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Protein

Bleomycin hydrolase

Gene

BLMH

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B-aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity.By similarity

Catalytic activityi

Inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by hydrolysis of a carboxyamide bond of beta-aminoalanine, but also shows general aminopeptidase activity. The specificity varies somewhat with source, but amino acid arylamides of Met, Leu and Ala are preferred.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei731
Active sitei3721
Active sitei3961

GO - Molecular functioni

  • aminopeptidase activity Source: Reactome
  • carboxypeptidase activity Source: ProtInc
  • cysteine-type endopeptidase activity Source: ProtInc
  • cysteine-type peptidase activity Source: GO_Central
  • identical protein binding Source: IntAct

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease

Enzyme and pathway databases

BRENDAi3.4.22.40 2681
ReactomeiR-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
SABIO-RKiQ13867

Protein family/group databases

MEROPSiC01.084

Names & Taxonomyi

Protein namesi
Recommended name:
Bleomycin hydrolase (EC:3.4.22.40)
Short name:
BH
Short name:
BLM hydrolase
Short name:
BMH
Gene namesi
Name:BLMH
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000108578.14
HGNCiHGNC:1059 BLMH
MIMi602403 gene
neXtProtiNX_Q13867

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi642
MalaCardsiBLMH
OpenTargetsiENSG00000108578
Orphaneti364195 Resistance to bleomycine in the treatment of testicular cancer
PharmGKBiPA25370

Chemistry databases

DrugBankiDB00290 Bleomycin

Polymorphism and mutation databases

BioMutaiBLMH

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000505501 – 455Bleomycin hydrolaseAdd BLAST455

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei391N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ13867
MaxQBiQ13867
PaxDbiQ13867
PeptideAtlasiQ13867
PRIDEiQ13867
ProteomicsDBi59703

PTM databases

iPTMnetiQ13867
PhosphoSitePlusiQ13867

Expressioni

Gene expression databases

BgeeiENSG00000108578 Expressed in 208 organ(s), highest expression level in upper arm skin
CleanExiHS_BLMH
ExpressionAtlasiQ13867 baseline and differential
GenevisibleiQ13867 HS

Organism-specific databases

HPAiHPA039548
HPA064307

Interactioni

Subunit structurei

Homohexamer.

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi107111, 64 interactors
IntActiQ13867, 45 interactors
MINTiQ13867
STRINGi9606.ENSP00000261714

Structurei

Secondary structure

1455
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ13867
SMRiQ13867
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ13867

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C1 family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4128 Eukaryota
COG3579 LUCA
GeneTreeiENSGT00390000001735
HOGENOMiHOG000064089
HOVERGENiHBG002388
InParanoidiQ13867
KOiK01372
OMAiGGQWDMI
OrthoDBiEOG091G07WT
PhylomeDBiQ13867
TreeFamiTF323372

Family and domain databases

CDDicd00585 Peptidase_C1B, 1 hit
InterProiView protein in InterPro
IPR038765 Papain_like_cys_pep_sf
IPR000169 Pept_cys_AS
IPR004134 Peptidase_C1B
PANTHERiPTHR10363 PTHR10363, 1 hit
PfamiView protein in Pfam
PF03051 Peptidase_C1_2, 1 hit
PIRSFiPIRSF005700 PepC, 1 hit
SUPFAMiSSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS00139 THIOL_PROTEASE_CYS, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 6 potential isoforms that are computationally mapped.Show allAlign All

Q13867-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSSSGLNSEK VAALIQKLNS DPQFVLAQNV GTTHDLLDIC LKRATVQRAQ
60 70 80 90 100
HVFQHAVPQE GKPITNQKSS GRCWIFSCLN VMRLPFMKKL NIEEFEFSQS
110 120 130 140 150
YLFFWDKVER CYFFLSAFVD TAQRKEPEDG RLVQFLLMNP ANDGGQWDML
160 170 180 190 200
VNIVEKYGVI PKKCFPESYT TEATRRMNDI LNHKMREFCI RLRNLVHSGA
210 220 230 240 250
TKGEISATQD VMMEEIFRVV CICLGNPPET FTWEYRDKDK NYQKIGPITP
260 270 280 290 300
LEFYREHVKP LFNMEDKICL VNDPRPQHKY NKLYTVEYLS NMVGGRKTLY
310 320 330 340 350
NNQPIDFLKK MVAASIKDGE AVWFGCDVGK HFNSKLGLSD MNLYDHELVF
360 370 380 390 400
GVSLKNMNKA ERLTFGESLM THAMTFTAVS EKDDQDGAFT KWRVENSWGE
410 420 430 440 450
DHGHKGYLCM TDEWFSEYVY EVVVDRKHVP EEVLAVLEQE PIILPAWDPM

