UniProtKB - Q13765 (NACA_HUMAN)
Nascent polypeptide-associated complex subunit alpha
NACA
Functioni
Prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. Also reduces the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites). May act as a specific coactivator for JUN, binding to DNA and stabilizing the interaction of JUN homodimers with target gene promoters.
3 PublicationsGO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- transcription coactivator activity Source: BHF-UCL
GO - Biological processi
- cardiac ventricle development Source: BHF-UCL
- heart trabecula morphogenesis Source: BHF-UCL
- negative regulation of protein localization to endoplasmic reticulum Source: ComplexPortal
- negative regulation of striated muscle cell apoptotic process Source: BHF-UCL
- negative regulation of transcription from RNA polymerase II promoter involved in heart development Source: BHF-UCL
- positive regulation of cell proliferation involved in heart morphogenesis Source: BHF-UCL
- positive regulation of skeletal muscle tissue growth Source: BHF-UCL
- positive regulation of transcription from RNA polymerase II promoter involved in heart development Source: BHF-UCL
- protein transport Source: UniProtKB-KW
- regulation of skeletal muscle fiber development Source: BHF-UCL
- skeletal muscle tissue regeneration Source: BHF-UCL
- translation Source: ProtInc
- wound healing Source: BHF-UCL
Keywordsi
Molecular function | Chaperone, DNA-binding |
Biological process | Protein transport, Transcription, Transport |
Enzyme and pathway databases
PathwayCommonsi | Q13765 |
SignaLinki | Q13765 |
SIGNORi | Q13765 |
Names & Taxonomyi
Protein namesi | Recommended name: Nascent polypeptide-associated complex subunit alphaShort name: NAC-alpha Alternative name(s): Alpha-NAC Allergen: Hom s 2 |
Gene namesi | Name:NACA ORF Names:HSD48 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:7629, NACA |
MIMi | 601234, gene |
neXtProti | NX_Q13765 |
VEuPathDBi | HostDB:ENSG00000196531 |
Subcellular locationi
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Nucleus
- nucleus Source: BHF-UCL
Other locations
- cytoplasm Source: ComplexPortal
- nascent polypeptide-associated complex Source: ComplexPortal
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Allergenic propertiesi
Keywords - Diseasei
AllergenOrganism-specific databases
DisGeNETi | 4666 |
OpenTargetsi | ENSG00000196531 |
PharmGKBi | PA31433 |
Miscellaneous databases
Pharosi | Q13765, Tbio |
Protein family/group databases
Allergomei | 3323, Hom s 2.0101 412, Hom s 2 |
Genetic variation databases
BioMutai | NACA |
DMDMi | 71151996 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000135576 | 1 – 215 | Nascent polypeptide-associated complex subunit alphaAdd BLAST | 215 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 43 | Phosphoserine; by ILK11 Publication | 1 | |
Modified residuei | 132 | PhosphoserineCombined sources | 1 | |
Modified residuei | 142 | N6-acetyllysine; alternateCombined sources | 1 | |
Cross-linki | 142 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources | ||
Modified residuei | 159 | Phosphothreonine; by GSK3-betaBy similarity | 1 | |
Modified residuei | 161 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 166 | PhosphoserineCombined sources1 Publication | 1 | |
Modified residuei | 186 | PhosphoserineCombined sources | 1 | |
Modified residuei | 191 | PhosphoserineCombined sources | 1 | |
Modified residuei | 203 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q13765 |
jPOSTi | Q13765 |
MassIVEi | Q13765 |
MaxQBi | Q13765 |
PeptideAtlasi | Q13765 |
PRIDEi | Q13765 |
ProteomicsDBi | 28706 59679 [Q13765-1] |
TopDownProteomicsi | Q13765-1 [Q13765-1] |
PTM databases
iPTMneti | Q13765 |
MetOSitei | Q13765 |
SwissPalmi | Q13765 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000196531, Expressed in testis and 253 other tissues |
ExpressionAtlasi | Q13765, baseline and differential |
Genevisiblei | Q13765, HS |
Organism-specific databases
HPAi | ENSG00000196531, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with TBP and JUN (By similarity). Part of the nascent polypeptide-associated complex (NAC), which is a heterodimer of NACA and BTF3 (via NAC-A/B domains). NAC associates with ribosomes through the BTF3/NACB subunit and contacts the ribosomal protein L23, which is positioned near the exiting site. Both subunits can contact nascent polypeptide chains. NACA may also form homodimers, and only this form binds DNA.
