UniProtKB - Q13761 (RUNX3_HUMAN)
Runt-related transcription factor 3
RUNX3
Functioni
Forms the heterodimeric complex core-binding factor (CBF) with CBFB. RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters (By similarity).
May be involved in the control of cellular proliferation and/or differentiation. In association with ZFHX3, up-regulates CDKN1A promoter activity following TGF-beta stimulation (PubMed:20599712).
CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation. CBF complexes binding to the transcriptional silencer is essential for recruitment of nuclear protein complexes that catalyze epigenetic modifications to establish epigenetic ZBTB7B silencing (By similarity).
By similarity1 PublicationGO - Molecular functioni
- ATP binding Source: UniProtKB
- DNA-binding transcription factor activity Source: ProtInc
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: BHF-UCL
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: BHF-UCL
- sequence-specific double-stranded DNA binding Source: ARUK-UCL
- transcription corepressor binding Source: UniProtKB
GO - Biological processi
- chondrocyte differentiation Source: GO_Central
- hemopoiesis Source: GO_Central
- negative regulation of CD4-positive, alpha-beta T cell differentiation Source: UniProtKB
- negative regulation of cell cycle Source: UniProtKB
- negative regulation of epithelial cell proliferation Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: BHF-UCL
- neuron differentiation Source: GO_Central
- ossification Source: GO_Central
- peripheral nervous system neuron development Source: BHF-UCL
- positive regulation of CD8-positive, alpha-beta T cell differentiation Source: UniProtKB
- positive regulation of transcription, DNA-templated Source: UniProtKB
- protein phosphorylation Source: UniProtKB
- regulation of cell differentiation Source: GO_Central
- regulation of transcription, DNA-templated Source: UniProtKB
- regulation of transcription by RNA polymerase II Source: GO_Central
- response to transforming growth factor beta Source: UniProtKB
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | Q13761 |
Reactomei | R-HSA-4411364, Binding of TCF/LEF:CTNNB1 to target gene promoters R-HSA-8941855, RUNX3 regulates CDKN1A transcription R-HSA-8941856, RUNX3 regulates NOTCH signaling R-HSA-8941858, Regulation of RUNX3 expression and activity R-HSA-8949275, RUNX3 Regulates Immune Response and Cell Migration R-HSA-8951430, RUNX3 regulates WNT signaling R-HSA-8951671, RUNX3 regulates YAP1-mediated transcription R-HSA-8951911, RUNX3 regulates RUNX1-mediated transcription R-HSA-8951936, RUNX3 regulates p14-ARF R-HSA-8952158, RUNX3 regulates BCL2L11 (BIM) transcription |
SignaLinki | Q13761 |
SIGNORi | Q13761 |
Names & Taxonomyi
Protein namesi | Recommended name: Runt-related transcription factor 3Alternative name(s): Acute myeloid leukemia 2 protein Core-binding factor subunit alpha-3 Short name: CBF-alpha-3 Oncogene AML-2 Polyomavirus enhancer-binding protein 2 alpha C subunit Short name: PEA2-alpha C Short name: PEBP2-alpha C SL3-3 enhancer factor 1 alpha C subunit SL3/AKV core-binding factor alpha C subunit |
Gene namesi | Name:RUNX3 Synonyms:AML2, CBFA3, PEBP2A3 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:10473, RUNX3 |
MIMi | 600210, gene |
neXtProti | NX_Q13761 |
VEuPathDBi | HostDB:ENSG00000020633 |
Subcellular locationi
Cytosol
- cytosol Source: HPA
Nucleus
- core-binding factor complex Source: UniProtKB
- nucleolus Source: HPA
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
Other locations
- chromatin Source: BHF-UCL
- cytoplasm Source: UniProtKB
- intracellular membrane-bounded organelle Source: HPA
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 864 |
OpenTargetsi | ENSG00000020633 |
PharmGKBi | PA34886 |
Miscellaneous databases
Pharosi | Q13761, Tbio |
Genetic variation databases
BioMutai | RUNX3 |
DMDMi | 17368453 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000174662 | 1 – 415 | Runt-related transcription factor 3Add BLAST | 415 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 192 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 243 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q13761 |
jPOSTi | Q13761 |
MassIVEi | Q13761 |
MaxQBi | Q13761 |
PaxDbi | Q13761 |
PeptideAtlasi | Q13761 |
PRIDEi | Q13761 |
ProteomicsDBi | 59677 [Q13761-1] 59678 [Q13761-2] |
PTM databases
GlyGeni | Q13761, 11 sites, 1 O-linked glycan (11 sites) |
iPTMneti | Q13761 |
PhosphoSitePlusi | Q13761 |
SwissPalmi | Q13761 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000020633, Expressed in granulocyte and 183 other tissues |
ExpressionAtlasi | Q13761, baseline and differential |
Genevisiblei | Q13761, HS |
Organism-specific databases
HPAi | ENSG00000020633, Tissue enhanced (bone marrow, lymphoid tissue) |
Interactioni
Subunit structurei
Heterodimer with CBFB. RUNX3 binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization (By similarity).
