UniProtKB - Q13568 (IRF5_HUMAN)
Interferon regulatory factor 5
IRF5
Functioni
Transcription factor that plays a critical role in innate immunity by activating expression of type I interferon (IFN) IFNA and INFB and inflammatory cytokines downstream of endolysosomal toll-like receptors TLR7, TLR8 and TLR9 (PubMed:11303025, PubMed:15695821, PubMed:22412986, PubMed:25326418, PubMed:32433612).
Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters (By similarity).
Can efficiently activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction downstream of the TLR-activated, MyD88-dependent pathway (By similarity).
Key transcription factor regulating the IFN response during SARS-CoV-2 infection (PubMed:33440148).
By similarity6 PublicationsActivity regulationi
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 14 – 122 | IRF tryptophan pentad repeatPROSITE-ProRule annotationAdd BLAST | 109 |
GO - Molecular functioni
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: NTNU_SB
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: UniProtKB
- identical protein binding Source: IntAct
- protein kinase binding Source: UniProtKB
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- sequence-specific DNA binding Source: NTNU_SB
- sequence-specific double-stranded DNA binding Source: ARUK-UCL
GO - Biological processi
- cytokine-mediated signaling pathway Source: UniProtKB
- defense response to virus Source: UniProtKB-KW
- immune system process Source: GO_Central
- inflammatory response Source: UniProtKB-KW
- innate immune response Source: UniProtKB
- positive regulation of apoptotic process Source: BHF-UCL
- positive regulation of interferon-alpha production Source: BHF-UCL
- positive regulation of interferon-beta production Source: BHF-UCL
- positive regulation of interleukin-12 production Source: BHF-UCL
- positive regulation of transcription by RNA polymerase II Source: UniProtKB
- positive regulation of type I interferon production Source: UniProtKB
- regulation of transcription by RNA polymerase II Source: GO_Central
- response to muramyl dipeptide Source: BHF-UCL
- response to peptidoglycan Source: BHF-UCL
Keywordsi
Molecular function | DNA-binding |
Biological process | Antiviral defense, Immunity, Inflammatory response, Innate immunity, Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | Q13568 |
Reactomei | R-HSA-877300, Interferon gamma signaling R-HSA-909733, Interferon alpha/beta signaling |
SignaLinki | Q13568 |
SIGNORi | Q13568 |
Names & Taxonomyi
Protein namesi | Recommended name: Interferon regulatory factor 51 PublicationShort name: IRF-51 Publication |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:6120, IRF5 |
MIMi | 607218, gene |
neXtProti | NX_Q13568 |
VEuPathDBi | HostDB:ENSG00000128604 |
Pathology & Biotechi
Involvement in diseasei
Inflammatory bowel disease 14 (IBD14)1 Publication
Systemic lupus erythematosus 10 (SLEB10)1 Publication
Rheumatoid arthritis (RA)1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 446 | S → A: Abolished nuclear translocation. 1 Publication | 1 |
Keywords - Diseasei
Systemic lupus erythematosusOrganism-specific databases
DisGeNETi | 3663 |
MalaCardsi | IRF5 |
MIMi | 180300, phenotype 612245, phenotype 612251, phenotype |
OpenTargetsi | ENSG00000128604 |
Orphaneti | 220393, Diffuse cutaneous systemic sclerosis 220402, Limited cutaneous systemic sclerosis 206, NON RARE IN EUROPE: Crohn disease 771, NON RARE IN EUROPE: Ulcerative colitis 186, Primary biliary cholangitis 536, Systemic lupus erythematosus |
PharmGKBi | PA29919 |
Miscellaneous databases
Pharosi | Q13568, Tbio |
Genetic variation databases
BioMutai | IRF5 |
