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UniProtKB - Q13131 (AAPK1_HUMAN)

Entry version 227 (23 Feb 2022)
Sequence version 4 (28 Jul 2009)
Previous versions | rss
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Protein

5'-AMP-activated protein kinase catalytic subunit alpha-1

Gene

PRKAA1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism (PubMed:17307971, PubMed:17712357).

In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation (PubMed:17307971, PubMed:17712357).

AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators (PubMed:17307971, PubMed:17712357).

Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively (By similarity).

Promotes lipolysis of lipid droplets by mediating phosphorylation of isoform 1 of CHKA (CHKalpha2) (PubMed:34077757).

Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3 (By similarity).

AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160 (By similarity).

Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A (PubMed:11554766, PubMed:11518699, PubMed:15866171, PubMed:17711846, PubMed:18184930).

Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm (By similarity).

In response to stress, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), leading to promote transcription (By similarity).

Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1/ULK1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2 (PubMed:14651849, PubMed:18439900, PubMed:20160076, PubMed:21205641).

In response to nutrient limitation, promotes autophagy by phosphorylating and activating ATG1/ULK1 (PubMed:21205641).

In that process also activates WDR45/WIPI4 (PubMed:28561066).

Phosphorylates CASP6, thereby preventing its autoprocessing and subsequent activation (PubMed:32029622).

In response to nutrient limitation, phosphorylates transcription factor FOXO3 promoting FOXO3 mitochondrial import (By similarity).

Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin (PubMed:17486097).

AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it (By similarity).

May regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it (By similarity).

Also has tau-protein kinase activity: in response to amyloid beta A4 protein (APP) exposure, activated by CAMKK2, leading to phosphorylation of MAPT/TAU; however the relevance of such data remains unclear in vivo (By similarity).

Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1 (PubMed:20074060, PubMed:12519745).

2 PublicationsBy similarity15 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Mg2+

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Activated by phosphorylation on Thr-183. Binding of AMP to non-catalytic gamma subunit (PRKAG1, PRKAG2 or PRKAG3) results in allosteric activation, inducing phosphorylation on Thr-183. AMP-binding to gamma subunit also sustains activity by preventing dephosphorylation of Thr-183. ADP also stimulates Thr-183 phosphorylation, without stimulating already phosphorylated AMPK. ATP promotes dephosphorylation of Thr-183, rendering the enzyme inactive. Under physiological conditions AMPK mainly exists in its inactive form in complex with ATP, which is much more abundant than AMP. AMPK is activated by antihyperglycemic drug metformin, a drug prescribed to patients with type 2 diabetes: in vivo, metformin seems to mainly inhibit liver gluconeogenesis. However, metformin can be used to activate AMPK in muscle and other cells in culture or ex vivo (PubMed:11602624). Selectively inhibited by compound C (6-[4-(2-Piperidin-1-yl-ethoxy)-phenyl)]-3-pyridin-4-yl-pyyrazolo[1,5-a] pyrimidine. Activated by resveratrol, a natural polyphenol present in red wine, and S17834, a synthetic polyphenol.5 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei56ATPPROSITE-ProRule annotation1
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei150Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi33 – 41ATPPROSITE-ProRule annotation9

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionChromatin regulator, Kinase, Serine/threonine-protein kinase, Transferase
Biological processAutophagy, Biological rhythms, Cholesterol biosynthesis, Cholesterol metabolism, Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism, Transcription, Transcription regulation, Wnt signaling pathway
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDA Comprehensive Enzyme Information System

More...BRENDAi		2.7.11.1, 2681
2.7.11.31, 2681

Pathway Commons web resource for biological pathway data

More...PathwayCommonsi		Q13131

Reactome - a knowledgebase of biological pathways and processes

More...Reactomei		R-HSA-1632852, Macroautophagy
R-HSA-380972, Energy dependent regulation of mTOR by LKB1-AMPK
R-HSA-5628897, TP53 Regulates Metabolic Genes
R-HSA-6804756, Regulation of TP53 Activity through Phosphorylation
R-HSA-9619483, Activation of AMPK downstream of NMDARs

