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UniProtKB - Q13370 (PDE3B_HUMAN)

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Protein

cGMP-inhibited 3',5'-cyclic phosphodiesterase B

Gene

PDE3B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. May play a role in fat metabolism. Regulates cAMP binding of RAPGEF3. Through simultaneous binding to RAPGEF3 and PIK3R6 assembles a signaling complex in which the PI3K gamma complex is activated by RAPGEF3 and which is involved in angiogenesis.2 Publications

Catalytic activityi

Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate.

Cofactori

a divalent metal cation1 PublicationNote: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.1 Publication

Enzyme regulationi

Inhibited by cGMP.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei737Proton donorBy similarity1
Metal bindingi741Divalent metal cation 11
Metal bindingi821Divalent metal cation 11
Metal bindingi822Divalent metal cation 11
Metal bindingi822Divalent metal cation 21
Metal bindingi937Divalent metal cation 11

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

GO - Biological processi

  • angiogenesis Source: UniProtKB-KW
  • cellular response to insulin stimulus Source: BHF-UCL
  • G-protein coupled receptor signaling pathway Source: Reactome
  • negative regulation of angiogenesis Source: UniProtKB
  • negative regulation of cAMP-mediated signaling Source: BHF-UCL
  • negative regulation of cell adhesion Source: BHF-UCL
  • negative regulation of cell adhesion mediated by integrin Source: BHF-UCL
  • negative regulation of lipid catabolic process Source: BHF-UCL
View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionHydrolase
Biological processAngiogenesis
LigandcAMP, cGMP, Metal-binding

Enzyme and pathway databases

BRENDAi3.1.4.17 2681
ReactomeiR-HSA-165160 PDE3B signalling
R-HSA-418457 cGMP effects
R-HSA-418555 G alpha (s) signalling events
SignaLinkiQ13370
SIGNORiQ13370

Names & Taxonomyi

Protein namesi
Recommended name:
cGMP-inhibited 3',5'-cyclic phosphodiesterase B (EC:3.1.4.17)
Alternative name(s):
CGIPDE1
Short name:
CGIP1
Cyclic GMP-inhibited phosphodiesterase B
Short name:
CGI-PDE B
Gene namesi
Name:PDE3B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000152270.8
HGNCiHGNC:8779 PDE3B
MIMi602047 gene
neXtProtiNX_Q13370

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei88 – 108HelicalSequence analysisAdd BLAST21
Transmembranei117 – 137HelicalSequence analysisAdd BLAST21
Transmembranei152 – 172HelicalSequence analysisAdd BLAST21
Transmembranei192 – 212HelicalSequence analysisAdd BLAST21
Transmembranei220 – 240HelicalSequence analysisAdd BLAST21
Transmembranei247 – 267HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi2R → A: Abolishes interaction with RAPGEF3. 1 Publication1
Mutagenesisi3R → A: Abolishes interaction with RAPGEF3. 1 Publication1
Mutagenesisi6R → A: Abolishes interaction with RAPGEF3. 1 Publication1
Mutagenesisi8A → D: Impairs interaction with RAPGEF3. 1 Publication1
Mutagenesisi9K → A: Abolishes interaction with RAPGEF3. 1 Publication1
Mutagenesisi10A → D: Impairss interaction with RAPGEF3. 1 Publication1
Mutagenesisi12R → A: Abolishes interaction with RAPGEF3. 1 Publication1
Mutagenesisi439R → A: Impairss interaction with PIK3R6. 1 Publication1
Mutagenesisi440R → A: Impairss interaction with PIK3R6. 1 Publication1
Mutagenesisi445S → A: Impairss interaction with PIK3R6. 1 Publication1
Mutagenesisi449P → A: Impairss interaction with PIK3R6. 1 Publication1

Organism-specific databases

DisGeNETi5140
OpenTargetsiENSG00000152270
PharmGKBiPA33127

Chemistry databases

ChEMBLiCHEMBL290
DrugBankiDB07954 3-isobutyl-1-methyl-7H-xanthine
DB00201 Caffeine
GuidetoPHARMACOLOGYi1299

Polymorphism and mutation databases

BioMutaiPDE3B
DMDMi143811435

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001988021 – 1112cGMP-inhibited 3',5'-cyclic phosphodiesterase BAdd BLAST1112

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei13PhosphoserineBy similarity1
Modified residuei295Phosphoserine; by PKB/AKT1 or PKB/AKT2By similarity1
Modified residuei296PhosphoserineBy similarity1
Modified residuei442PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ13370
MaxQBiQ13370
PaxDbiQ13370
PeptideAtlasiQ13370
PRIDEiQ13370
ProteomicsDBi59354

PTM databases

iPTMnetiQ13370
PhosphoSitePlusiQ13370

Expressioni

Tissue specificityi

Abundant in adipose tissues.

