STK3

Functionⁱ

Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1 (By similarity). Phosphorylates NEK2 and plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosome, and its ability to phosphorylate CROCC and CEP250. In conjunction with SAV1, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation. Positively regulates RAF1 activation via suppression of the inhibitory phosphorylation of RAF1 on 'Ser-259'. Phosphorylates MOBKL1A and RASSF2. Phosphorylates MOBKL1B on 'Thr-74'. Acts cooperatively with MOBKL1B to activate STK38.By similarity11 Publications

Catalytic activityⁱ

ATP + a protein = ADP + a phosphoprotein.

Cofactorⁱ

Mg²⁺

Activity regulationⁱ

Inhibited by the C-terminal non-catalytic region. Activated by caspase-cleavage. Full activation also requires homodimerization and autophosphorylation of Thr-180, which are inhibited by the proto-oncogene product RAF1. Activated by RASSF1 which acts by preventing its dephosphorylation.2 Publications

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Binding siteⁱ	56	ATP		1
Active siteⁱ	146	Proton acceptorPROSITE-ProRule annotation		1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	33 – 41	ATPPROSITE-ProRule annotation		9

GO - Molecular functionⁱ

ATP binding
Source: UniProtKB
Inferred from direct assayⁱ
- 8566796
identical protein binding
Source: IntAct
Inferred from physical interactionⁱ
- 20562859
- 23972470
magnesium ion binding
Source: UniProtKB
Inferred from direct assayⁱ
- 8566796
protein dimerization activity Source: UniProtKB
protein kinase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 28087714
protein serine/threonine kinase activator activity
Source: BHF-UCL
Traceable author statementⁱ
- 20412773
protein serine/threonine kinase activity
Source: UniProtKB
Inferred from direct assayⁱ

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

activation of protein kinase activity
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
apoptotic process
Source: UniProtKB
Traceable author statementⁱ
- 11278283
cell differentiation involved in embryonic placenta development Source: Ensembl
central nervous system development Source: Ensembl
endocardium development Source: Ensembl
hepatocyte apoptotic process Source: Ensembl
hippo signaling
Source: BHF-UCL
Inferred from direct assayⁱ
- 20412773
intracellular signal transduction
Source: UniProtKB
Inferred from direct assayⁱ
- 8566796
negative regulation of canonical Wnt signaling pathway
Source: BHF-UCL
Inferred from mutant phenotypeⁱ
- 20412773
negative regulation of cell proliferation Source: Ensembl
negative regulation of organ growth Source: Ensembl
neural tube formation Source: Ensembl
positive regulation of apoptotic process Source: UniProtKB
positive regulation of DNA-binding transcription factor activity
Source: MGI
Inferred from genetic interactionⁱ
- 22292086
positive regulation of extrinsic apoptotic signaling pathway via death domain receptors Source: Ensembl
positive regulation of fat cell differentiation Source: Ensembl
positive regulation of JNK cascade Source: Ensembl
positive regulation of protein binding
Source: MGI
Inferred from direct assayⁱ
- 22292086
positive regulation of protein kinase B signaling Source: Ensembl
primitive hemopoiesis Source: Ensembl
protein phosphorylation
Source: UniProtKB
Inferred from direct assayⁱ
protein stabilization
Source: MGI
Inferred from mutant phenotypeⁱ
- 22292086
regulation of cell differentiation involved in embryonic placenta development Source: Ensembl
signal transduction
Source: ProtInc
Traceable author statementⁱ
- 8816758
signal transduction by protein phosphorylation
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
stress-activated protein kinase signaling cascade
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Kinase, Serine/threonine-protein kinase, Transferase
Biological process	Apoptosis
Ligand	ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAⁱ	2.7.11.1 2681
Reactomeⁱ	R-HSA-2028269 Signaling by Hippo
SignaLinkⁱ	Q13188
SIGNORⁱ	Q13188

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Serine/threonine-protein kinase 3 (EC:2.7.11.1) Alternative name(s): Mammalian STE20-like protein kinase 2 Short name: MST-2 STE20-like kinase MST2 Serine/threonine-protein kinase Krs-1 Cleaved into the following 2 chains: Serine/threonine-protein kinase 3 36kDa subunit Short name: MST2/N Serine/threonine-protein kinase 3 20kDa subunit Short name: MST2/C
Gene namesⁱ	Name:STK3 Synonyms:KRS1, MST2
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 8

