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Protein

Chromatin assembly factor 1 subunit B

Gene

CHAF1B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer.1 Publication

GO - Molecular functioni

  • chromatin binding Source: ProtInc
  • histone binding Source: GO_Central
  • unfolded protein binding Source: ProtInc

GO - Biological processi

Keywordsi

Biological processCell cycle, DNA damage, DNA repair, DNA replication, Transcription, Transcription regulation

Enzyme and pathway databases

SignaLinkiQ13112
SIGNORiQ13112

Names & Taxonomyi

Protein namesi
Recommended name:
Chromatin assembly factor 1 subunit B
Short name:
CAF-1 subunit B
Alternative name(s):
Chromatin assembly factor I p60 subunit
Short name:
CAF-I 60 kDa subunit
Short name:
CAF-I p60
M-phase phosphoprotein 7
Gene namesi
Name:CHAF1B
Synonyms:CAF1A, CAF1P60, MPHOSPH7, MPP7
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 21

Organism-specific databases

EuPathDBiHostDB:ENSG00000159259.7
HGNCiHGNC:1911 CHAF1B
MIMi601245 gene
neXtProtiNX_Q13112

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi8208
OpenTargetsiENSG00000159259
PharmGKBiPA26447

Polymorphism and mutation databases

BioMutaiCHAF1B
DMDMi3121829

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000508961 – 559Chromatin assembly factor 1 subunit BAdd BLAST559

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei394PhosphothreonineCombined sources1
Modified residuei409PhosphoserineCombined sources1
Modified residuei419PhosphothreonineCombined sources1
Modified residuei429PhosphoserineCombined sources1
Modified residuei433PhosphothreonineCombined sources1
Modified residuei458PhosphoserineCombined sources1
Modified residuei494N6-acetyllysineCombined sources1
Modified residuei495PhosphothreonineSequence analysis1
Modified residuei509PhosphothreonineSequence analysis1
Modified residuei521PhosphothreonineSequence analysis1
Modified residuei531PhosphothreonineSequence analysis1
Modified residuei538PhosphoserineCombined sources1

Post-translational modificationi

Differentially phosphorylated during cell cycle. During mitosis the p60 subunit of inactive CAF-1 is hyperphosphorylated and displaced into the cytosol. Progressivly dephosphorylated from G1 to S and G2 phase. Phosphorylated p60 is recruited to chromatin undergoing DNA repair after UV irradiation in G1, S or G2 phases.2 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ13112
MaxQBiQ13112
PaxDbiQ13112
PeptideAtlasiQ13112
PRIDEiQ13112
ProteomicsDBi59163

PTM databases

iPTMnetiQ13112
PhosphoSitePlusiQ13112

Expressioni

Developmental stagei

Active complex is found in G1, S and G2 phases.

Gene expression databases

BgeeiENSG00000159259 Expressed in 132 organ(s), highest expression level in secondary oocyte
CleanExiHS_CHAF1B
HS_MPP7
GenevisibleiQ13112 HS

Organism-specific databases

HPAiCAB033604
HPA021679

Interactioni

Subunit structurei

Subunit of the CAF-1 complex that contains RBBP4, CHAF1B and CHAF1A. CHAF1A binds directly to CHAF1B. Only minor amounts of RBBP4 are complexed with CHAF1A and CHAF1B in G1 phase. In G2 and S phase also monomeric CHAF1B is detected.

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi113846, 52 interactors
ComplexPortaliCPX-569 Chromatin assembly factor 1 complex
CORUMiQ13112
DIPiDIP-29243N
IntActiQ13112, 29 interactors
MINTiQ13112
STRINGi9606.ENSP00000315700

Structurei

3D structure databases

ProteinModelPortaliQ13112
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati11 – 54WD 1Add BLAST44
Repeati64 – 103WD 2Add BLAST40
Repeati127 – 166WD 3Add BLAST40
Repeati169 – 208WD 4Add BLAST40
Repeati228 – 279WD 5Add BLAST52
Repeati299 – 340WD 6Add BLAST42
Repeati344 – 385WD 7Add BLAST42

Sequence similaritiesi

Belongs to the WD repeat HIR1 family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG1009 Eukaryota
ENOG410XPU4 LUCA
GeneTreeiENSGT00550000074968
HOGENOMiHOG000183312
HOVERGENiHBG050780
InParanoidiQ13112
KOiK10751
OMAiYFELRTK
OrthoDBiEOG091G0FIG
PhylomeDBiQ13112
TreeFamiTF313062

Family and domain databases

Gene3Di2.130.10.10, 2 hits
InterProiView protein in InterPro
IPR029129 CAF1_p60_C
IPR001632 Gprotein_B
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF15512 CAF-1_p60_C, 1 hit
PF00400 WD40, 3 hits
PRINTSiPR00319 GPROTEINB
SMARTiView protein in SMART
SM00320 WD40, 5 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 1 hit
PS50082 WD_REPEATS_2, 3 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequencei

Sequence statusi: Complete.

