UniProtKB - Q13561 (DCTN2_HUMAN)
Protein
Dynactin subunit 2
Gene
DCTN2
Organism
Homo sapiens (Human)
Status
Functioni
Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development.
GO - Molecular functioni
- identical protein binding Source: IntAct
- motor activity Source: UniProtKB-KW
- protein kinase binding Source: ARUK-UCL
- spectrin binding Source: MGI
GO - Biological processi
- antigen processing and presentation of exogenous peptide antigen via MHC class II Source: Reactome
- ciliary basal body-plasma membrane docking Source: Reactome
- endoplasmic reticulum to Golgi vesicle-mediated transport Source: Reactome
- G2/M transition of mitotic cell cycle Source: Reactome
- melanosome transport Source: Ensembl
- mitotic metaphase plate congression Source: UniProtKB
- mitotic spindle organization Source: UniProtKB
- protein localization to centrosome Source: SYSCILIA_CCNET
- regulation of G2/M transition of mitotic cell cycle Source: Reactome
Enzyme and pathway databases
PathwayCommonsi | Q13561 |
Reactomei | R-HSA-2132295, MHC class II antigen presentation R-HSA-2565942, Regulation of PLK1 Activity at G2/M Transition R-HSA-3371497, HSP90 chaperone cycle for steroid hormone receptors (SHR) R-HSA-380259, Loss of Nlp from mitotic centrosomes R-HSA-380270, Recruitment of mitotic centrosome proteins and complexes R-HSA-380284, Loss of proteins required for interphase microtubule organization from the centrosome R-HSA-380320, Recruitment of NuMA to mitotic centrosomes R-HSA-5620912, Anchoring of the basal body to the plasma membrane R-HSA-6807878, COPI-mediated anterograde transport R-HSA-6811436, COPI-independent Golgi-to-ER retrograde traffic R-HSA-8854518, AURKA Activation by TPX2 |
SignaLinki | Q13561 |
Names & Taxonomyi
Protein namesi | Recommended name: Dynactin subunit 2Alternative name(s): 50 kDa dynein-associated polypeptide Dynactin complex 50 kDa subunit Short name: DCTN-50 p50 dynamitin |
Gene namesi | Name:DCTN2 Synonyms:DCTN50 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:2712, DCTN2 |
MIMi | 607376, gene |
neXtProti | NX_Q13561 |
VEuPathDBi | HostDB:ENSG00000175203.15 |
Subcellular locationi
Cytoskeleton
- centrosome 2 Publications
Other locations
- Membrane 1 Publication; Peripheral membrane protein 1 Publication
Cytoskeleton
- centrosome Source: UniProtKB
- dynactin complex Source: UniProtKB
- dynein complex Source: UniProtKB-KW
- microtubule Source: UniProtKB-KW
Cytosol
- cytosol Source: HPA
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Other locations
- cytoplasm Source: GO_Central
- growth cone Source: Ensembl
- kinetochore Source: UniProtKB
- membrane Source: UniProtKB-SubCell
- vesicle Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, Dynein, Membrane, MicrotubulePathology & Biotechi
Organism-specific databases
DisGeNETi | 10540 |
GeneReviewsi | DCTN2 |
MalaCardsi | DCTN2 |
OpenTargetsi | ENSG00000175203 |
PharmGKBi | PA27181 |
Miscellaneous databases
Pharosi | Q13561, Tbio |
Genetic variation databases
BioMutai | DCTN2 |
DMDMi | 22096346 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources2 Publications | |||
ChainiPRO_0000079821 | 2 – 401 | Dynactin subunit 2Add BLAST | 400 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources1 Publication | 1 | |
Modified residuei | 6 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 83 | PhosphoserineCombined sources | 1 | |
Modified residuei | 86 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 134 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 198 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 320 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q13561 |
jPOSTi | Q13561 |
MassIVEi | Q13561 |
MaxQBi | Q13561 |
PaxDbi | Q13561 |
PeptideAtlasi | Q13561 |
PRIDEi | Q13561 |
ProteomicsDBi | 59558 [Q13561-1] 59559 [Q13561-2] 59560 [Q13561-3] |
2D gel databases
REPRODUCTION-2DPAGEi | IPI00220503 |
PTM databases
iPTMneti | Q13561 |
MetOSitei | Q13561 |
