UniProtKB - Q13618 (CUL3_HUMAN)
Cullin-3
CUL3
Functioni
Core component of multiple cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. BCR complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins (PubMed:27565346).
As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the BCR complex depends on the BTB domain-containing protein as the substrate recognition component. BCR(KLHL42) is involved in ubiquitination of KATNA1. BCR(SPOP) is involved in ubiquitination of BMI1/PCGF4, BRMS1, MACROH2A1 and DAXX, GLI2 and GLI3. Can also form a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex containing homodimeric SPOPL or the heterodimer formed by SPOP and SPOPL; these complexes have lower ubiquitin ligase activity. BCR(KLHL9-KLHL13) controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis. BCR(KLHL12) is involved in ER-Golgi transport by regulating the size of COPII coats, thereby playing a key role in collagen export, which is required for embryonic stem (ES) cells division: BCR(KLHL12) acts by mediating monoubiquitination of SEC31 (SEC31A or SEC31B) (PubMed:22358839, PubMed:27716508).
BCR(KLHL3) acts as a regulator of ion transport in the distal nephron; by mediating ubiquitination of WNK4 (PubMed:23387299, PubMed:23453970, PubMed:23576762).
The BCR(KLHL20) E3 ubiquitin ligase complex is involved in interferon response and anterograde Golgi to endosome transport: it mediates both ubiquitination leading to degradation and 'Lys-33'-linked ubiquitination (PubMed:20389280, PubMed:21840486, PubMed:21670212, PubMed:24768539).
The BCR(KLHL21) E3 ubiquitin ligase complex regulates localization of the chromosomal passenger complex (CPC) from chromosomes to the spindle midzone in anaphase and mediates the ubiquitination of AURKB (PubMed:19995937).
The BCR(KLHL22) ubiquitin ligase complex mediates monoubiquitination of PLK1, leading to PLK1 dissociation from phosphoreceptor proteins and subsequent removal from kinetochores, allowing silencing of the spindle assembly checkpoint (SAC) and chromosome segregation (PubMed:23455478).
The BCR(KLHL22) ubiquitin ligase complex is also responsible for the amino acid-stimulated 'Lys-48' polyubiquitination and proteasomal degradation of DEPDC5. Through the degradation of DEPDC5, releases the GATOR1 complex-mediated inhibition of the TORC1 pathway (PubMed:29769719).
The BCR(KLHL25) ubiquitin ligase complex is involved in translational homeostasis by mediating ubiquitination and subsequent degradation of hypophosphorylated EIF4EBP1 (4E-BP1) (PubMed:22578813).
The BCR(KBTBD8) complex acts by mediating monoubiquitination of NOLC1 and TCOF1, leading to remodel the translational program of differentiating cells in favor of neural crest specification (PubMed:26399832).
Involved in ubiquitination of cyclin E and of cyclin D1 (in vitro) thus involved in regulation of G1/S transition. Involved in the ubiquitination of KEAP1, ENC1 and KLHL41 (PubMed:15983046).
In concert with ATF2 and RBX1, promotes degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. The BCR(KCTD17) E3 ubiquitin ligase complex mediates ubiquitination and degradation of TCHP, a down-regulator of cilium assembly, thereby inducing ciliogenesis (PubMed:25270598).
The BCR(KLHL24) E3 ubiquitin ligase complex mediates ubiquitination of KRT14, controls KRT14 levels during keratinocytes differentiation, and is essential for skin integrity (PubMed:27798626).
The BCR(KLHL18) E3 ubiquitin ligase complex mediates the ubiquitination of AURKA leading to its activation at the centrosome which is required for initiating mitotic entry (PubMed:23213400).
The BCR(KEAP1) E3 ubiquitin ligase complex acts as a key sensor of oxidative and electrophilic stress by mediating ubiquitination and degradation of NFE2L2/NRF2, a transcription factor regulating expression of many cytoprotective genes (PubMed:15601839, PubMed:16006525).
As part of the CUL3(KBTBD6/7) E3 ubiquitin ligase complex functions mediates 'Lys-48' ubiquitination and proteasomal degradation of TIAM1 (PubMed:25684205).
By controlling the ubiquitination of that RAC1 guanine exchange factors (GEF), regulates RAC1 signal transduction and downstream biological processes including the organization of the cytoskeleton, cell migration and cell proliferation (PubMed:25684205).
