UniProtKB - Q13202 (DUS8_HUMAN)
Protein
Dual specificity protein phosphatase 8
Gene
DUSP8
Organism
Homo sapiens (Human)
Status
Functioni
Has phosphatase activity with synthetic phosphatase substrates and negatively regulates mitogen-activated protein kinase activity, presumably by catalysing their dephosphorylation. Expected to display protein phosphatase activity toward phosphotyrosine, phosphoserine and phosphothreonine residues.By similarity
Catalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 246 | Phosphocysteine intermediatePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- MAP kinase tyrosine/serine/threonine phosphatase activity Source: GO_Central
- mitogen-activated protein kinase binding Source: GO_Central
- phosphatase activity Source: UniProtKB
- phosphoprotein phosphatase activity Source: GO_Central
- protein tyrosine/serine/threonine phosphatase activity Source: GO_Central
- protein tyrosine/threonine phosphatase activity Source: GO_Central
- protein tyrosine phosphatase activity Source: UniProtKB-EC
GO - Biological processi
- dephosphorylation Source: UniProtKB
- inactivation of MAPK activity Source: UniProtKB
- peptidyl-threonine dephosphorylation Source: GO_Central
- peptidyl-tyrosine dephosphorylation Source: GO_Central
- protein dephosphorylation Source: GO_Central
Keywordsi
Molecular function | Hydrolase, Protein phosphatase |
Enzyme and pathway databases
PathwayCommonsi | Q13202 |
Reactomei | R-HSA-112409, RAF-independent MAPK1/3 activation R-HSA-5675221, Negative regulation of MAPK pathway R-HSA-9652817, Signaling by MAPK mutants |
SignaLinki | Q13202 |
SIGNORi | Q13202 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:DUSP8 Synonyms:C11orf81, VH5 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000184545.10 |
HGNCi | HGNC:3074, DUSP8 |
MIMi | 602038, gene |
neXtProti | NX_Q13202 |
Pathology & Biotechi
Organism-specific databases
DisGeNETi | 1850 |
OpenTargetsi | ENSG00000184545 |
PharmGKBi | PA27531 |
Miscellaneous databases
Pharosi | Q13202, Tbio |
Polymorphism and mutation databases
BioMutai | DUSP8 |
DMDMi | 223590200 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000094810 | 1 – 625 | Dual specificity protein phosphatase 8Add BLAST | 625 |
Proteomic databases
jPOSTi | Q13202 |
MassIVEi | Q13202 |
MaxQBi | Q13202 |
PaxDbi | Q13202 |
PeptideAtlasi | Q13202 |
PRIDEi | Q13202 |
ProteomicsDBi | 59222 |
PTM databases
DEPODi | DUSP8 |
iPTMneti | Q13202 |
PhosphoSitePlusi | Q13202 |
Expressioni
Tissue specificityi
Abundant in brain, heart and skeletal muscle.1 Publication
Gene expression databases
Bgeei | ENSG00000184545, Expressed in paraflocculus and 221 other tissues |
Genevisiblei | Q13202, HS |
Organism-specific databases
HPAi | ENSG00000184545, Tissue enhanced (brain) |
Interactioni
Subunit structurei
Monomer.
