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Protein

Nascent polypeptide-associated complex subunit alpha

Gene

NACA

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. Also reduces the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites). May act as a specific coactivator for JUN, binding to DNA and stabilizing the interaction of JUN homodimers with target gene promoters.3 Publications

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • transcription coactivator activity Source: BHF-UCL

GO - Biological processi

Keywordsi

Molecular functionChaperone, DNA-binding
Biological processProtein transport, Transcription, Transport

Enzyme and pathway databases

SIGNORiQ13765

Names & Taxonomyi

Protein namesi
Recommended name:
Nascent polypeptide-associated complex subunit alpha
Short name:
NAC-alpha
Alternative name(s):
Alpha-NAC
Allergen: Hom s 2
Gene namesi
Name:NACA
ORF Names:HSD48
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000196531.10
HGNCiHGNC:7629 NACA
MIMi601234 gene
neXtProtiNX_Q13765

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. Binds to IgE from atopic dermatitis (AD) patients. Identified as an IgE autoantigen in atopic dermatitis (AD) patients with severe skin manifestations.1 Publication

Keywords - Diseasei

Allergen

Organism-specific databases

DisGeNETi4666
OpenTargetsiENSG00000196531
PharmGKBiPA31433

Protein family/group databases

Allergomei3323 Hom s 2.0101
412 Hom s 2

Polymorphism and mutation databases

BioMutaiNACA
DMDMi71151996

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001355761 – 215Nascent polypeptide-associated complex subunit alphaAdd BLAST215

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei43Phosphoserine; by ILK11 Publication1
Modified residuei132PhosphoserineCombined sources1
Modified residuei142N6-acetyllysine; alternateCombined sources1
Cross-linki142Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Modified residuei159Phosphothreonine; by GSK3-betaBy similarity1
Modified residuei161PhosphothreonineCombined sources1
Modified residuei166PhosphoserineCombined sources1 Publication1
Modified residuei186PhosphoserineCombined sources1
Modified residuei191PhosphoserineCombined sources1
Modified residuei203PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylation of Thr-159 by GSK3B may promote proteasome mediated degradation (By similarity). Phosphorylation of Ser-43 by ILK during cell adhesion may promote nuclear localization.By similarity2 Publications

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ13765
MaxQBiQ13765
PeptideAtlasiQ13765
PRIDEiQ13765
ProteomicsDBi59679
TopDownProteomicsiQ13765-1 [Q13765-1]

PTM databases

iPTMnetiQ13765
SwissPalmiQ13765

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiENSG00000196531 Expressed in 241 organ(s), highest expression level in testis
CleanExiHS_NACA
ExpressionAtlasiQ13765 baseline and differential
GenevisibleiQ13765 HS

Organism-specific databases

HPAiHPA073152
HPA073648

Interactioni

Subunit structurei

Interacts with TBP and JUN (By similarity). Part of the nascent polypeptide-associated complex (NAC), which is a heterodimer of NACA and BTF3 (via NAC-A/B domains). NAC associates with ribosomes through the BTF3/NACB subunit and contacts the ribosomal protein L23, which is positioned near the exiting site. Both subunits can contact nascent polypeptide chains. NACA may also form homodimers, and only this form binds DNA.By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TXLNAP402222EBI-712216,EBI-359793

Protein-protein interaction databases

BioGridi110748, 59 interactors
DIPiDIP-43878N
IntActiQ13765, 31 interactors
MINTiQ13765

Structurei

Secondary structure

1215
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ13765
SMRiQ13765
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ13765

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini70 – 135NAC-A/BPROSITE-ProRule annotationAdd BLAST66
Domaini176 – 213UBAAdd BLAST38

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni69 – 80Required for DNA-bindingBy similarityAdd BLAST12
Regioni93 – 108RNA/DNA-bindingAdd BLAST16

Domaini

The positively charged inner surface of the NAC-A/B domain is crucial for NACA localization in the nucleus and DNA-binding. This region is blocked from binding nucleic acids in the heterodimeric complex by a helix region in the beta-subunit, it also displays much higher affinity for RNA than DNA.

