UniProtKB - Q13111 (CAF1A_HUMAN)
Chromatin assembly factor 1 subunit A
CHAF1A
Functioni
Core component of the CAF-1 complex, a complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci.
By similarityCaution
GO - Molecular functioni
- chromatin binding Source: ProtInc
- chromo shadow domain binding Source: BHF-UCL
- identical protein binding Source: IntAct
- unfolded protein binding Source: ProtInc
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- chromatin assembly Source: UniProtKB
- DNA repair Source: UniProtKB-KW
- DNA replication Source: UniProtKB-KW
- DNA replication-dependent chromatin assembly Source: UniProtKB
- nucleosome assembly Source: GO_Central
Keywordsi
Molecular function | Chaperone |
Biological process | Cell cycle, DNA damage, DNA repair, DNA replication |
Enzyme and pathway databases
PathwayCommonsi | Q13111 |
SignaLinki | Q13111 |
SIGNORi | Q13111 |
Names & Taxonomyi
Protein namesi | Recommended name: Chromatin assembly factor 1 subunit ACuratedShort name: CAF-1 subunit A Alternative name(s): Chromatin assembly factor I p150 subunit Short name: CAF-I 150 kDa subunit Short name: CAF-I p150 Short name: hp150 |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:1910, CHAF1A |
MIMi | 601246, gene |
neXtProti | NX_Q13111 |
VEuPathDBi | HostDB:ENSG00000167670 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Note: DNA replication foci.
Nucleus
- nucleus Source: GO_Central
Other locations
- CAF-1 complex Source: UniProtKB
- chromatin Source: UniProtKB
- protein-containing complex Source: UniProtKB
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 240 | V → E: Abolishes interaction with CBX5; when associated with E-242. 1 Publication | 1 | |
Mutagenesisi | 242 | L → E: Abolishes interaction with CBX5; when associated with E-240. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 10036 |
OpenTargetsi | ENSG00000167670 |
PharmGKBi | PA26446 |
Miscellaneous databases
Pharosi | Q13111, Tbio |
Genetic variation databases
BioMutai | CHAF1A |
DMDMi | 229462842 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000089276 | 1 – 956 | Chromatin assembly factor 1 subunit AAdd BLAST | 956 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 65 | PhosphoserineCombined sources | 1 | |
Modified residuei | 123 | PhosphoserineCombined sources | 1 | |
Modified residuei | 138 | PhosphoserineCombined sources | 1 | |
Modified residuei | 141 | PhosphoserineCombined sources | 1 | |
Modified residuei | 143 | PhosphoserineCombined sources | 1 | |
Cross-linki | 182 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateCombined sources | ||
Cross-linki | 182 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources | ||
Modified residuei | 206 | PhosphoserineCombined sources | 1 | |
Modified residuei | 224 | PhosphoserineCombined sources | 1 | |
Modified residuei | 310 | PhosphoserineCombined sources | 1 | |
Modified residuei | 722 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 772 | PhosphoserineCombined sources | 1 | |
Modified residuei | 775 | PhosphoserineCombined sources | 1 | |
Modified residuei | 803 | PhosphoserineCombined sources | 1 | |
Modified residuei | 865 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 868 | PhosphoserineCombined sources | 1 | |
Modified residuei | 873 | PhosphoserineCombined sources | 1 | |
Modified residuei | 951 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q13111 |
jPOSTi | Q13111 |
MassIVEi | Q13111 |
MaxQBi | Q13111 |
PaxDbi | Q13111 |
PeptideAtlasi | Q13111 |
PRIDEi | Q13111 |
ProteomicsDBi | 59160 [Q13111-1] 59161 [Q13111-2] 59162 [Q13111-3] |
PTM databases
GlyGeni | Q13111, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q13111 |
PhosphoSitePlusi | Q13111 |
Expressioni
Developmental stagei
Gene expression databases
Bgeei | ENSG00000167670, Expressed in secondary oocyte and 232 other tissues |
ExpressionAtlasi | Q13111, baseline and differential |
Genevisiblei | Q13111, HS |
Organism-specific databases
HPAi | ENSG00000167670, Tissue enhanced (bone) |
Interactioni
Subunit structurei
Homodimer. Part of the CAF-1 complex that contains RBBP4, CHAF1B and CHAF1A. CHAF1A binds directly to CHAF1B. Only minor amounts of RBBP4 are complexed with CHAF1A and CHAF1B in G1 phase. CHAF1A binds directly to PCNA and to CBX1.
