UniProtKB - Q13637 (RAB32_HUMAN)
Protein
Ras-related protein Rab-32
Gene
RAB32
Organism
Homo sapiens (Human)
Status
Functioni
Acts as an A-kinase anchoring protein by binding to the type II regulatory subunit of protein kinase A and anchoring it to the mitochondrion. Also involved in synchronization of mitochondrial fission (PubMed:12186851). Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis (PubMed:21255211). Plays an important role in the control of melanin production and melanosome biogenesis (PubMed:23084991). In concert with RAB38, regulates the proper trafficking of melanogenic enzymes TYR, TYRP1 and DCT/TYRP2 to melanosomes in melanocytes (By similarity).By similarity3 Publications
Activity regulationi
Regulated by a guanine nucleotide-exchange factor (GEF) and a GTPase-activating protein (GAP) and alternates between an inactive GDP-bound and an active GTP-bound form. The BLOC-3 complex composed of HPS1 and HPS4 acts as its GEF, promotes the exchange of GDP to GTP, converting it from an inactive GDP-bound form into an active GTP-bound form (By similarity). SGSM2 acts as its GAP and inactivates it by stimulating its GTPase activity (PubMed:21269460).By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 32 – 39 | GTPBy similarity | 8 | |
Nucleotide bindingi | 81 – 85 | GTPBy similarity | 5 | |
Nucleotide bindingi | 143 – 146 | GTPBy similarity | 4 |
GO - Molecular functioni
- AP-1 adaptor complex binding Source: ParkinsonsUK-UCL
- AP-3 adaptor complex binding Source: ParkinsonsUK-UCL
- BLOC-2 complex binding Source: ParkinsonsUK-UCL
- GTPase activity Source: UniProtKB
- GTP binding Source: ParkinsonsUK-UCL
- GTP-dependent protein binding Source: ParkinsonsUK-UCL
GO - Biological processi
- antigen processing and presentation Source: UniProtKB
- endosome to melanosome transport Source: ParkinsonsUK-UCL
- intracellular protein transport Source: GO_Central
- melanosome assembly Source: UniProtKB
- melanosome organization Source: GO_Central
- mitochondrion organization Source: ParkinsonsUK-UCL
- phagosome maturation Source: UniProtKB
- protein localization to membrane Source: ParkinsonsUK-UCL
- vesicle-mediated transport Source: InterPro
Keywordsi
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | Q13637 |
Reactomei | R-HSA-8873719, RAB geranylgeranylation R-HSA-8876198, RAB GEFs exchange GTP for GDP on RABs |
SIGNORi | Q13637 |
Names & Taxonomyi
Protein namesi | Recommended name: Ras-related protein Rab-32 |
Gene namesi | Name:RAB32 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:9772, RAB32 |
MIMi | 612906, gene |
neXtProti | NX_Q13637 |
VEuPathDBi | HostDB:ENSG00000118508.4 |
Subcellular locationi
Mitochondrion
- Mitochondrion 1 Publication
- Mitochondrion outer membrane 1 Publication1 Publication; Lipid-anchor 1 Publication
Other locations
- phagosome 1 Publication
- phagosome membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated
- Melanosome By similarity
- Melanosome membrane 1 Publication
Note: Recruited to phagosomes containing S.aureus or M.tuberculosis (PubMed:21255211). The BLOC-3 complex, a heterodimer of HPS1 and HPS4 promotes its membrane localization (PubMed:23084991).2 Publications
Cytosol
- cytosol Source: Reactome
Endoplasmic reticulum
- endoplasmic reticulum Source: ParkinsonsUK-UCL
Endosome
- early endosome Source: ParkinsonsUK-UCL
Golgi apparatus
- trans-Golgi network Source: GO_Central
Mitochondrion
- mitochondrial outer membrane Source: UniProtKB
- mitochondrion Source: ParkinsonsUK-UCL
Other locations
- endomembrane system Source: GO_Central
- melanosome Source: ParkinsonsUK-UCL
- melanosome membrane Source: UniProtKB
- membrane Source: ParkinsonsUK-UCL
- mitochondria-associated endoplasmic reticulum membrane Source: ParkinsonsUK-UCL
- phagocytic vesicle Source: UniProtKB