Sequence similaritiesi

Belongs to the NAC-alpha family.Curated

Phylogenomic databases

eggNOGiKOG2239 Eukaryota
COG1308 LUCA
GeneTreeiENSGT00440000033468
HOVERGENiHBG082004
InParanoidiQ13765
KOiK03626
PhylomeDBiQ13765
TreeFamiTF313348

Family and domain databases

Gene3Di2.20.70.30, 1 hit
InterProiView protein in InterPro
IPR016641 EGD2/NACA
IPR038187 NAC_A/B_dom_sf
IPR002715 Nas_poly-pep-assoc_cplx_dom
PANTHERiPTHR21713 PTHR21713, 1 hit
PfamiView protein in Pfam
PF01849 NAC, 1 hit
PIRSFiPIRSF015901 NAC_alpha, 1 hit
SMARTiView protein in SMART
SM01407 NAC, 1 hit
PROSITEiView protein in PROSITE
PS51151 NAC_AB, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q13765-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MPGEATETVP ATEQELPQPQ AETGSGTESD SDESVPELEE QDSTQATTQQ
60 70 80 90 100
AQLAAAAEID EEPVSKAKQS RSEKKARKAM SKLGLRQVTG VTRVTIRKSK
110 120 130 140 150
NILFVITKPD VYKSPASDTY IVFGEAKIED LSQQAQLAAA EKFKVQGEAV
160 170 180 190 200
SNIQENTQTP TVQEESEEEE VDETGVEVKD IELVMSQANV SRAKAVRALK
210
NNSNDIVNAI MELTM
Length:215
Mass (Da):23,384
Last modified:November 1, 1996 - v1
Checksum:i05DC563A8BEF307C
GO
Isoform skNAC-2 (identifier: E9PAV3-2) [UniParc]FASTAAdd to basket
Also known as: 2
The sequence of this isoform can be found in the external entry E9PAV3.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: No experimental confirmation available.
Length:925
Mass (Da):94,681
GO
Isoform skNAC (identifier: E9PAV3-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry E9PAV3.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: No experimental confirmation available.
Length:2,078
Mass (Da):205,422
GO

Sequence cautioni

The sequence AAV83778 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti213L → S in BAD96805 (Ref. 7) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY034001 mRNA Translation: AAK57544.1
AY911673 mRNA Translation: AAX14393.1
X80909 mRNA Translation: CAA56869.1
AF054187 mRNA Translation: AAC99403.1
AK090650 mRNA Translation: BAG52208.1
AK311904 mRNA Translation: BAG34845.1
CR450295 mRNA Translation: CAG29291.1
AK223085 mRNA Translation: BAD96805.1
AC117378 Genomic DNA No translation available.
CH471054 Genomic DNA Translation: EAW96960.1
BC105120 mRNA Translation: AAI05121.1
BC105122 mRNA Translation: AAI05123.1
BC106041 mRNA Translation: AAI06042.1
AY605660 mRNA Translation: AAV83778.1 Different initiation.
CCDSiCCDS31837.1 [Q13765-1]
PIRiS49326
RefSeqiNP_001106672.1, NM_001113201.2 [Q13765-1]
NP_001106673.1, NM_001113202.1 [Q13765-1]
NP_001106674.2, NM_001113203.2
NP_001307122.1, NM_001320193.1 [Q13765-1]
NP_001307123.1, NM_001320194.1
NP_005585.1, NM_005594.5 [Q13765-1]
UniGeneiHs.505735