Interacts with MBD1 (PubMed:12697822).
Interacts directly with CBX5 via the PxVxL motif.
Interacts with CBX5.
Interacts with histones H3.1, H3.2 and H3.1t (PubMed:33857403).
6 PublicationsBinary interactionsi
Q13111
GO - Molecular functioni
- chromo shadow domain binding Source: BHF-UCL
- identical protein binding Source: IntAct
- unfolded protein binding Source: ProtInc
Protein-protein interaction databases
BioGRIDi | 115349, 170 interactors |
ComplexPortali | CPX-569, Chromatin assembly factor 1 complex |
CORUMi | Q13111 |
DIPi | DIP-31135N |
ELMi | Q13111 |
IntActi | Q13111, 63 interactors |
MINTi | Q13111 |
STRINGi | 9606.ENSP00000301280 |
Miscellaneous databases
RNActi | Q13111, protein |
Structurei
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 314 | Binds to CBX1 chromo shadow domainAdd BLAST | 314 | |
Regioni | 1 – 49 | Binds to PCNAAdd BLAST | 49 | |
Regioni | 45 – 65 | DisorderedSequence analysisAdd BLAST | 21 | |
Regioni | 122 – 155 | DisorderedSequence analysisAdd BLAST | 34 | |
Regioni | 188 – 222 | DisorderedSequence analysisAdd BLAST | 35 | |
Regioni | 250 – 432 | DisorderedSequence analysisAdd BLAST | 183 | |
Regioni | 599 – 639 | DisorderedSequence analysisAdd BLAST | 41 | |
Regioni | 642 – 678 | Necessary for homodimerization and competence for chromatin assemblyAdd BLAST | 37 | |
Regioni | 660 – 956 | Binds to p60Add BLAST | 297 | |
Regioni | 765 – 790 | DisorderedSequence analysisAdd BLAST | 26 | |
Regioni | 844 – 873 | DisorderedSequence analysisAdd BLAST | 30 | |
Regioni | 933 – 956 | DisorderedSequence analysisAdd BLAST | 24 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 233 – 246 | PxVxL motifAdd BLAST | 14 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 279 – 294 | Polar residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 324 – 432 | Basic and acidic residuesSequence analysisAdd BLAST | 109 | |
Compositional biasi | 599 – 633 | Acidic residuesSequence analysisAdd BLAST | 35 | |
Compositional biasi | 768 – 785 | Polar residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 844 – 870 | Polar residuesSequence analysisAdd BLAST | 27 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG4364, Eukaryota |
GeneTreei | ENSGT00440000034888 |
HOGENOMi | CLU_014846_0_0_1 |
InParanoidi | Q13111 |
OMAi | RRRGMDC |
OrthoDBi | 1362011at2759 |
PhylomeDBi | Q13111 |
TreeFami | TF350377 |
Family and domain databases
InterProi | View protein in InterPro IPR029105, CAF1-p150_C2 IPR029091, CAF1_p150_N IPR022043, CAF1A |
Pfami | View protein in Pfam PF15539, CAF1-p150_C2, 1 hit PF15557, CAF1-p150_N, 1 hit PF12253, CAF1A, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MLEELECGAP GARGAATAMD CKDRPAFPVK KLIQARLPFK RLNLVPKGKA
60 70 80 90 100
DDMSDDQGTS VQSKSPDLEA SLDTLENNCH VGSDIDFRPK LVNGKGPLDN
110 120 130 140 150
FLRNRIETSI GQSTVIIDLT EDSNEQPDSL VDHNKLNSEA SPSREAINGQ
160 170 180 190 200
REDTGDQQGL LKAIQNDKLA FPGETLSDIP CKTEEEGVGC GGAGRRGDSQ
210 220 230 240 250
ECSPRSCPEL TSGPRMCPRK EQDSWSEAGG ILFKGKVPMV VLQDILAVRP
260 270 280 290 300
PQIKSLPATP QGKNMTPESE VLESFPEEDS VLSHSSLSSP SSTSSPEGPP
310 320 330 340 350
APPKQHSSTS PFPTSTPLRR ITKKFVKGST EKNKLRLQRD QERLGKQLKL