DMDMi | 20178305 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000154558 | 1 – 498 | Interferon regulatory factor 5Add BLAST | 498 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 10 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 158 | Phosphoserine; by TBK11 Publication | 1 | |
Modified residuei | 293 | Phosphoserine; by TBK11 Publication | 1 | |
Modified residuei | 301 | Phosphoserine1 Publication | 1 | |
Cross-linki | 411 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication | ||
Cross-linki | 412 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication | ||
Modified residuei | 431 | PhosphoserineBy similarity | 1 | |
Modified residuei | 435 | Phosphoserine2 Publications | 1 | |
Modified residuei | 437 | Phosphoserine1 Publication | 1 | |
Modified residuei | 440 | Phosphoserine1 Publication | 1 | |
Modified residuei | 446 | Phosphoserine; by IKKB3 Publications | 1 |
Post-translational modificationi
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q13568 |
jPOSTi | Q13568 |
MassIVEi | Q13568 |
MaxQBi | Q13568 |
PaxDbi | Q13568 |
PeptideAtlasi | Q13568 |
PRIDEi | Q13568 |
ProteomicsDBi | 18776 59569 [Q13568-1] 59570 [Q13568-2] 59571 [Q13568-3] 59572 [Q13568-4] 61205 |
PTM databases
iPTMneti | Q13568 |
PhosphoSitePlusi | Q13568 |
Expressioni
Gene expression databases
Bgeei | ENSG00000128604, Expressed in monocyte and 185 other tissues |
ExpressionAtlasi | Q13568, baseline and differential |
Genevisiblei | Q13568, HS |
Organism-specific databases
HPAi | ENSG00000128604, Low tissue specificity |
Interactioni
Subunit structurei
Binary interactionsi
Q13568
With | #Exp. | IntAct |
---|---|---|
CREBBP [Q92793] | 3 | EBI-3931258,EBI-81215 |
itself | 3 | EBI-3931258,EBI-3931258 |
MAVS [Q7Z434] | 2 | EBI-3931258,EBI-995373 |
RELA [Q04206] | 4 | EBI-3931258,EBI-73886 |
SGTA [O43765] | 3 | EBI-3931258,EBI-347996 |
GO - Molecular functioni
- identical protein binding Source: IntAct
- protein kinase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 109871, 39 interactors |
DIPi | DIP-46348N |
IntActi | Q13568, 25 interactors |
STRINGi | 9606.ENSP00000349770 |
Miscellaneous databases
RNActi | Q13568, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q13568 |
SMRi | Q13568 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q13568 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 121 – 207 | DisorderedSequence analysisAdd BLAST | 87 | |
Regioni | 478 – 498 | DisorderedSequence analysisAdd BLAST | 21 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 12 – 18 | Nuclear localization signal1 Publication | 7 | |
Motifi | 150 – 160 | Nuclear export signal1 PublicationAdd BLAST | 11 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 169 – 206 | Pro residuesSequence analysisAdd BLAST | 38 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG502QSKM, Eukaryota |
GeneTreei | ENSGT00940000159926 |
HOGENOMi | CLU_031544_0_1_1 |
InParanoidi | Q13568 |
OMAi | VPWHHAT |
PhylomeDBi | Q13568 |
TreeFami | TF328512 |
Family and domain databases
CDDi | cd00103, IRF, 1 hit |
DisProti | DP01907 |
Gene3Di | 1.10.10.10, 1 hit 2.60.200.10, 1 hit |
IDEALi | IID00471 |
InterProi | View protein in InterPro IPR019817, Interferon_reg_fac_CS IPR001346, Interferon_reg_fact_DNA-bd_dom IPR019471, Interferon_reg_factor-3 IPR029838, IRF5 IPR017855, SMAD-like_dom_sf IPR008984, SMAD_FHA_dom_sf IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR11949, PTHR11949, 1 hit PTHR11949:SF10, PTHR11949:SF10, 1 hit |
Pfami | View protein in Pfam PF00605, IRF, 1 hit PF10401, IRF-3, 1 hit |
PRINTSi | PR00267, INTFRNREGFCT |
SMARTi | View protein in SMART SM00348, IRF, 1 hit SM01243, IRF-3, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF49879, SSF49879, 1 hit |
PROSITEi | View protein in PROSITE PS00601, IRF_1, 1 hit PS51507, IRF_2, 1 hit |
s (6+)i Sequence
Sequence statusi: Complete.