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000104375.15
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:11406 STK3
MIMⁱ	605030 gene
neXtProtⁱ	NX_Q13188

Keywords - Cellular componentⁱ

Cytoplasm, Nucleus

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	56	K → R: Loss of activity. 1 Publication		1
Mutagenesisⁱ	322	D → N: Resistant to proteolytic cleavage. 1 Publication		1

Organism-specific databases

DisGeNET More...DisGeNETⁱ	6788
Open Targets More...OpenTargetsⁱ	ENSG00000104375
PharmGKBⁱ	PA36213

Chemistry databases

ChEMBLⁱ	CHEMBL4708
IUPHAR/BPS Guide to PHARMACOLOGY More...GuidetoPHARMACOLOGYⁱ	2219

Polymorphism and mutation databases

BioMutaⁱ	STK3
DMDMⁱ	46577700

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Length
ChainⁱPRO_0000086689	1 – 491	Serine/threonine-protein kinase 3Add BLAST	491
ChainⁱPRO_0000413713	1 – 322	Serine/threonine-protein kinase 3 36kDa subunitAdd BLAST	322
ChainⁱPRO_0000413714	323 – 491	Serine/threonine-protein kinase 3 20kDa subunitAdd BLAST	169

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	1	N-acetylmethionineCombined sources1 Publication	1
Modified residueⁱ	15	PhosphoserineCombined sources	1
Modified residueⁱ	117	Phosphothreonine; by PKB/AKT12 Publications	1
Modified residueⁱ	180	Phosphothreonine; by autocatalysisBy similarityCurated	1
Modified residueⁱ	316	PhosphoserineCombined sources	1
Modified residueⁱ	336	PhosphothreonineCombined sources	1
Modified residueⁱ	384	Phosphothreonine; by PKB/AKT11 Publication	1
Modified residueⁱ	385	PhosphoserineCombined sources	1
Modified residueⁱ	444	PhosphoserineCombined sources	1

Post-translational modificationⁱ

Phosphorylation at Thr-117 and Thr-384 by PKB/AKT1, leads to inhibition of its: cleavage, kinase activity, autophosphorylation at Thr-180, binding to RASSF1 and nuclear translocation, and increase in its binding to RAF1.3 Publications

Proteolytically cleaved by caspase-3 during apoptosis. Proteolytic cleavage results in kinase activation and nuclear translocation of the truncated form (MST1/N).1 Publication

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Siteⁱ	322 – 323	Cleavage; by caspase-3		2

Keywords - PTMⁱ

Acetylation, Phosphoprotein

Proteomic databases

EPDⁱ	Q13188
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q13188
PaxDbⁱ	Q13188
PeptideAtlas More...PeptideAtlasⁱ	Q13188
PRIDEⁱ	Q13188
ProteomicsDBⁱ	59211

2D gel databases

USC-OGP 2-DE database More...OGPⁱ	Q13188

OGPⁱ

Q13188

PTM databases

iPTMnetⁱ	Q13188
PhosphoSitePlusⁱ	Q13188

Miscellaneous databases

PMAP-CutDBⁱ	Q13188

PMAP-CutDBⁱ

Q13188

Expressionⁱ

Tissue specificityⁱ

Expressed at high levels in adult kidney, skeletal and placenta tissues and at very low levels in adult heart, lung and brain tissues.1 Publication

Inductionⁱ

Activity increases during mitosis.1 Publication

Gene expression databases

Bgeeⁱ	ENSG00000104375 Expressed in 222 organ(s), highest expression level in chorionic villus
CleanExⁱ	HS_STK3
ExpressionAtlasⁱ	Q13188 baseline and differential
Genevisibleⁱ	Q13188 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	CAB025316 HPA007120

