Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform

Gene

PPP2R5C

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. The PP2A-PPP2R5C holoenzyme may specifically dephosphorylate and activate TP53 and play a role in DNA damage-induced inhibition of cell proliferation. PP2A-PPP2R5C may also regulate the ERK signaling pathway through ERK dephosphorylation.2 Publications

GO - Molecular functioni

  • protein phosphatase regulator activity Source: UniProtKB

GO - Biological processi

  • DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest Source: UniProtKB
  • intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source: UniProtKB
  • negative regulation of cell proliferation Source: UniProtKB
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: MGI
  • regulation of phosphatidylinositol 3-kinase signaling Source: Reactome
  • signal transduction Source: UniProtKB

Enzyme and pathway databases

ReactomeiR-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-HSA-195253 Degradation of beta-catenin by the destruction complex
R-HSA-196299 Beta-catenin phosphorylation cascade
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2500257 Resolution of Sister Chromatid Cohesion
R-HSA-389513 CTLA4 inhibitory signaling
R-HSA-432142 Platelet sensitization by LDL
R-HSA-4641262 Disassembly of the destruction complex and recruitment of AXIN to the membrane
R-HSA-5339716 Misspliced GSK3beta mutants stabilize beta-catenin
R-HSA-5358747 S33 mutants of beta-catenin aren't phosphorylated
R-HSA-5358749 S37 mutants of beta-catenin aren't phosphorylated
R-HSA-5358751 S45 mutants of beta-catenin aren't phosphorylated
R-HSA-5358752 T41 mutants of beta-catenin aren't phosphorylated
R-HSA-5467337 APC truncation mutants have impaired AXIN binding
R-HSA-5467340 AXIN missense mutants destabilize the destruction complex
R-HSA-5467348 Truncations of AMER1 destabilize the destruction complex
R-HSA-5663220 RHO GTPases Activate Formins
R-HSA-5673000 RAF activation
R-HSA-5675221 Negative regulation of MAPK pathway
R-HSA-6804757 Regulation of TP53 Degradation
R-HSA-6811558 PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling
R-HSA-68877 Mitotic Prometaphase
SignaLinkiQ13362
SIGNORiQ13362

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform
Alternative name(s):
PP2A B subunit isoform B'-gamma
PP2A B subunit isoform B56-gamma
PP2A B subunit isoform PR61-gamma
PP2A B subunit isoform R5-gamma
Renal carcinoma antigen NY-REN-29
Gene namesi
Name:PPP2R5C
Synonyms:KIAA0044
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiHostDB:ENSG00000078304.19
HGNCiHGNC:9311 PPP2R5C
MIMi601645 gene
neXtProtiNX_Q13362

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi5527
OpenTargetsiENSG00000078304
PharmGKBiPA33674

Polymorphism and mutation databases

BioMutaiPPP2R5C
DMDMi116241235

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000714571 – 524Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoformAdd BLAST524

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Post-translational modificationi

Isoform Gamma-3 is phosphorylated on serine residues. Isoform Gamma-1 phosphorylation by ERK2 is IER3-dependent and inhibits ERK dephosphorylation by PP2A-PPP2R5C.2 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ13362
MaxQBiQ13362
PaxDbiQ13362
PeptideAtlasiQ13362
PRIDEiQ13362

PTM databases

iPTMnetiQ13362
PhosphoSitePlusiQ13362

Miscellaneous databases

PMAP-CutDBiQ13362

Expressioni

Tissue specificityi

Highest levels in heart, skeletal muscle and brain. Lower levels in pancreas, kidney, lung and placenta. Very low levels in liver.

Inductioni

Up-regulated upon DNA damage.1 Publication

Gene expression databases

BgeeiENSG00000078304
CleanExiHS_PPP2R5C
ExpressionAtlasiQ13362 baseline and differential
GenevisibleiQ13362 HS

Organism-specific databases

HPAiHPA027553

Interactioni

Subunit structurei

PP2A consists of a common heterodimeric core enzyme, composed of PPP2CA a 36 kDa catalytic subunit (subunit C) and PPP2R1A a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with PPP2CA AND PPP2R1A; the interaction is direct. Interacts with SGO1; the interaction is direct. Isoform 1 and isoform 2 interact with TP53 (phosphorylated at Ser-15 by ATM); increased upon DNA damage it drives PP2A-mediated dephosphorylation of TP53 at Thr-55. Interacts with IER3 and/or ERK kinases; regulates ERK dephosphorylation. Interacts with CIP2A; this interaction stabilizes CIP2A (PubMed:28174209).9 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi111519, 41 interactors
DIPiDIP-39401N
ELMiQ13362
IntActiQ13362, 21 interactors
MINTiQ13362
STRINGi9606.ENSP00000412324