This entry describes 6 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 6 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MNQSIPVAPT PPRRVRLKPW LVAQVNSCQY PGLQWVNGEK KLFCIPWRHA
60 70 80 90 100
TRHGPSQDGD NTIFKAWAKE TGKYTEGVDE ADPAKWKANL RCALNKSRDF
110 120 130 140 150
RLIYDGPRDM PPQPYKIYEV CSNGPAPTDS QPPEDYSFGA GEEEEEEEEL
160 170 180 190 200
QRMLPSLSLT EDVKWPPTLQ PPTLRPPTLQ PPTLQPPVVL GPPAPDPSPL
210 220 230 240 250
APPPGNPAGF RELLSEVLEP GPLPASLPPA GEQLLPDLLI SPHMLPLTDL
260 270 280 290 300
EIKFQYRGRP PRALTISNPH GCRLFYSQLE ATQEQVELFG PISLEQVRFP
310 320 330 340 350
SPEDIPSDKQ RFYTNQLLDV LDRGLILQLQ GQDLYAIRLC QCKVFWSGPC
360 370 380 390 400
ASAHDSCPNP IQREVKTKLF SLEHFLNELI LFQKGQTNTP PPFEIFFCFG
410 420 430 440 450
EEWPDRKPRE KKLITVQVVP VAARLLLEMF SGELSWSADS IRLQISNPDL
460 470 480 490
KDRMVEQFKE LHHIWQSQQR LQPVAQAPPG AGLGVGQGPW PMHPAGMQ
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC9J7M2 | C9J7M2_HUMAN | Interferon regulatory factor 5 | IRF5 | 141 | Annotation score: | ||
A0A087WWA0 | A0A087WWA0_HUMAN | Interferon regulatory factor 5 | IRF5 | 157 | Annotation score: | ||
C9JYP7 | C9JYP7_HUMAN | Interferon regulatory factor 5 | IRF5 | 106 | Annotation score: | ||
C9JB67 | C9JB67_HUMAN | Interferon regulatory factor 5 | IRF5 | 59 | Annotation score: | ||
F8WDH6 | F8WDH6_HUMAN | Interferon regulatory factor 5 | IRF5 | 104 | Annotation score: | ||
A0A0G2UM11 | A0A0G2UM11_HUMAN | Interferon regulatory factor 5 | IRF5 | 47 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 253 | K → KK in AAU12880 (PubMed:15805103).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_053330 | 120 – 147 | VCSNG…EEEEE → TPSPLRITLLVQERRRKKRK SCRGCCQA in isoform 6. 1 PublicationAdd BLAST | 28 | |
Alternative sequenceiVSP_053331 | 148 – 498 | Missing in isoform 6. 1 PublicationAdd BLAST | 351 | |
Alternative sequenceiVSP_041375 | 160 | T → TDAVQSGPHMTPYSLLK in isoform 2. 2 Publications | 1 | |
Alternative sequenceiVSP_044822 | 161 – 247 | EDVKW…HMLPL → V in isoform 5. 1 PublicationAdd BLAST | 87 | |
Alternative sequenceiVSP_043924 | 161 – 175 | EDVKW…PPTLR → DAVQSGPHMTPYSLLKEDVK W in isoform 3. 2 PublicationsAdd BLAST | 15 | |
Alternative sequenceiVSP_043925 | 166 – 175 | Missing in isoform 4. 3 Publications | 10 |
Sequence databases
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3DSH | X-ray | 2.00 | A | 232-477 | [»] | |
AlphaFoldDBi | Q13568 | |||||
SMRi | Q13568 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 109871, 39 interactors |
DIPi | DIP-46348N |
IntActi | Q13568, 25 interactors |
STRINGi | 9606.ENSP00000349770 |
PTM databases
iPTMneti | Q13568 |
PhosphoSitePlusi | Q13568 |
Genetic variation databases
BioMutai | IRF5 |
DMDMi | 20178305 |
Proteomic databases
EPDi | Q13568 |
jPOSTi | Q13568 |
MassIVEi | Q13568 |
MaxQBi | Q13568 |
PaxDbi | Q13568 |
PeptideAtlasi | Q13568 |
PRIDEi | Q13568 |
ProteomicsDBi | 18776 59569 [Q13568-1] 59570 [Q13568-2] 59571 [Q13568-3] 59572 [Q13568-4] 61205 |
