UniProtKB - Q13370 (PDE3B_HUMAN)
Protein
cGMP-inhibited 3',5'-cyclic phosphodiesterase B
Gene
PDE3B
Organism
Homo sapiens (Human)
Status
Functioni
Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. May play a role in fat metabolism. Regulates cAMP binding of RAPGEF3. Through simultaneous binding to RAPGEF3 and PIK3R6 assembles a signaling complex in which the PI3K gamma complex is activated by RAPGEF3 and which is involved in angiogenesis.2 Publications
Catalytic activityi
- EC:3.1.4.17
Cofactori
a divalent metal cation1 PublicationNote: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.1 Publication
Activity regulationi
Inhibited by cGMP.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 737 | Proton donorBy similarity | 1 | |
Metal bindingi | 741 | Divalent metal cation 1 | 1 | |
Metal bindingi | 821 | Divalent metal cation 1 | 1 | |
Metal bindingi | 822 | Divalent metal cation 1 | 1 | |
Metal bindingi | 822 | Divalent metal cation 2 | 1 | |
Metal bindingi | 937 | Divalent metal cation 1 | 1 |
GO - Molecular functioni
- 3',5'-cyclic-AMP phosphodiesterase activity Source: GO_Central
- 3',5'-cyclic-nucleotide phosphodiesterase activity Source: Reactome
- cGMP-inhibited cyclic-nucleotide phosphodiesterase activity Source: ProtInc
- metal ion binding Source: UniProtKB-KW
- protein kinase B binding Source: BHF-UCL
GO - Biological processi
- angiogenesis Source: UniProtKB-KW
- cellular response to insulin stimulus Source: BHF-UCL
- G protein-coupled receptor signaling pathway Source: Reactome
- negative regulation of angiogenesis Source: UniProtKB
- negative regulation of cAMP-mediated signaling Source: GO_Central
- negative regulation of cell adhesion Source: BHF-UCL
- negative regulation of cell adhesion mediated by integrin Source: BHF-UCL
- negative regulation of lipid catabolic process Source: BHF-UCL
- signal transduction Source: GO_Central
Keywordsi
Molecular function | Hydrolase |
Biological process | Angiogenesis |
Ligand | cAMP, cGMP, Metal-binding |
Enzyme and pathway databases
BRENDAi | 3.1.4.17, 2681 |
PathwayCommonsi | Q13370 |
Reactomei | R-HSA-165160, PDE3B signalling R-HSA-418555, G alpha (s) signalling events |
SignaLinki | Q13370 |
SIGNORi | Q13370 |
Names & Taxonomyi
Protein namesi | Recommended name: cGMP-inhibited 3',5'-cyclic phosphodiesterase B (EC:3.1.4.17)Alternative name(s): CGIPDE1 Short name: CGIP1 Cyclic GMP-inhibited phosphodiesterase B Short name: CGI-PDE B |
Gene namesi | Name:PDE3B |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:8779, PDE3B |
MIMi | 602047, gene |
neXtProti | NX_Q13370 |
VEuPathDBi | HostDB:ENSG00000152270.8 |
Subcellular locationi
Other locations
- Membrane Curated; Multi-pass membrane protein Curated
Cytosol
- cytosol Source: Reactome
Endoplasmic reticulum
- endoplasmic reticulum Source: BHF-UCL
Golgi apparatus
- Golgi apparatus Source: BHF-UCL
Other locations
- guanyl-nucleotide exchange factor complex Source: BHF-UCL
- integral component of membrane Source: UniProtKB-KW
- membrane Source: BHF-UCL
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 88 – 108 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 117 – 137 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 152 – 172 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 192 – 212 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 220 – 240 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 247 – 267 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 2 | R → A: Abolishes interaction with RAPGEF3. 1 Publication | 1 | |
Mutagenesisi | 3 | R → A: Abolishes interaction with RAPGEF3. 1 Publication | 1 | |