PhosphoSitePlusi | Q13561 |
Expressioni
Gene expression databases
Bgeei | ENSG00000175203, Expressed in frontal cortex and 245 other tissues |
ExpressionAtlasi | Q13561, baseline and differential |
Genevisiblei | Q13561, HS |
Organism-specific databases
HPAi | ENSG00000175203, Low tissue specificity |
Interactioni
Subunit structurei
Binary interactionsi
Hide detailsQ13561
GO - Molecular functioni
- identical protein binding Source: IntAct
- protein kinase binding Source: ARUK-UCL
- spectrin binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 115794, 186 interactors |
DIPi | DIP-37545N |
IntActi | Q13561, 113 interactors |
MINTi | Q13561 |
STRINGi | 9606.ENSP00000408910 |
Miscellaneous databases
RNActi | Q13561, protein |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 99 – 132 | Sequence analysisAdd BLAST | 34 | |
Coiled coili | 214 – 244 | Sequence analysisAdd BLAST | 31 | |
Coiled coili | 379 – 399 | Sequence analysisAdd BLAST | 21 |
Sequence similaritiesi
Belongs to the dynactin subunit 2 family.Curated
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG3958, Eukaryota |
GeneTreei | ENSGT00390000003427 |
InParanoidi | Q13561 |
OMAi | HSVQETF |
OrthoDBi | 951183at2759 |
PhylomeDBi | Q13561 |
TreeFami | TF105247 |
Family and domain databases
InterProi | View protein in InterPro IPR028133, Dynamitin |
PANTHERi | PTHR15346, PTHR15346, 1 hit |
Pfami | View protein in Pfam PF04912, Dynamitin, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q13561-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MADPKYADLP GIARNEPDVY ETSDLPEDDQ AEFDAEELTS TSVEHIIVNP
60 70 80 90 100
NAAYDKFKDK RVGTKGLDFS DRIGKTKRTG YESGEYEMLG EGLGVKETPQ
110 120 130 140 150
QKYQRLLHEV QELTTEVEKI KTTVKESATE EKLTPVLLAK QLAALKQQLV
160 170 180 190 200
ASHLEKLLGP DAAINLTDPD GALAKRLLLQ LEATKNSKGG SGGKTTGTPP
210 220 230 240 250
DSSLVTYELH SRPEQDKFSQ AAKVAELEKR LTELETAVRC DQDAQNPLSA
260 270 280 290 300
GLQGACLMET VELLQAKVSA LDLAVLDQVE ARLQSVLGKV NEIAKHKASV
310 320 330 340 350
EDADTQSKVH QLYETIQRWS PIASTLPELV QRLVTIKQLH EQAMQFGQLL
360 370 380 390 400
THLDTTQQMI ANSLKDNTTL LTQVQTTMRE NLATVEGNFA SIDERMKKLG
K
Computationally mapped potential isoform sequencesi
There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF8VW18 | F8VW18_HUMAN | Dynactin subunit 2 | DCTN2 | 278 | Annotation score: | ||
F8VRV7 | F8VRV7_HUMAN | Dynactin subunit 2 | DCTN2 | 152 | Annotation score: | ||
F8W1I6 | F8W1I6_HUMAN | Dynactin subunit 2 | DCTN2 | 315 | Annotation score: | ||
F8VX93 | F8VX93_HUMAN | Dynactin subunit 2 | DCTN2 | 135 | Annotation score: | ||
H0YI98 | H0YI98_HUMAN | Dynactin subunit 2 | DCTN2 | 268 | Annotation score: | ||
H0YHL1 | H0YHL1_HUMAN | Dynactin subunit 2 | DCTN2 | 128 | Annotation score: | ||
F8W0U6 | F8W0U6_HUMAN | Dynactin subunit 2 | DCTN2 | 45 | Annotation score: |
Sequence cautioni
The sequence AAO34395 differs from that shown. Reason: Frameshift.Curated
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_040485 | 35 | A → AFAQEL in isoform 2. 2 Publications | 1 | |
Alternative sequenceiVSP_040486 | 35 | A → AEL in isoform 3. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U50733 mRNA Translation: AAC50423.1 AK314705 mRNA Translation: BAG37252.1 AC022366 Genomic DNA No translation available. AC022506 Genomic DNA No translation available. BC000718 mRNA Translation: AAH00718.1 BC009468 mRNA Translation: AAH09468.1 BC014083 mRNA Translation: AAH14083.1 AY189155 mRNA Translation: AAO34395.1 Frameshift. |
CCDSi | CCDS44930.1 [Q13561-2] CCDS58245.1 [Q13561-1] CCDS73489.1 [Q13561-3] |
RefSeqi | NP_001248341.1, NM_001261412.1 [Q13561-3] NP_001248342.1, NM_001261413.1 [Q13561-1] NP_006391.1, NM_006400.4 [Q13561-2] |
Genome annotation databases
Ensembli | ENST00000434715; ENSP00000408910; ENSG00000175203 [Q13561-2] ENST00000543672; ENSP00000439376; ENSG00000175203 [Q13561-3] ENST00000548249; ENSP00000447824; ENSG00000175203 [Q13561-1] |
GeneIDi | 10540 |
KEGGi | hsa:10540 |