Protocols and materials databases
ABCDi | Q13568, 1 sequenced antibody |
Antibodypediai | 17820, 622 antibodies from 45 providers |
DNASUi | 3663 |
Genome annotation databases
Organism-specific databases
CTDi | 3663 |
DisGeNETi | 3663 |
GeneCardsi | IRF5 |
HGNCi | HGNC:6120, IRF5 |
HPAi | ENSG00000128604, Low tissue specificity |
MalaCardsi | IRF5 |
MIMi | 180300, phenotype 607218, gene 612245, phenotype 612251, phenotype |
neXtProti | NX_Q13568 |
OpenTargetsi | ENSG00000128604 |
Orphaneti | 220393, Diffuse cutaneous systemic sclerosis 220402, Limited cutaneous systemic sclerosis 206, NON RARE IN EUROPE: Crohn disease 771, NON RARE IN EUROPE: Ulcerative colitis 186, Primary biliary cholangitis 536, Systemic lupus erythematosus |
PharmGKBi | PA29919 |
VEuPathDBi | HostDB:ENSG00000128604 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QSKM, Eukaryota |
GeneTreei | ENSGT00940000159926 |
HOGENOMi | CLU_031544_0_1_1 |
InParanoidi | Q13568 |
OMAi | VPWHHAT |
PhylomeDBi | Q13568 |
TreeFami | TF328512 |
Enzyme and pathway databases
PathwayCommonsi | Q13568 |
Reactomei | R-HSA-877300, Interferon gamma signaling R-HSA-909733, Interferon alpha/beta signaling |
SignaLinki | Q13568 |
SIGNORi | Q13568 |
Miscellaneous databases
BioGRID-ORCSi | 3663, 15 hits in 1097 CRISPR screens |
EvolutionaryTracei | Q13568 |
GeneWikii | IRF5 |
GenomeRNAii | 3663 |
Pharosi | Q13568, Tbio |
PROi | PR:Q13568 |
RNActi | Q13568, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000128604, Expressed in monocyte and 185 other tissues |
ExpressionAtlasi | Q13568, baseline and differential |
Genevisiblei | Q13568, HS |
Family and domain databases
CDDi | cd00103, IRF, 1 hit |
DisProti | DP01907 |
Gene3Di | 1.10.10.10, 1 hit 2.60.200.10, 1 hit |
IDEALi | IID00471 |
InterProi | View protein in InterPro IPR019817, Interferon_reg_fac_CS IPR001346, Interferon_reg_fact_DNA-bd_dom IPR019471, Interferon_reg_factor-3 IPR029838, IRF5 IPR017855, SMAD-like_dom_sf IPR008984, SMAD_FHA_dom_sf IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR11949, PTHR11949, 1 hit PTHR11949:SF10, PTHR11949:SF10, 1 hit |
Pfami | View protein in Pfam PF00605, IRF, 1 hit PF10401, IRF-3, 1 hit |
PRINTSi | PR00267, INTFRNREGFCT |
SMARTi | View protein in SMART SM00348, IRF, 1 hit SM01243, IRF-3, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF49879, SSF49879, 1 hit |
PROSITEi | View protein in PROSITE PS00601, IRF_1, 1 hit PS51507, IRF_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | IRF5_HUMAN | |
Accessioni | Q13568Primary (citable) accession number: Q13568 Secondary accession number(s): A4D1J8 Q9BQF0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | April 16, 2002 | |
Last modified: | May 25, 2022 | |
This is version 186 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 7
Human chromosome 7: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families