31 Publications: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
GO - Molecular functioni
- cyclin binding Source: Ensembl
- identical protein binding Source: Ensembl
- Notch binding Source: MGI
- POZ domain binding Source: UniProtKB
- ubiquitin protein ligase activity Source: UniProtKB
- ubiquitin protein ligase binding Source: UniProtKB
GO - Biological processi
- anaphase-promoting complex-dependent catabolic process Source: MGI
- cell migration Source: UniProtKB
- cell projection organization Source: UniProtKB-KW
- COPII vesicle coating Source: UniProtKB
- embryonic cleavage Source: UniProtKB
- endoplasmic reticulum to Golgi vesicle-mediated transport Source: UniProtKB
- fibroblast apoptotic process Source: Ensembl
- G1/S transition of mitotic cell cycle Source: ProtInc
- gastrulation Source: Ensembl
- integrin-mediated signaling pathway Source: UniProtKB
- intrinsic apoptotic signaling pathway Source: ProtInc
- liver morphogenesis Source: Ensembl
- mitotic metaphase plate congression Source: UniProtKB
- negative regulation of Rho protein signal transduction Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: Ensembl
- nuclear protein quality control by the ubiquitin-proteasome system Source: UniProtKB
- positive regulation of cell population proliferation Source: ProtInc
- positive regulation of cytokinesis Source: UniProtKB
- positive regulation of mitotic cell cycle phase transition Source: UniProtKB
- positive regulation of mitotic metaphase/anaphase transition Source: UniProtKB
- positive regulation of protein ubiquitination Source: BHF-UCL
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: UniProtKB
- protein autoubiquitination Source: UniProtKB
- protein destabilization Source: BHF-UCL
- protein K48-linked ubiquitination Source: UniProtKB
- protein monoubiquitination Source: UniProtKB
- protein polyubiquitination Source: UniProtKB
- protein ubiquitination Source: UniProtKB
- stem cell division Source: UniProtKB
- stress fiber assembly Source: UniProtKB
- trophectodermal cellular morphogenesis Source: Ensembl
- ubiquitin-dependent protein catabolic process Source: UniProtKB
- Wnt signaling pathway Source: Ensembl
Keywordsi
Biological process | Cell cycle, Cell division, Cilium biogenesis/degradation, ER-Golgi transport, Mitosis, Transport, Ubl conjugation pathway |
Enzyme and pathway databases
PathwayCommonsi | Q13618 |
Reactomei | R-HSA-4641258, Degradation of DVL R-HSA-5632684, Hedgehog 'on' state R-HSA-5658442, Regulation of RAS by GAPs R-HSA-8932339, ROS sensing by NFE2L2 R-HSA-8951664, Neddylation R-HSA-9013418, RHOBTB2 GTPase cycle R-HSA-9013422, RHOBTB1 GTPase cycle R-HSA-9679191, Potential therapeutics for SARS R-HSA-9706019, RHOBTB3 ATPase cycle R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
SignaLinki | Q13618 |
SIGNORi | Q13618 |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: Cullin-3CuratedShort name: CUL-3 |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:2553, CUL3 |
MIMi | 603136, gene |
neXtProti | NX_Q13618 |
VEuPathDBi | HostDB:ENSG00000036257 |
Subcellular locationi
Cytoplasm and Cytosol
Nucleus
- Nucleus 3 Publications
Golgi apparatus
- Golgi apparatus 1 Publication
Cytoskeleton
- spindle 1 Publication
- centrosome 1 Publication
- spindle pole 1 Publication
Other locations
- flagellum 1 Publication
Note: Detected along the length of the sperm flagellum and in the cytoplasm of the germ cells (PubMed:28395323). Predominantly found in the nucleus in interphase cells, found at the centrosome at late G2 or prophase, starts accumulating at the spindle poles in prometaphase and stays on the spindle poles and the mitotic spindle at metaphase (PubMed:23213400).2 Publications
Cytoskeleton
- centrosome Source: UniProtKB
- mitotic spindle Source: UniProtKB
- polar microtubule Source: UniProtKB
- spindle pole Source: UniProtKB
Cytosol
- cytosol Source: Reactome
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Golgi apparatus
- Golgi apparatus Source: UniProtKB-SubCell