Gene expression databases

BgeeiENSG00000152270
CleanExiHS_PDE3B
GenevisibleiQ13370 HS

Organism-specific databases

HPAiHPA056111

Interactioni

Subunit structurei

Interacts with PIK3CG (By similarity). Interacts with RAPGEF3 and PIK3R6.By similarity2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridi111166, 46 interactors
IntActiQ13370, 6 interactors
STRINGi9606.ENSP00000282096

Chemistry databases

BindingDBiQ13370

Structurei

Secondary structure

11112
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi662 – 674Combined sources13
Helixi681 – 688Combined sources8
Helixi689 – 694Combined sources6
Helixi695 – 706Combined sources12
Helixi709 – 712Combined sources4
Helixi717 – 728Combined sources12
Beta strandi735 – 738Combined sources4
Helixi739 – 752Combined sources14
Helixi802 – 804Combined sources3
Helixi808 – 820Combined sources13
Turni821 – 824Combined sources4
Helixi830 – 835Combined sources6
Helixi839 – 843Combined sources5
Turni844 – 846Combined sources3
Helixi849 – 863Combined sources15
Helixi866 – 868Combined sources3
Turni870 – 873Combined sources4
Helixi876 – 891Combined sources16
Helixi895 – 897Combined sources3
Helixi898 – 909Combined sources12
Beta strandi911 – 913Combined sources3
Helixi922 – 937Combined sources16
Helixi940 – 942Combined sources3
Helixi945 – 968Combined sources24
Helixi984 – 994Combined sources11
Helixi996 – 1005Combined sources10
Beta strandi1012 – 1015Combined sources4
Beta strandi1055 – 1058Combined sources4
Helixi1060 – 1072Combined sources13

3D structure databases

ProteinModelPortaliQ13370
SMRiQ13370
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ13370

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini651 – 1079PDEasePROSITE-ProRule annotationAdd BLAST429

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 25Interaction with RAPGEF31 PublicationAdd BLAST25
Regioni436 – 460Interaction with PIK3R61 PublicationAdd BLAST25

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1077 – 1080Poly-Glu4

Sequence similaritiesi

Belongs to the cyclic nucleotide phosphodiesterase family. PDE3 subfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IEGG Eukaryota
ENOG410XT2V LUCA
GeneTreeiENSGT00760000119066
HOGENOMiHOG000060144
HOVERGENiHBG053541
KOiK13296
OMAiCAFFFLT
OrthoDBiEOG091G0BTI
PhylomeDBiQ13370
TreeFamiTF329631

