, Tchem |
PROi | PR:Q13882 |
RNActi | Q13882, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000101213, Expressed in lower esophagus mucosa and 146 other tissues |
Genevisiblei | Q13882, HS |
Family and domain databases
CDDi | cd10358, SH2_PTK6_Brk, 1 hit |
Gene3Di | 3.30.505.10, 1 hit |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR035846, PTK6_SH2 IPR001245, Ser-Thr/Tyr_kinase_cat_dom IPR000980, SH2 IPR036860, SH2_dom_sf IPR036028, SH3-like_dom_sf IPR001452, SH3_domain IPR008266, Tyr_kinase_AS IPR020635, Tyr_kinase_cat_dom |
Pfami | View protein in Pfam PF07714, PK_Tyr_Ser-Thr, 1 hit PF00017, SH2, 1 hit PF00018, SH3_1, 1 hit |
PRINTSi | PR00401, SH2DOMAIN PR00452, SH3DOMAIN PR00109, TYRKINASE |
SMARTi | View protein in SMART SM00252, SH2, 1 hit SM00326, SH3, 1 hit SM00219, TyrKc, 1 hit |
SUPFAMi | SSF50044, SSF50044, 1 hit SSF55550, SSF55550, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00109, PROTEIN_KINASE_TYR, 1 hit PS50001, SH2, 1 hit PS50002, SH3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PTK6_HUMAN | |
Accessioni | Q13882Primary (citable) accession number: Q13882 Secondary accession number(s): B2RCR3, B4DW46, Q58F01 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | November 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 210 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 20
Human chromosome 20: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations - Human and mouse protein kinases
Human and mouse protein kinases: classification and index