Nucleus
- nucleoplasm Source: Reactome
- nucleus Source: UniProtKB
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
- Cul3-RING ubiquitin ligase complex Source: UniProtKB
- cullin-RING ubiquitin ligase complex Source: GO_Central
- cytoplasm Source: UniProtKB
- membrane Source: UniProtKB
- sperm flagellum Source: UniProtKB
Keywords - Cellular componenti
Cell projection, Cilium, Cytoplasm, Cytoskeleton, Flagellum, Golgi apparatus, NucleusPathology & Biotechi
Involvement in diseasei
Pseudohypoaldosteronism 2E (PHA2E)1 Publication
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_067532 | 413 | D → G in PHA2E. 1 PublicationCorresponds to variant dbSNP:rs199469656EnsemblClinVar. | 1 | |
Natural variantiVAR_067533 | 459 | K → R in PHA2E. 1 PublicationCorresponds to variant dbSNP:rs199469658EnsemblClinVar. | 1 |
Neurodevelopmental disorder with or without autism or seizures (NEDAUS)2 Publications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_085407 | 285 | V → A in NEDAUS; decreases interaction with KEAP1. 1 Publication | 1 | |
Natural variantiVAR_085408 | 587 – 768 | Missing in NEDAUS. 1 PublicationAdd BLAST | 182 |
Keywords - Diseasei
Autism spectrum disorder, Disease variant, Epilepsy, Mental retardationOrganism-specific databases
DisGeNETi | 8452 |
GeneReviewsi | CUL3 |
MalaCardsi | CUL3 |
MIMi | 614496, phenotype 619239, phenotype |
OpenTargetsi | ENSG00000036257 |
Orphaneti | 300530, Pseudohypoaldosteronism type 2E |
PharmGKBi | PA27049 |
Miscellaneous databases
Pharosi | Q13618, Tbio |
Genetic variation databases
BioMutai | CUL3 |
DMDMi | 12643396 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000119793 | 2 – 768 | Cullin-3Add BLAST | 767 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources | 1 | |
Modified residuei | 585 | PhosphoserineCombined sources | 1 | |
Cross-linki | 712 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in NEDD8)By similarity |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q13618 |
jPOSTi | Q13618 |
MassIVEi | Q13618 |
MaxQBi | Q13618 |
PaxDbi | Q13618 |
PeptideAtlasi | Q13618 |
PRIDEi | Q13618 |
ProteomicsDBi | 59606 [Q13618-1] 59607 [Q13618-2] 59608 [Q13618-3] |
PTM databases
iPTMneti | Q13618 |
PhosphoSitePlusi | Q13618 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000036257, Expressed in sperm and 241 other tissues |
ExpressionAtlasi | Q13618, baseline and differential |
Genevisiblei | Q13618, HS |
Organism-specific databases
HPAi | ENSG00000036257, Tissue enhanced (testis) |
Interactioni
Subunit structurei
Forms neddylation-dependent homodimers.
Component of multiple BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes formed of CUL3, RBX1 and a variable BTB domain-containing protein acting as both, adapter to cullin and substrate recognition subunit. The BCR complex may be active as a heterodimeric complex, in which NEDD8, covalently attached to one CUL3 molecule, binds to the C-terminus of a second CUL3 molecule.
Interacts with RBX1, RNF7, CYCE and TIP120A/CAND1 (PubMed:10500095, PubMed:10230407, PubMed:12609982). Part of the BCR(SPOP) containing SPOP, and of BCR containing homodimeric SPOPL or the heterodimer formed by SPOP and SPOPL. Part of the probable BCR(KLHL9-KLHL13) complex with BTB domain proteins KLHL9 and KLHL13. Part of the BCR(KLHL41) complex containing KLHL41.
Component of the BCR(KLHL12) E3 ubiquitin ligase complex, at least composed of CUL3 and KLHL12 and RBX1.
Component of the BCR(KLHL3) E3 ubiquitin ligase complex, at least composed of CUL3 and KLHL3 and RBX1 (Probable). Part of the BCR(ENC1) complex containing ENC1.
Part of a complex consisting of BMI1/PCGF4, CUL3 and SPOP.
Part of a complex consisting of BRMS1, CUL3 and SPOP.
Component of the BCR(KLHL21) E3 ubiquitin ligase complex, at least composed of CUL3, KLHL21 and RBX1.
Component of the BCR(KLHL22) E3 ubiquitin ligase complex, at least composed of CUL3, KLHL22 and RBX1.
Component of the BCR(KLHL25) E3 ubiquitin ligase complex, at least composed of CUL3, KLHL25 and RBX1.
