UniProtKB - Q13162 (PRDX4_HUMAN)
Protein
Peroxiredoxin-4
Gene
PRDX4
Organism
Homo sapiens (Human)
Status
Functioni
Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation.1 Publication
Miscellaneous
The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this typical 2-Cys peroxiredoxin, C(R) is provided by the other dimeric subunit to form an intersubunit disulfide. The disulfide is subsequently reduced by thioredoxin.1 Publication
Catalytic activityi
- EC:1.11.1.242 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 124 | Cysteine sulfenic acid (-SOH) intermediate2 Publications | 1 |
GO - Molecular functioni
- thioredoxin peroxidase activity Source: GO_Central
GO - Biological processi
- cell redox homeostasis Source: GO_Central
- extracellular matrix organization Source: Ensembl
- I-kappaB phosphorylation Source: ProtInc
- male gonad development Source: Ensembl
- negative regulation of male germ cell proliferation Source: Ensembl
- neutrophil degranulation Source: Reactome
- peptidyl-proline hydroxylation to 4-hydroxy-L-proline Source: Ensembl
- protein maturation by protein folding Source: Ensembl
- reactive oxygen species metabolic process Source: Ensembl
- response to oxidative stress Source: GO_Central
- spermatogenesis Source: Ensembl
Keywordsi
Molecular function | Antioxidant, Oxidoreductase, Peroxidase |
Enzyme and pathway databases
BRENDAi | 1.11.1.15, 2681 |
PathwayCommonsi | Q13162 |
Reactomei | R-HSA-6798695, Neutrophil degranulation |
Protein family/group databases
PeroxiBasei | 4530, Hs2CysPrx04 |
Names & Taxonomyi
Protein namesi | Recommended name: Peroxiredoxin-4 (EC:1.11.1.242 Publications)Alternative name(s): Antioxidant enzyme AOE372 Short name: AOE37-2 Peroxiredoxin IV Short name: Prx-IV Thioredoxin peroxidase AO372 Thioredoxin-dependent peroxide reductase A0372 Thioredoxin-dependent peroxiredoxin 4Curated |
Gene namesi | Name:PRDX4 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000123131.12 |
HGNCi | HGNC:17169, PRDX4 |
MIMi | 300927, gene |
neXtProti | NX_Q13162 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum 1 Publication
Other locations
- Cytoplasm 2 Publications
Note: Cotranslationally translocated to and retained within the endoplasmic reticulum. A small fraction of the protein is cytoplasmic.1 Publication
Cytosol
- cytosol Source: GO_Central
Endoplasmic reticulum
- endoplasmic reticulum Source: GO_Central
- smooth endoplasmic reticulum Source: Ensembl
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
- extracellular region Source: Reactome
Nucleus
- nucleus Source: UniProtKB
Other locations
- ficolin-1-rich granule lumen Source: Reactome
- secretory granule lumen Source: Reactome
Keywords - Cellular componenti
Cytoplasm, Endoplasmic reticulumPathology & Biotechi
Organism-specific databases
DisGeNETi | 10549 |
OpenTargetsi | ENSG00000123131 |
PharmGKBi | PA33725 |
Miscellaneous databases
Pharosi | Q13162, Tbio |
Chemistry databases
ChEMBLi | CHEMBL4295812 |
Polymorphism and mutation databases
BioMutai | PRDX4 |
DMDMi | 3024727 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 37 | Combined sources1 PublicationAdd BLAST | 37 | |
ChainiPRO_0000135098 | 38 – 271 | Peroxiredoxin-4Add BLAST | 234 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 124 | Interchain (with C-245); in linked formCombined sources1 Publication | ||
Disulfide bondi | 245 | Interchain (with C-124); in linked formCombined sources1 Publication |
Post-translational modificationi
The enzyme can be inactivated by further oxidation of the cysteine sulfenic acid (C(P)-SOH) to sulphinic acid (C(P)-SO2H) and sulphonic acid (C(P)-SO3H) instead of its condensation to a disulfide bond.2 Publications
