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Protein

Dynactin subunit 2

Gene

DCTN2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development.

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • motor activity Source: UniProtKB-KW
  • protein kinase binding Source: ARUK-UCL
  • spectrin binding Source: MGI

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-HSA-2132295 MHC class II antigen presentation
R-HSA-2565942 Regulation of PLK1 Activity at G2/M Transition
R-HSA-3371497 HSP90 chaperone cycle for steroid hormone receptors (SHR)
R-HSA-380259 Loss of Nlp from mitotic centrosomes
R-HSA-380270 Recruitment of mitotic centrosome proteins and complexes
R-HSA-380284 Loss of proteins required for interphase microtubule organization from the centrosome
R-HSA-380320 Recruitment of NuMA to mitotic centrosomes
R-HSA-5620912 Anchoring of the basal body to the plasma membrane
R-HSA-6807878 COPI-mediated anterograde transport
R-HSA-6811436 COPI-independent Golgi-to-ER retrograde traffic
R-HSA-8854518 AURKA Activation by TPX2
SignaLinkiQ13561

Names & Taxonomyi

Protein namesi
Recommended name:
Dynactin subunit 2
Alternative name(s):
50 kDa dynein-associated polypeptide
Dynactin complex 50 kDa subunit
Short name:
DCTN-50
p50 dynamitin
Gene namesi
Name:DCTN2
Synonyms:DCTN50
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000175203.15
HGNCiHGNC:2712 DCTN2
MIMi607376 gene
neXtProtiNX_Q13561

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Dynein, Membrane, Microtubule

Pathology & Biotechi

Organism-specific databases

DisGeNETi10540
OpenTargetsiENSG00000175203
PharmGKBiPA27181

Polymorphism and mutation databases

BioMutaiDCTN2
DMDMi22096346

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources2 Publications
ChainiPRO_00000798212 – 401Dynactin subunit 2Add BLAST400

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1 Publication1
Modified residuei6PhosphotyrosineCombined sources1
Modified residuei83PhosphoserineCombined sources1
Modified residuei86PhosphotyrosineBy similarity1
Modified residuei134PhosphothreonineCombined sources1
Modified residuei198PhosphothreonineCombined sources1
Modified residuei320PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ13561
MaxQBiQ13561
PaxDbiQ13561
PeptideAtlasiQ13561
PRIDEiQ13561
ProteomicsDBi59558
59559 [Q13561-2]
59560 [Q13561-3]

2D gel databases

REPRODUCTION-2DPAGEiIPI00220503

PTM databases

iPTMnetiQ13561
PhosphoSitePlusiQ13561

Miscellaneous databases

PMAP-CutDBiQ13561

Expressioni

Gene expression databases

BgeeiENSG00000175203
CleanExiHS_DCTN2
ExpressionAtlasiQ13561 baseline and differential
GenevisibleiQ13561 HS

Organism-specific databases

HPAiHPA039715
HPA040040

Interactioni

Subunit structurei

Subunit of dynactin, a multiprotein complex associated with dynein. Interacts with BICD2 and CEP135 (By similarity). Interacts with DYNAP (PubMed:20978158). Interacts with ECPAS (PubMed:20682791). Interacts with MAPRE1 (PubMed:10226031).By similarity3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • protein kinase binding Source: ARUK-UCL
  • spectrin binding Source: MGI

Protein-protein interaction databases

BioGridi115794, 123 interactors
CORUMiQ13561
DIPiDIP-37545N
IntActiQ13561, 84 interactors
MINTiQ13561
STRINGi9606.ENSP00000408910

Structurei

3D structure databases

ProteinModelPortaliQ13561
SMRiQ13561
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili99 – 132Sequence analysisAdd BLAST34
Coiled coili214 – 244Sequence analysisAdd BLAST31
Coiled coili379 – 399Sequence analysisAdd BLAST21

Sequence similaritiesi

Belongs to the dynactin subunit 2 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3958 Eukaryota
ENOG410XSZK LUCA
GeneTreeiENSGT00390000003427
HOVERGENiHBG051323
InParanoidiQ13561
KOiK10424
OMAiSGEYEML
PhylomeDBiQ13561
TreeFamiTF105247

Family and domain databases

InterProiView protein in InterPro
IPR028133 Dynamitin
PANTHERiPTHR15346 PTHR15346, 1 hit
PfamiView protein in Pfam
PF04912 Dynamitin, 1 hit

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q13561-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADPKYADLP GIARNEPDVY ETSDLPEDDQ AEFDAEELTS TSVEHIIVNP
60 70 80 90 100
NAAYDKFKDK RVGTKGLDFS DRIGKTKRTG YESGEYEMLG EGLGVKETPQ
110 120 130 140 150
QKYQRLLHEV QELTTEVEKI KTTVKESATE EKLTPVLLAK QLAALKQQLV
160 170 180 190 200
ASHLEKLLGP DAAINLTDPD GALAKRLLLQ LEATKNSKGG SGGKTTGTPP
210 220 230 240 250
DSSLVTYELH SRPEQDKFSQ AAKVAELEKR LTELETAVRC DQDAQNPLSA
260 270 280 290 300
GLQGACLMET VELLQAKVSA LDLAVLDQVE ARLQSVLGKV NEIAKHKASV
310 320 330 340 350
EDADTQSKVH QLYETIQRWS PIASTLPELV QRLVTIKQLH EQAMQFGQLL
360 370 380 390 400
THLDTTQQMI ANSLKDNTTL LTQVQTTMRE NLATVEGNFA SIDERMKKLG

K
Length:401
Mass (Da):44,231
Last modified:January 23, 2007 - v4
Checksum:iC2FF01A9739337B2
GO
Isoform 2 (identifier: Q13561-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     35-35: A → AFAQEL

Show »
Length:406
Mass (Da):44,820
Checksum:i1166997A6D07479D
GO
Isoform 3 (identifier: Q13561-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     35-35: A → AEL

Show »
Length:403
Mass (Da):44,473
Checksum:i864FD68AF5433E22
GO

Sequence cautioni

The sequence AAO34395 differs from that shown. Reason: Frameshift at position 381.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04048535A → AFAQEL in isoform 2. 2 Publications1
Alternative sequenceiVSP_04048635A → AEL in isoform 3. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U50733 mRNA Translation: AAC50423.1
AK314705 mRNA Translation: BAG37252.1
AC022366 Genomic DNA No translation available.
AC022506 Genomic DNA No translation available.
BC000718 mRNA Translation: AAH00718.1
BC009468 mRNA Translation: AAH09468.1
BC014083 mRNA Translation: AAH14083.1
AY189155 mRNA Translation: AAO34395.1 Frameshift.
CCDSiCCDS44930.1 [Q13561-2]
CCDS58245.1 [Q13561-1]
CCDS73489.1 [Q13561-3]
RefSeqiNP_001248341.1, NM_001261412.1 [Q13561-3]
NP_001248342.1, NM_001261413.1 [Q13561-1]
NP_006391.1, NM_006400.4 [Q13561-2]
UniGeneiHs.289123

Genome annotation databases

EnsembliENST00000434715; ENSP00000408910; ENSG00000175203 [Q13561-2]
ENST00000543672; ENSP00000439376; ENSG00000175203 [Q13561-3]
ENST00000548249; ENSP00000447824; ENSG00000175203 [Q13561-1]
GeneIDi10540
KEGGihsa:10540
UCSCiuc001som.3 human [Q13561-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiDCTN2_HUMAN
AccessioniPrimary (citable) accession number: Q13561
Secondary accession number(s): B2RBK5, Q86YN2, Q9BW17
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: June 20, 2018
This is version 183 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

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