Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Sodium/potassium-transporting ATPase subunit alpha-4

Gene

ATP1A4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. Plays a role in sperm motility.

Catalytic activityi

ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In).

Activity regulationi

Specifically inhibited by an endogenous cardiac glycoside, ouabain.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei3844-aspartylphosphate intermediateBy similarity1
Metal bindingi723MagnesiumBy similarity1
Metal bindingi727MagnesiumBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • sodium:potassium-exchanging ATPase activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processIon transport, Potassium transport, Sodium transport, Sodium/potassium transport, Transport
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Sodium

Enzyme and pathway databases

ReactomeiR-HSA-5578775 Ion homeostasis
R-HSA-936837 Ion transport by P-type ATPases

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/potassium-transporting ATPase subunit alpha-4 (EC:3.6.3.9)
Short name:
Na(+)/K(+) ATPase alpha-4 subunit
Alternative name(s):
Sodium pump subunit alpha-4
Gene namesi
Name:ATP1A4
Synonyms:ATP1AL2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000132681.16
HGNCiHGNC:14073 ATP1A4
MIMi607321 gene
neXtProtiNX_Q13733

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 95CytoplasmicSequence analysisAdd BLAST95
Transmembranei96 – 116HelicalSequence analysisAdd BLAST21
Topological domaini117 – 139ExtracellularSequence analysisAdd BLAST23
Transmembranei140 – 160HelicalSequence analysisAdd BLAST21
Topological domaini161 – 296CytoplasmicSequence analysisAdd BLAST136
Transmembranei297 – 316HelicalSequence analysisAdd BLAST20
Topological domaini317 – 328ExtracellularSequence analysisAdd BLAST12
Transmembranei329 – 346HelicalSequence analysisAdd BLAST18
Topological domaini347 – 778CytoplasmicSequence analysisAdd BLAST432
Transmembranei779 – 798HelicalSequence analysisAdd BLAST20
Topological domaini799 – 808ExtracellularSequence analysis10
Transmembranei809 – 829HelicalSequence analysisAdd BLAST21
Topological domaini830 – 849CytoplasmicSequence analysisAdd BLAST20
Transmembranei850 – 872HelicalSequence analysisAdd BLAST23
Topological domaini873 – 924ExtracellularSequence analysisAdd BLAST52
Transmembranei925 – 944HelicalSequence analysisAdd BLAST20
Topological domaini945 – 957CytoplasmicSequence analysisAdd BLAST13
Transmembranei958 – 976HelicalSequence analysisAdd BLAST19
Topological domaini977 – 991ExtracellularSequence analysisAdd BLAST15
Transmembranei992 – 1012HelicalSequence analysisAdd BLAST21
Topological domaini1013 – 1029CytoplasmicSequence analysisAdd BLAST17

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000132681
PharmGKBiPA65

Chemistry databases

ChEMBLiCHEMBL2095186
DrugBankiDB09479 Rubidium chloride Rb-82

Polymorphism and mutation databases

BioMutaiATP1A4
DMDMi23830899

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000463031 – 1029Sodium/potassium-transporting ATPase subunit alpha-4Add BLAST1029

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei949Phosphoserine; by PKABy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ13733
MaxQBiQ13733
PaxDbiQ13733
PeptideAtlasiQ13733
PRIDEiQ13733
ProteomicsDBi59668
59669 [Q13733-2]

PTM databases

iPTMnetiQ13733
PhosphoSitePlusiQ13733
SwissPalmiQ13733

Expressioni

Tissue specificityi

Specifically expressed in testis. Found in very low levels in skeletal muscle. Expressed in mature sperm (at protein level).

