UniProtKB - Q13427 (PPIG_HUMAN)
Protein
Peptidyl-prolyl cis-trans isomerase G
Gene
PPIG
Organism
Homo sapiens (Human)
Status
Functioni
PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding (PubMed:20676357). May be implicated in the folding, transport, and assembly of proteins. May play an important role in the regulation of pre-mRNA splicing.1 Publication
Catalytic activityi
- EC:5.2.1.81 Publication
Activity regulationi
Inhibited by cyclosporin A (CsA).1 Publication
GO - Molecular functioni
- cyclosporin A binding Source: UniProtKB
- peptidyl-prolyl cis-trans isomerase activity Source: UniProtKB
- RNA binding Source: UniProtKB
GO - Biological processi
- protein folding Source: GO_Central
- protein peptidyl-prolyl isomerization Source: UniProtKB
- RNA splicing Source: ProtInc
Keywordsi
Molecular function | Isomerase, Rotamase |
Enzyme and pathway databases
BRENDAi | 5.2.1.8, 2681 |
PathwayCommonsi | Q13427 |
Names & Taxonomyi
Protein namesi | Recommended name: Peptidyl-prolyl cis-trans isomerase G (EC:5.2.1.81 Publication)Short name: PPIase G Short name: Peptidyl-prolyl isomerase G Alternative name(s): CASP10 Clk-associating RS-cyclophilin Short name: CARS-Cyp Short name: CARS-cyclophilin Short name: SR-cyclophilin Short name: SR-cyp Short name: SRcyp Cyclophilin G Rotamase G |
Gene namesi | Name:PPIG |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:14650, PPIG |
MIMi | 606093, gene |
neXtProti | NX_Q13427 |
VEuPathDBi | HostDB:ENSG00000138398.15 |
Subcellular locationi
Nucleus
- Nucleus matrix 1 Publication
- Nucleus speckle 1 Publication
Note: Colocalizes with RNA splicing factors at nuclear speckles.1 Publication
Cytosol
- cytosol Source: HPA
Nucleus
- nuclear matrix Source: UniProtKB-SubCell
- nuclear speck Source: HPA
- nucleoplasm Source: ProtInc
- nucleus Source: GO_Central
Other locations
- cytoplasm Source: GO_Central
- intracellular membrane-bounded organelle Source: GO_Central
Keywords - Cellular componenti
NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 9360 |
OpenTargetsi | ENSG00000138398 |
PharmGKBi | PA33585 |
Miscellaneous databases
Pharosi | Q13427, Tchem |
Chemistry databases
ChEMBLi | CHEMBL3707467 |
DrugBanki | DB00172, Proline |
Genetic variation databases
BioMutai | PPIG |
DMDMi | 229462749 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000064150 | 1 – 754 | Peptidyl-prolyl cis-trans isomerase GAdd BLAST | 754 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 254 | PhosphoserineCombined sources | 1 | |
Modified residuei | 256 | PhosphoserineCombined sources | 1 | |
Modified residuei | 257 | PhosphoserineCombined sources | 1 | |
Modified residuei | 259 | PhosphoserineCombined sources | 1 | |
Modified residuei | 290 | PhosphoserineCombined sources | 1 | |
Modified residuei | 315 | PhosphoserineCombined sources | 1 | |
Modified residuei | 356 | PhosphoserineCombined sources | 1 | |
Modified residuei | 358 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 386 | PhosphoserineCombined sources | 1 | |
Cross-linki | 392 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 397 | PhosphoserineCombined sources | 1 | |
Modified residuei | 413 | PhosphoserineCombined sources | 1 | |
Modified residuei | 415 | PhosphoserineCombined sources | 1 | |
Modified residuei | 687 | PhosphoserineCombined sources | 1 | |
Modified residuei | 690 | PhosphoserineCombined sources | 1 | |
Cross-linki | 693 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 696 | PhosphoserineCombined sources | 1 | |
Modified residuei | 744 | PhosphoserineCombined sources | 1 | |
Modified residuei | 745 | PhosphoserineCombined sources | 1 | |
Modified residuei | 748 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 753 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q13427 |
jPOSTi | Q13427 |
MassIVEi | Q13427 |
MaxQBi | Q13427 |
PaxDbi | Q13427 |
PeptideAtlasi | Q13427 |
PRIDEi | Q13427 |
ProteomicsDBi | 59415 [Q13427-1] 59416 [Q13427-2] |
PTM databases
iPTMneti | Q13427 |
PhosphoSitePlusi | Q13427 |
Expressioni
Tissue specificityi
Ubiquitous.1 Publication
Gene expression databases
Bgeei | ENSG00000138398, Expressed in sural nerve and 256 other tissues |
ExpressionAtlasi | Q13427, baseline and differential |
Genevisiblei | Q13427, HS |
Organism-specific databases
HPAi | ENSG00000138398, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with CLK1, PNN and with the phosphorylated C-terminal domain of RNA polymerase II.