UCSCi | uc001som.3, human [Q13561-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U50733 mRNA Translation: AAC50423.1 AK314705 mRNA Translation: BAG37252.1 AC022366 Genomic DNA No translation available. AC022506 Genomic DNA No translation available. BC000718 mRNA Translation: AAH00718.1 BC009468 mRNA Translation: AAH09468.1 BC014083 mRNA Translation: AAH14083.1 AY189155 mRNA Translation: AAO34395.1 Frameshift. |
CCDSi | CCDS44930.1 [Q13561-2] CCDS58245.1 [Q13561-1] CCDS73489.1 [Q13561-3] |
RefSeqi | NP_001248341.1, NM_001261412.1 [Q13561-3] NP_001248342.1, NM_001261413.1 [Q13561-1] NP_006391.1, NM_006400.4 [Q13561-2] |
3D structure databases
SMRi | Q13561 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 115794, 186 interactors |
DIPi | DIP-37545N |
IntActi | Q13561, 113 interactors |
MINTi | Q13561 |
STRINGi | 9606.ENSP00000408910 |
PTM databases
iPTMneti | Q13561 |
MetOSitei | Q13561 |
PhosphoSitePlusi | Q13561 |
Genetic variation databases
BioMutai | DCTN2 |
DMDMi | 22096346 |
2D gel databases
REPRODUCTION-2DPAGEi | IPI00220503 |
Proteomic databases
EPDi | Q13561 |
jPOSTi | Q13561 |
MassIVEi | Q13561 |
MaxQBi | Q13561 |
PaxDbi | Q13561 |
PeptideAtlasi | Q13561 |
PRIDEi | Q13561 |
ProteomicsDBi | 59558 [Q13561-1] 59559 [Q13561-2] 59560 [Q13561-3] |
Protocols and materials databases
Antibodypediai | 28711, 345 antibodies |
DNASUi | 10540 |
Genome annotation databases
Ensembli | ENST00000434715; ENSP00000408910; ENSG00000175203 [Q13561-2] ENST00000543672; ENSP00000439376; ENSG00000175203 [Q13561-3] ENST00000548249; ENSP00000447824; ENSG00000175203 [Q13561-1] |
GeneIDi | 10540 |
KEGGi | hsa:10540 |
UCSCi | uc001som.3, human [Q13561-1] |
Organism-specific databases
CTDi | 10540 |
DisGeNETi | 10540 |
GeneCardsi | DCTN2 |
GeneReviewsi | DCTN2 |
HGNCi | HGNC:2712, DCTN2 |
HPAi | ENSG00000175203, Low tissue specificity |
MalaCardsi | DCTN2 |
MIMi | 607376, gene |
neXtProti | NX_Q13561 |
OpenTargetsi | ENSG00000175203 |
PharmGKBi | PA27181 |
VEuPathDBi | HostDB:ENSG00000175203.15 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3958, Eukaryota |
GeneTreei | ENSGT00390000003427 |
InParanoidi | Q13561 |
OMAi | HSVQETF |
OrthoDBi | 951183at2759 |
PhylomeDBi | Q13561 |
TreeFami | TF105247 |
Enzyme and pathway databases
PathwayCommonsi | Q13561 |
Reactomei | R-HSA-2132295, MHC class II antigen presentation R-HSA-2565942, Regulation of PLK1 Activity at G2/M Transition R-HSA-3371497, HSP90 chaperone cycle for steroid hormone receptors (SHR) R-HSA-380259, Loss of Nlp from mitotic centrosomes R-HSA-380270, Recruitment of mitotic centrosome proteins and complexes R-HSA-380284, Loss of proteins required for interphase microtubule organization from the centrosome R-HSA-380320, Recruitment of NuMA to mitotic centrosomes R-HSA-5620912, Anchoring of the basal body to the plasma membrane R-HSA-6807878, COPI-mediated anterograde transport R-HSA-6811436, COPI-independent Golgi-to-ER retrograde traffic R-HSA-8854518, AURKA Activation by TPX2 |
SignaLinki | Q13561 |
Miscellaneous databases
BioGRID-ORCSi | 10540, 630 hits in 882 CRISPR screens |
ChiTaRSi | DCTN2, human |
GeneWikii | DCTN2 |
GenomeRNAii | 10540 |
Pharosi | Q13561, Tbio |
PROi | PR:Q13561 |
RNActi | Q13561, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000175203, Expressed in frontal cortex and 245 other tissues |
ExpressionAtlasi | Q13561, baseline and differential |
Genevisiblei | Q13561, HS |
Family and domain databases
InterProi | View protein in InterPro IPR028133, Dynamitin |
PANTHERi | PTHR15346, PTHR15346, 1 hit |
Pfami | View protein in Pfam PF04912, Dynamitin, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DCTN2_HUMAN | |
Accessioni | Q13561Primary (citable) accession number: Q13561 Secondary accession number(s): B2RBK5, Q86YN2, Q9BW17 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 10, 2021 | |
This is version 204 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families