Part of a complex consisting of MACROH2A1, CUL3 and SPOP.
Component of the BCR(KLHL42) E3 ubiquitin ligase complex, at least composed of CUL3 and KLHL42.
Interacts with KLHL42 (via the BTB domain).
Interacts with KATNA1; the interaction is enhanced by KLHL42.
Component of the BCR(KBTBD8) E3 ubiquitin ligase complex, at least composed of CUL3, KBTBD8 and RBX1 (PubMed:26399832).
Interacts with KCTD5, KLHL9, KLHL11, KLHL13, GAN, ZBTB16, KLHL3, KLHL15, KLHL20, KLHL36, GMCL2, BTBD1.
Part of a complex that contains CUL3, RBX1 and GAN.
Interacts (via BTB domain) with KLHL17; the interaction regulates surface GRIK2 expression.
Interacts with KCTD7. Part of the BCR(GAN) complex containing GAN. Part of the BCR(KEAP1) complex containing KEAP1 (PubMed:15983046, PubMed:15601839, PubMed:32341456).
Interacts with KLHL10 (By similarity).
Interacts with KAT5 and ATF2.
Interacts with KCTD17 in the BCR(KCTD17) E3 ubiquitin ligase complex, at least composed of CUL3, KCTD17 and RBX1 (PubMed:25270598).
Interacts (when neddylated) with ARIH1; leading to activate the E3 ligase activity of ARIH1 (PubMed:24076655, PubMed:27565346).
Interacts with COPS9 isoform 2 (PubMed:23776465).
Interacts with PPP2R5B; this interaction is indirect and mediated through KLHL15-binding and leads to PPP2R5B proteasomal degradation (PubMed:23135275).
Interacts with RBBP8/CtIP; this interaction is indirect and mediated through KLHL15-binding and leads to RBBP8 proteasomal degradation (PubMed:27561354).
Interacts with KLHL24 in the BCR(KLHL24) E3 ubiquitin ligase complex, composed of CUL3, RBX1 and KLHL24 (PubMed:27798626).
Interacts with RHOBTB2 (PubMed:29276004).
Interacts with AURKA and KLHL18 (via BTB domain) (PubMed:23213400).
Interacts (unneddylated form) with DCUN1D1, DCUN1D2, DCUN1D3, DCUN1D4 and DCUN1D5; these interactions promote the cullin neddylation (PubMed:24192928, PubMed:26906416, PubMed:23201271, PubMed:25349211).
Component of a BCR3 (BTB-CUL3-RBX1) E3 ubiquitin ligase complex, also named Cul3-RING ubiquitin ligase complex CUL3(KBTBD6/7), composed of CUL3, RBX1, KBTBD6 and KBTBD7 (PubMed:25684205).
By similarityCurated44 PublicationsBinary interactionsi
Q13618
Isoform 1 [Q13618-1]
With | #Exp. | IntAct |
---|---|---|
DCUN1D3 [Q8IWE4] | 3 | EBI-15794202,EBI-15794102 |
GO - Molecular functioni
- cyclin binding Source: Ensembl
- identical protein binding Source: Ensembl
- Notch binding Source: MGI
- POZ domain binding Source: UniProtKB
- ubiquitin protein ligase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 114030, 1296 interactors |
CORUMi | Q13618 |
DIPi | DIP-31611N |
IntActi | Q13618, 1057 interactors |
MINTi | Q13618 |
STRINGi | 9606.ENSP00000264414 |
Miscellaneous databases
RNActi | Q13618, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q13618 |
SMRi | Q13618 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 2 – 41 | Interaction with KLHL181 PublicationAdd BLAST | 40 | |
Regioni | 677 – 698 | DisorderedSequence analysisAdd BLAST | 22 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 682 – 698 | Basic and acidic residuesSequence analysisAdd BLAST | 17 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2166, Eukaryota |
GeneTreei | ENSGT00940000155066 |
HOGENOMi | CLU_004747_7_1_1 |
InParanoidi | Q13618 |
OMAi | MFKDMTI |
OrthoDBi | 1040292at2759 |
PhylomeDBi | Q13618 |