2 PublicationsBinary interactionsi
Hide detailsQ13427
Protein-protein interaction databases
BioGRIDi | 114761, 64 interactors |
IntActi | Q13427, 50 interactors |
MINTi | Q13427 |
STRINGi | 9606.ENSP00000260970 |
Chemistry databases
BindingDBi | Q13427 |
Miscellaneous databases
RNActi | Q13427, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q13427 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q13427 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 11 – 176 | PPIase cyclophilin-typePROSITE-ProRule annotationAdd BLAST | 166 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 180 – 192 | Arg/Lys-rich (basic)Add BLAST | 13 | |
Compositional biasi | 193 – 225 | Asp/Glu/Ser-richAdd BLAST | 33 | |
Compositional biasi | 193 – 208 | Poly-SerAdd BLAST | 16 | |
Compositional biasi | 226 – 253 | Arg/Lys-rich (basic)Add BLAST | 28 | |
Compositional biasi | 540 – 639 | Arg/Ser-rich (RS domain)Add BLAST | 100 | |
Compositional biasi | 621 – 626 | Poly-Arg | 6 |
Domaini
The RS domain is required for the interaction with the phosphorylated C-terminal domain of RNA polymerase II.1 Publication
Phylogenomic databases
eggNOGi | KOG0546, Eukaryota |
GeneTreei | ENSGT00940000157954 |
HOGENOMi | CLU_012062_33_6_1 |
InParanoidi | Q13427 |
OMAi | VVQTMEN |
OrthoDBi | 1403619at2759 |
PhylomeDBi | Q13427 |
TreeFami | TF318563 |
Family and domain databases
Gene3Di | 2.40.100.10, 1 hit |
InterProi | View protein in InterPro IPR029000, Cyclophilin-like_dom_sf IPR020892, Cyclophilin-type_PPIase_CS IPR002130, Cyclophilin-type_PPIase_dom |
Pfami | View protein in Pfam PF00160, Pro_isomerase, 1 hit |
PRINTSi | PR00153, CSAPPISMRASE |
SUPFAMi | SSF50891, SSF50891, 1 hit |
PROSITEi | View protein in PROSITE PS00170, CSA_PPIASE_1, 1 hit PS50072, CSA_PPIASE_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q13427-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGIKVQRPRC FFDIAINNQP AGRVVFELFS DVCPKTCENF RCLCTGEKGT
60 70 80 90 100
GKSTQKPLHY KSCLFHRVVK DFMVQGGDFS EGNGRGGESI YGGFFEDESF
110 120 130 140 150
AVKHNKEFLL SMANRGKDTN GSQFFITTKP TPHLDGHHVV FGQVISGQEV
160 170 180 190 200
VREIENQKTD AASKPFAEVR ILSCGELIPK SKVKKEEKKR HKSSSSSSSS
210 220 230 240 250
SSDSDSSSDS QSSSDSSDSE SATEEKSKKR KKKHRKNSRK HKKEKKKRKK
260 270 280 290 300
SKKSASSESE AENLEAQPQS TVRPEEIPPI PENRFLMRKS PPKADEKERK
310 320 330 340 350
NREREREREC NPPNSQPASY QRRLLVTRSG RKIKGRGPRR YRTPSRSRSR
360 370 380 390 400
DRFRRSETPP HWRQEMQRAQ RMRVSSGERW IKGDKSELNE IKENQRSPVR
410 420 430 440 450
VKERKITDHR NVSESPNRKN EKEKKVKDHK SNSKERDIRR NSEKDDKYKN
460 470 480 490 500
KVKKRAKSKS RSKSKEKSKS KERDSKHNRN EEKRMRSRSK GRDHENVKEK
510 520 530 540 550
EKQSDSKGKD QERSRSKEKS KQLESKSNEH DHSKSKEKDR RAQSRSRECD
560 570 580 590 600
ITKGKHSYNS RTRERSRSRD RSRRVRSRTH DRDRSRSKEY HRYREQEYRR
610 620 630 640 650
RGRSRSRERR TPPGRSRSKD RRRRRRDSRS SEREESQSRN KDKYRNQESK
660 670 680 690 700