SignaLink: a signaling pathway resource with multi-layered regulatory networks

More...SignaLinki		Q13131

SIGNOR Signaling Network Open Resource

More...SIGNORi		Q13131

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
5'-AMP-activated protein kinase catalytic subunit alpha-1 (EC:2.7.11.11 Publication)
Short name:
AMPK subunit alpha-1
Alternative name(s):
Acetyl-CoA carboxylase kinase (EC:2.7.11.27By similarity)
Short name:
ACACA kinase
Hydroxymethylglutaryl-CoA reductase kinase (EC:2.7.11.31By similarity)
Short name:
HMGCR kinase
Tau-protein kinase PRKAA1 (EC:2.7.11.26By similarity)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:PRKAA1
Synonyms:AMPK1
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 5

Organism-specific databases

Human Gene Nomenclature Database

More...HGNCi		HGNC:9376, PRKAA1

Online Mendelian Inheritance in Man (OMIM)

More...MIMi		602739, gene

neXtProt; the human protein knowledge platform

More...neXtProti		NX_Q13131

Eukaryotic Pathogen, Vector and Host Database Resources

More...VEuPathDBi		HostDB:ENSG00000132356

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cytoplasm, Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi307V → G or Q: Activates the kinase activity. 1 Publication1

Organism-specific databases

DisGeNET

More...DisGeNETi		5562

Open Targets

More...OpenTargetsi		ENSG00000132356

The Pharmacogenetics and Pharmacogenomics Knowledge Base

More...PharmGKBi		PA33744

Miscellaneous databases

Pharos NIH Druggable Genome Knowledgebase

More...Pharosi		Q13131, Tclin

Chemistry databases

ChEMBL database of bioactive drug-like small molecules

More...ChEMBLi		CHEMBL4045

Drug and drug target database

More...DrugBanki		DB00945, Acetylsalicylic acid
DB00131, Adenosine phosphate
DB00171, ATP
DB12010, Fostamatinib
DB00914, Phenformin
DB00273, Topiramate

DrugCentral

More...DrugCentrali		Q13131

Genetic variation databases

BioMuta curated single-nucleotide variation and disease association database

More...BioMutai		PRKAA1

Domain mapping of disease mutations (DMDM)

More...DMDMi		254763436

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000855891 – 5595'-AMP-activated protein kinase catalytic subunit alpha-1Add BLAST559

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei32PhosphothreonineCombined sources1
Modified residuei183Phosphothreonine; by LKB1 and CaMKK2By similarity1
Modified residuei269PhosphothreonineBy similarity1
Modified residuei355PhosphothreonineCombined sources1
Modified residuei356PhosphoserineCombined sources1
Modified residuei360Phosphoserine; by ULK1By similarity1
Modified residuei368Phosphothreonine; by ULK1By similarity1
Modified residuei382PhosphothreonineCombined sources1
Modified residuei397PhosphoserineBy similarity1
Modified residuei467PhosphoserineCombined sources1
Modified residuei486PhosphoserineCombined sources1
Modified residuei488PhosphothreonineBy similarity1
Modified residuei490PhosphothreonineCombined sources1
Modified residuei496PhosphoserineCombined sources1
Modified residuei508PhosphoserineCombined sources1
Modified residuei524PhosphoserineCombined sources1
Modified residuei527PhosphoserineCombined sources1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Ubiquitinated.By similarity
Phosphorylated at Thr-183 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Also phosphorylated at Thr-183 by CAMKK2; triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio. CAMKK1 can also phosphorylate Thr-183, but at a much lower level. Dephosphorylated by protein phosphatase 2A and 2C (PP2A and PP2C). Phosphorylated by ULK1 and ULK2; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1, ULK2 and AMPK. Dephosphorylated by PPM1A and PPM1B.4 Publications

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

Encyclopedia of Proteome Dynamics

More...EPDi		Q13131

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		Q13131

MassIVE - Mass Spectrometry Interactive Virtual Environment

More...MassIVEi		Q13131

MaxQB - The MaxQuant DataBase

More...MaxQBi		Q13131

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		Q13131

PeptideAtlas

More...PeptideAtlasi		Q13131

PRoteomics IDEntifications database

More...PRIDEi		Q13131

ProteomicsDB: a multi-organism proteome resource

More...ProteomicsDBi		59180 [Q13131-1]
59181 [Q13131-2]

PTM databases

GlyGen: Computational and Informatics Resources for Glycoscience

More...GlyGeni		Q13131, 1 site, 1 O-linked glycan (1 site)

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		Q13131

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...PhosphoSitePlusi		Q13131

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...Bgeei		ENSG00000132356, Expressed in testis and 229 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...ExpressionAtlasi		Q13131, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...Genevisiblei		Q13131, HS

Organism-specific databases

Human Protein Atlas

More...HPAi		ENSG00000132356, Low tissue specificity

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3) (PubMed:21680840).

Interacts with FNIP1 and FNIP2 (PubMed:17028174, PubMed:18403135, PubMed:18663353).

4 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Hide details

GO - Molecular functioni

Complete GO annotation on QuickGO ...

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		111549, 234 interactors

ComplexPortal: manually curated resource of macromolecular complexes

More...ComplexPortali		CPX-5633, AMPK complex, alpha1-beta1-gamma1 variant
CPX-5786, AMPK complex, alpha1-beta1-gamma2 variant
CPX-5791, AMPK complex, alpha1-beta2-gamma1 variant
CPX-5841, AMPK complex, alpha1-beta2-gamma3 variant
CPX-5842, AMPK complex, alpha1-beta1-gamma3 variant
CPX-5846, AMPK complex, alpha1-beta2-gamma2 variant

CORUM comprehensive resource of mammalian protein complexes

More...CORUMi		Q13131

Database of interacting proteins

More...DIPi		DIP-39973N

Protein interaction database and analysis system

More...IntActi		Q13131, 118 interactors

Molecular INTeraction database

More...MINTi		Q13131

STRING: functional protein association networks

More...STRINGi		9606.ENSP00000346148

Chemistry databases

BindingDB database of measured binding affinities

More...BindingDBi		Q13131

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...RNActi		Q13131, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1559
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		Q13131

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini27 – 279Protein kinasePROSITE-ProRule annotationAdd BLAST253

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni302 – 381AISAdd BLAST80
Regioni485 – 536DisorderedSequence analysisAdd BLAST52

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The AIS (autoinhibitory sequence) region shows some sequence similarity with the ubiquitin-associated domains and represses kinase activity.2 Publications

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		KOG0583, Eukaryota

Ensembl GeneTree

More...GeneTreei		ENSGT00940000158865

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_000288_59_3_1

Identification of Orthologs from Complete Genome Data

More...OMAi		YRSCQKD

Database of Orthologous Groups

More...OrthoDBi		1127668at2759

Database for complete collections of gene phylogenies

More...PhylomeDBi		Q13131

TreeFam database of animal gene trees

More...TreeFami		TF314032

Family and domain databases

Conserved Domains Database

More...CDDi		cd12199, AMPKA1_C, 1 hit
cd14403, UBA_AID_AAPK1, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR032270, AMPK_C
IPR039137, AMPKA1_C
IPR028375, KA1/Ssp2_C
IPR011009, Kinase-like_dom_sf
IPR028797, PRKAA1_UBA
IPR000719, Prot_kinase_dom
IPR017441, Protein_kinase_ATP_BS
IPR008271, Ser/Thr_kinase_AS

Pfam protein domain database

More...Pfami		View protein in Pfam
PF16579, AdenylateSensor, 1 hit
PF00069, Pkinase, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...SMARTi		View protein in SMART
SM00220, S_TKc, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF103243, SSF103243, 1 hit
SSF56112, SSF56112, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS00107, PROTEIN_KINASE_ATP, 1 hit
PS50011, PROTEIN_KINASE_DOM, 1 hit
PS00108, PROTEIN_KINASE_ST, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 2 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q13131-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <p><strong>What is the canonical sequence?</strong><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MRRLSSWRKM ATAEKQKHDG RVKIGHYILG DTLGVGTFGK VKVGKHELTG 
        60         70         80         90        100
HKVAVKILNR QKIRSLDVVG KIRREIQNLK LFRHPHIIKL YQVISTPSDI 
       110        120        130        140        150
FMVMEYVSGG ELFDYICKNG RLDEKESRRL FQQILSGVDY CHRHMVVHRD 
       160        170        180        190        200
LKPENVLLDA HMNAKIADFG LSNMMSDGEF LRTSCGSPNY AAPEVISGRL 
       210        220        230        240        250
YAGPEVDIWS SGVILYALLC GTLPFDDDHV PTLFKKICDG IFYTPQYLNP 
       260        270        280        290        300
SVISLLKHML QVDPMKRATI KDIREHEWFK QDLPKYLFPE DPSYSSTMID 
       310        320        330        340        350
DEALKEVCEK FECSEEEVLS CLYNRNHQDP LAVAYHLIID NRRIMNEAKD 
       360        370        380        390        400
FYLATSPPDS FLDDHHLTRP HPERVPFLVA ETPRARHTLD ELNPQKSKHQ 
       410        420        430        440        450
GVRKAKWHLG IRSQSRPNDI MAEVCRAIKQ LDYEWKVVNP YYLRVRRKNP 
       460        470        480        490        500
VTSTYSKMSL QLYQVDSRTY LLDFRSIDDE ITEAKSGTAT PQRSGSVSNY 
       510        520        530        540        550
RSCQRSDSDA EAQGKSSEVS LTSSVTSLDS SPVDLTPRPG SHTIEFFEMC 

ANLIKILAQ
Length:559
Mass (Da):64,009
Last modified:July 28, 2009 - v4
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iABAE71FBF912947A
GO
Isoform 2 (identifier: Q13131-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     121-121: R → RKSDVPGVVKTGSTKE

Show »
Length:574
Mass (Da):65,523
Checksum:i8EA8B85393F48DAA
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q96E92Q96E92_HUMAN
5'-AMP-activated protein kinase cat...
PRKAA1 hCG_37338
207Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAA64850 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence AAD43027 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence AAH37303 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence BAA36547 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence BAG35788 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti5S → C in BAG35788 (PubMed:14702039).Curated1
Sequence conflicti9K → S in AAD43027 (PubMed:11042152).Curated1
Sequence conflicti37T → A in AAA64850 (Ref. 6) Curated1
Sequence conflicti202A → V in AAA64850 (Ref. 6) Curated1
Sequence conflicti208I → L in AAA64850 (Ref. 6) Curated1
Sequence conflicti269T → S in BAA36547 (Ref. 3) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_05840110M → L1 PublicationCorresponds to variant dbSNP:rs17855679Ensembl.1
Natural variantiVAR_03562216Q → R in a breast cancer sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs928784854Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_035431121R → RKSDVPGVVKTGSTKE in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
AC008810 Genomic DNA No translation available.
BC048980 mRNA Translation: AAH48980.1
AB022017 mRNA Translation: BAA36547.1 Different initiation.
AK312947 mRNA Translation: BAG35788.1 Different initiation.
BC037303 mRNA Translation: AAH37303.1 Different initiation.
AF100763 mRNA Translation: AAD43027.1 Different initiation.
U22456 mRNA Translation: AAA64850.1 Different initiation.
Y12856 mRNA Translation: CAA73361.1

The Consensus CDS (CCDS) project

More...CCDSi		CCDS3932.2 [Q13131-1]
CCDS3933.2 [Q13131-2]

Protein sequence database of the Protein Information Resource

More...PIRi		G01743

NCBI Reference Sequences

More...RefSeqi		NP_006242.5, NM_006251.5 [Q13131-1]
NP_996790.3, NM_206907.3 [Q13131-2]

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...Ensembli		ENST00000354209; ENSP00000346148; ENSG00000132356 [Q13131-2]
ENST00000397128; ENSP00000380317; ENSG00000132356

Database of genes from NCBI RefSeq genomes

More...GeneIDi		5562

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		hsa:5562

Matched Annotation from NCBI and EMBL-EBI (MANE) - Phase one

More...MANE-Selecti		ENST00000397128.7; ENSP00000380317.2; NM_006251.6; NP_006242.5

UCSC genome browser

More...UCSCi		uc003jmb.4, human [Q13131-1]

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC008810 Genomic DNA No translation available.
BC048980 mRNA Translation: AAH48980.1
AB022017 mRNA Translation: BAA36547.1 Different initiation.
AK312947 mRNA Translation: BAG35788.1 Different initiation.
BC037303 mRNA Translation: AAH37303.1 Different initiation.
AF100763 mRNA Translation: AAD43027.1 Different initiation.
U22456 mRNA Translation: AAA64850.1 Different initiation.
Y12856 mRNA Translation: CAA73361.1
CCDSiCCDS3932.2 [Q13131-1]
CCDS3933.2 [Q13131-2]
PIRiG01743
RefSeqiNP_006242.5, NM_006251.5 [Q13131-1]
NP_996790.3, NM_206907.3 [Q13131-2]

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4REDX-ray2.95A/B22-362[»]
4RERX-ray4.05A20-559[»]
4REWX-ray4.58A20-559[»]
5EZVX-ray2.99A/C359-401[»]
6C9FX-ray2.92A22-559[»]
6C9GX-ray2.70A22-559[»]
6C9HX-ray2.65A22-559[»]
6C9JX-ray3.05A22-559[»]
7JHGelectron microscopy3.47A22-559[»]
7JHHelectron microscopy3.92A22-559[»]
7JIJX-ray5.50A22-559[»]
SMRiQ13131
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi111549, 234 interactors
ComplexPortaliCPX-5633, AMPK complex, alpha1-beta1-gamma1 variant
CPX-5786, AMPK complex, alpha1-beta1-gamma2 variant
CPX-5791, AMPK complex, alpha1-beta2-gamma1 variant
CPX-5841, AMPK complex, alpha1-beta2-gamma3 variant
CPX-5842, AMPK complex, alpha1-beta1-gamma3 variant
CPX-5846, AMPK complex, alpha1-beta2-gamma2 variant
CORUMiQ13131
DIPiDIP-39973N
IntActiQ13131, 118 interactors
MINTiQ13131
STRINGi9606.ENSP00000346148

Chemistry databases

BindingDBiQ13131
ChEMBLiCHEMBL4045
DrugBankiDB00945, Acetylsalicylic acid
DB00131, Adenosine phosphate
DB00171, ATP
DB12010, Fostamatinib
DB00914, Phenformin
DB00273, Topiramate
DrugCentraliQ13131

PTM databases

GlyGeniQ13131, 1 site, 1 O-linked glycan (1 site)
iPTMnetiQ13131
PhosphoSitePlusiQ13131

Genetic variation databases

BioMutaiPRKAA1
DMDMi254763436

Proteomic databases

EPDiQ13131
jPOSTiQ13131
MassIVEiQ13131
MaxQBiQ13131
PaxDbiQ13131
PeptideAtlasiQ13131
PRIDEiQ13131
ProteomicsDBi59180 [Q13131-1]
59181 [Q13131-2]

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...Antibodypediai		3564, 1455 antibodies from 45 providers

The DNASU plasmid repository

More...DNASUi		5562

Genome annotation databases

EnsembliENST00000354209; ENSP00000346148; ENSG00000132356 [Q13131-2]
ENST00000397128; ENSP00000380317; ENSG00000132356
GeneIDi5562
KEGGihsa:5562
MANE-SelectiENST00000397128.7; ENSP00000380317.2; NM_006251.6; NP_006242.5
UCSCiuc003jmb.4, human [Q13131-1]

Organism-specific databases

Comparative Toxicogenomics Database

More...CTDi		5562
DisGeNETi5562

GeneCards: human genes, protein and diseases

More...GeneCardsi		PRKAA1
HGNCiHGNC:9376, PRKAA1
HPAiENSG00000132356, Low tissue specificity
MIMi602739, gene
neXtProtiNX_Q13131
OpenTargetsiENSG00000132356
PharmGKBiPA33744
VEuPathDBiHostDB:ENSG00000132356

GenAtlas: human gene database

More...GenAtlasi		Search...

Phylogenomic databases

eggNOGiKOG0583, Eukaryota
GeneTreeiENSGT00940000158865
HOGENOMiCLU_000288_59_3_1
OMAiYRSCQKD
OrthoDBi1127668at2759
PhylomeDBiQ13131
TreeFamiTF314032

Enzyme and pathway databases

BRENDAi2.7.11.1, 2681
2.7.11.31, 2681
PathwayCommonsiQ13131
ReactomeiR-HSA-1632852, Macroautophagy
R-HSA-380972, Energy dependent regulation of mTOR by LKB1-AMPK
R-HSA-5628897, TP53 Regulates Metabolic Genes
R-HSA-6804756, Regulation of TP53 Activity through Phosphorylation
R-HSA-9619483, Activation of AMPK downstream of NMDARs
SignaLinkiQ13131
SIGNORiQ13131

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...BioGRID-ORCSi		5562, 25 hits in 1078 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...ChiTaRSi		PRKAA1, human

The Gene Wiki collection of pages on human genes and proteins

More...GeneWikii		Protein_kinase,_AMP-activated,_alpha_1

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...GenomeRNAii		5562
PharosiQ13131, Tclin

Protein Ontology

More...PROi		PR:Q13131
RNActiQ13131, protein

The Stanford Online Universal Resource for Clones and ESTs

More...SOURCEi		Search...

Gene expression databases

BgeeiENSG00000132356, Expressed in testis and 229 other tissues
ExpressionAtlasiQ13131, baseline and differential
GenevisibleiQ13131, HS

Family and domain databases

CDDicd12199, AMPKA1_C, 1 hit
cd14403, UBA_AID_AAPK1, 1 hit
InterProiView protein in InterPro
IPR032270, AMPK_C
IPR039137, AMPKA1_C
IPR028375, KA1/Ssp2_C
IPR011009, Kinase-like_dom_sf
IPR028797, PRKAA1_UBA
IPR000719, Prot_kinase_dom
IPR017441, Protein_kinase_ATP_BS
IPR008271, Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF16579, AdenylateSensor, 1 hit
PF00069, Pkinase, 1 hit
SMARTiView protein in SMART
SM00220, S_TKc, 1 hit
SUPFAMiSSF103243, SSF103243, 1 hit
SSF56112, SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107, PROTEIN_KINASE_ATP, 1 hit
PS50011, PROTEIN_KINASE_DOM, 1 hit
PS00108, PROTEIN_KINASE_ST, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiAAPK1_HUMAN
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q13131
Secondary accession number(s): A8MTQ6
, B2R7E1, O00286, Q5D0E1, Q86VS1, Q9UNQ4
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 28, 2009
Last modified: February 23, 2022
This is version 227 of the entry and version 4 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  2. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  3. Human entries with genetic variants
    List of human entries with genetic variants
  4. Human variants curated from literature reports
    Index of human variants curated from literature reports
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families
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