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Protein

Nucleolar GTP-binding protein 2

Gene

GNL2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation (By similarity). May promote cell proliferation possibly by increasing p53/TP53 protein levels, and consequently those of its downstream product CDKN1A/p21, and decreasing RPL23A protein levels (PubMed:26203195).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi317 – 324GTPSequence analysis8
Nucleotide bindingi361 – 365GTPSequence analysis5

GO - Molecular functioni

  • GTPase activity Source: UniProtKB
  • GTP binding Source: UniProtKB
  • RNA binding Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processRibosome biogenesis
LigandGTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleolar GTP-binding protein 2
Alternative name(s):
Autoantigen NGP-1
Gene namesi
Name:GNL2
Synonyms:NGP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000134697.12
HGNCiHGNC:29925 GNL2
MIMi609365 gene
neXtProtiNX_Q13823

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi33 – 35RDR → ADA: Diffused nuclear localization, loss of nucleolar localization. 1 Publication3
Mutagenesisi39 – 40RR → AA: Diffused nuclear localization, loss of nucleolar localization. 1 Publication2
Mutagenesisi39 – 40RR → AA: No effect on nucleolar localization. 1 Publication2
Mutagenesisi49 – 50RR → AA: Diffused nuclear localization, loss of nucleolar localization. 1 Publication2
Mutagenesisi227 – 229RDP → AAA: Loss of GTP-binding. Efficient localization to the nucleus, but excluded from nucleoli. 1 Publication3
Mutagenesisi258D → A: Loss of GTP-binding. Punctate pattern throughout the nucleus, including nucleoli. 1 Publication1
Mutagenesisi323K → A: No effect on GTP-binding, nor on nucleolar localization. 1 Publication1
Mutagenesisi324S → A: No effect on GTP-binding, nor on nucleolar localization. 1 Publication1
Mutagenesisi325S → A: No effect on GTP-binding, nor on nucleolar localization. 1 Publication1
Mutagenesisi343 – 345GET → AEA: No effect on GTP-binding, nor on nucleolar localization. 1 Publication3
Mutagenesisi363 – 364PG → AA: No effect on GTP-binding, nor on nucleolar localization. 1 Publication2
Mutagenesisi463P → A: No effect on nucleolar localization; when associated with A-475. 1 Publication1
Mutagenesisi475P → A: No effect on nucleolar localization; when associated with A-463. 1 Publication1
Mutagenesisi682 – 684RRR → AAA: No effect on nucleolar localization. Predominantly cytoplasmic, with some nucleolar staining; when associated with 711-AAA-713. Diffused localization to both cytoplasm and nucleus; when associated with 711-AAA-713 and 724-AAA-726. 1 Publication3
Mutagenesisi711 – 713KKK → AAA: No effect on nucleolar localization Predominantly cytoplasmic, with some nucleolar staining; when associated with 682-AAA-684. Strong nucleolar localization, with some cytoplasmic staining; when associated with 724-AAA-726. Diffused localization to both cytoplasm and nucleus; when associated with 682-AAA-684 and 724-AAA-726. 1 Publication3
Mutagenesisi724 – 726RKK → AAA: No effect on nucleolar localization. Strong nucleolar localization, with some cytoplasmic staining; when associated with 711-AAA-713. Diffused localization to both cytoplasm and nucleus; when associated with 682-AAA-684 and 711-AAA-713. 1 Publication3

Organism-specific databases

DisGeNETi29889
OpenTargetsiENSG00000134697
PharmGKBiPA134944852

Polymorphism and mutation databases

BioMutaiGNL2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002158061 – 731Nucleolar GTP-binding protein 2Add BLAST731

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei504PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ13823
PaxDbiQ13823
PeptideAtlasiQ13823
PRIDEiQ13823
ProteomicsDBi59695