- phagocytic vesicle membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cytoplasmic vesicle, Membrane, Mitochondrion, Mitochondrion outer membranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 39 | T → N: Decreased GTP-binding activity. 1 Publication | 1 | |
Mutagenesisi | 85 | Q → L: No change in GTPase activity. 1 Publication | 1 | |
Mutagenesisi | 89 | G → T: Impairs interaction with ANKRD27; when associated with S-90 and L-94. 1 Publication | 1 | |
Mutagenesisi | 90 | N → S: Impairs interaction with ANKRD27; when associated with T-89 and L-94. 1 Publication | 1 | |
Mutagenesisi | 91 | M → S: Impairs interaction with ANKRD27; when associated with S-93. 1 Publication | 1 | |
Mutagenesisi | 93 | R → S: Impairs interaction with ANKRD27; when associated with M-91. 1 Publication | 1 | |
Mutagenesisi | 94 | V → L: Impairs interaction with ANKRD27; when associated with T-89 and S-90. 1 Publication | 1 | |
Mutagenesisi | 185 | A → F: Abolishes binding to protein kinase A type II regulatory subunit. 1 Publication | 1 | |
Mutagenesisi | 188 | L → P: Abolishes binding to protein kinase A type II regulatory subunit. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 10981 |
OpenTargetsi | ENSG00000118508 |
PharmGKBi | PA34123 |
Miscellaneous databases
Pharosi | Q13637, Tbio |
Genetic variation databases
BioMutai | RAB32 |
DMDMi | 2833245 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources2 Publications | |||
ChainiPRO_0000121235 | 2 – 225 | Ras-related protein Rab-32Add BLAST | 224 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources2 Publications | 1 | |
Modified residuei | 71 | PhosphoserineCombined sources | 1 | |
Lipidationi | 224 | S-geranylgeranyl cysteineBy similarity | 1 | |
Lipidationi | 225 | S-geranylgeranyl cysteineBy similarity | 1 |
Keywords - PTMi
Acetylation, Lipoprotein, Phosphoprotein, PrenylationProteomic databases
EPDi | Q13637 |
jPOSTi | Q13637 |
MassIVEi | Q13637 |
MaxQBi | Q13637 |
PaxDbi | Q13637 |
PeptideAtlasi | Q13637 |
PRIDEi | Q13637 |
ProteomicsDBi | 59632 |
PTM databases
iPTMneti | Q13637 |
PhosphoSitePlusi | Q13637 |
Expressioni
Tissue specificityi
Widely expressed with high levels in heart, liver, kidney, bone marrow, testis, colon and fetal lung.2 Publications
Gene expression databases
Bgeei | ENSG00000118508, Expressed in smooth muscle tissue and 211 other tissues |
Genevisiblei | Q13637, HS |
Organism-specific databases
HPAi | ENSG00000118508, Tissue enhanced (bone) |
Interactioni
Subunit structurei
Binary interactionsi
Hide detailsQ13637
With | #Exp. | IntAct |
---|---|---|
DDIT4L [Q96D03] | 3 | EBI-9837586,EBI-742054 |
LRMDA [A0A087WWI0] | 3 | EBI-9837586,EBI-18393842 |
LRRK2 [Q5S007] | 12 | EBI-9837586,EBI-5323863 |
GO - Molecular functioni
- GTP-dependent protein binding Source: ParkinsonsUK-UCL
Protein-protein interaction databases
BioGRIDi | 116177, 42 interactors |
DIPi | DIP-61889N |
IntActi | Q13637, 38 interactors |
MINTi | Q13637 |
STRINGi | 9606.ENSP00000356465 |
Miscellaneous databases
RNActi | Q13637, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q13637 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 178 – 197 | PKA-RII subunit binding domainAdd BLAST | 20 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 54 – 62 | Effector regionBy similarity | 9 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG4423, Eukaryota |
GeneTreei | ENSGT00940000162477 |
HOGENOMi | CLU_041217_10_6_1 |
InParanoidi | Q13637 |
OMAi | NIHIDDA |
OrthoDBi | 1240760at2759 |
PhylomeDBi | Q13637 |
TreeFami | TF324491 |
Family and domain databases
CDDi | cd04107, Rab32_Rab38, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR030697, Rab29/Rab38/Rab32 IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51419, RAB, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q13637-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAGGGAGDPG LGAAAAPAPE TREHLFKVLV IGELGVGKTS IIKRYVHQLF
