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Protein

Peroxiredoxin-4

Gene

PRDX4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation.1 Publication

Miscellaneous

The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this typical 2-Cys peroxiredoxin, C(R) is provided by the other dimeric subunit to form an intersubunit disulfide. The disulfide is subsequently reduced by thioredoxin.1 Publication

Catalytic activityi

2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei124Cysteine sulfenic acid (-SOH) intermediate2 Publications1

GO - Molecular functioni

  • protein homodimerization activity Source: Ensembl
  • thioredoxin peroxidase activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionAntioxidant, Oxidoreductase, Peroxidase

Enzyme and pathway databases

BRENDAi1.11.1.15 2681
ReactomeiR-HSA-6798695 Neutrophil degranulation

Protein family/group databases

PeroxiBasei4530 Hs2CysPrx04

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxiredoxin-4 (EC:1.11.1.152 Publications)
Alternative name(s):
Antioxidant enzyme AOE372
Short name:
AOE37-2
Peroxiredoxin IV
Short name:
Prx-IV
Thioredoxin peroxidase AO372
Thioredoxin-dependent peroxide reductase A0372
Gene namesi
Name:PRDX4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

EuPathDBiHostDB:ENSG00000123131.12
HGNCiHGNC:17169 PRDX4
MIMi300927 gene
neXtProtiNX_Q13162

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum

Pathology & Biotechi

Organism-specific databases

DisGeNETi10549
OpenTargetsiENSG00000123131
PharmGKBiPA33725

Polymorphism and mutation databases

BioMutaiPRDX4
DMDMi3024727

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 37Combined sources1 PublicationAdd BLAST37
ChainiPRO_000013509838 – 271Peroxiredoxin-4Add BLAST234

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi124Interchain (with C-245); in linked formCombined sources1 Publication
Disulfide bondi245Interchain (with C-124); in linked formCombined sources1 Publication

Post-translational modificationi

The enzyme can be inactivated by further oxidation of the cysteine sulfenic acid (C(P)-SOH) to sulphinic acid (C(P)-SO2H) and sulphonic acid (C(P)-SO3H) instead of its condensation to a disulfide bond.2 Publications

Keywords - PTMi

Disulfide bond

Proteomic databases

EPDiQ13162
PaxDbiQ13162
PeptideAtlasiQ13162
PRIDEiQ13162
ProteomicsDBi59198
TopDownProteomicsiQ13162

2D gel databases

OGPiQ13162
REPRODUCTION-2DPAGEiIPI00011937
UCD-2DPAGEiQ13162

PTM databases

iPTMnetiQ13162
PhosphoSitePlusiQ13162
SwissPalmiQ13162

Expressioni

Gene expression databases

BgeeiENSG00000123131 Expressed in 232 organ(s), highest expression level in duodenum
CleanExiHS_PRDX4
ExpressionAtlasiQ13162 baseline and differential
GenevisibleiQ13162 HS

Organism-specific databases

HPAiCAB008659
CAB027389
CAB047362

Interactioni

Subunit structurei

Homodimer; disulfide-linked, upon oxidation (PubMed:9388242). 5 homodimers assemble to form a ring-like decamer (PubMed:21994946). Can form heterodimers with PRDX1 (PubMed:9388242).2 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi115800, 63 interactors
IntActiQ13162, 52 interactors
MINTiQ13162
STRINGi9606.ENSP00000368646

Structurei

Secondary structure

1271
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ13162
SMRiQ13162
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ13162

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini79 – 237ThioredoxinPROSITE-ProRule annotationAdd BLAST159

Sequence similaritiesi

Keywords - Domaini

Redox-active center, Signal

Phylogenomic databases

eggNOGiKOG0852 Eukaryota
COG0450 LUCA
GeneTreeiENSGT00390000004653
HOGENOMiHOG000022343
HOVERGENiHBG000286
InParanoidiQ13162
KOiK03386
OMAiGWKPGQD
OrthoDBiEOG091G0IE5
PhylomeDBiQ13162
TreeFamiTF105181

Family and domain databases

InterProiView protein in InterPro
IPR000866 AhpC/TSA
IPR024706 Peroxiredoxin_AhpC-typ
IPR019479 Peroxiredoxin_C
IPR036249 Thioredoxin-like_sf
IPR013766 Thioredoxin_domain
PfamiView protein in Pfam
PF10417 1-cysPrx_C, 1 hit
PF00578 AhpC-TSA, 1 hit
PIRSFiPIRSF000239 AHPC, 1 hit
SUPFAMiSSF52833 SSF52833, 1 hit
PROSITEiView protein in PROSITE
PS51352 THIOREDOXIN_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q13162-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEALPLLAAT TPDHGRHRRL LLLPLLLFLL PAGAVQGWET EERPRTREEE
60 70 80 90 100
CHFYAGGQVY PGEASRVSVA DHSLHLSKAK ISKPAPYWEG TAVIDGEFKE
110 120 130 140 150
LKLTDYRGKY LVFFFYPLDF TFVCPTEIIA FGDRLEEFRS INTEVVACSV
160 170 180 190 200
DSQFTHLAWI NTPRRQGGLG PIRIPLLSDL THQISKDYGV YLEDSGHTLR
210 220 230 240 250
GLFIIDDKGI LRQITLNDLP VGRSVDETLR LVQAFQYTDK HGEVCPAGWK
260 270
PGSETIIPDP AGKLKYFDKL N
Length:271
Mass (Da):30,540
Last modified:November 1, 1996 - v1
Checksum:i7E56B580049FC60F
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A6NG45A6NG45_HUMAN
Peroxiredoxin-4
PRDX4
186Annotation score:
A6NJJ0A6NJJ0_HUMAN
Peroxiredoxin-4
PRDX4
185Annotation score:
H7C3T4H7C3T4_HUMAN
Peroxiredoxin-4
PRDX4
161Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12P → S in CAG46469 (PubMed:15489334).Curated1
Sequence conflicti51C → Y in CAG46469 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U25182 mRNA Translation: AAB95175.1
CR541668 mRNA Translation: CAG46469.1
CR541705 mRNA Translation: CAG46506.1
BC003609 mRNA Translation: AAH03609.1
BC007107 mRNA Translation: AAH07107.1
BC016770 mRNA Translation: AAH16770.1
CCDSiCCDS14206.1
PIRiG01790
RefSeqiNP_006397.1, NM_006406.1
UniGeneiHs.83383