SSHRKENSES EKRMYSKSRD HNSSNNSREK KADRDQSPFS KIKQSSQDNE
710 720 730 740 750
LKSSMLKNKE DEKIRSSVEK ENQKSKGQEN DHVHEKNKKF DHESSPGTDE
DKSG
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC9JN15 | C9JN15_HUMAN | Peptidyl-prolyl cis-trans isomerase | PPIG | 246 | Annotation score: | ||
E9PG73 | E9PG73_HUMAN | Peptidyl-prolyl cis-trans isomerase... | PPIG | 739 | Annotation score: | ||
C9JM79 | C9JM79_HUMAN | Peptidyl-prolyl cis-trans isomerase... | PPIG | 487 | Annotation score: | ||
C9J679 | C9J679_HUMAN | Peptidyl-prolyl cis-trans isomerase... | PPIG | 104 | Annotation score: | ||
C9JT64 | C9JT64_HUMAN | Peptidyl-prolyl cis-trans isomerase... | PPIG | 59 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_055084 | 445 | D → E. Corresponds to variant dbSNP:rs1050354Ensembl. | 1 | |
Natural variantiVAR_055085 | 699 | N → DCombined sources2 PublicationsCorresponds to variant dbSNP:rs8207Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_009662 | 340 – 357 | RYRTP…FRRSE → VGDSFPRDLHNIAFVFLK in isoform 2. 1 PublicationAdd BLAST | 18 | |
Alternative sequenceiVSP_009663 | 358 – 754 | Missing in isoform 2. 1 PublicationAdd BLAST | 397 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U40763 mRNA Translation: AAB40347.1 X99717 mRNA Translation: CAA68053.1 AC093899 Genomic DNA Translation: AAY24119.1 AC016772 Genomic DNA Translation: AAY24233.1 CH471058 Genomic DNA Translation: EAX11267.1 CH471058 Genomic DNA Translation: EAX11268.1 CH471058 Genomic DNA Translation: EAX11269.1 CH471058 Genomic DNA Translation: EAX11270.1 BC001555 mRNA Translation: AAH01555.1 |
CCDSi | CCDS2235.1 [Q13427-1] |
PIRi | JC5314 |
RefSeqi | NP_004783.2, NM_004792.2 [Q13427-1] XP_005247023.1, XM_005246966.1 [Q13427-1] XP_005247024.1, XM_005246967.1 [Q13427-1] |
Genome annotation databases
Ensembli | ENST00000260970; ENSP00000260970; ENSG00000138398 [Q13427-1] ENST00000448752; ENSP00000407083; ENSG00000138398 [Q13427-1] ENST00000462903; ENSP00000435987; ENSG00000138398 [Q13427-2] |
GeneIDi | 9360 |
KEGGi | hsa:9360 |
UCSCi | uc002uez.4, human [Q13427-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U40763 mRNA Translation: AAB40347.1 X99717 mRNA Translation: CAA68053.1 AC093899 Genomic DNA Translation: AAY24119.1 AC016772 Genomic DNA Translation: AAY24233.1 CH471058 Genomic DNA Translation: EAX11267.1 CH471058 Genomic DNA Translation: EAX11268.1 CH471058 Genomic DNA Translation: EAX11269.1 CH471058 Genomic DNA Translation: EAX11270.1 BC001555 mRNA Translation: AAH01555.1 |
CCDSi | CCDS2235.1 [Q13427-1] |
PIRi | JC5314 |
RefSeqi | NP_004783.2, NM_004792.2 [Q13427-1] XP_005247023.1, XM_005246966.1 [Q13427-1] XP_005247024.1, XM_005246967.1 [Q13427-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2GW2 | X-ray | 1.80 | A | 1-179 | [»] | |
2WFI | X-ray | 0.75 | A | 1-177 | [»] | |
2WFJ | X-ray | 0.75 | A | 1-177 | [»] | |
5YZG | electron microscopy | 4.10 | 3 | 1-754 | [»] | |