HPAⁱ

CAB025316
HPA007120

Interactionⁱ

Subunit structureⁱ

Homodimer; mediated via the coiled-coil region. Interacts with NORE1, which inhibits autoactivation (By similarity). Interacts with and stabilizes SAV1 (PubMed:15688006, PubMed:16930133, PubMed:28087714). Interacts with RAF1, which prevents dimerization and phosphorylation. Interacts with RASSF1. Interacts (via SARAH domain) with isoform 1 of NEK2. Interacts with ESR1 only in the presence of SAV1. Interacts with PKB/AKT1. Forms a tripartite complex with MOBKL1B and STK38. Interacts with RASSF2 (via SARAH domain). Interacts with DLG5 (via PDZ domain 3) (PubMed:28087714). Interacts with LATS1; this interaction is inhibited in the presence of DLG5 (PubMed:28087714). Interacts with MARK3 in the presence of DLG5 (PubMed:28087714).By similarity12 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
itself		13	EBI-992580,EBI-992580
FAM9B	Q8IZU0	3	EBI-992580,EBI-10175124
FLJ13057	Q53SE7	3	EBI-992580,EBI-10172181
GABARAP	O95166	2	EBI-992580,EBI-712001
GABARAPL1	Q9H0R8	2	EBI-992580,EBI-746969
GABARAPL2	P60520	2	EBI-992580,EBI-720116
GAD1	Q99259	3	EBI-992580,EBI-743184
MAP1LC3B	Q9GZQ8	5	EBI-992580,EBI-373144
MAP1LC3C	Q9BXW4	2	EBI-992580,EBI-2603996
MOB1A	Q9H8S9	7	EBI-992580,EBI-748229
MOB1B	Q7L9L4	4	EBI-992580,EBI-2558745
RAF1	P04049	6	EBI-992580,EBI-365996
RASSF1	Q9NS23-2	5	EBI-992580,EBI-438698
RASSF2	P50749	15	EBI-992580,EBI-960081
RASSF3	Q86WH2	6	EBI-992580,EBI-2845202
RASSF4	Q9H2L5	8	EBI-992580,EBI-2933362
RASSF5	Q8WWW0	7	EBI-992580,EBI-367390
RASSF5	Q8WWW0-1	6	EBI-992580,EBI-960496
SAV1	Q9H4B6	19	EBI-992580,EBI-1017775
SLMAP	Q14BN4	6	EBI-992580,EBI-1043216
STK4	Q13043	17	EBI-992580,EBI-367376
TFPT	P0C1Z6-2	3	EBI-992580,EBI-10178002
TRAF1	Q13077	5	EBI-992580,EBI-359224

GO - Molecular functionⁱ

identical protein binding
Source: IntAct
Inferred from physical interactionⁱ
- 20562859
- 23972470
protein dimerization activity Source: UniProtKB

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	112664, 95 interactors
CORUMⁱ	Q13188
Database of interacting proteins More...DIPⁱ	DIP-36128N
ELMⁱ	Q13188
IntActⁱ	Q13188, 82 interactors
Molecular INTeraction database More...MINTⁱ	Q13188
STRINGⁱ	9606.ENSP00000390500

Chemistry databases

BindingDBⁱ

Q13188

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	17 – 20	Combined sources	4
Helixⁱ	23 – 26	Combined sources	4
Beta strandⁱ	27 – 34	Combined sources	8
Beta strandⁱ	36 – 39	Combined sources	4
Beta strandⁱ	40 – 46	Combined sources	7
Turnⁱ	47 – 49	Combined sources	3
Beta strandⁱ	52 – 58	Combined sources	7
Helixⁱ	64 – 76	Combined sources	13
Beta strandⁱ	85 – 91	Combined sources	7
Beta strandⁱ	94 – 100	Combined sources	7
Beta strandⁱ	103 – 106	Combined sources	4
Helixⁱ	107 – 114	Combined sources	8
Helixⁱ	120 – 139	Combined sources	20
Helixⁱ	149 – 151	Combined sources	3
Beta strandⁱ	152 – 154	Combined sources	3
Beta strandⁱ	160 – 162	Combined sources	3
Helixⁱ	170 – 172	Combined sources	3
Helixⁱ	175 – 182	Combined sources	8
Helixⁱ	185 – 187	Combined sources	3
Helixⁱ	190 – 195	Combined sources	6
Helixⁱ	201 – 216	Combined sources	16
Turnⁱ	220 – 223	Combined sources	4
Helixⁱ	226 – 235	Combined sources	10
Helixⁱ	244 – 246	Combined sources	3
Helixⁱ	249 – 258	Combined sources	10
Helixⁱ	263 – 265	Combined sources	3
Helixⁱ	269 – 272	Combined sources	4
Helixⁱ	276 – 279	Combined sources	4
Helixⁱ	284 – 287	Combined sources	4
Helixⁱ	288 – 306	Combined sources	19
Turnⁱ	308 – 311	Combined sources	4
Beta strandⁱ	379 – 381	Combined sources	3
Helixⁱ	392 – 394	Combined sources	3
Turnⁱ	395 – 397	Combined sources	3
Helixⁱ	436 – 440	Combined sources	5
Helixⁱ	445 – 483	Combined sources	39