Keywords - PTMi
Disulfide bondProteomic databases
CPTACi | CPTAC-1448 CPTAC-1449 CPTAC-1450 CPTAC-1451 CPTAC-713 CPTAC-714 |
EPDi | Q13162 |
jPOSTi | Q13162 |
MassIVEi | Q13162 |
PaxDbi | Q13162 |
PeptideAtlasi | Q13162 |
PRIDEi | Q13162 |
ProteomicsDBi | 59198 |
TopDownProteomicsi | Q13162 |
2D gel databases
OGPi | Q13162 |
REPRODUCTION-2DPAGEi | IPI00011937 |
UCD-2DPAGEi | Q13162 |
PTM databases
iPTMneti | Q13162 |
PhosphoSitePlusi | Q13162 |
SwissPalmi | Q13162 |
Expressioni
Gene expression databases
Bgeei | ENSG00000123131, Expressed in pancreas and 244 other tissues |
ExpressionAtlasi | Q13162, baseline and differential |
Genevisiblei | Q13162, HS |
Organism-specific databases
HPAi | ENSG00000123131, Group enriched (liver, pancreas) |
Interactioni
Subunit structurei
Binary interactionsi
Hide detailsQ13162
Protein-protein interaction databases
BioGRIDi | 115800, 107 interactors |
IntActi | Q13162, 63 interactors |
MINTi | Q13162 |
STRINGi | 9606.ENSP00000368646 |
Miscellaneous databases
RNActi | Q13162, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q13162 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q13162 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 79 – 237 | ThioredoxinPROSITE-ProRule annotationAdd BLAST | 159 |
Sequence similaritiesi
Keywords - Domaini
Redox-active center, SignalPhylogenomic databases
eggNOGi | KOG0852, Eukaryota |
GeneTreei | ENSGT00940000153430 |
HOGENOMi | CLU_042529_21_1_1 |
InParanoidi | Q13162 |
OMAi | EDSESCY |
OrthoDBi | 1326484at2759 |
PhylomeDBi | Q13162 |
TreeFami | TF105181 |
Family and domain databases
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR000866, AhpC/TSA IPR019479, Peroxiredoxin_C IPR036249, Thioredoxin-like_sf IPR013766, Thioredoxin_domain |
Pfami | View protein in Pfam PF10417, 1-cysPrx_C, 1 hit PF00578, AhpC-TSA, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS51352, THIOREDOXIN_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
Q13162-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEALPLLAAT TPDHGRHRRL LLLPLLLFLL PAGAVQGWET EERPRTREEE
60 70 80 90 100
CHFYAGGQVY PGEASRVSVA DHSLHLSKAK ISKPAPYWEG TAVIDGEFKE
110 120 130 140 150
LKLTDYRGKY LVFFFYPLDF TFVCPTEIIA FGDRLEEFRS INTEVVACSV
160 170 180 190 200
DSQFTHLAWI NTPRRQGGLG PIRIPLLSDL THQISKDYGV YLEDSGHTLR
210 220 230 240 250
GLFIIDDKGI LRQITLNDLP VGRSVDETLR LVQAFQYTDK HGEVCPAGWK
260 270
PGSETIIPDP AGKLKYFDKL N
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA6NJJ0 | A6NJJ0_HUMAN | Peroxiredoxin-4 | PRDX4 | 185 | Annotation score: | ||
A6NG45 | A6NG45_HUMAN | Peroxiredoxin-4 | PRDX4 | 186 | Annotation score: | ||
H7C3T4 | H7C3T4_HUMAN | Peroxiredoxin-4 | PRDX4 | 161 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 12 | P → S in CAG46469 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 51 | C → Y in CAG46469 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U25182 mRNA Translation: AAB95175.1 CR541668 mRNA Translation: CAG46469.1 CR541705 mRNA Translation: CAG46506.1 BC003609 mRNA Translation: AAH03609.1 BC007107 mRNA Translation: AAH07107.1 BC016770 mRNA Translation: AAH16770.1 |
CCDSi | CCDS14206.1 |
PIRi | G01790 |
RefSeqi | NP_006397.1, NM_006406.1 |
Genome annotation databases
Ensembli | ENST00000379341; ENSP00000368646; ENSG00000123131 |
GeneIDi | 10549 |
KEGGi | hsa:10549 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U25182 mRNA Translation: AAB95175.1 CR541668 mRNA Translation: CAG46469.1 CR541705 mRNA Translation: CAG46506.1 BC003609 mRNA Translation: AAH03609.1 BC007107 mRNA Translation: AAH07107.1 BC016770 mRNA Translation: AAH16770.1 |
CCDSi | CCDS14206.1 |
PIRi | G01790 |