Interacts with TLE1 and SUV39H1 (PubMed:9751710 and PubMed:16652147). The tyrosine phosphorylated form (via runt domain) interacts with SRC (via protein kinase domain)(PubMed:20100835).
Interacts with FYN and LCK (PubMed:20100835).
Interacts with FOXP3 (PubMed:17377532).
Interacts with ZFHX3 (PubMed:20599712).
Interacts with TBX21 (By similarity).
By similarity5 PublicationsBinary interactionsi
Q13761
Isoform 2 [Q13761-2]
With | #Exp. | IntAct |
---|---|---|
CBFB [Q13951] | 3 | EBI-12145465,EBI-718750 |
GUCD1 - isoform 2 [Q96NT3-2] | 3 | EBI-12145465,EBI-11978177 |
GO - Molecular functioni
- transcription corepressor binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 107312, 55 interactors |
ELMi | Q13761 |
IntActi | Q13761, 18 interactors |
MINTi | Q13761 |
STRINGi | 9606.ENSP00000382800 |
Miscellaneous databases
RNActi | Q13761, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 54 – 182 | RuntPROSITE-ProRule annotationAdd BLAST | 129 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 48 | DisorderedSequence analysisAdd BLAST | 48 | |
Regioni | 176 – 266 | DisorderedSequence analysisAdd BLAST | 91 | |
Regioni | 375 – 415 | DisorderedSequence analysisAdd BLAST | 41 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 176 – 204 | Basic and acidic residuesSequence analysisAdd BLAST | 29 | |
Compositional biasi | 211 – 244 | Polar residuesSequence analysisAdd BLAST | 34 | |
Compositional biasi | 389 – 403 | Polar residuesSequence analysisAdd BLAST | 15 |
Domaini
Phylogenomic databases
eggNOGi | KOG3982, Eukaryota |
GeneTreei | ENSGT00940000156598 |
HOGENOMi | CLU_032910_3_0_1 |
InParanoidi | Q13761 |
OMAi | TQGLWPE |
PhylomeDBi | Q13761 |
TreeFami | TF321496 |
Family and domain databases
Gene3Di | 2.60.40.720, 1 hit |
InterProi | View protein in InterPro IPR000040, AML1_Runt IPR008967, p53-like_TF_DNA-bd IPR012346, p53/RUNT-type_TF_DNA-bd_sf IPR013524, Runt_dom IPR013711, RunxI_C_dom IPR016554, TF_Runt-rel_RUNX |
PANTHERi | PTHR11950, PTHR11950, 1 hit |
Pfami | View protein in Pfam PF00853, Runt, 1 hit PF08504, RunxI, 1 hit |
PIRSFi | PIRSF009374, TF_Runt-rel_RUNX, 1 hit |
PRINTSi | PR00967, ONCOGENEAML1 |
SUPFAMi | SSF49417, SSF49417, 1 hit |
PROSITEi | View protein in PROSITE PS51062, RUNT, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MRIPVDPSTS RRFTPPSPAF PCGGGGGKMG ENSGALSAQA AVGPGGRARP
60 70 80 90 100
EVRSMVDVLA DHAGELVRTD SPNFLCSVLP SHWRCNKTLP VAFKVVALGD
110 120 130 140 150
VPDGTVVTVM AGNDENYSAE LRNASAVMKN QVARFNDLRF VGRSGRGKSF
160 170 180 190 200
TLTITVFTNP TQVATYHRAI KVTVDGPREP RRHRQKLEDQ TKPFPDRFGD
210 220 230 240 250
LERLRMRVTP STPSPRGSLS TTSHFSSQPQ TPIQGTSELN PFSDPRQFDR
260 270 280 290 300
SFPTLPTLTE SRFPDPRMHY PGAMSAAFPY SATPSGTSIS SLSVAGMPAT
310 320 330 340 350
SRFHHTYLPP PYPGAPQNQS GPFQANPSPY HLYYGTSSGS YQFSMVAGSS
360 370 380 390 400
SGGDRSPTRM LASCTSSAAS VAAGNLMNPS LGGQSDGVEA DGSHSNSPTA
410
LSTPGRMDEA VWRPY
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 249 | D → V in CAA56093 (PubMed:7835892).Curated | 1 | |