Show more details

3D structure databases

ProteinModelPortalⁱ	Q13188
SMRⁱ	Q13188
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	27 – 278	Protein kinasePROSITE-ProRule annotationAdd BLAST		252
Domainⁱ	437 – 484	SARAHPROSITE-ProRule annotationAdd BLAST		48

Coiled coil

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Coiled coilⁱ	287 – 328	Sequence analysisAdd BLAST		42
Coiled coilⁱ	442 – 475	Sequence analysisAdd BLAST		34

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	308 – 314	Poly-Glu		7
Compositional biasⁱ	370 – 375	Poly-Glu		6

Sequence similaritiesⁱ

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.Curated

Keywords - Domainⁱ

Coiled coil

Phylogenomic databases

eggNOGⁱ	KOG0201 Eukaryota ENOG410XP9G LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00930000150827
HOGENOMⁱ	HOG000234203
HOVERGENⁱ	HBG108518
InParanoidⁱ	Q13188
KEGG Orthology (KO) More...KOⁱ	K04412
OMAⁱ	DGAQTMI
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G065P
PhylomeDBⁱ	Q13188
TreeFamⁱ	TF354217

Family and domain databases

InterProⁱ	View protein in InterPro IPR011009 Kinase-like_dom_sf IPR024205 Mst1_SARAH_domain IPR000719 Prot_kinase_dom IPR017441 Protein_kinase_ATP_BS IPR011524 SARAH_dom
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF11629 Mst1_SARAH, 1 hit PF00069 Pkinase, 1 hit
SMARTⁱ	View protein in SMART SM00220 S_TKc, 1 hit
SUPFAMⁱ	SSF56112 SSF56112, 1 hit
PROSITEⁱ	View protein in PROSITE PS00107 PROTEIN_KINASE_ATP, 1 hit PS50011 PROTEIN_KINASE_DOM, 1 hit PS50951 SARAH, 1 hit

Sequences (2+)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show all Align All

Isoform 1 (identifier: Q13188-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEQPPAPKSK LKKLSEDSLT KQPEEVFDVL EKLGEGSYGS VFKAIHKESG 
        60         70         80         90        100
QVVAIKQVPV ESDLQEIIKE ISIMQQCDSP YVVKYYGSYF KNTDLWIVME 
       110        120        130        140        150
YCGAGSVSDI IRLRNKTLIE DEIATILKST LKGLEYLHFM RKIHRDIKAG 
       160        170        180        190        200
NILLNTEGHA KLADFGVAGQ LTDTMAKRNT VIGTPFWMAP EVIQEIGYNC 
       210        220        230        240        250
VADIWSLGIT SIEMAEGKPP YADIHPMRAI FMIPTNPPPT FRKPELWSDD 
       260        270        280        290        300
FTDFVKKCLV KNPEQRATAT QLLQHPFIKN AKPVSILRDL ITEAMEIKAK 
       310        320        330        340        350
RHEEQQRELE EEEENSDEDE LDSHTMVKTS VESVGTMRAT STMSEGAQTM 
       360        370        380        390        400
IEHNSTMLES DLGTMVINSE DEEEEDGTMK RNATSPQVQR PSFMDYFDKQ 
       410        420        430        440        450
DFKNKSHENC NQNMHEPFPM SKNVFPDNWK VPQDGDFDFL KNLSLEELQM 
       460        470        480        490 
RLKALDPMME REIEELRQRY TAKRQPILDA MDAKKRRQQN F