Gene expression databases

BgeeiENSG00000132681 Expressed in 71 organ(s), highest expression level in right testis
CleanExiHS_ATP1A4
ExpressionAtlasiQ13733 baseline and differential
GenevisibleiQ13733 HS

Interactioni

Subunit structurei

The sodium/potassium-transporting ATPase is composed of a catalytic alpha subunit, an auxiliary non-catalytic beta subunit and an additional regulatory subunit.Curated

Protein-protein interaction databases

BioGridi106970, 11 interactors
IntActiQ13733, 1 interactor
STRINGi9606.ENSP00000357060

Structurei

3D structure databases

ProteinModelPortaliQ13733
SMRiQ13733
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni90 – 92Interaction with phosphoinositide-3 kinaseBy similarity3

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0203 Eukaryota
COG0474 LUCA
GeneTreeiENSGT00890000139334
HOVERGENiHBG004298
InParanoidiQ13733
KOiK01539
OMAiIQYTISH
OrthoDBiEOG091G01BB
PhylomeDBiQ13733
TreeFamiTF312838

Family and domain databases

CDDicd02608 P-type_ATPase_Na-K_like, 1 hit
Gene3Di3.40.1110.10, 1 hit
3.40.50.1000, 1 hit
InterProiView protein in InterPro
IPR006068 ATPase_P-typ_cation-transptr_C
IPR004014 ATPase_P-typ_cation-transptr_N
IPR023299 ATPase_P-typ_cyto_dom_N
IPR018303 ATPase_P-typ_P_site
IPR023298 ATPase_P-typ_TM_dom_sf
IPR008250 ATPase_P-typ_transduc_dom_A_sf
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR005775 P-type_ATPase_IIC
IPR001757 P_typ_ATPase
PfamiView protein in Pfam
PF00689 Cation_ATPase_C, 1 hit
PF00690 Cation_ATPase_N, 1 hit
SMARTiView protein in SMART
SM00831 Cation_ATPase_N, 1 hit
SUPFAMiSSF56784 SSF56784, 1 hit
SSF81653 SSF81653, 1 hit
SSF81660 SSF81660, 1 hit
SSF81665 SSF81665, 1 hit
TIGRFAMsiTIGR01106 ATPase-IIC_X-K, 1 hit
TIGR01494 ATPase_P-type, 2 hits
PROSITEiView protein in PROSITE
PS00154 ATPASE_E1_E2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q13733-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGLWGKKGTV APHDQSPRRR PKKGLIKKKM VKREKQKRNM EELKKEVVMD
60 70 80 90 100
DHKLTLEELS TKYSVDLTKG HSHQRAKEIL TRGGPNTVTP PPTTPEWVKF
110 120 130 140 150
CKQLFGGFSL LLWTGAILCF VAYSIQIYFN EEPTKDNLYL SIVLSVVVIV
160 170 180 190 200
TGCFSYYQEA KSSKIMESFK NMVPQQALVI RGGEKMQINV QEVVLGDLVE
210 220 230 240 250
IKGGDRVPAD LRLISAQGCK VDNSSLTGES EPQSRSPDFT HENPLETRNI
260 270 280 290 300
CFFSTNCVEG TARGIVIATG DSTVMGRIAS LTSGLAVGQT PIAAEIEHFI
310 320 330 340 350
HLITVVAVFL GVTFFALSLL LGYGWLEAII FLIGIIVANV PEGLLATVTV
360 370 380 390 400
CLTLTAKRMA RKNCLVKNLE AVETLGSTST ICSDKTGTLT QNRMTVAHMW
410 420 430 440 450
FDMTVYEADT TEEQTGKTFT KSSDTWFMLA RIAGLCNRAD FKANQEILPI
460 470 480 490 500
AKRATTGDAS ESALLKFIEQ SYSSVAEMRE KNPKVAEIPF NSTNKYQMSI
510 520 530 540 550
HLREDSSQTH VLMMKGAPER ILEFCSTFLL NGQEYSMNDE MKEAFQNAYL
560 570 580 590 600
ELGGLGERVL GFCFLNLPSS FSKGFPFNTD EINFPMDNLC FVGLISMIDP
610 620 630 640 650
PRAAVPDAVS KCRSAGIKVI MVTGDHPITA KAIAKGVGII SEGTETAEEV
660 670 680 690 700
AARLKIPISK VDASAAKAIV VHGAELKDIQ SKQLDQILQN HPEIVFARTS
710 720 730 740 750
PQQKLIIVEG CQRLGAVVAV TGDGVNDSPA LKKADIGIAM GISGSDVSKQ
760 770 780 790 800
AADMILLDDN FASIVTGVEE GRLIFDNLKK SIMYTLTSNI PEITPFLMFI
810 820 830 840 850
ILGIPLPLGT ITILCIDLGT DMVPAISLAY ESAESDIMKR LPRNPKTDNL
860 870 880 890 900
VNHRLIGMAY GQIGMIQALA GFFTYFVILA ENGFRPVDLL GIRLHWEDKY
910 920 930 940 950
LNDLEDSYGQ QWTYEQRKVV EFTCQTAFFV TIVVVQWADL IISKTRRNSL
960 970 980 990 1000
FQQGMRNKVL IFGILEETLL AAFLSYTPGM DVALRMYPLK ITWWLCAIPY
1010 1020
SILIFVYDEI RKLLIRQHPD GWVERETYY
Length:1,029
Mass (Da):114,166
Last modified:October 10, 2002 - v3
Checksum:i69958248424D2C0B
GO
Isoform 2 (identifier: Q13733-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-864: Missing.