Family and domain databases

CDDicd00077 HDc, 1 hit
Gene3Di1.10.1300.10, 1 hit
InterProiView protein in InterPro
IPR003607 HD/PDEase_dom
IPR002073 PDEase_catalytic_dom
IPR036971 PDEase_catalytic_dom_sf
IPR023174 PDEase_CS
PfamiView protein in Pfam
PF00233 PDEase_I, 1 hit
SMARTiView protein in SMART
SM00471 HDc, 1 hit
PROSITEiView protein in PROSITE
PS00126 PDEASE_I_1, 1 hit
PS51845 PDEASE_I_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q13370-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRRDERDAKA MRSLQPPDGA GSPPESLRNG YVKSCVSPLR QDPPRGFFFH 
        60         70         80         90        100
LCRFCNVELR PPPASPQQPR RCSPFCRARL SLGALAAFVL ALLLGAEPES 
       110        120        130        140        150
WAAGAAWLRT LLSVCSHSLS PLFSIACAFF FLTCFLTRTK RGPGPGRSCG 
       160        170        180        190        200
SWWLLALPAC CYLGDFLVWQ WWSWPWGDGD AGSAAPHTPP EAAAGRLLLV 
       210        220        230        240        250
LSCVGLLLTL AHPLRLRHCV LVLLLASFVW WVSFTSLGSL PSALRPLLSG 
       260        270        280        290        300
LVGGAGCLLA LGLDHFFQIR EAPLHPRLSS AAEEKVPVIR PRRRSSCVSL 
       310        320        330        340        350
GETAASYYGS CKIFRRPSLP CISREQMILW DWDLKQWYKP HYQNSGGGNG 
       360        370        380        390        400
VDLSVLNEAR NMVSDLLTDP SLPPQVISSL RSISSLMGAF SGSCRPKINP 
       410        420        430        440        450
LTPFPGFYPC SEIEDPAEKG DRKLNKGLNR NSLPTPQLRR SSGTSGLLPV 
       460        470        480        490        500
EQSSRWDRNN GKRPHQEFGI SSQGCYLNGP FNSNLLTIPK QRSSSVSLTH 
       510        520        530        540        550
HVGLRRAGVL SSLSPVNSSN HGPVSTGSLT NRSPIEFPDT ADFLNKPSVI 
       560        570        580        590        600
LQRSLGNAPN TPDFYQQLRN SDSNLCNSCG HQMLKYVSTS ESDGTDCCSG 
       610        620        630        640        650
KSGEEENIFS KESFKLMETQ QEEETEKKDS RKLFQEGDKW LTEEAQSEQQ 
       660        670        680        690        700
TNIEQEVSLD LILVEEYDSL IEKMSNWNFP IFELVEKMGE KSGRILSQVM 
       710        720        730        740        750
YTLFQDTGLL EIFKIPTQQF MNYFRALENG YRDIPYHNRI HATDVLHAVW 
       760        770        780        790        800
YLTTRPVPGL QQIHNGCGTG NETDSDGRIN HGRIAYISSK SCSNPDESYG 
       810        820        830        840        850
CLSSNIPALE LMALYVAAAM HDYDHPGRTN AFLVATNAPQ AVLYNDRSVL 
       860        870        880        890        900
ENHHAASAWN LYLSRPEYNF LLHLDHVEFK RFRFLVIEAI LATDLKKHFD 
       910        920        930        940        950
FLAEFNAKAN DVNSNGIEWS NENDRLLVCQ VCIKLADING PAKVRDLHLK 
       960        970        980        990       1000
WTEGIVNEFY EQGDEEANLG LPISPFMDRS SPQLAKLQES FITHIVGPLC 
      1010       1020       1030       1040       1050
NSYDAAGLLP GQWLEAEEDN DTESGDDEDG EELDTEDEEM ENNLNPKPPR 
      1060       1070       1080       1090       1100
RKSRRRIFCQ LMHHLTENHK IWKEIVEEEE KCKADGNKLQ VENSSLPQAD 
      1110 
EIQVIEEADE EE
Length:1,112
Mass (Da):124,333
Last modified:April 3, 2007 - v2
Checksum:i55451C3DA142EF6A
GO
Isoform 2 (identifier: Q13370-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     376-426: Missing.

Note: No experimental confirmation available.
Show »
Length:1,061
Mass (Da):118,809
Checksum:i755DBD55004867DC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti84A → D in AAC50724 (PubMed:8884271).Curated1
Sequence conflicti84A → D in BAA09306 (PubMed:8706823).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03146287A → V1 PublicationCorresponds to variant dbSNP:rs1056584Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_054138376 – 426Missing in isoform 2. 1 PublicationAdd BLAST51

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38178 Genomic DNA Translation: AAC50724.1
D50640 Genomic DNA Translation: BAA09306.1
X95520 mRNA Translation: CAA64774.1
AY459346 mRNA Translation: AAR24292.1
AC018795 Genomic DNA No translation available.
AC087207 Genomic DNA No translation available.
AC090835 Genomic DNA No translation available.
CH471064 Genomic DNA Translation: EAW68474.1
BC136565 mRNA Translation: AAI36566.1
BC136566 mRNA Translation: AAI36567.1
BC144248 mRNA Translation: AAI44249.1
CCDSiCCDS7817.1 [Q13370-1]
PIRiS70522
RefSeqiNP_000913.2, NM_000922.3 [Q13370-1]
UniGeneiHs.445711

Genome annotation databases

EnsembliENST00000282096; ENSP00000282096; ENSG00000152270 [Q13370-1]
ENST00000455098; ENSP00000388644; ENSG00000152270 [Q13370-2]
GeneIDi5140
KEGGihsa:5140
UCSCiuc001mln.4 human [Q13370-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiPDE3B_HUMAN
AccessioniPrimary (citable) accession number: Q13370
Secondary accession number(s): B7ZM37
, O00639, Q14408, Q6SEI4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: April 3, 2007
Last modified: July 18, 2018
This is version 173 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

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