Length:491

Mass (Da):56,301

Last modified:April 26, 2004 - v2

Checksum:ⁱ84F598ED3617292C

GO

Isoform 2 (identifier: Q13188-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-8: MEQPPAPK → MLQLMDSGITICLRNGAASVFKKKEWSTQGEENKQD

« Hide

        10         20         30         40         50
MLQLMDSGIT ICLRNGAASV FKKKEWSTQG EENKQDSKLK KLSEDSLTKQ 
        60         70         80         90        100
PEEVFDVLEK LGEGSYGSVF KAIHKESGQV VAIKQVPVES DLQEIIKEIS 
       110        120        130        140        150
IMQQCDSPYV VKYYGSYFKN TDLWIVMEYC GAGSVSDIIR LRNKTLIEDE 
       160        170        180        190        200
IATILKSTLK GLEYLHFMRK IHRDIKAGNI LLNTEGHAKL ADFGVAGQLT 
       210        220        230        240        250
DTMAKRNTVI GTPFWMAPEV IQEIGYNCVA DIWSLGITSI EMAEGKPPYA 
       260        270        280        290        300
DIHPMRAIFM IPTNPPPTFR KPELWSDDFT DFVKKCLVKN PEQRATATQL 
       310        320        330        340        350
LQHPFIKNAK PVSILRDLIT EAMEIKAKRH EEQQRELEEE EENSDEDELD 
       360        370        380        390        400
SHTMVKTSVE SVGTMRATST MSEGAQTMIE HNSTMLESDL GTMVINSEDE 
       410        420        430        440        450
EEEDGTMKRN ATSPQVQRPS FMDYFDKQDF KNKSHENCNQ NMHEPFPMSK 
       460        470        480        490        500
NVFPDNWKVP QDGDFDFLKN LSLEELQMRL KALDPMMERE IEELRQRYTA 
       510 
KRQPILDAMD AKKRRQQNF

Note: No experimental confirmation available.

Show »

Length:519

Mass (Da):59,462

Checksum:ⁱ84CB5FEAA629AE68

GO

Computationally mapped potential isoform sequencesⁱ

There are 4 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

A0A087WZ06	A0A087WZ06_HUMAN	Serine/threonine-protein kinase 3 Serine/threonine-protein kinase 3	STK3	380	Annotation score:
E5RFQ9	E5RFQ9_HUMAN	Serine/threonine-protein kinase 3 Serine/threonine-protein kinase 3	STK3	228	Annotation score:
Q8NBU1	Q8NBU1_HUMAN	Serine/threonine-protein kinase 3 Serine/threonine-protein kinase 3 (cDNA FLJ90748 fis, clone PLACE1011824, highly similar to Human Ste20-like kinase (MST2) mRNA)	STK3	168	Annotation score:
E5RIM6	E5RIM6_HUMAN	Serine/threonine-protein kinase 3 Serine/threonine-protein kinase 3	STK3	82	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	96 – 98	WIV → YLY in CAA80909 (PubMed:8274451).Curated	3
Sequence conflictⁱ	121	D → Y in CAA80909 (PubMed:8274451).Curated	1
Sequence conflictⁱ	203	D → G in CAA80909 (PubMed:8274451).Curated	1
Sequence conflictⁱ	303	E → D in AAC50386 (PubMed:8566796).Curated	1
Sequence conflictⁱ	332 – 334	ESV → GEC in AAC50386 (PubMed:8566796).Curated	3

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱVAR_041122	60	V → L in an ovarian clear cell carcinoma sample; somatic mutation. 1 Publication		1
Natural variantⁱVAR_051670	418	F → C. Corresponds to variant dbSNP:rs36047674Ensembl.		1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_054167	1 – 8	MEQPPAPK → MLQLMDSGITICLRNGAASV FKKKEWSTQGEENKQD in isoform 2. 1 Publication		8