RefSeqi | NP_006397.1, NM_006406.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2PN8 | X-ray | 1.80 | A/B/C/D/E/F/G/H/I/J | 84-271 | [»] | |
3TJB | X-ray | 2.38 | A/B/C/D/E | 38-271 | [»] | |
3TJF | X-ray | 2.04 | A/B/C/D/E | 38-271 | [»] | |
3TJG | X-ray | 2.24 | A/B/C/D/E | 38-271 | [»] | |
3TJJ | X-ray | 1.91 | A/B/C/D/E | 38-271 | [»] | |
3TJK | X-ray | 2.09 | A/B/C/D/E | 38-271 | [»] | |
3TKP | X-ray | 2.49 | A/B/C/D/E | 38-271 | [»] | |
3TKQ | X-ray | 2.22 | A/B/C/D/E | 38-271 | [»] | |
3TKR | X-ray | 2.10 | A/B/C/D/E/F/G/H/I/J | 38-271 | [»] | |
3TKS | X-ray | 2.40 | A/B/C/D/E | 38-271 | [»] | |
4RQX | X-ray | 2.26 | A/B/C/D/E | 79-271 | [»] | |
5HQP | X-ray | 2.60 | A/B | 38-271 | [»] | |
SMRi | Q13162 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 115800, 107 interactors |
IntActi | Q13162, 63 interactors |
MINTi | Q13162 |
STRINGi | 9606.ENSP00000368646 |
Chemistry databases
ChEMBLi | CHEMBL4295812 |
Protein family/group databases
PeroxiBasei | 4530, Hs2CysPrx04 |
PTM databases
iPTMneti | Q13162 |
PhosphoSitePlusi | Q13162 |
SwissPalmi | Q13162 |
Polymorphism and mutation databases
BioMutai | PRDX4 |
DMDMi | 3024727 |
2D gel databases
OGPi | Q13162 |
REPRODUCTION-2DPAGEi | IPI00011937 |
UCD-2DPAGEi | Q13162 |
Proteomic databases
CPTACi | CPTAC-1448 CPTAC-1449 CPTAC-1450 CPTAC-1451 CPTAC-713 CPTAC-714 |
EPDi | Q13162 |
jPOSTi | Q13162 |
MassIVEi | Q13162 |
PaxDbi | Q13162 |
PeptideAtlasi | Q13162 |
PRIDEi | Q13162 |
ProteomicsDBi | 59198 |
TopDownProteomicsi | Q13162 |
Protocols and materials databases
ABCDi | Q13162, 2 sequenced antibodies |
Antibodypediai | 3275, 429 antibodies |
CPTCi | Q13162, 5 antibodies |
DNASUi | 10549 |
Genome annotation databases
Ensembli | ENST00000379341; ENSP00000368646; ENSG00000123131 |
GeneIDi | 10549 |
KEGGi | hsa:10549 |
Organism-specific databases
CTDi | 10549 |
DisGeNETi | 10549 |
EuPathDBi | HostDB:ENSG00000123131.12 |
GeneCardsi | PRDX4 |
HGNCi | HGNC:17169, PRDX4 |
HPAi | ENSG00000123131, Group enriched (liver, pancreas) |
MIMi | 300927, gene |
neXtProti | NX_Q13162 |
OpenTargetsi | ENSG00000123131 |
PharmGKBi | PA33725 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0852, Eukaryota |
GeneTreei | ENSGT00940000153430 |
HOGENOMi | CLU_042529_21_1_1 |
InParanoidi | Q13162 |
OMAi | EDSESCY |
OrthoDBi | 1326484at2759 |
PhylomeDBi | Q13162 |
TreeFami | TF105181 |
Enzyme and pathway databases
BRENDAi | 1.11.1.15, 2681 |
PathwayCommonsi | Q13162 |
Reactomei | R-HSA-6798695, Neutrophil degranulation |
Miscellaneous databases
BioGRID-ORCSi | 10549, 10 hits in 471 CRISPR screens |
ChiTaRSi | PRDX4, human |
EvolutionaryTracei | Q13162 |
GeneWikii | PRDX4 |
GenomeRNAii | 10549 |
Pharosi | Q13162, Tbio |
PROi | PR:Q13162 |
RNActi | Q13162, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000123131, Expressed in pancreas and 244 other tissues |
ExpressionAtlasi | Q13162, baseline and differential |
Genevisiblei | Q13162, HS |
Family and domain databases
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR000866, AhpC/TSA IPR019479, Peroxiredoxin_C IPR036249, Thioredoxin-like_sf IPR013766, Thioredoxin_domain |
Pfami | View protein in Pfam PF10417, 1-cysPrx_C, 1 hit PF00578, AhpC-TSA, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS51352, THIOREDOXIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PRDX4_HUMAN | |
Accessioni | Q13162Primary (citable) accession number: Q13162 Secondary accession number(s): Q6FHT3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | November 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 199 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome X
Human chromosome X: entries, gene names and cross-references to MIM