TreeFami | TF105858 |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR045093, Cullin IPR016157, Cullin_CS IPR016158, Cullin_homology IPR036317, Cullin_homology_sf IPR001373, Cullin_N IPR019559, Cullin_neddylation_domain IPR016159, Cullin_repeat-like_dom_sf IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR11932, PTHR11932, 1 hit |
Pfami | View protein in Pfam PF00888, Cullin, 1 hit PF10557, Cullin_Nedd8, 1 hit |
SMARTi | View protein in SMART SM00182, CULLIN, 1 hit SM00884, Cullin_Nedd8, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF74788, SSF74788, 1 hit SSF75632, SSF75632, 1 hit |
PROSITEi | View protein in PROSITE PS01256, CULLIN_1, 1 hit PS50069, CULLIN_2, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSNLSKGTGS RKDTKMRIRA FPMTMDEKYV NSIWDLLKNA IQEIQRKNNS
60 70 80 90 100
GLSFEELYRN AYTMVLHKHG EKLYTGLREV VTEHLINKVR EDVLNSLNNN
110 120 130 140 150
FLQTLNQAWN DHQTAMVMIR DILMYMDRVY VQQNNVENVY NLGLIIFRDQ
160 170 180 190 200
VVRYGCIRDH LRQTLLDMIA RERKGEVVDR GAIRNACQML MILGLEGRSV
210 220 230 240 250
YEEDFEAPFL EMSAEFFQME SQKFLAENSA SVYIKKVEAR INEEIERVMH
260 270 280 290 300
CLDKSTEEPI VKVVERELIS KHMKTIVEME NSGLVHMLKN GKTEDLGCMY
310 320 330 340 350
KLFSRVPNGL KTMCECMSSY LREQGKALVS EEGEGKNPVD YIQGLLDLKS
360 370 380 390 400
RFDRFLLESF NNDRLFKQTI AGDFEYFLNL NSRSPEYLSL FIDDKLKKGV
410 420 430 440 450
KGLTEQEVET ILDKAMVLFR FMQEKDVFER YYKQHLARRL LTNKSVSDDS
460 470 480 490 500
EKNMISKLKT ECGCQFTSKL EGMFRDMSIS NTTMDEFRQH LQATGVSLGG
510 520 530 540 550
VDLTVRVLTT GYWPTQSATP KCNIPPAPRH AFEIFRRFYL AKHSGRQLTL
560 570 580 590 600
QHHMGSADLN ATFYGPVKKE DGSEVGVGGA QVTGSNTRKH ILQVSTFQMT
610 620 630 640 650
ILMLFNNREK YTFEEIQQET DIPERELVRA LQSLACGKPT QRVLTKEPKS
660 670 680 690 700
KEIENGHIFT VNDQFTSKLH RVKIQTVAAK QGESDPERKE TRQKVDDDRK
710 720 730 740 750
HEIEAAIVRI MKSRKKMQHN VLVAEVTQQL KARFLPSPVV IKKRIEGLIE
760
REYLARTPED RKVYTYVA
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH7C399 | H7C399_HUMAN | Cullin-3 | CUL3 | 111 | Annotation score: | ||
H7C1L6 | H7C1L6_HUMAN | Cullin-3 | CUL3 | 192 | Annotation score: | ||
A0A087X1R9 | A0A087X1R9_HUMAN | Cullin-3 | CUL3 | 55 | Annotation score: | ||
H7C1J0 | H7C1J0_HUMAN | Cullin-3 | CUL3 | 43 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 13 | D → G in AAC36682 (PubMed:9663463).Curated | 1 | |
Sequence conflicti | 397 | K → T in AAQ01660 (Ref. 4) Curated | 1 | |
Sequence conflicti | 481 | N → T in AAQ01660 (Ref. 4) Curated | 1 | |
Sequence conflicti | 609 | E → G in AAH31844 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 666 | T → I in AAQ01660 (Ref. 4) Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_017194 | 13 | D → H. Corresponds to variant dbSNP:rs2969802Ensembl. | 1 | |
Natural variantiVAR_048839 | 184 | R → S. Corresponds to variant dbSNP:rs17480168Ensembl. | 1 | |
Natural variantiVAR_085407 | 285 | V → A in NEDAUS; decreases interaction with KEAP1. 1 Publication | 1 | |
Natural variantiVAR_067532 | 413 | D → G in PHA2E. 1 PublicationCorresponds to variant dbSNP:rs199469656EnsemblClinVar. | 1 | |
Natural variantiVAR_067533 | 459 | K → R in PHA2E. 1 PublicationCorresponds to variant dbSNP:rs199469658EnsemblClinVar. | 1 | |
Natural variantiVAR_017195 | 567 | V → I. Corresponds to variant dbSNP:rs3738952EnsemblClinVar. | 1 | |
Natural variantiVAR_085408 | 587 – 768 | Missing in NEDAUS. 1 PublicationAdd BLAST | 182 | |