Genome annotation databases

EnsembliENST00000356769; ENSP00000349212; ENSG00000196531 [Q13765-1]
ENST00000393891; ENSP00000377469; ENSG00000196531 [Q13765-1]
ENST00000546392; ENSP00000446801; ENSG00000196531 [Q13765-1]
ENST00000552540; ENSP00000447821; ENSG00000196531 [Q13765-1]
GeneIDi4666
KEGGihsa:4666
UCSCiuc001sly.3 human [Q13765-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY034001 mRNA Translation: AAK57544.1
AY911673 mRNA Translation: AAX14393.1
X80909 mRNA Translation: CAA56869.1
AF054187 mRNA Translation: AAC99403.1
AK090650 mRNA Translation: BAG52208.1
AK311904 mRNA Translation: BAG34845.1
CR450295 mRNA Translation: CAG29291.1
AK223085 mRNA Translation: BAD96805.1
AC117378 Genomic DNA No translation available.
CH471054 Genomic DNA Translation: EAW96960.1
BC105120 mRNA Translation: AAI05121.1
BC105122 mRNA Translation: AAI05123.1
BC106041 mRNA Translation: AAI06042.1
AY605660 mRNA Translation: AAV83778.1 Different initiation.
CCDSiCCDS31837.1 [Q13765-1]
PIRiS49326
RefSeqiNP_001106672.1, NM_001113201.2 [Q13765-1]
NP_001106673.1, NM_001113202.1 [Q13765-1]
NP_001106674.2, NM_001113203.2
NP_001307122.1, NM_001320193.1 [Q13765-1]
NP_001307123.1, NM_001320194.1
NP_005585.1, NM_005594.5 [Q13765-1]
UniGeneiHs.505735

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3LKXX-ray2.50B84-136[»]
3MCBX-ray1.90A79-132[»]
3MCEX-ray2.40A/B/C/D81-133[»]
ProteinModelPortaliQ13765
SMRiQ13765
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110748, 59 interactors
DIPiDIP-43878N
IntActiQ13765, 31 interactors
MINTiQ13765

Protein family/group databases

Allergomei3323 Hom s 2.0101
412 Hom s 2

PTM databases

iPTMnetiQ13765
SwissPalmiQ13765

Polymorphism and mutation databases

BioMutaiNACA
DMDMi71151996

Proteomic databases

EPDiQ13765
MaxQBiQ13765
PeptideAtlasiQ13765
PRIDEiQ13765
ProteomicsDBi59679
TopDownProteomicsiQ13765-1 [Q13765-1]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000356769; ENSP00000349212; ENSG00000196531 [Q13765-1]
ENST00000393891; ENSP00000377469; ENSG00000196531 [Q13765-1]
ENST00000546392; ENSP00000446801; ENSG00000196531 [Q13765-1]
ENST00000552540; ENSP00000447821; ENSG00000196531 [Q13765-1]
GeneIDi4666
KEGGihsa:4666
UCSCiuc001sly.3 human [Q13765-1]

Organism-specific databases

CTDi4666
DisGeNETi4666
EuPathDBiHostDB:ENSG00000196531.10
GeneCardsiNACA
H-InvDBiHIX0034277
HGNCiHGNC:7629 NACA
HPAiHPA073152
HPA073648
MIMi601234 gene
neXtProtiNX_Q13765
OpenTargetsiENSG00000196531
PharmGKBiPA31433
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2239 Eukaryota
COG1308 LUCA
GeneTreeiENSGT00440000033468
HOVERGENiHBG082004
InParanoidiQ13765
KOiK03626
PhylomeDBiQ13765
TreeFamiTF313348

Enzyme and pathway databases

SIGNORiQ13765

Miscellaneous databases

ChiTaRSiNACA human
EvolutionaryTraceiQ13765
GeneWikiiNACA_(gene)
GenomeRNAii4666
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000196531 Expressed in 241 organ(s), highest expression level in testis
CleanExiHS_NACA
ExpressionAtlasiQ13765 baseline and differential
GenevisibleiQ13765 HS

Family and domain databases

Gene3Di2.20.70.30, 1 hit
InterProiView protein in InterPro
IPR016641 EGD2/NACA
IPR038187 NAC_A/B_dom_sf
IPR002715 Nas_poly-pep-assoc_cplx_dom
PANTHERiPTHR21713 PTHR21713, 1 hit
PfamiView protein in Pfam
PF01849 NAC, 1 hit
PIRSFiPIRSF015901 NAC_alpha, 1 hit
SMARTiView protein in SMART
SM01407 NAC, 1 hit
PROSITEiView protein in PROSITE
PS51151 NAC_AB, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNACA_HUMAN
AccessioniPrimary (citable) accession number: Q13765
Secondary accession number(s): A8MTN7
, B2R4P8, F8VU71, Q3KQV4, Q53A18, Q53G46
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: November 1, 1996
Last modified: November 7, 2018
This is version 167 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  5. Allergens
    Nomenclature of allergens and list of entries
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