1 PublicationGO - Molecular functioni
- mitogen-activated protein kinase binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 108183, 40 interactors |
IntActi | Q13202, 3 interactors |
MINTi | Q13202 |
STRINGi | 9606.ENSP00000380530 |
Miscellaneous databases
RNActi | Q13202, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q13202 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 23 – 138 | RhodanesePROSITE-ProRule annotationAdd BLAST | 116 | |
Domaini | 160 – 302 | Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd BLAST | 143 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 310 – 550 | Pro-richAdd BLAST | 241 |
Sequence similaritiesi
Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.Curated
Phylogenomic databases
eggNOGi | KOG1716, Eukaryota |
GeneTreei | ENSGT00940000160004 |
HOGENOMi | CLU_027074_16_0_1 |
InParanoidi | Q13202 |
OMAi | IQPASKM |
OrthoDBi | 1576308at2759 |
PhylomeDBi | Q13202 |
TreeFami | TF105122 |
Family and domain databases
Gene3Di | 3.40.250.10, 1 hit 3.90.190.10, 1 hit |
InterProi | View protein in InterPro IPR000340, Dual-sp_phosphatase_cat-dom IPR008343, MKP IPR029021, Prot-tyrosine_phosphatase-like IPR001763, Rhodanese-like_dom IPR036873, Rhodanese-like_dom_sf IPR016130, Tyr_Pase_AS IPR000387, TYR_PHOSPHATASE_dom IPR020422, TYR_PHOSPHATASE_DUAL_dom |
Pfami | View protein in Pfam PF00782, DSPc, 1 hit PF00581, Rhodanese, 1 hit |
PRINTSi | PR01764, MAPKPHPHTASE |
SMARTi | View protein in SMART SM00195, DSPc, 1 hit SM00450, RHOD, 1 hit |
SUPFAMi | SSF52799, SSF52799, 1 hit SSF52821, SSF52821, 1 hit |
PROSITEi | View protein in PROSITE PS50206, RHODANESE_3, 1 hit PS00383, TYR_PHOSPHATASE_1, 1 hit PS50056, TYR_PHOSPHATASE_2, 1 hit PS50054, TYR_PHOSPHATASE_DUAL, 1 hit |
i Sequence
Sequence statusi: Complete.
Q13202-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAGDRLPRKV MDAKKLASLL RGGPGGPLVI DSRSFVEYNS WHVLSSVNIC
60 70 80 90 100
CSKLVKRRLQ QGKVTIAELI QPAARSQVEA TEPQDVVVYD QSTRDASVLA
110 120 130 140 150
ADSFLSILLS KLDGCFDSVA ILTGGFATFS SCFPGLCEGK PAALLPMSLS
160 170 180 190 200
QPCLPVPSVG LTRILPHLYL GSQKDVLNKD LMTQNGISYV LNASNSCPKP
210 220 230 240 250
DFICESRFMR VPINDNYCEK LLPWLDKSIE FIDKAKLSSC QVIVHCLAGI
260 270 280 290 300
SRSATIAIAY IMKTMGMSSD DAYRFVKDRR PSISPNFNFL GQLLEYERSL
310 320 330 340 350
KLLAALQGDP GTPSGTPEPP PSPAAGAPLP RLPPPTSESA ATGNAAAREG
360 370 380 390 400
GLSAGGEPPA PPTPPATSAL QQGLRGLHLS SDRLQDTNRL KRSFSLDIKS
410 420 430 440 450
AYAPSRRPDG PGPPDPGEAP KLCKLDSPSG AALGLSSPSP DSPDAAPEAR
460 470 480 490 500
PRPRRRPRPP AGSPARSPAH SLGLNFGDAA RQTPRHGLSA LSAPGLPGPG
510 520 530 540 550
QPAGPGAWAP PLDSPGTPSP DGPWCFSPEG AQGAGGVLFA PFGRAGAPGP
560 570 580 590 600
GGGSDLRRRE AARAEPRDAR TGWPEEPAPE TQFKRRSCQM EFEEGMVEGR
610 620
ARGEELAALG KQASFSGSVE VIEVS
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 299 | S → T in AAA83151 (PubMed:7561881).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U27193 mRNA Translation: AAA83151.1 AP006285 Genomic DNA No translation available. BC038231 mRNA Translation: AAH38231.1 BC045110 mRNA Translation: AAH45110.1 |
CCDSi | CCDS7724.1 |
RefSeqi | NP_004411.2, NM_004420.2 XP_011518234.1, XM_011519932.2 XP_011518235.1, XM_011519933.2 |
Genome annotation databases