SMRi | Q13427 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 114761, 64 interactors |
IntActi | Q13427, 50 interactors |
MINTi | Q13427 |
STRINGi | 9606.ENSP00000260970 |
Chemistry databases
BindingDBi | Q13427 |
ChEMBLi | CHEMBL3707467 |
DrugBanki | DB00172, Proline |
PTM databases
iPTMneti | Q13427 |
PhosphoSitePlusi | Q13427 |
Genetic variation databases
BioMutai | PPIG |
DMDMi | 229462749 |
Proteomic databases
EPDi | Q13427 |
jPOSTi | Q13427 |
MassIVEi | Q13427 |
MaxQBi | Q13427 |
PaxDbi | Q13427 |
PeptideAtlasi | Q13427 |
PRIDEi | Q13427 |
ProteomicsDBi | 59415 [Q13427-1] 59416 [Q13427-2] |
Protocols and materials databases
Antibodypediai | 19292, 269 antibodies |
Genome annotation databases
Ensembli | ENST00000260970; ENSP00000260970; ENSG00000138398 [Q13427-1] ENST00000448752; ENSP00000407083; ENSG00000138398 [Q13427-1] ENST00000462903; ENSP00000435987; ENSG00000138398 [Q13427-2] |
GeneIDi | 9360 |
KEGGi | hsa:9360 |
UCSCi | uc002uez.4, human [Q13427-1] |
Organism-specific databases
CTDi | 9360 |
DisGeNETi | 9360 |
GeneCardsi | PPIG |
HGNCi | HGNC:14650, PPIG |
HPAi | ENSG00000138398, Low tissue specificity |
MIMi | 606093, gene |
neXtProti | NX_Q13427 |
OpenTargetsi | ENSG00000138398 |
PharmGKBi | PA33585 |
VEuPathDBi | HostDB:ENSG00000138398.15 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0546, Eukaryota |
GeneTreei | ENSGT00940000157954 |
HOGENOMi | CLU_012062_33_6_1 |
InParanoidi | Q13427 |
OMAi | VVQTMEN |
OrthoDBi | 1403619at2759 |
PhylomeDBi | Q13427 |
TreeFami | TF318563 |
Enzyme and pathway databases
BRENDAi | 5.2.1.8, 2681 |
PathwayCommonsi | Q13427 |
Miscellaneous databases
BioGRID-ORCSi | 9360, 6 hits in 878 CRISPR screens |
ChiTaRSi | PPIG, human |
EvolutionaryTracei | Q13427 |
GeneWikii | PPIG_(gene) |
GenomeRNAii | 9360 |
Pharosi | Q13427, Tchem |
PROi | PR:Q13427 |
RNActi | Q13427, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000138398, Expressed in sural nerve and 256 other tissues |
ExpressionAtlasi | Q13427, baseline and differential |
Genevisiblei | Q13427, HS |
Family and domain databases
Gene3Di | 2.40.100.10, 1 hit |
InterProi | View protein in InterPro IPR029000, Cyclophilin-like_dom_sf IPR020892, Cyclophilin-type_PPIase_CS IPR002130, Cyclophilin-type_PPIase_dom |
Pfami | View protein in Pfam PF00160, Pro_isomerase, 1 hit |
PRINTSi | PR00153, CSAPPISMRASE |
SUPFAMi | SSF50891, SSF50891, 1 hit |
PROSITEi | View protein in PROSITE PS00170, CSA_PPIASE_1, 1 hit PS50072, CSA_PPIASE_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PPIG_HUMAN | |
Accessioni | Q13427Primary (citable) accession number: Q13427 Secondary accession number(s): D3DPC5 Q96DG9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 15, 2004 |
Last sequence update: | May 5, 2009 | |
Last modified: | February 10, 2021 | |
This is version 185 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants