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Protein

Eukaryotic translation initiation factor 3 subunit I

Gene

EIF3I

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).UniRule annotation3 Publications

GO - Molecular functioni

  • translation initiation factor activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionInitiation factor
Biological processProtein biosynthesis

Enzyme and pathway databases

ReactomeiR-HSA-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-HSA-72649 Translation initiation complex formation
R-HSA-72689 Formation of a pool of free 40S subunits
R-HSA-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-HSA-72702 Ribosomal scanning and start codon recognition
R-HSA-72706 GTP hydrolysis and joining of the 60S ribosomal subunit
SignaLinkiQ13347
SIGNORiQ13347

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 3 subunit IUniRule annotation
Short name:
eIF3iUniRule annotation
Alternative name(s):
Eukaryotic translation initiation factor 3 subunit 2UniRule annotation
TGF-beta receptor-interacting protein 1
Short name:
TRIP-1
eIF-3-betaUniRule annotation
eIF3 p36UniRule annotation
Gene namesi
Name:EIF3IUniRule annotation
Synonyms:EIF3S2UniRule annotation, TRIP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000084623.11
HGNCiHGNC:3272 EIF3I
MIMi603911 gene
neXtProtiNX_Q13347

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi8668
OpenTargetsiENSG00000084623
PharmGKBiPA162384875

Polymorphism and mutation databases

BioMutaiEIF3I
DMDMi2494895

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000510361 – 325Eukaryotic translation initiation factor 3 subunit IAdd BLAST325

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei219PhosphothreonineCombined sources1
Modified residuei264N6-acetyllysineCombined sources1
Cross-linki282Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
Modified residuei308PhosphotyrosineCombined sources1

Post-translational modificationi

Phosphorylated by TGF-beta type II receptor.UniRule annotation

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ13347
PaxDbiQ13347
PeptideAtlasiQ13347
PRIDEiQ13347
ProteomicsDBi59333

2D gel databases

DOSAC-COBS-2DPAGEiQ13347
OGPiQ13347

PTM databases

iPTMnetiQ13347
PhosphoSitePlusiQ13347
SwissPalmiQ13347

Expressioni

Gene expression databases

BgeeiENSG00000084623 Expressed in 231 organ(s), highest expression level in left adrenal gland
CleanExiHS_EIF3I
ExpressionAtlasiQ13347 baseline and differential
GenevisibleiQ13347 HS

Organism-specific databases

HPAiHPA029939
HPA029940

Interactioni

Subunit structurei

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3.UniRule annotation6 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi114217, 108 interactors
CORUMiQ13347
DIPiDIP-31116N
IntActiQ13347, 47 interactors
MINTiQ13347
STRINGi9606.ENSP00000362688

Structurei

3D structure databases

ProteinModelPortaliQ13347
SMRiQ13347
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati1 – 39WD 1Add BLAST39
Repeati43 – 81WD 2Add BLAST39
Repeati87 – 127WD 3Add BLAST41
Repeati135 – 175WD 4Add BLAST41
Repeati180 – 217WD 5Add BLAST38
Repeati221 – 267WD 6Add BLAST47
Repeati275 – 316WD 7Add BLAST42

Sequence similaritiesi

Belongs to the eIF-3 subunit I family.UniRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0643 Eukaryota
ENOG410XQ3E LUCA
GeneTreeiENSGT00630000089849
HOGENOMiHOG000231322
HOVERGENiHBG000900
InParanoidiQ13347
KOiK03246
OMAiFETRFWH
OrthoDBiEOG091G0AIZ
PhylomeDBiQ13347
TreeFamiTF101515

Family and domain databases

Gene3Di2.130.10.10, 1 hit
HAMAPiMF_03008 eIF3i, 1 hit
InterProiView protein in InterPro
IPR027525 eIF3i
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PANTHERiPTHR19877 PTHR19877, 1 hit
PfamiView protein in Pfam
PF00400 WD40, 3 hits
SMARTiView protein in SMART
SM00320 WD40, 5 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 1 hit
PS50082 WD_REPEATS_2, 4 hits
PS50294 WD_REPEATS_REGION, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q13347-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKPILLQGHE RSITQIKYNR EGDLLFTVAK DPIVNVWYSV NGERLGTYMG
60 70 80 90 100
HTGAVWCVDA DWDTKHVLTG SADNSCRLWD CETGKQLALL KTNSAVRTCG
110 120 130 140 150
FDFGGNIIMF STDKQMGYQC FVSFFDLRDP SQIDNNEPYM KIPCNDSKIT
160 170 180 190 200
SAVWGPLGEC IIAGHESGEL NQYSAKSGEV LVNVKEHSRQ INDIQLSRDM
210 220 230 240 250
TMFVTASKDN TAKLFDSTTL EHQKTFRTER PVNSAALSPN YDHVVLGGGQ
260 270 280 290 300
EAMDVTTTST RIGKFEARFF HLAFEEEFGR VKGHFGPINS VAFHPDGKSY
310 320
SSGGEDGYVR IHYFDPQYFE FEFEA
Length:325
Mass (Da):36,502
Last modified:November 1, 1996 - v1
Checksum:i02797BB72A752A96
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q5TFK1Q5TFK1_HUMAN
Eukaryotic translation initiation f...
EIF3I
125Annotation score:

Mass spectrometryi

Molecular mass is 36501.9 Da from positions 1 - 325. 1 Publication
Molecular mass is 36503.2±0.4 Da from positions 1 - 325. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39067 mRNA Translation: AAC97144.1
U36764 mRNA Translation: AAC50224.1
BC000413 mRNA Translation: AAH00413.1
BC003140 mRNA Translation: AAH03140.1
CCDSiCCDS357.1
PIRiS60335
RefSeqiNP_003748.1, NM_003757.3
UniGeneiHs.530096

Genome annotation databases

EnsembliENST00000373586; ENSP00000362688; ENSG00000084623
GeneIDi8668
KEGGihsa:8668

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39067 mRNA Translation: AAC97144.1
U36764 mRNA Translation: AAC50224.1
BC000413 mRNA Translation: AAH00413.1
BC003140 mRNA Translation: AAH03140.1
CCDSiCCDS357.1
PIRiS60335
RefSeqiNP_003748.1, NM_003757.3
UniGeneiHs.530096

3D structure databases

ProteinModelPortaliQ13347
SMRiQ13347
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114217, 108 interactors
CORUMiQ13347
DIPiDIP-31116N
IntActiQ13347, 47 interactors
MINTiQ13347
STRINGi9606.ENSP00000362688

PTM databases

iPTMnetiQ13347
PhosphoSitePlusiQ13347
SwissPalmiQ13347

Polymorphism and mutation databases

BioMutaiEIF3I
DMDMi2494895

2D gel databases

DOSAC-COBS-2DPAGEiQ13347
OGPiQ13347

Proteomic databases

EPDiQ13347
PaxDbiQ13347
PeptideAtlasiQ13347
PRIDEiQ13347
ProteomicsDBi59333

Protocols and materials databases

DNASUi8668
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000373586; ENSP00000362688; ENSG00000084623
GeneIDi8668
KEGGihsa:8668

Organism-specific databases

CTDi8668
DisGeNETi8668
EuPathDBiHostDB:ENSG00000084623.11
GeneCardsiEIF3I
HGNCiHGNC:3272 EIF3I
HPAiHPA029939
HPA029940
MIMi603911 gene
neXtProtiNX_Q13347
OpenTargetsiENSG00000084623
PharmGKBiPA162384875
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0643 Eukaryota
ENOG410XQ3E LUCA
GeneTreeiENSGT00630000089849
HOGENOMiHOG000231322
HOVERGENiHBG000900
InParanoidiQ13347
KOiK03246
OMAiFETRFWH
OrthoDBiEOG091G0AIZ
PhylomeDBiQ13347
TreeFamiTF101515

Enzyme and pathway databases

ReactomeiR-HSA-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-HSA-72649 Translation initiation complex formation
R-HSA-72689 Formation of a pool of free 40S subunits
R-HSA-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-HSA-72702 Ribosomal scanning and start codon recognition
R-HSA-72706 GTP hydrolysis and joining of the 60S ribosomal subunit
SignaLinkiQ13347
SIGNORiQ13347

Miscellaneous databases

ChiTaRSiEIF3I human
GeneWikiiEIF3I
GenomeRNAii8668
PROiPR:Q13347
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000084623 Expressed in 231 organ(s), highest expression level in left adrenal gland
CleanExiHS_EIF3I
ExpressionAtlasiQ13347 baseline and differential
GenevisibleiQ13347 HS

Family and domain databases

Gene3Di2.130.10.10, 1 hit
HAMAPiMF_03008 eIF3i, 1 hit
InterProiView protein in InterPro
IPR027525 eIF3i
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PANTHERiPTHR19877 PTHR19877, 1 hit
PfamiView protein in Pfam
PF00400 WD40, 3 hits
SMARTiView protein in SMART
SM00320 WD40, 5 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 1 hit
PS50082 WD_REPEATS_2, 4 hits
PS50294 WD_REPEATS_REGION, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiEIF3I_HUMAN
AccessioniPrimary (citable) accession number: Q13347
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: October 10, 2018
This is version 184 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  3. Translation initiation factors
    List of translation initiation factor entries
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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