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U27193 mRNA Translation: AAA83151.1 AP006285 Genomic DNA No translation available. BC038231 mRNA Translation: AAH38231.1 BC045110 mRNA Translation: AAH45110.1 |
CCDSi | CCDS7724.1 |
RefSeqi | NP_004411.2, NM_004420.2 XP_011518234.1, XM_011519932.2 XP_011518235.1, XM_011519933.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4JMK | X-ray | 1.90 | A/B | 160-310 | [»] | |
SMRi | Q13202 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 108183, 40 interactors |
IntActi | Q13202, 3 interactors |
MINTi | Q13202 |
STRINGi | 9606.ENSP00000380530 |
PTM databases
DEPODi | DUSP8 |
iPTMneti | Q13202 |
PhosphoSitePlusi | Q13202 |
Polymorphism and mutation databases
BioMutai | DUSP8 |
DMDMi | 223590200 |
Proteomic databases
jPOSTi | Q13202 |
MassIVEi | Q13202 |
MaxQBi | Q13202 |
PaxDbi | Q13202 |
PeptideAtlasi | Q13202 |
PRIDEi | Q13202 |
ProteomicsDBi | 59222 |
Protocols and materials databases
Antibodypediai | 10181, 209 antibodies |
DNASUi | 1850 |
Genome annotation databases
Organism-specific databases
CTDi | 1850 |
DisGeNETi | 1850 |
EuPathDBi | HostDB:ENSG00000184545.10 |
GeneCardsi | DUSP8 |
HGNCi | HGNC:3074, DUSP8 |
HPAi | ENSG00000184545, Tissue enhanced (brain) |
MIMi | 602038, gene |
neXtProti | NX_Q13202 |
OpenTargetsi | ENSG00000184545 |
PharmGKBi | PA27531 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1716, Eukaryota |
GeneTreei | ENSGT00940000160004 |
HOGENOMi | CLU_027074_16_0_1 |
InParanoidi | Q13202 |
OMAi | IQPASKM |
OrthoDBi | 1576308at2759 |
PhylomeDBi | Q13202 |
TreeFami | TF105122 |
Enzyme and pathway databases
PathwayCommonsi | Q13202 |
Reactomei | R-HSA-112409, RAF-independent MAPK1/3 activation R-HSA-5675221, Negative regulation of MAPK pathway R-HSA-9652817, Signaling by MAPK mutants |
SignaLinki | Q13202 |
SIGNORi | Q13202 |
Miscellaneous databases
BioGRID-ORCSi | 1850, 6 hits in 844 CRISPR screens |
ChiTaRSi | DUSP8, human |
GenomeRNAii | 1850 |
Pharosi | Q13202, Tbio |
PROi | PR:Q13202 |
RNActi | Q13202, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000184545, Expressed in paraflocculus and 221 other tissues |
Genevisiblei | Q13202, HS |
Family and domain databases
Gene3Di | 3.40.250.10, 1 hit 3.90.190.10, 1 hit |
InterProi | View protein in InterPro IPR000340, Dual-sp_phosphatase_cat-dom IPR008343, MKP IPR029021, Prot-tyrosine_phosphatase-like IPR001763, Rhodanese-like_dom IPR036873, Rhodanese-like_dom_sf IPR016130, Tyr_Pase_AS IPR000387, TYR_PHOSPHATASE_dom IPR020422, TYR_PHOSPHATASE_DUAL_dom |
Pfami | View protein in Pfam PF00782, DSPc, 1 hit PF00581, Rhodanese, 1 hit |
PRINTSi | PR01764, MAPKPHPHTASE |
SMARTi | View protein in SMART SM00195, DSPc, 1 hit SM00450, RHOD, 1 hit |
SUPFAMi | SSF52799, SSF52799, 1 hit SSF52821, SSF52821, 1 hit |
PROSITEi | View protein in PROSITE PS50206, RHODANESE_3, 1 hit PS00383, TYR_PHOSPHATASE_1, 1 hit PS50056, TYR_PHOSPHATASE_2, 1 hit PS50054, TYR_PHOSPHATASE_DUAL, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DUS8_HUMAN | |
Accessioni | Q13202Primary (citable) accession number: Q13202 Secondary accession number(s): Q86SS8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1999 |
Last sequence update: | February 10, 2009 | |
Last modified: | December 2, 2020 | |
This is version 167 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 11
Human chromosome 11: entries, gene names and cross-references to MIM