Natural variantiVAR_078688 | 719 | H → R Found in a patient with autism spectrum disorder; unknown pathological significance. 1 PublicationCorresponds to variant dbSNP:rs763087632Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_008824 | 1 – 24 | Missing in isoform 2. 1 PublicationAdd BLAST | 24 | |
Alternative sequenceiVSP_008825 | 23 – 88 | Missing in isoform 3. CuratedAdd BLAST | 66 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF064087 mRNA Translation: AAC36304.1 AB014517 mRNA Translation: BAA31592.2 Different initiation. AF062537 mRNA Translation: AAC36682.1 Frameshift. AY337761 mRNA Translation: AAQ01660.1 AK291151 mRNA Translation: BAF83840.1 AC073052 Genomic DNA No translation available. AC092679 Genomic DNA No translation available. CH471063 Genomic DNA Translation: EAW70828.1 BC031844 mRNA Translation: AAH31844.1 BC039598 mRNA Translation: AAH39598.1 BC092409 mRNA Translation: AAH92409.1 U58089 mRNA Translation: AAC50546.1 AF052147 mRNA Translation: AAC28621.1 Frameshift. |
CCDSi | CCDS2462.1 [Q13618-1] CCDS58751.1 [Q13618-3] |
RefSeqi | NP_001244126.1, NM_001257197.1 [Q13618-3] NP_001244127.1, NM_001257198.1 NP_003581.1, NM_003590.4 [Q13618-1] |
Genome annotation databases
Ensembli | ENST00000264414.9; ENSP00000264414.4; ENSG00000036257.14 ENST00000344951.8; ENSP00000343601.4; ENSG00000036257.14 [Q13618-3] ENST00000409096.5; ENSP00000387200.1; ENSG00000036257.14 [Q13618-2] ENST00000409777.5; ENSP00000386525.1; ENSG00000036257.14 [Q13618-2] |
GeneIDi | 8452 |
KEGGi | hsa:8452 |
MANE-Selecti | ENST00000264414.9; ENSP00000264414.4; NM_003590.5; NP_003581.1 |
UCSCi | uc002vny.4, human [Q13618-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF064087 mRNA Translation: AAC36304.1 AB014517 mRNA Translation: BAA31592.2 Different initiation. AF062537 mRNA Translation: AAC36682.1 Frameshift. AY337761 mRNA Translation: AAQ01660.1 AK291151 mRNA Translation: BAF83840.1 AC073052 Genomic DNA No translation available. AC092679 Genomic DNA No translation available. CH471063 Genomic DNA Translation: EAW70828.1 BC031844 mRNA Translation: AAH31844.1 BC039598 mRNA Translation: AAH39598.1 BC092409 mRNA Translation: AAH92409.1 U58089 mRNA Translation: AAC50546.1 AF052147 mRNA Translation: AAC28621.1 Frameshift. |
CCDSi | CCDS2462.1 [Q13618-1] CCDS58751.1 [Q13618-3] |
RefSeqi | NP_001244126.1, NM_001257197.1 [Q13618-3] NP_001244127.1, NM_001257198.1 NP_003581.1, NM_003590.4 [Q13618-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2MYL | NMR | - | A | 49-68 | [»] | |
2MYM | NMR | - | A | 49-68 | [»] | |
4AP2 | X-ray | 2.80 | B | 1-388 | [»] | |
4APF | X-ray | 3.10 | B | 23-388 | [»] | |
4EOZ | X-ray | 2.40 | B/D | 20-381 | [»] | |
4HXI | X-ray | 3.51 | B | 20-381 | [»] | |
5NLB | X-ray | 3.45 | B | 26-381 | [»] | |
6I2M | X-ray | 2.30 | B | 23-388 | [»] | |
AlphaFoldDBi | Q13618 | |||||
SMRi | Q13618 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 114030, 1296 interactors |
CORUMi | Q13618 |
DIPi | DIP-31611N |
IntActi | Q13618, 1057 interactors |
MINTi | Q13618 |
STRINGi | 9606.ENSP00000264414 |
PTM databases
iPTMneti | Q13618 |
PhosphoSitePlusi | Q13618 |
Genetic variation databases
BioMutai | CUL3 |
DMDMi | 12643396 |
Proteomic databases
EPDi | Q13618 |
jPOSTi | Q13618 |
MassIVEi | Q13618 |