360 370 380 390 400
RAEREEKEKL KEEAKRAKEE AKKKKEEEKE LKEKERREKR EKDEKEKAEK
410 420 430 440 450
QRLKEERRKE RQEALEAKLE EKRKKEEEKR LREEEKRIKA EKAEITRFFQ
460 470 480 490 500
KPKTPQAPKT LAGSCGKFAP FEIKEHMVLA PRRRTAFHPD LCSQLDQLLQ
510 520 530 540 550
QQSGEFSFLK DLKGRQPLRS GPTHVSTRNA DIFNSDVVIV ERGKGDGVPE
560 570 580 590 600
RRKFGRMKLL QFCENHRPAY WGTWNKKTAL IRARDPWAQD TKLLDYEVDS
610 620 630 640 650
DEEWEEEEPG ESLSHSEGDD DDDMGEDEDE DDGFFVPHGY LSEDEGVTEE
660 670 680 690 700
CADPENHKVR QKLKAKEWDE FLAKGKRFRV LQPVKIGCVW AADRDCAGDD
710 720 730 740 750
LKVLQQFAAC FLETLPAQEE QTPKASKRER RDEQILAQLL PLLHGNVNGS
760 770 780 790 800
KVIIREFQEH CRRGLLSNHT GSPRSPSTTY LHTPTPSEDA AIPSKSRLKR
810 820 830 840 850
LISENSVYEK RPDFRMCWYV HPQVLQSFQQ EHLPVPCQWS YVTSVPSAPK
860 870 880 890 900
EDSGSVPSTG PSQGTPISLK RKSAGSMCIT QFMKKRRHDG QIGAEDMDGF
910 920 930 940 950
QADTEEEEEE EGDCMIVDVP DAAEVQAPCG AASGAGGGVG VDTGKATLTA
SPLGAS
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketK7EPA1 | K7EPA1_HUMAN | Chromatin assembly factor 1 subunit... | CHAF1A | 291 | Annotation score: | ||
K7EJF1 | K7EJF1_HUMAN | Chromatin assembly factor 1 subunit... | CHAF1A | 151 | Annotation score: | ||
K7EME9 | K7EME9_HUMAN | Chromatin assembly factor 1 subunit... | CHAF1A | 47 | Annotation score: | ||
K7ENC7 | K7ENC7_HUMAN | Chromatin assembly factor 1 subunit... | CHAF1A | 17 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 775 | S → T in AAA76736 (PubMed:7600578).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_055329 | 167 | D → V. Corresponds to variant dbSNP:rs35651457Ensembl. | 1 | |
Natural variantiVAR_055330 | 239 | M → V. Corresponds to variant dbSNP:rs2230635Ensembl. | 1 | |
Natural variantiVAR_055331 | 850 | K → R. Corresponds to variant dbSNP:rs8100525Ensembl. | 1 | |
Natural variantiVAR_055332 | 923 | A → V2 PublicationsCorresponds to variant dbSNP:rs9352Ensembl. | 1 | |
Natural variantiVAR_055333 | 950 | A → S. Corresponds to variant dbSNP:rs243383Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_004149 | 710 – 749 | CFLET…GNVNG → HWVHPESRGDVCRTLRVSSP QSRYLNRLNSCVKSTLSCFT in isoform 2. CuratedAdd BLAST | 40 | |
Alternative sequenceiVSP_004150 | 750 – 956 | Missing in isoform 2. CuratedAdd BLAST | 207 | |
Alternative sequenceiVSP_004151 | 772 – 944 | Missing in isoform 3. CuratedAdd BLAST | 173 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF190465 Genomic DNA Translation: AAF04291.1 Different initiation. AC011498 Genomic DNA No translation available. KF459577 Genomic DNA No translation available. BC052620 mRNA Translation: AAH52620.1 Sequence problems. BC067093 mRNA Translation: AAH67093.1 U20979 mRNA Translation: AAA76736.1 Different initiation. |
CCDSi | CCDS32875.1 [Q13111-1] |
PIRi | A56731 |
RefSeqi | NP_005474.2, NM_005483.2 [Q13111-1] |
Genome annotation databases
Ensembli | ENST00000301280; ENSP00000301280; ENSG00000167670 |
GeneIDi | 10036 |
KEGGi | hsa:10036 |
MANE-Selecti | ENST00000301280.10; ENSP00000301280.4; NM_005483.3; NP_005474.2 |