Mutagenesisi | 6 | R → A: Abolishes interaction with RAPGEF3. 1 Publication | 1 | |
Mutagenesisi | 8 | A → D: Impairs interaction with RAPGEF3. 1 Publication | 1 | |
Mutagenesisi | 9 | K → A: Abolishes interaction with RAPGEF3. 1 Publication | 1 | |
Mutagenesisi | 10 | A → D: Impairss interaction with RAPGEF3. 1 Publication | 1 | |
Mutagenesisi | 12 | R → A: Abolishes interaction with RAPGEF3. 1 Publication | 1 | |
Mutagenesisi | 439 | R → A: Impairss interaction with PIK3R6. 1 Publication | 1 | |
Mutagenesisi | 440 | R → A: Impairss interaction with PIK3R6. 1 Publication | 1 | |
Mutagenesisi | 445 | S → A: Impairss interaction with PIK3R6. 1 Publication | 1 | |
Mutagenesisi | 449 | P → A: Impairss interaction with PIK3R6. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 5140 |
OpenTargetsi | ENSG00000152270 |
PharmGKBi | PA33127 |
Miscellaneous databases
Pharosi | Q13370, Tclin |
Chemistry databases
ChEMBLi | CHEMBL290 |
DrugBanki | DB07954, 3-isobutyl-1-methyl-7H-xanthine DB01640, 6-(4-{[2-(3-iodobenzyl)-3-oxocyclohex-1-en-1-yl]amino}phenyl)-5-methyl-4,5-dihydropyridazin-3(2H)-one DB01427, Amrinone DB00201, Caffeine DB01970, Hg9a-9, Nonanoyl-N-Hydroxyethylglucamide DB09283, Trapidil |
DrugCentrali | Q13370 |
GuidetoPHARMACOLOGYi | 1299 |
Genetic variation databases
BioMutai | PDE3B |
DMDMi | 143811435 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000198802 | 1 – 1112 | cGMP-inhibited 3',5'-cyclic phosphodiesterase BAdd BLAST | 1112 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 13 | PhosphoserineBy similarity | 1 | |
Modified residuei | 295 | Phosphoserine; by PKB/AKT1 or PKB/AKT2By similarity | 1 | |
Modified residuei | 296 | PhosphoserineBy similarity | 1 | |
Modified residuei | 442 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q13370 |
jPOSTi | Q13370 |
MassIVEi | Q13370 |
MaxQBi | Q13370 |
PaxDbi | Q13370 |
PeptideAtlasi | Q13370 |
PRIDEi | Q13370 |
ProteomicsDBi | 59354 [Q13370-1] 7244 |
PTM databases
iPTMneti | Q13370 |
PhosphoSitePlusi | Q13370 |
Expressioni
Tissue specificityi
Abundant in adipose tissues.
Gene expression databases
Bgeei | ENSG00000152270, Expressed in adipose tissue and 175 other tissues |
Genevisiblei | Q13370, HS |
Organism-specific databases
HPAi | ENSG00000152270, Tissue enhanced (adipose) |
Interactioni
Subunit structurei
Interacts with PIK3CG (By similarity).
Interacts with RAPGEF3 and PIK3R6.
By similarity2 PublicationsBinary interactionsi
Hide detailsQ13370
GO - Molecular functioni
- protein kinase B binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 111166, 61 interactors |
IntActi | Q13370, 22 interactors |
STRINGi | 9606.ENSP00000282096 |
Chemistry databases
BindingDBi | Q13370 |
Miscellaneous databases
RNActi | Q13370, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q13370 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q13370 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 651 – 1079 | PDEasePROSITE-ProRule annotationAdd BLAST | 429 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 25 | Interaction with RAPGEF31 PublicationAdd BLAST | 25 | |
Regioni | 436 – 460 | Interaction with PIK3R61 PublicationAdd BLAST | 25 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1077 – 1080 | Poly-Glu | 4 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QSV8, Eukaryota |
GeneTreei | ENSGT00940000159336 |
HOGENOMi | CLU_008844_1_0_1 |
OMAi | GIEWSSE |
PhylomeDBi | Q13370 |
TreeFami | TF329631 |
Family and domain databases
CDDi | cd00077, HDc, 1 hit |
Gene3Di | 1.10.1300.10, 1 hit |
