Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

UniProtKB - Q13111 (CAF1A_HUMAN)

(max 400 entries)x

Your basket is currently empty. i

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Chromatin assembly factor 1 subunit A

Gene

CHAF1A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci (By similarity).By similarity1 Publication

GO - Molecular functioni

  • chromatin binding Source: ProtInc
  • chromo shadow domain binding Source: BHF-UCL
  • identical protein binding Source: IntAct
  • unfolded protein binding Source: ProtInc
View the complete GO annotation on QuickGO ...

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • chromatin assembly Source: UniProtKB
  • DNA repair Source: UniProtKB-KW
  • DNA replication Source: UniProtKB-KW
  • DNA replication-dependent nucleosome assembly Source: UniProtKB
View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionChaperone
Biological processCell cycle, DNA damage, DNA repair, DNA replication

Enzyme and pathway databases

SIGNORiQ13111

Names & Taxonomyi

Protein namesi
Recommended name:
Chromatin assembly factor 1 subunit A
Short name:
CAF-1 subunit A
Alternative name(s):
Chromatin assembly factor I p150 subunit
Short name:
CAF-I 150 kDa subunit
Short name:
CAF-I p150
Short name:
hp150
Gene namesi
Name:CHAF1A
Synonyms:CAF, CAF1P150
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000167670.15
HGNCiHGNC:1910 CHAF1A
MIMi601246 gene
neXtProtiNX_Q13111

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi240V → E: Abolishes interaction with CBX5; when associated with E-242. 1 Publication1
Mutagenesisi242L → E: Abolishes interaction with CBX5; when associated with E-240. 1 Publication1

Organism-specific databases

DisGeNETi10036
OpenTargetsiENSG00000167670
PharmGKBiPA26446

Polymorphism and mutation databases

BioMutaiCHAF1A
DMDMi229462842

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000892761 – 956Chromatin assembly factor 1 subunit AAdd BLAST956

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei65PhosphoserineCombined sources1
Modified residuei123PhosphoserineCombined sources1
Modified residuei138PhosphoserineCombined sources1
Modified residuei141PhosphoserineCombined sources1
Modified residuei143PhosphoserineCombined sources1
Cross-linki182Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateCombined sources
Cross-linki182Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Modified residuei206PhosphoserineCombined sources1
Modified residuei224PhosphoserineCombined sources1
Modified residuei310PhosphoserineCombined sources1
Modified residuei722PhosphothreonineCombined sources1
Modified residuei772PhosphoserineCombined sources1
Modified residuei775PhosphoserineCombined sources1
Modified residuei803PhosphoserineCombined sources1
Modified residuei865PhosphothreonineCombined sources1
Modified residuei868PhosphoserineCombined sources1
Modified residuei873PhosphoserineCombined sources1
Modified residuei951PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ13111
MaxQBiQ13111
PaxDbiQ13111
PeptideAtlasiQ13111
PRIDEiQ13111
ProteomicsDBi59160
59161 [Q13111-2]
59162 [Q13111-3]

PTM databases

iPTMnetiQ13111
PhosphoSitePlusiQ13111

Miscellaneous databases

PMAP-CutDBiQ13111

Expressioni

Developmental stagei

Active complex is found in G1, S and G2 phases.

Gene expression databases

BgeeiENSG00000167670
CleanExiHS_CHAF1A
ExpressionAtlasiQ13111 baseline and differential
GenevisibleiQ13111 HS