Q13112-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKVITCEIAW HNKEPVYSLD FQHGTAGRIH RLASAGVDTN VRIWKVEKGP
60 70 80 90 100
DGKAIVEFLS NLARHTKAVN VVRFSPTGEI LASGGDDAVI LLWKVNDNKE
110 120 130 140 150
PEQIAFQDED EAQLNKENWT VVKTLRGHLE DVYDICWATD GNLMASASVD
160 170 180 190 200
NTAIIWDVSK GQKISIFNEH KSYVQGVTWD PLGQYVATLS CDRVLRVYSI
210 220 230 240 250
QKKRVAFNVS KMLSGIGAEG EARSYRMFHD DSMKSFFRRL SFTPDGSLLL
260 270 280 290 300
TPAGCVESGE NVMNTTYVFS RKNLKRPIAH LPCPGKATLA VRCCPVYFEL
310 320 330 340 350
RPVVETGVEL MSLPYRLVFA VASEDSVLLY DTQQSFPFGY VSNIHYHTLS
360 370 380 390 400
DISWSSDGAF LAISSTDGYC SFVTFEKDEL GIPLKEKPVL NMRTPDTAKK
410 420 430 440 450
TKSQTHRGSS PGPRPVEGTP ASRTQDPSSP GTTPPQARQA PAPTVIRDPP
460 470 480 490 500
SITPAVKSPL PGPSEEKTLQ PSSQNTKAHP SRRVTLNTLQ AWSKTTPRRI
510 520 530 540 550
NLTPLKTDTP PSSVPTSVIS TPSTEEIQSE TPGDAQGSPP ELKRPRLDEN

KGGTESLDP
Length:559
Mass (Da):61,493
Last modified:November 1, 1996 - v1
Checksum:iAD1846CC81B8DC9F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti494K → N in CAA66915 (PubMed:8885239).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_053387506K → Q. Corresponds to variant dbSNP:rs74900401Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20980 mRNA Translation: AAA76737.1
AP000694 Genomic DNA Translation: BAA89426.1
AP001725 Genomic DNA Translation: BAA95549.1
BC021218 mRNA Translation: AAH21218.1
X98262 mRNA Translation: CAA66915.1
CCDSiCCDS13644.1
PIRiB56731
RefSeqiNP_005432.1, NM_005441.2
XP_016883966.1, XM_017028477.1
XP_016883967.1, XM_017028478.1
UniGeneiHs.75238

Genome annotation databases

EnsembliENST00000314103; ENSP00000315700; ENSG00000159259
GeneIDi8208
KEGGihsa:8208
UCSCiuc002yvj.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20980 mRNA Translation: AAA76737.1
AP000694 Genomic DNA Translation: BAA89426.1
AP001725 Genomic DNA Translation: BAA95549.1
BC021218 mRNA Translation: AAH21218.1
X98262 mRNA Translation: CAA66915.1
CCDSiCCDS13644.1
PIRiB56731
RefSeqiNP_005432.1, NM_005441.2
XP_016883966.1, XM_017028477.1
XP_016883967.1, XM_017028478.1
UniGeneiHs.75238

3D structure databases

ProteinModelPortaliQ13112
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113846, 52 interactors
ComplexPortaliCPX-569 Chromatin assembly factor 1 complex
CORUMiQ13112
DIPiDIP-29243N
IntActiQ13112, 29 interactors
MINTiQ13112
STRINGi9606.ENSP00000315700

PTM databases

iPTMnetiQ13112
PhosphoSitePlusiQ13112

Polymorphism and mutation databases

BioMutaiCHAF1B
DMDMi3121829

Proteomic databases

EPDiQ13112
MaxQBiQ13112
PaxDbiQ13112
PeptideAtlasiQ13112
PRIDEiQ13112
ProteomicsDBi59163

Protocols and materials databases

DNASUi8208
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000314103; ENSP00000315700; ENSG00000159259
GeneIDi8208
KEGGihsa:8208
UCSCiuc002yvj.4 human

Organism-specific databases

CTDi8208
DisGeNETi8208
EuPathDBiHostDB:ENSG00000159259.7
GeneCardsiCHAF1B
HGNCiHGNC:1911 CHAF1B
HPAiCAB033604
HPA021679
MIMi601245 gene
neXtProtiNX_Q13112
OpenTargetsiENSG00000159259
PharmGKBiPA26447
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1009 Eukaryota
ENOG410XPU4 LUCA
GeneTreeiENSGT00550000074968
HOGENOMiHOG000183312
HOVERGENiHBG050780
InParanoidiQ13112
KOiK10751
OMAiYFELRTK
OrthoDBiEOG091G0FIG
PhylomeDBiQ13112
TreeFamiTF313062

Enzyme and pathway databases

SignaLinkiQ13112
SIGNORiQ13112

Miscellaneous databases

ChiTaRSiCHAF1B human
GeneWikiiCHAF1B
GenomeRNAii8208
PROiPR:Q13112
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000159259 Expressed in 132 organ(s), highest expression level in secondary oocyte
CleanExiHS_CHAF1B
HS_MPP7
GenevisibleiQ13112 HS

Family and domain databases

Gene3Di2.130.10.10, 2 hits
InterProiView protein in InterPro
IPR029129 CAF1_p60_C
IPR001632 Gprotein_B
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF15512 CAF-1_p60_C, 1 hit
PF00400 WD40, 3 hits
PRINTSiPR00319 GPROTEINB
SMARTiView protein in SMART
SM00320 WD40, 5 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 1 hit
PS50082 WD_REPEATS_2, 3 hits
PS50294 WD_REPEATS_REGION, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCAF1B_HUMAN
AccessioniPrimary (citable) accession number: Q13112
Secondary accession number(s): Q99548
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: November 7, 2018
This is version 195 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 21
    Human chromosome 21: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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