60 70 80 90 100
SQHYRATIGV DFALKVLNWD SRTLVRLQLW DIAGQERFGN MTRVYYKEAV
110 120 130 140 150
GAFVVFDISR SSTFEAVLKW KSDLDSKVHL PNGSPIPAVL LANKCDQNKD
160 170 180 190 200
SSQSPSQVDQ FCKEHGFAGW FETSAKDNIN IEEAARFLVE KILVNHQSFP
210 220
NEENDVDKIK LDQETLRAEN KSQCC
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U71127 mRNA Translation: AAB09599.1 AF498958 mRNA Translation: AAM21106.1 BT020016 mRNA Translation: AAV38819.1 AL133539 Genomic DNA No translation available. BC015061 mRNA Translation: AAH15061.1 U59878 mRNA Translation: AAB02833.1 |
CCDSi | CCDS5210.1 |
RefSeqi | NP_006825.1, NM_006834.4 |
Genome annotation databases
Ensembli | ENST00000367495; ENSP00000356465; ENSG00000118508 |
GeneIDi | 10981 |
KEGGi | hsa:10981 |
UCSCi | uc003qln.2, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U71127 mRNA Translation: AAB09599.1 AF498958 mRNA Translation: AAM21106.1 BT020016 mRNA Translation: AAV38819.1 AL133539 Genomic DNA No translation available. BC015061 mRNA Translation: AAH15061.1 U59878 mRNA Translation: AAB02833.1 |
CCDSi | CCDS5210.1 |
RefSeqi | NP_006825.1, NM_006834.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4CYM | X-ray | 2.80 | A/B/C | 1-225 | [»] | |
4CZ2 | X-ray | 2.97 | A/B/C | 1-225 | [»] | |
5OEC | X-ray | 2.30 | B | 20-201 | [»] | |
5OED | X-ray | 2.90 | B | 20-201 | [»] | |
6FF8 | X-ray | 2.13 | A/B | 20-198 | [»] | |
SMRi | Q13637 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 116177, 42 interactors |
DIPi | DIP-61889N |
IntActi | Q13637, 38 interactors |
MINTi | Q13637 |
STRINGi | 9606.ENSP00000356465 |
PTM databases
iPTMneti | Q13637 |
PhosphoSitePlusi | Q13637 |
Genetic variation databases
BioMutai | RAB32 |
DMDMi | 2833245 |
Proteomic databases
EPDi | Q13637 |
jPOSTi | Q13637 |
MassIVEi | Q13637 |
MaxQBi | Q13637 |
PaxDbi | Q13637 |
PeptideAtlasi | Q13637 |
PRIDEi | Q13637 |
ProteomicsDBi | 59632 |
Protocols and materials databases
Antibodypediai | 19849, 169 antibodies |
DNASUi | 10981 |
Genome annotation databases
Ensembli | ENST00000367495; ENSP00000356465; ENSG00000118508 |
GeneIDi | 10981 |
KEGGi | hsa:10981 |
UCSCi | uc003qln.2, human |
Organism-specific databases
CTDi | 10981 |
DisGeNETi | 10981 |
GeneCardsi | RAB32 |
HGNCi | HGNC:9772, RAB32 |
HPAi | ENSG00000118508, Tissue enhanced (bone) |
MIMi | 612906, gene |
neXtProti | NX_Q13637 |
OpenTargetsi | ENSG00000118508 |
PharmGKBi | PA34123 |
VEuPathDBi | HostDB:ENSG00000118508.4 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4423, Eukaryota |
GeneTreei | ENSGT00940000162477 |
HOGENOMi | CLU_041217_10_6_1 |
InParanoidi | Q13637 |
OMAi | NIHIDDA |
OrthoDBi | 1240760at2759 |
PhylomeDBi | Q13637 |
TreeFami | TF324491 |
Enzyme and pathway databases
PathwayCommonsi | Q13637 |
Reactomei | R-HSA-8873719, RAB geranylgeranylation R-HSA-8876198, RAB GEFs exchange GTP for GDP on RABs |
SIGNORi | Q13637 |
Miscellaneous databases
BioGRID-ORCSi | 10981, 1 hit in 876 CRISPR screens |
GenomeRNAii | 10981 |
Pharosi | Q13637, Tbio |
PROi | PR:Q13637 |
RNActi | Q13637, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000118508, Expressed in smooth muscle tissue and 211 other tissues |
Genevisiblei | Q13637, HS |
Family and domain databases
CDDi | cd04107, Rab32_Rab38, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR030697, Rab29/Rab38/Rab32 IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51419, RAB, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RAB32_HUMAN | |
Accessioni | Q13637Primary (citable) accession number: Q13637 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 10, 2021 | |
This is version 186 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 6
Human chromosome 6: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families