Organism-specific databases

HPAiCAB015186

Interactioni

Subunit structurei

Homodimer. Part of the CAF-1 complex that contains RBBP4, CHAF1B and CHAF1A. CHAF1A binds directly to CHAF1B. Only minor amounts of RBBP4 are complexed with CHAF1A and CHAF1B in G1 phase. CHAF1A binds directly to PCNA and to CBX1. Binds MBD1. Interacts directly with CBX5 via the PxVxL motif. During DNA replication, it forms a S phase-specific complex that facilitates DNA methylation and histone H3 'Lys-9' methylation during replication-coupled chromatin assembly and is at least composed of the CHAF1A, MBD1 and SETDB1. Interacts with CBX5.6 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • chromo shadow domain binding Source: BHF-UCL
  • identical protein binding Source: IntAct
  • unfolded protein binding Source: ProtInc
View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridi115349, 81 interactors
ComplexPortaliCPX-569 Chromatin assembly factor 1 complex
CORUMiQ13111
DIPiDIP-31135N
ELMiQ13111
IntActiQ13111, 53 interactors
MINTiQ13111
STRINGi9606.ENSP00000301280

Structurei

3D structure databases

ProteinModelPortaliQ13111
SMRiQ13111
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 314Binds to CBX1 chromo shadow domainAdd BLAST314
Regioni1 – 49Binds to PCNAAdd BLAST49
Regioni642 – 678Necessary for homodimerization and competence for chromatin assemblyAdd BLAST37
Regioni660 – 956Binds to p60Add BLAST297

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi233 – 246PxVxL motifAdd BLAST14

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi323 – 453Arg/Glu/Lys-richAdd BLAST131
Compositional biasi602 – 608Poly-Glu7
Compositional biasi619 – 623Poly-Asp5
Compositional biasi905 – 911Poly-Glu7

Domaini

Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.

Sequence similaritiesi

Belongs to the CHAF1A family.Curated

Phylogenomic databases

eggNOGiKOG4364 Eukaryota
ENOG4111K9K LUCA
GeneTreeiENSGT00440000034888
HOGENOMiHOG000111290
HOVERGENiHBG050779
InParanoidiQ13111
KOiK10750
OrthoDBiEOG091G0VQK
PhylomeDBiQ13111
TreeFamiTF350377

Family and domain databases

InterProiView protein in InterPro
IPR029105 CAF1-p150_C2
IPR029091 CAF1_asu_N
IPR022043 CAF1A
PfamiView protein in Pfam
PF15539 CAF1-p150_C2, 1 hit
PF15557 CAF1-p150_N, 1 hit
PF12253 CAF1A, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Experimental confirmation may be lacking for some isoforms.
Isoform 1 (identifier: Q13111-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLEELECGAP GARGAATAMD CKDRPAFPVK KLIQARLPFK RLNLVPKGKA 
        60         70         80         90        100
DDMSDDQGTS VQSKSPDLEA SLDTLENNCH VGSDIDFRPK LVNGKGPLDN 
       110        120        130        140        150
FLRNRIETSI GQSTVIIDLT EDSNEQPDSL VDHNKLNSEA SPSREAINGQ 
       160        170        180        190        200
REDTGDQQGL LKAIQNDKLA FPGETLSDIP CKTEEEGVGC GGAGRRGDSQ 
       210        220        230        240        250
ECSPRSCPEL TSGPRMCPRK EQDSWSEAGG ILFKGKVPMV VLQDILAVRP 
       260        270        280        290        300
PQIKSLPATP QGKNMTPESE VLESFPEEDS VLSHSSLSSP SSTSSPEGPP 
       310        320        330        340        350
APPKQHSSTS PFPTSTPLRR ITKKFVKGST EKNKLRLQRD QERLGKQLKL 
       360        370        380        390        400
RAEREEKEKL KEEAKRAKEE AKKKKEEEKE LKEKERREKR EKDEKEKAEK 
       410        420        430        440        450
QRLKEERRKE RQEALEAKLE EKRKKEEEKR LREEEKRIKA EKAEITRFFQ 
       460        470        480        490        500
KPKTPQAPKT LAGSCGKFAP FEIKEHMVLA PRRRTAFHPD LCSQLDQLLQ 
       510        520        530        540        550
QQSGEFSFLK DLKGRQPLRS GPTHVSTRNA DIFNSDVVIV ERGKGDGVPE 
       560        570        580        590        600
RRKFGRMKLL QFCENHRPAY WGTWNKKTAL IRARDPWAQD TKLLDYEVDS 
       610        620        630        640        650
DEEWEEEEPG ESLSHSEGDD DDDMGEDEDE DDGFFVPHGY LSEDEGVTEE 
       660        670        680        690        700
CADPENHKVR QKLKAKEWDE FLAKGKRFRV LQPVKIGCVW AADRDCAGDD 
       710        720        730        740        750
LKVLQQFAAC FLETLPAQEE QTPKASKRER RDEQILAQLL PLLHGNVNGS 
       760        770        780        790        800
KVIIREFQEH CRRGLLSNHT GSPRSPSTTY LHTPTPSEDA AIPSKSRLKR 
       810        820        830        840        850
LISENSVYEK RPDFRMCWYV HPQVLQSFQQ EHLPVPCQWS YVTSVPSAPK 
       860        870        880        890        900
EDSGSVPSTG PSQGTPISLK RKSAGSMCIT QFMKKRRHDG QIGAEDMDGF 
       910        920        930        940        950
QADTEEEEEE EGDCMIVDVP DAAEVQAPCG AASGAGGGVG VDTGKATLTA 