Genome annotation databases

EnsembliENST00000379341; ENSP00000368646; ENSG00000123131
GeneIDi10549
KEGGihsa:10549

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U25182 mRNA Translation: AAB95175.1
CR541668 mRNA Translation: CAG46469.1
CR541705 mRNA Translation: CAG46506.1
BC003609 mRNA Translation: AAH03609.1
BC007107 mRNA Translation: AAH07107.1
BC016770 mRNA Translation: AAH16770.1
CCDSiCCDS14206.1
PIRiG01790
RefSeqiNP_006397.1, NM_006406.1
UniGeneiHs.83383

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2PN8X-ray1.80A/B/C/D/E/F/G/H/I/J84-271[»]
3TJBX-ray2.38A/B/C/D/E38-271[»]
3TJFX-ray2.04A/B/C/D/E38-271[»]
3TJGX-ray2.24A/B/C/D/E38-271[»]
3TJJX-ray1.91A/B/C/D/E38-271[»]
3TJKX-ray2.09A/B/C/D/E38-271[»]
3TKPX-ray2.49A/B/C/D/E38-271[»]
3TKQX-ray2.22A/B/C/D/E38-271[»]
3TKRX-ray2.10A/B/C/D/E/F/G/H/I/J38-271[»]
3TKSX-ray2.40A/B/C/D/E38-271[»]
4RQXX-ray2.26A/B/C/D/E79-271[»]
5HQPX-ray2.60A/B38-271[»]
ProteinModelPortaliQ13162
SMRiQ13162
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115800, 63 interactors
IntActiQ13162, 52 interactors
MINTiQ13162
STRINGi9606.ENSP00000368646

Protein family/group databases

PeroxiBasei4530 Hs2CysPrx04

PTM databases

iPTMnetiQ13162
PhosphoSitePlusiQ13162
SwissPalmiQ13162

Polymorphism and mutation databases

BioMutaiPRDX4
DMDMi3024727

2D gel databases

OGPiQ13162
REPRODUCTION-2DPAGEiIPI00011937
UCD-2DPAGEiQ13162

Proteomic databases

EPDiQ13162
PaxDbiQ13162
PeptideAtlasiQ13162
PRIDEiQ13162
ProteomicsDBi59198
TopDownProteomicsiQ13162

Protocols and materials databases

DNASUi10549
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000379341; ENSP00000368646; ENSG00000123131
GeneIDi10549
KEGGihsa:10549

Organism-specific databases

CTDi10549
DisGeNETi10549
EuPathDBiHostDB:ENSG00000123131.12
GeneCardsiPRDX4
HGNCiHGNC:17169 PRDX4
HPAiCAB008659
CAB027389
CAB047362
MIMi300927 gene
neXtProtiNX_Q13162
OpenTargetsiENSG00000123131
PharmGKBiPA33725
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0852 Eukaryota
COG0450 LUCA
GeneTreeiENSGT00390000004653
HOGENOMiHOG000022343
HOVERGENiHBG000286
InParanoidiQ13162
KOiK03386
OMAiGWKPGQD
OrthoDBiEOG091G0IE5
PhylomeDBiQ13162
TreeFamiTF105181

Enzyme and pathway databases

BRENDAi1.11.1.15 2681
ReactomeiR-HSA-6798695 Neutrophil degranulation

Miscellaneous databases

ChiTaRSiPRDX4 human
EvolutionaryTraceiQ13162
GeneWikiiPRDX4
GenomeRNAii10549
PROiPR:Q13162
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000123131 Expressed in 232 organ(s), highest expression level in duodenum
CleanExiHS_PRDX4
ExpressionAtlasiQ13162 baseline and differential
GenevisibleiQ13162 HS

Family and domain databases

InterProiView protein in InterPro
IPR000866 AhpC/TSA
IPR024706 Peroxiredoxin_AhpC-typ
IPR019479 Peroxiredoxin_C
IPR036249 Thioredoxin-like_sf
IPR013766 Thioredoxin_domain
PfamiView protein in Pfam
PF10417 1-cysPrx_C, 1 hit
PF00578 AhpC-TSA, 1 hit
PIRSFiPIRSF000239 AHPC, 1 hit
SUPFAMiSSF52833 SSF52833, 1 hit
PROSITEiView protein in PROSITE
PS51352 THIOREDOXIN_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPRDX4_HUMAN
AccessioniPrimary (citable) accession number: Q13162
Secondary accession number(s): Q6FHT3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: November 7, 2018
This is version 183 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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