Note: No experimental confirmation available.
Show »
Length:165
Mass (Da):19,609
Checksum:i5789093F12A8AC94
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9PRA5E9PRA5_HUMAN
Sodium/potassium-transporting ATPas...
ATP1A4
517Annotation score:

Sequence cautioni

The sequence AAQ07964 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAC05228 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti155S → W in AAA60941 (PubMed:7809153).Curated1
Sequence conflicti991 – 1017ITWWL…LLIRQ → WSFALTAQAGVKWRILGLLQ PLPPRFK in BAC05228 (PubMed:14702039).CuratedAdd BLAST27

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04837583G → D1 PublicationCorresponds to variant dbSNP:rs6427504Ensembl.1
Natural variantiVAR_048376297E → K. Corresponds to variant dbSNP:rs17368402Ensembl.1
Natural variantiVAR_048377541M → R. Corresponds to variant dbSNP:rs16831482Ensembl.1
Natural variantiVAR_048378586M → I. Corresponds to variant dbSNP:rs7528360Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0073641 – 864Missing in isoform 2. 1 PublicationAdd BLAST864

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF506797 mRNA Translation: AAQ07964.1 Different initiation.
AL121987 Genomic DNA No translation available.
AF421887 Genomic DNA Translation: AAL35818.1
AF310646
, AF430843, AF390027, AY039031 Genomic DNA Translation: AAK72396.2
AF459737 mRNA Translation: AAL66357.1
AF352828 Genomic DNA Translation: AAM20793.1
AH002997 Genomic DNA Translation: AAA60941.1
AK098076 mRNA Translation: BAC05228.1 Different initiation.
BC028297 mRNA Translation: AAH28297.1
BC094801 mRNA Translation: AAH94801.1
CCDSiCCDS1197.1 [Q13733-1]
CCDS44255.1 [Q13733-2]
RefSeqiNP_001001734.1, NM_001001734.1 [Q13733-2]
NP_653300.2, NM_144699.3 [Q13733-1]
UniGeneiHs.662219

Genome annotation databases

EnsembliENST00000368081; ENSP00000357060; ENSG00000132681 [Q13733-1]
ENST00000470705; ENSP00000433094; ENSG00000132681 [Q13733-2]
GeneIDi480
KEGGihsa:480
UCSCiuc001fve.5 human [Q13733-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF506797 mRNA Translation: AAQ07964.1 Different initiation.
AL121987 Genomic DNA No translation available.
AF421887 Genomic DNA Translation: AAL35818.1
AF310646
, AF430843, AF390027, AY039031 Genomic DNA Translation: AAK72396.2
AF459737 mRNA Translation: AAL66357.1
AF352828 Genomic DNA Translation: AAM20793.1
AH002997 Genomic DNA Translation: AAA60941.1
AK098076 mRNA Translation: BAC05228.1 Different initiation.
BC028297 mRNA Translation: AAH28297.1
BC094801 mRNA Translation: AAH94801.1
CCDSiCCDS1197.1 [Q13733-1]
CCDS44255.1 [Q13733-2]
RefSeqiNP_001001734.1, NM_001001734.1 [Q13733-2]
NP_653300.2, NM_144699.3 [Q13733-1]
UniGeneiHs.662219