UCSCi | uc002mal.4, human [Q13111-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF190465 Genomic DNA Translation: AAF04291.1 Different initiation. AC011498 Genomic DNA No translation available. KF459577 Genomic DNA No translation available. BC052620 mRNA Translation: AAH52620.1 Sequence problems. BC067093 mRNA Translation: AAH67093.1 U20979 mRNA Translation: AAA76736.1 Different initiation. |
CCDSi | CCDS32875.1 [Q13111-1] |
PIRi | A56731 |
RefSeqi | NP_005474.2, NM_005483.2 [Q13111-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 115349, 170 interactors |
ComplexPortali | CPX-569, Chromatin assembly factor 1 complex |
CORUMi | Q13111 |
DIPi | DIP-31135N |
ELMi | Q13111 |
IntActi | Q13111, 63 interactors |
MINTi | Q13111 |
STRINGi | 9606.ENSP00000301280 |
PTM databases
GlyGeni | Q13111, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q13111 |
PhosphoSitePlusi | Q13111 |
Genetic variation databases
BioMutai | CHAF1A |
DMDMi | 229462842 |
Proteomic databases
EPDi | Q13111 |
jPOSTi | Q13111 |
MassIVEi | Q13111 |
MaxQBi | Q13111 |
PaxDbi | Q13111 |
PeptideAtlasi | Q13111 |
PRIDEi | Q13111 |
ProteomicsDBi | 59160 [Q13111-1] 59161 [Q13111-2] 59162 [Q13111-3] |
Protocols and materials databases
Antibodypediai | 4198, 234 antibodies from 33 providers |
DNASUi | 10036 |
Genome annotation databases
Ensembli | ENST00000301280; ENSP00000301280; ENSG00000167670 |
GeneIDi | 10036 |
KEGGi | hsa:10036 |
MANE-Selecti | ENST00000301280.10; ENSP00000301280.4; NM_005483.3; NP_005474.2 |
UCSCi | uc002mal.4, human [Q13111-1] |
Organism-specific databases
CTDi | 10036 |
DisGeNETi | 10036 |
GeneCardsi | CHAF1A |
HGNCi | HGNC:1910, CHAF1A |
HPAi | ENSG00000167670, Tissue enhanced (bone) |
MIMi | 601246, gene |
neXtProti | NX_Q13111 |
OpenTargetsi | ENSG00000167670 |
PharmGKBi | PA26446 |
VEuPathDBi | HostDB:ENSG00000167670 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4364, Eukaryota |
GeneTreei | ENSGT00440000034888 |
HOGENOMi | CLU_014846_0_0_1 |
InParanoidi | Q13111 |
OMAi | RRRGMDC |
OrthoDBi | 1362011at2759 |
PhylomeDBi | Q13111 |
TreeFami | TF350377 |
Enzyme and pathway databases
PathwayCommonsi | Q13111 |
SignaLinki | Q13111 |
SIGNORi | Q13111 |
Miscellaneous databases
BioGRID-ORCSi | 10036, 733 hits in 1060 CRISPR screens |
ChiTaRSi | CHAF1A, human |
GeneWikii | CHAF1A |
GenomeRNAii | 10036 |
Pharosi | Q13111, Tbio |
PROi | PR:Q13111 |
RNActi | Q13111, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000167670, Expressed in secondary oocyte and 232 other tissues |
ExpressionAtlasi | Q13111, baseline and differential |
Genevisiblei | Q13111, HS |
Family and domain databases
InterProi | View protein in InterPro IPR029105, CAF1-p150_C2 IPR029091, CAF1_p150_N IPR022043, CAF1A |
Pfami | View protein in Pfam PF15539, CAF1-p150_C2, 1 hit PF15557, CAF1-p150_N, 1 hit PF12253, CAF1A, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CAF1A_HUMAN | |
Accessioni | Q13111Primary (citable) accession number: Q13111 Secondary accession number(s): Q6NXG5, Q7Z7K3, Q9UJY8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 18, 2001 |
Last sequence update: | March 28, 2018 | |
Last modified: | February 23, 2022 | |
This is version 196 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families