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U26424 mRNA Translation: AAC50386.1 U60206 mRNA Translation: AAB17261.1 AK131363 mRNA Translation: BAG54769.1 AK291837 mRNA Translation: BAF84526.1 AC016877 Genomic DNA No translation available. AP002087 Genomic DNA No translation available. AP003355 Genomic DNA No translation available. AP003467 Genomic DNA No translation available. AP003551 Genomic DNA No translation available. CH471060 Genomic DNA Translation: EAW91781.1 BC010640 mRNA Translation: AAH10640.1 Z25422 mRNA Translation: CAA80909.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS47900.1 [Q13188-1] CCDS59108.1 [Q13188-2]
PIRⁱ	I38212
NCBI Reference Sequences More...RefSeqⁱ	NP_001243241.1, NM_001256312.1 [Q13188-2] NP_006272.2, NM_006281.3 [Q13188-1]
UniGeneⁱ	Hs.492333 Hs.738869

Genome annotation databases

Ensemblⁱ	ENST00000419617; ENSP00000390500; ENSG00000104375 [Q13188-1] ENST00000523601; ENSP00000429744; ENSG00000104375 [Q13188-2]
GeneIDⁱ	6788
KEGGⁱ	hsa:6788
UCSC genome browser More...UCSCⁱ	uc003yio.5 human [Q13188-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing, Polymorphism

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q13188	Serine/threonine-protein kinase 3		RAT		491	UniRef90_Q13188
Uncharacterized protein		XENLA		612
Serine/threonine kinase 3		CASFI		491
Serine/threonine-protein kinase 3		CASCN		491
serine/threonine-protein kinase 3 isoform X1		ODORO		491
+84
Q13188-2	Uncharacterized protein		MYOLU		464	UniRef90_Q13188-2
UPI0007DC5FBD		HUMAN		576
UPI00062AAF09		NOMLE		498
UPI000D09AB54		PANTR		495
UPI000A32C450		HETGA		470
+8

Protein	Similar proteins		Species	Score	Length	Source
Q13188	Serine/threonine-protein kinase 4	)	HUMAN		487	UniRef50_Q13043
Serine/threonine-protein kinase 4		AOTNA		487
Serine/threonine-protein kinase 4		LEMCA		487
Serine/threonine-protein kinase 4		MOUSE		487
Serine/threonine-protein kinase 4		COLGU		487
+802

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U26424 mRNA Translation: AAC50386.1 U60206 mRNA Translation: AAB17261.1 AK131363 mRNA Translation: BAG54769.1 AK291837 mRNA Translation: BAF84526.1 AC016877 Genomic DNA No translation available. AP002087 Genomic DNA No translation available. AP003355 Genomic DNA No translation available. AP003467 Genomic DNA No translation available. AP003551 Genomic DNA No translation available. CH471060 Genomic DNA Translation: EAW91781.1 BC010640 mRNA Translation: AAH10640.1 Z25422 mRNA Translation: CAA80909.1
CCDSⁱ	CCDS47900.1 [Q13188-1] CCDS59108.1 [Q13188-2]
PIRⁱ	I38212
RefSeqⁱ	NP_001243241.1, NM_001256312.1 [Q13188-2] NP_006272.2, NM_006281.3 [Q13188-1]
UniGeneⁱ	Hs.492333 Hs.738869

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	3WWS	X-ray	2.01	A/B/C/D	436-484	[»]
	4HKD	X-ray	1.50	A/B/C/D	436-484	[»]
	4L0N	X-ray	1.40	A/B/C/D/E/F/G/H/I/J	436-484	[»]
	4LG4	X-ray	2.42	A/B/C/D/E/F	16-313	[»]
	4LGD	X-ray	3.05	A/B/C/D	9-491	[»]
	4OH9	X-ray	1.70	A/B	436-484	[»]
	5BRM	X-ray	2.65	G/H/I/J/K/L/M/N/O	371-401	[»]
	5DH3	X-ray	2.47	A/B	15-313	[»]
	6AO5	X-ray	2.96	A	16-491	[»]
ProteinModelPortalⁱ	Q13188
SMRⁱ	Q13188
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	112664, 95 interactors
CORUMⁱ	Q13188
DIPⁱ	DIP-36128N
ELMⁱ	Q13188
IntActⁱ	Q13188, 82 interactors
MINTⁱ	Q13188
STRINGⁱ	9606.ENSP00000390500