By similarity4 PublicationsBinary interactionsi
Q13765
With | #Exp. | IntAct |
---|---|---|
APP [P05067] | 3 | EBI-712216,EBI-77613 |
TXLNA [P40222] | 2 | EBI-712216,EBI-359793 |
UNG - isoform 1 [P13051-2] | 3 | EBI-712216,EBI-25834258 |
Protein-protein interaction databases
BioGRIDi | 110748, 149 interactors |
ComplexPortali | CPX-676, Nascent polypeptide-associated complex |
DIPi | DIP-43878N |
IntActi | Q13765, 46 interactors |
MINTi | Q13765 |
Structurei
Secondary structure
3D structure databases
SMRi | Q13765 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q13765 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 70 – 135 | NAC-A/BPROSITE-ProRule annotationAdd BLAST | 66 | |
Domaini | 176 – 213 | UBAAdd BLAST | 38 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 81 | DisorderedSequence analysisAdd BLAST | 81 | |
Regioni | 69 – 80 | Required for DNA-bindingBy similarityAdd BLAST | 12 | |
Regioni | 93 – 108 | RNA/DNA-bindingAdd BLAST | 16 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 11 – 31 | Polar residuesSequence analysisAdd BLAST | 21 | |
Compositional biasi | 40 – 54 | Polar residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 61 – 80 | Basic and acidic residuesSequence analysisAdd BLAST | 20 |
Domaini
Sequence similaritiesi
Phylogenomic databases
GeneTreei | ENSGT00440000033468 |
HOGENOMi | CLU_057806_1_2_1 |
InParanoidi | Q13765 |
OrthoDBi | 1331328at2759 |
PhylomeDBi | Q13765 |
TreeFami | TF313348 |
Family and domain databases
Gene3Di | 2.20.70.30, 1 hit |
IDEALi | IID00472 |
InterProi | View protein in InterPro IPR016641, EGD2/NACA IPR044034, NAC-like_UBA IPR038187, NAC_A/B_dom_sf IPR002715, Nas_poly-pep-assoc_cplx_dom |
PANTHERi | PTHR21713, PTHR21713, 1 hit |
Pfami | View protein in Pfam PF19026, HYPK_UBA, 1 hit PF01849, NAC, 1 hit |
PIRSFi | PIRSF015901, NAC_alpha, 1 hit |
SMARTi | View protein in SMART SM01407, NAC, 1 hit |
PROSITEi | View protein in PROSITE PS51151, NAC_AB, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 9 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MPGEATETVP ATEQELPQPQ AETGSGTESD SDESVPELEE QDSTQATTQQ
60 70 80 90 100
AQLAAAAEID EEPVSKAKQS RSEKKARKAM SKLGLRQVTG VTRVTIRKSK
110 120 130 140 150
NILFVITKPD VYKSPASDTY IVFGEAKIED LSQQAQLAAA EKFKVQGEAV
160 170 180 190 200
SNIQENTQTP TVQEESEEEE VDETGVEVKD IELVMSQANV SRAKAVRALK
210
NNSNDIVNAI MELTM
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Computationally mapped potential isoform sequencesi
There are 9 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH0YHX9 | H0YHX9_HUMAN | Nascent polypeptide-associated comp... | NACA | 235 | Annotation score: | ||
F8VNW4 | F8VNW4_HUMAN | Nascent polypeptide-associated comp... | NACA | 148 | Annotation score: | ||
F8W0W4 | F8W0W4_HUMAN | Nascent polypeptide-associated comp... | NACA | 198 | Annotation score: | ||
F8VZ58 | F8VZ58_HUMAN | Nascent polypeptide-associated comp... | NACA | 126 | Annotation score: | ||
F8W029 | F8W029_HUMAN | Nascent polypeptide-associated comp... | NACA | 72 | Annotation score: | ||