2D gel databases

SWISS-2DPAGEiQ13823

PTM databases

iPTMnetiQ13823
PhosphoSitePlusiQ13823

Expressioni

Tissue specificityi

Widely expressed, with the highest expression level in testis.1 Publication

Developmental stagei

Up-regulated in actively dividing cells, including in peripheral blood mononuclear cells stimulated with Concanavalin-A. Down-regulated in differentiating cells, including in neural precursor cells induced to differentiate into astrocytes.1 Publication

Gene expression databases

BgeeiENSG00000134697 Expressed in 228 organ(s), highest expression level in testis
CleanExiHS_GNL2
ExpressionAtlasiQ13823 baseline and differential
GenevisibleiQ13823 HS

Organism-specific databases

HPAiHPA027163
HPA060026

Interactioni

Subunit structurei

Interacts with LYAR and RPL23A (PubMed:26203195). Interacts with the nuclear importin-beta receptor and, at a lower extent, with importin-alpha (PubMed:21495629).2 Publications

Protein-protein interaction databases

BioGridi118942, 91 interactors
IntActiQ13823, 25 interactors
STRINGi9606.ENSP00000362153

Structurei

3D structure databases

ProteinModelPortaliQ13823
SMRiQ13823
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini207 – 368CP-type GPROSITE-ProRule annotationAdd BLAST162

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi565 – 571Poly-Glu7

Sequence similaritiesi

Belongs to the TRAFAC class YlqF/YawG GTPase family. NOG2 subfamily.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2423 Eukaryota
COG1161 LUCA
GeneTreeiENSGT00810000125524
HOGENOMiHOG000189714
HOVERGENiHBG052643
InParanoidiQ13823
KOiK14537
OMAiFAKSEEQ
OrthoDBiEOG091G055D
PhylomeDBiQ13823
TreeFamiTF105668

Family and domain databases

Gene3Di1.10.1580.10, 1 hit
InterProiView protein in InterPro
IPR030378 G_CP_dom
IPR023179 GTP-bd_ortho_bundle_sf
IPR006073 GTP_binding_domain
IPR024929 NOG2
IPR012971 NOG2_N_dom
IPR027417 P-loop_NTPase
PANTHERiPTHR11089:SF9 PTHR11089:SF9, 1 hit
PfamiView protein in Pfam
PF01926 MMR_HSR1, 1 hit
PF08153 NGP1NT, 1 hit
PRINTSiPR00326 GTP1OBG
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS51721 G_CP, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q13823-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVKPKYKGRS TINPSKASTN PDRVQGAGGQ NMRDRATIRR LNMYRQKERR
60 70 80 90 100
NSRGKIIKPL QYQSTVASGT VARVEPNIKW FGNTRVIKQS SLQKFQEEMD
110 120 130 140 150
TVMKDPYKVV MKQSKLPMSL LHDRIRPHNL KVHILDTESF ETTFGPKSQR
160 170 180 190 200
KRPNLFASDM QSLIENAEMS TESYDQGKDR DLVTEDTGVR NEAQEEIYKK
210 220 230 240 250
GQSKRIWGEL YKVIDSSDVV VQVLDARDPM GTRSPHIETY LKKEKPWKHL
260 270 280 290 300
IFVLNKCDLV PTWATKRWVA VLSQDYPTLA FHASLTNPFG KGAFIQLLRQ
310 320 330 340 350
FGKLHTDKKQ ISVGFIGYPN VGKSSVINTL RSKKVCNVAP IAGETKVWQY
360 370 380 390 400
ITLMRRIFLI DCPGVVYPSE DSETDIVLKG VVQVEKIKSP EDHIGAVLER
410 420 430 440 450
AKPEYISKTY KIDSWENAED FLEKLAFRTG KLLKGGEPDL QTVGKMVLND
460 470 480 490 500
WQRGRIPFFV KPPNAEPLVA PQLLPSSSLE VVPEAAQNNP GEEVTETAGE
510 520 530 540 550
GSESIIKEET EENSHCDANT EMQQILTRVR QNFGKINVVP QFSGDDLVPV
560 570 580 590 600
EVSDLEEELE SFSDEEEEEQ EQQRDDAEES SSEPEEENVG NDTKAVIKAL
610 620 630 640 650
DEKIAKYQKF LDKAKAKKFS AVRISKGLSE KIFAKPEEQR KTLEEDVDDR
660 670 680 690 700
APSKKGKKRK AQREEEQEHS NKAPRALTSK ERRRAVRQQR PKKVGVRYYE
710 720 730
THNVKNRNRN KKKTNDSEGQ KHKRKKFRQK Q
Length:731
Mass (Da):83,655
Last modified:November 1, 1996 - v1
Checksum:iE90C638B58C0886E
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H0YG10H0YG10_HUMAN
Nucleolar GTP-binding protein 2
GNL2
233Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti284S → I in AAH00107 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_050291452Q → H. Corresponds to variant dbSNP:rs12025870Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L05425 mRNA Translation: AAC37588.1
BC000107 mRNA Translation: AAH00107.1
BC009250 mRNA Translation: AAH09250.1
CCDSiCCDS421.1
RefSeqiNP_037417.1, NM_013285.2
UniGeneiHs.75528