MaxQBi | Q13618 |
PaxDbi | Q13618 |
PeptideAtlasi | Q13618 |
PRIDEi | Q13618 |
ProteomicsDBi | 59606 [Q13618-1] 59607 [Q13618-2] 59608 [Q13618-3] |
Protocols and materials databases
Antibodypediai | 3632, 398 antibodies from 40 providers |
DNASUi | 8452 |
Genome annotation databases
Ensembli | ENST00000264414.9; ENSP00000264414.4; ENSG00000036257.14 ENST00000344951.8; ENSP00000343601.4; ENSG00000036257.14 [Q13618-3] ENST00000409096.5; ENSP00000387200.1; ENSG00000036257.14 [Q13618-2] ENST00000409777.5; ENSP00000386525.1; ENSG00000036257.14 [Q13618-2] |
GeneIDi | 8452 |
KEGGi | hsa:8452 |
MANE-Selecti | ENST00000264414.9; ENSP00000264414.4; NM_003590.5; NP_003581.1 |
UCSCi | uc002vny.4, human [Q13618-1] |
Organism-specific databases
CTDi | 8452 |
DisGeNETi | 8452 |
GeneCardsi | CUL3 |
GeneReviewsi | CUL3 |
HGNCi | HGNC:2553, CUL3 |
HPAi | ENSG00000036257, Tissue enhanced (testis) |
MalaCardsi | CUL3 |
MIMi | 603136, gene 614496, phenotype 619239, phenotype |
neXtProti | NX_Q13618 |
OpenTargetsi | ENSG00000036257 |
Orphaneti | 300530, Pseudohypoaldosteronism type 2E |
PharmGKBi | PA27049 |
VEuPathDBi | HostDB:ENSG00000036257 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2166, Eukaryota |
GeneTreei | ENSGT00940000155066 |
HOGENOMi | CLU_004747_7_1_1 |
InParanoidi | Q13618 |
OMAi | MFKDMTI |
OrthoDBi | 1040292at2759 |
PhylomeDBi | Q13618 |
TreeFami | TF105858 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
PathwayCommonsi | Q13618 |
Reactomei | R-HSA-4641258, Degradation of DVL R-HSA-5632684, Hedgehog 'on' state R-HSA-5658442, Regulation of RAS by GAPs R-HSA-8932339, ROS sensing by NFE2L2 R-HSA-8951664, Neddylation R-HSA-9013418, RHOBTB2 GTPase cycle R-HSA-9013422, RHOBTB1 GTPase cycle R-HSA-9679191, Potential therapeutics for SARS R-HSA-9706019, RHOBTB3 ATPase cycle R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
SignaLinki | Q13618 |
SIGNORi | Q13618 |
Miscellaneous databases
BioGRID-ORCSi | 8452, 518 hits in 1155 CRISPR screens |
ChiTaRSi | CUL3, human |
GeneWikii | CUL3 |
GenomeRNAii | 8452 |
Pharosi | Q13618, Tbio |
PROi | PR:Q13618 |
RNActi | Q13618, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000036257, Expressed in sperm and 241 other tissues |
ExpressionAtlasi | Q13618, baseline and differential |
Genevisiblei | Q13618, HS |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR045093, Cullin IPR016157, Cullin_CS IPR016158, Cullin_homology IPR036317, Cullin_homology_sf IPR001373, Cullin_N IPR019559, Cullin_neddylation_domain IPR016159, Cullin_repeat-like_dom_sf IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR11932, PTHR11932, 1 hit |
Pfami | View protein in Pfam PF00888, Cullin, 1 hit PF10557, Cullin_Nedd8, 1 hit |
SMARTi | View protein in SMART SM00182, CULLIN, 1 hit SM00884, Cullin_Nedd8, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF74788, SSF74788, 1 hit SSF75632, SSF75632, 1 hit |
PROSITEi | View protein in PROSITE PS01256, CULLIN_1, 1 hit PS50069, CULLIN_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CUL3_HUMAN | |
Accessioni | Q13618Primary (citable) accession number: Q13618 Secondary accession number(s): A8K536 Q9UET7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | January 24, 2001 | |
Last modified: | May 25, 2022 | |
This is version 215 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families