A0A7I2V2K5 | A0A7I2V2K5_HUMAN | Nascent polypeptide-associated comp... | NACA | 211 | Annotation score: | ||
A0A7I2V473 | A0A7I2V473_HUMAN | Nascent polypeptide-associated comp... | NACA | 295 | Annotation score: | ||
F8VZJ2 | F8VZJ2_HUMAN | Nascent polypeptide-associated comp... | NACA | 136 | Annotation score: | ||
F8W0C7 | F8W0C7_HUMAN | Nascent polypeptide-associated comp... | NACA | 34 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 213 | L → S in BAD96805 (Ref. 7) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY034001 mRNA Translation: AAK57544.1 AY911673 mRNA Translation: AAX14393.1 X80909 mRNA Translation: CAA56869.1 AF054187 mRNA Translation: AAC99403.1 AK090650 mRNA Translation: BAG52208.1 AK311904 mRNA Translation: BAG34845.1 CR450295 mRNA Translation: CAG29291.1 AK223085 mRNA Translation: BAD96805.1 AC117378 Genomic DNA No translation available. CH471054 Genomic DNA Translation: EAW96960.1 BC105120 mRNA Translation: AAI05121.1 BC105122 mRNA Translation: AAI05123.1 BC106041 mRNA Translation: AAI06042.1 AY605660 mRNA Translation: AAV83778.1 Different initiation. |
CCDSi | CCDS31837.1 [Q13765-1] |
PIRi | S49326 |
RefSeqi | NP_001106672.1, NM_001113201.2 [Q13765-1] NP_001106673.1, NM_001113202.1 [Q13765-1] NP_001106674.2, NM_001113203.2 NP_001307122.1, NM_001320193.1 [Q13765-1] NP_001307123.1, NM_001320194.1 NP_005585.1, NM_005594.5 [Q13765-1] |
Genome annotation databases
Ensembli | ENST00000356769; ENSP00000349212; ENSG00000196531 ENST00000393891; ENSP00000377469; ENSG00000196531 ENST00000546392; ENSP00000446801; ENSG00000196531 ENST00000552540; ENSP00000447821; ENSG00000196531 ENST00000678066; ENSP00000503345; ENSG00000196531 |
GeneIDi | 4666 |
KEGGi | hsa:4666 |
UCSCi | uc001sly.3, human [Q13765-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY034001 mRNA Translation: AAK57544.1 AY911673 mRNA Translation: AAX14393.1 X80909 mRNA Translation: CAA56869.1 AF054187 mRNA Translation: AAC99403.1 AK090650 mRNA Translation: BAG52208.1 AK311904 mRNA Translation: BAG34845.1 CR450295 mRNA Translation: CAG29291.1 AK223085 mRNA Translation: BAD96805.1 AC117378 Genomic DNA No translation available. CH471054 Genomic DNA Translation: EAW96960.1 BC105120 mRNA Translation: AAI05121.1 BC105122 mRNA Translation: AAI05123.1 BC106041 mRNA Translation: AAI06042.1 AY605660 mRNA Translation: AAV83778.1 Different initiation. |
CCDSi | CCDS31837.1 [Q13765-1] |
PIRi | S49326 |
RefSeqi | NP_001106672.1, NM_001113201.2 [Q13765-1] NP_001106673.1, NM_001113202.1 [Q13765-1] NP_001106674.2, NM_001113203.2 NP_001307122.1, NM_001320193.1 [Q13765-1] NP_001307123.1, NM_001320194.1 NP_005585.1, NM_005594.5 [Q13765-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3LKX | X-ray | 2.50 | B | 84-136 | [»] | |
3MCB | X-ray | 1.90 | A | 79-132 | [»] | |
3MCE | X-ray | 2.40 | A/B/C/D | 81-133 | [»] | |
SMRi | Q13765 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 110748, 149 interactors |