InterProi | View protein in InterPro IPR003607, HD/PDEase_dom IPR002073, PDEase_catalytic_dom IPR036971, PDEase_catalytic_dom_sf IPR023174, PDEase_CS |
Pfami | View protein in Pfam PF00233, PDEase_I, 1 hit |
SMARTi | View protein in SMART SM00471, HDc, 1 hit |
PROSITEi | View protein in PROSITE PS00126, PDEASE_I_1, 1 hit PS51845, PDEASE_I_2, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q13370-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MRRDERDAKA MRSLQPPDGA GSPPESLRNG YVKSCVSPLR QDPPRGFFFH
60 70 80 90 100
LCRFCNVELR PPPASPQQPR RCSPFCRARL SLGALAAFVL ALLLGAEPES
110 120 130 140 150
WAAGAAWLRT LLSVCSHSLS PLFSIACAFF FLTCFLTRTK RGPGPGRSCG
160 170 180 190 200
SWWLLALPAC CYLGDFLVWQ WWSWPWGDGD AGSAAPHTPP EAAAGRLLLV
210 220 230 240 250
LSCVGLLLTL AHPLRLRHCV LVLLLASFVW WVSFTSLGSL PSALRPLLSG
260 270 280 290 300
LVGGAGCLLA LGLDHFFQIR EAPLHPRLSS AAEEKVPVIR PRRRSSCVSL
310 320 330 340 350
GETAASYYGS CKIFRRPSLP CISREQMILW DWDLKQWYKP HYQNSGGGNG
360 370 380 390 400
VDLSVLNEAR NMVSDLLTDP SLPPQVISSL RSISSLMGAF SGSCRPKINP
410 420 430 440 450
LTPFPGFYPC SEIEDPAEKG DRKLNKGLNR NSLPTPQLRR SSGTSGLLPV
460 470 480 490 500
EQSSRWDRNN GKRPHQEFGI SSQGCYLNGP FNSNLLTIPK QRSSSVSLTH
510 520 530 540 550
HVGLRRAGVL SSLSPVNSSN HGPVSTGSLT NRSPIEFPDT ADFLNKPSVI
560 570 580 590 600
LQRSLGNAPN TPDFYQQLRN SDSNLCNSCG HQMLKYVSTS ESDGTDCCSG
610 620 630 640 650
KSGEEENIFS KESFKLMETQ QEEETEKKDS RKLFQEGDKW LTEEAQSEQQ
660 670 680 690 700
TNIEQEVSLD LILVEEYDSL IEKMSNWNFP IFELVEKMGE KSGRILSQVM
710 720 730 740 750
YTLFQDTGLL EIFKIPTQQF MNYFRALENG YRDIPYHNRI HATDVLHAVW
760 770 780 790 800
YLTTRPVPGL QQIHNGCGTG NETDSDGRIN HGRIAYISSK SCSNPDESYG
810 820 830 840 850
CLSSNIPALE LMALYVAAAM HDYDHPGRTN AFLVATNAPQ AVLYNDRSVL
860 870 880 890 900
ENHHAASAWN LYLSRPEYNF LLHLDHVEFK RFRFLVIEAI LATDLKKHFD
910 920 930 940 950
FLAEFNAKAN DVNSNGIEWS NENDRLLVCQ VCIKLADING PAKVRDLHLK
960 970 980 990 1000
WTEGIVNEFY EQGDEEANLG LPISPFMDRS SPQLAKLQES FITHIVGPLC
1010 1020 1030 1040 1050
NSYDAAGLLP GQWLEAEEDN DTESGDDEDG EELDTEDEEM ENNLNPKPPR
1060 1070 1080 1090 1100
RKSRRRIFCQ LMHHLTENHK IWKEIVEEEE KCKADGNKLQ VENSSLPQAD
1110
EIQVIEEADE EE
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 84 | A → D in AAC50724 (PubMed:8884271).Curated | 1 | |
Sequence conflicti | 84 | A → D in BAA09306 (PubMed:8706823).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_031462 | 87 | A → V1 PublicationCorresponds to variant dbSNP:rs1056584Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_054138 | 376 – 426 | Missing in isoform 2. 1 PublicationAdd BLAST | 51 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U38178 Genomic DNA Translation: AAC50724.1 D50640 Genomic DNA Translation: BAA09306.1 X95520 mRNA Translation: CAA64774.1 AY459346 mRNA Translation: AAR24292.1 AC018795 Genomic DNA No translation available. AC087207 Genomic DNA No translation available. AC090835 Genomic DNA No translation available. CH471064 Genomic DNA Translation: EAW68474.1 BC136565 mRNA Translation: AAI36566.1 BC136566 mRNA Translation: AAI36567.1 BC144248 mRNA Translation: AAI44249.1 |
CCDSi | CCDS7817.1 [Q13370-1] |
PIRi | S70522 |
RefSeqi | NP_000913.2, NM_000922.3 [Q13370-1] |
Genome annotation databases
Ensembli | ENST00000282096; ENSP00000282096; ENSG00000152270 [Q13370-1] ENST00000455098; ENSP00000388644; ENSG00000152270 [Q13370-2] |
GeneIDi | 5140 |
KEGGi | hsa:5140 |
UCSCi | uc001mln.4, human [Q13370-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U38178 Genomic DNA Translation: AAC50724.1 D50640 Genomic DNA Translation: BAA09306.1 X95520 mRNA Translation: CAA64774.1 AY459346 mRNA Translation: AAR24292.1 AC018795 Genomic DNA No translation available. AC087207 Genomic DNA No translation available. AC090835 Genomic DNA No translation available. CH471064 Genomic DNA Translation: EAW68474.1 BC136565 mRNA Translation: AAI36566.1 BC136566 mRNA Translation: AAI36567.1 BC144248 mRNA Translation: AAI44249.1 |