Structurei

Secondary structure

1524
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi30 – 32Combined sources3
Turni35 – 37Combined sources3
Helixi38 – 48Combined sources11
Beta strandi55 – 57Combined sources3
Turni59 – 62Combined sources4
Helixi63 – 82Combined sources20
Beta strandi83 – 85Combined sources3
Helixi91 – 103Combined sources13
Beta strandi115 – 118Combined sources4
Helixi120 – 122Combined sources3
Helixi131 – 146Combined sources16
Helixi152 – 155Combined sources4
Turni156 – 158Combined sources3
Helixi161 – 169Combined sources9
Helixi170 – 172Combined sources3
Helixi176 – 192Combined sources17
Helixi194 – 196Combined sources3
Helixi197 – 213Combined sources17
Helixi221 – 233Combined sources13
Helixi241 – 249Combined sources9
Turni250 – 252Combined sources3
Helixi253 – 256Combined sources4
Helixi260 – 262Combined sources3
Helixi264 – 277Combined sources14
Helixi279 – 281Combined sources3
Helixi282 – 292Combined sources11
Helixi301 – 313Combined sources13
Helixi317 – 335Combined sources19
Helixi340 – 347Combined sources8
Helixi348 – 351Combined sources4
Helixi353 – 360Combined sources8
Helixi363 – 370Combined sources8
Turni372 – 374Combined sources3
Helixi384 – 398Combined sources15
Helixi409 – 430Combined sources22

3D structure databases

ProteinModelPortaliQ13362
SMRiQ13362
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ13362

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi416 – 422Nuclear localization signalSequence analysis7

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2085 Eukaryota
ENOG410XQJW LUCA
GeneTreeiENSGT00550000074525
HOGENOMiHOG000067326
HOVERGENiHBG000009
InParanoidiQ13362
KOiK11584
OMAiDIYTMKA
OrthoDBiEOG091G06HU
PhylomeDBiQ13362
TreeFamiTF105556

Family and domain databases

Gene3Di1.25.10.10, 1 hit
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR002554 PP2A_B56
PANTHERiPTHR10257 PTHR10257, 1 hit
PfamiView protein in Pfam
PF01603 B56, 1 hit
PIRSFiPIRSF028043 PP2A_B56, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Gamma-3 (identifier: Q13362-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLTCNKAGSR MVVDAANSNG PFQPVVLLHI RDVPPADQEK LFIQKLRQCC
60 70 80 90 100
VLFDFVSDPL SDLKWKEVKR AALSEMVEYI THNRNVITEP IYPEVVHMFA
110 120 130 140 150
VNMFRTLPPS SNPTGAEFDP EEDEPTLEAA WPHLQLVYEF FLRFLESPDF
160 170 180 190 200
QPNIAKKYID QKFVLQLLEL FDSEDPRERD FLKTTLHRIY GKFLGLRAYI
210 220 230 240 250
RKQINNIFYR FIYETEHHNG IAELLEILGS IINGFALPLK EEHKIFLLKV
260 270 280 290 300
LLPLHKVKSL SVYHPQLAYC VVQFLEKDST LTEPVVMALL KYWPKTHSPK
310 320 330 340 350
EVMFLNELEE ILDVIEPSEF VKIMEPLFRQ LAKCVSSPHF QVAERALYYW
360 370 380 390 400
NNEYIMSLIS DNAAKILPIM FPSLYRNSKT HWNKTIHGLI YNALKLFMEM
410 420 430 440 450
NQKLFDDCTQ QFKAEKLKEK LKMKEREEAW VKIENLAKAN PQYTVYSQAS
460 470 480 490 500
TMSIPVAMET DGPLFEDVQM LRKTVKDEAH QAQKDPKKDR PLARRKSELP
510 520
QDPHTKKALE AHCRADELAS QDGR
Length:524
Mass (Da):61,061
Last modified:October 17, 2006 - v3
Checksum:iB9CBF54550D713F8
GO
Isoform Gamma-1 (identifier: Q13362-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     443-524: YTVYSQASTM...ADELASQDGR → VLKKRIT

Show »
Length:449
Mass (Da):52,625
Checksum:i73CE22E378289FE8
GO
Isoform Gamma-2 (identifier: Q13362-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     443-481: Missing.