Sequence conflicti | 253 | P → S in CAA56093 (PubMed:7835892).Curated | 1 | |
Sequence conflicti | 256 | P → S in CAA56093 (PubMed:7835892).Curated | 1 | |
Sequence conflicti | 259 | T → S in CAA56093 (PubMed:7835892).Curated | 1 | |
Sequence conflicti | 268 – 269 | MH → IY in CAA56093 (PubMed:7835892).Curated | 2 | |
Sequence conflicti | 271 | P → T in CAA56093 (PubMed:7835892).Curated | 1 | |
Sequence conflicti | 297 – 298 | MP → IS in CAA56093 (PubMed:7835892).Curated | 2 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_005949 | 1 – 5 | MRIPV → MASNSIFDSFPTYSPTFIR in isoform 2. 1 Publication | 5 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z35278 mRNA Translation: CAA84541.1 X79550 mRNA Translation: CAA56093.2 AL023096 Genomic DNA No translation available. AL445471 Genomic DNA No translation available. CH471134 Genomic DNA Translation: EAW95148.1 BC013362 mRNA Translation: AAH13362.1 U14520 Genomic DNA Translation: AAA86465.1 |
CCDSi | CCDS257.1 [Q13761-1] CCDS30633.1 [Q13761-2] |
PIRi | B55563 S60078 |
RefSeqi | NP_001026850.1, NM_001031680.2 [Q13761-2] NP_001307601.1, NM_001320672.1 [Q13761-2] NP_004341.1, NM_004350.2 [Q13761-1] XP_005246081.1, XM_005246024.4 [Q13761-2] |
Genome annotation databases
Ensembli | ENST00000308873; ENSP00000308051; ENSG00000020633 ENST00000338888; ENSP00000343477; ENSG00000020633 [Q13761-2] ENST00000399916; ENSP00000382800; ENSG00000020633 [Q13761-2] |
GeneIDi | 864 |
KEGGi | hsa:864 |
MANE-Selecti | ENST00000308873.11; ENSP00000308051.6; NM_004350.3; NP_004341.1 |
UCSCi | uc001bjq.4, human [Q13761-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z35278 mRNA Translation: CAA84541.1 X79550 mRNA Translation: CAA56093.2 AL023096 Genomic DNA No translation available. AL445471 Genomic DNA No translation available. CH471134 Genomic DNA Translation: EAW95148.1 BC013362 mRNA Translation: AAH13362.1 U14520 Genomic DNA Translation: AAA86465.1 |
CCDSi | CCDS257.1 [Q13761-1] CCDS30633.1 [Q13761-2] |
PIRi | B55563 S60078 |
RefSeqi | NP_001026850.1, NM_001031680.2 [Q13761-2] NP_001307601.1, NM_001320672.1 [Q13761-2] NP_004341.1, NM_004350.2 [Q13761-1] XP_005246081.1, XM_005246024.4 [Q13761-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5W69 | X-ray | 2.80 | I/J/K/L | 133-141 | [»] | |
SMRi | Q13761 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 107312, 55 interactors |
ELMi | Q13761 |
IntActi | Q13761, 18 interactors |
MINTi | Q13761 |
STRINGi | 9606.ENSP00000382800 |
PTM databases
GlyGeni | Q13761, 11 sites, 1 O-linked glycan (11 sites) |
iPTMneti | Q13761 |
PhosphoSitePlusi | Q13761 |
SwissPalmi | Q13761 |
Genetic variation databases
BioMutai | RUNX3 |
DMDMi | 17368453 |
Proteomic databases
EPDi | Q13761 |
jPOSTi | Q13761 |
MassIVEi | Q13761 |
MaxQBi | Q13761 |
PaxDbi | Q13761 |
PeptideAtlasi | Q13761 |
PRIDEi | Q13761 |
ProteomicsDBi | 59677 [Q13761-1] 59678 [Q13761-2] |
Protocols and materials databases