CCDSi | CCDS7817.1 [Q13370-1] |
PIRi | S70522 |
RefSeqi | NP_000913.2, NM_000922.3 [Q13370-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1SO2 | X-ray | 2.40 | A/B/C/D | 654-1073 | [»] | |
1SOJ | X-ray | 2.90 | A/B/C/D/E/F/G/H/I/J/K/L | 654-1073 | [»] | |
SMRi | Q13370 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 111166, 61 interactors |
IntActi | Q13370, 22 interactors |
STRINGi | 9606.ENSP00000282096 |
Chemistry databases
BindingDBi | Q13370 |
ChEMBLi | CHEMBL290 |
DrugBanki | DB07954, 3-isobutyl-1-methyl-7H-xanthine DB01640, 6-(4-{[2-(3-iodobenzyl)-3-oxocyclohex-1-en-1-yl]amino}phenyl)-5-methyl-4,5-dihydropyridazin-3(2H)-one DB01427, Amrinone DB00201, Caffeine DB01970, Hg9a-9, Nonanoyl-N-Hydroxyethylglucamide DB09283, Trapidil |
DrugCentrali | Q13370 |
GuidetoPHARMACOLOGYi | 1299 |
PTM databases
iPTMneti | Q13370 |
PhosphoSitePlusi | Q13370 |
Genetic variation databases
BioMutai | PDE3B |
DMDMi | 143811435 |
Proteomic databases
EPDi | Q13370 |
jPOSTi | Q13370 |
MassIVEi | Q13370 |
MaxQBi | Q13370 |
PaxDbi | Q13370 |
PeptideAtlasi | Q13370 |
PRIDEi | Q13370 |
ProteomicsDBi | 59354 [Q13370-1] 7244 |
Protocols and materials databases
Antibodypediai | 4012, 191 antibodies |
Genome annotation databases
Ensembli | ENST00000282096; ENSP00000282096; ENSG00000152270 [Q13370-1] ENST00000455098; ENSP00000388644; ENSG00000152270 [Q13370-2] |
GeneIDi | 5140 |
KEGGi | hsa:5140 |
UCSCi | uc001mln.4, human [Q13370-1] |
Organism-specific databases
CTDi | 5140 |
DisGeNETi | 5140 |
GeneCardsi | PDE3B |
HGNCi | HGNC:8779, PDE3B |
HPAi | ENSG00000152270, Tissue enhanced (adipose) |
MIMi | 602047, gene |
neXtProti | NX_Q13370 |
OpenTargetsi | ENSG00000152270 |
PharmGKBi | PA33127 |
VEuPathDBi | HostDB:ENSG00000152270.8 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QSV8, Eukaryota |
GeneTreei | ENSGT00940000159336 |
HOGENOMi | CLU_008844_1_0_1 |
OMAi | GIEWSSE |
PhylomeDBi | Q13370 |
TreeFami | TF329631 |
Enzyme and pathway databases
BRENDAi | 3.1.4.17, 2681 |
PathwayCommonsi | Q13370 |
Reactomei | R-HSA-165160, PDE3B signalling R-HSA-418555, G alpha (s) signalling events |
SignaLinki | Q13370 |
SIGNORi | Q13370 |
Miscellaneous databases
BioGRID-ORCSi | 5140, 6 hits in 874 CRISPR screens |
ChiTaRSi | PDE3B, human |
EvolutionaryTracei | Q13370 |
GenomeRNAii | 5140 |
Pharosi | Q13370, Tclin |
PROi | PR:Q13370 |
RNActi | Q13370, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000152270, Expressed in adipose tissue and 175 other tissues |
Genevisiblei | Q13370, HS |
Family and domain databases
CDDi | cd00077, HDc, 1 hit |
Gene3Di | 1.10.1300.10, 1 hit |
InterProi | View protein in InterPro IPR003607, HD/PDEase_dom IPR002073, PDEase_catalytic_dom IPR036971, PDEase_catalytic_dom_sf IPR023174, PDEase_CS |
Pfami | View protein in Pfam PF00233, PDEase_I, 1 hit |
SMARTi | View protein in SMART SM00471, HDc, 1 hit |
PROSITEi | View protein in PROSITE PS00126, PDEASE_I_1, 1 hit PS51845, PDEASE_I_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PDE3B_HUMAN | |
Accessioni | Q13370Primary (citable) accession number: Q13370 Secondary accession number(s): B7ZM37 Q6SEI4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | April 3, 2007 | |
Last modified: | February 10, 2021 | |
This is version 190 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 11
Human chromosome 11: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families