Show »
Length:485
Mass (Da):56,661
Checksum:i64F2D9D78F9D35B6
GO
Isoform 4 (identifier: Q13362-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-31: MLTCNKAGSRMVVDAANSNGPFQPVVLLHIR → MPNKNKKEKE...RELQKLPSLK
     443-481: Missing.

Note: No experimental confirmation available.
Show »
Length:540
Mass (Da):62,755
Checksum:i30B98C5AED1A4466
GO
Isoform 5 (identifier: Q13362-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-30: MLTCNKAGSRMVVDAANSNGPFQPVVLLHI → MPNKNKKEKE...LVLIFGGLQG

Note: No experimental confirmation available.Curated
Show »
Length:555
Mass (Da):64,292
Checksum:i1C4C9780CF86FF71
GO

Sequence cautioni

The sequence AAC50387 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAG63760 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti494R → L in AAC50387 (PubMed:8617797).Curated1
Isoform 5 (identifier: Q13362-5)
Sequence conflicti3N → K in BAG63760 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_051745515A → P. Corresponds to variant dbSNP:rs3742424Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0436451 – 31MLTCN…LLHIR → MPNKNKKEKESPKAGKSGKS SKEGQDTVESEQISVRKNSL VAVPSTVSAKIKVPVSQPIV KKDKRQNSSRFSASNNRELQ KLPSLK in isoform 4. 1 PublicationAdd BLAST31
Alternative sequenceiVSP_0467681 – 30MLTCN…VLLHI → MPNKNKKEKESPKAGKSGKS SKEGQDTVESEGTSPEEPSS PKVPPPLLPELLVLIFGGLQ G in isoform 5. CuratedAdd BLAST30
Alternative sequenceiVSP_005112443 – 524YTVYS…SQDGR → VLKKRIT in isoform Gamma-1. 2 PublicationsAdd BLAST82
Alternative sequenceiVSP_005113443 – 481Missing in isoform Gamma-2 and isoform 4. 2 PublicationsAdd BLAST39

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37352 mRNA Translation: AAC50387.1 Different initiation.
Z69030 mRNA Translation: CAA93154.1
AK131391 mRNA Translation: BAD18542.1
AK302470 mRNA Translation: BAG63760.1 Different initiation.
AB451341 mRNA Translation: BAG70155.1
AL118558 Genomic DNA No translation available.
AL137779 Genomic DNA No translation available.
CH471061 Genomic DNA Translation: EAW81756.1
D26445 mRNA Translation: BAA05465.1
L42375 mRNA Translation: AAC37603.1
CCDSiCCDS45163.1 [Q13362-2]
CCDS53911.1 [Q13362-4]
CCDS53912.1 [Q13362-5]
CCDS9964.1 [Q13362-1]
CCDS9965.1 [Q13362-3]
RefSeqiNP_001155197.1, NM_001161725.1 [Q13362-5]
NP_001155198.1, NM_001161726.1 [Q13362-4]
NP_002710.2, NM_002719.3 [Q13362-1]
NP_848701.1, NM_178586.2 [Q13362-3]
NP_848702.1, NM_178587.2 [Q13362-2]
XP_005267884.1, XM_005267827.1
XP_011535222.1, XM_011536920.1
UniGeneiHs.368264

Genome annotation databases

EnsembliENST00000328724; ENSP00000329009; ENSG00000078304 [Q13362-4]
ENST00000334743; ENSP00000333905; ENSG00000078304 [Q13362-1]
ENST00000350249; ENSP00000262239; ENSG00000078304 [Q13362-3]
ENST00000422945; ENSP00000412324; ENSG00000078304 [Q13362-5]
ENST00000445439; ENSP00000408389; ENSG00000078304 [Q13362-2]
GeneIDi5527
KEGGihsa:5527
UCSCiuc001ykk.4 human [Q13362-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry namei2A5G_HUMAN
AccessioniPrimary (citable) accession number: Q13362
Secondary accession number(s): B4DYJ8
, B5BUA5, F5GWP3, Q14391, Q15060, Q15174, Q6ZN33
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: October 17, 2006
Last modified: July 18, 2018
This is version 182 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health