GALAE
Length:455
Mass (Da):52,562
Last modified:November 1, 1996 - v1
Checksum:i577E86241EB0D460
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
K7ES02K7ES02_HUMAN
Bleomycin hydrolase
BLMH
318Annotation score:
K7ESE8K7ESE8_HUMAN
Bleomycin hydrolase
BLMH
231Annotation score:
J3KSD8J3KSD8_HUMAN
Bleomycin hydrolase
BLMH
262Annotation score:
J3KS79J3KS79_HUMAN
Bleomycin hydrolase
BLMH
71Annotation score:
K7ENH5K7ENH5_HUMAN
Bleomycin hydrolase
BLMH
93Annotation score:
K7EMJ3K7EMJ3_HUMAN
Bleomycin hydrolase
BLMH
67Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_010896443I → V Common polymorphism. 2 PublicationsCorresponds to variant dbSNP:rs1050565EnsemblClinVar.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92106 mRNA Translation: CAA63078.1
BT007018 mRNA Translation: AAP35664.1
AK223403 mRNA Translation: BAD97123.1
AK312896 mRNA Translation: BAG35743.1
CH471159 Genomic DNA Translation: EAW51224.1
BC003616 mRNA Translation: AAH03616.1
AF091082 mRNA Translation: AAC72951.1
CCDSiCCDS32604.1
RefSeqiNP_000377.1, NM_000386.3
UniGeneiHs.371914

Genome annotation databases

EnsembliENST00000261714; ENSP00000261714; ENSG00000108578
GeneIDi642
KEGGihsa:642
UCSCiuc002hez.3 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92106 mRNA Translation: CAA63078.1
BT007018 mRNA Translation: AAP35664.1
AK223403 mRNA Translation: BAD97123.1
AK312896 mRNA Translation: BAG35743.1
CH471159 Genomic DNA Translation: EAW51224.1
BC003616 mRNA Translation: AAH03616.1
AF091082 mRNA Translation: AAC72951.1
CCDSiCCDS32604.1
RefSeqiNP_000377.1, NM_000386.3
UniGeneiHs.371914

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CB5X-ray2.59A/B/C2-454[»]
2CB5X-ray1.85A/B2-454[»]
ProteinModelPortaliQ13867
SMRiQ13867
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107111, 64 interactors
IntActiQ13867, 45 interactors
MINTiQ13867
STRINGi9606.ENSP00000261714

Chemistry databases

DrugBankiDB00290 Bleomycin

Protein family/group databases

MEROPSiC01.084

PTM databases

iPTMnetiQ13867
PhosphoSitePlusiQ13867

Polymorphism and mutation databases

BioMutaiBLMH

Proteomic databases

EPDiQ13867
MaxQBiQ13867
PaxDbiQ13867
PeptideAtlasiQ13867
PRIDEiQ13867
ProteomicsDBi59703

Protocols and materials databases

DNASUi642
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000261714; ENSP00000261714; ENSG00000108578
GeneIDi642
KEGGihsa:642
UCSCiuc002hez.3 human

Organism-specific databases

CTDi642
DisGeNETi642
EuPathDBiHostDB:ENSG00000108578.14
GeneCardsiBLMH
HGNCiHGNC:1059 BLMH
HPAiHPA039548
HPA064307
MalaCardsiBLMH
MIMi602403 gene
neXtProtiNX_Q13867
OpenTargetsiENSG00000108578
Orphaneti364195 Resistance to bleomycine in the treatment of testicular cancer
PharmGKBiPA25370
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4128 Eukaryota
COG3579 LUCA
GeneTreeiENSGT00390000001735
HOGENOMiHOG000064089
HOVERGENiHBG002388
InParanoidiQ13867
KOiK01372
OMAiGGQWDMI
OrthoDBiEOG091G07WT
PhylomeDBiQ13867
TreeFamiTF323372

Enzyme and pathway databases

BRENDAi3.4.22.40 2681
ReactomeiR-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
SABIO-RKiQ13867

Miscellaneous databases

ChiTaRSiBLMH human
EvolutionaryTraceiQ13867
GeneWikiiBleomycin_hydrolase
BLMH
GenomeRNAii642
PROiPR:Q13867
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000108578 Expressed in 208 organ(s), highest expression level in upper arm skin
CleanExiHS_BLMH
ExpressionAtlasiQ13867 baseline and differential
GenevisibleiQ13867 HS

Family and domain databases

CDDicd00585 Peptidase_C1B, 1 hit
InterProiView protein in InterPro
IPR038765 Papain_like_cys_pep_sf
IPR000169 Pept_cys_AS
IPR004134 Peptidase_C1B
PANTHERiPTHR10363 PTHR10363, 1 hit
PfamiView protein in Pfam
PF03051 Peptidase_C1_2, 1 hit
PIRSFiPIRSF005700 PepC, 1 hit
SUPFAMiSSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS00139 THIOL_PROTEASE_CYS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiBLMH_HUMAN
AccessioniPrimary (citable) accession number: Q13867
Secondary accession number(s): B2R796, Q53F86, Q9UER9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: November 7, 2018
This is version 178 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  7. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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