Antibodypediai | 1124, 690 antibodies from 43 providers |
DNASUi | 864 |
Genome annotation databases
Ensembli | ENST00000308873; ENSP00000308051; ENSG00000020633 ENST00000338888; ENSP00000343477; ENSG00000020633 [Q13761-2] ENST00000399916; ENSP00000382800; ENSG00000020633 [Q13761-2] |
GeneIDi | 864 |
KEGGi | hsa:864 |
MANE-Selecti | ENST00000308873.11; ENSP00000308051.6; NM_004350.3; NP_004341.1 |
UCSCi | uc001bjq.4, human [Q13761-1] |
Organism-specific databases
CTDi | 864 |
DisGeNETi | 864 |
GeneCardsi | RUNX3 |
HGNCi | HGNC:10473, RUNX3 |
HPAi | ENSG00000020633, Tissue enhanced (bone marrow, lymphoid tissue) |
MIMi | 600210, gene |
neXtProti | NX_Q13761 |
OpenTargetsi | ENSG00000020633 |
PharmGKBi | PA34886 |
VEuPathDBi | HostDB:ENSG00000020633 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3982, Eukaryota |
GeneTreei | ENSGT00940000156598 |
HOGENOMi | CLU_032910_3_0_1 |
InParanoidi | Q13761 |
OMAi | TQGLWPE |
PhylomeDBi | Q13761 |
TreeFami | TF321496 |
Enzyme and pathway databases
PathwayCommonsi | Q13761 |
Reactomei | R-HSA-4411364, Binding of TCF/LEF:CTNNB1 to target gene promoters R-HSA-8941855, RUNX3 regulates CDKN1A transcription R-HSA-8941856, RUNX3 regulates NOTCH signaling R-HSA-8941858, Regulation of RUNX3 expression and activity R-HSA-8949275, RUNX3 Regulates Immune Response and Cell Migration R-HSA-8951430, RUNX3 regulates WNT signaling R-HSA-8951671, RUNX3 regulates YAP1-mediated transcription R-HSA-8951911, RUNX3 regulates RUNX1-mediated transcription R-HSA-8951936, RUNX3 regulates p14-ARF R-HSA-8952158, RUNX3 regulates BCL2L11 (BIM) transcription |
SignaLinki | Q13761 |
SIGNORi | Q13761 |
Miscellaneous databases
BioGRID-ORCSi | 864, 6 hits in 1057 CRISPR screens |
ChiTaRSi | RUNX3, human |
GeneWikii | RUNX3 |
GenomeRNAii | 864 |
Pharosi | Q13761, Tbio |
PROi | PR:Q13761 |
RNActi | Q13761, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000020633, Expressed in granulocyte and 183 other tissues |
ExpressionAtlasi | Q13761, baseline and differential |
Genevisiblei | Q13761, HS |
Family and domain databases
Gene3Di | 2.60.40.720, 1 hit |
InterProi | View protein in InterPro IPR000040, AML1_Runt IPR008967, p53-like_TF_DNA-bd IPR012346, p53/RUNT-type_TF_DNA-bd_sf IPR013524, Runt_dom IPR013711, RunxI_C_dom IPR016554, TF_Runt-rel_RUNX |
PANTHERi | PTHR11950, PTHR11950, 1 hit |
Pfami | View protein in Pfam PF00853, Runt, 1 hit PF08504, RunxI, 1 hit |
PIRSFi | PIRSF009374, TF_Runt-rel_RUNX, 1 hit |
PRINTSi | PR00967, ONCOGENEAML1 |
SUPFAMi | SSF49417, SSF49417, 1 hit |
PROSITEi | View protein in PROSITE PS51062, RUNT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RUNX3_HUMAN | |
Accessioni | Q13761Primary (citable) accession number: Q13761 Secondary accession number(s): B1AJV5, Q12969, Q13760 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 2, 2001 |
Last sequence update: | November 1, 1999 | |
Last modified: | February 23, 2022 | |
This is version 213 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references