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Protein

Replication protein A 30 kDa subunit

Gene

RPA4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi74 – 148OBAdd BLAST75

GO - Molecular functioni

  • single-stranded DNA binding Source: ProtInc

GO - Biological processi

  • DNA damage checkpoint Source: UniProtKB
  • DNA recombination Source: InterPro
  • DNA replication initiation Source: ProtInc
  • G1/S transition of mitotic cell cycle Source: Reactome
  • nucleotide-excision repair Source: UniProtKB

Keywordsi

Molecular functionDNA-binding
Biological processDNA damage, DNA repair

Enzyme and pathway databases

ReactomeiR-HSA-68962 Activation of the pre-replicative complex

Names & Taxonomyi

Protein namesi
Recommended name:
Replication protein A 30 kDa subunit
Short name:
RP-A p30
Alternative name(s):
Replication factor A protein 4
Short name:
RF-A protein 4
Gene namesi
Name:RPA4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

EuPathDBiHostDB:ENSG00000204086.4
HGNCiHGNC:30305 RPA4
MIMi300767 gene
neXtProtiNX_Q13156

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi29935
OpenTargetsiENSG00000204086
PharmGKBiPA134988548

Polymorphism and mutation databases

BioMutaiRPA4
DMDMi14917036

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000972801 – 261Replication protein A 30 kDa subunitAdd BLAST261

Proteomic databases

PaxDbiQ13156
PeptideAtlasiQ13156
PRIDEiQ13156
ProteomicsDBi59196

Expressioni

Tissue specificityi

Preferentially expressed in placental and colon mucosa. Widely expressed at intermediate or lower levels.1 Publication

Gene expression databases

BgeeiENSG00000204086
CleanExiHS_RPA4
GenevisibleiQ13156 HS

Organism-specific databases

HPAiHPA066010

Interactioni

Subunit structurei

Component of the aRPA, the alternative replication protein A complex, a trimeric complex similar to the replication protein A complex/RPA but where RPA1 and RPA3 are associated with RPA4 instead of RPA2. Interacts with RPA1 and RPA3.2 Publications

Protein-protein interaction databases

BioGridi118975, 13 interactors
DIPiDIP-24241N
IntActiQ13156, 2 interactors
STRINGi9606.ENSP00000362131

Structurei

3D structure databases

ProteinModelPortaliQ13156
SMRiQ13156
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3108 Eukaryota
COG5235 LUCA
GeneTreeiENSGT00390000010045
HOGENOMiHOG000216562
HOVERGENiHBG000086
InParanoidiQ13156
KOiK10741
OMAiTIDDFHF
OrthoDBiEOG091G0L35
PhylomeDBiQ13156
TreeFamiTF105242

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR012340 NA-bd_OB-fold
IPR014646 Rfa2/RPA32
IPR014892 RPA_C
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF08784 RPA_C, 1 hit
PIRSFiPIRSF036949 RPA32, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF50249 SSF50249, 1 hit

Sequencei

Sequence statusi: Complete.

Q13156-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKSGFGSYG SISAADGASG GSDQLCERDA TPAIKTQRPK VRIQDVVPCN
60 70 80 90 100
VNQLLSSTVF DPVFKVRGII VSQVSIVGVI RGAEKASNHI CYKIDDMTAK
110 120 130 140 150
PIEARQWFGR EKVKQVTPLS VGVYVKVFGI LKCPTGTKSL EVLKIHVLED
160 170 180 190 200
MNEFTVHILE TVNAHMMLDK ARRDTTVESV PVSPSEVNDA GDNDESHRNF
210 220 230 240 250
IQDEVLRLIH ECPHQEGKSI HELRAQLCDL SVKAIKEAID YLTVEGHIYP
260
TVDREHFKSA D
Length:261
Mass (Da):28,868
Last modified:July 11, 2001 - v2
Checksum:i6A925FAEDBE21718
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01917033A → T1 PublicationCorresponds to variant dbSNP:rs2642219Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U24186 mRNA Translation: AAB08488.2
AF494047 Genomic DNA Translation: AAM09569.1
Z86061 Genomic DNA No translation available.
BC069791 mRNA Translation: AAH69791.1
BC069808 mRNA Translation: AAH69808.1
BC069824 mRNA Translation: AAH69824.1
BC104013 mRNA Translation: AAI04014.1
BC104014 mRNA Translation: AAI04015.1
CCDSiCCDS35345.1
RefSeqiNP_037479.1, NM_013347.4
UniGeneiHs.659349

Genome annotation databases

EnsembliENST00000373040; ENSP00000362131; ENSG00000204086
GeneIDi29935
KEGGihsa:29935
UCSCiuc004efv.5 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiRFA4_HUMAN
AccessioniPrimary (citable) accession number: Q13156
Secondary accession number(s): Q3SY03
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 11, 2001
Last modified: June 20, 2018
This is version 132 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

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