Chemistry databases

BindingDBⁱ	Q13188
ChEMBLⁱ	CHEMBL4708
GuidetoPHARMACOLOGYⁱ	2219

PTM databases

iPTMnetⁱ	Q13188
PhosphoSitePlusⁱ	Q13188

Polymorphism and mutation databases

BioMutaⁱ	STK3
DMDMⁱ	46577700

2D gel databases

OGPⁱ	Q13188

OGPⁱ

Q13188

Proteomic databases

EPDⁱ	Q13188
MaxQBⁱ	Q13188
PaxDbⁱ	Q13188
PeptideAtlasⁱ	Q13188
PRIDEⁱ	Q13188
ProteomicsDBⁱ	59211

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	6788
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000419617; ENSP00000390500; ENSG00000104375 [Q13188-1] ENST00000523601; ENSP00000429744; ENSG00000104375 [Q13188-2]
GeneIDⁱ	6788
KEGGⁱ	hsa:6788
UCSCⁱ	uc003yio.5 human [Q13188-1]

Organism-specific databases

CTDⁱ	6788
DisGeNETⁱ	6788
EuPathDBⁱ	HostDB:ENSG00000104375.15
GeneCardsⁱ	STK3
HGNCⁱ	HGNC:11406 STK3
HPAⁱ	CAB025316 HPA007120
MIMⁱ	605030 gene
neXtProtⁱ	NX_Q13188
OpenTargetsⁱ	ENSG00000104375
PharmGKBⁱ	PA36213
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG0201 Eukaryota ENOG410XP9G LUCA
GeneTreeⁱ	ENSGT00930000150827
HOGENOMⁱ	HOG000234203
HOVERGENⁱ	HBG108518
InParanoidⁱ	Q13188
KOⁱ	K04412
OMAⁱ	DGAQTMI
OrthoDBⁱ	EOG091G065P
PhylomeDBⁱ	Q13188
TreeFamⁱ	TF354217

Enzyme and pathway databases

BRENDAⁱ	2.7.11.1 2681
Reactomeⁱ	R-HSA-2028269 Signaling by Hippo
SignaLinkⁱ	Q13188
SIGNORⁱ	Q13188

Miscellaneous databases

ChiTaRSⁱ	STK3 human
GeneWikiⁱ	STK3
GenomeRNAiⁱ	6788
PMAP-CutDBⁱ	Q13188
Protein Ontology More...PROⁱ	PR:Q13188
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000104375 Expressed in 222 organ(s), highest expression level in chorionic villus
CleanExⁱ	HS_STK3
ExpressionAtlasⁱ	Q13188 baseline and differential
Genevisibleⁱ	Q13188 HS

Family and domain databases

InterProⁱ	View protein in InterPro IPR011009 Kinase-like_dom_sf IPR024205 Mst1_SARAH_domain IPR000719 Prot_kinase_dom IPR017441 Protein_kinase_ATP_BS IPR011524 SARAH_dom
Pfamⁱ	View protein in Pfam PF11629 Mst1_SARAH, 1 hit PF00069 Pkinase, 1 hit
SMARTⁱ	View protein in SMART SM00220 S_TKc, 1 hit
SUPFAMⁱ	SSF56112 SSF56112, 1 hit
PROSITEⁱ	View protein in PROSITE PS00107 PROTEIN_KINASE_ATP, 1 hit PS50011 PROTEIN_KINASE_DOM, 1 hit PS50951 SARAH, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	STK3_HUMAN
Accessionⁱ	Q13188Primary (citable) accession number: Q13188 Secondary accession number(s): A8K722 , B3KYA7, Q15445, Q15801, Q96FM6
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
	Last sequence update:	April 26, 2004
	Last modified:	November 7, 2018
	This is version 194 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
Human chromosome 8
Human chromosome 8: entries, gene names and cross-references to MIM
Human and mouse protein kinases
Human and mouse protein kinases: classification and index

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UniProtKB - Q13188 (STK3_HUMAN)

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Serine/threonine-protein kinase 3

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Chemistry databases

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Miscellaneous databases

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Functionⁱ

GO - Molecular functionⁱ

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Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