SPLGAS
Length:956
Mass (Da):106,910
Last modified:March 28, 2018 - v3
Checksum:i90617F8FE8DB7FD0
GO
Isoform 2 (identifier: Q13111-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     710-749: CFLETLPAQE...LPLLHGNVNG → HWVHPESRGD...CVKSTLSCFT
     750-956: Missing.

Show »
Length:749
Mass (Da):84,685
Checksum:iA7176E2D8D18CA0F
GO
Isoform 3 (identifier: Q13111-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     772-944: Missing.

Show »
Length:783
Mass (Da):88,269
Checksum:i44E39E1CCE193030
GO

Sequence cautioni

The sequence AAA76736 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAF04291 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAH52620 differs from that shown. Contaminating sequence. Potential poly-A sequence starting in position 426.Curated
The sequence AAH52620 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti775S → T in AAA76736 (PubMed:7600578).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_055329167D → V. Corresponds to variant dbSNP:rs35651457Ensembl.1
Natural variantiVAR_055330239M → V. Corresponds to variant dbSNP:rs2230635Ensembl.1
Natural variantiVAR_055331850K → R. Corresponds to variant dbSNP:rs8100525Ensembl.1
Natural variantiVAR_055332923A → V2 PublicationsCorresponds to variant dbSNP:rs9352Ensembl.1
Natural variantiVAR_055333950A → S. Corresponds to variant dbSNP:rs243383Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_004149710 – 749CFLET…GNVNG → HWVHPESRGDVCRTLRVSSP QSRYLNRLNSCVKSTLSCFT in isoform 2. CuratedAdd BLAST40
Alternative sequenceiVSP_004150750 – 956Missing in isoform 2. CuratedAdd BLAST207
Alternative sequenceiVSP_004151772 – 944Missing in isoform 3. CuratedAdd BLAST173

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF190465 Genomic DNA Translation: AAF04291.1 Different initiation.
AC011498 Genomic DNA No translation available.
KF459577 Genomic DNA No translation available.
BC052620 mRNA Translation: AAH52620.1 Sequence problems.
BC067093 mRNA Translation: AAH67093.1
U20979 mRNA Translation: AAA76736.1 Different initiation.
CCDSiCCDS32875.1 [Q13111-1]
PIRiA56731
RefSeqiNP_005474.2, NM_005483.2 [Q13111-1]
UniGeneiHs.79018

Genome annotation databases

EnsembliENST00000301280; ENSP00000301280; ENSG00000167670 [Q13111-1]
GeneIDi10036
KEGGihsa:10036
UCSCiuc002mal.4 human [Q13111-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiCAF1A_HUMAN
AccessioniPrimary (citable) accession number: Q13111
Secondary accession number(s): Q6NXG5, Q7Z7K3, Q9UJY8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: March 28, 2018
Last modified: July 18, 2018
This is version 174 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health