ComplexPortali | CPX-676, Nascent polypeptide-associated complex |
DIPi | DIP-43878N |
IntActi | Q13765, 46 interactors |
MINTi | Q13765 |
Protein family/group databases
Allergomei | 3323, Hom s 2.0101 412, Hom s 2 |
PTM databases
iPTMneti | Q13765 |
MetOSitei | Q13765 |
SwissPalmi | Q13765 |
Genetic variation databases
BioMutai | NACA |
DMDMi | 71151996 |
Proteomic databases
EPDi | Q13765 |
jPOSTi | Q13765 |
MassIVEi | Q13765 |
MaxQBi | Q13765 |
PeptideAtlasi | Q13765 |
PRIDEi | Q13765 |
ProteomicsDBi | 28706 59679 [Q13765-1] |
TopDownProteomicsi | Q13765-1 [Q13765-1] |
Protocols and materials databases
Antibodypediai | 28384, 94 antibodies from 17 providers |
DNASUi | 4666 |
Genome annotation databases
Ensembli | ENST00000356769; ENSP00000349212; ENSG00000196531 ENST00000393891; ENSP00000377469; ENSG00000196531 ENST00000546392; ENSP00000446801; ENSG00000196531 ENST00000552540; ENSP00000447821; ENSG00000196531 ENST00000678066; ENSP00000503345; ENSG00000196531 |
GeneIDi | 4666 |
KEGGi | hsa:4666 |
UCSCi | uc001sly.3, human [Q13765-1] |
Organism-specific databases
CTDi | 4666 |
DisGeNETi | 4666 |
GeneCardsi | NACA |
HGNCi | HGNC:7629, NACA |
HPAi | ENSG00000196531, Low tissue specificity |
MIMi | 601234, gene |
neXtProti | NX_Q13765 |
OpenTargetsi | ENSG00000196531 |
PharmGKBi | PA31433 |
VEuPathDBi | HostDB:ENSG00000196531 |
GenAtlasi | Search... |
Phylogenomic databases
GeneTreei | ENSGT00440000033468 |
HOGENOMi | CLU_057806_1_2_1 |
InParanoidi | Q13765 |
OrthoDBi | 1331328at2759 |
PhylomeDBi | Q13765 |
TreeFami | TF313348 |
Enzyme and pathway databases
PathwayCommonsi | Q13765 |
SignaLinki | Q13765 |
SIGNORi | Q13765 |
Miscellaneous databases
BioGRID-ORCSi | 4666, 505 hits in 1050 CRISPR screens |
ChiTaRSi | NACA, human |
EvolutionaryTracei | Q13765 |
GeneWikii | NACA_(gene) |
GenomeRNAii | 4666 |
Pharosi | Q13765, Tbio |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000196531, Expressed in testis and 253 other tissues |
ExpressionAtlasi | Q13765, baseline and differential |
Genevisiblei | Q13765, HS |
Family and domain databases
Gene3Di | 2.20.70.30, 1 hit |
IDEALi | IID00472 |
InterProi | View protein in InterPro IPR016641, EGD2/NACA IPR044034, NAC-like_UBA IPR038187, NAC_A/B_dom_sf IPR002715, Nas_poly-pep-assoc_cplx_dom |
PANTHERi | PTHR21713, PTHR21713, 1 hit |
Pfami | View protein in Pfam PF19026, HYPK_UBA, 1 hit PF01849, NAC, 1 hit |
PIRSFi | PIRSF015901, NAC_alpha, 1 hit |
SMARTi | View protein in SMART SM01407, NAC, 1 hit |
PROSITEi | View protein in PROSITE PS51151, NAC_AB, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NACA_HUMAN | |
Accessioni | Q13765Primary (citable) accession number: Q13765 Secondary accession number(s): A8MTN7 Q53G46 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2005 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 189 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Allergens
Nomenclature of allergens and list of entries - Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families