Genome annotation databases

EnsembliENST00000373062; ENSP00000362153; ENSG00000134697
GeneIDi29889
KEGGihsa:29889

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L05425 mRNA Translation: AAC37588.1
BC000107 mRNA Translation: AAH00107.1
BC009250 mRNA Translation: AAH09250.1
CCDSiCCDS421.1
RefSeqiNP_037417.1, NM_013285.2
UniGeneiHs.75528

3D structure databases

ProteinModelPortaliQ13823
SMRiQ13823
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi118942, 91 interactors
IntActiQ13823, 25 interactors
STRINGi9606.ENSP00000362153

PTM databases

iPTMnetiQ13823
PhosphoSitePlusiQ13823

Polymorphism and mutation databases

BioMutaiGNL2

2D gel databases

SWISS-2DPAGEiQ13823

Proteomic databases

EPDiQ13823
PaxDbiQ13823
PeptideAtlasiQ13823
PRIDEiQ13823
ProteomicsDBi59695

Protocols and materials databases

DNASUi29889
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000373062; ENSP00000362153; ENSG00000134697
GeneIDi29889
KEGGihsa:29889

Organism-specific databases

CTDi29889
DisGeNETi29889
EuPathDBiHostDB:ENSG00000134697.12
GeneCardsiGNL2
HGNCiHGNC:29925 GNL2
HPAiHPA027163
HPA060026
MIMi609365 gene
neXtProtiNX_Q13823
OpenTargetsiENSG00000134697
PharmGKBiPA134944852
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2423 Eukaryota
COG1161 LUCA
GeneTreeiENSGT00810000125524
HOGENOMiHOG000189714
HOVERGENiHBG052643
InParanoidiQ13823
KOiK14537
OMAiFAKSEEQ
OrthoDBiEOG091G055D
PhylomeDBiQ13823
TreeFamiTF105668

Miscellaneous databases

ChiTaRSiGNL2 human
GeneWikiiGNL2
GenomeRNAii29889
PROiPR:Q13823
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000134697 Expressed in 228 organ(s), highest expression level in testis
CleanExiHS_GNL2
ExpressionAtlasiQ13823 baseline and differential
GenevisibleiQ13823 HS

Family and domain databases

Gene3Di1.10.1580.10, 1 hit
InterProiView protein in InterPro
IPR030378 G_CP_dom
IPR023179 GTP-bd_ortho_bundle_sf
IPR006073 GTP_binding_domain
IPR024929 NOG2
IPR012971 NOG2_N_dom
IPR027417 P-loop_NTPase
PANTHERiPTHR11089:SF9 PTHR11089:SF9, 1 hit
PfamiView protein in Pfam
PF01926 MMR_HSR1, 1 hit
PF08153 NGP1NT, 1 hit
PRINTSiPR00326 GTP1OBG
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS51721 G_CP, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNOG2_HUMAN
AccessioniPrimary (citable) accession number: Q13823
Secondary accession number(s): Q9BWN7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: November 7, 2018
This is version 173 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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