3D structure databases

ProteinModelPortaliQ13733
SMRiQ13733
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106970, 11 interactors
IntActiQ13733, 1 interactor
STRINGi9606.ENSP00000357060

Chemistry databases

ChEMBLiCHEMBL2095186
DrugBankiDB09479 Rubidium chloride Rb-82

PTM databases

iPTMnetiQ13733
PhosphoSitePlusiQ13733
SwissPalmiQ13733

Polymorphism and mutation databases

BioMutaiATP1A4
DMDMi23830899

Proteomic databases

EPDiQ13733
MaxQBiQ13733
PaxDbiQ13733
PeptideAtlasiQ13733
PRIDEiQ13733
ProteomicsDBi59668
59669 [Q13733-2]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000368081; ENSP00000357060; ENSG00000132681 [Q13733-1]
ENST00000470705; ENSP00000433094; ENSG00000132681 [Q13733-2]
GeneIDi480
KEGGihsa:480
UCSCiuc001fve.5 human [Q13733-1]

Organism-specific databases

CTDi480
EuPathDBiHostDB:ENSG00000132681.16
GeneCardsiATP1A4
HGNCiHGNC:14073 ATP1A4
MIMi607321 gene
neXtProtiNX_Q13733
OpenTargetsiENSG00000132681
PharmGKBiPA65
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0203 Eukaryota
COG0474 LUCA
GeneTreeiENSGT00890000139334
HOVERGENiHBG004298
InParanoidiQ13733
KOiK01539
OMAiIQYTISH
OrthoDBiEOG091G01BB
PhylomeDBiQ13733
TreeFamiTF312838

Enzyme and pathway databases

ReactomeiR-HSA-5578775 Ion homeostasis
R-HSA-936837 Ion transport by P-type ATPases

Miscellaneous databases

ChiTaRSiATP1A4 human
GeneWikiiATP1A4
GenomeRNAii480
PROiPR:Q13733
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000132681 Expressed in 71 organ(s), highest expression level in right testis
CleanExiHS_ATP1A4
ExpressionAtlasiQ13733 baseline and differential
GenevisibleiQ13733 HS

Family and domain databases

CDDicd02608 P-type_ATPase_Na-K_like, 1 hit
Gene3Di3.40.1110.10, 1 hit
3.40.50.1000, 1 hit
InterProiView protein in InterPro
IPR006068 ATPase_P-typ_cation-transptr_C
IPR004014 ATPase_P-typ_cation-transptr_N
IPR023299 ATPase_P-typ_cyto_dom_N
IPR018303 ATPase_P-typ_P_site
IPR023298 ATPase_P-typ_TM_dom_sf
IPR008250 ATPase_P-typ_transduc_dom_A_sf
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR005775 P-type_ATPase_IIC
IPR001757 P_typ_ATPase
PfamiView protein in Pfam
PF00689 Cation_ATPase_C, 1 hit
PF00690 Cation_ATPase_N, 1 hit
SMARTiView protein in SMART
SM00831 Cation_ATPase_N, 1 hit
SUPFAMiSSF56784 SSF56784, 1 hit
SSF81653 SSF81653, 1 hit
SSF81660 SSF81660, 1 hit
SSF81665 SSF81665, 1 hit
TIGRFAMsiTIGR01106 ATPase-IIC_X-K, 1 hit
TIGR01494 ATPase_P-type, 2 hits
PROSITEiView protein in PROSITE
PS00154 ATPASE_E1_E2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAT1A4_HUMAN
AccessioniPrimary (citable) accession number: Q13733
Secondary accession number(s): Q504T2
, Q7Z4I9, Q8TBN8, Q8WXA7, Q8WXH7, Q8WY13
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: October 10, 2002
Last modified: November 7, 2018
This is version 177 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again